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学者姓名：朱强
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Abstract ：
Ma bamboo (Dendrocalamus latiflorus Munro) is a key giant bamboo in South Asia, highly sensitive to abiotic stresses. Dehydration-Responsive Element-Binding (DREB) transcription factors (TFs) are an important gene family involved in plant growth, metabolic regulation, and environmental responses. Although the roles of DREB TFs in plant growth, metabolic regulation, and environmental responses are well studied, little is known in Ma bamboo. To address this gap, we systematically identified and functuonally predicted DREB genes in Ma bamboo, with emphasis on their potential involvement in stress response mechanisms. Through genome-wide analysis, 42 DlDREBs distributed across 6 subfamilies were identified. Integrative analyses encompassin cis-element, chromosomal localization, phylogenetic relationships, protein-protein interaction (PPI) network, and gene ontology (GO) annotations revealed their putative roles in developmental processes, metabolic modulation, and stress adaptation. RT-qPCR profiling demonstrated that eight DlDREBs exhibit distinct and stress-specific expression patterns under cold, salt, and drought treatments, underscoring their pivotal contributions to abiotic stress resilience. Three candidate genes (DREB9-A, DREB10-B, and DREB12-C) were prioritized for functional validation. Subsequent cloning, sequence characterization, subcellular localization analysis, and DNA binding assays confirmed their regulatory potential. Notably, DREB10-B, and DREB12-C were found to directly bind the promoter of GA2ox7, a key gene implicated in abiotic stress signaling, suggesting their mechanistic role in stress-responsive pathways. Furthermore, transient transformation assays in tobacco and yeast transformation experiments demonstrated that overexpression of DREB10-B and DREB12-C may enhance plant stress tolerance. In summary, this study provides a theoretical basis for clarifying the molecular mechanism of the abiotic stress responses of the DREB gene family in Ma bamboo.
Keyword ：
Abiotic stress response Abiotic stress response DREB transcription factors DREB transcription factors Evolution and collinearity analysis Evolution and collinearity analysis Gene ontology Gene ontology Ma bamboo Ma bamboo
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| GB/T 7714 | Liang, Jianxiang , Zhou, Linying , Hu, Xin et al. Functional and expression profiling of DREB genes in Ma Bamboo (Dendrocalamus latiflorus Munro) reveals their role in abiotic stress adaptation [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 228 . |
| MLA | Liang, Jianxiang et al. "Functional and expression profiling of DREB genes in Ma Bamboo (Dendrocalamus latiflorus Munro) reveals their role in abiotic stress adaptation" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 228 (2025) . |
| APA | Liang, Jianxiang , Zhou, Linying , Hu, Xin , Lu, Jiang , Wang, Wenjia , Zhu, Qiang . Functional and expression profiling of DREB genes in Ma Bamboo (Dendrocalamus latiflorus Munro) reveals their role in abiotic stress adaptation . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 228 . |
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In a recent study, Hou et al. devel-oped a high-resolution, haplotype-based pangenome for moso bamboo (Phyllostachys edulis), re-vealing significant genetic diversity and over 1000 climate-associated variants. Their findings highlight adaptive mechanisms for the eco-logical resilience of bamboo, pro-viding crucial insights for climate-resilient breeding and conservation to ensure the long-term ecological and economic benefits of moso bamboo amid climate change
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| GB/T 7714 | Wang, Nannan , Wang, Wenjia , Zhu, Qiang . Unlocking the genetic blueprint of bamboo for climate adaption [J]. | TRENDS IN PLANT SCIENCE , 2025 , 30 (7) : 693-695 . |
| MLA | Wang, Nannan et al. "Unlocking the genetic blueprint of bamboo for climate adaption" . | TRENDS IN PLANT SCIENCE 30 . 7 (2025) : 693-695 . |
| APA | Wang, Nannan , Wang, Wenjia , Zhu, Qiang . Unlocking the genetic blueprint of bamboo for climate adaption . | TRENDS IN PLANT SCIENCE , 2025 , 30 (7) , 693-695 . |
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De novo shoot organogenesis (DNSO) enables plants to regenerate shoots from various explants, offering valuable opportunities for research and plant biotechnology applications. While significant progress has been made in understanding regeneration in angiosperms, the regulatory mechanisms in gymnosperms, particularly Chinese fir (Cunninghamia lanceolata), remain poorly understood, despite its importance as a key timber species in China. This study successfully established an efficient DNSO protocol for Chinese fir, identifying six distinct stages in the process through cellular-level analysis. Time-course transcriptome and proteomics analyses revealed dynamic changes in mRNA and protein levels during regeneration. Notably, proteins showed more significant alterations across a broad range of biological processes, often independent of corresponding mRNA changes. Key pathways associated with ethylene metabolism and abiotic stress responses were enriched, highlighting their critical roles in regeneration. Further experiments confirmed that moderate osmotic stress treatments (150 mm mannitol) and ethylene treatment (100 mu m ACC and 5 mu m AgNO3) substantially enhanced DNSO efficiency. In summary, this study uncovers the molecular mechanisms underlying Chinese fir DNSO, providing valuable insights into improving plant regeneration efficiency in this economically important species. These findings contribute to advancements in plant biotechnology and sustainable forestry practices.
Keyword ：
abiotic stress abiotic stress Chinese fir Chinese fir De novo shoot organogenesis De novo shoot organogenesis proteome proteome transcriptome transcriptome
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| GB/T 7714 | Ding, Wensha , Ye, Shanwen , Wu, Duoke et al. Time-course transcriptome and proteomic dynamics during the de novo shoot organogenesis in Chinese fir (Cunninghamia lanceolata) [J]. | PLANT JOURNAL , 2025 , 123 (3) . |
| MLA | Ding, Wensha et al. "Time-course transcriptome and proteomic dynamics during the de novo shoot organogenesis in Chinese fir (Cunninghamia lanceolata)" . | PLANT JOURNAL 123 . 3 (2025) . |
| APA | Ding, Wensha , Ye, Shanwen , Wu, Duoke , Wang, Wanping , Xu, Junjie , Wang, Wenjia et al. Time-course transcriptome and proteomic dynamics during the de novo shoot organogenesis in Chinese fir (Cunninghamia lanceolata) . | PLANT JOURNAL , 2025 , 123 (3) . |
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Bamboo is a fast-growing and ecologically significant plant with immense economic value due to its applications in construction, textiles, and bioenergy. However, research on bamboo has been hindered by its long vegetative period, unpredictable flowering cycles, and challenges in genetic transformation. Recent developments in advanced sequencing and genetic engineering technologies have provided new insights into bamboo's evolutionary history, developmental biology, and stress resilience, paving the way for improved conservation and sustainable utilization. This review synthesizes the latest findings on bamboo's genomics, biotechnology, and the molecular mechanisms governing its growth, development, and stress response. Key genes and regulatory pathways controlling its rapid growth, internode elongation, rhizome development, culm lignification, flowering, and abiotic stress responses have been identified through multi-omics and functional studies. Complex interactions among transcription factors, epigenetic regulators, and functionally important genes shape bamboo's unique growth characteristics. Moreover, progress in genetic engineering techniques, including clustered regularly interspaced short palindromic repeats-based genome editing, has opened new avenues for targeted genetic improvements. However, technical challenges, particularly the complexity of polyploid bamboo genomes and inefficient regeneration systems, remain significant barriers to functional studies and large-scale breeding efforts. By integrating recent genomic discoveries with advancements in biotechnology, this review proposes potential strategies to overcome existing technological limitations and to accelerate the development of improved bamboo varieties. Continued efforts in multi-omics research, gene-editing applications, and sustainable cultivation practices will be essential for harnessing bamboo as a resilient and renewable resource for the future. The review presented here not only deepens our understanding of bamboo's genetic architecture but also provides a foundation for future research aimed at optimizing its ecological and industrial potential.
Keyword ：
abiotic stress response abiotic stress response bamboo genomics bamboo genomics genetic engineering genetic engineering growth and development growth and development
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| GB/T 7714 | Wang, Wenjia , Wu, Qiyao , Wang, Nannan et al. Advances in bamboo genomics: Growth and development, stress tolerance, and genetic engineering [J]. | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2025 , 67 (7) : 1725-1755 . |
| MLA | Wang, Wenjia et al. "Advances in bamboo genomics: Growth and development, stress tolerance, and genetic engineering" . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY 67 . 7 (2025) : 1725-1755 . |
| APA | Wang, Wenjia , Wu, Qiyao , Wang, Nannan , Ye, Shanwen , Wang, Yujun , Zhang, Jiang et al. Advances in bamboo genomics: Growth and development, stress tolerance, and genetic engineering . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2025 , 67 (7) , 1725-1755 . |
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Lignin biosynthesis is vital for plant growth and development. In model plants, 4-coumarate: coenzyme A ligase (4CL) and caffeic acid O-methyltransferase (COMT) are key regulators of lignin content and composition. However, their functions in gymnosperms, especially conifers, remain largely unexplored. Here, we cloned and functionally characterized the orthologs of these genes in Chinese fir (Cunninghamia lanceolata), the major timber species in China. Enzymatic assays confirmed that Cl4CL catalyzes the conversion of caffeic and p-coumaric acids into CoA esters, while ClCOMT methylates caffeic acid to produce ferulic acid, both critical intermediates in lignin biosynthesis. Ectopic expression of Cl4CL and ClCOMT in poplar altered lignin content and caused dwarfism. Importantly, we successfully generated transgenic Chinese fir lines overexpressing Cl4CL and ClCOMT for the first time. These transgenic lines exhibited reduced height, smaller xylem and pith areas, and increased lignin content. Our findings reveal both conserved and species-specific functions of Cl4CL and ClCOMT in Chinese fir and provide valuable insights for lignin modification. This work offers a foundation for genetic improvement strategies aimed at optimizing lignin traits and supports the sustainable utilization of forest resources.
Keyword ：
4CL gene 4CL gene Chinese fir Chinese fir COMT gene COMT gene Genetic transformation Genetic transformation Lignin Lignin Protease activity Protease activity
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| GB/T 7714 | Cai, Changyang , Ye, Shanwen , Liu, Jiayi et al. Functional insights into 4-coumarate-CoA ligase and caffeic acid O-methyltransferase genes involved in lignin biosynthesis in Chinese fir (Cunninghamia lanceolata) [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 322 . |
| MLA | Cai, Changyang et al. "Functional insights into 4-coumarate-CoA ligase and caffeic acid O-methyltransferase genes involved in lignin biosynthesis in Chinese fir (Cunninghamia lanceolata)" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 322 (2025) . |
| APA | Cai, Changyang , Ye, Shanwen , Liu, Jiayi , Wang, Nannan , Tu, Min , Ding, Wensha et al. Functional insights into 4-coumarate-CoA ligase and caffeic acid O-methyltransferase genes involved in lignin biosynthesis in Chinese fir (Cunninghamia lanceolata) . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 322 . |
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Lignin contributes to plant mechanical properties during bending loads. Meanwhile, phytohormone auxin controls various plant biological processes. However, the mechanism of auxin's role in bending-induced lignin biosynthesis was unclear, especially in bamboo, celebrated for its excellent deformation stability.Here, we reported that auxin response factors (ARF) 3 and ARF6 from Moso bamboo (Phyllostachys edulis (Carriere) J. Houz) directly regulate lignin biosynthesis pathway genes, and affect lignin biosynthesis in bamboo.Auxin and lignin exhibited asymmetric distribution patterns, and auxin promoted lignin biosynthesis in response to bending loads in bamboo. Employing transcriptomic and weighted gene co-expression network analysis approach, we discovered that expression patterns of ARF3 and ARF6 strongly correlated with lignin biosynthesis genes. ARF3 and ARF6 directly bind to the promoter regions of 4-coumarate: coenzyme A ligase (4CL3, 4CL7, and 4CL9) or caffeoyl-CoA O-methyltransferase (CCoAOMT2) genes, pivotal to lignin biosynthesis, and activate their expressions. Notably, the efficacy of this binding hinges on auxin levels. Alternation of the expressions of ARF3 and ARF6 substantially altered lignin accumulation in transgenic bamboo.Collectively, our study shed light on bamboo lignification genetics. Auxin signaling could directly modulate lignin biosynthesis genes to impact plant lignin content.
Keyword ：
auxin auxin auxin response factors auxin response factors bamboo bamboo bending bending lignin lignin
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| GB/T 7714 | Wang, Wenjia , Li, Yigang , Cai, Changyang et al. Auxin response factors fine-tune lignin biosynthesis in response to mechanical bending in bamboo [J]. | NEW PHYTOLOGIST , 2023 , 241 (3) : 1161-1176 . |
| MLA | Wang, Wenjia et al. "Auxin response factors fine-tune lignin biosynthesis in response to mechanical bending in bamboo" . | NEW PHYTOLOGIST 241 . 3 (2023) : 1161-1176 . |
| APA | Wang, Wenjia , Li, Yigang , Cai, Changyang , Zhu, Qiang . Auxin response factors fine-tune lignin biosynthesis in response to mechanical bending in bamboo . | NEW PHYTOLOGIST , 2023 , 241 (3) , 1161-1176 . |
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低温作为一种主要的非生物胁迫，会使植物在生长过程中受到损伤，严重阻碍植物的正常生长和分布，并降低经济作物的产量，最终对自然界的稳定和社会生产活动产生不利影响。开展植物响应低温机制的相关研究具有重要的实践应用价值。本文综述在低温胁迫下植物冷驯化现象的发生及相关生理生化指标(如可溶性糖、脯氨酸、活性氧等)的变化，并着重介绍植物响应冷胁迫信号的网络调控机制，包括CBF依赖型信号转导途径和非CBF依赖型信号转导途径。具体描述CBF依赖型信号转导途径中最重要的ICE1-CBF-COR信号级联通路，CBF转录调控的正、负调控以及调控途径中的转录后调控、翻译后调控等方面内容。归纳目前基于基因工程技术提高水稻、玉米、小麦、番茄等农业作物抗寒性的研究进展，旨在为进一步提高植物抗寒性和培育耐寒植物新品种提供一定的参考。
Keyword ：
CBF基因 CBF基因 ICE1基因 ICE1基因 低温胁迫 低温胁迫 植物 植物 转基因 转基因
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| GB/T 7714 | 吴宇欣 , 蔡昌杨 , 唐诗蓓 et al. 植物响应低温的生长发育及分子机制研究进展 [J]. | 江苏农业科学 , 2023 , 51 (19) : 1-9 . |
| MLA | 吴宇欣 et al. "植物响应低温的生长发育及分子机制研究进展" . | 江苏农业科学 51 . 19 (2023) : 1-9 . |
| APA | 吴宇欣 , 蔡昌杨 , 唐诗蓓 , 谢裕红 , 王晓艳 , 朱强 . 植物响应低温的生长发育及分子机制研究进展 . | 江苏农业科学 , 2023 , 51 (19) , 1-9 . |
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寒冷、干旱和高盐等非生物胁迫作为常见的不利环境条件，严重影响全球植物生长和生产力。干旱应答元件结合蛋白（dehydration responsive element binding protein, DREB）是植物重要转录因子之一，其家族成员均含有一个57-70个氨基酸残基的保守AP2结构域。DREB通过与胁迫诱导基因启动子区中的脱水反应元件/C-重复（dehydration responsive element/C-repeat, DRE/CRT）顺式作用元件相互作用，调节下游各种应激基因的表达，赋予植物应激耐受性。本文从DREB家族结构特点和分类出发，结合最新研究进展，阐述其在非生物胁迫过程中的作用机制，旨在更加深入地了解DERB类转录因子在非生物胁迫响应过程中的分子调控网络，以期为未来利用基因工程手段提高植物抗逆性方面提供参考。
Keyword ：
DREB DREB 转录因子 转录因子 非生物逆境胁迫 非生物逆境胁迫
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| GB/T 7714 | 韩芳英 , 胡昕 , 王楠楠 et al. DREBs响应植物非生物逆境胁迫研究进展 [J]. | 生物技术通报 , 2023 , 39 (11) : 86-98 . |
| MLA | 韩芳英 et al. "DREBs响应植物非生物逆境胁迫研究进展" . | 生物技术通报 39 . 11 (2023) : 86-98 . |
| APA | 韩芳英 , 胡昕 , 王楠楠 , 谢裕红 , 王晓艳 , 朱强 . DREBs响应植物非生物逆境胁迫研究进展 . | 生物技术通报 , 2023 , 39 (11) , 86-98 . |
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Bamboo is an important non-timber forest product and is well-known for its reluctance to regenerate. Recently we have established a de novo shoot organogenesis (DNSO) protocol in Ma bamboo (Dendrocalamus latiflorus) and revealed the transcriptomic dynamics during Ma bamboo regeneration, which suggested the potential roles of Ma bamboo microRNAs (DlamiRNAs) in this process. However, how DlamiRNAs regulate bamboo DNSO is poorly understood. Here we performed integrated analysis with sRNAome, degradome, and transcriptome sequencing by using samples covering the four stages of the bamboo DNSO process. A total of 727 DlamiRNAs showed differential expression during the bamboo DNSO process, and the core DlamiRNA-DlamRNA- mediated regulatory networks for bamboo DNSO were constructed. Based on the results, DlamiR156 was selected for further functional characterization of its potential roles in bamboo DNSO. Transgenic bamboos with increased DlamiR156 levels exhibited an enhancement in their regeneration efficiency. Conversely, when DlamiR156 levels were downregulated, the regeneration efficiencies of transgenic bamboos decreased. Our findings show that the DlamiRNA-mediated regulatory pathways are significant in the process of bamboo regeneration and will contribute to our understanding of the molecular mechanisms governing plant organogenesis in a more comprehensive manner.
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| GB/T 7714 | Wang, Nannan , Wang, Wenjia , Cheng, Yang et al. Uncovering the miRNA-mediated regulatory network involved in Ma bamboo (Dendrocalamus latiflorus) de novo shoot organogenesis [J]. | HORTICULTURE RESEARCH , 2023 , 10 (12) . |
| MLA | Wang, Nannan et al. "Uncovering the miRNA-mediated regulatory network involved in Ma bamboo (Dendrocalamus latiflorus) de novo shoot organogenesis" . | HORTICULTURE RESEARCH 10 . 12 (2023) . |
| APA | Wang, Nannan , Wang, Wenjia , Cheng, Yang , Cai, Changyang , Zhu, Qiang . Uncovering the miRNA-mediated regulatory network involved in Ma bamboo (Dendrocalamus latiflorus) de novo shoot organogenesis . | HORTICULTURE RESEARCH , 2023 , 10 (12) . |
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DELLA蛋白是GAs (赤霉素)信号转导途径中的关键因子，但在麻竹中相关的研究仍是空白。为了研究麻竹中DlmDELLA基因的功能，实验通过同源克隆的方法，从麻竹DNA和cDNA中克隆出一条DlmDELLA基因，基因全长c DNA序列为1 863 bp，无内含子，编码620个氨基酸，分子量为65.57 kD，等电点为5.20。蛋白聚类分析，发现麻竹DlmDELLA编码的蛋白与其他植物DELLA蛋白一样具有完整的功能结构域，而且麻竹DELLA蛋白与玉米、水稻、毛竹等禾本科植物的亲缘关系较近。利用STRING数据库对其互作蛋白进行分析发现其与GIDs、PIF3、SLY1等蛋白可能有相互作用。RT-qRCR分析麻竹DlmDELLA的时空表达，发现其在叶、胚芽组织中表达量较高；在外源GA3处理下，基因表达呈现上升趋势，在24 h表达量达到最高；外源PAC处理下，基因表达在3 h上升到最高后下降。原生质体中基因的亚细胞定位，发现DlmDELLA蛋白定位在细胞核中。本研究为进一步研究麻竹中关于GAs信号通路中DELLA蛋白功能及其分子调控机制提供一定的理论依据和参考价值。
Keyword ：
DELLA蛋白 DELLA蛋白 亚细胞定位 亚细胞定位 基因时空表达模式 基因时空表达模式 麻竹(Dendrocalamus latiflorus Munro) 麻竹(Dendrocalamus latiflorus Munro)
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| GB/T 7714 | 陈刚 , 王楠楠 , 白玮元 et al. 麻竹DlmDELLA基因克隆与分析 [J]. | 分子植物育种 , 2023 , 21 (06) : 1874-1883 . |
| MLA | 陈刚 et al. "麻竹DlmDELLA基因克隆与分析" . | 分子植物育种 21 . 06 (2023) : 1874-1883 . |
| APA | 陈刚 , 王楠楠 , 白玮元 , 韩芳英 , 朱强 . 麻竹DlmDELLA基因克隆与分析 . | 分子植物育种 , 2023 , 21 (06) , 1874-1883 . |
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