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学者姓名：王琴
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Paralogous transcription factors (TFs) frequently recognize highly similar DNA motifs. Homodimerization can help distinguish them according to their different dimeric configurations. Here, by studying R2R3-MYB TFs, we show that homodimerization can also directly change the recognized DNA motifs to distinguish between similar TFs. By high-throughput SELEX, we profiled the specificity landscape for 40 R2R3-MYBs of subfamily VIII and curated 833 motif models. The dimeric models show that homodimeric binding has evoked specificity changes for AtMYBs. Focusing on AtMYB2 as an example, we show that homodimerization has modified its specificity and allowed it to recognize additional cis-regulatory sequences that are different from the closely related CCWAA-box AtMYBs and are unique among all AtMYBs. Genomic sites described by the modified dimeric specificities of AtMYB2 are conserved in evolution and involved in AtMYB2-specific transcriptional activation. Collectively, this study provides rich data on sequence preferences of VIII R2R3-MYBs and suggests an alternative mechanism that guides closely related TFs to respective cis-regulatory sites.
Keyword ：
cis-elements cis-elements DNA binding specificity DNA binding specificity homodimerization homodimerization MYB family transcription factors MYB family transcription factors paralogs paralogs regulatory noncoding genome regulatory noncoding genome transcriptional regulation transcriptional regulation
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| GB/T 7714 | Li, Tian , Chen, Hao , Ma, Nana et al. Specificity landscapes of 40 R2R3-MYBs reveal how paralogs target different cis-elements by homodimeric binding [J]. | IMETA , 2025 , 4 (2) . |
| MLA | Li, Tian et al. "Specificity landscapes of 40 R2R3-MYBs reveal how paralogs target different cis-elements by homodimeric binding" . | IMETA 4 . 2 (2025) . |
| APA | Li, Tian , Chen, Hao , Ma, Nana , Jiang, Dingkun , Wu, Jiacheng , Zhang, Xinfeng et al. Specificity landscapes of 40 R2R3-MYBs reveal how paralogs target different cis-elements by homodimeric binding . | IMETA , 2025 , 4 (2) . |
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【目的】研究毛竹miR156(PhemiR156)及其靶基因SPL(PheSPL)在调控植物年龄发育中的作用，为毛竹开花机制研究及遗传改良提供依据。【方法】通过同源序列比对筛选PhemiR156和PheSPL基因并分析其序列、结构及理化性质；利用毛竹转录组数据研究基因表达模式；构建拟南芥PheMIR156和PheSPL异源转基因株系，并分析其年龄发育表型。【结果】在毛竹中鉴定到14个PhemiR156前体基因和36个PheSPL同源基因，其中，17个PheSPL基因有miR156结合位点，32个基因编码含SBP结构域的蛋白。PhemiR156表达量随着毛竹年龄增长而降低，而PheSPL在生殖发育阶段的表达量较高；过表达PheMIR156H显著抑制了拟南芥的年龄发育，而过表达PheSPL19A则导致拟南芥提前进入成年期。【结论】毛竹中的PheMIR156H和靶基因PheSPL19A分别抑制和促进植物年龄发育。
Keyword ：
miR156-SPL分子模块 miR156-SPL分子模块 年龄发育 年龄发育 异源表达 异源表达 拟南芥 拟南芥 毛竹 毛竹
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| GB/T 7714 | 张晓彦 , 陈自银 , 谢开旺 et al. 毛竹miR156靶向SPL19A调节植物年龄发育 [J]. | 福建农林大学学报(自然科学版) , 2025 , 54 (04) : 485-494 . |
| MLA | 张晓彦 et al. "毛竹miR156靶向SPL19A调节植物年龄发育" . | 福建农林大学学报(自然科学版) 54 . 04 (2025) : 485-494 . |
| APA | 张晓彦 , 陈自银 , 谢开旺 , 雷春华 , 骆文浩 , 王琴 et al. 毛竹miR156靶向SPL19A调节植物年龄发育 . | 福建农林大学学报(自然科学版) , 2025 , 54 (04) , 485-494 . |
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High-temperature stress, also referred to as heat stress, often has detrimental effects on plant growth and development. Phytochromes have been implicated in the regulation of plant heat-stress responses, but the role of blue-light receptors, such as cryptochromes, in plant blue-light-dependent heat-stress responses remains unclear. We found that cryptochrome 1 (CRY1) negatively regulates heat-stress tolerance (thermotolerance) in Arabidopsis. Heat stress represses CRY1 phosphorylation. Unphosphorylated CRY1 exhibits decreased activity in suppressing the interaction of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) with ELONGATED HYPOCOTYL 5 (HY5), leading to excessive degradation of HY5 under heat stress in blue light. This reduction in HY5 protein levels subsequently relieves its repression of the transcription of HY5 target genes, especially the heat-shock transcription factors. Our study thus reveals a novel mechanism by which CRY1-mediated blue-light signaling suppresses plant thermotolerance and highlights the dual function of the CRY1-COP1-HY5 module in both light-and heat-stress signaling, providing insights into how plants integrate heat stress and light signals to optimize their survival under heat stress.
Keyword ：
blue light blue light CRY1 CRY1 heat stress heat stress HSF HSF HY5 HY5 thermotolerance thermotolerance
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| GB/T 7714 | Liu, Siyuan , Wang, Qiongli , Zhong, Ming et al. The CRY1-COP1-HY5 axis mediates blue-light regulation of Arabidopsis thermotolerance [J]. | PLANT COMMUNICATIONS , 2025 , 6 (4) . |
| MLA | Liu, Siyuan et al. "The CRY1-COP1-HY5 axis mediates blue-light regulation of Arabidopsis thermotolerance" . | PLANT COMMUNICATIONS 6 . 4 (2025) . |
| APA | Liu, Siyuan , Wang, Qiongli , Zhong, Ming , Lin, Guifang , Ye, Meiling , Wang, Youren et al. The CRY1-COP1-HY5 axis mediates blue-light regulation of Arabidopsis thermotolerance . | PLANT COMMUNICATIONS , 2025 , 6 (4) . |
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A distinct epigenetic feature of plants is the DNA methylation in non-CG contexts. Although the physiological roles of non-CG methylation have been elucidated, its direct impact on transcription factor (TF)-DNA interactions remains largely unexplored. Focusing on WRKY-family TFs, here we investigated how non-CG methylation influences their DNA binding specificity and genome-wide cis-regulatory elements (CREs). By generating 461 SELEX and DAP-seq libraries for 54 AtWRKYs, we show that DNA methylation alters both monomeric and dimeric binding specificities of WRKYs, leading to an overall increase in specificity divergence among family members. We curated 201 WRKY motifs and clustered them into 11 classes, 5 of which represent previously unreported specificities. Notably, the known WRKY cis-element PRE4 was found to be recognized only when methylated. The comprehensive dataset of accurate WRKY motifs also enabled the identification of the amino acid discriminants of W-box and WT-box. Expanding on prior knowledge, we demonstrate that methylation not only decreases but can also increase the affinity of WRKYs. This bidirectional effect has globally reshaped the genomic binding landscape of WRKYs upon methylation. Finally, we constructed the WRKY Regulatory Code Database (https://transysbio.cn/WRKYRCDB.php) to facilitate data access.
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| GB/T 7714 | Ma, Nana , Jiang, Dingkun , Li, Tian et al. The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation [J]. | NUCLEIC ACIDS RESEARCH , 2025 , 53 (21) . |
| MLA | Ma, Nana et al. "The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation" . | NUCLEIC ACIDS RESEARCH 53 . 21 (2025) . |
| APA | Ma, Nana , Jiang, Dingkun , Li, Tian , Luo, Lin , Chen, Hao , Zhang, Xinfeng et al. The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation . | NUCLEIC ACIDS RESEARCH , 2025 , 53 (21) . |
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14-3-3蛋白是一类进化上高度保守的磷酸化识别蛋白，以同源或异源二聚体的形式发挥功能。植物14-3-3蛋白不但参与调控植物开花、叶绿体发育和移动、气孔开放、种子发育、细胞伸长和分裂等过程，还参与调控植物对环境胁迫的响应，如干旱、盐碱和温度等胁迫响应。目前，已鉴定与植物14-3-3蛋白互作的蛋白超过300个，说明14-3-3蛋白在植物生长发育过程中具有重要作用。本文主要总结了近年来植物14-3-3蛋白的结构及功能方面的研究进展。
Keyword ：
14-3-3蛋白 14-3-3蛋白 生长发育 生长发育 胁迫响应 胁迫响应
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| GB/T 7714 | 孙熔谦 , 晋欢欢 , 张静 et al. 植物14-3-3蛋白结构与功能的研究进展 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (02) : 145-152 . |
| MLA | 孙熔谦 et al. "植物14-3-3蛋白结构与功能的研究进展" . | 福建农林大学学报(自然科学版) 53 . 02 (2024) : 145-152 . |
| APA | 孙熔谦 , 晋欢欢 , 张静 , 王琴 , 张莉 . 植物14-3-3蛋白结构与功能的研究进展 . | 福建农林大学学报(自然科学版) , 2024 , 53 (02) , 145-152 . |
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| GB/T 7714 | Jiang, Bochen , Zhong, Zhenhui , Gu, Lianfeng et al. Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis (vol 9, pg 2042, 2023) [J]. | NATURE PLANTS , 2024 , 10 (1) : 192-192 . |
| MLA | Jiang, Bochen et al. "Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis (vol 9, pg 2042, 2023)" . | NATURE PLANTS 10 . 1 (2024) : 192-192 . |
| APA | Jiang, Bochen , Zhong, Zhenhui , Gu, Lianfeng , Zhang, Xueyang , Wei, Jiangbo , Ye, Chang et al. Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis (vol 9, pg 2042, 2023) . | NATURE PLANTS , 2024 , 10 (1) , 192-192 . |
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Translation initiation is a critical, rate-limiting step in protein synthesis. The eukaryotic translation initiation factor 4E (eIF4E) plays an essential role in this process. However, the mechanisms by which eIF4E-dependent translation initiation regulates plant growth and development remain not fully understood. In this study, we found that Arabidopsis eIF4E proteins are distributed in both the nucleus and cytoplasm, with only the cytoplasmic eIF4E being involved in the control of photoperiodic flowering. Genome-wide translation profiling using Ribo-tag sequencing reveals that eIF4E may regulate plant flowering by maintaining the homeostatic translation of components in the photoperiodic flowering pathway. eIF4E not only regulates the translation of flowering genes such as FLOWERING LOCUS T (FT) and FLOWERING LOCUS D (FLD) but also influences the translation of circadian genes like CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and PSEUDO-RESPONSE REGULATOR 9 (PRR9). Consistently, our results show that the eIF4E modulates the rhythmic oscillation of the circadian clock. Together, our study provides mechanistic insights into how the protein translation regulates multiple developmental processes in Arabidopsis, including the circadian clock and photoperiodic flowering.
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| GB/T 7714 | Zhang, Jing , Jin, Huanhuan , Chen, Yadi et al. The eukaryotic translation initiation factor eIF4E regulates flowering and circadian rhythm in Arabidopsis [J]. | PLANT JOURNAL , 2024 , 120 (1) : 123-138 . |
| MLA | Zhang, Jing et al. "The eukaryotic translation initiation factor eIF4E regulates flowering and circadian rhythm in Arabidopsis" . | PLANT JOURNAL 120 . 1 (2024) : 123-138 . |
| APA | Zhang, Jing , Jin, Huanhuan , Chen, Yadi , Jiang, Yonghong , Gu, Lianfeng , Lin, Guifang et al. The eukaryotic translation initiation factor eIF4E regulates flowering and circadian rhythm in Arabidopsis . | PLANT JOURNAL , 2024 , 120 (1) , 123-138 . |
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Protein-protein interaction (PPI) play a pivotal role in cellular signal transduction. The bimolecular fluorescence complementation (BiFC) assay offers a rapid and intuitive means to ascertain the localization and interactions of target proteins within living cells. BiFC is based on fluorescence complementation by reconstitution of a functional fluorescent protein by co-expression of N- and C-terminal fragments of this protein. When fusion proteins interact, the N- and C-terminal fragments come into close proximity, leading to the reconstitution of the fluorescent protein. In the conventional approach, the N-terminal and C-terminal fragments of the fluorescent protein are typically expressed using two separate vectors, which largely relies on the efficiency of the transformation of the two vectors in the same cells. Furthermore, issues of vector incompatibility can often result in loss of one plasmid. To address these challenges, we have developed novel dual-transgenic BiFC vectors, designed as pDTQs, derived from the previously published pDT1 vector. This set of BiFC vectors offers the following advantages: 1) Both fluorescent fusion proteins are expressed sequentially within a single vector, enhancing expression efficiency; 2) Independent promoters and terminators regulate the expression of the two proteins potentially mitigating vector compatibility issues; 3) A long linker is inserted between the fluorescent protein fragment and the gene of interest, facilitating the recombination of the fused fluorescent protein into an active form; 4) Four distinct types of fluorescent proteins, namely, EYFP, mVenus, mRFP1Q66T and mCherry are available for BiFC analysis. We assessed the efficiency of the pDTQs system by investigating the oligomerization of Arabidopsis CRY2 and CRY2-BIC2 interactions in N. benthamiana. Notably, the pDTQs were found to be applicable in rice, underscoring their potential utility across various plant species.
Keyword ：
bimolecular fluorescence complementation (BiFC) bimolecular fluorescence complementation (BiFC) EYFP EYFP mCherry mCherry mVenus mVenus protein-protein interaction (PPI) protein-protein interaction (PPI)
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| GB/T 7714 | Chen, Piaojuan , Ye, Meiling , Chen, Yadi et al. Dual-transgenic BiFC vector systems for protein-protein interaction analysis in plants [J]. | FRONTIERS IN GENETICS , 2024 , 15 . |
| MLA | Chen, Piaojuan et al. "Dual-transgenic BiFC vector systems for protein-protein interaction analysis in plants" . | FRONTIERS IN GENETICS 15 (2024) . |
| APA | Chen, Piaojuan , Ye, Meiling , Chen, Yadi , Wang, Qin , Wang, Qiongli , Zhong, Ming . Dual-transgenic BiFC vector systems for protein-protein interaction analysis in plants . | FRONTIERS IN GENETICS , 2024 , 15 . |
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NAC转录因子家族是大型植物特异性转录因子家族之一，广泛参与植物侧根生长、次生壁形成、叶片衰老、花器官形成、果实发育等生长发育过程，并响应干旱、寒冷、盐碱以及病原菌入侵等逆境胁迫，极大地帮助了植物在各种环境中更好的生存。综述了近年来植物NAC转录因子家族结构和功能的研究进展，以期促进对NAC转录因子调控网络的理解和更深入的研究。
Keyword ：
NAC转录因子 NAC转录因子 生长发育 生长发育 胁迫反应 胁迫反应
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| GB/T 7714 | 刘惠玲 , 江炳玉 , 张静 et al. 植物NAC转录因子的研究进展 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (06) : 742-753 . |
| MLA | 刘惠玲 et al. "植物NAC转录因子的研究进展" . | 福建农林大学学报(自然科学版) 53 . 06 (2024) : 742-753 . |
| APA | 刘惠玲 , 江炳玉 , 张静 , 王琴 , 张莉 . 植物NAC转录因子的研究进展 . | 福建农林大学学报(自然科学版) , 2024 , 53 (06) , 742-753 . |
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Energetic materials (EMs) have been widely employed in both military and civilian areas for nearly two centuries. The introduction of high-energy azide anions to assemble energetic metal-organic frameworks (EMOFs) is an efficient strategy to enhance energetic properties. However, azido-based EMOFs always suffer low stabilities to external mechanical stimulation. Herein, we employed an in situ hydrothermal reaction as a technique to refine azide anions with a neutral triazole-cyano-based ligand TrzAt (TrzAt = 2-(1H-1,2,4-triazol-1-yl)acetonitrile) to yield two tetrazole-based EMOFs, namely, [ZnBr(trmetz)](n) 1 and [Cd(trmetz)(2)](n) 2 (Htrmetz = 5-(1,2,4-triazol-1-ylmethyl)-1H-tetrazole). Compound 1 features a closely packed 2D layered network, while compound 2 exhibits a 3D architecture. With azide anions inlaid into a nitrogen-rich and chelating ligand in the EMOFs, compounds 1 and 2 present remarkable decomposition temperatures (T-dec >= 300 degrees C), low impact sensitivities (IS >= 32 J) and low friction sensitivities (FS >= 324 N). The calculated heat of detonation (Delta H-det) values of 1 and 2 are 3.496 and 4.112 kJ g(-1), respectively. In particular, the Delta H-det value of 2 is higher than that of traditional secondary explosives such as 2,4,6-trinitrotoluene (TNT, Delta H-det = 3.720 kJ g(-1)). These results indicate that EMOFs 1 and 2 may serve as potential replacements for traditional secondary explosives. This work provides a simple and effective strategy to obtain two EMOFs with satisfactory energy densities and reliable stabilities through an in situ hydrothermal technique for desensitization of azide anions.
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| GB/T 7714 | Xie, Hao-Hui , Weng, Jiao-Lin , Song, Ji-Xing et al. Refinement of sensitive azides via in situ generated azole-based metal-organic frameworks towards stable energetic materials [J]. | DALTON TRANSACTIONS , 2023 , 52 (40) : 14632-14639 . |
| MLA | Xie, Hao-Hui et al. "Refinement of sensitive azides via in situ generated azole-based metal-organic frameworks towards stable energetic materials" . | DALTON TRANSACTIONS 52 . 40 (2023) : 14632-14639 . |
| APA | Xie, Hao-Hui , Weng, Jiao-Lin , Song, Ji-Xing , Yang, Wen-Jing , Wang, Qin , Cui, Meng et al. Refinement of sensitive azides via in situ generated azole-based metal-organic frameworks towards stable energetic materials . | DALTON TRANSACTIONS , 2023 , 52 (40) , 14632-14639 . |
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