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学者姓名：赵新旺
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本发明公开了一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr03‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅3号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 邓祖湖 , 刘家睿 , 徐良年 et al. 一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法 : CN202510102827.1[P]. | 2025-01-22 . |
| MLA | 邓祖湖 et al. "一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法" : CN202510102827.1. | 2025-01-22 . |
| APA | 邓祖湖 , 刘家睿 , 徐良年 , 赵新旺 , 李雪停 , 黄国强 . 一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法 : CN202510102827.1. | 2025-01-22 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr01‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅1号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 邓祖湖 , 赵新旺 , 郭哲君 et al. 一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法 : CN202510102438.9[P]. | 2025-01-22 . |
| MLA | 邓祖湖 et al. "一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法" : CN202510102438.9. | 2025-01-22 . |
| APA | 邓祖湖 , 赵新旺 , 郭哲君 , 徐良年 , 李雪停 , 刘家睿 . 一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法 : CN202510102438.9. | 2025-01-22 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr04‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅4号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 徐良年 , 邓祖湖 , 郭哲君 et al. 一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法 : CN202510102879.9[P]. | 2025-01-22 . |
| MLA | 徐良年 et al. "一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法" : CN202510102879.9. | 2025-01-22 . |
| APA | 徐良年 , 邓祖湖 , 郭哲君 , 黄潮华 , 李雪停 , 赵新旺 . 一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法 : CN202510102879.9. | 2025-01-22 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr02‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅2号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 邓祖湖 , 徐良年 , 赵新旺 et al. 一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法 : CN202510103245.5[P]. | 2025-01-22 . |
| MLA | 邓祖湖 et al. "一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法" : CN202510103245.5. | 2025-01-22 . |
| APA | 邓祖湖 , 徐良年 , 赵新旺 , 黄国强 , 李雪停 , 黄潮华 . 一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法 : CN202510103245.5. | 2025-01-22 . |
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Sugarcane (Saccharum spp.), a vital economic crop, suffers significant yield losses from drought. This study elucidates the genetic regulation of lignin biosynthesis-a key drought-resistance mechanism-by analyzing three contrasting accessions: drought-sensitive Saccharum officinarum (Badila), drought-resistant hybrid (XTT22), and drought-tolerant wild Saccharum spontaneum (SES-208) under progressive drought (7-21 days). Physiological analyses revealed pronounced lignin accumulation in XTT22 roots/leaves, driven by elevated coniferyl/sinapyl alcohol substrates, while Badila showed minimal deposition. Genomic characterization of cinnamyl/sinapyl alcohol dehydrogenase (CAD/SAD) families across six sugarcane genomes identified 322 genes phylogenetically clustered into three clades. Class I members (CAD1, CAD5, etc.) were critical for lignin biosynthesis, with tandem/segmental duplications driving family expansion and promoters enriched in stress-responsive cis-elements (ABA, MeJA, light). Transcriptomics and qRT-PCR confirmed strong correlations between Class I CAD expression, lignin content, and drought tolerance. These findings establish CAD Class I genes as novel molecular targets for enhancing drought resilience in sugarcane breeding programs.
Keyword ：
CAD phylogenetic clades CAD phylogenetic clades drought resistance drought resistance lignin precursor regulation lignin precursor regulation molecular breeding molecular breeding Saccharum spp. Saccharum spp.
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| GB/T 7714 | Wang, Yue , Zhao, Weitong , Li, Peiting et al. Lignin Biosynthesis Driven by CAD Genes Underpins Drought Tolerance in Sugarcane: Genomic Insights for Crop Improvement [J]. | PLANTS-BASEL , 2025 , 14 (17) . |
| MLA | Wang, Yue et al. "Lignin Biosynthesis Driven by CAD Genes Underpins Drought Tolerance in Sugarcane: Genomic Insights for Crop Improvement" . | PLANTS-BASEL 14 . 17 (2025) . |
| APA | Wang, Yue , Zhao, Weitong , Li, Peiting , Zhao, Junjie , Yang, Zhiwei , Huang, Chaohua et al. Lignin Biosynthesis Driven by CAD Genes Underpins Drought Tolerance in Sugarcane: Genomic Insights for Crop Improvement . | PLANTS-BASEL , 2025 , 14 (17) . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr05‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅5号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 刘家睿 , 邓祖湖 , 赵新旺 et al. 一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法 : CN202510102916.6[P]. | 2025-01-22 . |
| MLA | 刘家睿 et al. "一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法" : CN202510102916.6. | 2025-01-22 . |
| APA | 刘家睿 , 邓祖湖 , 赵新旺 , 徐良年 , 李雪停 , 黄潮华 . 一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法 : CN202510102916.6. | 2025-01-22 . |
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Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol, so increasing its yield is essential to ensure the sugar security and bioenergy production. Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants, particularly those species with high ploidy such as sugarcane (Saccharum spp.). Tripidium arundinaceum exhibits many desirable agronomic traits, and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding. However, the genetic relationship between T. arundinaceum and Saccharum species, and the individual T. arundinaceum chromosomal compositions in sugarcane hybrids are still elusive. Here we used whole-genome single-nucleotide polymorphisms (SNPs) to ascertain the phylogenetic relationships between these species and found that T. arundinaceum is more closely related to Saccharum than Sorghum, in contrast to the previous narrow derived from Saccharum officinarum was able to distinctly identify the chromosomes of T. arundinaceum. We developed the oligo-genomic in situ hybridization (GISH) system for the first time, to unveil the novel chromosome translocations and the transmission of individual T. arundinaceum chromosomes in sugarcane progeny. Notably, we discovered that the chromosomal transmission of T. arundinaceum exhibited several different inheritance modes, including n, 2n, and over 2n in the BC1 1 progenies. Such inheritance patterns may have resulted from first division restitution (FDR) or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/ second division restitution (FDR+NSC/SDR) model during meiosis. These results will be of substantial benefit for the further selection of T. arundinaceum chromosomes for sugarcane genetic improvement.
Keyword ：
chromosomal transmission chromosomal transmission chromosome painting chromosome painting Oligo-FISH Oligo-FISH sugarcane sugarcane Tripidium arundinaceum Tripidium arundinaceum
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| GB/T 7714 | Yu, Fan , Yu, Zehuai , Chai, Jin et al. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny [J]. | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (11) : 3751-3762 . |
| MLA | Yu, Fan et al. "Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny" . | JOURNAL OF INTEGRATIVE AGRICULTURE 23 . 11 (2024) : 3751-3762 . |
| APA | Yu, Fan , Yu, Zehuai , Chai, Jin , Yu, Xikai , Fu, Chen , Zhao, Xinwang et al. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny . | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (11) , 3751-3762 . |
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The sucrose yield in sugarcane largely depends on stem morphology, including length, diameter and sugar content, making sugarcane stem a key trait in breeding. The "Bainianzhe" variety from Songxi County, Fujian Province, possesses both aerial stems and rhizomes, providing a unique model for studying stem development. We performed a spatiotemporal transcriptomic analysis of the base, middle and apical sections of both aerial stems and rhizomes. The analysis categorized transcriptomes by developmental stage-base, middle and apical-rather than environmental differences. Apical segments were enriched with genes related to cell proliferation, while base segments were linked to senescence and fibrosis. Gene regulatory networks revealed key TFs involved in stem development. Orphan genes may be involved in rhizome development through coexpression networks. Plant hormones, especially genes involved in ABA and GAs synthesis, were highly expressed in rhizomes. Thiamine-related genes were also more prevalent in rhizomes. Furthermore, the apical segments of rhizomes enriched in photosynthesis-related genes suggest adaptations to light exposure. Low average temperatures in Songxi have led to unique cold acclimation in Bainianzhe, with rhizomes showing higher expression of genes linked to unsaturated fatty acid synthesis and cold-responsive calcium signalling. This indicates that rhizomes may have enhanced cold tolerance, aiding in the plant's overwintering success.
Keyword ：
aerial stems aerial stems Bainianzhe Bainianzhe orphan genes orphan genes rhizomes rhizomes sugarcane sugarcane transcriptome transcriptome
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| GB/T 7714 | Li, Peiting , Yang, Ruiting , Liu, Jiarui et al. Coexpression Regulation of New and Ancient Genes in the Dynamic Transcriptome Landscape of Stem and Rhizome Development in "Bainianzhe"-An Ancient Chinese Sugarcane Variety Ratooned for Nearly 300 Years [J]. | PLANT CELL AND ENVIRONMENT , 2024 , 48 (2) : 1621-1642 . |
| MLA | Li, Peiting et al. "Coexpression Regulation of New and Ancient Genes in the Dynamic Transcriptome Landscape of Stem and Rhizome Development in "Bainianzhe"-An Ancient Chinese Sugarcane Variety Ratooned for Nearly 300 Years" . | PLANT CELL AND ENVIRONMENT 48 . 2 (2024) : 1621-1642 . |
| APA | Li, Peiting , Yang, Ruiting , Liu, Jiarui , Huang, Chaohua , Huang, Guoqiang , Deng, Zuhu et al. Coexpression Regulation of New and Ancient Genes in the Dynamic Transcriptome Landscape of Stem and Rhizome Development in "Bainianzhe"-An Ancient Chinese Sugarcane Variety Ratooned for Nearly 300 Years . | PLANT CELL AND ENVIRONMENT , 2024 , 48 (2) , 1621-1642 . |
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本发明公开了一套甘蔗品种真实性检测SNP标记及应用，包括177个核心SNP标记，SNP标记符合下述条件：F_MISSING≤0.3||MAF≥0.05，QUAL≥30.0||QD≥2.0||FS≤60.0||MQ≥40.0||SOR≤4.0条件；hwe平衡常数p≥0.01；均匀分布在甘蔗各染色体上且该位点上下100bp没有其他变异位点；采用LDR多重SNP分型试剂盒进行标记开发，能够转化成果的位点。本发明的优点：该套SNP标记组合建立DNA指纹图谱库，从而可以对甘蔗品种进行聚类分析和品种鉴定，使得检测结果更精准、高效；该套SNP标记稳定性好，均匀分布在甘蔗的80条染色体上，具有较好的可重复性；基于LDR技术的SNP引物组合在DNA质量满足常规PCR反应需求的情况下，对甘蔗品种检测的准确度和分辨率均很高，检测效率是SSR标记的10‑20倍，检测成本与SSR标记相当，检测过程更加快速、通量更高，同时检测过程中无需使用丙烯酰胺等有毒化学试剂，安全性高、环境友好。
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| GB/T 7714 | 赵新旺 , 赵薇彤 , 邓祖湖 et al. 一套甘蔗品种真实性检测SNP标记及应用 : CN202410647596.8[P]. | 2024-05-23 . |
| MLA | 赵新旺 et al. "一套甘蔗品种真实性检测SNP标记及应用" : CN202410647596.8. | 2024-05-23 . |
| APA | 赵新旺 , 赵薇彤 , 邓祖湖 , 张木清 , 徐良年 , 黄国强 et al. 一套甘蔗品种真实性检测SNP标记及应用 : CN202410647596.8. | 2024-05-23 . |
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Abstract ：
Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol,so increasing its yield is essential to ensure the sugar security and bioenergy production.Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants,particularly those species with high ploidy such as sugarcane(Saccharum spp.).Tripidium arundinaceum exhibits many desirable agronomic traits,and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding.However,the genetic relationship between T.arundinaceum and Saccharum species,and the individual T.arundinaceum chromosomal compositions in sugarcane hybrids are still elusive.Here we used whole-genome single-nucleotide polymorphisms(SNPs)to ascertain the phylogenetic relationships between these species and found that T.arundinaceum is more closely related to Saccharum than Sorghum,in contrast to the previous narrow genetic analyses using chloroplast DNA.Additionally,oligonucleotide(oligo)-based chromosome-specific painting derived from Saccharum officinarum was able to distinctly identify the chromosomes of T.arundinaceum.We developed the oligo-genomic in situ hybridization(GISH)system for the first time,to unveil the novel chromosome translocations and the transmission of individual T.arundinaceum chromosomes in sugarcane progeny.Notably,we discovered that the chromosomal transmission of T.arundinaceum exhibited several different inheritance modes,including n,2n,and over 2n in the BC1 progenies.Such inheritance patterns may have resulted from first division restitution(FDR)or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/second division restitution(FDR+NSC/SDR)model during meiosis.These results will be of substantial benefit for the further selection of T.arundinaceum chromosomes for sugarcane genetic improvement.
Cite：
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| GB/T 7714 | Fan Yu , Zehuai Yu , Jin Chai et al. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny [J]. | 农业科学学报（英文版） , 2024 , 23 (11) : 3751-3762 . |
| MLA | Fan Yu et al. "Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny" . | 农业科学学报（英文版） 23 . 11 (2024) : 3751-3762 . |
| APA | Fan Yu , Zehuai Yu , Jin Chai , Xikai Yu , Chen Fu , Xinwang Zhao et al. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny . | 农业科学学报（英文版） , 2024 , 23 (11) , 3751-3762 . |
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