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学者姓名：郭梨锦
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Abstract ：
Bougainvillea is valued for its ornamental and medicinal properties, but its current propagation primarily relies on cuttings, which suffer from low efficiency. Although tissue culture offers faster propagation and is not limited by seasonal properties, the technical system remains underdeveloped. This study applies the Response Surface Methodology (RSM) to optimize the axillary bud induction and rooting stages of Bougainvillea tissue culture. By fitting a multivariate quadratic regression model to determine the relationship between factors and response values, the study aimed to refine the tissue culture system for Bougainvillea. The main findings of this study are as follows: (1) In the axillary bud induction stage, the optimal induction medium was MS (Murashige and Skoog) + 6-BA (6-Benzylaminopurine) 2.534 mg L- 1 + NAA (1-Naphthaleneacetic acid) 0.1 mg L-1, achieving a bud induction rate of 93.3 %. (2) The ideal rooting medium consists of 0.66 MS + IBA (Indole-3-butyric acid) 2.0 mg L- 1 + NAA 0.09 mg L-1. Upon optimizing the culture system, the bud induction rate during the primary culture stage rose to 93.3 %, and the rooting rate during the rooting stage reached 66.7 %. In conclusion, the optimization of culture media using a multifactorial approach proved more effective than the traditional twofactor method, and more accurately optimizes the media of different stages, which provides valuable insights for improving the Bougainvillea tissue culture system.
Keyword ：
Bougainvillea Bougainvillea Response surface analysis Response surface analysis Tissue culture Tissue culture
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| GB/T 7714 | Li, Yu , Lin, Wei , Huang, Jing et al. Optimization of Bougainvillea tissue culture system by response surface methodology [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 226 . |
| MLA | Li, Yu et al. "Optimization of Bougainvillea tissue culture system by response surface methodology" . | INDUSTRIAL CROPS AND PRODUCTS 226 (2025) . |
| APA | Li, Yu , Lin, Wei , Huang, Jing , Ding, Jintao , Li, Jing , He, Bizhu et al. Optimization of Bougainvillea tissue culture system by response surface methodology . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 226 . |
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本发明涉及三角梅嫁接装置技术领域，公开了一种辅助三角梅高位嫁接造型的装置及其方法，包括：基座；导向机构，基座的内部设有导向机构；花盆，基座的内部通过导向机构设有花盆；嫁接机构，花盆的内部滑动连接有嫁接杆，嫁接杆的外侧壁拆卸连接有引导杆，引导杆的外侧壁设有嫁接机构，所述嫁接机构包含有嫁接环、第一齿轮环、第一锥齿轮、螺纹杆和嫁接块，所述引导杆的外侧壁设有嫁接环；通过导向机构和锁定机构可以根据不同角度的枝干进行角度调节，从而控制后期的生长方向，为后期的造型进行初步的把控，且通过嫁接机构可以快速的调节需要的高度然后对枝干和三角梅进行固定，便于后期的嫁接牢固，提高嫁接成功率。
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| GB/T 7714 | 郭梨锦 , 薛昕宇 , 林蔚 et al. 一种辅助三角梅高位嫁接造型的装置及其方法 : CN202510999349.9[P]. | 2025-07-21 . |
| MLA | 郭梨锦 et al. "一种辅助三角梅高位嫁接造型的装置及其方法" : CN202510999349.9. | 2025-07-21 . |
| APA | 郭梨锦 , 薛昕宇 , 林蔚 , 何碧珠 , 王宗博 , 杨宏宇 . 一种辅助三角梅高位嫁接造型的装置及其方法 : CN202510999349.9. | 2025-07-21 . |
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本发明涉及触探探头领域，公开了一种集成温室气体原位测量的静力触探探头与方法，包括设有气体检测器、温度检测器以及孔隙水压力探测头的检测探头，在检测探头插入土壤之前，固定杆处于折叠状态，闭合盖覆盖在空气窗口表面，起到保护作用，防止在插入土壤过程中土壤颗粒进入上腔室，对气体检测器和温度检测器造成损坏，同时，这种折叠状态使得检测探头的整体尺寸较小，便于插入土壤，并且当检测探头插入到预定深度后，电动推杆开始工作由于电动推杆呈倾斜状设置，其输出轴通过连接件与固定杆相连，当电动推杆伸长时，会推动固定杆绕转轴向外摆动，固定杆下部靠近电动推杆的一侧的平直状表面为受力点，使得固定杆能够平稳地向外展开。
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| GB/T 7714 | 郭梨锦 , 薛昕宇 , 沈昊鋮 et al. 一种集成温室气体原位测量的静力触探探头与方法 : CN202510998102.5[P]. | 2025-07-21 . |
| MLA | 郭梨锦 et al. "一种集成温室气体原位测量的静力触探探头与方法" : CN202510998102.5. | 2025-07-21 . |
| APA | 郭梨锦 , 薛昕宇 , 沈昊鋮 , 林蔚 , 王琳影 . 一种集成温室气体原位测量的静力触探探头与方法 : CN202510998102.5. | 2025-07-21 . |
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Excessive nitrogen fertilizer application in tea plantations is a common problem that leads to soil acidification and reductions in both yield and economic returns. To evaluate the impacts of varying nitrogen input levels (0-600 kg N ha-1 yr-1) on yield, as well as environmental and economic outcomes, a two-year field experiment was conducted. Results demonstrated that when nitrogen application exceeded 150 kg N ha-1 yr-1, key efficiency indicators-agronomic efficiency (NAE), recovery efficiency (NRE), and partial factor productivity (NPFP) declined markedly. Among all treatments, the 150 kg N ha-1 yr-1 rate achieved the highest NRE at 28.01%. Moreover, environmental burdens including global warming potential, acidification, and eutrophication intensified with increasing nitrogen input. Benefit analysis revealed that yield-based economic returns peaked between 150 and 165 kg N ha-1 yr-1, whereas the highest ecosystem economic benefit, accounting for both profit and environmental cost, occurred at 120 kg N ha-1 yr-1. Thus, 120 kg N ha-1 yr-1 is identified as the optimal application rate for maximizing integrated economic and environmental returns while maintaining yield. These findings provide valuable guidance for promoting sustainable nitrogen management in tea cultivation worldwide.
Keyword ：
economic benefits economic benefits nitrogen fertilizer application nitrogen fertilizer application sustainable tea production sustainable tea production tea tea
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| GB/T 7714 | Pei, Jinze , Yang, Hongyu , Huang, Menghan et al. Multi-Objective Nitrogen Optimization in Tea Cultivation: A Pathway to Achieve Sustainability in Cash Crop Systems [J]. | AGRICULTURE-BASEL , 2025 , 15 (18) . |
| MLA | Pei, Jinze et al. "Multi-Objective Nitrogen Optimization in Tea Cultivation: A Pathway to Achieve Sustainability in Cash Crop Systems" . | AGRICULTURE-BASEL 15 . 18 (2025) . |
| APA | Pei, Jinze , Yang, Hongyu , Huang, Menghan , Yan, Xiaojun , Zeng, Xinran , Guo, Lijin et al. Multi-Objective Nitrogen Optimization in Tea Cultivation: A Pathway to Achieve Sustainability in Cash Crop Systems . | AGRICULTURE-BASEL , 2025 , 15 (18) . |
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Cutting propagation is a crucial technique for woody ornamental plant reproduction. While the plant growth regulator naphthaleneacetic acid (NAA) and the beneficial fungus Piriformospora indica (Pi) are vital for adventitious root formation and cutting survival, the underlying mechanisms of their synergistic effects remain unclear. This study investigated the impact of NAA and Pi on rooting in cuttings of three distinct woody ornamentals: Rhododendron simsii, Jasminum sambac, and Rosa chinensis. Three treatments were applied: control group (CK, direct cutting), Pi inoculation (Pi), and NAA soaking followed by Pi inoculation (NAA+Pi). The results demonstrated that compared to CK, NAA+Pi significantly increased rooting rate (1.9 %-85.0 %) and survival rate (10.5 %-36.6 %), improved chlorophyll and free amino acid content, and enhanced plant height and fresh weight. Notably, NAA+Pi dramatically upregulated the expression of the key root development gene WOX4 by 2.6-4.0 fold, indicating a synergistic activation of WOX4 gene expression. The increased free amino acid content in the NAA+Pi treatment suggests enhanced secondary metabolism, providing essential resources for WOX expression and root development. All species demonstrated notable improvement, despite the fact that R. chinensis saw the most noticeable impacts. This underscores the considerable potential of using both NAA and Pi together to maximize the proliferation of woody ornamental cuttings. This research offers new insights for improving cutting propagation techniques.
Keyword ：
Naphthalene acetic acid Naphthalene acetic acid Piriformospora indica Piriformospora indica Rooting survival Rooting survival Woody ornamentals Woody ornamentals WOX gene WOX gene
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| GB/T 7714 | Tang, Xuexiao , Wang, Zongbo , Zong, Fanqi et al. Impact of naphthalene acetic acid and Piriformospora indica on WOX gene expression and rooting in woody ornamentals [J]. | SCIENTIA HORTICULTURAE , 2025 , 350 . |
| MLA | Tang, Xuexiao et al. "Impact of naphthalene acetic acid and Piriformospora indica on WOX gene expression and rooting in woody ornamentals" . | SCIENTIA HORTICULTURAE 350 (2025) . |
| APA | Tang, Xuexiao , Wang, Zongbo , Zong, Fanqi , Xue, Xinyu , Gao, Jiayuan , Li, Shanghuan et al. Impact of naphthalene acetic acid and Piriformospora indica on WOX gene expression and rooting in woody ornamentals . | SCIENTIA HORTICULTURAE , 2025 , 350 . |
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This study systematically investigated the optimization of total flavonoid extraction from the leaves of Bougainvillea glabra Choisy (B.glabra) and evaluated its antioxidant potential. The impact of three critical parameters (ultrasound time, heating reflux time, and ethanol concentration) on extraction efficiency was investigated using ultrasonication-assisted heat reflux extraction. A three-factor, three-level Box-Behnken design (BBD) was implemented to optimize the extraction procedure, using total flavonoid yield (TFY) as the response variable. The extracted flavonoids' antioxidant potential was measured quantitatively in vitro. The ideal conditions were 53% ethanol concentration, 10 minutes of ultrasonication, and 41 minutes of reflux. This procedure produced a TFY of (6.21 +/- 0.26)%. The extracts exhibited significant free radical scavenging action against 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical. When the concentration exceeded 1.0 mg/ mL, the scavenging rate of DPPH free radicals surpassed 84.1%. When the concentration was more than 0.6 mg/ mL, the ability of total flavonoids to scavenge ABTS free radicals was over 93.8%, which was very similar to how well vitamin C worked. This optimized protocol proves effective for flavonoid extraction, with the obtained products exhibiting significant antioxidant properties in standardized assays.
Keyword ：
antioxidant activity. (c) 2025 ACG Publications. All rights reserved antioxidant activity. (c) 2025 ACG Publications. All rights reserved Bougainvillea Bougainvillea Box-Behnken response surface methodology Box-Behnken response surface methodology total flavonoids total flavonoids ultrasound-assisted extraction ultrasound-assisted extraction
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| GB/T 7714 | Yu, Jinghui , Dong, Bin , Zhao, Yanxin et al. Process optimization and antioxidant efficacy evaluation of total flavonoids from Bougainvillea glabra leaves: an integrated approach combining ultrasonic-assisted extraction with response surface methodology [J]. | JOURNAL OF CHEMICAL METROLOGY , 2025 , 19 (1) : 103-113 . |
| MLA | Yu, Jinghui et al. "Process optimization and antioxidant efficacy evaluation of total flavonoids from Bougainvillea glabra leaves: an integrated approach combining ultrasonic-assisted extraction with response surface methodology" . | JOURNAL OF CHEMICAL METROLOGY 19 . 1 (2025) : 103-113 . |
| APA | Yu, Jinghui , Dong, Bin , Zhao, Yanxin , Fang, Rong , Fu, Shuoshuo , Sun, Qi et al. Process optimization and antioxidant efficacy evaluation of total flavonoids from Bougainvillea glabra leaves: an integrated approach combining ultrasonic-assisted extraction with response surface methodology . | JOURNAL OF CHEMICAL METROLOGY , 2025 , 19 (1) , 103-113 . |
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In the context of agricultural green transformation, the balance between the environmental footprint and economic return is a key indicator for measuring the synergy of high yields, high efficiency, and environmental friendliness in agricultural systems. However, the pathways and mechanisms for achieving this synergy in orchard systems remain unclear. Based on a three-year field experiment in Pinghe County, Fujian Province, a comprehensive evaluation framework integrating life cycle assessment (LCA) was constructed. This framework was used to systematically analyze the differences in the net ecosystem economic benefit (EEB) and environmental impact of four fertilization regimes: the conventional farming regime with no mulching (A; 1084 kg N ha(-1), 914 kg P2O5 ha(-1), and 906 kg K2O ha(-1)), the conventional farming regime with mulching (B), the optimized fertilization regime with water-fertilizer integration (C; 250 kg N ha(-1), 200 kg K2O ha(-1), 100 kg MgO ha(-1), and 400 kg CaO ha(-1)), and the optimized fertilization regime with controlled-release fertilizers (D). The results showed that regime D performed best in terms of yield, nutrient-use efficiency, and EEB, which increased by 220.5% and 297.5% compared with regime A, and reduced the input cost by CNY 63,100 similar to 69,000 hm(-2). Moreover, compared with regime A, regimes B, C, and D significantly reduced the carbon, nitrogen, and phosphorus footprints, respectively, with the carbon footprint reduced by 6.7 similar to 21.7%, 72.4 similar to 74.8%, and 71.6 similar to 76.5%; the nitrogen footprint reduced by 2.6 similar to 19.0%, 80.7 similar to 82.2%, and 80.1 similar to 83.4%; and the phosphorus footprint reduced by 15.3%, 100%, and 100%. Furthermore, the comprehensive evaluation index (CEI) is D > C > B > A. In total, the three optimized regimes balanced high yield with environmental sustainability, with the D regime showing the best performance, offering scientific support for transitioning to low-carbon, high-value orchards in smallholder systems.
Keyword ：
controlled-release fertilizer controlled-release fertilizer environmental footprint environmental footprint Guangxi pomelo Guangxi pomelo life cycle assessment life cycle assessment net ecosystem economic benefit net ecosystem economic benefit
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| GB/T 7714 | Zhang, Zetian , Gao, Guangzhao , Yu, Jinghui et al. Balancing Productivity and Environmental Sustainability in Pomelo Production Through Controlled-Release Fertilizer Optimization [J]. | AGRICULTURE-BASEL , 2025 , 15 (13) . |
| MLA | Zhang, Zetian et al. "Balancing Productivity and Environmental Sustainability in Pomelo Production Through Controlled-Release Fertilizer Optimization" . | AGRICULTURE-BASEL 15 . 13 (2025) . |
| APA | Zhang, Zetian , Gao, Guangzhao , Yu, Jinghui , Zhan, Runzhi , Yang, Hongyu , He, Zhengjia et al. Balancing Productivity and Environmental Sustainability in Pomelo Production Through Controlled-Release Fertilizer Optimization . | AGRICULTURE-BASEL , 2025 , 15 (13) . |
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本发明公开了一种促进茶园土壤固碳减排的镁肥施用装置，涉及施肥领域，包括小推车、设置于小推车顶部的储料桶、固定连接在储料桶底部的排料机构，以及固定连接在储料桶顶部的混合箱，还包括与储料桶相连接的打散机构、与混合箱相连接的搅拌机构，以及设置于储料桶与混合箱内部的分隔机构；打散机构包括贯穿储料桶侧壁并与侧壁转动连接的驱动轴，以及固定连接在驱动轴外侧壁用于带动所述驱动轴旋转的传动杆；一种促进茶园土壤固碳减排的镁肥施用装置的施用方法，包括配料、混合、下料与再混合以及施肥。本发明为一种促进茶园土壤固碳减排的镁肥施用装置及施用方法，通过将振动力转为驱动轴的驱动力，来提高行驶的稳定性。
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| GB/T 7714 | 郭梨锦 , 林蔚 , 廖鹏辉 et al. 一种促进茶园土壤固碳减排的镁肥施用装置及施用方法 : CN202411844517.9[P]. | 2024-12-16 . |
| MLA | 郭梨锦 et al. "一种促进茶园土壤固碳减排的镁肥施用装置及施用方法" : CN202411844517.9. | 2024-12-16 . |
| APA | 郭梨锦 , 林蔚 , 廖鹏辉 , 吴良泉 , 王培杰 , 何正嘉 et al. 一种促进茶园土壤固碳减排的镁肥施用装置及施用方法 : CN202411844517.9. | 2024-12-16 . |
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本发明涉及静力触探探头领域，公开了一种用于土壤碳原位评估的静力触探探头与方法，包括探头下壳体以及设于探头下壳体上端的探头上壳体，探头下壳体内设有用于检测孔径水压力的孔隙水压力探头，探头上壳体表面设有光学窗口以及用于测量土壤电学性质的电极环，而探头上壳体内设用于发射并接收漫反射光的有漫反射光发射器、控制器以及用于测量静力触探探头周围温度的温度监控器，当需要清理砂砾时，空压机启动，将高压气体输送到增压腔体中，经过增压后的气体通过连通管传递到塞环。塞环将高压气体均匀地喷射到孔隙水压力探头下端的砂砾堆积区域，高压气体的冲击力能够有效地将附着在孔隙水压力探头表面的砂砾吹散并排出。
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| GB/T 7714 | 郭梨锦 , 薛昕宇 , 沈昊鋮 et al. 一种用于土壤碳原位评估的静力触探探头与方法 : CN202510975866.2[P]. | 2025-07-16 . |
| MLA | 郭梨锦 et al. "一种用于土壤碳原位评估的静力触探探头与方法" : CN202510975866.2. | 2025-07-16 . |
| APA | 郭梨锦 , 薛昕宇 , 沈昊鋮 , 林蔚 , 王琳影 . 一种用于土壤碳原位评估的静力触探探头与方法 : CN202510975866.2. | 2025-07-16 . |
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本发明涉及种植花盆技术领域，公开了一种三角梅提根造型装置及使用方法，其结构包括花盆、无底盆、支架，本发明通过在三角梅的种植花盆上增设有无底盆，借助无底盆通过若干可拼接的扩展环组装而成，能够依据所需对三角梅根系的提根高度，进行扩展环数量的叠加，从而能够对不同高度的根系进行提根，增加了提根造型时的多变性和可适应性，且在花盆上增设有约束框，将约束框放置在花盆中心需要限制根系向下生长的区域，借助其上的孔洞能允许三角梅根系的毛细根穿过吸收花盆内部土壤的养分水分，但通过约束框能够物理阻隔主根向外深扎，迫使根系向预设的提拉区域发展，能从根源上减少无效根系生长，集中能量于造型部位，提高提根造型的效率。
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| GB/T 7714 | 郭梨锦 , 余菁慧 , 曾歆然 et al. 一种三角梅提根造型装置及使用方法 : CN202510990104.X[P]. | 2025-07-18 . |
| MLA | 郭梨锦 et al. "一种三角梅提根造型装置及使用方法" : CN202510990104.X. | 2025-07-18 . |
| APA | 郭梨锦 , 余菁慧 , 曾歆然 , 林蔚 , 何碧珠 . 一种三角梅提根造型装置及使用方法 : CN202510990104.X. | 2025-07-18 . |
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