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学者姓名：林艺芬
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Abstract ：
Harvest date is an essential impact factor for storability and chilling resistance of postharvest products. This work intended to evaluate the impacts of different harvest dates on chilling injury (CI) in Chinese olive during cold storage at 2 degrees C based on energy metabolism. The results showed that, among the harvest dates (I, III, V, and VII) selected by Chinese solar terms and TSS contents, Chinese olive at harvest date V effectively suppressed CI occurrence, and displayed the highest levels of energy charge, ADP and ATP, along with the highest NADK activity, and highest activities of H+-ATPase, Ca2+-ATPase, and Mg2+-ATPase on the membranes of plasma, vacuole, and mitochondrion. Moreover, Chinese olive at harvest date V exhibited the highest amounts of NADP and NADPH, while the lowest contents of AMP, NAD and NADH. These outcomes suggest that harvest date V (Start of winter, TSS contents 12.17 +/- 0.14 %) may be the optimal harvest date for strengthening chilling resistance via retaining superior activities of enzymes-associated to energy metabolism, thereby sustaining energy status and ion homeostasis, and consequently postponing CI occurrence in cold-stored Chinese olive.
Keyword ：
ATPase ATPase Canarium album L. Canarium album L. Chilling resistance Chilling resistance Energy state Energy state Harvest date Harvest date NADK NADK
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| GB/T 7714 | Sang, Yueying , Zhou, He , Huang, Guixia et al. Energy metabolism participates in the responses of Chinese olives at different harvest dates to chilling injury [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 . |
| MLA | Sang, Yueying et al. "Energy metabolism participates in the responses of Chinese olives at different harvest dates to chilling injury" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 231 (2026) . |
| APA | Sang, Yueying , Zhou, He , Huang, Guixia , Lin, Ziyao , Chen, Yunman , Lin, Yifen et al. Energy metabolism participates in the responses of Chinese olives at different harvest dates to chilling injury . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 . |
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Abstract ：
Longan is a highly valued tropical and subtropical cash crop with significant economic importance. However, its commercial value is often compromised by quality deterioration, particularly pulp breakdown, which is exacerbated by the hot and humid conditions of its ripening season and persistent infestations of pathogenic fungi. This research sought to explore the effects of acidic electrolyzed-oxidizing water (AEOW) on the storability of P. longanae-invaded longans at 28 +/- 1 degrees C. Result showed that, after five days of storage, AEOW treatment effectively inhibited pulp breakdown and softening in P. longanae-invaded fruit. On day 5, AEOW + P. longanae-invaded longan presented 42.22 % lower index of pulp breakdown and 62.28 % higher pulp firmness compared to the merely P. longanae-invaded longan. Additionally, in contrast to longans inoculated with P. longanae, AEOW treatment elevated the activities of disease-resistance substances synthases, chitinase, and beta-1,3-glucanase, and maintained higher levels of disease-resistance substances, thereby enhancing the fruit's disease resistance. Furthermore, AEOW treatment reduced the activities of cell wall polysaccharides-degrading enzymes in P. longanae-infected longans, thus facilitating the preservation of cell wall materials, cellulose, covalent-soluble pectin, hemicellulose, and ionic-soluble pectin contents, while decreasing the production of water-soluble pectin. The data above indicated that AEOW could effectively alleviate the decline in storability of P. longanae-invaded longans by enhancing the fruit's disease resistance and maintaining cell wall integrity, which collectively reduced the severity of P. longanae invasion.
Keyword ：
Fresh longan Fresh longan Pathogenic fungi Pathogenic fungi Pulp breakdown Pulp breakdown Pulp firmness Pulp firmness Storage property Storage property
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| GB/T 7714 | Feng, Shujuan , Zheng, Shaojie , Lin, Qin et al. Acidic electrolyzed-oxidizing water alleviates the storability decline in Phomopsis longanae Chi-infected longans by modulating the metabolisms of disease-resistance substances and cell wall polysaccharides [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 . |
| MLA | Feng, Shujuan et al. "Acidic electrolyzed-oxidizing water alleviates the storability decline in Phomopsis longanae Chi-infected longans by modulating the metabolisms of disease-resistance substances and cell wall polysaccharides" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 231 (2026) . |
| APA | Feng, Shujuan , Zheng, Shaojie , Lin, Qin , Lin, Mengshi , Zhuo, Ruiling , Lin, Yifen et al. Acidic electrolyzed-oxidizing water alleviates the storability decline in Phomopsis longanae Chi-infected longans by modulating the metabolisms of disease-resistance substances and cell wall polysaccharides . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 . |
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Abstract ：
Longan is susceptible to pericarp browning, which reduces its market value. The effects of bongkrekic acid (BKA, an energy transport inhibitor) or adenosine triphosphate (ATP) treatment on the pericarp browning, energy status, and the metabolisms of ROS, membrane lipid and phenolics in postharvest longan fruit were explored. Compared with the control group, BKA-treated longans displayed higher pericarp browning index, but lower levels of ATP and energy charge. Additionally, BKA treatment attenuated the ROS scavenging ability of longans, as evidenced by lower activities of SOD, CAT and APX, as well as lower levels of AsA and GSH, while concurrently increased O2-. production rate, H2O2 and MDA levels. Besides, BKA treatment raised the activities of PLD, lipase and LOX, as well as the amounts of PA and SFAs, while lowered the levels of PC, PI and USFAs, aggravated membrane lipid peroxidation and degradation, resulting in the disruption of membrane compartmentation, allowing PPO directly contact to phenolic substrates and causing pigment degradation. These were manifested by the reduced contents of total phenolics, flavonoid, anthocyanin, carotenoid and chlorophyll, and the declined values of chromaticity L*, a* and b*, which exacerbated longan pericarp browning. Whereas, ATP treatment presented the opposite effects. These outcomes indicated that BKA treatment-induced the suppression of energy transport could accelerate longan pericarp browning via lowering the energy level and thereafter stimulating the ROS over accumulation, membrane lipid peroxidation and degradation, thereby leading to enzymatic browning. However, ATP treatment-postponed longan pericarp browning was because ATP treatment stimulated energy transport, sustained higher levels of energy and antioxidant capacity, thereby diminishing ROS accumulation, maintaining cell membrane integrity, and restraining enzymatic browning.
Keyword ：
Adenosine triphosphate Adenosine triphosphate Bongkrekic acid Bongkrekic acid Energy level Energy level Longan pericarp browning Longan pericarp browning Membrane lipid metabolism Membrane lipid metabolism ROS metabolism ROS metabolism
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| GB/T 7714 | Li, Meiling , Lin, Hetong , Zhuo, Ruiling et al. The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 232 . |
| MLA | Li, Meiling et al. "The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 232 (2026) . |
| APA | Li, Meiling , Lin, Hetong , Zhuo, Ruiling , Li, Boqiang , Lin, Yifen , Chen, Yihui et al. The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 232 . |
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Chinese olives (Canarium album L.) are susceptible to chilling injury (CI) at inappropriate low temperature, leading to quality deterioration and loss of nutritional and commercial values. Given that cell wall polysaccharides (CWP) play a crucial role in maintaining fruit texture and quality during postharvest storage, we systematically explored the changes of quality properties, CWP metabolism, and CI development in Chinese olives during cold storage at 8 degrees C and 2 degrees C. Compared with 8 degrees C-stored Chinese olives, 2 degrees C-stored fruit presented a higher CI index, the lower levels of commercially acceptable fruit percentage, total soluble solids, total soluble sugar, sucrose, titratable acid, and vitamin C, and the higher levels of weight loss percentage and reducing sugar during cold storage. Additionally, 2 degrees C-stored fruit showed the higher levels of pectin esterase, polygalacturonase, beta-galactosidase, cellulase, xyloglucan endotransglycosylase, and water-soluble pectin, but the lower values of cell wall materials, covalent-soluble pectin, ionic-soluble pectin, cellulose, and hemicellulose. These results indicate that the inappropriate low temperature (2 degrees C) exacerbated the CI occurrence, accelerated the nutrient loss and the quality deterioration, and aggravated the CWP disassembly by stimulating the activities of cell wall-degrading enzymes in Chinese olives during cold storage.
Keyword ：
Cell wall-degrading enzymes Cell wall-degrading enzymes Cell wall polysaccharides Cell wall polysaccharides Chilling injury Chilling injury Chinese olive fruit Chinese olive fruit Quality properties Quality properties
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| GB/T 7714 | Chen, Yunman , Kuang, Xiaoyong , Lin, Ziyao et al. Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 233 . |
| MLA | Chen, Yunman et al. "Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 233 (2026) . |
| APA | Chen, Yunman , Kuang, Xiaoyong , Lin, Ziyao , Huang, Zhenlu , Huang, Guixia , Fan, Zhongqi et al. Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 233 . |
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Cellular autophagy is a self-protective mechanism that supplies nutrients to fresh produce by degrading damaged components. This research intended to investigate differences in autophagy activity and AsA-GSH cycle between 'Fuyan' and 'Dongbi' longan during pulp breakdown. The outcomes indicate that, compared to 'Fuyan' longan, 'Dongbi' longan exhibited a lower pulp breakdown index and less ROS accumulation. Additionally, 'Dongbi' longan maintained a more robust AsA-GSH cycle, characterized by higher activities of key enzymes (APX, MDHAR, DHAR, GR), higher expression levels of their encoding genes (DlAPX, DlMDHAR, DlDHAR, DlGR), and higher levels of AsA/DHA, GSH/GSSG, AsA, and GSH, alongside lower DHA and GSSG contents. Simultaneously, 'Dongbi' longan displayed higher autophagosome formation, enhanced autophagic activity, and increased expression of DlATGs (DlATG8f/8i/18g/101). These outcomes indicate that mitigated pulp breakdown in 'Dongbi' fruit may result from the synergistic interplay between AsA-GSH cycle and cellular autophagy, which provides new insights into quality maintenance of harvested longans.
Keyword ：
Ascorbate-glutathione cycle Ascorbate-glutathione cycle Autophagy Autophagy Longan fruit Longan fruit Pulp breakdown Pulp breakdown Reactive oxygen species Reactive oxygen species
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| GB/T 7714 | Sang, Yueying , Li, Shucheng , Zhuo, Ruiling et al. Comparative analysis of pulp breakdown, autophagy activity, and AsA-GSH cycle in postharvest 'Fuyan' and 'Dongbi' longan [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 233 . |
| MLA | Sang, Yueying et al. "Comparative analysis of pulp breakdown, autophagy activity, and AsA-GSH cycle in postharvest 'Fuyan' and 'Dongbi' longan" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 233 (2026) . |
| APA | Sang, Yueying , Li, Shucheng , Zhuo, Ruiling , Lin, Mengshi , Chen, Yihui , Li, Boqiang et al. Comparative analysis of pulp breakdown, autophagy activity, and AsA-GSH cycle in postharvest 'Fuyan' and 'Dongbi' longan . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 233 . |
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Recent studies have reported that energy level is an important factor influencing the quality of harvested fruit. 2, 4-Dinitrophenol (DNP) is an inhibitor of oxidative phosphorylation that reduces energy level, whereas, adenosine triphosphate (ATP) is an energy substance that can increase energy level. This work investigated the role of energy status, ion transport and respiration metabolism on the development of pulp breakdown in harvested longan treated by DNP and ATP. Results showed that, in comparison to control longan samples, DNP application worsened pulp breakdown and increased adenosine monophosphate level. Whereas, DNP reduced the levels of energy charge, ATP, adenosine diphosphate, H+ -ATPase, Ca2+-ATPase and Mg2+-ATPase. Furthermore, DNP treatment strengthened the respiration rate, increased the levels of phosphoglucose isomerase, succinate dehydrogenase, cytochrome C oxidase, ascorbic acid oxidase, polyphenol oxidase, alternative oxidase, nicotinamide adenine dinucleotide (NAD), and the reduced form of NAD (NADH), but decreased the levels of NAD kinase (NADK), nicotinamide adenine dinucleotide phosphate (NADP), the reduced form of NADP (NADPH), and glucose-6-phosphate dehydrogenase + 6-phosphogluconate dehydrogenase. These results suggest that DNP treatment induced energy deficiency, impairing ion transport capacity and disrupting the ion transport system, which damaged cell structure. This disruption further perturbed the respiration metabolism by suppressing the pentose phosphate pathway while enhancing the Embden Meyerhof Parnas, tricarboxylic acid cycle, cytochrome pathway, and alternate oxidation pathway. These changes increased the consumption of organic nutrients, thus worsening longan pulp breakdown. However, ATP treatment had the opposite effects, providing sufficient energy to maintain the balanced ion transport and respiration metabolism. This maintained cell structure, reduced nutrition consumption, and mitigated longan pulp breakdown.
Keyword ：
4-Dinitrophenol (DNP) 4-Dinitrophenol (DNP) Adenosine triphosphate (ATP) Adenosine triphosphate (ATP) Energy status Energy status Ion transport Ion transport Longan pulp breakdown Longan pulp breakdown Respiration metabolism Respiration metabolism
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| GB/T 7714 | Lin, Yifen , Chen, Jin , Lin, Yixiong et al. DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 . |
| MLA | Lin, Yifen et al. "DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 231 (2026) . |
| APA | Lin, Yifen , Chen, Jin , Lin, Yixiong , Zhang, Huili , Lin, Mengshi , Fan, Zhongqi et al. DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 . |
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Pulp breakdown is one of the primary symptoms of quality deterioration in postharvest longan fruit. As a pivotal pathogen, Phomopsis longanae Chi (P. P. longanae) ) leads to the spoilage of fresh longan. This work aimed to elucidate the influences of P. longanae infection on the metabolisms of energy and respiration in relation to longan pulp breakdown. Longan fruit were infected for 5 min with P. longanae at 10 (4) spores mL(-1 ), while the fruit dipped in distilled water were served as the control. These longans were stored for five days under the conditions of 28( degrees )C and 90 % relative humidity. The results showed that, compared with the control longans, P. longanae-- infected longans displayed a higher index of pulp breakdown, lower levels of ATP, ADP and EC, and lower activities of H+, Ca(2+ )and Mg2+-ATPase 2+-ATPase in the membranes of mitochondria, plasma and vacuoles. Besides, P. longanae-infected longans presented a higher respiration rate, higher activities of PGI, SDH, CCO, AAO, PPO and AOX, and higher levels of NAD and NADH, while showing lower activities of G-6-PDH + 6-PGDH and NADK, and lower levels of NADP and NADPH. These findings suggest that P. longanae infection aggravated the development of pulp breakdown in fresh longan, which was related to the reduced energy status and the enhanced respiratory metabolism.
Keyword ：
ATPase ATPase Energy status Energy status Longan pulp breakdown Longan pulp breakdown Pyridine nucleotides Pyridine nucleotides Respiratory pathway Respiratory pathway Respiratory terminal oxidases Respiratory terminal oxidases
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| GB/T 7714 | Zheng, Yi , Yu, Jing , Wen, Hongyi et al. Phomopsis longanae Chi infection induces changes in the metabolisms of energy and respiration in relation to longan pulp breakdown [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 . |
| MLA | Zheng, Yi et al. "Phomopsis longanae Chi infection induces changes in the metabolisms of energy and respiration in relation to longan pulp breakdown" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 219 (2025) . |
| APA | Zheng, Yi , Yu, Jing , Wen, Hongyi , Chen, Yazhen , Lin, Mengshi , Lin, Yuzhao et al. Phomopsis longanae Chi infection induces changes in the metabolisms of energy and respiration in relation to longan pulp breakdown . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 . |
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This work investigated how salicylic acid (SA) treatment enhanced the storage quality of longan fruit by affecting its respiration and energy metabolism. Compared to control longan, SA-treated longan exhibited higher levels of commercially acceptable fruit rate, sucrose, vitamin C, TSS, and total soluble sugar, but lower levels of fruit disease index, pulp breakdown index, weight loss, TA, and reducing sugar. Additionally, SA treatment resulted in lower fruit respiration rate, lower activities of the critical enzymes involved in respiratory pathways (PGI, SDH, CCO, AAO), lower values of NADH and NAD, higher activities of enzymes (NADK, G-6-PDH + 6-PGDH), and higher amounts of NADPH and NADP. Besides, SA-treated longan showed higher levels of ATP, ADP, and energy charge, but lower AMP level, along with higher activities of H+-ATPase, Ca2+-ATPase, and Mg2+-ATPase in mitochondrial, plasma, and vacuolar membranes. These data indicated that SA treatment-maintained the storage attributes of postharvest longan was due to the weakened respiratory intensity, the reduced consumption of nutrients, the inhibited respiratory pathways of EMP-TCA cycle, and the enhanced respiratory pathway of PPP. Additionally, SA treatment retained higher levels of ATPase activity and energy status to maintain cellular ionic balance and protect membrane integrity, ultimately improved the storability of fresh longan during storage.
Keyword ：
Energy metabolism Energy metabolism Longan fruit Longan fruit Quality attributes Quality attributes Respiratory metabolism Respiratory metabolism Salicylic acid Salicylic acid Storability Storability
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| GB/T 7714 | Zhang, Huili , Shan, Tingting , Chen, Yang et al. Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 . |
| MLA | Zhang, Huili et al. "Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 219 (2025) . |
| APA | Zhang, Huili , Shan, Tingting , Chen, Yang , Wen, Hongyi , Lin, Mengshi , Fan, Zhongqi et al. Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 . |
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Compared to control longan, on day 6, DCC-treated longan presented 18 %, 2 %, 15 %, 34 %, 21 %, 26 % and 10 % higher levels of pulp breakdown index, fruit respiration rate, PGI, SDH, NAD, NADH and QH2, respectively. Meanwhile, DCC-treated longan displayed 25 %, 5 %, 8 % and 12 % higher activities of respiratory terminal oxidases including CCO, AOX, PPO and AAO, respectively. However, DCC-treated longan presented 9 %, 27 %, 24 %, 25 % and 16 % lower levels of NADK, G-6-PDH + 6-PGDH, NADP, NADPH and Q, respectively. These data indicate that DCC-hastened longan pulp breakdown occurrence was owing to the diminished PPP, and the elevated EMP, TCA cycle and CCP, thereby leading to an increased respiration rate. However, DS treatment displayed contrary effects, indicating DS restrained longan pulp breakdown occurrence through diminishing the respiration metabolism and depressing the respiration rate of fresh longan.
Keyword ：
Dimocarpus longan Lour. Dimocarpus longan Lour. Fruit quality Fruit quality Pyridine nucleotides Pyridine nucleotides Respiratory metabolic pathway Respiratory metabolic pathway Respiratory terminal oxidases Respiratory terminal oxidases
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| GB/T 7714 | Zheng, Yi , Lin, Yixiong , Wen, Hongyi et al. The role of respiration metabolism in dicyclohexylcarbodiimide and disodium succinate regulating the pulp breakdown occurrence of fresh longan (Dimocarpus longan Lour.) during storage [J]. | FOOD CHEMISTRY-X , 2025 , 27 . |
| MLA | Zheng, Yi et al. "The role of respiration metabolism in dicyclohexylcarbodiimide and disodium succinate regulating the pulp breakdown occurrence of fresh longan (Dimocarpus longan Lour.) during storage" . | FOOD CHEMISTRY-X 27 (2025) . |
| APA | Zheng, Yi , Lin, Yixiong , Wen, Hongyi , Sang, Yueying , Lin, Mengshi , Fan, Zhongqi et al. The role of respiration metabolism in dicyclohexylcarbodiimide and disodium succinate regulating the pulp breakdown occurrence of fresh longan (Dimocarpus longan Lour.) during storage . | FOOD CHEMISTRY-X , 2025 , 27 . |
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The impacts of Bacillus amyloliquefaciens LY-1 culture broth (BLCB) on the fruit storage properties and reactive oxygen species (ROS) metabolism of postharvest 'Wuye' litchis were studied. In comparation with control fruit, BLCB-treated litchis showed a lower fruit disease index, a higher rate of commercially acceptable fruit, higher amounts of pericarp pigments (total phenolics, anthocyanin, carotenoid, chlorophyll and flavonoid), higher chromaticity C, a*, b* and L* values but lower hue angle h degrees of fruit surface. Additionally, BLCB-treated litchis exhibited lower malonaldehyde (MDA) accumulation and superoxide anion radical (O2.-) production rate, higher APX, CAT and SOD activities, higher GSH and AsA amounts, higher reducing power, and higher ability of scavenging DPPH radical. Furthermore, the pericarp browning index and fruit disease index were positively correlated with O2.- production rate. These findings suggested that BLCB treatment increased the storability of postharvest litchi fruit through enhancing scavenging capacity of ROS and inhibiting overaccumulation of ROS.
Keyword ：
Appearance color Appearance color Biocontrol bacteria Biocontrol bacteria Disease occurrence Disease occurrence Litchi chinensis Sonn. Litchi chinensis Sonn. Quality attributes Quality attributes Storability Storability
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| GB/T 7714 | Wang, Hui , Yuan, Junhui , Wu, Yijing et al. Bacillus amyloliquefaciens LY-1 culture broth enhances the storage properties of fresh litchi through acting on ROS metabolism [J]. | FOOD CHEMISTRY , 2025 , 480 . |
| MLA | Wang, Hui et al. "Bacillus amyloliquefaciens LY-1 culture broth enhances the storage properties of fresh litchi through acting on ROS metabolism" . | FOOD CHEMISTRY 480 (2025) . |
| APA | Wang, Hui , Yuan, Junhui , Wu, Yijing , Wen, Yifan , Lin, Yifen , Chen, Yihui et al. Bacillus amyloliquefaciens LY-1 culture broth enhances the storage properties of fresh litchi through acting on ROS metabolism . | FOOD CHEMISTRY , 2025 , 480 . |
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