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学者姓名：苏亚春
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Abstract ：
Plant chitinase is a pathogenesis-related protein that can hydrolyze chitin, the main component of fungal cell walls, and plays an important role in plant disease defense responses. Our previous study found the sugarcane class IV chitinase gene ScChiIV1 (GenBank Accession No. KP165001) was responding positively to smut pathogen Sporisorium scitamineum stress, but its disease resistance function and mechanism were unclear. Here, the upstream promoter of the ScChiIV1 gene (pro-ScChiIV1) with a length of 1696 bp was cloned which contained cis- acting elements related to hormone and stress response. Transient overexpression of the pro-ScChiIV1 in Nicotiana benthamiana showed inducible transcriptional levels by ABA, Fusarium solani var. coeruleum, and Alternaria longipes stimuli. Furthermore, stable overexpression of the ScChiIV1 gene in N. benthamiana enhanced the resistance of transgenic plants against F. solani var. coeruleum and S. scitamineum. Phenotypic monitoring, relevant physiological indicators, immune-related gene expression, and transcriptome analyses revealed that ScChiIV1 may activate potential TFs and PKs by inducing Ca2+ influx, ROS generation, and MAPK activation, thereby increasing the expression level of genes related to hormone signaling pathways, hypersensitive response (HR), and reactive oxygen species (ROS), as well as the activities of chitinase, superoxide dismutase (SOD), and catalase (CAT). In addition, ScChiIV1 reduced the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in transgenic plants, ultimately increasing disease resistance. This study provides novel insights into the molecular mechanism of the early response of the ScChiIV1 gene to pathogen stress and offers an excellent genetic resource for sugarcane disease resistant breeding.
Keyword ：
Chitinase Chitinase Disease resistance mechanism Disease resistance mechanism Expression analysis Expression analysis Fungal stress Fungal stress Sugarcane Sugarcane
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| GB/T 7714 | Chen, Yanling , Huang, Tingchen , You, Chuihuai et al. The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 221 . |
| MLA | Chen, Yanling et al. "The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 221 (2025) . |
| APA | Chen, Yanling , Huang, Tingchen , You, Chuihuai , Chen, Yao , Chen, Yan , Que, Youxiong et al. The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 221 . |
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Keyword ：
cell differentiation cell differentiation gene function gene function scRNA-seq scRNA-seq Sporisorium scitanmineum Sporisorium scitanmineum sugarcane sugarcane
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| GB/T 7714 | Zang, Shoujian , Wu, Qibin , Wang, Dongjiao et al. Cellular heterogeneity and immune responses to smut pathogen in sugarcane [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (7) : 2608-2610 . |
| MLA | Zang, Shoujian et al. "Cellular heterogeneity and immune responses to smut pathogen in sugarcane" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 7 (2025) : 2608-2610 . |
| APA | Zang, Shoujian , Wu, Qibin , Wang, Dongjiao , Li, Zhenxiang , Sun, Tingting , Sun, Xinlu et al. Cellular heterogeneity and immune responses to smut pathogen in sugarcane . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (7) , 2608-2610 . |
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Abstract ：
甘蔗(Saccharum spp.)是重要的糖料作物，病害、寒害、干旱等生物和非生物胁迫是限制甘蔗生产的重要环境因素。β-1,3-葡聚糖酶(β-1,3-glucanase, EC 3.2.1.39)作为病程相关蛋白家族的一员，不仅在植物对病原菌的防御中发挥重要作用，还参与调控植物的生长发育和非生物逆境应答。为了系统性研究甘蔗β-1,3-葡聚糖酶基因家族，本研究从甘蔗野生种割手密‘Np-X’、热带种‘LA-Purple’和栽培种‘R570’基因组中鉴定到132个糖苷水解酶(glycoside hydrolase, GH) 17家族成员。通过进化树分析，这些成员被划分为4个亚家族，其中亚家族Ⅳ占比最大(102个)。甘蔗GH17家族包含5个保守基序，内含子数量介于0–16之间，且多为全基因组复制类型(割手密‘Np-X’和热带种‘LA-Purple’占89.50%)，而栽培种‘R570’则以分散型复制为主(占58.10%)。启动子分析发现4类顺式作用元件，涉及植物生长发育及组织特异性表达(14.21%)、光响应(38.24%)、生物或非生物胁迫响应(9.18%)和激素响应(38.37%)，提示该基因家族参与植物的生长发育、激素响应和逆境应答。转录组和实时荧光定量PCR(quantitative real-time PCR, RT-qPCR)分析结果表明，甘蔗GH17基因存在组织特异性表达，且在低温、干旱、激素处理以及不同甘蔗基因型与黑穗病菌互作过程中差异表达，提示其在植物防御机制中有一定潜在作用，某些SsGlu基因(SsGlu5、SsGlu20、SsGlu21、SsGlu25、SsGlu28、SsGlu39)有望作为候选抗逆相关基因。本研究为进一步揭示甘蔗β-1,3-葡聚糖酶基因家族的抗逆分子机制奠定了基础。
Keyword ：
3-葡聚糖酶基因家族 3-葡聚糖酶基因家族 β-1 β-1 全基因组分析 全基因组分析 基因表达 基因表达 甘蔗 甘蔗 逆境胁迫 逆境胁迫
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| GB/T 7714 | 黄廷辰 , 夏逸飞 , 骆榆榕 et al. 甘蔗β-1,3-葡聚糖酶基因家族的鉴定及其在不同逆境胁迫下的表达分析 [J]. | 生物工程学报 , 2025 , 41 (07) : 2913-2933 . |
| MLA | 黄廷辰 et al. "甘蔗β-1,3-葡聚糖酶基因家族的鉴定及其在不同逆境胁迫下的表达分析" . | 生物工程学报 41 . 07 (2025) : 2913-2933 . |
| APA | 黄廷辰 , 夏逸飞 , 骆榆榕 , 臧守建 , 陈燕 , 刘清洪 et al. 甘蔗β-1,3-葡聚糖酶基因家族的鉴定及其在不同逆境胁迫下的表达分析 . | 生物工程学报 , 2025 , 41 (07) , 2913-2933 . |
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| GB/T 7714 | Wu, Qibin , Chen, Yanling , Zou, Wenhui et al. Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023) [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 295 . |
| MLA | Wu, Qibin et al. "Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023)" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 295 (2025) . |
| APA | Wu, Qibin , Chen, Yanling , Zou, Wenhui , Pan, Yong-Bao , Lin, Peixia , Xu, Liping et al. Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023) . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 295 . |
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Abstract ：
Sugarcane smut is a worldwide fungal disease caused by Sporisorium scitamineum, which seriously jeopardizes the healthy and sustainable development of the sugarcane industry. WRKY transcription factors are actively involved in plant responses to pathogen infection; however, the mechanisms by which WRKY mediates resistance to sugarcane smut remain elusive. Here, we obtained a nucleus-localized transcription factor ScWRKY2, which was down-regulated in smut-resistant but up-regulated in smut-susceptible sugarcane cultivars. Overexpression of the ScWRKY2 gene in sugarcane enhanced its sensitivity to smut disease. Besides, an immune-related gene ScLRR-RLK, directly regulated by ScWRKY2, was identified through a combination of transcriptome and DNA affinity purification sequencing. Interestingly, ScWRKY2 suppressed the expression of the ScLRR-RLK gene by binding to the W-box element in its promoter region. Furthermore, protein-protein interaction assays demonstrated that ScWRKY2 interacted with the ScPsbP protein in the nucleus. Notably, co-overexpression of ScWRKY2 and ScPsbP enhanced the sensitivity of plants to pathogens by inducing the expression of reactive oxygen species (ROS) scavenging-related genes. Collectively, our study revealed that ScWRKY2 negatively regulated sugarcane resistance to smut disease by repressing the expression of the ScLRR-RLK gene, followed by interacting with the chloroplast protein ScPsbP, and thus inducing the expression of ROS scavenging-related genes to maintain ROS homeostasis. These findings provided a theoretical basis and gene resources for sugarcane disease resistance breeding.
Keyword ：
disease resistance function disease resistance function molecular mechanism molecular mechanism smut disease smut disease sugarcane sugarcane WRKY WRKY
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| GB/T 7714 | Wang, Dongjiao , Gou, Yaxin , Yi, Chao et al. ScWRKY2: a key regulator for smut resistance in sugarcane [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (9) : 3667-3681 . |
| MLA | Wang, Dongjiao et al. "ScWRKY2: a key regulator for smut resistance in sugarcane" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 9 (2025) : 3667-3681 . |
| APA | Wang, Dongjiao , Gou, Yaxin , Yi, Chao , Li, Zhenxiang , Wang, Wenzhi , Lin, Peixia et al. ScWRKY2: a key regulator for smut resistance in sugarcane . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (9) , 3667-3681 . |
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Keyword ：
immune homeostasis immune homeostasis pathogenesis-related gene pathogenesis-related gene sugarcane sugarcane WRKY transcription factor WRKY transcription factor
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| GB/T 7714 | Zang, Shoujian , Wang, Dongjiao , Qin, Liqian et al. ScWRKY6 Interacts With ScSAG39 to Regulate Immune Homeostasis by Transcriptional Control of ScPR1 [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 . |
| MLA | Zang, Shoujian et al. "ScWRKY6 Interacts With ScSAG39 to Regulate Immune Homeostasis by Transcriptional Control of ScPR1" . | PLANT BIOTECHNOLOGY JOURNAL (2025) . |
| APA | Zang, Shoujian , Wang, Dongjiao , Qin, Liqian , Cui, Shijiang , Wu, Guran , Liu, Kaisheng et al. ScWRKY6 Interacts With ScSAG39 to Regulate Immune Homeostasis by Transcriptional Control of ScPR1 . | PLANT BIOTECHNOLOGY JOURNAL , 2025 . |
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本发明提供一种具有抗病功能的ScPDC1蛋白及其编码基因和应用，属于生物技术领域，并具体公开了甘蔗的ScPDC1基因在提高抗病能力中的应用。本发明通过对甘蔗中PLN02573超家族的ScPDC1基因进行研究，有助于了解甘蔗抗病机理，并为利用基因工程技术等诱导植物具有抗病功能，提供了理论依据和参考。
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| GB/T 7714 | 吴期滨 , 阙友雄 , 封翱崟 et al. 一种具有抗病功能的ScPDC1蛋白及其编码基因和应用 : CN202411062033.9[P]. | 2024-08-05 . |
| MLA | 吴期滨 et al. "一种具有抗病功能的ScPDC1蛋白及其编码基因和应用" : CN202411062033.9. | 2024-08-05 . |
| APA | 吴期滨 , 阙友雄 , 封翱崟 , 苏亚春 , 孙婷婷 , 赵婉莹 . 一种具有抗病功能的ScPDC1蛋白及其编码基因和应用 : CN202411062033.9. | 2024-08-05 . |
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本发明属于生物技术领域，涉及一种快速培育转基因甘蔗的方法，尤其涉及一种利用甘蔗种茎快速获得转基因甘蔗的方法。针对现有甘蔗遗传转化周期时间久、组织培养工作量大的问题，直接利用甘蔗种茎，在超声波的作用下，通过农杆菌介导法进行甘蔗遗传转化，可快速高效地获得转基因甘蔗，比一般的农杆菌介导甘蔗遗传方法对比，转化时间可缩短50%以上，工作量减少80%以上。
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| GB/T 7714 | 许莉萍 , 杨颖颖 , 高世武 et al. 一种利用甘蔗种茎快速获得转基因甘蔗的方法 : CN202410054808.1[P]. | 2024-01-15 . |
| MLA | 许莉萍 et al. "一种利用甘蔗种茎快速获得转基因甘蔗的方法" : CN202410054808.1. | 2024-01-15 . |
| APA | 许莉萍 , 杨颖颖 , 高世武 , 郭晋隆 , 阙友雄 , 苏亚春 et al. 一种利用甘蔗种茎快速获得转基因甘蔗的方法 : CN202410054808.1. | 2024-01-15 . |
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12-Oxo-phytodienoic acid reductases (OPRs) perform vital functions in plants. However, few studies have been reported in sugarcane (Saccharum spp.), and it is of great significance to systematically investigates it in sugarcane. Here, 61 ShOPRs, 32 SsOPRs, and 36 SoOPRs were identified from R570 (Saccharum spp. hybrid cultivar R570), AP85-441 (Saccharum spontaneum), and LA-purple (Saccharum officinarum), respectively. These OPRs were phylogenetically classified into four groups, with close genes similar structures. During evolution, OPR gene family was mainly expanded via whole-genome duplications/segmental events and predominantly underwent purifying selection, while sugarcane OPR genes may function differently in response to various stresses. Further, ScOPR2, a tissue-specific OPR, which was localized in cytoplasm and cell membrane and actively response to salicylic acid (SA), methyl jasmonate, and smut pathogen (Sporisorium scitamineum) stresses, was cloned from sugarcane. In addition, both its transient overexpression and stable overexpression enhanced the resistance of transgenic plants to pathogen infection, most probably through activating pathogen-associated molecular pattern/pattern-recognition receptor-triggered immunity, producing reactive oxygen species, and initiating mitogen-activated protein kinase cascade. Subsequently, the transmission of SA and hypersensitive reaction were triggered, which stimulated the transcription of defense-related genes. These findings provide insights into the function of ScOPR2 gene for disease resistance.
Keyword ：
12-oxo-phytodienoic acid reductases 12-oxo-phytodienoic acid reductases disease resistance disease resistance genome-wide identification genome-wide identification RNA-seq RNA-seq sugarcane sugarcane
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| GB/T 7714 | Sun, Tingting , Wu, Qibin , Zang, Shoujian et al. Molecular insights into OPR gene family in Saccharum identified a ScOPR2 gene could enhance plant disease resistance [J]. | PLANT JOURNAL , 2024 , 120 (1) : 335-353 . |
| MLA | Sun, Tingting et al. "Molecular insights into OPR gene family in Saccharum identified a ScOPR2 gene could enhance plant disease resistance" . | PLANT JOURNAL 120 . 1 (2024) : 335-353 . |
| APA | Sun, Tingting , Wu, Qibin , Zang, Shoujian , Zou, Wenhui , Wang, Dongjiao , Wang, Wenzhi et al. Molecular insights into OPR gene family in Saccharum identified a ScOPR2 gene could enhance plant disease resistance . | PLANT JOURNAL , 2024 , 120 (1) , 335-353 . |
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12-Oxo-phytodienoate reductase (OPR) is an oxidoreductase that depends on flavin mononucleotide (FMN) and catalyzes the conversion of 12-oxophytodienoate (12-OPDA) into jasmonic acid (JA). It plays a key role in plant growth and development, and resistance to adverse stresses. In our previous study, we have obtained an OPR gene (ScOPR1, GenBank Accession Number: MG755745) from sugarcane. This gene showed positive responses to methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), and Sporisorium scitamineum, suggesting its potential for pathogen resistance. Here, in our study, we observed that Nicotiana benthamiana leaves transiently overexpressing ScOPR1 exhibited weaker disease symptoms, darker 3,3-diaminobenzidine (DAB) staining, higher accumulation of reactive oxygen species (ROS), and higher expression of hypersensitive response (HR) and SA pathway-related genes after inoculation with Ralstonia solanacearum and Fusarium solanacearum var. coeruleum. Furthermore, the transgenic N. benthamiana plants stably overexpressing the ScOPR1 gene showed enhanced resistance to pathogen infection by increasing the accumulation of JA, SA, and glutathione S-transferase (GST), as well as up-regulating genes related to HR, JA, SA, and ROS signaling pathways. Transcriptome analysis revealed that the specific differentially expressed genes (DEGs) in ScOPR1-OE were significantly enriched in hormone transduction signaling and plant-pathogen interaction pathways. Finally, a functional mechanism model of the ScOPR1 gene in response to pathogen infection was depicted. This study provides insights into the molecular mechanism of ScOPR1 and presents compelling evidence supporting its positive involvement in enhancing plant disease resistance.
Keyword ：
12-Oxo-phytodienoic acid reductase 12-Oxo-phytodienoic acid reductase Genetic transformation Genetic transformation Pathogen infection Pathogen infection Resistance mechanism Resistance mechanism Sugarcane Sugarcane
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| GB/T 7714 | Zou, Wenhui , Sun, Tingting , Chen, Yao et al. Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways [J]. | PLANT CELL REPORTS , 2024 , 43 (6) . |
| MLA | Zou, Wenhui et al. "Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways" . | PLANT CELL REPORTS 43 . 6 (2024) . |
| APA | Zou, Wenhui , Sun, Tingting , Chen, Yao , Wang, Dongjiao , You, Chuihuai , Zang, Shoujian et al. Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways . | PLANT CELL REPORTS , 2024 , 43 (6) . |
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