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学者姓名：陈栩
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Abstract ：
Enhancing nitrogen-use efficiency is essential for boosting crop yields and advancing sustainable agriculture, particularly in the absence of synthetic fertilizers. Despite the inherent nitrogen-fixation capacity of the staple legume crop soybean (Glycine max) by symbiotic rhizobia, improving nitrogen use has been challenging. Here, a role for the auxin-efflux transporters PIN3a and PIN3b in soybean nitrate acquisition is uncovered. PIN3a/b localizes to the plasma membrane, and high environmental nitrate induces PIN3a degradation and its accumulation at cell junctions. Disrupting PIN3 homologs results in auxin over-accumulation, impairs pavement-cell polarity, and enhances signaling via the transcription factors ARF and STF3/4. These transcription factors separately bind to and activate the NPF2.13 promoter, thereby strengthening nitrate uptake. pin3ab and pin3abd mutants have enhanced nitrate acquisition and resistant to high nitrate on pavement-cell growth. The elevated nitrogen accumulation translates to higher oil contents in pin3ab mutant seeds in an elite cultivar background across multiple years and field locations. The findings shed light on the regulation of nitrate uptake in crop-plant development and demonstrate the unexpected potential of manipulating auxin transporters to enhance soybean nitrogen-use efficiency and agronomic performance.
Keyword ：
auxin auxin leaf development leaf development nitrate nitrate PIN3 PIN3 seed oil seed oil soybean soybean
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| GB/T 7714 | Xu, Huifang , Huang, Shiyu , Wang, Jie et al. Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits [J]. | ADVANCED SCIENCE , 2025 . |
| MLA | Xu, Huifang et al. "Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits" . | ADVANCED SCIENCE (2025) . |
| APA | Xu, Huifang , Huang, Shiyu , Wang, Jie , Wang, Tian , Han, Qingqing , Wu, Kexin et al. Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits . | ADVANCED SCIENCE , 2025 . |
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Symbiotic nodules comprise two classes, indeterminate and determinate, defined by the presence/absence of apical meristem and developmental zonation. Why meristem and zonation are absent from determinate nodules remains unclear. Here, we define cell types in developing soybean nodules, highlighting the undifferentiated infection zones and differentiated nitrogen-fixation zones. Auxin governs infection zone maintenance. GRETCHEN HAGEN 3 (GH3) enzymes deactivate auxin by conjugation and promote cell differentiation. gh3 mutants increased undifferentiated cells and enlarged infection zones. The central symbiosis-transcription factor NIN2a activates GH3.1 to reduce auxin levels and facilitates cell differentiation. High auxin promotes NIN2a protein accumulation and enhances signaling, further deactivating auxin and depleting infection zones. Our findings shed light on the NIN2a-GH3-auxin module that drives soybean nodule cell differentiation. This study challenges our understanding of determinate nodule development and proposes that the regulation of nodule zonation offers valuable insights into broader mechanisms of cell differentiation across plant species.
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| GB/T 7714 | Tu, Tianli , Gao, Zhen , Li, Linfang et al. Soybean symbiotic-nodule zonation and cell differentiation are defined by NIN2 signaling and GH3-dependent auxin homeostasis [J]. | DEVELOPMENTAL CELL , 2024 , 59 (16) . |
| MLA | Tu, Tianli et al. "Soybean symbiotic-nodule zonation and cell differentiation are defined by NIN2 signaling and GH3-dependent auxin homeostasis" . | DEVELOPMENTAL CELL 59 . 16 (2024) . |
| APA | Tu, Tianli , Gao, Zhen , Li, Linfang , Chen, Jiansheng , Ye, Kangzhuo , Xu, Tao et al. Soybean symbiotic-nodule zonation and cell differentiation are defined by NIN2 signaling and GH3-dependent auxin homeostasis . | DEVELOPMENTAL CELL , 2024 , 59 (16) . |
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生长素几乎参与植物生长发育的每一个过程,是最重要的植物激素之一.基于模式植物的研究证实,生长素通过合成、代谢、极性运输和信号途径协同建立生长素的浓度梯度和局部浓度差异,决定了植物器官的发生、极性建立和对环境的适应.伴随基因编辑技术在农业领域的广泛应用,如何将生长素途径的理论成果应用于作物改良,通过优势基因的选择和聚合协调作物的理想株型/根型,是该领域聚焦的关键问题.本文总结了近五年模式植物中解析的生长素领域的最新研究进展,并以我国重要的经济作物大豆的株型和根型改良为目标,全面阐述和预测了生长素在大豆育种中的潜在应用价值.
Keyword ：
大豆 大豆 株型 株型 根形态建成 根形态建成 生长素 生长素
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| GB/T 7714 | 徐慧芳 , 陈栩 . 生长素研究现状及其在大豆育种中的应用 [J]. | 中国科学:生命科学 , 2024 , 54 (02) : 247-259 . |
| MLA | 徐慧芳 et al. "生长素研究现状及其在大豆育种中的应用" . | 中国科学:生命科学 54 . 02 (2024) : 247-259 . |
| APA | 徐慧芳 , 陈栩 . 生长素研究现状及其在大豆育种中的应用 . | 中国科学:生命科学 , 2024 , 54 (02) , 247-259 . |
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While it is well known that plants interpret UV-B as an environmental cue and a potential stressor influencing their growth and development, the specific effects of UV-B-induced oxidative stress on the dynamics of membrane lipids and proteins remain underexplored. Here, we demonstrate that UV-B exposure notably increases the formation of ordered lipid domains on the plasma membrane (PM) and significantly alters the behavior of the Glycine max nodule autoregulation receptor kinase (GmNARK) protein in Arabidopsis leaves. The GmNARK protein was located on the PM and accumulated as small particles in the cytoplasm. We found that UV-B irradiation interrupted the lateral diffusion of GmNARK proteins on the PM. Furthermore, UV-B light decreases the efficiency of surface molecule internalization by clathrin-mediated endocytosis (CME). In brief, UV-B irradiation increased the proportion of the ordered lipid phase and disrupted clathrin-dependent endocytosis; thus, the endocytic trafficking and lateral mobility of GmNARK protein on the plasma membrane are crucial for nodule formation tuning. Our results revealed a novel role of low-intensity UV-B stress in altering the organization of the plasma membrane and the dynamics of membrane-associated proteins.
Keyword ：
endocytosis endocytosis GmNARK GmNARK lipid organization lipid organization UV-B UV-B
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| GB/T 7714 | Liu, Qiulin , Wang, Tianyu , Ke, Meiyu et al. UV-B Radiation Disrupts Membrane Lipid Organization and Suppresses Protein Mobility of GmNARK in Arabidopsis [J]. | PLANTS-BASEL , 2024 , 13 (11) . |
| MLA | Liu, Qiulin et al. "UV-B Radiation Disrupts Membrane Lipid Organization and Suppresses Protein Mobility of GmNARK in Arabidopsis" . | PLANTS-BASEL 13 . 11 (2024) . |
| APA | Liu, Qiulin , Wang, Tianyu , Ke, Meiyu , Qian, Chongzhen , Li, Jiejie , Huang, Xi et al. UV-B Radiation Disrupts Membrane Lipid Organization and Suppresses Protein Mobility of GmNARK in Arabidopsis . | PLANTS-BASEL , 2024 , 13 (11) . |
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Legume nodulation requires light perception by plant shoots and precise long-distance communication between shoot and root. Recent studies have revealed that TGACG-motif binding factors (GmSTFs) integrate light signals to promote root nodulation; however, the regulatory mechanisms underlying nodule formation in changing light conditions remain elusive.Here, we applied genetic engineering, metabolite measurement, and transcriptional analysis to study soybean (Glycine max) nodules.We clarify a fine-tuning mechanism in response to ultraviolet B (UV-B) irradiation and rhizobia infection, involving GmUVR8-dependent UV-B perception and GmSTF3/4-GmMYB12-GmCHS-mediated (iso)flavonoid biosynthesis for soybean nodule formation. GmUVR8 receptor-perceived UV-B signal triggered R2R3-MYB transcription factors GmMYB12-dependent flavonoid biosynthesis separately in shoot and root. In shoot, UV-B-triggered flavonoid biosynthesis relied on GmUVR8a, b, c receptor-dependent activation of GmMYB12L-GmCHS8 (chalcone synthase) module. In root, UV-B signaling distinctly promotes the accumulation of the isoflavones, daidzein, and its derivative coumestrol, via GmMYB12B2-GmCHS9 module, resulting in hypernodulation. The mobile transcription factors, GmSTF3/4, bind to cis-regulatory elements in the GmMYB12L, GmMYB12B2, and GmCHS9 promoters, to coordinate UV-B light perception in shoot and (iso)flavonoid biosynthesis in root.Our findings establish a novel shoot-to-root communication module involved in soybean nodulation and reveal an adaptive strategy employed by soybean roots in response to UV-B light.
Keyword ：
flavonoid flavonoid GmUVR8 GmUVR8 nodule nodule soybean soybean ultraviolet B ultraviolet B
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| GB/T 7714 | Chen, Jiansheng , Xu, Huifang , Liu, Qiulin et al. Shoot-to-root communication via GmUVR8-GmSTF3 photosignaling and flavonoid biosynthesis fine-tunes soybean nodulation under UV-B light [J]. | NEW PHYTOLOGIST , 2023 , 241 (1) : 209-226 . |
| MLA | Chen, Jiansheng et al. "Shoot-to-root communication via GmUVR8-GmSTF3 photosignaling and flavonoid biosynthesis fine-tunes soybean nodulation under UV-B light" . | NEW PHYTOLOGIST 241 . 1 (2023) : 209-226 . |
| APA | Chen, Jiansheng , Xu, Huifang , Liu, Qiulin , Ke, Meiyu , Zhang, Zhongqin , Wang, Xu et al. Shoot-to-root communication via GmUVR8-GmSTF3 photosignaling and flavonoid biosynthesis fine-tunes soybean nodulation under UV-B light . | NEW PHYTOLOGIST , 2023 , 241 (1) , 209-226 . |
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Breeding crop varieties with high yield and ideal plant architecture is a desirable goal of agricultural science. The success of "Green Revolution" in cereal crops provides opportunities to incorporate phytohormones in crop breeding. Auxin is a critical phytohormone to determine nearly all the aspects of plant development. Despite the current knowledge regarding auxin biosynthesis, auxin transport and auxin signaling have been well characterized in model Arabidopsis (Arabidopsis thaliana) plants, how auxin regulates crop architecture is far from being understood, and the introduction of auxin biology in crop breeding stays in the theoretical stage. Here, we give an overview on molecular mechanisms of auxin biology in Arabidopsis, and mainly summarize auxin contributions for crop plant development. Furthermore, we propose potential opportunities to integrate auxin biology in soybean (Glycine max) breeding.
Keyword ：
Auxin Auxin Breeding Breeding Crop Crop Soybean Soybean
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| GB/T 7714 | Li, Linfang , Chen, Xu . Auxin regulation on crop: from mechanisms to opportunities in soybean breeding [J]. | MOLECULAR BREEDING , 2023 , 43 (3) . |
| MLA | Li, Linfang et al. "Auxin regulation on crop: from mechanisms to opportunities in soybean breeding" . | MOLECULAR BREEDING 43 . 3 (2023) . |
| APA | Li, Linfang , Chen, Xu . Auxin regulation on crop: from mechanisms to opportunities in soybean breeding . | MOLECULAR BREEDING , 2023 , 43 (3) . |
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生长素作为重要的植物激素之一。生长素的转运参与了植物各组织器官发育调控。在拟南芥中,生长素转运调控主要由PIN家族蛋白所介导,其中拟南芥AtPIN2主要通过介导生长素向基运输调控根的向重力性。大豆中PIN2家族蛋白及其功能研究尚未有报道。本研究通过构建系统进化树和蛋白质结构域分析发现,大豆GmPIN2a和GmPIN2b为AtPIN2的同源基因。组织表达分析研究发现, GmPIN2a和GmPIN2b在根、根瘤原基和根瘤等组织中高表达。GmPIN2a和GmPIN2b在根部主要表达在根尖表皮和外部皮层细胞,在根瘤中GmPIN2a、GmPIN2b均定位于根瘤基部维管束区,此外,GmPIN2a定位在根瘤顶部表皮及外皮层。利用基因编辑技术CRISPR/Cas9(Clustered RegularlyInterspersedShortPalindromicRepeats/CRISPRassociated9)同时敲除GmPIN2a和GmPIN2b后发现,Gmpin2ab突变体根具有明显的重力缺失表型。与野生型相比, Gmpin2ab和35S::GmPIN2b的根面积和侧根长度显著降低,Gmpin2ab侧根夹角显著上升,而35S::GmPIN2b侧根夹角不变。综上所述,GmPIN2a和GmPIN2b通过介导生长素向基运输对大豆根型调控具有重要作用。本研究为深入探究大豆PIN蛋白介导生长素极性运输在根形态建成的作用机制奠定了一定研究基础。
Keyword ：
PIN蛋白 PIN蛋白 大豆 大豆 根 根 生长素 生长素
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| GB/T 7714 | 梁政 , 柯美玉 , 陈志威 et al. 大豆GmPIN2家族基因调控根系发育功能初探 [J]. | 作物学报 , 2023 , 49 (01) : 24-35 . |
| MLA | 梁政 et al. "大豆GmPIN2家族基因调控根系发育功能初探" . | 作物学报 49 . 01 (2023) : 24-35 . |
| APA | 梁政 , 柯美玉 , 陈志威 , 陈栩 , 高震 . 大豆GmPIN2家族基因调控根系发育功能初探 . | 作物学报 , 2023 , 49 (01) , 24-35 . |
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Multi-copper oxidases SKU5 and SKS1 modulate reactive oxygen species homeostasis in root apoplast to regulate cell wall formation. The primary cell wall is a fundamental plant constituent that is flexible but sufficiently rigid to support the plant cell shape. Although many studies have demonstrated that reactive oxygen species (ROS) serve as important signaling messengers to modify the cell wall structure and affect cellular growth, the regulatory mechanism underlying the spatial-temporal regulation of ROS activity for cell wall maintenance remains largely unclear. Here, we demonstrate the role of the Arabidopsis (Arabidopsis thaliana) multicopper oxidase-like protein skewed 5 (SKU5) and its homolog SKU5-similar 1 (SKS1) in root cell wall formation through modulating ROS homeostasis. Loss of SKU5 and SKS1 function resulted in aberrant division planes, protruding cell walls, ectopic deposition of iron, and reduced nicotinamide adeninedinucleotide phosphate (NADPH) oxidase-dependent ROS overproduction in the root epidermis-cortex and cortex-endodermis junctions. A decrease in ROS level or inhibition of NADPH oxidase activity rescued the cell wall defects of sku5 sks1 double mutants. SKU5 and SKS1 proteins were activated by iron treatment, and iron over-accumulated in the walls between the root epidermis and cortex cell layers of sku5 sks1. The glycosylphosphatidylinositol-anchored motif was crucial for membrane association and functionality of SKU5 and SKS1. Overall, our results identified SKU5 and SKS1 as regulators of ROS at the cell surface for regulation of cell wall structure and root cell growth.
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| GB/T 7714 | Chen, Chaofan , Zhang, Yi , Cai, Jianfa et al. Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots [J]. | PLANT PHYSIOLOGY , 2023 , 192 (3) : 2243-2260 . |
| MLA | Chen, Chaofan et al. "Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots" . | PLANT PHYSIOLOGY 192 . 3 (2023) : 2243-2260 . |
| APA | Chen, Chaofan , Zhang, Yi , Cai, Jianfa , Qiu, Yuting , Li, Lihong , Gao, Chengxu et al. Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots . | PLANT PHYSIOLOGY , 2023 , 192 (3) , 2243-2260 . |
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生物固氮是豆科植物特有的低能耗、无污染的氮素供应形式,充分挖掘豆科植物的生物固氮机制,提高结瘤和固氮效率,对于降低农业投入,维持绿色环保降低污染,促进农业可持续发展具有重要的意义。根瘤的形成是共生固氮体系建立的前提,它的发生、发育及成熟的过程涉及到植物根系和微生物建立多种多样的共生关系,其中植物激素整合了外界环境信号和根瘤发育的内源信号,对根瘤的发生和形成发挥了关键的作用。合理的利用激素有望实现根瘤数目和固氮的精准控制。本课题的研究表明,大豆中的生长素极性运输GmPIN1蛋白负责极性运输生长素从根至根瘤原基发生处,控制根瘤起始阶段的生长素浓度梯度建立,参与大豆根瘤的发生。把控根瘤菌附着的黄酮类化合物促进GmPIN1的侧向化分布,控制根瘤原基细胞分裂的细胞分裂素刺激GmPIN1的亚细胞极性发生侧向化。此外,大豆特异进化的GmPIN9d蛋白与GmPIN1协调控制根瘤发育后期生长素在根-根瘤维管束交界区域的极性流动,参与大豆根瘤后期的膨大。因而,我们获得了GmPIN1和GmPIN9d分别控制根瘤早期发生和后期发育的生长素调控模型(Gao et al., Plant Cell)。基于以上生长素调控大豆根瘤发生的研究基础之上,我们进一步聚焦于如何在农业种植过程中高效利用"生长素控瘤"的机制。我们发现大豆地上部分在响应GmUVR8受体介导的紫外光信号后,地下部分的根瘤数量显著增加1.5倍以上。此紫外光刺激大豆超结瘤的现象依赖于GmUVR8受体精准控制叶片中的的黄酮合成以及叶-根的黄酮运输。紫外光-黄酮信号传递到根部后,由生长素运输途径响应并精细调节根瘤的发生和数量(Ke et al., submitted)。根据以上两方面的发现,我们将地上部分的光信号和地下部分的生长素运输途径有效的结合,并为结瘤固氮高效的大豆种植和分子育种提供明确的理论指导。
Keyword ：
GmPIN GmPIN 大豆 大豆 根瘤 根瘤 生长素 生长素 紫外光 紫外光
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| GB/T 7714 | 高震 , 柯美玉 , 徐慧芳 et al. 生长素调控大豆根瘤发育的机制及应用 [C] //首届植物科学前沿学术大会摘要集（二） . 2022 . |
| MLA | 高震 et al. "生长素调控大豆根瘤发育的机制及应用" 首届植物科学前沿学术大会摘要集（二） . (2022) . |
| APA | 高震 , 柯美玉 , 徐慧芳 , 陈栩 . 生长素调控大豆根瘤发育的机制及应用 首届植物科学前沿学术大会摘要集（二） . (2022) . |
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Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role of FvPME38 in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level of FvPME38, thereby accelerating fruit softening. During fruit development, FvMYB79 co-expressed with FvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of 'Ruegen' which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level of FvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing of FvMYB79 showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement of FvMYB79 in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation of FvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.
Keyword ：
fruit softening fruit softening FvMYB79 FvMYB79 FvPME38 FvPME38 strawberry strawberry
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| GB/T 7714 | Cai, Jianfa , Mo, Xuelian , Wen, Chenjin et al. FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38 [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (1) . |
| MLA | Cai, Jianfa et al. "FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 . 1 (2022) . |
| APA | Cai, Jianfa , Mo, Xuelian , Wen, Chenjin , Gao, Zhen , Chen, Xu , Xue, Cheng . FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38 . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (1) . |
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