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学者姓名:范中奇

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DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism SCIE
期刊论文 | 2026 , 231 | POSTHARVEST BIOLOGY AND TECHNOLOGY
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Abstract :

Recent studies have reported that energy level is an important factor influencing the quality of harvested fruit. 2, 4-Dinitrophenol (DNP) is an inhibitor of oxidative phosphorylation that reduces energy level, whereas, adenosine triphosphate (ATP) is an energy substance that can increase energy level. This work investigated the role of energy status, ion transport and respiration metabolism on the development of pulp breakdown in harvested longan treated by DNP and ATP. Results showed that, in comparison to control longan samples, DNP application worsened pulp breakdown and increased adenosine monophosphate level. Whereas, DNP reduced the levels of energy charge, ATP, adenosine diphosphate, H+ -ATPase, Ca2+-ATPase and Mg2+-ATPase. Furthermore, DNP treatment strengthened the respiration rate, increased the levels of phosphoglucose isomerase, succinate dehydrogenase, cytochrome C oxidase, ascorbic acid oxidase, polyphenol oxidase, alternative oxidase, nicotinamide adenine dinucleotide (NAD), and the reduced form of NAD (NADH), but decreased the levels of NAD kinase (NADK), nicotinamide adenine dinucleotide phosphate (NADP), the reduced form of NADP (NADPH), and glucose-6-phosphate dehydrogenase + 6-phosphogluconate dehydrogenase. These results suggest that DNP treatment induced energy deficiency, impairing ion transport capacity and disrupting the ion transport system, which damaged cell structure. This disruption further perturbed the respiration metabolism by suppressing the pentose phosphate pathway while enhancing the Embden Meyerhof Parnas, tricarboxylic acid cycle, cytochrome pathway, and alternate oxidation pathway. These changes increased the consumption of organic nutrients, thus worsening longan pulp breakdown. However, ATP treatment had the opposite effects, providing sufficient energy to maintain the balanced ion transport and respiration metabolism. This maintained cell structure, reduced nutrition consumption, and mitigated longan pulp breakdown.

Keyword :

4-Dinitrophenol (DNP) 4-Dinitrophenol (DNP) Adenosine triphosphate (ATP) Adenosine triphosphate (ATP) Energy status Energy status Ion transport Ion transport Longan pulp breakdown Longan pulp breakdown Respiration metabolism Respiration metabolism

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GB/T 7714 Lin, Yifen , Chen, Jin , Lin, Yixiong et al. DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 .
MLA Lin, Yifen et al. "DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 231 (2026) .
APA Lin, Yifen , Chen, Jin , Lin, Yixiong , Zhang, Huili , Lin, Mengshi , Fan, Zhongqi et al. DNP and ATP treatment regulate longan pulp breakdown by acting on energy status, ion transport and respiration metabolism . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 231 .
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The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning SCIE
期刊论文 | 2026 , 232 | POSTHARVEST BIOLOGY AND TECHNOLOGY
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Abstract :

Longan is susceptible to pericarp browning, which reduces its market value. The effects of bongkrekic acid (BKA, an energy transport inhibitor) or adenosine triphosphate (ATP) treatment on the pericarp browning, energy status, and the metabolisms of ROS, membrane lipid and phenolics in postharvest longan fruit were explored. Compared with the control group, BKA-treated longans displayed higher pericarp browning index, but lower levels of ATP and energy charge. Additionally, BKA treatment attenuated the ROS scavenging ability of longans, as evidenced by lower activities of SOD, CAT and APX, as well as lower levels of AsA and GSH, while concurrently increased O2-. production rate, H2O2 and MDA levels. Besides, BKA treatment raised the activities of PLD, lipase and LOX, as well as the amounts of PA and SFAs, while lowered the levels of PC, PI and USFAs, aggravated membrane lipid peroxidation and degradation, resulting in the disruption of membrane compartmentation, allowing PPO directly contact to phenolic substrates and causing pigment degradation. These were manifested by the reduced contents of total phenolics, flavonoid, anthocyanin, carotenoid and chlorophyll, and the declined values of chromaticity L*, a* and b*, which exacerbated longan pericarp browning. Whereas, ATP treatment presented the opposite effects. These outcomes indicated that BKA treatment-induced the suppression of energy transport could accelerate longan pericarp browning via lowering the energy level and thereafter stimulating the ROS over accumulation, membrane lipid peroxidation and degradation, thereby leading to enzymatic browning. However, ATP treatment-postponed longan pericarp browning was because ATP treatment stimulated energy transport, sustained higher levels of energy and antioxidant capacity, thereby diminishing ROS accumulation, maintaining cell membrane integrity, and restraining enzymatic browning.

Keyword :

Adenosine triphosphate Adenosine triphosphate Bongkrekic acid Bongkrekic acid Energy level Energy level Longan pericarp browning Longan pericarp browning Membrane lipid metabolism Membrane lipid metabolism ROS metabolism ROS metabolism

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GB/T 7714 Li, Meiling , Lin, Hetong , Zhuo, Ruiling et al. The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 232 .
MLA Li, Meiling et al. "The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 232 (2026) .
APA Li, Meiling , Lin, Hetong , Zhuo, Ruiling , Li, Boqiang , Lin, Yifen , Chen, Yihui et al. The changes of antioxidant system, membrane integrity and phenolics metabolism participate in bongkrekic acid and adenosine triphosphate-mediated energy status regulating longan pericarp browning . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 232 .
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Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development SCIE
期刊论文 | 2026 , 233 | POSTHARVEST BIOLOGY AND TECHNOLOGY
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Abstract :

Chinese olives (Canarium album L.) are susceptible to chilling injury (CI) at inappropriate low temperature, leading to quality deterioration and loss of nutritional and commercial values. Given that cell wall polysaccharides (CWP) play a crucial role in maintaining fruit texture and quality during postharvest storage, we systematically explored the changes of quality properties, CWP metabolism, and CI development in Chinese olives during cold storage at 8 degrees C and 2 degrees C. Compared with 8 degrees C-stored Chinese olives, 2 degrees C-stored fruit presented a higher CI index, the lower levels of commercially acceptable fruit percentage, total soluble solids, total soluble sugar, sucrose, titratable acid, and vitamin C, and the higher levels of weight loss percentage and reducing sugar during cold storage. Additionally, 2 degrees C-stored fruit showed the higher levels of pectin esterase, polygalacturonase, beta-galactosidase, cellulase, xyloglucan endotransglycosylase, and water-soluble pectin, but the lower values of cell wall materials, covalent-soluble pectin, ionic-soluble pectin, cellulose, and hemicellulose. These results indicate that the inappropriate low temperature (2 degrees C) exacerbated the CI occurrence, accelerated the nutrient loss and the quality deterioration, and aggravated the CWP disassembly by stimulating the activities of cell wall-degrading enzymes in Chinese olives during cold storage.

Keyword :

Cell wall-degrading enzymes Cell wall-degrading enzymes Cell wall polysaccharides Cell wall polysaccharides Chilling injury Chilling injury Chinese olive fruit Chinese olive fruit Quality properties Quality properties

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GB/T 7714 Chen, Yunman , Kuang, Xiaoyong , Lin, Ziyao et al. Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 233 .
MLA Chen, Yunman et al. "Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 233 (2026) .
APA Chen, Yunman , Kuang, Xiaoyong , Lin, Ziyao , Huang, Zhenlu , Huang, Guixia , Fan, Zhongqi et al. Changes in quality properties and cell wall polysaccharides metabolism of Chinese olives during chilling injury development . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2026 , 233 .
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Comparative analysis in postharvest storability, quality attributes, antioxidant activity, and disease defense systems in 'Fuyan' and 'Dongbi' longans during storage SCIE
期刊论文 | 2025 , 223 | POSTHARVEST BIOLOGY AND TECHNOLOGY
WoS CC Cited Count: 3
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Abstract :

This research aimed to compare the fruit storability and quality differences between the 'Fuyan' and 'Dongbi' varieties of longan (Dimocarpus longan Lour.) by assaying their antioxidant system and disease defense systems. The results showed that, compared to 'Fuyan' fruit, during storage, 'Dongbi' fruit exhibited superior storability and appearance, characterized by lower levels of fruit disease, pulp breakdown, pericarp browning, and weight loss, alongside higher pericarp color, brightness, pigments, pulp nutrient content, and commercial acceptability of fruit. Additionally, 'Dongbi' longans exhibited lower ROS accumulation and MDA level, higher levels of antioxidants (AsA, GSH), antioxidant enzymes (SOD, CAT, APX), reducing power, and DPPH center dot scavenging ability. Besides, 'Dongbi' longans retained higher levels of secondary metabolites (lignin, total phenolics, flavonoid), PRMEs (PAL, C4H, 4-CL, CAD, POD), and PRs (CHI, GLU). Therefore, the enhanced storage stability of 'Dongbi' longans may be attributed to the synergistic effects of an improved antioxidant system, phenylpropanoid pathway, and PRs.

Keyword :

Dimocarpus longan Lour. Dimocarpus longan Lour. Pericarp browning Pericarp browning Phenylpropanoid metabolism Phenylpropanoid metabolism Pulp breakdown Pulp breakdown Reactive oxygen species (ROS) Reactive oxygen species (ROS) ROS metabolism ROS metabolism

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GB/T 7714 Sang, Yueying , Lin, Lijuan , Wen, Hongyi et al. Comparative analysis in postharvest storability, quality attributes, antioxidant activity, and disease defense systems in 'Fuyan' and 'Dongbi' longans during storage [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 223 .
MLA Sang, Yueying et al. "Comparative analysis in postharvest storability, quality attributes, antioxidant activity, and disease defense systems in 'Fuyan' and 'Dongbi' longans during storage" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 223 (2025) .
APA Sang, Yueying , Lin, Lijuan , Wen, Hongyi , Lin, Yixiong , Lin, Mengshi , Chen, Yihui et al. Comparative analysis in postharvest storability, quality attributes, antioxidant activity, and disease defense systems in 'Fuyan' and 'Dongbi' longans during storage . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 223 .
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Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy SCIE
期刊论文 | 2025 , 219 | POSTHARVEST BIOLOGY AND TECHNOLOGY
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Abstract :

This work investigated how salicylic acid (SA) treatment enhanced the storage quality of longan fruit by affecting its respiration and energy metabolism. Compared to control longan, SA-treated longan exhibited higher levels of commercially acceptable fruit rate, sucrose, vitamin C, TSS, and total soluble sugar, but lower levels of fruit disease index, pulp breakdown index, weight loss, TA, and reducing sugar. Additionally, SA treatment resulted in lower fruit respiration rate, lower activities of the critical enzymes involved in respiratory pathways (PGI, SDH, CCO, AAO), lower values of NADH and NAD, higher activities of enzymes (NADK, G-6-PDH + 6-PGDH), and higher amounts of NADPH and NADP. Besides, SA-treated longan showed higher levels of ATP, ADP, and energy charge, but lower AMP level, along with higher activities of H+-ATPase, Ca2+-ATPase, and Mg2+-ATPase in mitochondrial, plasma, and vacuolar membranes. These data indicated that SA treatment-maintained the storage attributes of postharvest longan was due to the weakened respiratory intensity, the reduced consumption of nutrients, the inhibited respiratory pathways of EMP-TCA cycle, and the enhanced respiratory pathway of PPP. Additionally, SA treatment retained higher levels of ATPase activity and energy status to maintain cellular ionic balance and protect membrane integrity, ultimately improved the storability of fresh longan during storage.

Keyword :

Energy metabolism Energy metabolism Longan fruit Longan fruit Quality attributes Quality attributes Respiratory metabolism Respiratory metabolism Salicylic acid Salicylic acid Storability Storability

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GB/T 7714 Zhang, Huili , Shan, Tingting , Chen, Yang et al. Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 .
MLA Zhang, Huili et al. "Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 219 (2025) .
APA Zhang, Huili , Shan, Tingting , Chen, Yang , Wen, Hongyi , Lin, Mengshi , Fan, Zhongqi et al. Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 .
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Comparative transcriptomic analysis reveals the reactive oxygen species metabolism involving in melatonin-alleviated chilling injury in postharvest banana fruit SCIE
期刊论文 | 2025 , 222 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
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Abstract :

Banana fruit is susceptible to chilling injury (CI) during cold storage, which causes quality deterioration and commodity value decline. Here, the effects of melatonin on CI alleviation and reactive oxygen species (ROS) metabolism in postharvest banana were studied. The results displayed that 500 mu mol L-1 melatonin treatment effectively inhibited CI development in cold-stored banana during the 96 h of storage. Additionally, lower O2.productive rate and H2O2 content, but higher contents of ascorbic acid (AsA) and glutathione (GSH) were found in melatonin-treated banana, compared with control fruits. Also, melatonin inhibited the declined activities of antioxidant enzymes. Moreover, a total of 1858 and 330 differentially expressed genes (DEGs) were identified between the comparison of CK48h (control bananas at 48 h) and MT48h (melatonin-treated bananas at 48 h), and CK96h (control bananas at 96 h) and MT96h (melatonin-treated bananas at 96 h) through comparative transcriptome analysis of banana fruit, respectively. Further KEGG displayed that DEGs were enriched in secondary metabolites biosynthesis, MAPK signaling pathway-plant and plant hormone signal transduction. The transcriptome expression profiling and RT-qPCR exhibited melatonin treatment improved the expressions of antioxidant enzyme genes (MaSOD, MaCAT, MaAPX and MaGR) but inhibited the expression of ROS production gene (MaRBOH). Collectively, these findings provide a comprehensive view of ROS metabolism associated with the melatonin-alleviated CI in cold-stored banana fruit.

Keyword :

Antioxidant enzyme Antioxidant enzyme Banana Banana Chilling injury Chilling injury ROS metabolism ROS metabolism Transcriptome Transcriptome

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GB/T 7714 Wu, Yanting , Bai, Lijuan , Dai, Xiaoze et al. Comparative transcriptomic analysis reveals the reactive oxygen species metabolism involving in melatonin-alleviated chilling injury in postharvest banana fruit [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 .
MLA Wu, Yanting et al. "Comparative transcriptomic analysis reveals the reactive oxygen species metabolism involving in melatonin-alleviated chilling injury in postharvest banana fruit" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 222 (2025) .
APA Wu, Yanting , Bai, Lijuan , Dai, Xiaoze , Ba, Liangjie , Wan, Jiahui , Liang, Weiqi et al. Comparative transcriptomic analysis reveals the reactive oxygen species metabolism involving in melatonin-alleviated chilling injury in postharvest banana fruit . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 .
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Integrative transcriptomic and metabolomic analyses reveal the mechanisms behind acidity differences in the pulp of fresh longan cv. 'Fuyan' and 'Dongbi' during storage SCIE
期刊论文 | 2025 , 223 | POSTHARVEST BIOLOGY AND TECHNOLOGY
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Abstract :

To understand the underlying mechanism of the organic acid accumulation of postharvest longan fruit, integrative analyses of transcriptome and metabolome were performed in the fruit of two longan varieties ('Fuyan' and 'Dongbi') with different storability. Compared with 'Dongbi' longan, the 'Fuyan' cultivar exhibited higher fruit respiration rate, pulp breakdown index, and TA content, but a lower TSS content during storage. Targeted metabolomic analysis revealed that, in comparison to 'Dongbi' longan, during storage, 'Fuyan' longan accumulated higher levels of four organic acids, including 3-phenyllactic acid, lactate, succinic acid, and oxaloacetate. Transcriptomic analysis showed that DEGs between 'Fuyan' and 'Dongbi' longans were primarily enriched in respiration metabolism pathways, including glycolysis, gluconeogenesis, and the TCA cycle. Additionally, WGCNA identified hub genes involved in these metabolic pathways. RT-qPCR analysis indicated that the expression of related genes (DlLDH, DlACL, DlPEPC, DlMDH, DlAST and DlSCS) was upregulated in 'Fuyan' longan compared with 'Dongbi' longan during storage. These findings suggest that the increase in longan pulp acidity is attributed to organic acid accumulation and an enhanced respiration metabolism, resulting in the poor storability of 'Fuyan' longan during storage.

Keyword :

Longan fruit Longan fruit Metabolome Metabolome Organic acid Organic acid Pulp acidity Pulp acidity Respiration Respiration Transcriptome Transcriptome

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GB/T 7714 Zeng, Lingzhen , Sang, Yueying , Li, Shucheng et al. Integrative transcriptomic and metabolomic analyses reveal the mechanisms behind acidity differences in the pulp of fresh longan cv. 'Fuyan' and 'Dongbi' during storage [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 223 .
MLA Zeng, Lingzhen et al. "Integrative transcriptomic and metabolomic analyses reveal the mechanisms behind acidity differences in the pulp of fresh longan cv. 'Fuyan' and 'Dongbi' during storage" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 223 (2025) .
APA Zeng, Lingzhen , Sang, Yueying , Li, Shucheng , Lin, Mengshi , Lin, Yifen , Wang, Hui et al. Integrative transcriptomic and metabolomic analyses reveal the mechanisms behind acidity differences in the pulp of fresh longan cv. 'Fuyan' and 'Dongbi' during storage . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 223 .
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γ-Aminobutyric acid treatment reduces the chilling injury and quality deterioration of Chinese olives during cold storage through regulating the metabolisms of respiration and energy SCIE
期刊论文 | 2025 , 342 | SCIENTIA HORTICULTURAE
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Chilling injury (CI) is one of the main symptoms of quality deterioration in cold-stored Chinese olives, leading to the rapid declines of nutrition and commercial value. This work explored the efficacy of 1.0 mM gamma-aminobutyric acid (GABA) treatment on the respiratory and energy metabolisms in Chinese olives during cold storage at 2 degrees C and its relationship with CI development and quality attributes. The Chinese olives were immersed in distilled water and 1.0 mM GABA solution for 10 min, respectively. Compared with the control samples, the GABA-treated Chinese olives presented lower levels of fruit CI index and weight loss percentage, and lower contents of pericarp carotenoid and pulp reducing sugar. Conversely, the GABA-treated samples showed a higher percentage of the commercially acceptable fruit, a higher pericarp h degrees value, higher levels of pericarp chlorophyll and pulp total soluble solids, titratable acidity, tannin, vitamin C, total soluble sugar, and sucrose. Additionally, the GABAtreated samples showed lower respiration rate, lower activities of phosphoglucose isomerase, succinate dehydrogenase, cytochrome C oxidase, and ascorbic acid oxidase, lower values of nicotinamide adenine dinucleotide (NAD) and the reduced form of NAD (NADH), and lower content of adenosine monophosphate, but higher activities of glucose-6-phosphate dehydrogenase + 6-phosphogluconate dehydrogenase, nicotinamide adenine dinucleotide kinase, H+, Ca2+, and Mg2+-ATPase, higher values of nicotinamide adenine dinucleotide phosphate (NADP) and the reduced form of NADP (NADPH), and higher contents of adenosine triphosphate, adenosine diphosphate, and energy charge. These findings elucidate that the alleviating effects of GABA on the CI occurrence and quality deterioration in Chinese olives during cold storage are related to the inhibition of respiratory metabolism and the maintenance of a higher energy status.

Keyword :

ATPase ATPase Canarium album Lour. Canarium album Lour. Cold storage Cold storage Energy status Energy status Nutritional composition Nutritional composition Pyridine nucleotides Pyridine nucleotides

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GB/T 7714 Chen, Yunman , Lin, Bin , Wen, Yifan et al. γ-Aminobutyric acid treatment reduces the chilling injury and quality deterioration of Chinese olives during cold storage through regulating the metabolisms of respiration and energy [J]. | SCIENTIA HORTICULTURAE , 2025 , 342 .
MLA Chen, Yunman et al. "γ-Aminobutyric acid treatment reduces the chilling injury and quality deterioration of Chinese olives during cold storage through regulating the metabolisms of respiration and energy" . | SCIENTIA HORTICULTURAE 342 (2025) .
APA Chen, Yunman , Lin, Bin , Wen, Yifan , Sang, Yueying , Lin, Mengshi , Li, Shucheng et al. γ-Aminobutyric acid treatment reduces the chilling injury and quality deterioration of Chinese olives during cold storage through regulating the metabolisms of respiration and energy . | SCIENTIA HORTICULTURAE , 2025 , 342 .
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MaERF9 and MaERF113 transcription factors involve in chilling injury development by regulating membrane lipid metabolism of postharvest banana fruit SCIE
期刊论文 | 2025 , 219 | POSTHARVEST BIOLOGY AND TECHNOLOGY
WoS CC Cited Count: 2
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Abstract :

Banana is a cold-sensitive fruit, which exhibits vulnerability to chilling injury (CI), causing browning, pitting, even abnormal ripening, resulting in quality deterioration and economic loss. CI development of banana fruit was caused by membrane integrity damage and was related to the enzymatic and genetic manipulation of membrane lipid metabolism pathway. The involvement of Ethylene Response Factor (ERF) transcription factors (TFs) in membrane lipid-mediated CI development of cold-sensitive fruit, such as banana, remains largely unknown. In this study, we found that cold treatment increased CI index, cell membrane permeability and MDA content, decreased hue angle (h degrees) value. The expressions of membrane lipids metabolism genes, including MaDGK, MaPLA, MaLipase and MaLOX, were upregulated by cold treatment. Also, two banana ERF TFs, designated as MaERF9 and MaERF113, were induced by cold environment and displayed transactivation activities. Additionally, the electrophoretic mobility shift assay (EMSA) and transient expression analysis showed that MaERF9 and MaERF113 directly bound to the GCC-box elements in the promoters of MaDGK, MaPLA, MaLipase and MaLOX, and activated their expressions. These findings imply that MaERF9 and MaERF113 involved in CI development of banana fruit, via affecting membrane lipid metabolism. Collectively, our results provide new insights into the transcriptional regulation network regulating CI development of banana fruit.

Keyword :

Banana fruit Banana fruit Chilling injury Chilling injury ERF transcription factors ERF transcription factors Membrane lipid metabolism Membrane lipid metabolism Transcriptional regulation Transcriptional regulation

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GB/T 7714 Bai, Lijuan , Wu, Yanting , Lin, Hetong et al. MaERF9 and MaERF113 transcription factors involve in chilling injury development by regulating membrane lipid metabolism of postharvest banana fruit [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 .
MLA Bai, Lijuan et al. "MaERF9 and MaERF113 transcription factors involve in chilling injury development by regulating membrane lipid metabolism of postharvest banana fruit" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 219 (2025) .
APA Bai, Lijuan , Wu, Yanting , Lin, Hetong , Su, Wenbing , Fan, Zhongqi . MaERF9 and MaERF113 transcription factors involve in chilling injury development by regulating membrane lipid metabolism of postharvest banana fruit . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 219 .
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Combined analyses of metabolomics and transcriptomics reveal the mechanism of DNP and ATP regulating the metabolisms of organic acids in pulp of longan fruit during storage SCIE
期刊论文 | 2025 , 224 | POSTHARVEST BIOLOGY AND TECHNOLOGY
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The quality deterioration in pulp of postharvest longan fruit is related to the increase in acidity during storage. However, the main organic acids and their regulatory mechanisms are still unrevealed. This study explored the impacts of 2,4-dinitrophenol (DNP) and adenosine triphosphate (ATP) treatments on the energy level and their influences on titratable acid (TA) contents in longan pulp. Compared with the control samples, lower levels of pulp energy charge, ATP and total soluble solids (TSS), but higher fruit respiration rate, pulp breakdown index and TA content were found in DNP-treated longans. Moreover, higher levels of four organic acids (fumaric acid, lactate, succinic acid, and oxaloacetate) were presented in DNP-treated samples through the analysis of targeted metabolome. However, the ATP treatment showed the opposite effects. The transcriptome analysis showed that differentially expressed genes (DEGs) among the three treatment groups were predominantly enriched in respiration metabolism pathways, including glycolysis, gluconeogenesis and tricarboxylic acid (TCA) cycle. In addition, weighted gene co-expression network analysis (WGCNA) identified four genes (DlSDH, DlLDH, DlSCS, and DlMDH) associated with the aforementioned metabolic pathways. RT-qPCR results exhibited that, during storage, these four genes in longan pulp were upregulated by DNP treatment and downregulated by ATP treatment. Therefore, energy deficit could promote the expression of DlSDH, DlLDH, DlSCS, and DlMDH, enhancing the metabolisms of glycolysis, gluconeogenesis, and TCA cycle, thereby accelerating the increase of organic acids in pulp of fresh longan during storage. This work revealed the main organic acids, the metabolic pathways, and the key genes involved in the increased acidity in pulp of postharvest longan fruit, as well as expanded our knowledge of energy regulating longan fruit quality and its relationship with the metabolism of organic acids.

Keyword :

Differentially expressed genes Differentially expressed genes Dimocarpus longan Lour. Dimocarpus longan Lour. Energy status Energy status Organic acid metabolism Organic acid metabolism Titratable acid Titratable acid WGCNA WGCNA

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GB/T 7714 Li, Zhe , Lin, Hetong , Zeng, Lingzhen et al. Combined analyses of metabolomics and transcriptomics reveal the mechanism of DNP and ATP regulating the metabolisms of organic acids in pulp of longan fruit during storage [J]. | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 224 .
MLA Li, Zhe et al. "Combined analyses of metabolomics and transcriptomics reveal the mechanism of DNP and ATP regulating the metabolisms of organic acids in pulp of longan fruit during storage" . | POSTHARVEST BIOLOGY AND TECHNOLOGY 224 (2025) .
APA Li, Zhe , Lin, Hetong , Zeng, Lingzhen , Li, Shucheng , Sang, Yueying , Fan, Zhongqi et al. Combined analyses of metabolomics and transcriptomics reveal the mechanism of DNP and ATP regulating the metabolisms of organic acids in pulp of longan fruit during storage . | POSTHARVEST BIOLOGY AND TECHNOLOGY , 2025 , 224 .
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