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学者姓名:郭子龙
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Abstract :
Magnesium (Mg) and calcium (Ca) are the most abundant divalent cations in plants. Cause their antagonistic interaction in plant cell, maintaining Ca-Mg balance is critical for optimal plant growth and development. However, the molecular mechanisms underlying the regulation of Ca-Mg balance remain poorly understood. In this study, we found that the expression of OsCAX1a was highly induced under high Ca:Mg ratio conditions in rice. Heterologous expression of OsCAX1a in yeast demonstrated that it enhances cytosolic Mg efficiency by mediating Ca efflux. Genetic knockout or overexpression of OsCAX1a in rice altered the Ca:Mg ratio and impaired growth performance. Collectively, our results indicate that OsCAX1a-mediated Ca efflux plays an important role in Mg homeostasis, providing a new insight for Ca-Mg balance in plant.
Keyword :
Calcium Calcium Magnesium Magnesium OsCAX1a OsCAX1a Transport Transport
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| GB/T 7714 | Tian, Xin-Yue , Cao, Hong-Rui , Li, Qi-Rong et al. OsCAX1a-Dependent Ca-Mg Balance Is Required for Optimal Growth in Rice [J]. | RICE , 2025 , 18 (1) . |
| MLA | Tian, Xin-Yue et al. "OsCAX1a-Dependent Ca-Mg Balance Is Required for Optimal Growth in Rice" . | RICE 18 . 1 (2025) . |
| APA | Tian, Xin-Yue , Cao, Hong-Rui , Li, Qi-Rong , Wu, Xiao , Liu, Bing-Sheng , Guo, Zi-Long et al. OsCAX1a-Dependent Ca-Mg Balance Is Required for Optimal Growth in Rice . | RICE , 2025 , 18 (1) . |
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Drought stress significantly impacts global rice production, highlighting the critical need to understand the genetic basis of drought resistance in rice. Here, through a genome-wide association study, we reveal that natural variations in DROUGHT RESISTANCE GENE 9 (DRG9), encoding a double-stranded RNA (dsRNA) binding protein, contribute to drought resistance. Under drought stress, DRG9 condenses into stress granules (SGs) through liquid-liquid phase separation via a crucial alpha-helix. DRG9 recruits the mRNAs of OsNCED4, a key gene for the biosynthesis of abscisic acid, into SGs and protects them from degradation. In drought-resistant DRG9 allele, natural variations in the coding region, causing an amino acid substitution (G267F) within the zinc finger domain, increase DRG9's binding ability to OsNCED4 mRNA and enhance drought resistance. Introgression of the drought-resistant DRG9 allele into the elite rice Huanghuazhan significantly improves its drought resistance. Thus, our study underscores the role of a dsRNA-binding protein in drought resistance and its promising value in breeding drought-resistant rice. Drought is one of the major abiotic stresses affecting rice growth and development. Here, the authors identify a dsRNA-binding protein positively regulates rice drought resistance through promoting stability of OsNCED4 mRNAs, transcript of a key gene for the biosynthesis of abscisic acid, via protein phase separation.
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| GB/T 7714 | Wang, Huaijun , Ye, Tiantian , Guo, Zilong et al. A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice [J]. | NATURE COMMUNICATIONS , 2024 , 15 (1) . |
| MLA | Wang, Huaijun et al. "A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice" . | NATURE COMMUNICATIONS 15 . 1 (2024) . |
| APA | Wang, Huaijun , Ye, Tiantian , Guo, Zilong , Yao, Yilong , Tu, Haifu , Wang, Pengfei et al. A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice . | NATURE COMMUNICATIONS , 2024 , 15 (1) . |
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| GB/T 7714 | Wang, Huaijun , Ye, Tiantian , Guo, Zilong et al. A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice (vol 15, 2514, 2024) [J]. | NATURE COMMUNICATIONS , 2024 , 15 (1) . |
| MLA | Wang, Huaijun et al. "A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice (vol 15, 2514, 2024)" . | NATURE COMMUNICATIONS 15 . 1 (2024) . |
| APA | Wang, Huaijun , Ye, Tiantian , Guo, Zilong , Yao, Yilong , Tu, Haifu , Wang, Pengfei et al. A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice (vol 15, 2514, 2024) . | NATURE COMMUNICATIONS , 2024 , 15 (1) . |
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为进一步挖掘控制水稻抗倒伏的基因,以524份水稻种质资源为材料,采用GWAS(genome-wide association analysis)鉴定与抗倒伏性状显著关联的位点qRLG7,通过基因表达水平分析和候选基因关联分析确定调控水稻抗倒伏性的候选基因,在分析候选基因的表达特征和启动子区自然变异基础上利用CRISPR-Cas9技术构建2个候选基因的突变体家系,考察转基因材料的抗倒伏性状。结果显示,编码肌醇-1-单磷酸酶的2个串联排列基因LOC_Os07g37220和LOC_Os07g37230在离茎秆基部5 cm节间中的表达量显著高于其余候选基因,而且这2个候选基因的表达量在极端抗倒伏和易倒伏2组材料中存在极显著差异;通过候选基因关联分析发现这2个基因启动子区的SNP与离基部5 cm茎秆直径(CD5)的表型值显著关联,且基于显著关联SNP的2种单倍型的CD5表型值存在显著差异,这2个候选基因的2种单倍型启动子活性检测结果也表明2种单倍型之间存在极显著差异;2个基因的功能缺失突变体植株在基部节间抗折力、茎秆厚度、茎秆直径以及株高和穗质量等性状方面与野生型对照存在显著差异。结果表明,候选基因LOC_Os07g37220和LOC_Os07g37230具有一定的抗倒伏功能。
Keyword :
候选基因 候选基因 关联分析 关联分析 抗倒伏 抗倒伏 水稻 水稻 肌醇-1-单磷酸酶(IMP) 肌醇-1-单磷酸酶(IMP)
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| GB/T 7714 | 西若曲宗 , 向登豪 , 郭子龙 et al. 水稻抗倒伏候选基因的鉴定与功能分析 [J]. | 华中农业大学学报 , 2024 , 43 (02) : 75-84 . |
| MLA | 西若曲宗 et al. "水稻抗倒伏候选基因的鉴定与功能分析" . | 华中农业大学学报 43 . 02 (2024) : 75-84 . |
| APA | 西若曲宗 , 向登豪 , 郭子龙 , 姚义龙 , 董发明 , 熊立仲 . 水稻抗倒伏候选基因的鉴定与功能分析 . | 华中农业大学学报 , 2024 , 43 (02) , 75-84 . |
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Taking advantage of natural variation promotes our understanding of phenotypic diversity and trait evolution, ultimately accelerating plant breeding, in which the identification of causal variations is critical. To date, sequence variations in the coding region and transcription level polymorphisms caused by variations in the promoter have been prioritized. An upstream open reading frame (uORF) in the 5 ' untranslated region (5 ' UTR) regulates gene expression at the posttranscription or translation level. In recent years, studies have demonstrated that natural uORF variations shape phenotypic diversity. This opinion article highlights recent researches and speculates on future directions for natural uORF variation in plants.
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| GB/T 7714 | Wang, Jiangen , Liu, Juhong , Guo, Zilong . Natural uORF variation in plants [J]. | TRENDS IN PLANT SCIENCE , 2024 , 29 (3) : 290-302 . |
| MLA | Wang, Jiangen et al. "Natural uORF variation in plants" . | TRENDS IN PLANT SCIENCE 29 . 3 (2024) : 290-302 . |
| APA | Wang, Jiangen , Liu, Juhong , Guo, Zilong . Natural uORF variation in plants . | TRENDS IN PLANT SCIENCE , 2024 , 29 (3) , 290-302 . |
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Symbiotic nitrogen fixation (SNF) provides much of the N utilized by leguminous plants throughout growth and development. Legumes may simultaneously establish symbiosis with different taxa of microbial symbionts. Yet, the mechanisms used to steer associations toward symbionts that are most propitious across variations in soil types remain mysterious. Here, we demonstrate that GmRj2/Rfg1 is responsible for regulating symbiosis with multiple taxa of soybean symbionts. In our experiments, the GmRj2/Rfg1SC haplotype favored association with Bradyrhizobia, which is mostly distributed in acid soils, whereas the GmRj2/Rfg1HH haplotype and knockout mutants of GmRj2/Rfg1SC associated equally with Bradyrhizobia and Sinorhizobium. Association between GmRj2/Rfg1 and NopP, furthermore, appeared to be involved in symbiont selection. Furthermore, geographic distribution analysis of 1,821 soybean accessions showed that GmRj2/Rfg1SC haplotypes were enriched in acidic soils where Bradyrhizobia were the dominant symbionts, whereas GmRj2/ Rfg1HH haplotypes were most prevalent in alkaline soils dominated by Sinorhizobium, and neutral soils harbored no apparent predilections toward either haplotype. Taken together, our results suggest that GmRj2/Rfg1 regulates symbiosis with different symbionts and is a strong determinant of soybean adaptability across soil regions. As a consequence, the manipulation of the GmRj2/Rfg1 genotype or application of suitable symbionts according to the haplotype at the GmRj2/Rfg1 locus might be suitable strategies to explore for increasing soybean yield through the management of SNF.
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| GB/T 7714 | Li, Yanjun , Wang, Cunhu , Zheng, Lei et al. Natural variation of GmRj2/Rfg1 determines symbiont differentiation in soybean [J]. | CURRENT BIOLOGY , 2023 , 33 (12) : 2478-, . |
| MLA | Li, Yanjun et al. "Natural variation of GmRj2/Rfg1 determines symbiont differentiation in soybean" . | CURRENT BIOLOGY 33 . 12 (2023) : 2478-, . |
| APA | Li, Yanjun , Wang, Cunhu , Zheng, Lei , Ma, Wenjing , Li, Mingjia , Guo, Zilong et al. Natural variation of GmRj2/Rfg1 determines symbiont differentiation in soybean . | CURRENT BIOLOGY , 2023 , 33 (12) , 2478-, . |
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The execution of specific processing protocols endows Wuyi rock tea with distinctive qualities produced through signature metabolic processes. In this work, tea leaves were collected before and after each of three processing stages for both targeted and untargeted metabolomic analysis. Metabolic profiles of processing stages through each processing stage of rotation, pan-firing and roasting were studied. Overall, 614 metabolites were significantly altered, predominantly through nitrogen- enriching (N) pathways. Roasting led to the enrichment of 342 N metabolites, including 34 lipids, 17 organic acids, 32 alkaloids and 25 amino acids, as well as secondary derivatives beneficial for tea quality. This distinctive shift towards enrichment of N metabolites strongly supports concluding that this directed accumulation of N metabolites is how each of the three processing stages endows Wuyi rock tea with singular quality.
Keyword :
metabolome metabolome N-contained metabolites N-contained metabolites process process tea quality tea quality Wuyi rock tea Wuyi rock tea
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| GB/T 7714 | Liu, Yang , Zhuo, Zuopin , Tian, Jing et al. Directed Accumulation of Nitrogen Metabolites through Processing Endows Wuyi Rock Tea with Singular Qualities [J]. | MOLECULES , 2022 , 27 (10) . |
| MLA | Liu, Yang et al. "Directed Accumulation of Nitrogen Metabolites through Processing Endows Wuyi Rock Tea with Singular Qualities" . | MOLECULES 27 . 10 (2022) . |
| APA | Liu, Yang , Zhuo, Zuopin , Tian, Jing , Liu, Bei , Shi, Chen , Xu, Ruineng et al. Directed Accumulation of Nitrogen Metabolites through Processing Endows Wuyi Rock Tea with Singular Qualities . | MOLECULES , 2022 , 27 (10) . |
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Phosphorus (P) is an essential element for all organisms. Because P fertilizers are a non-renewable resource and high fixation in soils, sustainable agriculture requires researchers to improve crop P acquisition efficiency. Here, we report a strong association signal at a locus of CPU1 (component of phosphorus uptake 1), from a genome-wide association study of P acquisition efficiency in a soybean core collection grown in the field. A SEC12-like gene, GmPHF1, is identified as the causal gene for CPU1. GmPHF1 facilitates the ER (endoplasmic reticulum) exit of the phosphate transporter, GmPT4, to the plasma membrane of root epidermal cells. A common SNP in an upstream open reading frame (uORF) of GmPHF1, which alters the abundance of GmPHF1 in a tissue-specific manner, contributes to P acquisition diversity in soybean. A natural genetic variation conditions diversity in soybean P acquisition, which can be used to develop P-efficient soybean genotypes. Improving phosphorus (P) acquisition efficiency is important for sustainable agriculture. Here, the authors report a natural variation in an upstream open reading frame of a SEC12-like gene GmPHF1 is crucial for protein abundance and spatial distribution of GmPHF1, which contribute to P acquisition diversity in soybean.
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| GB/T 7714 | Guo, Zilong , Cao, Hongrui , Zhao, Jing et al. A natural uORF variant confers phosphorus acquisition diversity in soybean [J]. | NATURE COMMUNICATIONS , 2022 , 13 (1) . |
| MLA | Guo, Zilong et al. "A natural uORF variant confers phosphorus acquisition diversity in soybean" . | NATURE COMMUNICATIONS 13 . 1 (2022) . |
| APA | Guo, Zilong , Cao, Hongrui , Zhao, Jing , Bai, Shuang , Peng, Wenting , Li, Jian et al. A natural uORF variant confers phosphorus acquisition diversity in soybean . | NATURE COMMUNICATIONS , 2022 , 13 (1) . |
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Symbiotic nitrogen fixation provides large amounts of nitrogen for global agricultural systems with little environmental or economic costs. The basis of symbiosis is the nutrient exchange occurring between legumes and rhizobia, but key regulators controlling nutrient exchange are largely unknown. Here, we reveal that magnesium (Mg), an important nutrient factor that preferentially accumulates in inner cortical cells of soybean nodules, shows the most positive correlation with nodule carbon (C) import and nitrogen (N) export. We further identified a pair of Mg transporter genes, GmMGT4 and GmMGT5, that are specifically expressed in the nodule cortex, modulating both nodule Mg import and C-N transport processes. The GmMGT4&5-dependent Mg import activates the activity of a plasmodesmata-located beta-1,3-glucanase GmBG2 and consequently keeps plasmodesmata permeable for C-N transport in nodule inner cortical cells. Our studies discovered an important regulating pathway for host plants fine-tuning nodule C-N trading to achieve optimal growth, which may be helpful for optimizing nutrient management for soybean production.
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| GB/T 7714 | Cao, Hong-Rui , Peng, Wen-Ting , Nie, Miao-Miao et al. Carbon-nitrogen trading in symbiotic nodules depends on magnesium import [J]. | CURRENT BIOLOGY , 2022 , 32 (20) : 4337-, . |
| MLA | Cao, Hong-Rui et al. "Carbon-nitrogen trading in symbiotic nodules depends on magnesium import" . | CURRENT BIOLOGY 32 . 20 (2022) : 4337-, . |
| APA | Cao, Hong-Rui , Peng, Wen-Ting , Nie, Miao-Miao , Bai, Shuang , Chen, Chun-Qu , Liu, Qian et al. Carbon-nitrogen trading in symbiotic nodules depends on magnesium import . | CURRENT BIOLOGY , 2022 , 32 (20) , 4337-, . |
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Salinity is one of the main adverse environmental factors severely inhibiting rice growth and decreasing grain productivity. Developing rice varieties with salt tolerance (ST) is one of the most economical approaches to cope with salinity stress. In this study, the salt tolerance of 220 rice accessions from rice diversity panel l (RDP1), representing five subpopulations, were evaluated based on 16 ST indices at both seedling and reproductive stages under salt stress. An apparent inconsistency was found for ST between the two stages. Through a gene-based/tightly linked genome-wide association study with 201,332 single nucleotide polymorphisms (SNPs) located within genes and their flanking regions were used, a total of 214 SNPs related to 251 genes, significantly associated with 16 ST-related indices, were detected at both stages. Eighty-two SNPs with low frequency favorable (LFF) alleles in the population were proposed to hold high breeding potential in improving rice ST. Fifty-four rice accessions collectively containing all these LFF alleles were identified as donors of these alleles. Through the integration of meta-quantitative trait locus (QTL) for ST and the response patterns of differential expression genes to salt stress, thirty-eight candidate genes were suggested to be involved in the regulation of rice ST. In total, the present study provides valuable information for further characterizing ST-related genes and for breeding ST varieties across whole developmental stages through marker-assisted selection (MAS).
Keyword :
developmental stages developmental stages genome-wide association study (GWAS) genome-wide association study (GWAS) natural accessions natural accessions Oryza sativa L Oryza sativa L salt tolerance salt tolerance
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| GB/T 7714 | Chen, Gang , Hu, Keming , Zhao, Jianhua et al. Genome-Wide Association Analysis for Salt-Induced Phenotypic and Physiologic Responses in Rice at Seedling and Reproductive Stages [J]. | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
| MLA | Chen, Gang et al. "Genome-Wide Association Analysis for Salt-Induced Phenotypic and Physiologic Responses in Rice at Seedling and Reproductive Stages" . | FRONTIERS IN PLANT SCIENCE 13 (2022) . |
| APA | Chen, Gang , Hu, Keming , Zhao, Jianhua , Guo, Feifei , Shan, Wenfeng , Jiang, Qiuqing et al. Genome-Wide Association Analysis for Salt-Induced Phenotypic and Physiologic Responses in Rice at Seedling and Reproductive Stages . | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
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