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Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor SCIE
期刊论文 | 2025 , 12 (1) | SCIENTIFIC DATA
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Arhopalus unicolor is a carrier of the pine wood nematode (PWN), which causes pine wilt disease, killing pine trees and causing considerable economic and environmental losses. While the A. unicolor mitochondrial genome has been published, a high-quality genome assembly and annotation of A. unicolor is not yet available. To address this, we assembled a chromosome-level reference genome assembly of A. unicolor with a combination of Illumina, PacBio, and Hi-C sequencing technologies. The final genome size was determined to be 1268.11 Mb, with a GC% of 32.44%, and the scaffold N50 value was 19.30 Mb. A total of 98.77% of the assembled sequences mapped to 10 pseudochromosomes, and BUSCO analysis revealed high completeness, with 97.15% gene coverage. Furthermore, the genome contains 71.74% repeat elements and encompasses 16,450 predicted protein-coding genes. This genome sequence of A. unicolor will be a valuable resource for understanding the genetics and evolutionary history of this species and for developing effective management strategies for this PWN carrier.

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GB/T 7714 Zou, Xiangying , Zeng, Liqiong , You, Xingzao et al. Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor [J]. | SCIENTIFIC DATA , 2025 , 12 (1) .
MLA Zou, Xiangying et al. "Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor" . | SCIENTIFIC DATA 12 . 1 (2025) .
APA Zou, Xiangying , Zeng, Liqiong , You, Xingzao , Zhong, Jinghui , Tang, Chensheng , Zheng, Zhaofei et al. Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor . | SCIENTIFIC DATA , 2025 , 12 (1) .
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Arginine accumulation suppresses heat production during fermentation of the biocontrol fungus Beauveria bassiana SCIE
期刊论文 | 2025 , 91 (3) | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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Beauveria bassiana (Bb) is one of the most widely used biocontrol agents, and its products constitute more than one-third of the global market share of fungal insecticides. Solid-state fermentation (SSF) is widely used in the production of Beauve ria bassiana (Bb) because of its economic practicality and high production efficiency . However, the heat generated during fermentation can sharply reduce both the yield and quality of Bb, and current industrial methods to mitigate high temperatures during fermentation are inadequate, leading to increased production costs. Thus, exploring the underlying mechanism of how heat is produced by Bb is crucial for improving the SSF procedure and yield. This study employed multiomics data analysis of Bb during SSF to explore the relationships between fungal fermentation and environmental factors. We found that the heat production period for SSF was 12 hours to 48 hours post-inoculation. To further explore the underlying mechanism during this heating period, we identified 454 temperature-correlated metabolites (TCMs) and 1,994 temperature-correlated genes (TCGs). Annotations of the above TCMs and TCGs revealed significant enrichment in the arginine biosynthesis pathway; specifically, the expression level of glutamine synthetase, a TCG, decreased with fermentation time, whereas the expression levels of the TCGs L-arginine and L-glutamine increased with fermentation time, and glutamine synthetase and L-glutamine in the arginine biosynthesis pathway cycle produced the end prod uct L-arginine. Furthermore, when the substrates of the SSF were treated with exoge nous arginine, the temperature peak of the SSF significantly decreased with increasing concentration of exogenously added arginine.

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arginine arginine Beauveria bassiana Beauveria bassiana heating period heating period multiomics multiomics solid-state fermentation solid-state fermentation

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GB/T 7714 Wu, Tong , Zhan, Fangfang , Zeng, Liqiong et al. Arginine accumulation suppresses heat production during fermentation of the biocontrol fungus Beauveria bassiana [J]. | APPLIED AND ENVIRONMENTAL MICROBIOLOGY , 2025 , 91 (3) .
MLA Wu, Tong et al. "Arginine accumulation suppresses heat production during fermentation of the biocontrol fungus Beauveria bassiana" . | APPLIED AND ENVIRONMENTAL MICROBIOLOGY 91 . 3 (2025) .
APA Wu, Tong , Zhan, Fangfang , Zeng, Liqiong , Sun, Yanli , Fu, Shihui , Fang, Yu et al. Arginine accumulation suppresses heat production during fermentation of the biocontrol fungus Beauveria bassiana . | APPLIED AND ENVIRONMENTAL MICROBIOLOGY , 2025 , 91 (3) .
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Phosphorus deficiency suppresses the trans-zeatin riboside accumulation by up-regulating CeIPT5, promoting root elongation and phosphorus absorption efficiency of Casuarina equisetifolia SCIE
期刊论文 | 2025 , 45 (6) | TREE PHYSIOLOGY
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Phosphorus (P) deficiency is critical to the renewal barrier of she-oak (Casuarina equisetifolia), an important tree species used for coastal protection. However, the response of she-oak to P deficiency remains unclear. In this study, we compared the phenotypes of two she-oak cultivars, the P deficiency-sensitive 'Chihu219' and the insensitive 'Chihu397', and found that P deficiency significantly increased root growth, P concentration and phosphorus absorption efficiency (PAE) in Chihu219, but not in Chihu397. We also analyzed the transcriptome and metabolome of these cultivars under different P conditions and showed that trans-zeatin riboside (tZR) levels were highly suppressed by P deficiency in Chihu219, but not in Chihu397. Furthermore, exogenous tZR suppressed both root P concentration and PAE while promoting P-use efficiency. We also identified CeIPT5 (isopentenyltransferase 5) as a key regulatory gene of tZR biosynthesis and found that its expression was more highly induced by P deficiency in Chihu219 than in Chihu397. We also showed that overexpression of CeIPT5 in insensitive she-oak lines reduced tZR concentration and increased root P concentration compared with the vector control. Taken together, P deficiency can greatly reduce tZR accumulation in P deficiency-insensitive she-oak at least by activating the tZR accumulation regulatory gene, CeIPT5, thereby promoting root elongation and P concentration. This study not only provides a genetic basis for enhancing PAE in woody plants but also establishes a theoretical basis for optimizing root structure and improving nutrient utilization efficiency, thereby promoting sustainable forestry development.

Keyword :

beach she-oak beach she-oak cytokinin cytokinin isopentenyltransferases 5 isopentenyltransferases 5 phosphate limitation phosphate limitation phosphate uptake phosphate uptake root growth root growth

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GB/T 7714 Jiang, Lijuan , Yang, Hua , Du, Yunshuang et al. Phosphorus deficiency suppresses the trans-zeatin riboside accumulation by up-regulating CeIPT5, promoting root elongation and phosphorus absorption efficiency of Casuarina equisetifolia [J]. | TREE PHYSIOLOGY , 2025 , 45 (6) .
MLA Jiang, Lijuan et al. "Phosphorus deficiency suppresses the trans-zeatin riboside accumulation by up-regulating CeIPT5, promoting root elongation and phosphorus absorption efficiency of Casuarina equisetifolia" . | TREE PHYSIOLOGY 45 . 6 (2025) .
APA Jiang, Lijuan , Yang, Hua , Du, Yunshuang , Zheng, Zhaoliang , Ding, Shanshan , Zhang, Xinyan et al. Phosphorus deficiency suppresses the trans-zeatin riboside accumulation by up-regulating CeIPT5, promoting root elongation and phosphorus absorption efficiency of Casuarina equisetifolia . | TREE PHYSIOLOGY , 2025 , 45 (6) .
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CeJAZ3 suppresses longifolene accumulation in Casuarina equisetifolia, affecting the host preference of Anoplophora chinensis SCIE
期刊论文 | 2025 , 81 (4) , 2202-2214 | PEST MANAGEMENT SCIENCE
WoS CC Cited Count: 1
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BACKGROUNDCasuarina equisetifolia, a crucial species of coastal windbreaks, is highly susceptible to infestation by Anoplophora chinensis. This stem-boring pest poses a significant threat to the health and sustainability of Casuarina equisetifolia forests. Understanding the molecular mechanisms underlying the host preference of A. chinensis to Casuarina equisetifolia is essential for developing effective pest management strategies.RESULTSThrough field surveys, we identified two cultivars of Casuarina equisetifolia that exhibited differing levels of host preference for A. chinensis. Further analysis of multi-omics data (phenomics, transcriptomics, and metabolomics) from these cultivars revealed that longifolene plays a significant role in attracting A. chinensis to Casuarina equisetifolia. Additionally, the jasmonic acid (JA) signaling pathway was found to suppress longifolene accumulation, primarily through the interaction between the jasmonate ZIM-domain (JAZ) proteins and the terpene synthase (TPS) gene. Moreover, we identified a critical JAZ component, CeJAZ3, whose overexpression led to the down-regulation of TPS expression levels and, consequently, a reduced release of longifolene.CONCLUSIONWe confirmed that the negative regulator of host preference, CeJAZ3, in the JA signaling pathway can suppress the expression of TPSs, thereby down-regulating the accumulation of longifolene in Casuarina equisetifolia and indirectly suppressing the attraction of host plants to A. chinensis, which provides a basis for the integrated management of A. chinensis. (c) 2024 Society of Chemical Industry.

Keyword :

Anoplophora chinensis Anoplophora chinensis Casuarina equisetifolia Casuarina equisetifolia host preference host preference integrated pest management integrated pest management JAZ JAZ longifolene longifolene

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GB/T 7714 Yang, Hua , Jiang, Lijuan , Bao, Xiaochun et al. CeJAZ3 suppresses longifolene accumulation in Casuarina equisetifolia, affecting the host preference of Anoplophora chinensis [J]. | PEST MANAGEMENT SCIENCE , 2025 , 81 (4) : 2202-2214 .
MLA Yang, Hua et al. "CeJAZ3 suppresses longifolene accumulation in Casuarina equisetifolia, affecting the host preference of Anoplophora chinensis" . | PEST MANAGEMENT SCIENCE 81 . 4 (2025) : 2202-2214 .
APA Yang, Hua , Jiang, Lijuan , Bao, Xiaochun , Liu, Haolan , Xu, Qianle , Yao, Xingliang et al. CeJAZ3 suppresses longifolene accumulation in Casuarina equisetifolia, affecting the host preference of Anoplophora chinensis . | PEST MANAGEMENT SCIENCE , 2025 , 81 (4) , 2202-2214 .
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高压注射2%甲维盐乳油对马尾松林多光谱特征的影响
期刊论文 | 2025 , 52 (01) , 57-63 | 福建林业科技
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于2022年在福建省三明市沙县官庄国有林场,对14年生的马尾松纯林(2代种子园)开展树干高压注射2%甲维盐(EB)乳油试验(以不注射作为对照),选用大疆精灵4RTK作为飞行平台,首先提取注射前不同马尾松植株的健康度相关光谱特征,综合人工智能模型,将样地中所有单株分成3类:健康、(线虫)轻度感染与(线虫)重度感染类群;同时以与健康度高度正相关的NDVI(归一化植被指数)值、以及与松树感病程度负相关的NIR、Red Edge为核心,研究注射2%EB乳油90、120、180 d后3个类群健康度和松树感病程度的变化。结果表明:(1)注射2%EB乳油后马尾松林分枯死率比对照组下降80%。(2)与对照相比,注射2%EB乳油180 d后,马尾松植株的整体健康度显著提升,表现为NDVI和简单比率值指数(MSR)值比对照均有极显著的提升(P<0.01)。NIR、RedEdge、NDVI值与注射前相比,重度感染类群植株有显著提升,分别为1.18、1.36、1.55倍(P<0.05);健康类群和轻度感染类群植株也有不同程度提升,分别为1.42、1.20、1.24倍(P<0.05)和1.08、1.09、1.22倍(P<0.05);与对照相比,健康、(线虫)轻度感染与(线虫)重度感染类群植株显著提升1.17、1.34、1.39倍(P<0.05)。(3)时间尺度上,对照组的NIR、Red Edge、NDVI值均无显著变化或稍有下降。说明树干高压注射2%EB乳油能够有效改善马尾松林的健康状况,并有效控制松材线虫病的危害,与林间马尾松植株枯死情况观察结果一致。

Keyword :

无人机近地遥感 无人机近地遥感 松材线虫病 松材线虫病 树干高压注射 树干高压注射 甲维盐 甲维盐 马尾松 马尾松

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GB/T 7714 董玉光 , 郭芷晴 , 蔡守平 et al. 高压注射2%甲维盐乳油对马尾松林多光谱特征的影响 [J]. | 福建林业科技 , 2025 , 52 (01) : 57-63 .
MLA 董玉光 et al. "高压注射2%甲维盐乳油对马尾松林多光谱特征的影响" . | 福建林业科技 52 . 01 (2025) : 57-63 .
APA 董玉光 , 郭芷晴 , 蔡守平 , 曾丽琼 , 黄丹泠 , 陈志云 et al. 高压注射2%甲维盐乳油对马尾松林多光谱特征的影响 . | 福建林业科技 , 2025 , 52 (01) , 57-63 .
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GLR36T807I Mutation of Casuarina equisetifoli Is Associated With a Decreased JA Response to Insect Feeding by Lymantria xylina SCIE
期刊论文 | 2025 , 48 (5) , 3185-3198 | PLANT CELL AND ENVIRONMENT
WoS CC Cited Count: 2
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Lymantria xylina is the most important defoliator, damaging the effective coastal windbreak tree species Casuarina equisetifolia. However, the underlying genetic mechanisms through which C. equisetifolia responds to L. xylina attacks remain unknown. Here, we compared the transcriptional, phytohormone and metabolic differences between susceptible (S) and resistant (R) C. equisetifolia cultivars in response to L. xylina feeding. The main L. xylina-induced resistance in C. equisetifolia was a jasmonate (JA) response and JA synthesis was highly induced by L. xylina feeding at both the transcriptional and metabolic levels, thus promoting flavonoid accumulation. The JA response was highly activated by L. xylina feeding on the R but not in the S cultivar, although the JA signalling pathway was intact in both cultivars. We found a single amino acid mutation in the homologues of glutamate receptor-like protein 3.6 (CeGLR3.6T807I) in the S cultivar. Compared with the GLR3.6 homologues in the R cultivar, phosphorylation of CeGLR3.6T807I was not induced by insect feeding, leading to a decreased JA response in the S cultivar. Collectively, this study provides new insights into the function of CeGLR3.6 in regulating the JA response of C. equisetifolia to L. xylina feeding.

Keyword :

Casuarina equisetifolia Casuarina equisetifolia flavonoids flavonoids glutamate receptor-like protein 3.6 (GLR3.6) glutamate receptor-like protein 3.6 (GLR3.6) jasmonic acid jasmonic acid Lymantria xylina Lymantria xylina phosphorylation phosphorylation

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GB/T 7714 Chang, Huan , Li, Chengli , Zhu, Tengfei et al. GLR36T807I Mutation of Casuarina equisetifoli Is Associated With a Decreased JA Response to Insect Feeding by Lymantria xylina [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (5) : 3185-3198 .
MLA Chang, Huan et al. "GLR36T807I Mutation of Casuarina equisetifoli Is Associated With a Decreased JA Response to Insect Feeding by Lymantria xylina" . | PLANT CELL AND ENVIRONMENT 48 . 5 (2025) : 3185-3198 .
APA Chang, Huan , Li, Chengli , Zhu, Tengfei , Cai, Shouping , Chen, Jie , Zhan, Fangfang et al. GLR36T807I Mutation of Casuarina equisetifoli Is Associated With a Decreased JA Response to Insect Feeding by Lymantria xylina . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (5) , 3185-3198 .
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Comparative Transcriptomics Unveils Pathogen-Specific mTOR Pathway Modulation in Monochamus alternatus Infected with Entomopathogenic Fungi SCIE
期刊论文 | 2025 , 16 (10) | INSECTS
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Pine wilt disease (PWD), transmitted by Monochamus alternatus (JPS), poses a severe threat to global pine forests. Although the entomopathogenic fungi Beauveria bassiana (Bb) and Metarhizium anisopliae (Ma) represent environmentally friendly biocontrol alternatives, their practical application is limited by inconsistent field performance and an incomplete understanding of host-pathogen interactions. We employed dual RNA-seq at the critical 48 h infection time point to systematically compare the transcriptional responses between JPS and Bb/Ma during infection. Key findings revealed distinct infection strategies: Bb preferentially induced autophagy pathways and modulated host carbohydrate metabolism to facilitate nutrient acquisition, triggering corresponding tissue degradation responses in JPS. In contrast, Ma primarily co-opted host amino acid and sugar metabolic pathways for biosynthetic processes, eliciting a stronger immune defense activation in JPS. Notably, the mTOR signaling pathway was identified as a key regulator of the differential host responses to various entomopathogenic fungi. Further functional validation-specifically, the application of a chemical inhibitor and RNAi targeting mTOR in JPS-confirmed that mTOR inhibition selectively enhanced Bb-induced mortality in JPS without affecting Ma virulence. Our findings reveal the molecular determinants of host-pathogen specificity in PWD biological control and indicate that mTOR regulation could serve as an effective strategy to improve fungal pesticide performance.

Keyword :

Beauveria bassiana Beauveria bassiana dual RNA-seq dual RNA-seq Metarhizium anisopliae Metarhizium anisopliae Monochamus alternatus Monochamus alternatus mTOR pathway mTOR pathway pine wilt disease pine wilt disease

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GB/T 7714 Guan, Haoran , He, Jinghong , Zhang, Chuanyu et al. Comparative Transcriptomics Unveils Pathogen-Specific mTOR Pathway Modulation in Monochamus alternatus Infected with Entomopathogenic Fungi [J]. | INSECTS , 2025 , 16 (10) .
MLA Guan, Haoran et al. "Comparative Transcriptomics Unveils Pathogen-Specific mTOR Pathway Modulation in Monochamus alternatus Infected with Entomopathogenic Fungi" . | INSECTS 16 . 10 (2025) .
APA Guan, Haoran , He, Jinghong , Zhang, Chuanyu , Shan, Ruiyang , Chen, Haoyuan , Wu, Tong et al. Comparative Transcriptomics Unveils Pathogen-Specific mTOR Pathway Modulation in Monochamus alternatus Infected with Entomopathogenic Fungi . | INSECTS , 2025 , 16 (10) .
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Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics SCIE
期刊论文 | 2024 , 80 (8) , 3873-3883 | PEST MANAGEMENT SCIENCE
WoS CC Cited Count: 1
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BACKGROUND: Pine wood nematode (PWN; Bursaphelenchus xylophilus) is the causative agent of pine wilt disease (PWD), which is considered the most dangerous biohazard to conifer trees globally. The transmission of PWN relies on insect vectors, particularly the Japanese pine sawyer (JPS; Monochamus alternatus). However, the molecular mechanism underlying PWN-JPS assembly remains largely unknown. RESULTS: Here, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full-length reference transcriptome for analyzing next-generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN-carrying associate genes (beta) were nominated in JPS by using the reported genomes of PWN and non-PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with beta, correlated with the PCA of JPS were nominated by RNA-Seq, and found that fatty acid beta-oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid beta-oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid beta-oxidation rate and increased PCA of JPS. CONCLUSIONS: In conclusion, MaCPT1 was able to decrease the PWN-JPS assembly formation through the fatty acid beta-oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS. (c) 2024 Society of Chemical Industry.

Keyword :

fatty acid beta-oxidation fatty acid beta-oxidation genetic mechanism genetic mechanism Japanese pine sawyer (Monochamus alternatus) Japanese pine sawyer (Monochamus alternatus) pine wilt disease pine wilt disease pine wood nematode (Bursaphelenchus xylophilus) pine wood nematode (Bursaphelenchus xylophilus) PWN carrying amount PWN carrying amount

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GB/T 7714 Zou, Xiangying , Jia, Jiayu , Zhu, Tengfei et al. Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics [J]. | PEST MANAGEMENT SCIENCE , 2024 , 80 (8) : 3873-3883 .
MLA Zou, Xiangying et al. "Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics" . | PEST MANAGEMENT SCIENCE 80 . 8 (2024) : 3873-3883 .
APA Zou, Xiangying , Jia, Jiayu , Zhu, Tengfei , Cai, Shouping , He, Yiman , Su, Shunde et al. Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics . | PEST MANAGEMENT SCIENCE , 2024 , 80 (8) , 3873-3883 .
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盐胁迫下接种内生真菌对短枝木麻黄幼苗生长的影响
期刊论文 | 2024 , 30 (04) , 777-785 | 应用与环境生物学报
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为明晰盐分胁迫下内生真菌对宿主根系生长的影响,将一年生短枝木麻黄(Casuarina equisetifolia)幼苗栽植到不同盐度(质量分数为0、3‰、6‰、9‰)土壤中,以添加菌株培养基(CK)为对照,研究接种耐盐性菌株葡萄座腔菌Botryosphaeria Z1(Z1)、炭团菌Hypoxylon Y6(Y6)及其混合菌剂(Z1+Y6)对其宿主幼苗根系形态与活力、养分吸收以及苗高、地径和根冠比等的影响.结果表明:土壤盐胁迫下,木麻黄幼苗根系活力、根系总长(TRL)、总根表面积(TRSA)、总根体积(TRV)、总根直径(TRD)、根长密度(RLD)、根表面积密度(SAD)降低,根组织密度(RTD)增加;接菌处理显著提高了根系活力和生物量,同一菌株处理下根系形态和养分特征受土壤盐度变化影响较大;与CK相比,接菌处理显著提高了TRD以及3‰和6‰盐度下根系活力、全磷(TP)含量和9‰盐度下TRV、TRSA和RSD.此外,3‰盐度下,Z1菌处理的TRL、RLD、根全氮(TN)含量显著高于CK,碳磷比(C/P)显著低于CK.Z1+Y6菌处理还显著提高了3‰和6‰盐度下TRSA、TRV、SAD和根碳磷比(C/P). 9‰盐度下,Z1和Z1+Y6菌处理下TP含量和根系活力同样显著高于CK.采用逐步回归建立了回归方程模型,发现RLD是影响苗高的主要因素,根系活力和TC含量是影响地径的主要因素;通径分析表明RTD、TRV、TP和TRD为影响生物量的主要因素,其中TRD的直接作用影响最大,为正向贡献.综上,盐胁迫下接菌处理能够影响幼苗根系形态和养分吸收以及生物量积累,耐盐性菌株可能是通过诱导宿主根系生长发育提高自身耐盐性,其中混合菌剂更有利于缓解盐害;结果可为深入开发木麻黄抗逆微生物制剂提供科学依据.(图3表3参57)

Keyword :

养分含量 养分含量 内生真菌 内生真菌 土壤盐度 土壤盐度 根系形态 根系形态 短枝木麻黄 短枝木麻黄 通径分析 通径分析

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GB/T 7714 李冠军 , 余雯静 , 温昕怡 et al. 盐胁迫下接种内生真菌对短枝木麻黄幼苗生长的影响 [J]. | 应用与环境生物学报 , 2024 , 30 (04) : 777-785 .
MLA 李冠军 et al. "盐胁迫下接种内生真菌对短枝木麻黄幼苗生长的影响" . | 应用与环境生物学报 30 . 04 (2024) : 777-785 .
APA 李冠军 , 余雯静 , 温昕怡 , 苏亲桂 , 吴承祯 , 苏军 et al. 盐胁迫下接种内生真菌对短枝木麻黄幼苗生长的影响 . | 应用与环境生物学报 , 2024 , 30 (04) , 777-785 .
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基于多组学量化松材线虫入侵对寄主马尾松的影响
期刊论文 | 2024 , 39 (06) , 720-729 | 福建农业学报
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Abstract :

【目的】探究松材线虫入侵对寄主马尾松的影响。【方法】以4年生马尾松(Pinus massoniana)为试材,以皮接法接入5 000条松材线虫的马尾松为处理组[PWN(+)组],以接入无菌水的马尾松为对照组[PWN(-)组],基于多组学(表型组学、转录组学、宏基因组学、代谢组学)量化松材线虫入侵马尾松14 d后的变化。【结果】与PWN(-)组相比,PWN(+)组活性氧(ROS)含量和H_2O_2含量分别显著上升3.2倍和1.7倍(P<0.05);编码应激反应通路的c60547.graph_c0、 c82953.graph_c0在PWN(+)组中的表达水平显著高于PWN(-)组(P<0.05),萜类生物合成途径通路的c64867.graph_c0、 c68789.graph_c0及合胞体形成通路的c81022.graph_c0在PWN(+)组中的表达水平显著低于PWN(-)组(P<0.05);PWN(+)组的微生物多样性显著低于PWN(-)组(P<0.05),肉座菌目(Hypocreales)是其体内的优势微生物,在生物信息数据库KEGG的功能注释和丰度信息主要集中在复制修复通路、DNA复制通路(PATH:ko03030)、DNA复制蛋白通路(BR:ko03032);代谢组上、下调的差异代谢物分别有365个和351个,PWN(+)组中根皮素、熊去氧胆酸、羧苄青霉素等物质的含量会增加以抵御松材线虫的侵染,差异显著的代谢物显著富集于ABC转运蛋白通路、花生四烯酸代谢通路、类黄酮生物合成通路、甘油磷脂代谢通路。【结论】当松材线虫入侵马尾松时,寄主会启动一系列复杂的防御反应。这些反应并非孤立存在,而是通过多种机制协同作用,共同应对松材线虫的侵染。上述结果有助于从多组学角度阐明松材线虫入侵对马尾松的影响,并为松材线虫病害诱导的森林衰退和寄主植物马尾松之间的相互作用提供依据。

Keyword :

代谢组 代谢组 宏基因组 宏基因组 松材线虫 松材线虫 活性氧 活性氧 转录组 转录组 马尾松 马尾松

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GB/T 7714 徐清华 , 郭芷晴 , 贾佳宇 et al. 基于多组学量化松材线虫入侵对寄主马尾松的影响 [J]. | 福建农业学报 , 2024 , 39 (06) : 720-729 .
MLA 徐清华 et al. "基于多组学量化松材线虫入侵对寄主马尾松的影响" . | 福建农业学报 39 . 06 (2024) : 720-729 .
APA 徐清华 , 郭芷晴 , 贾佳宇 , 苏军 . 基于多组学量化松材线虫入侵对寄主马尾松的影响 . | 福建农业学报 , 2024 , 39 (06) , 720-729 .
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