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学者姓名：刘忱
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Abstract ：
BackgroundAntimicrobial resistance poses a substantial and growing threat to global health. While antibiotic resistance genes (ARGs) are tracked most closely in clinical settings, their spread remains poorly understood in non-clinical environments. Mitigating the spread of ARGs in non-clinical contexts such as soil could limit their enrichment in food webs.ResultsMulti-omics (involving metagenomics, metatranscriptomics, viromics, and metabolomics) and direct experimentation show that targeting keystone bacterial taxa by phages can limit ARG maintenance and dissemination in natural soil environments. Based on the metagenomic analysis, we first show that phages from activated sludge can regulate soil microbiome composition and function in terms of reducing ARG abundances and changing the bacterial community composition. This effect was mainly driven by a reduction in the abundance and activity of Streptomyces genus, which is well known for encoding both antibiotic resistance and synthesis genes. To validate the significance of this keystone species for the loss of ARGs, we enriched phage consortia specific to Streptomyces and tested their effect on ARG abundances on 48 soil samples collected across China. We observed a consistent reduction in ARG abundances across all soils, confirming that Streptomyces-enriched phages could predictably change the soil microbiome resistome and mitigate the prevalence of ARGs. This study highlights that phages can be used as ecosystem engineers to control the spread of antibiotic resistance in the environment.ConclusionOur study demonstrates that some bacterial keystone taxa are critical for ARG maintenance and dissemination in soil microbiomes, and opens new ecological avenues for microbiome modification and resistome control. This study advances our understanding of how metagenomics-informed phage consortia can be used to predictably regulate soil microbiome composition and functioning by targeting keystone bacterial taxa.3cmU8RStwhfZ94ahX7b_G7Video AbstractConclusionOur study demonstrates that some bacterial keystone taxa are critical for ARG maintenance and dissemination in soil microbiomes, and opens new ecological avenues for microbiome modification and resistome control. This study advances our understanding of how metagenomics-informed phage consortia can be used to predictably regulate soil microbiome composition and functioning by targeting keystone bacterial taxa.3cmU8RStwhfZ94ahX7b_G7Video Abstract
Keyword ：
Antibiotic resistance Antibiotic resistance Farmland soil Farmland soil Keystone taxa Keystone taxa Metagenomics and viromics Metagenomics and viromics Phage community Phage community
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| GB/T 7714 | Liao, Hanpeng , Wen, Chang , Huang, Dan et al. Harnessing phage consortia to mitigate the soil antibiotic resistome by targeting keystone taxa Streptomyces [J]. | MICROBIOME , 2025 , 13 (1) . |
| MLA | Liao, Hanpeng et al. "Harnessing phage consortia to mitigate the soil antibiotic resistome by targeting keystone taxa Streptomyces" . | MICROBIOME 13 . 1 (2025) . |
| APA | Liao, Hanpeng , Wen, Chang , Huang, Dan , Liu, Chen , Gao, Tian , Du, Qiyao et al. Harnessing phage consortia to mitigate the soil antibiotic resistome by targeting keystone taxa Streptomyces . | MICROBIOME , 2025 , 13 (1) . |
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Abstract ：
抗生素耐药性的传播已严重威胁全球公共健康,近年来研究发现非抗生素类化学物质也能促进细菌耐药性的产生与传播.然而,除草剂的大量使用是否对细菌耐药性产生影响却鲜见报道.本文以模式菌株大肠杆菌(Escherichia coli DH5α)为研究对象,探究3种常用除草剂(草甘膦、草铵膦和麦草畏)对E. coli DH5α耐药性的影响.结果表明,在土壤环境浓度暴露下,除草剂处理可以改变大肠杆菌对抗生素的敏感性,显著提高大肠杆菌对庆大霉素的耐药能力,其中不同除草剂对细菌耐药性影响效果为:草甘膦>麦草畏>草铵膦.除草剂暴露30 d后,大肠杆菌突变菌株同时增强了对四环素、氯霉素和氨基糖苷类抗生素的耐药能力,其中链霉素的最小抑制浓度提高19.8倍.全基因组测序发现,除草剂可以诱导膜蛋白(omp F、pap C)、菌毛蛋白(yra H)和核糖体(rps L)等与抗生素耐药性相关的基因突变,从而增强其耐药能力.以上结果表明除草剂通过增加染色体基因突变增强细菌耐药能力,从而促进耐药基因在环境中传播的潜在风险.
Keyword ：
基因突变 基因突变 大肠杆菌 大肠杆菌 抗生素耐药性 抗生素耐药性 突变频率 突变频率 除草剂 除草剂
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| GB/T 7714 | Li, Xi , Liao, Han-Peng , Cui, Peng et al. 3种常用除草剂对细菌抗生素耐药性的影响 [J]. | 环境科学 , 2021 , 42 (5) : 2550-2557 . |
| MLA | Li, Xi et al. "3种常用除草剂对细菌抗生素耐药性的影响" . | 环境科学 42 . 5 (2021) : 2550-2557 . |
| APA | Li, Xi , Liao, Han-Peng , Cui, Peng , Bai, Yu-Dan , Liu, Chen , Wen, Chang et al. 3种常用除草剂对细菌抗生素耐药性的影响 . | 环境科学 , 2021 , 42 (5) , 2550-2557 . |
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