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学者姓名：苏亚春
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Abstract ：
Plant chitinase is a pathogenesis-related protein that can hydrolyze chitin, the main component of fungal cell walls, and plays an important role in plant disease defense responses. Our previous study found the sugarcane class IV chitinase gene ScChiIV1 (GenBank Accession No. KP165001) was responding positively to smut pathogen Sporisorium scitamineum stress, but its disease resistance function and mechanism were unclear. Here, the upstream promoter of the ScChiIV1 gene (pro-ScChiIV1) with a length of 1696 bp was cloned which contained cis- acting elements related to hormone and stress response. Transient overexpression of the pro-ScChiIV1 in Nicotiana benthamiana showed inducible transcriptional levels by ABA, Fusarium solani var. coeruleum, and Alternaria longipes stimuli. Furthermore, stable overexpression of the ScChiIV1 gene in N. benthamiana enhanced the resistance of transgenic plants against F. solani var. coeruleum and S. scitamineum. Phenotypic monitoring, relevant physiological indicators, immune-related gene expression, and transcriptome analyses revealed that ScChiIV1 may activate potential TFs and PKs by inducing Ca2+ influx, ROS generation, and MAPK activation, thereby increasing the expression level of genes related to hormone signaling pathways, hypersensitive response (HR), and reactive oxygen species (ROS), as well as the activities of chitinase, superoxide dismutase (SOD), and catalase (CAT). In addition, ScChiIV1 reduced the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in transgenic plants, ultimately increasing disease resistance. This study provides novel insights into the molecular mechanism of the early response of the ScChiIV1 gene to pathogen stress and offers an excellent genetic resource for sugarcane disease resistant breeding.
Keyword ：
Chitinase Chitinase Disease resistance mechanism Disease resistance mechanism Expression analysis Expression analysis Fungal stress Fungal stress Sugarcane Sugarcane
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| GB/T 7714 | Chen, Yanling , Huang, Tingchen , You, Chuihuai et al. The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 221 . |
| MLA | Chen, Yanling et al. "The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 221 (2025) . |
| APA | Chen, Yanling , Huang, Tingchen , You, Chuihuai , Chen, Yao , Chen, Yan , Que, Youxiong et al. The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 221 . |
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Abstract ：
甘蔗(Saccharum spp.)是重要的糖料作物，病害、寒害、干旱等生物和非生物胁迫是限制甘蔗生产的重要环境因素。β-1,3-葡聚糖酶(β-1,3-glucanase, EC 3.2.1.39)作为病程相关蛋白家族的一员，不仅在植物对病原菌的防御中发挥重要作用，还参与调控植物的生长发育和非生物逆境应答。为了系统性研究甘蔗β-1,3-葡聚糖酶基因家族，本研究从甘蔗野生种割手密‘Np-X’、热带种‘LA-Purple’和栽培种‘R570’基因组中鉴定到132个糖苷水解酶(glycoside hydrolase, GH) 17家族成员。通过进化树分析，这些成员被划分为4个亚家族，其中亚家族Ⅳ占比最大(102个)。甘蔗GH17家族包含5个保守基序，内含子数量介于0–16之间，且多为全基因组复制类型(割手密‘Np-X’和热带种‘LA-Purple’占89.50%)，而栽培种‘R570’则以分散型复制为主(占58.10%)。启动子分析发现4类顺式作用元件，涉及植物生长发育及组织特异性表达(14.21%)、光响应(38.24%)、生物或非生物胁迫响应(9.18%)和激素响应(38.37%)，提示该基因家族参与植物的生长发育、激素响应和逆境应答。转录组和实时荧光定量PCR(quantitative real-time PCR, RT-qPCR)分析结果表明，甘蔗GH17基因存在组织特异性表达，且在低温、干旱、激素处理以及不同甘蔗基因型与黑穗病菌互作过程中差异表达，提示其在植物防御机制中有一定潜在作用，某些SsGlu基因(SsGlu5、SsGlu20、SsGlu21、SsGlu25、SsGlu28、SsGlu39)有望作为候选抗逆相关基因。本研究为进一步揭示甘蔗β-1,3-葡聚糖酶基因家族的抗逆分子机制奠定了基础。
Keyword ：
3-葡聚糖酶基因家族 3-葡聚糖酶基因家族 β-1 β-1 全基因组分析 全基因组分析 基因表达 基因表达 甘蔗 甘蔗 逆境胁迫 逆境胁迫
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| GB/T 7714 | 黄廷辰 , 夏逸飞 , 骆榆榕 et al. 甘蔗β-1,3-葡聚糖酶基因家族的鉴定及其在不同逆境胁迫下的表达分析 [J]. | 生物工程学报 , 2025 , 41 (07) : 2913-2933 . |
| MLA | 黄廷辰 et al. "甘蔗β-1,3-葡聚糖酶基因家族的鉴定及其在不同逆境胁迫下的表达分析" . | 生物工程学报 41 . 07 (2025) : 2913-2933 . |
| APA | 黄廷辰 , 夏逸飞 , 骆榆榕 , 臧守建 , 陈燕 , 刘清洪 et al. 甘蔗β-1,3-葡聚糖酶基因家族的鉴定及其在不同逆境胁迫下的表达分析 . | 生物工程学报 , 2025 , 41 (07) , 2913-2933 . |
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Keyword ：
cell differentiation cell differentiation gene function gene function scRNA-seq scRNA-seq Sporisorium scitanmineum Sporisorium scitanmineum sugarcane sugarcane
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| GB/T 7714 | Zang, Shoujian , Wu, Qibin , Wang, Dongjiao et al. Cellular heterogeneity and immune responses to smut pathogen in sugarcane [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (7) : 2608-2610 . |
| MLA | Zang, Shoujian et al. "Cellular heterogeneity and immune responses to smut pathogen in sugarcane" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 7 (2025) : 2608-2610 . |
| APA | Zang, Shoujian , Wu, Qibin , Wang, Dongjiao , Li, Zhenxiang , Sun, Tingting , Sun, Xinlu et al. Cellular heterogeneity and immune responses to smut pathogen in sugarcane . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (7) , 2608-2610 . |
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Abstract ：
Sugarcane smut is a worldwide fungal disease caused by Sporisorium scitamineum, which seriously jeopardizes the healthy and sustainable development of the sugarcane industry. WRKY transcription factors are actively involved in plant responses to pathogen infection; however, the mechanisms by which WRKY mediates resistance to sugarcane smut remain elusive. Here, we obtained a nucleus-localized transcription factor ScWRKY2, which was down-regulated in smut-resistant but up-regulated in smut-susceptible sugarcane cultivars. Overexpression of the ScWRKY2 gene in sugarcane enhanced its sensitivity to smut disease. Besides, an immune-related gene ScLRR-RLK, directly regulated by ScWRKY2, was identified through a combination of transcriptome and DNA affinity purification sequencing. Interestingly, ScWRKY2 suppressed the expression of the ScLRR-RLK gene by binding to the W-box element in its promoter region. Furthermore, protein-protein interaction assays demonstrated that ScWRKY2 interacted with the ScPsbP protein in the nucleus. Notably, co-overexpression of ScWRKY2 and ScPsbP enhanced the sensitivity of plants to pathogens by inducing the expression of reactive oxygen species (ROS) scavenging-related genes. Collectively, our study revealed that ScWRKY2 negatively regulated sugarcane resistance to smut disease by repressing the expression of the ScLRR-RLK gene, followed by interacting with the chloroplast protein ScPsbP, and thus inducing the expression of ROS scavenging-related genes to maintain ROS homeostasis. These findings provided a theoretical basis and gene resources for sugarcane disease resistance breeding.
Keyword ：
disease resistance function disease resistance function molecular mechanism molecular mechanism smut disease smut disease sugarcane sugarcane WRKY WRKY
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| GB/T 7714 | Wang, Dongjiao , Gou, Yaxin , Yi, Chao et al. ScWRKY2: a key regulator for smut resistance in sugarcane [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (9) : 3667-3681 . |
| MLA | Wang, Dongjiao et al. "ScWRKY2: a key regulator for smut resistance in sugarcane" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 9 (2025) : 3667-3681 . |
| APA | Wang, Dongjiao , Gou, Yaxin , Yi, Chao , Li, Zhenxiang , Wang, Wenzhi , Lin, Peixia et al. ScWRKY2: a key regulator for smut resistance in sugarcane . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (9) , 3667-3681 . |
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| GB/T 7714 | Wu, Qibin , Chen, Yanling , Zou, Wenhui et al. Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023) [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 295 . |
| MLA | Wu, Qibin et al. "Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023)" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 295 (2025) . |
| APA | Wu, Qibin , Chen, Yanling , Zou, Wenhui , Pan, Yong-Bao , Lin, Peixia , Xu, Liping et al. Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023) . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 295 . |
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Keyword ：
immune homeostasis immune homeostasis pathogenesis-related gene pathogenesis-related gene sugarcane sugarcane WRKY transcription factor WRKY transcription factor
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| GB/T 7714 | Zang, Shoujian , Wang, Dongjiao , Qin, Liqian et al. ScWRKY6 Interacts With ScSAG39 to Regulate Immune Homeostasis by Transcriptional Control of ScPR1 [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 . |
| MLA | Zang, Shoujian et al. "ScWRKY6 Interacts With ScSAG39 to Regulate Immune Homeostasis by Transcriptional Control of ScPR1" . | PLANT BIOTECHNOLOGY JOURNAL (2025) . |
| APA | Zang, Shoujian , Wang, Dongjiao , Qin, Liqian , Cui, Shijiang , Wu, Guran , Liu, Kaisheng et al. ScWRKY6 Interacts With ScSAG39 to Regulate Immune Homeostasis by Transcriptional Control of ScPR1 . | PLANT BIOTECHNOLOGY JOURNAL , 2025 . |
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12-Oxo-phytodienoate reductase (OPR) is an oxidoreductase that depends on flavin mononucleotide (FMN) and catalyzes the conversion of 12-oxophytodienoate (12-OPDA) into jasmonic acid (JA). It plays a key role in plant growth and development, and resistance to adverse stresses. In our previous study, we have obtained an OPR gene (ScOPR1, GenBank Accession Number: MG755745) from sugarcane. This gene showed positive responses to methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), and Sporisorium scitamineum, suggesting its potential for pathogen resistance. Here, in our study, we observed that Nicotiana benthamiana leaves transiently overexpressing ScOPR1 exhibited weaker disease symptoms, darker 3,3-diaminobenzidine (DAB) staining, higher accumulation of reactive oxygen species (ROS), and higher expression of hypersensitive response (HR) and SA pathway-related genes after inoculation with Ralstonia solanacearum and Fusarium solanacearum var. coeruleum. Furthermore, the transgenic N. benthamiana plants stably overexpressing the ScOPR1 gene showed enhanced resistance to pathogen infection by increasing the accumulation of JA, SA, and glutathione S-transferase (GST), as well as up-regulating genes related to HR, JA, SA, and ROS signaling pathways. Transcriptome analysis revealed that the specific differentially expressed genes (DEGs) in ScOPR1-OE were significantly enriched in hormone transduction signaling and plant-pathogen interaction pathways. Finally, a functional mechanism model of the ScOPR1 gene in response to pathogen infection was depicted. This study provides insights into the molecular mechanism of ScOPR1 and presents compelling evidence supporting its positive involvement in enhancing plant disease resistance.
Keyword ：
12-Oxo-phytodienoic acid reductase 12-Oxo-phytodienoic acid reductase Genetic transformation Genetic transformation Pathogen infection Pathogen infection Resistance mechanism Resistance mechanism Sugarcane Sugarcane
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| GB/T 7714 | Zou, Wenhui , Sun, Tingting , Chen, Yao et al. Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways [J]. | PLANT CELL REPORTS , 2024 , 43 (6) . |
| MLA | Zou, Wenhui et al. "Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways" . | PLANT CELL REPORTS 43 . 6 (2024) . |
| APA | Zou, Wenhui , Sun, Tingting , Chen, Yao , Wang, Dongjiao , You, Chuihuai , Zang, Shoujian et al. Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways . | PLANT CELL REPORTS , 2024 , 43 (6) . |
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WRKY transcription factors, transcriptional regulators unique to plants, play an important role in defense response to pathogen infection. However, the resistance mechanisms of WRKY genes in sugarcane remain unclear. In the present study, gene ontology (GO) enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense response. We identified gene ScWRKY4, a class IIc member of the WRKY gene family, in sugarcane cultivar ROC22. This gene was induced by salicylic acid (SA) and methyl jasmonate (MeJA) stress. Interestingly, expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium scitamineum. Moreover, stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani var. coeruleum and caused down-regulated expression of immune marker-related genes. Transcriptome analysis indicated suppressed expression of most JAZ genes in the signal transduction pathway. ScWRKY4 interacted with ScJAZ13 to repress its expression. We thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance, most likely through the JA signaling pathway. The present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC
Keyword ：
Disease resistance Disease resistance Expression profile Expression profile Transcriptome analysis Transcriptome analysis WRKY transcription factors WRKY transcription factors
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| GB/T 7714 | Wang, Dongjiao , Wang, Wei , Zang, Shoujian et al. Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway [J]. | CROP JOURNAL , 2024 , 12 (1) : 164-176 . |
| MLA | Wang, Dongjiao et al. "Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway" . | CROP JOURNAL 12 . 1 (2024) : 164-176 . |
| APA | Wang, Dongjiao , Wang, Wei , Zang, Shoujian , Qin, Liqian , Liang, Yanlan , Lin, Peixia et al. Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway . | CROP JOURNAL , 2024 , 12 (1) , 164-176 . |
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BackgroundGelsemium elegans is a traditional Chinese medicinal plant and temperature is one of the key factors affecting its growth. RAV (related to ABI3/VP1) transcription factor plays multiple roles in higher plants, including the regulation of plant growth, development, and stress response. However, RAV transcription factor in G. elegans has not been reported.ResultsIn this study, three novel GeRAV genes (GeRAV1-GeRAV3) were identified from the transcriptome of G. elegans under low temperature stress. Phylogenetic analysis showed that GeRAV1-GeRAV3 proteins were clustered into groups II, IV, and V, respectively. RNA-sequencing (RNA-seq) and real-time quantitative PCR (qRT-PCR) analyses indicated that the expression of GeRAV1 and GeRAV2 was increased in response to cold stress. Furthermore, the GeRAV1 gene was successfully cloned from G. elegans leaf. It encoded a hydrophilic, unstable, and non-secretory protein that contained both AP2 and B3 domains. The amino acid sequence of GeRAV1 protein shared a high similarity of 81.97% with Camptotheca acuminata CaRAV. Subcellular localization and transcriptional self-activation experiments demonstrated that GeRAV1 was a nucleoprotein without self-activating activity. The GeRAV1 gene was constitutively expressed in the leaves, stems, and roots of the G. elegans, with the highest expression levels in roots. In addition, the expression of the GeRAV1 gene was rapidly up-regulated under abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA) stresses, suggesting that it may be involved in hormonal signaling pathways. Moreover, GeRAV1 conferred improved cold and sodium chloride tolerance in Escherichia coli Rosetta cells.ConclusionsThese findings provided a foundation for further understanding on the function and regulatory mechanism of the GeRAV1 gene in response to low-temperature stress in G. elegans.
Keyword ：
Cold tolerance Cold tolerance Expression analysis Expression analysis Gelsemium elegans Gelsemium elegans Prokaryotic expression Prokaryotic expression RAV transcription factor RAV transcription factor
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| GB/T 7714 | Cui, Tianzhen , Zang, Shoujian , Sun, Xinlu et al. Molecular identification and functional characterization of a transcription factor GeRAV1 from Gelsemium elegans [J]. | BMC GENOMICS , 2024 , 25 (1) . |
| MLA | Cui, Tianzhen et al. "Molecular identification and functional characterization of a transcription factor GeRAV1 from Gelsemium elegans" . | BMC GENOMICS 25 . 1 (2024) . |
| APA | Cui, Tianzhen , Zang, Shoujian , Sun, Xinlu , Zhang, Jing , Su, Yachun , Wang, Dongjiao et al. Molecular identification and functional characterization of a transcription factor GeRAV1 from Gelsemium elegans . | BMC GENOMICS , 2024 , 25 (1) . |
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Calcium (Ca2+) is a second messenger in various physiological processes within plants. The significance of the Ca2+/H+ exchanger (CAX) has been established in facilitating Ca2+ transport in plants; however, disease resistance functions of the CAX gene remain elusive. In this study, we conducted sequence characterization and expression analysis for a sugarcane CAX gene, ScCAX4 (GenBank Accession Number: MW206380). In order to further investigate the disease resistance functions, this gene was then transiently overexpressed in Nicotiana benthamiana leaves, which were subsequently inoculated with Fusarium solani var. coeruleum. Results showed that ScCAX4 overexpression increased the susceptibility of N. benthamiana to pathogen infection by regulating the expression of genes related to salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) pathways, suggesting its negative role in disease resistance. Furthermore, we genetically transformed the ScCAX4 gene into N. benthamiana and obtained three positive T-2 generation lines. Interestingly, the symptomatology of transgenic plants was consistent with that of transient overexpression after pathogen inoculation. Notably, the JA content in transgenic overexpression lines was significantly higher than that in the wild-type. RNA-seq revealed that ScCAX4 could mediate multiple signaling pathways, and the JA signaling pathway played a key role in modulating disease resistance. Finally, a regulatory model was depicted for the increased susceptibility to pathogen infection conferred by the ScCAX4 gene. This study provides genetic resources for sugarcane molecular breeding and the research direction for plant CAX genes.
Keyword ：
CAX CAX disease resistance disease resistance regulatory mechanism regulatory mechanism RNA-seq RNA-seq sugarcane sugarcane
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| GB/T 7714 | Zhang, Chang , Li, Zhenxiang , Sun, Tingting et al. Sugarcane ScCAX4 is a Negative Regulator of Resistance to Pathogen Infection [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (23) : 13205-13216 . |
| MLA | Zhang, Chang et al. "Sugarcane ScCAX4 is a Negative Regulator of Resistance to Pathogen Infection" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 23 (2024) : 13205-13216 . |
| APA | Zhang, Chang , Li, Zhenxiang , Sun, Tingting , Zang, Shoujian , Wang, Dongjiao , Su, Yachun et al. Sugarcane ScCAX4 is a Negative Regulator of Resistance to Pathogen Infection . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (23) , 13205-13216 . |
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