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学者姓名：刁洪秀
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Abstract ：
Vaccination is one of the most effective methods for controlling animal infectious diseases, and the use of adjuvants plays crucial role in enhancing the immune efficacy of vaccines, particularly in inactivated and subunit vaccines. With the continuous advancement of research in animal immunology and immune mechanisms, our understanding of the functions of cells and cytokines in immune responses has become increasingly comprehensive, laying a solid foundation for the development of novel vaccines and adjuvants. Cytokines are a class of proteins secreted by the animal body that regulate innate and adaptive immune responses through interaction with specific receptors. To date, numerous studies have investigated the potential of using cytokines as adjuvants to enhance the efficacy of veterinary vaccines. This review focuses on cytokines as veterinary vaccine adjuvants, with special attention to the current research progress and mechanisms of cytokines such as interleukins, interferons, chemokines, and colony-stimulating factors. Additionally, examples of the application of cytokine-based adjuvants in combination with veterinary vaccines will be discussed to provide further insights and references for the development of cytokine-based veterinary adjuvants.
Keyword ：
cytokines cytokines immune enhancement immune enhancement vaccination vaccination vaccine adjuvant vaccine adjuvant veterinary vaccine veterinary vaccine
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| GB/T 7714 | Yan, Shihong , Chen, Huimin , Liu, Qiao et al. Harnessing cytokines: innovative adjuvants for improved veterinary vaccine efficacy [J]. | FRONTIERS IN IMMUNOLOGY , 2025 , 16 . |
| MLA | Yan, Shihong et al. "Harnessing cytokines: innovative adjuvants for improved veterinary vaccine efficacy" . | FRONTIERS IN IMMUNOLOGY 16 (2025) . |
| APA | Yan, Shihong , Chen, Huimin , Liu, Qiao , Zhang, Xinyu , Wei, Qiulu , Hu, Chenqi et al. Harnessing cytokines: innovative adjuvants for improved veterinary vaccine efficacy . | FRONTIERS IN IMMUNOLOGY , 2025 , 16 . |
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旨在探究阿霉素对犬乳腺肿瘤细胞中lncRNAs分子的影响，寻找犬乳腺肿瘤潜在生物标志物和治疗靶点，本试验采用RNA-Seq技术对经阿霉素处理的犬乳腺肿瘤细胞进行转录组测序分析，筛选差异表达lncRNAs,应用GO富集和KEGG富集分析进行基因功能和途径注释并验证其表达情况。结果显示，经阿霉素处理后的犬乳腺肿瘤细胞中共有277个差异表达的lncRNAs,其中，147个表达上调，130个表达下调，主要涉及细胞新陈代谢、转录程序失调、ECM受体相互作用以及细胞坏死性凋亡等生物学过程。实时荧光定量PCR技术随机检测3个差异表达的lncRNAs,其表达趋势与RNA-Seq一致，表明RNA-Seq测序结果的准确性。本研究基于转录组学技术，综合分析阿霉素抗犬乳腺肿瘤细胞相关lncRNAs表达变化的整体特征，揭示其可能的调控新机制，为犬乳腺肿瘤的靶向治疗和潜在生物标志物的开发利用提供参考。
Keyword ：
lncRNA lncRNA RNA-Seq RNA-Seq 乳腺肿瘤细胞 乳腺肿瘤细胞 犬 犬 阿霉素 阿霉素
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| GB/T 7714 | 张琰 , 吴梅金 , 周家豪 et al. 阿霉素处理后对犬乳腺肿瘤细胞系CHMp lncRNAs差异表达的影响 [J]. | 畜牧兽医学报 , 2024 , 55 (06) : 2716-2726 . |
| MLA | 张琰 et al. "阿霉素处理后对犬乳腺肿瘤细胞系CHMp lncRNAs差异表达的影响" . | 畜牧兽医学报 55 . 06 (2024) : 2716-2726 . |
| APA | 张琰 , 吴梅金 , 周家豪 , 刁洪秀 . 阿霉素处理后对犬乳腺肿瘤细胞系CHMp lncRNAs差异表达的影响 . | 畜牧兽医学报 , 2024 , 55 (06) , 2716-2726 . |
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Vaccine adjuvants, serving as non-specific immune enhancers, play a pivotal role in the immunoprevention and control of animal diseases. This study utilized prokaryotic expression systems to express and purify chickenderived cIFITM1, cIFITM3, and cViperin, which were then formulated as adjuvants with H9N2 avian influenza virus antigens to create inactivated vaccines. These vaccines were administered to SPF chickens to investigate their immunopotentiating functions. Additionally, the proteins were assessed for their ability to act as standalone immune enhancers. The results demonstrated that cIFITM1, cIFITM3, and cViperin significantly elevated serum hemagglutination inhibition (HI) antibody titers. Notably, when used individually, these proteins markedly enhanced the antiviral capabilities of challenged chickens, leading to alleviated clinical symptoms, reduced tracheal virus replication, diminished virus shedding, and lessened histopathological damage, with cIFITM1 exhibiting the most pronounced effect. Furthermore, the protective efficacy of two H9N2 recombinant virus inactivated vaccines supplemented with cIFITM1 adjuvant was validated, achieving a 100% vaccine protection efficiency. In conclusion, cIFITM1, cIFITM3, and cViperin as adjuvants for influenza vaccines effectively inhibit virus replication and shedding, highlighting their significant potential in influenza prevention and control.
Keyword ：
Antiviral Protein Antiviral Protein Avian vaccine adjuvant Avian vaccine adjuvant Protection efficiency Protection efficiency
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| GB/T 7714 | Yan, Shihong , Chen, Yikai , Lin, Jin et al. Recombinant avian-derived antiviral proteins cIFITM1, cIFITM3, and cViperin as effective adjuvants in inactivated H9N2 subtype avian influenza vaccines [J]. | VETERINARY MICROBIOLOGY , 2024 , 298 . |
| MLA | Yan, Shihong et al. "Recombinant avian-derived antiviral proteins cIFITM1, cIFITM3, and cViperin as effective adjuvants in inactivated H9N2 subtype avian influenza vaccines" . | VETERINARY MICROBIOLOGY 298 (2024) . |
| APA | Yan, Shihong , Chen, Yikai , Lin, Jin , Chen, Huimin , Hu, Chenqi , Liu, Hongyang et al. Recombinant avian-derived antiviral proteins cIFITM1, cIFITM3, and cViperin as effective adjuvants in inactivated H9N2 subtype avian influenza vaccines . | VETERINARY MICROBIOLOGY , 2024 , 298 . |
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Animal experiments have played a significant role in advancing scientific knowledge and enhancing people's quality of life. In order to better understand the opinions and knowledge of veterinary students in the domain of laboratory animal welfare and to explore and advance the teaching methods used in animal ethics education, a questionnaire was designed and used to conduct a survey among undergraduate and postgraduate students majoring in veterinary medicine. The survey encompassed various topics, such as students' level of knowledge about animal welfare, their perspectives on laboratory animals, their proficiency with animal experiments, and their opinions on teaching methods and content. The respondents were a total of 150 undergraduate students and 148 graduate students. The survey results indicated that most students expressed a strong sense of responsibility for the safeguarding of the welfare of experimental animals. However, there were a few students who lacked compassion for animals. Additionally, there was a general lack of basic theoretical knowledge of animal ethics and an inadequate grasp of experimental techniques among current students. Furthermore, most of the participants expressed a strong sense of responsibility to advocate for animal welfare. Although a substantial number of students were unaware of the existence of agencies for the supervision of work involving laboratory animals, they supported teaching and supervision in the domain of animal welfare and were open to various teaching methods and topics of content. In conclusion, targeted training and education regarding laboratory animal welfare and ethics should be conducted in the future to address the specific needs of students. This study provides a foundation for future animal welfare education and will help to improve the professional skills and humanistic qualities of veterinary students.
Keyword ：
animal welfare animal welfare Chinese veterinary students Chinese veterinary students laboratory animal laboratory animal survey research survey research university education university education
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| GB/T 7714 | Yan, Shihong , Li, Hongyang , Lin, Jin et al. An investigation of the perceptions of laboratory animal welfare issues among undergraduate and graduate veterinary students in southeastern China [J]. | FRONTIERS IN VETERINARY SCIENCE , 2024 , 10 . |
| MLA | Yan, Shihong et al. "An investigation of the perceptions of laboratory animal welfare issues among undergraduate and graduate veterinary students in southeastern China" . | FRONTIERS IN VETERINARY SCIENCE 10 (2024) . |
| APA | Yan, Shihong , Li, Hongyang , Lin, Jin , Chen, Huimin , Liu, Shasha , Diao, Hongxiu . An investigation of the perceptions of laboratory animal welfare issues among undergraduate and graduate veterinary students in southeastern China . | FRONTIERS IN VETERINARY SCIENCE , 2024 , 10 . |
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Simple Summary Mammary tumors are the most prevalent type of tumor in the canine population. C6 ceramide has been identified as an essential factor in various functions in cancer; however, the role of C6 ceramide in canine mammary cancer remains unknown. Therefore, we investigated the role of C6 ceramide in the progress of canine mammary cancer and explored its potential mechanism. The findings show that C6 ceramide inhibited cell growth, migration, and invasion in vitro. C6 ceramide decreased tumor growth and metastasis in the lungs without side effects in xenograft models. Further exploring found that EGR3 is a target agent of C6 ceramide, promoting canine mammary cancer cell proliferation and migration by activating the pJAK1/pSTAT3 signaling pathway. This study revealed the anti-tumor activity of C6 ceramide in canine mammary cancer both in vivo and in vitro and revealed that EGR3 is a potential biomarker in canine mammary cancer prognosis and treatment.Abstract Cancer is the leading cause of death in both humans and companion animals. Canine mammary tumor is an important disease with a high incidence and metastasis rate, and its poor prognosis remains a serious clinical challenge. C6 ceramide is a short-chain sphingolipid metabolite with powerful potential as a tumor suppressor. However, the specific impact of C6 ceramide on canine mammary cancer remains unclear. However, the effects of C6 ceramide in canine mammary cancer are still unclear. Therefore, we investigated the role of C6 ceramide in the progress of canine mammary cancer and explored its potential mechanism. C6 ceramide inhibited cell growth by regulating the cell cycle without involving apoptosis. Additionally, C6 ceramide inhibited the migration and invasion of CHMp cells. In vivo, C6 ceramide decreased tumor growth and metastasis in the lungs without side effects. Further investigation found that the knockdown of EGR3 expression led to a noticeable increase in proliferation and migration by upregulating the expressions of pJAK1 and pSTAT3, thus activating the JAK1/STAT3 signaling pathway. In conclusion, C6 ceramide inhibits canine mammary cancer growth and metastasis by targeting EGR3 through the regulation of the JAK1/STAT3 signaling pathway. This study implicates the mechanisms underlying the anti-tumor activity of C6 ceramide and demonstrates the potential of EGR3 as a novel target for treating canine mammary cancer.
Keyword ：
anti-tumor activity anti-tumor activity C6 ceramide C6 ceramide dog dog EGR3 EGR3 JAK1/STAT3 signaling JAK1/STAT3 signaling mammary tumor mammary tumor
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| GB/T 7714 | Liu, Jiayue , Zhao, Fangying , Zhang, Yan et al. C6 Ceramide Inhibits Canine Mammary Cancer Growth and Metastasis by Targeting EGR3 through JAK1/STAT3 Signaling [J]. | ANIMALS , 2024 , 14 (3) . |
| MLA | Liu, Jiayue et al. "C6 Ceramide Inhibits Canine Mammary Cancer Growth and Metastasis by Targeting EGR3 through JAK1/STAT3 Signaling" . | ANIMALS 14 . 3 (2024) . |
| APA | Liu, Jiayue , Zhao, Fangying , Zhang, Yan , Lin, Zhaoyan , Chen, Ji-Long , Diao, Hongxiu . C6 Ceramide Inhibits Canine Mammary Cancer Growth and Metastasis by Targeting EGR3 through JAK1/STAT3 Signaling . | ANIMALS , 2024 , 14 (3) . |
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Canine mammary carcinomas (CMCs) represent the most prevalent form of cancer in female dogs, characterized by a high incidence and mortality rate. C6 ceramide is recognized for its multifaceted anti-cancer properties, yet its specific influence on CMCs remains to be elucidated. Long noncoding RNAs (lncRNAs), now recognized as functional "dark matter" in precision oncology, are particularly intriguing, with 44% of canine lncRNAs exhibiting tissue-specific expression. In this study, we performed a thorough analysis of lncRNA expression profiles to uncover the mechanisms behind C6 ceramide's anti-cancer activity in CHMp cells. Our findings reveal that C6 ceramide notably inhibits the proliferation of CHMp cells. RNA sequencing identified 4522 lncRNAs with expression changes following C6 ceramide treatment, of which 2936 were upregulated and 1586 were downregulated. Further investigation into Lnc_025370 showed that it is predominantly nuclear-localized and is significantly downregulated by C6 ceramide treatment. Functional studies discovered that overexpression of Lnc_025370 enhances the growth and metastatic capabilities of CHMp cells, which is associated with an increase in NRG1, and concurrently diminishes the anti-cancer effectiveness of C6 ceramide in vitro. Mouse xenograft models also showed that Lnc_025370 overexpression promotes tumor growth and Ki67 expression. Together, our results suggest that Lnc_025370 acts as a pivotal target mediator of C6 ceramide's anti-cancer effects, facilitating the malignant progression of CHMp cells.
Keyword ：
C6 ceramide C6 ceramide canine mammary carcinomas canine mammary carcinomas expression profiles expression profiles Lnc_025370 Lnc_025370 NRG1 NRG1
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| GB/T 7714 | Diao, Hongxiu , Zhao, Fangying , Wu, Meijin et al. LncRNA Expression Profiles in C6 Ceramide Treatment Reveal lnc_025370 as a Promoter in Canine Mammary Carcinoma CHMp Cells Progression [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2024 , 46 (12) : 14190-14203 . |
| MLA | Diao, Hongxiu et al. "LncRNA Expression Profiles in C6 Ceramide Treatment Reveal lnc_025370 as a Promoter in Canine Mammary Carcinoma CHMp Cells Progression" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 46 . 12 (2024) : 14190-14203 . |
| APA | Diao, Hongxiu , Zhao, Fangying , Wu, Meijin , Zhang, Yan , Tao, Qianting , Chen, Shichao et al. LncRNA Expression Profiles in C6 Ceramide Treatment Reveal lnc_025370 as a Promoter in Canine Mammary Carcinoma CHMp Cells Progression . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2024 , 46 (12) , 14190-14203 . |
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Simple Summary Cancer is the leading cause of death in dogs. Currently, researchers find long non-coding RNA (lncRNA) is a crucial regulator in the progression of various types of cancers. This article emphasizes the current and future advancements in lncRNAs as potential biomarkers for diagnosing, monitoring, and treating different types of canine tumors, which will benefit veterinary medicine as well as comparative medicine.Abstract Cancer is the leading cause of death in both humans and companion animals. Long non-coding RNA (lncRNA) plays a crucial role in the progression of various types of cancers in humans, involving tumor proliferation, metastasis, angiogenesis, and signaling pathways, and acts as a potential biomarker for diagnosis and targeted treatment. However, research on lncRNAs related to canine tumors is in an early stage. Dogs have long been considered a promising natural model for human disease. This article summarizes the molecular function of lncRNAs as novel biomarkers in various types of canine tumors, providing new insights into canine tumor diagnosis and treatment. Further research on the function and mechanism of lncRNAs is needed, which will benefit both human and veterinary medicine.
Keyword ：
biomarker biomarker canine tumors canine tumors long non-coding RNA long non-coding RNA
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| GB/T 7714 | Zhang, Yan , Wu, Meijin , Zhou, Jiahao et al. Long Non-Coding RNA as a Potential Biomarker for Canine Tumors [J]. | VETERINARY SCIENCES , 2023 , 10 (11) . |
| MLA | Zhang, Yan et al. "Long Non-Coding RNA as a Potential Biomarker for Canine Tumors" . | VETERINARY SCIENCES 10 . 11 (2023) . |
| APA | Zhang, Yan , Wu, Meijin , Zhou, Jiahao , Diao, Hongxiu . Long Non-Coding RNA as a Potential Biomarker for Canine Tumors . | VETERINARY SCIENCES , 2023 , 10 (11) . |
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犬肿瘤性疾病是兽医临床上常发的一种疾病，发病率高且危害大，是造成犬死亡的重要原因之一，尤其是对中老年犬。为了更好地诊断、治疗犬肿瘤性疾病，识别潜在的生物标志物和治疗靶点具有重要意义。非编码RNA(non-coding RNA, ncRNA)是一类不编码蛋白质并具有众多功能的RNA分子，能够通过调节基因转录参与肿瘤细胞发生发展过程，有望成为犬肿瘤性疾病诊断与治疗的生物标志物和潜在治疗靶点。因此，本文针对非编码RNA在犬乳腺肿瘤、黑色素瘤以及骨肉瘤中的诊断价值及应用前景进行综述，以期为犬肿瘤的诊断、预防和治疗提供新思路。
Keyword ：
犬 犬 生物标志物 生物标志物 肿瘤 肿瘤 非编码RNA 非编码RNA
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| GB/T 7714 | 张琰 , 刘佳悦 , 吴梅金 et al. 非编码RNA作为犬肿瘤潜在生物标志物的研究进展 [J]. | 畜牧兽医学报 , 2023 , 54 (06) : 2264-2271 . |
| MLA | 张琰 et al. "非编码RNA作为犬肿瘤潜在生物标志物的研究进展" . | 畜牧兽医学报 54 . 06 (2023) : 2264-2271 . |
| APA | 张琰 , 刘佳悦 , 吴梅金 , 周家豪 , 刁洪秀 . 非编码RNA作为犬肿瘤潜在生物标志物的研究进展 . | 畜牧兽医学报 , 2023 , 54 (06) , 2264-2271 . |
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<正>生物体中的RNA种类繁多，功能复杂，按照是否编码蛋白质可将其分为编码RNA(Coding RNA)和非编码RNA(Non-coding RNA,ncRNA)两大类。随着转录组学的发展和数据的共享，ncRNA的功能研究进入了崭新的阶段，长链非编码RNA(Long non-coding RNA,lncRNA)在ncRNA 中占比最高，且功能最复杂，已成为国际生命科学领域的研究前沿。大量研究证实，lncRNA具有高度的组织特异性，
Keyword ：
lncRNA lncRNA
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| GB/T 7714 | 刘佳悦 , 赵芳莹 , 张琰 et al. lncRNA在奶牛临床常见疾病中的应用 [J]. | 中国兽医杂志 , 2023 , 59 (02) : 98-102 . |
| MLA | 刘佳悦 et al. "lncRNA在奶牛临床常见疾病中的应用" . | 中国兽医杂志 59 . 02 (2023) : 98-102 . |
| APA | 刘佳悦 , 赵芳莹 , 张琰 , 张乐 , 苑金利 , 陈吉龙 et al. lncRNA在奶牛临床常见疾病中的应用 . | 中国兽医杂志 , 2023 , 59 (02) , 98-102 . |
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Canine inflammatory mammary carcinoma (CIMC) is a type of canine malignant mammary tumor with a poor prognosis and high mortality. We transduced firefly luciferase and enhanced green fluorescent protein (EGFP) into CHMp, a CIMC cell line, and established CHMp-Luc-EGFP cells. We investigated the characteristics of this cell line in vitro and in vivo. CHMp-Luc-EGFP was passaged continuously 75 times, with stable expression of luciferase and EGFP. Compared with the wild-type, CHMp-Luc-EGFP had similar proliferation, metastasis, histopathology characteristics, and expression of E-cadherin, N-cadherin, and Ki-67. A tumor-bearing model was established by implantation of CHMp-Luc-EGFP cells, and the dynamic changes of tumors were visualized and quantified using the IVIS imaging system. In summary, the cell line we established could reflect the biological characteristics of CHMp cells, visualize the tumor progression in vivo, and provide a powerful tool for the study of CIMC.
Keyword ：
canine inflammatory mammary carcinoma canine inflammatory mammary carcinoma cell line cell line characteristic characteristic enhanced green fluorescent protein enhanced green fluorescent protein firefly luciferase firefly luciferase tumor-bearing model tumor-bearing model
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| GB/T 7714 | Zhao, Fangying , Li, Xinqiu , Liu, Jiayue et al. Establishment of stable expression of firefly luciferase and EGFP in a canine inflammatory mammary carcinoma cell line and tumor-bearing model in nude mice [J]. | FRONTIERS IN VETERINARY SCIENCE , 2022 , 9 . |
| MLA | Zhao, Fangying et al. "Establishment of stable expression of firefly luciferase and EGFP in a canine inflammatory mammary carcinoma cell line and tumor-bearing model in nude mice" . | FRONTIERS IN VETERINARY SCIENCE 9 (2022) . |
| APA | Zhao, Fangying , Li, Xinqiu , Liu, Jiayue , Zhang, Di , Diao, Hongxiu , Lin, Degui . Establishment of stable expression of firefly luciferase and EGFP in a canine inflammatory mammary carcinoma cell line and tumor-bearing model in nude mice . | FRONTIERS IN VETERINARY SCIENCE , 2022 , 9 . |
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