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学者姓名：张仟
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Abstract ：
Heat stress significantly impacts global rice production, highlighting the critical need to understand the genetic basis of heat resistance in rice. U2AF (U2 snRNP auxiliary factor) is an essential splicing complex with critical roles in recognizing the 3 '-splice site of precursor messenger RNAs (pre-mRNAs). The U2AF small subunit (U2AF35) can bind to the 3 '-AG intron border and promote U2 snRNP binding to the branch-point sequences of introns through interaction with the U2AF large subunit (U2AF65). However, the functions of U2AF35 in plants are poorly understood. In this study, we discovered that the OsU2AF35a gene was vigorously induced by heat stress and could positively regulate rice thermotolerance during both the seedling and reproductive growth stages. OsU2AF35a interacts with OsU2AF65a within the nucleus, and both of them can form condensates through liquid-liquid phase separation (LLPS) following heat stress. The intrinsically disordered regions (IDR) are accountable for their LLPS. OsU2AF35a condensation is indispensable for thermotolerance. RNA-seq analysis disclosed that, subsequent to heat treatment, the expression levels of several genes associated with water deficiency and oxidative stress in osu2af35a-1 were markedly lower than those in ZH11. In accordance with this, OsU2AF35a is capable of positively regulating the oxidative stress resistance of rice. The pre-mRNAs of a considerable number of genes in the osu2af35a-1 mutant exhibited defective splicing, among which was the OsHSA32 gene. Knocking out OsHSA32 significantly reduced the thermotolerance of rice, while overexpressing OsHSA32 could partially rescue the heat sensitivity of osu2af35a-1. Together, our findings uncovered the essential role of OsU2AF35a in rice heat stress response through protein separation and regulating alternative pre-mRNA splicing.
Keyword ：
condensation condensation liquid-liquid phase separation liquid-liquid phase separation OsHSA32 OsHSA32 OsU2AF35a OsU2AF35a pre-mRNA splicing pre-mRNA splicing rice thermotolerance rice thermotolerance
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| GB/T 7714 | Liu, Jianping , Li, Xin , Wang, Ke et al. The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre-mRNA in rice [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (4) : 1308-1328 . |
| MLA | Liu, Jianping et al. "The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre-mRNA in rice" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 4 (2025) : 1308-1328 . |
| APA | Liu, Jianping , Li, Xin , Wang, Ke , Wang, Tao , Meng, Yang , Peng, Zhi et al. The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre-mRNA in rice . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (4) , 1308-1328 . |
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Understanding the regulatory mechanisms underlying the plant heat stress response is important for developing climate-resilient crops, including rice (Oryza sativa). Here, we report that OsEIL5, one member of the ETHYLENE INSENSITIVE3-LIKE family, positively regulates rice heat tolerance at the seedling and reproductive stages. OsEIL5 directly binds to the promoter of OsPP91 (encoding one type 2C protein phosphatase) and activates its expression. OsPP91 is required for rice thermotolerance, and overexpressing OsPP91 in oseil5-1 partially rescues its heat sensitivity. The F box protein OsEBF1 interacts with OsEIL5 and degrades it through the ubiquitination pathway, resulting in the reduction of OsPP91 expression and ultimately weakening rice heat tolerance. Knocking out OsEIL5 in the EBF1R13 line partially reduces its extremely high heat tolerance. Taken together, our work uncovers a mechanism that finely regulates rice thermotolerance through the OsEBF1-OsEIL5-OsPP91 module at the posttranslational and transcriptional levels.
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| GB/T 7714 | Liu, Jianping , Wang, Ke , Wang, Guining et al. The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation [J]. | CELL REPORTS , 2025 , 44 (2) . |
| MLA | Liu, Jianping et al. "The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation" . | CELL REPORTS 44 . 2 (2025) . |
| APA | Liu, Jianping , Wang, Ke , Wang, Guining , Peng, Zhi , Wang, Tao , Meng, Yang et al. The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation . | CELL REPORTS , 2025 , 44 (2) . |
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White lupin (Lupinus albus L.) produces cluster roots to acquire more phosphorus under phosphorus deficiency. Bacillus amyloliquefaciens SQR9 contributes to plant growth, but whether and how it promotes cluster root formation in white lupin remain unclear. Here, we investigated the roles of SQR9 in cluster root formation under low phosphorus conditions using a microbial mutant and virus-induced gene silencing (VIGS) in white lupin. SQR9 substantially enhanced cluster root formation under low phosphorus conditions. The ysnE gene encodes an auxin biosynthesis enzyme in SQR9 and was associated with cluster root formation, as ysnE-defective SQR9 did not trigger cluster root formation. SQR9 inoculation induced the expression of PIN-formed2 (LaPIN2, encoding an auxin transporter) and YUCCA4 (LaYUC4, encoding an auxin biosynthesis enzyme) in white lupin roots. VIGS-mediated knockdown of LaPIN2 and LaYUC4 prevented wild-type SQR9-induced cluster root formation in white lupin. Finally, white lupin LaYUC4-derived auxin and SQR9-derived auxin pools were both transported by LaPIN2, promoting cluster root formation under low phosphorus conditions. Taken together, we propose that B. amyloliquefaciens promotes cluster root formation in white lupin under low phosphorus conditions by stimulating auxin biosynthesis and transport. Our results provide insights into the interplay between bacteria and root auxin in crop phosphorus use efficiency. Bacillus amyloliquefaciens produces auxin and promotes auxin biosynthesis in white lupin under low phosphorus conditions, triggering cluster root formation.
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| GB/T 7714 | Yang, Jinyong , Li, Shenglan , Zhou, Xiangxue et al. Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels [J]. | PLANT PHYSIOLOGY , 2025 , 197 (2) . |
| MLA | Yang, Jinyong et al. "Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels" . | PLANT PHYSIOLOGY 197 . 2 (2025) . |
| APA | Yang, Jinyong , Li, Shenglan , Zhou, Xiangxue , Du, Chongxuan , Fang, Ju , Li, Xing et al. Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels . | PLANT PHYSIOLOGY , 2025 , 197 (2) . |
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White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.
Keyword ：
cluster root formation cluster root formation DNA methylation DNA methylation melatonin synthesis melatonin synthesis phosphorus deficiency phosphorus deficiency white lupin white lupin
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| GB/T 7714 | Zhang, Qian , Li, Xing , Geng-Li, Jiahong et al. DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) : 8548-8560 . |
| MLA | Zhang, Qian et al. "DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis" . | PLANT CELL AND ENVIRONMENT 48 . 12 (2025) : 8548-8560 . |
| APA | Zhang, Qian , Li, Xing , Geng-Li, Jiahong , Yang, Jinyong , Liu, Jingyi , Wang, Ke et al. DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) , 8548-8560 . |
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Rhizosheath formation is facilitated by root hair length, root exudates, the soil microbes, which collectively enhance plant resistance to drought. This process partly results from the complex interaction between root exudates and microbes, a relationship that remains poorly understood. The roles of root exudates and microbes in rhizosheath formation in rice under soil drying (SD) conditions are investigated. In tetraploid rice, rhizosheath formation under SD is approximately 70% greater than in diploid rice. Inoculation of diploid rice with the rhizosheath soil microbiota from tetraploid rice significantly enhanced rhizosheath formation under SD. The bacterial genus Pseudomonas is identified as the key taxon promoting rhizosheath formation in tetraploid rice under SD. Tetraploid rice exhibits significantly higher root flavonoid concentration than diploid rice under SD. Overexpression of the chalcone synthase gene (OsCHS1), a key gene involved in flavonoid biosynthesis, led to a significant increase in the abundance of Pseudomonadaceae in diploid rice. Pseudomonas nitroreducens, isolated from the rhizosheath of tetraploid rice, demonstrates chemotactic attraction to flavonoids, but this behavior is not observed in histidine kinase mutant Delta cheA. Diploid and tetraploid rice inoculated with P. nitroreducens and IAA biosynthesis complemented strain Delta iaaM-c formed larger rhizosheath under SD than those inoculated with its IAA biosynthesis mutant Delta iaaM. These results suggest that auxin-producing Pseudomonas, recruited by root flavonoids, enhances rice rhizosheath formation through the bacterial histidine kinase under SD. This finding may facilitate the improvement of environmental adaptation in polyploidy crops by regulating their interactions with beneficial soil microorganisms.
Keyword ：
polyploidy polyploidy pseudomonas pseudomonas rhizosheath formation rhizosheath formation rice rice soil drying soil drying
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| GB/T 7714 | Xu, Feiyun , Wang, Yongsen , Yang, Jinyong et al. Auxin-Producing Pseudomonas Recruited by Root Flavonoids Increases Rice Rhizosheath Formation through the Bacterial Histidine Kinase Under Soil Drying [J]. | ADVANCED SCIENCE , 2025 , 12 (34) . |
| MLA | Xu, Feiyun et al. "Auxin-Producing Pseudomonas Recruited by Root Flavonoids Increases Rice Rhizosheath Formation through the Bacterial Histidine Kinase Under Soil Drying" . | ADVANCED SCIENCE 12 . 34 (2025) . |
| APA | Xu, Feiyun , Wang, Yongsen , Yang, Jinyong , Zhang, Xue , Wang, Ke , Ding, Fan et al. Auxin-Producing Pseudomonas Recruited by Root Flavonoids Increases Rice Rhizosheath Formation through the Bacterial Histidine Kinase Under Soil Drying . | ADVANCED SCIENCE , 2025 , 12 (34) . |
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本发明公开了LaPUCHI1基因及其编码蛋白在调控缺磷条件下白羽扇豆排根形成中的应用。所述LaPUCHI1基因的核苷酸序列如SEQ ID NO.1所示，其编码的蛋白的氨基酸序列如SEQ ID NO.2所示。本发明研究发现，LaPUCHI1基因正调控缺磷条件下白羽扇豆排根形成，过表达白羽扇豆的LaPUCHI1基因促进缺磷条件下白羽扇豆排根形成，沉默或敲除白羽扇豆的LaPUCHI1基因抑制缺磷条件下白羽扇豆排根形成。LaPUCHI1基因为缺磷环境下白羽扇豆排根形成的关键调控基因，本发明为白羽扇豆的缺磷环境下排根形成提供了宝贵的基因资源。
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| GB/T 7714 | 许卫锋 , 杨金勇 , 周项雪 et al. LaPUCHI1基因及其编码蛋白在调控缺磷条件下白羽扇豆排根形成中的应用 : CN202411716087.2[P]. | 2024-11-27 . |
| MLA | 许卫锋 et al. "LaPUCHI1基因及其编码蛋白在调控缺磷条件下白羽扇豆排根形成中的应用" : CN202411716087.2. | 2024-11-27 . |
| APA | 许卫锋 , 杨金勇 , 周项雪 , 李幸 , 许飞云 , 张仟 et al. LaPUCHI1基因及其编码蛋白在调控缺磷条件下白羽扇豆排根形成中的应用 : CN202411716087.2. | 2024-11-27 . |
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Phosphorus is critical for plant growth but often becomes less accessible due to its precipitation with cations in soil. Fabaceae, a diverse plant family, exhibits robust adaptability and includes species like Lupinus albus, known for its efficient phosphorus utilization via cluster roots. Here, we systematically identified phosphorus-utilization-efficiency (PUE) gene families across 35 Fabaceae species, highlighting significant gene amplification in PUE pathways in Fabaceae. Different PUE pathways exhibited variable amplification, evolution, and retention patterns among various Fabaceae crops. Additionally, the number of homologous genes of the root hair development gene RSL2 in L. albus was far more than that in other Fabaceae species. Multiple copies of the RSL2 gene were amplified and retained in L. albus after whole genome triplication. The gene structure and motifs specifically retained in L. albus were different from homologous genes in other plants. Combining transcriptome analysis under low-phosphorus treatment, it was found that most of the homologous genes of RSL2 in L. albus showed high expression in the cluster roots, suggesting that the RSL2 gene family plays an important role in the adaptation process of L. albus to low-phosphorus environments and the formation of cluster roots.
Keyword ：
cluster root cluster root Fabaceae Fabaceae phosphorus utilization efficiency phosphorus utilization efficiency RSL2 RSL2 white lupin white lupin
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| GB/T 7714 | Li, Xing , Yang, Jinyong , Zhang, Qian et al. Phosphorus-Use-Efficiency Gene Identification in Fabaceae and RSL2 Expansion in Lupinus albus Is Associated with Low-Phosphorus Adaptation [J]. | GENES , 2024 , 15 (8) . |
| MLA | Li, Xing et al. "Phosphorus-Use-Efficiency Gene Identification in Fabaceae and RSL2 Expansion in Lupinus albus Is Associated with Low-Phosphorus Adaptation" . | GENES 15 . 8 (2024) . |
| APA | Li, Xing , Yang, Jinyong , Zhang, Qian , Zhang, Lingkui , Cheng, Feng , Xu, Weifeng . Phosphorus-Use-Efficiency Gene Identification in Fabaceae and RSL2 Expansion in Lupinus albus Is Associated with Low-Phosphorus Adaptation . | GENES , 2024 , 15 (8) . |
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本发明公开LaCOMT1蛋白及其编码基因在白羽扇豆响应低磷环境下排根形成中的应用。本发明研究了白羽扇豆在低磷环境下排根形成的通路，发现褪黑素通路参与白羽扇豆在低磷环境下的排根形成，且鉴定了褪黑素通路中的LaCOMT1基因为低磷环境下白羽扇豆排根形成的关键调控基因。本发明人经进一步研究发现，褪黑素通路中关键调控基因LaCOMT1的甲基化升高，会引起基因表达的升高并促进低磷环境下根部褪黑素含量的积累，从而促进排根形成。本发明为白羽扇豆的低磷环境下排根形成提供了宝贵的基因资源。
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| GB/T 7714 | 李幸 , 许卫锋 , 张仟 et al. LaCOMT1蛋白及其编码基因在白羽扇豆响应低磷环境下排根形成中的应用 : CN202410673942.X[P]. | 2024-05-28 . |
| MLA | 李幸 et al. "LaCOMT1蛋白及其编码基因在白羽扇豆响应低磷环境下排根形成中的应用" : CN202410673942.X. | 2024-05-28 . |
| APA | 李幸 , 许卫锋 , 张仟 , 杨金勇 . LaCOMT1蛋白及其编码基因在白羽扇豆响应低磷环境下排根形成中的应用 : CN202410673942.X. | 2024-05-28 . |
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本发明公开了一株硝基还原假单胞菌及其在水稻抗旱、促生中的应用，所述菌株分类命名为Pseudomonas nitroreducens LSF‑6，已于2022年5月12日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 2022607。本发明提供的硝基还原假单胞菌LSF‑6能够在干旱条件下通过调控水稻根鞘建成，促进水稻的生长及并提高其水分利用率，进而增强水稻的耐干旱胁迫能力，具有较好的应用前景。
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| GB/T 7714 | 许飞云 , 许卫锋 , 袁伟 et al. 一株硝基还原假单胞菌及其在水稻抗旱、促生中的应用 : CN202211096063.2[P]. | 2022-09-06 . |
| MLA | 许飞云 et al. "一株硝基还原假单胞菌及其在水稻抗旱、促生中的应用" : CN202211096063.2. | 2022-09-06 . |
| APA | 许飞云 , 许卫锋 , 袁伟 , 张仟 , 刘建平 , 王珂 . 一株硝基还原假单胞菌及其在水稻抗旱、促生中的应用 : CN202211096063.2. | 2022-09-06 . |
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本发明公开了一株白羽扇豆内生细菌CRB1及其应用。所述白羽扇豆内生细菌CRB1的分类命名为Klebsiella sp.CRB1，其16S rDNA基因序列如SEQ ID NO.1所述，该菌株已于2022年5月12日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 2022606。本发明首次从白羽扇豆的排根中分离筛选到内生细菌CRB1，该菌可在低磷环境下促进白羽扇豆的生长，具体表现为促进白羽扇豆排根的生长，有助于白羽扇豆适应低磷环境，为白羽扇豆的种植领域带来广阔的应用前景。
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| GB/T 7714 | 张仟 , 许卫锋 , 丁业新 et al. 一株白羽扇豆内生细菌CRB1及其应用 : CN202211080010.1[P]. | 2022-09-05 . |
| MLA | 张仟 et al. "一株白羽扇豆内生细菌CRB1及其应用" : CN202211080010.1. | 2022-09-05 . |
| APA | 张仟 , 许卫锋 , 丁业新 , 许飞云 , 杨金勇 , 王月 et al. 一株白羽扇豆内生细菌CRB1及其应用 : CN202211080010.1. | 2022-09-05 . |
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