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学者姓名：王杰
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Abstract ：
Enhancing the oil or protein content of soybean, a major crop for oil and protein production is highly desirable. GmSWEET10a encodes a sugar transporter that is strongly selected during domestication and breeding, increasing seed size and oil content. GmSWEET10b is functionally similar to GmSWEET10a, yet has not been artificially selected. Here, AlphaFold is used to find that C-terminal variants of GmSWEET10a can endow enhanced or reduced transport activity. Guided by AlphaFold, the functionality is improved for GmSWEET10a in terms of oil content through gene editing. Furthermore, novel GmSWEET10b haplotypes possessing strengthened or weakened sugar-transport capabilities that are absent in nature are engineered. Consequently, soybean oil content or protein content in independent GmSWEET10b gene-edited lines during multi-year and multi-site field trials is consistently increased, without negatively affecting yield. The study demonstrates that the combination of AlphaFold-guided protein design and gene editing has the potential to generate novel beneficial alleles, which can optimize protein function in the context of crop breeding.
Keyword ：
AlphaFold AlphaFold CRISPR-Cas9 CRISPR-Cas9 gene editing gene editing oil content oil content precision breeding precision breeding soybean soybean SWEET transporter SWEET transporter
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| GB/T 7714 | Wang, Jie , Zhang, Li , Wang, Shoudong et al. AlphaFold-Guided Bespoke Gene Editing Enhances Field-Grown Soybean Oil Contents [J]. | ADVANCED SCIENCE , 2025 , 12 (23) . |
| MLA | Wang, Jie et al. "AlphaFold-Guided Bespoke Gene Editing Enhances Field-Grown Soybean Oil Contents" . | ADVANCED SCIENCE 12 . 23 (2025) . |
| APA | Wang, Jie , Zhang, Li , Wang, Shoudong , Wang, Xin , Li, Suning , Gong, Pingping et al. AlphaFold-Guided Bespoke Gene Editing Enhances Field-Grown Soybean Oil Contents . | ADVANCED SCIENCE , 2025 , 12 (23) . |
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Enhancing nitrogen-use efficiency is essential for boosting crop yields and advancing sustainable agriculture, particularly in the absence of synthetic fertilizers. Despite the inherent nitrogen-fixation capacity of the staple legume crop soybean (Glycine max) by symbiotic rhizobia, improving nitrogen use has been challenging. Here, a role for the auxin-efflux transporters PIN3a and PIN3b in soybean nitrate acquisition is uncovered. PIN3a/b localizes to the plasma membrane, and high environmental nitrate induces PIN3a degradation and its accumulation at cell junctions. Disrupting PIN3 homologs results in auxin over-accumulation, impairs pavement-cell polarity, and enhances signaling via the transcription factors ARF and STF3/4. These transcription factors separately bind to and activate the NPF2.13 promoter, thereby strengthening nitrate uptake. pin3ab and pin3abd mutants have enhanced nitrate acquisition and resistant to high nitrate on pavement-cell growth. The elevated nitrogen accumulation translates to higher oil contents in pin3ab mutant seeds in an elite cultivar background across multiple years and field locations. The findings shed light on the regulation of nitrate uptake in crop-plant development and demonstrate the unexpected potential of manipulating auxin transporters to enhance soybean nitrogen-use efficiency and agronomic performance.
Keyword ：
auxin auxin leaf development leaf development nitrate nitrate PIN3 PIN3 seed oil seed oil soybean soybean
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| GB/T 7714 | Xu, Huifang , Huang, Shiyu , Wang, Jie et al. Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits [J]. | ADVANCED SCIENCE , 2025 . |
| MLA | Xu, Huifang et al. "Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits" . | ADVANCED SCIENCE (2025) . |
| APA | Xu, Huifang , Huang, Shiyu , Wang, Jie , Wang, Tian , Han, Qingqing , Wu, Kexin et al. Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits . | ADVANCED SCIENCE , 2025 . |
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Symbiotic nitrogen fixation in legume nodules requires substantial energy investment from host plants, and soybean (Glycine max (L.) supernodulation mutants show stunting and yield penalties due to overconsumption of carbon sources. We obtained soybean mutants differing in their nodulation ability, among which rhizobially induced cle1a/2a (ric1a/2a) has a moderate increase in nodule number, balanced carbon allocation, and enhanced carbon and nitrogen acquisition. In multi-year and multi-site field trials in China, two ric1a/2a lines had improved grain yield, protein content and sustained oil content, demonstrating that gene editing towards optimal nodulation improves soybean yield and quality. This study shows that optimizing soybean nodulation, rather than supernodulation, through editing improves N and C assimilation by balancing source-sink relationships. As a result, soybean yield and protein content are simultaneously increased in field conditions.
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| GB/T 7714 | Zhong, Xiangbin , Wang, Jie , Shi, Xiaolei et al. Genetically optimizing soybean nodulation improves yield and protein content [J]. | NATURE PLANTS , 2024 , 10 (5) . |
| MLA | Zhong, Xiangbin et al. "Genetically optimizing soybean nodulation improves yield and protein content" . | NATURE PLANTS 10 . 5 (2024) . |
| APA | Zhong, Xiangbin , Wang, Jie , Shi, Xiaolei , Bai, Mengyan , Yuan, Cuicui , Cai, Chenlin et al. Genetically optimizing soybean nodulation improves yield and protein content . | NATURE PLANTS , 2024 , 10 (5) . |
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Soybean is one of the most versatile crops for oil production, human diets, and feedstocks. The vegetative biomass of soybean is an important determinant of seed yield and is crucial for the forage usages. However, the genetic control of soybean biomass is not well explained. In this work, we used a soybean germplasm population, including 231 improved cultivars, 207 landraces, and 121 wild soybeans, to investigate the genetic basis of biomass accumulation of soybean plants at the V6 stage. We found that biomass-related traits, including NDW (nodule dry weight), RDW (root dry weight), SDW (shoot dry weight), and TDW (total dry weight), were domesticated during soybean evolution. In total, 10 loci, encompassing 47 putative candidate genes, were detected for all biomass-related traits by a genome-wide association study. Among these loci, seven domestication sweeps and six improvement sweeps were identified. Glyma.05G047900, a purple acid phosphatase, was a strong candidate gene to improve biomass for future soybean breeding. This study provided new insights into the genetic basis of biomass accumulation during soybean evolution.
Keyword ：
Biomass Biomass Genetic basis Genetic basis GWAS GWAS Soybean evolution Soybean evolution Symbiotic nitrogen fixation Symbiotic nitrogen fixation
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| GB/T 7714 | Wang, Xin , Zhou, Shaodong , Wang, Jie et al. Genome-wide association study for biomass accumulation traits in soybean [J]. | MOLECULAR BREEDING , 2023 , 43 (5) . |
| MLA | Wang, Xin et al. "Genome-wide association study for biomass accumulation traits in soybean" . | MOLECULAR BREEDING 43 . 5 (2023) . |
| APA | Wang, Xin , Zhou, Shaodong , Wang, Jie , Lin, Wenxin , Yao, Xiaolei , Su, Jiaqing et al. Genome-wide association study for biomass accumulation traits in soybean . | MOLECULAR BREEDING , 2023 , 43 (5) . |
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Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding. Nevertheless, a significant portion of soybean cultivars are still pod shattering-susceptible, limiting their regional and climatic adaptabilities. Here we performed genetic diagnosis on the shattering-susceptible trait of a national registered cultivar, Huachun6 (HC6), and found that HC6 carries the susceptible genotype of a candidate Pod dehiscence 1 (PDH1) gene, which exists in a significant portion of soybean cultivars. We next performed genome editing on PDH1 gene by clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9). In T2 progenies, several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits. The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma. Our work demonstrated that precision breeding by genome editing on PDH1 holds great potential for precisely improving pod shattering resistance and adaptability of soybean cultivars. © 2022, The Author(s).
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| GB/T 7714 | Guan, Yuefeng , Yan, Long , Song, Shikui et al. Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9 [J]. | aBIOTECH , 2022 , 3 (2) : 110-114 . |
| MLA | Guan, Yuefeng et al. "Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9" . | aBIOTECH 3 . 2 (2022) : 110-114 . |
| APA | Guan, Yuefeng , Yan, Long , Song, Shikui , Yao, Xiaolei , Zhou, Shaodong , Bai, Mengyan et al. Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9 . | aBIOTECH , 2022 , 3 (2) , 110-114 . |
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Beany flavor induced by three lipoxygenases (LOXs, including LOX1, LOX2, and LOX3) restricts human consumption of soybean. It is desirable to generate lipoxygenase-free new mutant lines to improve the eating quality of soybean oil and protein products. In this study, a pooled clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) strategy targeting three GmLox genes (GmLox1, GmLox2, and GmLox3) was applied and 60 T-0 positive transgenic plants were generated, carrying combinations of sgRNAs and mutations. Among them, GmLox-28 and GmLox-60 were gmlox1gmlox2gmlox3 triple mutants and GmLox-40 was a gmlox1gmlox2 double mutant. Sequencing of T-1 mutant plants derived from GmLox-28, GmLox-60, and GmLox-40 showed that mutation in the GmLox gene was inherited by the next generation. Colorimetric assay revealed that plants carrying different combinations of mutations lost the corresponding lipoxygenase activities. Transgene-free mutants were obtained by screening the T-2 generation of lipoxygenase-free mutant lines (GmLox-28 and GmLox-60). These transgene- and lipoxygenase-free mutants could be used for soybean beany flavor reduction without restriction by regulatory frameworks governing transgenic organisms. (C) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAiCommunications Co., Ltd.
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| GB/T 7714 | Wang, Jie , Kuang, Huaqin , Zhang, Zhihui et al. Generation of seed lipoxygenase-free soybean using CRISPR-Cas9 [J]. | CROP JOURNAL , 2020 , 8 (3) : 432-439 . |
| MLA | Wang, Jie et al. "Generation of seed lipoxygenase-free soybean using CRISPR-Cas9" . | CROP JOURNAL 8 . 3 (2020) : 432-439 . |
| APA | Wang, Jie , Kuang, Huaqin , Zhang, Zhihui , Yang, Yongqing , Yan, Long , Zhang, Mengchen et al. Generation of seed lipoxygenase-free soybean using CRISPR-Cas9 . | CROP JOURNAL , 2020 , 8 (3) , 432-439 . |
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Abstract ：
Beany flavor induced by three lipoxygenases (LOXs, including LOX1, LOX2, and LOX3) restricts human consumption of soybean. It is desirable to generate lipoxygenase-free new mutant lines to improve the eating quality of soybean oil and protein products. In this study, a pooled clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) strategy targeting three GmLox genes (GmLox1, GmLox2, and GmLox3) was applied and 60 T0 positive transgenic plants were generated, carrying combinations of sgRNAs and mutations. Among them, GmLox-28 and GmLox-60 were gmlox1gmlox2gmlox3 triple mutants and GmLox-40 was a gmlox1gmlox2 double mutant. Sequencing of T1 mutant plants derived from GmLox-28, GmLox-60, and GmLox-40 showed that mutation in the GmLox gene was inherited by the next generation. Colorimetric assay revealed that plants carrying different combinations of mutations lost the corresponding lipoxygenase activities. Transgene-free mutants were obtained by screening the T2 generation of lipoxygenase-free mutant lines (GmLox-28 and GmLox-60). These transgene-and lipoxygenase-free mutants could be used for soybean beany flavor reduction without restriction by regulatory frameworks governing transgenic organisms.
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| GB/T 7714 | Jie Wang , Huaqin Kuang , Zhihui Zhang et al. Generation of seed lipoxygenase-free soybean using CRISPR-Cas9 [J]. | 作物学报（英文版） , 2020 , 8 (3) : 432-439 . |
| MLA | Jie Wang et al. "Generation of seed lipoxygenase-free soybean using CRISPR-Cas9" . | 作物学报（英文版） 8 . 3 (2020) : 432-439 . |
| APA | Jie Wang , Huaqin Kuang , Zhihui Zhang , Yongqing Yang , Long Yan , Mengchen Zhang et al. Generation of seed lipoxygenase-free soybean using CRISPR-Cas9 . | 作物学报（英文版） , 2020 , 8 (3) , 432-439 . |
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Soybean (Glycine max [L.] Merr.) is an important source of human dietary protein and vegetable oil. A strong negative correlation between protein and oil contents has hindered efforts to improve soybean seed quality. The metabolic and genetic bases of soybean seed composition remain elusive. We evaluated metabolic diversity in a soybean near-isogenic line (NIL) population derived from parents (JD12 and CMSD) with contrasting seed oil contents. Using CC-TOF/MS, we compared seed primary metabolites of high protein/low oil lines, low protein/high oil lines, and their parents. Principal-components analysis showed that metabolic profiles of all progeny lines could be discriminated based on protein and oil contents. Univariate analysis revealed wide variation and transgressive segregation of metabolites in the population. Twenty-eight annotated metabolites, in particular free asparagine, free 3-cyanoalanine, and L-malic acid, were correlated with seed protein content or seed oil content or seed protein and oil content. These results shed light on the metabolic and genetic basis of soybean seed composition. (C) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
Keyword ：
Metabolomics Metabolomics Near-isogenic population Near-isogenic population Oil Oil Protein Protein Seed composition Seed composition
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| GB/T 7714 | Wang, Jie , Zhou, Pengfei , Shi, Xiaolei et al. Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean [J]. | CROP JOURNAL , 2019 , 7 (5) : 651-659 . |
| MLA | Wang, Jie et al. "Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean" . | CROP JOURNAL 7 . 5 (2019) : 651-659 . |
| APA | Wang, Jie , Zhou, Pengfei , Shi, Xiaolei , Yang, Na , Yan, Long , Zhao, Qingsong et al. Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean . | CROP JOURNAL , 2019 , 7 (5) , 651-659 . |
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