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学者姓名：许莉萍
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摘要 ：
Sugarcane is the most important crop for sugar production. Sugarcane streak mosaic virus (SCSMV) triggered sugarcane mosaic disease can lead to substantial reductions in both yield and sucrose content. In the process of disease prevention and control, target pathogen detection technology is indispensable. However, traditional detection methods are time-consuming and require expensive equipment, making them less efficient for timely disease control and unfavorable to disease resistance breeding. Here, we introduce a novel detection technology that combines recombinase polymerase amplification (RPA) with CRISPR-Cas12a. The method utilizes crude extracts from sugarcane leaves as the reaction template, significantly simplifying and expediting the preparation process. By combining RPA and CRISPR-Cas12a in a single reaction tube, the risk of aerosol contamination has decreased markedly. The entire process, from sample preparation to result interpretation, only takes 50 min, and the reaction equipment only a water bath pot, and results can be blue light spectrometer or UV flashlight assessed visually. Importantly, the method demonstrates high sensitivity, detecting a minimum of 50 copies of the plasmid, which surpasses the sensitivity of reverse transcription polymerase chain reaction (RT-PCR) and is comparable to quantitative RT-PCR (RT-qPCR). The method exhibits excellent specificity, showing no cross- reactivity with other common sugarcane viruses, including Sugarcane mosaic virus, Sugarcane yellow leaf virus, and Sorghum mosaic virus. The practicality of this technique was validated through the detection of leaf crude extracts from 40 field samples. The detection results were consistent with those obtained from RT-PCR and RTqPCR using leaf RNA as the template, indicating its suitability for laboratory detection and field applications.
關鍵字 ：
CRISPR-Cas12a CRISPR-Cas12a Leaf crude extracts Leaf crude extracts RPA RPA Sugarcane Sugarcane Sugarcane streak mosaic virus Sugarcane streak mosaic virus
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| GB/T 7714 | Gao, Shuai , Guo, Jinlong , Wang, Ting et al. A rapid visual detection method for Sugarcane streak mosaic virus based on one-tube RPA-CRISPR/Cas12a [J]. | TALANTA , 2025 , 291 . |
| MLA | Gao, Shuai et al. "A rapid visual detection method for Sugarcane streak mosaic virus based on one-tube RPA-CRISPR/Cas12a" . | TALANTA 291 (2025) . |
| APA | Gao, Shuai , Guo, Jinlong , Wang, Ting , Xu, Liping . A rapid visual detection method for Sugarcane streak mosaic virus based on one-tube RPA-CRISPR/Cas12a . | TALANTA , 2025 , 291 . |
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摘要 ：
Sugarcane (Saccharum spp.) is globally considered an important crop for sugar and biofuel production. During sugarcane production, the heavy reliance on chemical nitrogen fertilizer has resulted in low nitrogen use efficiency (NUE) and high loss. Up until now, there has been extensive research on the transcriptomic dynamics during sugarcane response to low nitrogen (LN) stress. However, the specific contribution of S. spontaneum to the NUE of modern sugarcane remains unclear. In the present study, the comparative transcriptome analysis of two contrasting sugarcane cultivars in response to nitrogen deficiency was performed via the combination of genomes of S. spontaneum and S. officinarum. Sub-genome analysis indicated that S. spontaneum supports the high NUE of modern sugarcane by providing genes related to nitrogen and carbohydrate metabolism, photosynthesis, and amino acid metabolism. Additionally, the key genes involved in nitrogen metabolism from the S. spontaneum were successfully identified through weighted gene co-expression network analyses (WGCNA), and a high-affinity nitrate transporter named ScNRT2.3 was subsequently cloned. Heterogeneous expression of the ScNRT2.3, a cell membrane-localized protein, could enhance the growth of Arabidopsis under low nitrate conditions. Furthermore, a conserved protein module known as NAR2.1/NRT2.3 was shown to regulate the response to LN stress in sugarcane roots through molecular interaction. This work helps to clarify the contribution of S. spontaneum to the NUE in modern sugarcane, and the function of the ScNRT2.3 in sugarcane.
關鍵字 ：
genetic mechanisms genetic mechanisms nitrogen use efficiency nitrogen use efficiency ScNRT2.3 ScNRT2.3 sub-genome sub-genome sugarcane sugarcane
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| GB/T 7714 | Hui, Qianlong , Song, Ting , Yang, Dantong et al. Identification and Characterization of Key Genes for Nitrogen Utilization from Saccharum spontaneum Sub-Genome in Modern Sugarcane Cultivar [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (1) . |
| MLA | Hui, Qianlong et al. "Identification and Characterization of Key Genes for Nitrogen Utilization from Saccharum spontaneum Sub-Genome in Modern Sugarcane Cultivar" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 1 (2025) . |
| APA | Hui, Qianlong , Song, Ting , Yang, Dantong , Wu, Qibin , Guo, Jinlong , Que, Youxiong et al. Identification and Characterization of Key Genes for Nitrogen Utilization from Saccharum spontaneum Sub-Genome in Modern Sugarcane Cultivar . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (1) . |
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摘要 ：
Sugarcane is a tropical and subtropica cultivated crop that exhibits complex genetic traits. Key agronomic characteristics, such as tillering rate (T-rate) and the number of canes per hectare (NCPH), are crucial for yield optimization. This study employed a two-way pseudo-testcross strategy mapping approach with a 172 F1 population derived from 'Yuenong73-204' (low-tillering) x 'CP72-1210' (high-tillering). A genetic map spanning 1578.04 cM was constructed using 572 SNP identified via chip array genotyping. Through quantitative trait loci (QTL) mapping with LOD > 3, five QTLs linked to T-rate and four to NCPH were identified. Meanwhile, one QTL consistently present in at least two environments and one QTL present in both tillering traits. These QTLs accounted for the explanatory rate of phenotypic variation (PVE), ranging from 7.7 to 11.1% for T-rate and 5.6% to 10.7% for NCPH. Additionally, we screened one representative loci (AX-171307910) with one excellent genotype (GG), based on the QTL of qSE1/E2NCPH57 for NCPH, which excellent genotype will lead sugarcane toward better tillering traits. Finally, seven having this excellent genotype were screened. Further analysis revealed 3 relatively reliable candidate genes within the QTL of qSE1/E2NCPH57, including Soffic.01G0055400-1P, Soffic.01G0055410-1A, and Soffic.01G0055150-1A, respectively. However, RT-qPCR analysis discovered that the expression level of Soffic.01G0055400-1P and Soffic.01G0055150-A showed significant difference between Yuenong73-204 and CP72-1210, suggesting these two candidate genes were highly likely to be important genes affecting tillering. These insights offer valuable reference points for genetic enhancement strategies targeting tillers and for effective stalk development in sugarcane.
關鍵字 ：
Candidate genes Candidate genes Quantitative trait loci Quantitative trait loci Sugarcane Sugarcane Tillering Tillering
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| GB/T 7714 | Fang, Junteng , Lai, Ruiqiang , Chachar, Zaid et al. Identifying Novel QTLs and Candidate Genes for Tillering Ability in Sugarcane (Saccharum spp.) [J]. | SUGAR TECH , 2025 , 27 (3) : 798-810 . |
| MLA | Fang, Junteng et al. "Identifying Novel QTLs and Candidate Genes for Tillering Ability in Sugarcane (Saccharum spp.)" . | SUGAR TECH 27 . 3 (2025) : 798-810 . |
| APA | Fang, Junteng , Lai, Ruiqiang , Chachar, Zaid , Gui, Yiyun , Fan, Lina , Lin, Huanzhang et al. Identifying Novel QTLs and Candidate Genes for Tillering Ability in Sugarcane (Saccharum spp.) . | SUGAR TECH , 2025 , 27 (3) , 798-810 . |
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| GB/T 7714 | Wu, Qibin , Chen, Yanling , Zou, Wenhui et al. Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023) [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 295 . |
| MLA | Wu, Qibin et al. "Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023)" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 295 (2025) . |
| APA | Wu, Qibin , Chen, Yanling , Zou, Wenhui , Pan, Yong-Bao , Lin, Peixia , Xu, Liping et al. Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance (vol 232, 123398, 2023) . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 295 . |
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摘要 ：
Disease resistance is one of the most important target traits for sugarcane genetic improvement. Sugarcane brown stripe (SBS) caused by Helminthosporium stenospilum is one of the most destructive foliar diseases, which not only reduces harvest cane yield but also sugar content. This study aimed to identify quantitative trait loci (QTL) and candidate genes associated with SBS resistance. Here, the phenotypic investigation in six field habitats showed a continuous normal distribution, revealing that the SBS resistance trait is a quantitative trait. Two high-density linkage maps based on the single-dose markers calling from the Axiom Sugarcane100K SNP chip were constructed for the dominant sugarcane cultivars YT93-159 (SBS-resistant) and ROC22 (SBS-susceptible) with a density of 2.53 cM and 2.54 cM per SNP marker, and mapped on 87 linkage groups (LGs) and 80 LGs covering 3069.45 cM and 1490.34 cM of genetic distance, respectively. A total of 32 QTL associated with SBS resistance were detected by QTL mapping, which explained 3.73-11.64% of the phenotypic variation, and the total phenotypic variance explained (PVE) in YT93-159 and ROC22 was 107.44% and 79.09%, respectively. Among these QTL, four repeatedly detected QTL (qSBS-Y38-1, qSBS-Y38-2, qSBS-R8, and qSBS-R46) were considered stable QTL. Meanwhile, two major QTL, qSBS-Y38 and qSBS-R46, could account for 11.47% and 11.64% of the PVE, respectively. Twenty-five disease resistance candidate genes were screened by searching these four stable QTL regions in their corresponding intervals, of which Soffic.01G0010840-3C (PR3) and Soffic.09G0017520-1P (DND2) were significantly up-regulated in YT93-159 by qRT-PCR, while Soffic.01G0040620-1P (EDR2) was significantly up-regulated in ROC22. These results will provide valuable insights for future studies on sugarcane breeding in combating this disease.
關鍵字 ：
candidate gene candidate gene genetic map genetic map QTL mapping QTL mapping SBS resistance SBS resistance sugarcane brown stripe sugarcane brown stripe
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| GB/T 7714 | Cheng, Wei , Wang, Zhoutao , Xu, Fu et al. Mapping QTL and Identifying Candidate Genes for Resistance to Brown Stripe in Highly Allo-Autopolyploid Modern Sugarcane [J]. | HORTICULTURAE , 2025 , 11 (8) . |
| MLA | Cheng, Wei et al. "Mapping QTL and Identifying Candidate Genes for Resistance to Brown Stripe in Highly Allo-Autopolyploid Modern Sugarcane" . | HORTICULTURAE 11 . 8 (2025) . |
| APA | Cheng, Wei , Wang, Zhoutao , Xu, Fu , Yang, Yingying , Fang, Jie , Wu, Jianxiong et al. Mapping QTL and Identifying Candidate Genes for Resistance to Brown Stripe in Highly Allo-Autopolyploid Modern Sugarcane . | HORTICULTURAE , 2025 , 11 (8) . |
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摘要 ：
本发明涉及生物技术领域，特别是涉及一种检测甘蔗黄叶病毒的单克隆抗体及其应用和检测方法。所述单克隆抗体的轻链的可变区序的核苷酸序列如SEQ ID No.1所示，氨基酸序列如SEQ ID No.2所示；所述单克隆抗体的重链的可变区序列如SEQ ID No.3所示，氨基酸序列如SEQ ID No.4所示。本发明获得检测甘蔗黄叶病毒的单克隆抗体，并直接测出抗体的轻重链的可变区序列。与传统杂交瘤制备的单克隆相比，不用担心单克隆抗体的退化，突变或丢失。本发明根据甘蔗黄叶病毒的单克隆抗体作为一抗，建立检测方法来对甘蔗黄叶病毒进行检测。
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| GB/T 7714 | 许莉萍 , 王婷 . 一种检测甘蔗黄叶病毒的单克隆抗体及其应用和检测方法 : CN202510702207.1[P]. | 2025-05-28 . |
| MLA | 许莉萍 et al. "一种检测甘蔗黄叶病毒的单克隆抗体及其应用和检测方法" : CN202510702207.1. | 2025-05-28 . |
| APA | 许莉萍 , 王婷 . 一种检测甘蔗黄叶病毒的单克隆抗体及其应用和检测方法 : CN202510702207.1. | 2025-05-28 . |
| 導入鏈接 | NoteExpress RIS BibTex |
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摘要 ：
Sugarcane yellow leaf virus (SCYLV) can reduce sugarcane productivity. A novel detection system based on reverse transcription-multienzyme isothermal rapid amplification (RT-MIRA) combined with CRISPR-Cas12a, named RT-MIRA-CRISPR-Cas12a, was developed. This innovative approach employs crude leaf extract directly as the reaction template, streamlining the extraction process for simplicity and speed. Combining RT-MIRA and CRISPR-Cas12a in one reaction tube increases the ease of operation while reducing the risk of aerosol contamination. In addition, it exhibits sensitivity equivalent to qPCR, boasting a lower detection limit of 25 copies. Remarkably, the entire process, from sample extraction to reaction completion, requires only 52-57 minutes, just a thermostat water bath. The result can be observed and judged by the naked eye.
關鍵字 ：
CRISPR-Cas12a CRISPR-Cas12a MIRA MIRA sugarcane sugarcane Sugarcane yellow leaf virus Sugarcane yellow leaf virus
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| GB/T 7714 | Wang, Ting , Li, Anzhen , Zhao, Hong et al. A novel system with robust compatibility and stability for detecting Sugarcane yellow leaf virus based on CRISPR-Cas12a [J]. | MICROBIOLOGY SPECTRUM , 2024 , 12 (9) . |
| MLA | Wang, Ting et al. "A novel system with robust compatibility and stability for detecting Sugarcane yellow leaf virus based on CRISPR-Cas12a" . | MICROBIOLOGY SPECTRUM 12 . 9 (2024) . |
| APA | Wang, Ting , Li, Anzhen , Zhao, Hong , Wu, Qibin , Guo, Jinlong , Tian, Helei et al. A novel system with robust compatibility and stability for detecting Sugarcane yellow leaf virus based on CRISPR-Cas12a . | MICROBIOLOGY SPECTRUM , 2024 , 12 (9) . |
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摘要 ：
本发明属于生物技术领域，涉及一种快速培育转基因甘蔗的方法，尤其涉及一种利用甘蔗种茎快速获得转基因甘蔗的方法。针对现有甘蔗遗传转化周期时间久、组织培养工作量大的问题，直接利用甘蔗种茎，在超声波的作用下，通过农杆菌介导法进行甘蔗遗传转化，可快速高效地获得转基因甘蔗，比一般的农杆菌介导甘蔗遗传方法对比，转化时间可缩短50%以上，工作量减少80%以上。
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| GB/T 7714 | 许莉萍 , 杨颖颖 , 高世武 et al. 一种利用甘蔗种茎快速获得转基因甘蔗的方法 : CN202410054808.1[P]. | 2024-01-15 . |
| MLA | 许莉萍 et al. "一种利用甘蔗种茎快速获得转基因甘蔗的方法" : CN202410054808.1. | 2024-01-15 . |
| APA | 许莉萍 , 杨颖颖 , 高世武 , 郭晋隆 , 阙友雄 , 苏亚春 et al. 一种利用甘蔗种茎快速获得转基因甘蔗的方法 : CN202410054808.1. | 2024-01-15 . |
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摘要 ：
The processes of sugarcane tillering and ratooning, which directly affect the yield of plant cane and ratoon, are of vital importance to the population establishment and the effective stalk number per unit area. In the present study, the phenotypic data of 285 F-1 progenies from a cross of sugarcane varieties YT93-159 x ROC22 were collected in eight environments, which consisted of plant cane and ratoon cultivated in three different ecological sites. The broad sense heritability (H-2) of the tillering and the ratoon sprouting was 0.64 and 0.63, respectively, indicating that they were middle to middle-high heritable traits, and there is a significantly positive correlation between the two traits. Furthermore, a total of 26 quantitative trait loci (QTLs) related to the tillering ability and 11 QTLs associated with the ratooning ability were mapped on two high-quality genetic maps derived from a 100K SNP chip, and their phenotypic variance explained (PVE) ranged from 4.27-25.70% and 6.20-13.54%, respectively. Among them, four consistent QTLs of qPCTR-R9, qPCTR-Y28, qPCTR-Y60/qRSR-Y60 and PCTR-Y8-1/qRSR-Y8 were mapped in two environments, of which, qPCTR-Y8-1/qRSR-Y8 had the PVEs of 11.90% in the plant cane and 7.88% in the ratoon. Furthermore, a total of 25 candidate genes were identified in the interval of the above four consistent QTLs and four major QTLs of qPCTR-Y8-1, qPCTR-Y8-2, qRSR-R51 and qRSR-Y43-2, with the PVEs from 11.73-25.70%. All these genes were associated with tillering, including eight transcription factors (TFs), while 15 of them were associated with ratooning, of which there were five TFs. These QTLs and genes can provide a scientific reference for genetic improvement of tillering and ratooning traits in sugarcane.
關鍵字 ：
linkage marker linkage marker QTL mapping QTL mapping ratooning ratooning sugarcane sugarcane tillering tillering
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| GB/T 7714 | Wang, Ting , Xu, Fu , Wang, Zhoutao et al. Mapping of QTLs and Screening Candidate Genes Associated with the Ability of Sugarcane Tillering and Ratooning [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (3) . |
| MLA | Wang, Ting et al. "Mapping of QTLs and Screening Candidate Genes Associated with the Ability of Sugarcane Tillering and Ratooning" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 3 (2023) . |
| APA | Wang, Ting , Xu, Fu , Wang, Zhoutao , Wu, Qibin , Cheng, Wei , Que, Youxiong et al. Mapping of QTLs and Screening Candidate Genes Associated with the Ability of Sugarcane Tillering and Ratooning . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (3) . |
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摘要 ：
Inplants, lysine acetylation (Kac), 2-hydroxyisobutyrylation (Khib),and lysine lactylation (Kla), the three new types of post-translationalmodification (PTM), play very important roles in growth, development,and resistance to adverse environmental stresses. Herein, we reportthe first global acetylome, 2-hydroxyisobutyrylome, and lactylomein sugarcane. A total of 8573 Kac, 4637 Khib, and 215 Kla sites across3903, 1507, and 139 modified proteins were identified. Besides, homologyanalyses revealed the Kac, Khib, and Kla sites on histones were conservedbetween sugarcane and rice or poplar. Functional annotations demonstratedthat the Kac, Khib, and Kla proteins were mainly involved in energymetabolism. In addition, a number of modified transcription factorsand stress-related proteins, which were constitutively expressed indifferent tissues of sugarcane and induced by drought, cold or Sporisorium scitamineum stress, were identified.Finally, a proposed working mode on how PTM functions in sugarcanewas depicted. We thus concluded that PTM should play a role in sugarcanegrowth, development, and response to biotic and abiotic stresses,but the mechanisms require further investigation. The present studyprovided the all-new comprehensive profile of proteins Kac, Khib,and Kla and a new perspective to understand the molecular mechanismsof protein PTMs in sugarcane.
關鍵字 ：
lysine 2-hydroxyisobutyrylation lysine 2-hydroxyisobutyrylation lysine acetylation lysine acetylation lysine lactylation lysine lactylation modified proteins modified proteins post-translationalmodification post-translationalmodification sugarcane sugarcane
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| GB/T 7714 | Wu, Qibin , Li, Zhenxiang , Yang, Jingtao et al. Deciphering the Atlas of Post-Translational Modification in Sugarcane [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2023 , 71 (26) : 10004-10017 . |
| MLA | Wu, Qibin et al. "Deciphering the Atlas of Post-Translational Modification in Sugarcane" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 71 . 26 (2023) : 10004-10017 . |
| APA | Wu, Qibin , Li, Zhenxiang , Yang, Jingtao , Xu, Fu , Fu, Xueqin , Xu, Liping et al. Deciphering the Atlas of Post-Translational Modification in Sugarcane . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2023 , 71 (26) , 10004-10017 . |
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