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学者姓名：聂红毅
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Abstract ：
本发明涉及miR‑281‑x在调控蜜蜂脑部章鱼胺水平、蜜蜂清理行为中的应用。miR‑281‑x定位于1号染色体，含有22个核苷酸，序列为AAGAGAGCUAUCCAUCGACAGU。miR‑281‑x通过靶向Tdc2基因（LOC410948,tyrosine decarboxylase 2）的3’UTR区域，调节蜜蜂脑部章鱼胺的水平，影响蜜蜂对狄斯瓦螨的清理强度。通过过表达或抑制miR‑281‑x的表达水平，可以影响蜜蜂脑部章鱼胺水平，从而降低或提高蜜蜂蜂群中强清理蜜蜂（MS）的比例，有望应用在蜜蜂抗螨育种中减少狄斯瓦螨寄生率达到动态平衡。
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| GB/T 7714 | 苏松坤 , 吕洋 , 欧阳文瑶 et al. miR-281-x在调控蜜蜂脑部章鱼胺水平、蜜蜂清理行为中的应用 : CN202411951303.1[P]. | 2024-12-27 . |
| MLA | 苏松坤 et al. "miR-281-x在调控蜜蜂脑部章鱼胺水平、蜜蜂清理行为中的应用" : CN202411951303.1. | 2024-12-27 . |
| APA | 苏松坤 , 吕洋 , 欧阳文瑶 , 苗刘畅 , 李志国 , 聂红毅 . miR-281-x在调控蜜蜂脑部章鱼胺水平、蜜蜂清理行为中的应用 : CN202411951303.1. | 2024-12-27 . |
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本发明涉及一种鉴别西方蜜蜂蜂群蜂王浆高产性状的SNP标记及其应用。所述SNP标记选自NC_037641.1_10806386、NC_037641.1_10806436。从待测西方蜜蜂蜂群中随机收集成年蜜蜂工蜂个体，提取基因组DNA作为模板进行PCR扩增反应，获得PCR反应产物；对获得的PCR反应产物进行琼脂糖凝胶电泳检测，将出现目的条带的PCR反应液进行测序；对测序结果进行分析，计算待测西方蜜蜂蜂群NC_037641.1_10806386位点或NC_037641.1_10806436位点的基因频率，确定待测西方蜜蜂蜂群是否为蜂王浆高产蜂群。本发明从分子生物学水平提供了与西方蜜蜂蜂王浆高产性状相关的两个SNP标记，且两个SNP标记包含于同一对特异性引物扩增出的目的片段，能够科学、准确、快速地鉴别西方蜜蜂蜂群蜂王浆高产性状，可以大大缩短蜂王浆高产蜜蜂的选育周期，加快其育种速度。
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| GB/T 7714 | 聂红毅 , 傅云熙 , 赖雨 et al. 一种鉴别西方蜜蜂蜂群蜂王浆高产性状的SNP标记及其应用 : CN202411664467.6[P]. | 2024-11-20 . |
| MLA | 聂红毅 et al. "一种鉴别西方蜜蜂蜂群蜂王浆高产性状的SNP标记及其应用" : CN202411664467.6. | 2024-11-20 . |
| APA | 聂红毅 , 傅云熙 , 赖雨 , 苏松坤 , 周诗文 , 王若虹 . 一种鉴别西方蜜蜂蜂群蜂王浆高产性状的SNP标记及其应用 : CN202411664467.6. | 2024-11-20 . |
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本发明涉及一种鉴别西方蜜蜂蜂群王浆高产性状的插入缺失标记NC_037647.1_8674876及其应用。从待测西方蜜蜂蜂群中随机收集成年蜜蜂工蜂个体，提取基因组DNA作为模板，利用NC_037647.1_8674876的引物进行PCR扩增反应，获得PCR反应产物；对获得的PCR反应产物进行琼脂糖凝胶电泳检测，将出现目的条带的PCR反应液进行测序；依据测序结果进行NC_037647.1_8674876位点基因分型，所述基因型为纯合插入型II、纯合缺失型DD或杂合型ID，其中I等位基因表现为14bp插入，D等位基因表现为14bp缺失；计算待测西方蜜蜂蜂群NC_037647.1_8674876位点的等位基因频率，确定待测西方蜜蜂蜂群是否为蜂王浆高产蜂群。本发明能够准确、快速、科学地鉴别西方蜜蜂蜂群蜂王浆高产性状，可以大大缩短蜂王浆高产蜜蜂的选育周期，加快其育种速度。
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| GB/T 7714 | 苏松坤 , 傅云熙 , 赖雨 et al. 一种鉴别西方蜜蜂蜂群蜂王浆高产性状的插入缺失标记NC_037647.1_8674876及其应用 : CN202411663702.8[P]. | 2024-11-20 . |
| MLA | 苏松坤 et al. "一种鉴别西方蜜蜂蜂群蜂王浆高产性状的插入缺失标记NC_037647.1_8674876及其应用" : CN202411663702.8. | 2024-11-20 . |
| APA | 苏松坤 , 傅云熙 , 赖雨 , 聂红毅 , 王若虹 , 周诗文 . 一种鉴别西方蜜蜂蜂群蜂王浆高产性状的插入缺失标记NC_037647.1_8674876及其应用 : CN202411663702.8. | 2024-11-20 . |
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Abstract ：
【目的】分析红背中华蜜蜂Apis cerana cerana色素通路相关基因的表达差异，揭示红背中华蜜蜂色素形成的分子机制。【方法】采用体视显微镜观察刚出房红背中华蜜蜂和黑背中华蜜蜂(正常个体)成年工蜂的体色差异。通过同源比对鉴定中华蜜蜂成年工蜂黑色素代谢通路相关8个基因(PAH,TH,DDC,ebony,tan,aaNAT,yellow-y和laccase 2)、蝶呤通路相关4个基因(GTPCH I,SPR,PTPS和GC-1)、眼色素通路相关2个基因(vermilion和cinnabar)以及尿酸盐转运相关4个基因(BLOS2,HPS5,OK和Varp)的同源基因；运用荧光定量PCR检测上述色素通路相关基因在红背中华蜜蜂和黑背中华蜜蜂成年工蜂胸部背板和腹部体壁中的相对表达量。【结果】红背中华蜜蜂和黑背中华蜜蜂成年工蜂体色差异表现在胸部背板：红背中华蜜蜂胸部背板呈现棕红色，黑背中华蜜蜂胸部背板则为黑色。荧光定量PCR检测结果表明，胸部背板中tan,laccase 2,SPR,vermilion,cinnabar,BLOS2和OK的表达量以及腹部体壁中OK的表达量在红背中华蜜蜂成年工蜂与黑背中华蜜蜂成年工蜂间有显著性差异。【结论】红背中华蜜蜂和黑背中华蜜蜂明显的体色差异位于胸部背板，这种体色分化的现象受到蜜蜂体内黑色素、蝶呤、眼色素通路及尿酸盐转运相关基因共同作用的影响。
Keyword ：
形态观察 形态观察 红背中华蜜蜂 红背中华蜜蜂 胸部背板 胸部背板 色素通路 色素通路 荧光定量PCR 荧光定量PCR
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| GB/T 7714 | 王若虹 , 杨振慧 , 周诗文 et al. 色素通路相关基因在红背和黑背中华蜜蜂成年工蜂背板中的表达模式分析 [J]. | 昆虫学报 , 2024 , 67 (01) : 9-17 . |
| MLA | 王若虹 et al. "色素通路相关基因在红背和黑背中华蜜蜂成年工蜂背板中的表达模式分析" . | 昆虫学报 67 . 01 (2024) : 9-17 . |
| APA | 王若虹 , 杨振慧 , 周诗文 , 吴雨珈 , 李秋方 , 梁立强 et al. 色素通路相关基因在红背和黑背中华蜜蜂成年工蜂背板中的表达模式分析 . | 昆虫学报 , 2024 , 67 (01) , 9-17 . |
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【目的】基于抗白垩病相关单核苷酸多态性(single nucleotide polymorphism, SNP)位点C2587245T在意大利蜜蜂Apis mellifera ligustica雄蜂幼虫中白垩病抗性检验，验证该位点遗传稳定性和抗病性，为分子标记辅助育种在育种生产中直接应用提供技术支持。【方法】通过白垩病虫尸，在实验室条件下制备白垩真菌孢子悬液，通过形态学和分子生物学方法鉴定蜜蜂球囊菌Ascosphaera apis。使用无伤害方法提取意大利蜜蜂蜂蜂王DNA筛选出SNP位点C2587245T为C/C和T/T基因型的蜂王，并培育C/C和T/T基因型处女王，用二氧化碳刺激其产出雄蜂卵，选择C和T基因型2日龄意大利蜜雄蜂幼虫进行实验室培养，3日龄龄雄蜂幼虫接种5×10~4个孢子/μL的蜜蜂球囊菌悬液10 d,观测接种蜜蜂球囊菌后的C基因型和T基因型雄蜂幼虫的生长情况和存活率。【结果】通过无伤害提取DNA的方法可以在不影响意大利蜜蜂雄蜂幼虫正常生活的条件下提取出高质量的DNA;用本研究的饲养方法可以保证意大利蜜蜂雄蜂幼虫在实验室条件的正常生长发育。C基因型和T基因型意大利蜜蜂雄蜂3日龄幼虫接种蜜蜂球囊菌后在发病时间和发病症状等方面都有明显差异，C基因型雄蜂幼虫在表现疾病典型症状的时间上比T基因型雄蜂幼虫晚大约2 d;在接种蜜蜂球囊菌后6 d时两种基因型雄蜂幼虫表现出的症状差异最为明显。【结论】雄蜂由于其纯合单倍体的生物学特性，更方便验证SNP位点C2587245T纯合的抗病作用。SNP位点C2587245T为C基因型雄蜂幼虫具有良好的抗白垩病能力，并且SNP位点C2587245T具有稳定的遗传性，这些研究结果可为在蜜蜂抗病育种领域提供一种可靠的分子辅助标记，为后续雄蜂转录组测序、寻找雄蜂和工蜂在免疫相关基因表达方面的差异，尝试找到SNP位点C2587245T对白垩病所特有的抗病机制提供基础。
Keyword ：
单核苷酸多态性 单核苷酸多态性 意大利蜜蜂 意大利蜜蜂 白垩病 白垩病 白垩病抗性 白垩病抗性 蜜蜂球囊菌 蜜蜂球囊菌 雄蜂 雄蜂
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| GB/T 7714 | 唐韶晗 , 耿龙 , 武尊 et al. 抗白垩病相关SNP位点C2587245T在意大利蜜蜂雄蜂幼虫中的抗性鉴定 [J]. | 昆虫学报 , 2024 , 67 (01) : 29-37 . |
| MLA | 唐韶晗 et al. "抗白垩病相关SNP位点C2587245T在意大利蜜蜂雄蜂幼虫中的抗性鉴定" . | 昆虫学报 67 . 01 (2024) : 29-37 . |
| APA | 唐韶晗 , 耿龙 , 武尊 , 曾照阳 , 王子函 , 梁立强 et al. 抗白垩病相关SNP位点C2587245T在意大利蜜蜂雄蜂幼虫中的抗性鉴定 . | 昆虫学报 , 2024 , 67 (01) , 29-37 . |
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Stingers, evolved from ovipositors, are an important defense organ for the Apidae, Vespidae, and Formicidae species. However, the molecular mechanism of stinger development remains unclear. Here, we show that the earliest time point for the appearance of stingers in Apis mellifera is at the 1-day-old worker pupal stage based on morphological observations and anatomy from the pre-pupal to adult stages. To discover the genes related to stinger development, we first comprehensively compared the stinger transcriptome at different stages and screened 1282, 186, and 166 highly expressed genes in the stingers of 1- and 5-day-old worker pupae and newly emerged worker bees (NEBs), respectively, then identified 25 DEGs involved in the early stage of stinger development. We found that Dll was a key candidate gene in the early development of A. mellifera stingers by combining analyses of the protein-protein interaction network and spatiotemporal expression patterns. An RNAi experiment showed that about 20% of individuals exhibited tip bending in the piercing parts of their stingers in the Dll-dsRNA-treated group, with the morphology presenting as side-side or front-back tip bending. This indicates that Dll plays a vital role in the early development of A. mellifera stingers. Together, our study provides insight into the molecular mechanism of Hymenoptera stinger development and an inspiration for the molecular breeding of gentle honeybee species with stinger abnormalities.
Keyword ：
Apis mellifera Apis mellifera Dll Dll highly expressed genes highly expressed genes morphological observation morphological observation RNAi RNAi stinger stinger tip bending tip bending transcriptome transcriptome
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| GB/T 7714 | Zhou, Shiwen , Zhang, Juan , Yang, Zhenhui et al. Transcriptomic Analysis of Genes Associated with Stinger Development at Different Life Stages of Apis mellifera [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (19) . |
| MLA | Zhou, Shiwen et al. "Transcriptomic Analysis of Genes Associated with Stinger Development at Different Life Stages of Apis mellifera" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25 . 19 (2024) . |
| APA | Zhou, Shiwen , Zhang, Juan , Yang, Zhenhui , Fu, Yunxi , Lai, Yu , Xu, Xueling et al. Transcriptomic Analysis of Genes Associated with Stinger Development at Different Life Stages of Apis mellifera . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (19) . |
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The appetite of honeybees for food is crucial to their survival and reproduction, as they sustain their entire colony by collecting pollen and nectar for nutrients. Dopamine, an important neurotransmitter, regulates appetite and satiety. However, how dopamine regulates honeybee foraging behavior remains unexplored. In this study, we investigated dopamine expression in 23-day-old Apis mellifera under different food-wanting conditions and identified differentially expressed genes (DEGs) in the brains of honeybees using RNA sequencing technology. We showed that dopamine levels in honeybees starved for 2 h were higher than those sated after 2 h of starvation. RNA-seq results revealed there were differences in the expression of cytochrome P450-dependent monooxygenase (CYP9Q1) in honeybees, which regulated the sucrose sensitivity of honeybees under different intake states. Furthermore, CYP9Q1 targeted the expression of the insulin receptor substrate (IRS) to promote dopamine synthesis. Our findings emphasize the relationship between dopamine and honeybees' desire for food at the molecular level, providing a reference for further exploring the mechanism of food wanting.
Keyword ：
Cytochrome P450 Cytochrome P450 dopamine dopamine food wanting food wanting honeybees honeybees RNA-seq RNA-seq
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| GB/T 7714 | Xu, Xue-Ling , Geng, Long , Zeng, Zhao-Yang et al. CYP9Q1 Modulates Dopamine to Increase Sugar Responsiveness in Honeybees (Apis mellifera) [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (24) . |
| MLA | Xu, Xue-Ling et al. "CYP9Q1 Modulates Dopamine to Increase Sugar Responsiveness in Honeybees (Apis mellifera)" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25 . 24 (2024) . |
| APA | Xu, Xue-Ling , Geng, Long , Zeng, Zhao-Yang , Wu, Zun , Li, Lin-Feng , Tang, Shao-Han et al. CYP9Q1 Modulates Dopamine to Increase Sugar Responsiveness in Honeybees (Apis mellifera) . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (24) . |
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Royal jelly (RJ), a substance secreted by the hypopharyngeal and mandibular glands of nurse worker bees, is widely used in medical products, dietary supplements, health foods, and cosmetics, owing to its potential health benefits. An understanding of secretory glands, such as the hypopharyngeal glands (HGs), and their interactions are necessary for RJ secretion. The RJ production cycle should be expanded for more effective production, which requires additional experience and training. However, the association between RJ secretion and HGs levels remains unclear. To close this gap, we conducted this review to investigate the most recent advancements in HGs and RJ secretion in Apis mellifera. These include techniques for enhancing RJ production, the morphology of HGs, RJ biological and effectiveness in the treatment of diseases, a comparison of the secretion of RJ signaling pathways in honey bees, associated genes, the proteins of HGs, and elements that could affect the secretion of HGs and the mechanism of RJ secretion. In order to help bee products strategy, our review may be valuable for a better understanding of the association between HGs and RJ secretion.
Keyword ：
Apis mellifera Apis mellifera hypopharyngeal glands hypopharyngeal glands production production royal jelly royal jelly secretion secretion
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| GB/T 7714 | Hassanyar, Aqai Kalan , Huang, Jingnan , Nie, Hongyi et al. The association between the hypopharyngeal glands and the molecular mechanism which honey bees secrete royal jelly [J]. | JOURNAL OF APICULTURAL RESEARCH , 2023 , 64 (1) : 281-296 . |
| MLA | Hassanyar, Aqai Kalan et al. "The association between the hypopharyngeal glands and the molecular mechanism which honey bees secrete royal jelly" . | JOURNAL OF APICULTURAL RESEARCH 64 . 1 (2023) : 281-296 . |
| APA | Hassanyar, Aqai Kalan , Huang, Jingnan , Nie, Hongyi , Li, Zhiguo , Hussain, Mubasher , Rizwan, Muhammad et al. The association between the hypopharyngeal glands and the molecular mechanism which honey bees secrete royal jelly . | JOURNAL OF APICULTURAL RESEARCH , 2023 , 64 (1) , 281-296 . |
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One of the brain's primary functions is remembering and learning information related to food and odour. Since biogenic amines were discovered in invertebrates and vertebrate, dopamine is considered a key modulator and neurotransmitter in honeybees' olfactory learning. Dopamine (DA) is significant in rewarding prediction, learning, invigorating social behavior, and motivation. Here we examined the effect of dopamine in the olfactory learning behavior of honeybees. We used the same age (14-days old) honeybees, Apis mellifera and Apis cerana to evaluate the proboscis extension response and characterized brain dopamine's effect on olfactory learning behavior. Both species were individually trained by performing three learning trials with sucrose solution and odor 1-Hexanol. High-performance liquid chromatography (HPLC) determined the brain dopamine level using electrochemical detection. Our findings showed that Apis mellifera learned better and had higher brain dopamine levels than Apis cerana. Thus, we show that dopamine acts as an essential neurotransmitter and modulator of motivation and influences honeybee cognition.
Keyword ：
Biogenic amine Biogenic amine dopamine dopamine olfactory conditioning olfactory conditioning proboscis extension response proboscis extension response social insect social insect
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| GB/T 7714 | Raza, Muhammad Fahad , Li, Zhiguo , Nie, Hongyi et al. Insights into the role of dopamine in olfactory learning behavior of honeybee [J]. | KUWAIT JOURNAL OF SCIENCE , 2023 , 50 (1) . |
| MLA | Raza, Muhammad Fahad et al. "Insights into the role of dopamine in olfactory learning behavior of honeybee" . | KUWAIT JOURNAL OF SCIENCE 50 . 1 (2023) . |
| APA | Raza, Muhammad Fahad , Li, Zhiguo , Nie, Hongyi , Su, Songkun . Insights into the role of dopamine in olfactory learning behavior of honeybee . | KUWAIT JOURNAL OF SCIENCE , 2023 , 50 (1) . |
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为了筛选西方蜜蜂采集蜂上颚腺中高表达基因,本研究基于已测序的工蜂上颚腺转录组数据,筛选到22个DEGs在采集蜂上颚腺中高表达,这些DEGs主要富集在胆固醇代谢、半乳糖代谢、淀粉和蔗糖代谢等方面。8个DEGs的表达模式与转录组数据的表达模式一致;2个关键DEGs Amp5cs和CYP9Q3在采集蜂中表达量最高;Amp5cs在采集蜂腹、胸、上颚腺和触角中高量表达,P450 9e2在采集蜂触角和足中表达量显著高于在其他组织中的。这些DEGs可能主要参与采集蜂上颚腺生理发育以及能量供应、外源性物质解毒、花蜜转化等代谢通路,进而影响蜜蜂的采集行为。
Keyword ：
Δ-1-吡咯啉-5-羧酸合成酶 Δ-1-吡咯啉-5-羧酸合成酶 上颚腺 上颚腺 细胞色素P450 9e2 细胞色素P450 9e2 西方蜜蜂 西方蜜蜂 采集行为 采集行为
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| GB/T 7714 | 李秋方 , 高艳 , 梁立强 et al. 采集蜂上颚腺中高表达基因的筛选与表达分析 [C] //2023年中国（浙江·江山）蜂业博览会暨全国蜂产品市场信息交流会 . 2023 . |
| MLA | 李秋方 et al. "采集蜂上颚腺中高表达基因的筛选与表达分析" 2023年中国（浙江·江山）蜂业博览会暨全国蜂产品市场信息交流会 . (2023) . |
| APA | 李秋方 , 高艳 , 梁立强 , 李正汉卿 , 朱雅楠 , 张娟 et al. 采集蜂上颚腺中高表达基因的筛选与表达分析 2023年中国（浙江·江山）蜂业博览会暨全国蜂产品市场信息交流会 . (2023) . |
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