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学者姓名：吴良泉
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Abstract ：
Quantifying nitrogen (N) application thresholds is crucial for harmonizing productivity with water quality protection in intensive agricultural systems. Using Pinghe County, China's the largest pomelo production region, as a case study, this study establishes scientifically grounded N thresholds to counter the threats pose by excessive fertilizer inputs to sustainable production and water safety. Based on 954 farmer surveys and three years (2021-2023) of river monitoring, we fitted linear-plateau models relating N application to yield for honey pomelo (Citrus grandis L. Osbeck) and grapefruit (Citrus x paradisi), and a two-segment linear model linking watershed N balance with river NO3--N concentrations. Current N application rates averaged 359.9 kg N ha-1 yr-1 for honey pomelo and 374.0 kg N ha-1 yr-1 for grapefruit, with low N use efficiencies (NUE) of 32.4 % and 30.7 %, respectively. Piecewise regression identified optimal yield-based N application thresholds of 186.5-230.7 kg N ha-1 for honey pomelo and 187.0-222.8 kg N ha-1 for grapefruit. River NO3--N concentrations increased sharply when watershed N balance exceeded 117.7 kg N ha-1, and maintaining NO3--N below the 10 mg L-1 safety limit requires restricting N application rates not exceed 192.2 kg N ha-1. Scenario analysis indicated that N input based on yield optimization and water quality safety could achieve honey pomelo and grapefruit NUE of 39.6-41.7 % and 41.7-42.5 %, respectively. Overall, this study defines a safe N boundary that balances productivity with river water safety and provides actionable guidance for N management in intensive pomelo systems.
Keyword ：
Nitrate nitrogen Nitrate nitrogen Nitrogen balance Nitrogen balance Nitrogen threshold Nitrogen threshold Pomelo yield Pomelo yield
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| GB/T 7714 | Luo, Ziwei , Li, Ran , Hu, Xiaotong et al. Safe nitrogen boundaries for sustainable pomelo production: A case study in the hilly regions of southern China [J]. | AGRICULTURE ECOSYSTEMS & ENVIRONMENT , 2026 , 398 . |
| MLA | Luo, Ziwei et al. "Safe nitrogen boundaries for sustainable pomelo production: A case study in the hilly regions of southern China" . | AGRICULTURE ECOSYSTEMS & ENVIRONMENT 398 (2026) . |
| APA | Luo, Ziwei , Li, Ran , Hu, Xiaotong , Liu, Yedong , He, Zhiyuan , Yang, Ruixiang et al. Safe nitrogen boundaries for sustainable pomelo production: A case study in the hilly regions of southern China . | AGRICULTURE ECOSYSTEMS & ENVIRONMENT , 2026 , 398 . |
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本发明公开了一种促进茶园土壤固碳减排的镁肥施用装置，涉及施肥领域，包括小推车、设置于小推车顶部的储料桶、固定连接在储料桶底部的排料机构，以及固定连接在储料桶顶部的混合箱，还包括与储料桶相连接的打散机构、与混合箱相连接的搅拌机构，以及设置于储料桶与混合箱内部的分隔机构；打散机构包括贯穿储料桶侧壁并与侧壁转动连接的驱动轴，以及固定连接在驱动轴外侧壁用于带动所述驱动轴旋转的传动杆；一种促进茶园土壤固碳减排的镁肥施用装置的施用方法，包括配料、混合、下料与再混合以及施肥。本发明为一种促进茶园土壤固碳减排的镁肥施用装置及施用方法，通过将振动力转为驱动轴的驱动力，来提高行驶的稳定性。
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| GB/T 7714 | 郭梨锦 , 林蔚 , 廖鹏辉 et al. 一种促进茶园土壤固碳减排的镁肥施用装置及施用方法 : CN202411844517.9[P]. | 2024-12-16 . |
| MLA | 郭梨锦 et al. "一种促进茶园土壤固碳减排的镁肥施用装置及施用方法" : CN202411844517.9. | 2024-12-16 . |
| APA | 郭梨锦 , 林蔚 , 廖鹏辉 , 吴良泉 , 王培杰 , 何正嘉 et al. 一种促进茶园土壤固碳减排的镁肥施用装置及施用方法 : CN202411844517.9. | 2024-12-16 . |
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本发明公开了一种有机肥定量化施肥控制装置，涉及农业种植设备技术领域。本发明包括主体，主体上方设置有连接件，连接件上方设置有搅拌桶，搅拌桶内部设置有搅拌机构，搅拌机构用于对搅拌桶内部的有机肥进行均匀搅拌，搅拌桶两侧设置有波纹管，波纹管与连接管相互连通，连接管连通有定量机构，定量机构用于对有机肥进行定量均匀播撒。本发明通过搅拌机构对有机肥料进行均匀的搅拌，防止混料不均，再利用双头驱动气缸带动两侧的打孔机构向地面打下施肥孔，随后启动定量机构均匀的排出有机肥料，还可以根据需要调节有机肥料的流速，最后在启动覆土机构，从而对施肥孔两侧的土壤进行覆土作业。
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| GB/T 7714 | 吴良泉 . 一种有机肥定量化施肥控制装置 : CN202510700803.6[P]. | 2025-05-28 . |
| MLA | 吴良泉 . "一种有机肥定量化施肥控制装置" : CN202510700803.6. | 2025-05-28 . |
| APA | 吴良泉 . 一种有机肥定量化施肥控制装置 : CN202510700803.6. | 2025-05-28 . |
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Nitrogen (N) is essential for maintaining crop productivity, but its excessive input in agriculture leads to significant environmental challenges. This study investigates the N input in pomelo production in Pinghe County, which is one of the largest pomelo production regions in China, where overuse of N fertilizers in orchards has raised concerns about water quality and sustainable production. Based on 954 farmer survey questionnaires and three consecutive years (2021-2023) of river water quality monitoring, this study established the relationships between N input and yield for two major cultivated pomelo varieties—honey pomelo (Citrus grandis L. Osbeck) and grapefruit (Citrus × paradisi)—as well as between sub-watersheds N balance and river NO3--N concentrations. The results reveal current N application rate averaging 359.9 kg N ha-1 yr-1 for honey pomelo and 374.0 kg N ha-1 yr-1 for grapefruit, with N use efficiency (NUE) of 32.4% and 30.7%, respectively. When N application rate exceeded a certain threshold, yield growth stagnated. Using piecewise regression, we determined the recommended N application thresholds based on yield responses at different elevations for honey pomelo and grapefruit to be 186.5–230.7 and 187.0–222.8 kg ha-1, respectively. NO3--N concentrations increased sharply when the watershed N balance exceeded 117.7 kg ha-1. To keep river NO3--N concentrations below the river water safety limit of 10 mg L-1, we recommend that N fertilizer application rate should not exceed 192.2 kg N ha-1. Scenario analysis found that N input based on yield optimization and water quality safety could achieve NUE of 39.6–41.7% and 41.7–42.5%, respectively. Overall, the study established safety N boundary for citrus production based on yield and river water quality safety. This study provides empirical evidence and actionable guidelines for developing sustainable, ecologically integrated nitrogen management strategies in intensive agricultural systems. © 2025, The Authors. All rights reserved.
Keyword ：
Citrus fruits Citrus fruits Nitrogen Nitrogen Nitrogen fertilizers Nitrogen fertilizers Rivers Rivers Sustainable development Sustainable development Water quality Water quality Watersheds Watersheds
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| GB/T 7714 | Luo, Ziwei , Li, Ran , Hu, Xiaotong et al. Safe nitrogen boundaries for sustainable pomelo production: A case study in the hilly regions of southern China [J]. | SSRN , 2025 . |
| MLA | Luo, Ziwei et al. "Safe nitrogen boundaries for sustainable pomelo production: A case study in the hilly regions of southern China" . | SSRN (2025) . |
| APA | Luo, Ziwei , Li, Ran , Hu, Xiaotong , Liu, Yedong , He, Zhiyuan , Yang, Ruixiang et al. Safe nitrogen boundaries for sustainable pomelo production: A case study in the hilly regions of southern China . | SSRN , 2025 . |
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. Pomelo quality grading helps to control the quality of pomelo production and promotes a standardized production. The realization of automated pomelo quality grading relies on the acquisition of several phenotypic parameters, with pomelo volume being a crucial one. Fruit phenotypic features, such as projected area, perimeter, radial distance, and the maximum radius of the tangent circle, are often used to estimate fruit volume through image processing. To enhance the accuracy of volume estimation, this article introduces a pomelo volume estimation model that relies on image features. This model is based on an analysis of the fruits' symmetry axis and combines various features, including the projected area, perimeter, radial distance, and the maximum radius of the tangent circle. In this study, we collected a dataset of 3,200 pomelo images and compared the prediction results under different parameter combinations. After incorporating the parameters associated with the symmetry axis, the root mean square error of volume prediction was 159.82. The coefficient of determination was 0.84, with a mean absolute error of 142.67 and a mean absolute percentage error of 0.23. Notably, the coefficient of determination was improved by 0.26 compared to the results obtained without considering the symmetry axis features. The prediction error falls within the acceptable range, meeting the requirements of practical application. Overall, the proposed method provides a valuable technical reference for pomelo quality grading.
Keyword ：
Axis of symmetry Axis of symmetry Feature parameters Feature parameters Machine learning Machine learning Volume prediction Volume prediction
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| GB/T 7714 | Lin, Yaohai , Yang, Enqi , Chen, Wei et al. VOLUME PREDICTION METHOD FOR POMELO BASED ON SYMMETRY AXIAL [J]. | APPLIED ENGINEERING IN AGRICULTURE , 2025 , 41 (2) : 195-204 . |
| MLA | Lin, Yaohai et al. "VOLUME PREDICTION METHOD FOR POMELO BASED ON SYMMETRY AXIAL" . | APPLIED ENGINEERING IN AGRICULTURE 41 . 2 (2025) : 195-204 . |
| APA | Lin, Yaohai , Yang, Enqi , Chen, Wei , Lin, Chuancong , Chen, Riqing , Yang, Changcai et al. VOLUME PREDICTION METHOD FOR POMELO BASED ON SYMMETRY AXIAL . | APPLIED ENGINEERING IN AGRICULTURE , 2025 , 41 (2) , 195-204 . |
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To explore the effects of reducing nitrogen (N) fertilizer application and natural mulch on yield, quality, soil physicochemical properties, and microbial community of pomelo (Citrus grandis), thereby providing a theoretical basis for techniques to reduce chemical fertilizer use while improving quality and efficiency in pomelo cultivation. A two-year field experiment was conducted with three treatments: (1) farmer's N fertilizer (F) application practice (1084 kg N ha- 1 yr- 1), (2) farmer's N fertilizer + natural grass cover crops (FN) (1084 kg N ha- 1 yr- 1), and (3) optimized N application + natural grass cover crops (ON) (758.8 kg N ha- 1 yr- 1, a 30% reduction in N compared to FN). The results showed that compared with F, the FN and ON increased vitamin C content, flesh aspect ratio, and the ratio of total soluble solids to titratable acidity while decreasing the titratable acidity. In addition, the FN and ON improved soil water content, organic matter, and pH while decreasing exchangeable acidity and bulk density. Metagenomic sequencing results revealed that FN increased the diversity of bacterial and fungal communities. Functional analysis indicated that ON significantly increased the abundance of functional genes involved in carbon metabolism. Furthermore, FN and ON significantly affected the abundance of the carbohydrate-active enzymes (CAZy) gene at the class level. Correlation analysis demonstrated that soil properties, microbial communities, and functional attributes all influenced the pomelo quality. In summary, reducing N fertilizer application and incorporating natural grass cover crops can serve as an effective management strategy to improve pomelo yield and quality.
Keyword ：
Functional genes Functional genes Grass cover crops Grass cover crops Pomelo productivity and quality Pomelo productivity and quality Reduction N fertilizer Reduction N fertilizer Soil acidification Soil acidification Soil microorganisms Soil microorganisms
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| GB/T 7714 | Zou, Shuang , Ji, Zongjun , Rao, Xinhao et al. Response of Fruit Productivity, Soil Physicochemical Properties, and Microbiota to Nitrogen Reduction and Grass Cover Crops in Pomelo Orchard [J]. | JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION , 2025 , 25 (2) : 4517-4530 . |
| MLA | Zou, Shuang et al. "Response of Fruit Productivity, Soil Physicochemical Properties, and Microbiota to Nitrogen Reduction and Grass Cover Crops in Pomelo Orchard" . | JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION 25 . 2 (2025) : 4517-4530 . |
| APA | Zou, Shuang , Ji, Zongjun , Rao, Xinhao , Guo, Yingmin , Muneer, Muhammad Atif , Zhang, Siwen et al. Response of Fruit Productivity, Soil Physicochemical Properties, and Microbiota to Nitrogen Reduction and Grass Cover Crops in Pomelo Orchard . | JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION , 2025 , 25 (2) , 4517-4530 . |
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Excessive nitrogen fertilizer application in tea plantations is a common problem that leads to soil acidification and reductions in both yield and economic returns. To evaluate the impacts of varying nitrogen input levels (0-600 kg N ha-1 yr-1) on yield, as well as environmental and economic outcomes, a two-year field experiment was conducted. Results demonstrated that when nitrogen application exceeded 150 kg N ha-1 yr-1, key efficiency indicators-agronomic efficiency (NAE), recovery efficiency (NRE), and partial factor productivity (NPFP) declined markedly. Among all treatments, the 150 kg N ha-1 yr-1 rate achieved the highest NRE at 28.01%. Moreover, environmental burdens including global warming potential, acidification, and eutrophication intensified with increasing nitrogen input. Benefit analysis revealed that yield-based economic returns peaked between 150 and 165 kg N ha-1 yr-1, whereas the highest ecosystem economic benefit, accounting for both profit and environmental cost, occurred at 120 kg N ha-1 yr-1. Thus, 120 kg N ha-1 yr-1 is identified as the optimal application rate for maximizing integrated economic and environmental returns while maintaining yield. These findings provide valuable guidance for promoting sustainable nitrogen management in tea cultivation worldwide.
Keyword ：
economic benefits economic benefits nitrogen fertilizer application nitrogen fertilizer application sustainable tea production sustainable tea production tea tea
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| GB/T 7714 | Pei, Jinze , Yang, Hongyu , Huang, Menghan et al. Multi-Objective Nitrogen Optimization in Tea Cultivation: A Pathway to Achieve Sustainability in Cash Crop Systems [J]. | AGRICULTURE-BASEL , 2025 , 15 (18) . |
| MLA | Pei, Jinze et al. "Multi-Objective Nitrogen Optimization in Tea Cultivation: A Pathway to Achieve Sustainability in Cash Crop Systems" . | AGRICULTURE-BASEL 15 . 18 (2025) . |
| APA | Pei, Jinze , Yang, Hongyu , Huang, Menghan , Yan, Xiaojun , Zeng, Xinran , Guo, Lijin et al. Multi-Objective Nitrogen Optimization in Tea Cultivation: A Pathway to Achieve Sustainability in Cash Crop Systems . | AGRICULTURE-BASEL , 2025 , 15 (18) . |
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Magnesium (Mg) is essential for plant photosynthesis and redox balance. This study investigated the "photosynthetic inhibition-oxidative stress" cascade mechanism induced by Mg deficiency during the vegetative growth period (100 days after transplantation) of tobacco. A Mg gradient system (0, 0.25, 1.99 mM; Mg0, Mg1/8, Mg1) combined with multi-omics analysis revealed three mechanisms by which Mg0 impaired photosynthetic efficiency: (1) structural damage to the photosystem, including downregulation of light-harvesting complex (LHC) II /LHCI genes (NtLhcb2s and NtLhca3/4/6) and PSII core proteins (NtPsbD and NtPsbO1s); (2) disruption of chlorophyll metabolism, characterized by a significant decrease in NtCHs family gene expression, leading to abnormal chloroplast development; and (3) impaired electron transport (ET), confirmed by decreased quantum yield for ET (ET0/ABS) and maximum quantum yield of primary photochemistry (Fv/Fm), and damaged to the oxygen-evolving complex (OEC). Antioxidant genes (NtSOD, NtAPX, and NtGST) increase without a corresponding increase in enzyme activity, indicating transcript-enzyme decoupling; any reactive oxygen species (ROS)-dependent post-translational regulation remains to be tested. Mg1/8-treatment almost restored physiological balance by enhancing linear-ET efficiency and activating a Mg-independent ROS scavenging pathway (NtGSTs upregulated), although some stress markers remained above Mg1. These findings provide an integrated, system-level view of the impacts of Mg deficiency in tobacco and lay the groundwork for hypothesis-driven mechanistic studies.
Keyword ：
Mg-deficiency Mg-deficiency OJIP transients OJIP transients Oxidative stress Oxidative stress Photosynthesis Photosynthesis Transcriptome analysis Transcriptome analysis
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| GB/T 7714 | Xie, Rong-Rong , Gao, Jingjuan , Wu, Changzheng et al. Magnesium deficiency limits electron transport efficiency and photosynthesis, and induces oxidative stress in tobacco [J]. | PLANT SCIENCE , 2025 , 362 . |
| MLA | Xie, Rong-Rong et al. "Magnesium deficiency limits electron transport efficiency and photosynthesis, and induces oxidative stress in tobacco" . | PLANT SCIENCE 362 (2025) . |
| APA | Xie, Rong-Rong , Gao, Jingjuan , Wu, Changzheng , Guo, Jinping , Cheng, Qian , Wang, Hailu et al. Magnesium deficiency limits electron transport efficiency and photosynthesis, and induces oxidative stress in tobacco . | PLANT SCIENCE , 2025 , 362 . |
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Sweet corn, as a distinct maize type, is highly sensitive to nitrogen (N) management, yet the physiological mechanisms linking N supply with photosynthetic carbon assimilation and carbohydrate metabolism remain insufficiently understood. To address this, a two-year field experiment (2022-2023) was conducted using two cultivars (MT6855 and TYH6) under four N rates (0, 90, 180, and 270 kg N ha-1). Compared with no N, fresh ear yield increased by 2.5-, 4.6-, and 4.6-fold under 90, 180, and 270 kg N ha-1, respectively, with 180 kg N ha-1 identified as the optimal rate. At this rate, leaf SPAD, leaf area, and net photosynthetic rate increased significantly, accompanied by accelerated grain filling and enhanced accumulation of sucrose, fructose, and starch. Excessive N (270 kg N ha-1) reduced the activities of key carbohydrate-metabolizing enzymes by 7.8-12.4 % relative to 180 kg N ha-1, indicating impaired sink metabolism. Yield was strongly correlated with photosynthetic traits and enzyme activities, with vacuolar acid invertase identified as the major contributor. These findings demonstrate that optimal N fertilization enhances yield and quality through coordinated regulation of photosynthesis and carbohydrate metabolism, providing a mechanistic basis for developing efficient N management strategies in intensive sweet corn production.
Keyword ：
Carbohydrate allocation Carbohydrate allocation Carbon metabolism Carbon metabolism Enzyme activity Enzyme activity Grain-filling Grain-filling Starch synthase Starch synthase Sucrose Sucrose Sweet maize Sweet maize
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| GB/T 7714 | Ye, Delian , Yu, Zexun , Sun, Yanfang et al. Nitrogen-mediated coordination of photosynthesis and carbohydrate metabolism enhances sweet corn yield and quality [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 229 . |
| MLA | Ye, Delian et al. "Nitrogen-mediated coordination of photosynthesis and carbohydrate metabolism enhances sweet corn yield and quality" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 229 (2025) . |
| APA | Ye, Delian , Yu, Zexun , Sun, Yanfang , Gao, Wei , Zhang, Ang , Nisa, Zaib-Un et al. Nitrogen-mediated coordination of photosynthesis and carbohydrate metabolism enhances sweet corn yield and quality . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 229 . |
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为明确改良柚园酸化土壤最佳施肥方式，本研究通过为期两年的蜜柚田间试验，探究农户常规施肥（FFP）、优化施肥（OPT）和施用有机肥（OF）对表层（0～20 cm）和亚表层（20～40 cm）土壤pH值、养分含量、酶活性和土壤微生物群落的影响。结果表明，与FFP相比，OF处理可显著提高土壤pH值和有机质含量，而OPT处理使表层土壤有机质含量显著降低。与FFP相比，OF处理表层土壤蔗糖酶、脲酶、磷酸酶和过氧化氢酶活性显著增加，亚表层土壤脲酶和磷酸酶活性显著增加。与FFP相比，OPT和OF处理增加了土壤细菌多样性，而OF处理降低了真菌多样性。与FFP相比，OPT和OF处理使土壤优势细菌门中变形菌门相对丰度降低，而酸杆菌门相对丰度增加，且对亚表层土壤影响更明显。在优势真菌门中，与FFP相比，OPT处理担子菌门相对丰度降低，而子囊菌门相对丰度有所增加，OF处理则与其相反，且在表层土中效果更为明显。在优势细菌属中，与FFP相比，OPT和OF处理表层土壤慢生根瘤菌相对丰度有所增加，热酸菌相对丰度有所降低。在优势真菌属中，与FFP相比，OPT处理降低了表层土壤中原隐球菌和木霉菌以及亚表层土壤中原隐球菌的相对丰度；OF处理提升了表层土壤中原隐球菌以及亚表层土壤中原隐球菌和木霉菌的相对丰度。综上，优化施肥和施用有机肥可提升土壤pH值，增加微生物多样性，改善土壤微生物群落结构，激发土壤酶活性，为蜜柚树体的生长提供良好的土壤微生态环境，以施用有机肥效果更佳。本研究结果为蜜柚化肥减量与提质增效提供了技术参考。
Keyword ：
土壤pH值 土壤pH值 土壤酶活性 土壤酶活性 微生物群落 微生物群落 施肥 施肥 蜜柚 蜜柚
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| GB/T 7714 | 邹爽 , 武奥琳 , 潘住财 et al. 不同施肥处理对柚园土壤化学及微生物特性的影响 [J]. | 核农学报 , 2025 , 39 (04) : 827-838 . |
| MLA | 邹爽 et al. "不同施肥处理对柚园土壤化学及微生物特性的影响" . | 核农学报 39 . 04 (2025) : 827-838 . |
| APA | 邹爽 , 武奥琳 , 潘住财 , 饶新豪 , 吴良泉 , 冯人伟 et al. 不同施肥处理对柚园土壤化学及微生物特性的影响 . | 核农学报 , 2025 , 39 (04) , 827-838 . |
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