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学者姓名：石振华
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Abstract ：
本发明涉及植物电响应技术领域，具体涉及一种表征植物根系电双趋性的方法及其应用，在长方体水槽的两侧平行插入两块金属板，两块所述金属板分别与直流电源的正极和负极相连，在水槽中加入培养基质组成一个闭合回路，将种子置于海绵上并用双层纱布覆盖，随后将海绵放入水槽的培养基质中对种子进行培养；通过调节施加在培养基质两侧的电压强度和流经培养基质的电流强度，使种子长出的根系向阴极和阳极方向生长，以此表征植物根系电双趋性。本发明方法简单，操作易行、观察方便，能够清楚表征植物根系的电双趋性，且能够反应电双趋性时的电响应条件和电响应特征，具有广阔的应用前景。
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| GB/T 7714 | 石振华 , 王灵敏 , 张英蓉 . 一种表征植物根系电双趋性的方法及其应用 : CN202510158323.1[P]. | 2025-02-13 . |
| MLA | 石振华 等. "一种表征植物根系电双趋性的方法及其应用" : CN202510158323.1. | 2025-02-13 . |
| APA | 石振华 , 王灵敏 , 张英蓉 . 一种表征植物根系电双趋性的方法及其应用 : CN202510158323.1. | 2025-02-13 . |
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The fact that all cell organisms have electron transport chains and that all cell organisms need to obtain electron donors and electron acceptors from the environment to survive inspired that all cell organisms should have innate bidirectional tropism toward both electron donors and electron acceptors. Here we confirmed this hypothesis in wheat, that is, under a certain voltage condition, the smaller the current in the culture medium, the more roots grew toward the cathode (electron donor). As the current increases, more and more roots grew toward the anode (electron acceptor). More importantly, although the root growth direction was opposite under certain voltage and current conditions, the growth rate of the plant was increased. Moreover, LC-MS/MS-based metabolomics analysis showed that metabolites involved in energy metabolism (e.g., glucose-6-phosphat, fumaric acid, and L-malic acid) and secondary metabolism (e.g., 4-methoxycinnamic acid, caffeine, and coumarin) are closely related to this behavior. The relationship between bidirectional electrotropism and energy metabolism was further confirmed by examining the gene expression level of enzymes involved in both the glycolysis pathway and tricarboxylic acid cycle, the activity of antioxidant enzymes, the level of cellular ATP, and the inhibition of malonic acid. This study is the first report of bidirectional electrotropism of living organisms, and as the behavior of bidirectional electrotropism is based on the common of all cellular organisms, the ancestral role of this behavior in the origin and evolution of life is desirable.
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| GB/T 7714 | Shi, Zhenhua , Wang, Lingmin , Zhang, Yingrong et al. Bidirectional electrotropism of wheat root [J]. | PLANT JOURNAL , 2025 , 122 (6) . |
| MLA | Shi, Zhenhua et al. "Bidirectional electrotropism of wheat root" . | PLANT JOURNAL 122 . 6 (2025) . |
| APA | Shi, Zhenhua , Wang, Lingmin , Zhang, Yingrong , Yu, Zhen . Bidirectional electrotropism of wheat root . | PLANT JOURNAL , 2025 , 122 (6) . |
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Abstract ：
本发明涉及植物电响应技术领域，具体涉及一种调控植物能量代谢的方法及其应用，本发明利用植物的电双趋性调控植物能量代谢，实现了植物能量代谢的定向调控，提高植物能量体用效率和生长发育，解决了现有技术无法定向调节植物能量代谢的问题。
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| GB/T 7714 | 石振华 , 王灵敏 , 张英蓉 . 一种调控植物能量代谢的方法及其应用 : CN202510158360.2[P]. | 2025-02-13 . |
| MLA | 石振华 et al. "一种调控植物能量代谢的方法及其应用" : CN202510158360.2. | 2025-02-13 . |
| APA | 石振华 , 王灵敏 , 张英蓉 . 一种调控植物能量代谢的方法及其应用 : CN202510158360.2. | 2025-02-13 . |
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Triclosan (TCS) can influence energy metabolism and is a potential obesogen. However, its underlying mechanisms remain largely unknown. This study investigated how low-dose TCS exposure (0.5 mg/kg/day) disrupts energy metabolism in Sprague-Dawley rats. TCS increased body weight, visceral fat, liver lipid accumulation, and serum triglyceride levels. It also promoted hyperphagia by altering hypothalamic appetite regulation, activating orexigenic neuropeptide Y neurons and suppressing anorexigenic pro-opiomelanocortin neurons. Furthermore, TCS may reduce brown adipose tissue thermogenesis, as indicated by decreased mitochondrial uncoupling protein 1 and tyrosine hydroxylase. These metabolic effects were blocked by subdiaphragmatic vagotomy, confirming gut-brain neural circuit involvement. Mechanistically, TCS reduced gut microbial diversity and butyrate levels. Crucially, both fecal microbiota transplantation from control rats and butyrate supplementation reversed TCS-induced metabolic dysregulation. These findings reveal that TCS-induced gut dysbiosis and butyrate reduction as key drivers of metabolic disturbances and offer insights into the role of environmental chemicals in obesity and potential therapeutic strategies targeting the gut microbiota and butyrate.
Keyword ：
Brown adipose tissue Brown adipose tissue Butyrate Butyrate Gut microbes Gut microbes Obesity Obesity Triclosan Triclosan
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| GB/T 7714 | Yu, Zhen , Wu, Jushuang , Han, Junyong et al. Triclosan induced obesity via gut microbiota dysbiosis and butyrate reduction [J]. | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 2025 , 305 . |
| MLA | Yu, Zhen et al. "Triclosan induced obesity via gut microbiota dysbiosis and butyrate reduction" . | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 305 (2025) . |
| APA | Yu, Zhen , Wu, Jushuang , Han, Junyong , Wang, Lingmin , Lu, Rongmei , Chen, Jinyan et al. Triclosan induced obesity via gut microbiota dysbiosis and butyrate reduction . | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 2025 , 305 . |
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4-Methylcytosine (4mC) and 6-methyladenine (6mA) are the most prevalent types of DNA modifications in prokaryotes. However, whether there is crosstalk between 4mC and 6mA remain unknown. Here, methylomes and transcriptomes of Geobacter sulfurreducens exposed to different intensities of extremely low frequency electromagnetic fields (ELF-EMF) were investigated. Results showed that the second adenine of all the 50-GTACAG-30 0-GTACAG-3 0 motif was modified to 6mA (M-6mA). For the other 6mA (O-6mA), the variation in their distance from the neighboring M-6mA increased with the intensity of ELF-EMF. Moreover, cytosine adjacent to O-6mA has a much higher probability of being modified to 4mC than cytosine adjacent to M-6mA, and the closer an unmodified cytosine is to 4mC, the higher the probability that the cytosine will be modified to 4mC. Furthermore, there was no significant correlation between DNA methylation and gene expression regulation. These results suggest a reference signal that goes from M-6mA to O-6mA to 4mC.
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| GB/T 7714 | Shi, Zhenhua , Zhang, Yingrong , Chen, Wanqiu et al. Crosstalk between 6-methyladenine and 4-methylcytosine in Geobacter sulfurreducens exposed to extremely low-frequency electromagnetic field [J]. | ISCIENCE , 2024 , 27 (9) . |
| MLA | Shi, Zhenhua et al. "Crosstalk between 6-methyladenine and 4-methylcytosine in Geobacter sulfurreducens exposed to extremely low-frequency electromagnetic field" . | ISCIENCE 27 . 9 (2024) . |
| APA | Shi, Zhenhua , Zhang, Yingrong , Chen, Wanqiu , Yu, Zhen . Crosstalk between 6-methyladenine and 4-methylcytosine in Geobacter sulfurreducens exposed to extremely low-frequency electromagnetic field . | ISCIENCE , 2024 , 27 (9) . |
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Numerous works have reported that extremely low frequency electromagnetic fields (ELF-EMFs) were associated with human health; however, little is known about their effects on the occurrence of agricultural diseases. In this study, Magnaporthe oryzae was used as a model organism, and its pathogenicity under 50 Hz, 3 mT ELF-EMF was studied. Our results showed that the pathogenicity, growth rate, and conidia generation of M. oryzae were enhanced under ELF-EMF exposure. In addition, M. oryzae exposed to ELF-EMF showed enhanced tolerance to cell wall-perturbing agents sodium lauryl sulphate, and increased expression of cell wall integrity-related genes, including RAC1, CDC42, RHO2, and NOX2. In addition, the level of reactive oxygen species (ROS) and the expression level of ROS scavenger system-related gene MoAP1 increased in ELF-EMF-exposed samples, whereas the total antioxidant capacity and the activities of superoxide dismutase and catalase did not change. Results of our study demonstrated that exposure to 50 Hz, 3 mT ELF-EMF enhanced the infection ability of M. oryzae, which present new important challenges for understanding the effect of ELF-EMF exposure on farmland ecology, especially on agricultural diseases.
Keyword ：
Agricultural disease Agricultural disease ELF-EMF ELF-EMF Magnaporthe oryzae Magnaporthe oryzae MoAP1 MoAP1 Pathogenicity Pathogenicity
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| GB/T 7714 | Chen, Wanqiu , Zhang, Yingrong , Shi, Zhenhua . Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 870 . |
| MLA | Chen, Wanqiu et al. "Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae" . | SCIENCE OF THE TOTAL ENVIRONMENT 870 (2023) . |
| APA | Chen, Wanqiu , Zhang, Yingrong , Shi, Zhenhua . Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 870 . |
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Single-molecule real-time DNA sequencing revealed that 4-methylcytosine (m4C) commonly exists in bacterial genomes. In this work, samples with different m4C methylation patterns were studied. Results reveal that m4C modification is a biochemical reaction with distance effect, and its distribution follows the power function in the positive, negative, and double strands of genomic DNA sequences of Geobacter sulfurreducens. Furthermore, the value of regression coefficient in the fitting formula for double strands was the sum of those in the fitting formulae for positive and negative strands. Meanwhile, the value of exponent coefficient was the average, implicating an interesting mathematical phenomenon about power function. Considering the potent role of m4C in gene expression and the present results being obtained from the same genomic DNA sequence, this work suggests that the patterns of m4C distribution may be served as a signal for G. sulfurreducens to rapidly identify the genes to respond to environmental stresses or signals. This study opens a new avenue to extend our knowledge about the modification mechanisms and the epigenetic information of m4C modification in prokaryotes. (c) 2021 Elsevier Inc. All rights reserved.
Keyword ：
4-Methylcytosine distribution 4-Methylcytosine distribution DNA methylation DNA methylation Geobacter sulfurreducens Geobacter sulfurreducens Power function Power function
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| GB/T 7714 | Shi, Zhenhua , Yu, Zhen , Chen, Wanqiu . 4-Methylcytosine distribution follows the power function in Geobacter sulfurreducens genome [J]. | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 547 : 65-68 . |
| MLA | Shi, Zhenhua et al. "4-Methylcytosine distribution follows the power function in Geobacter sulfurreducens genome" . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 547 (2021) : 65-68 . |
| APA | Shi, Zhenhua , Yu, Zhen , Chen, Wanqiu . 4-Methylcytosine distribution follows the power function in Geobacter sulfurreducens genome . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 547 , 65-68 . |
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Di(2-ethylhexyl) phthalate (DEHP) is one of the most widespread environmental contaminants worldwide because of its massive production, extensive use in common products, and liability to leach from products. This study investigated the mechanisms of DEHP mediated alteration of lipid metabolism. Rats were treated with 0.5 mg kg(-1) d(-1) of DEHP for 23 weeks. Results showed that the treatment induced cholesterol imbalance. Further fecal transplantation experiments corroborated the involvement of gut microbiota in DEHP-induced cholesterol imbalance. In addition, 16S rRNA gene sequencing analysis of cecal contents showed that DEHP disrupted the gut microbiota diversity in rats and increased the ratio of Firmicutes to Bacteroidetes. Further cecal metabolomic analyses, bile salt hydrolase enzyme activity, and gene expression examination revealed that chronic DEHP exposure generated a bile acid profile in the gut that is a more potent activator of farnesoid X receptor (FXR). The activation of FXR in the gut induced the expression of fibroblast growth factor 15, which subsequently suppressed cytochrome P450 family 7 subfamily A member 1 in the liver and bile acid synthesis. These results suggest that DEHP might induce cholesterol imbalance by regulating bile acid metabolism via the remodeling of the gut microbiota. (C) 2020 Elsevier Ltd. All rights reserved.
Keyword ：
Bile acid metabolism Bile acid metabolism Chronic exposure Chronic exposure DEHP DEHP Gut microbiota Gut microbiota Lipid metabolism Lipid metabolism
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| GB/T 7714 | Yu, Zhen , Shi, Zhenhua , Zheng, Zeyu et al. DEHP induce cholesterol imbalance via disturbing bile acid metabolism by altering the composition of gut microbiota in rats [J]. | CHEMOSPHERE , 2021 , 263 . |
| MLA | Yu, Zhen et al. "DEHP induce cholesterol imbalance via disturbing bile acid metabolism by altering the composition of gut microbiota in rats" . | CHEMOSPHERE 263 (2021) . |
| APA | Yu, Zhen , Shi, Zhenhua , Zheng, Zeyu , Han, Junyong , Yang, Wencong , Lu, Rongmei et al. DEHP induce cholesterol imbalance via disturbing bile acid metabolism by altering the composition of gut microbiota in rats . | CHEMOSPHERE , 2021 , 263 . |
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本发明提供一种收集纤维素降解菌菌体的方法，属于环境微生物技术领域。具体步骤是：首先利用LB液体培养基扩大培养纤维素降解细菌；通过离心去除培养基，用无菌水将菌体吹吸均匀后作为接种液；无菌滤纸片蘸取菌液后平铺于CMC固体培养板上，37℃培养72 h；通过收集滤纸片即可得用于检测纤维素降解细菌纤维素酶活性所需样品。该方法操作简单，结果稳定、省时且可靠，在对纤维素降解菌纤维素酶活性表征方面具有广泛的应用价值。
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| GB/T 7714 | 石振华 , 张琪 , 周顺桂 . 一种收集纤维素降解菌菌体的方法 : CN202010654253.6[P]. | 2020-07-09 . |
| MLA | 石振华 et al. "一种收集纤维素降解菌菌体的方法" : CN202010654253.6. | 2020-07-09 . |
| APA | 石振华 , 张琪 , 周顺桂 . 一种收集纤维素降解菌菌体的方法 : CN202010654253.6. | 2020-07-09 . |
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宋朝苏轼在其《题西林壁》中所言：不识庐山真面目，只缘身在此山中。意在表明，人们所处的位置不同，看问题的角度也就不同，对客观事物的认识难免存在一定的片面性。只有选择合适的认识角度，摆脱主观成见，超越狭小的视角范围，才能获取对事物真相和全貌相对更客观、更准确的认识。 1.1生物起源与进化的认识现状及存在问题 ... ... ...
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| GB/T 7714 | 石振华 . 隐藏的动力：生物在自然界中的价值 [M] . 汕头 : . 汕头大学出版社 , . 2020-12-01 . |
| MLA | 石振华 . "隐藏的动力：生物在自然界中的价值" . 汕头 : . 汕头大学出版社 , . 2020-12-01 . |
| APA | 石振华 . 隐藏的动力：生物在自然界中的价值 . 汕头 : . 汕头大学出版社 , . 2020-12-01 . |
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