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学者姓名：邓祖湖
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Abstract ：
Interspecific hybridization serves as a crucial strategy for innovating sugarcane germplasms. Currently, nearly all modern sugarcane varieties that incorporate genetic material are derived from Saccharum spontaneum. The number of chromosomes in S. spontaneum ranges from 40 to 128, contributing significantly to the diversity of its genetic resources. However, the genetic mechanisms driving chromosome number variation in S. spontaneum remain to be fully elucidated. Here, oligonucleotide fluorescence in situ hybridization (Oligo-FISH) was conducted to identify individual chromosomes and explore chromosome transmission during the intraspecific hybridization of S. spontaneum. The results indicate that from the progenies generated from S. spontaneum Yunnan2017-22 (2n = 8x = 64) and Yunnan82-1 (2n = 8x = 64) emerged two distinct karyotypes, 2n = 12x = 96 (A1) and 2n = 8x = 64 (A2, A33-1, A18). This implies that the chromosome inheritances were 2n + n and n + n in the progenies. However, self-pollinated samples of A1 (2n = 12x = 96) produced normal offspring C1 (2n = 94) and C2 (2n = 96). The 2n + n inheritance pattern did not continue. In another cross, the progenies derived from S. spontaneum Yunnan2017-41 (2n = 8x = 64) and Yunnan8 (2n = 10x = 80) carried a karyotype of 2n = 9x = 72, with n + n inheritance mode. These findings highlight the existence of two chromosome inheritance modes, 2n + n and n + n, in the context of the intraspecific hybridization of S. spontaneum. Additionally, hybridization between different ploidy S. spontaneum was also accompanied by chromosomal translocations (A1, A2, A18, A18) and loss (A2, A33-1, AA-4, and C2) that further resulted in the complexity of the S. spontaneum genome. Together, these findings highlight diverse chromosome inheritance in S. spontaneum hybridization, and provide a theoretical foundation for the further utilization of S. spontaneum germplasm in sugarcane breeding.
Keyword ：
chromosome inheritance chromosome inheritance chromosome karyotype chromosome karyotype Oligo-FISH Oligo-FISH S. spontaneum S. spontaneum
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| GB/T 7714 | Ran, Maoyong , Yu, Bo , Cheng, Chunxia et al. Oligo-FISH-Based Analysis of the Mechanisms Underlying Chromosome Number Variation in Saccharum spontaneum [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
| MLA | Ran, Maoyong et al. "Oligo-FISH-Based Analysis of the Mechanisms Underlying Chromosome Number Variation in Saccharum spontaneum" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 5 (2025) . |
| APA | Ran, Maoyong , Yu, Bo , Cheng, Chunxia , Li, Xueting , Guo, Yirong , Zhao, Liping et al. Oligo-FISH-Based Analysis of the Mechanisms Underlying Chromosome Number Variation in Saccharum spontaneum . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
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Chromosome fusion can cause a change in the basic chromosome number of a species, thus imposing a major impact on speciation. However, there are few studies on the structure and evolution of fusion chromosomes in plants. Erianthus rockii and Narenga porphyrocoma are allotetraploid species within the Saccharum complex. Both species possess five distinct fusion chromosomes, each resulting from the fusion of two ancestral chromosomes. We developed a high-resolution oligonucleotide (oligo)-based painting technique and constructed a detailed cytogenetic map for each of the 10 fusion chromosomes, enabling visualization of the breakpoints and fusion events of their ancestral chromosomes. We observed several common features associated with the 10 fusion chromosomes. The breakpoints of these fusion chromosomes were consistently located in the proximal regions near the centromeres of the ancestral chromosomes. Each fusion event was accompanied by the inactivation of one of the two ancestral centromeres, along with the loss of the associated centromeric repeats in the inactivated centromere. We conclude that the 10 fusion chromosomes likely originated through different mechanisms, including nested chromosome fusion, end-to-end fusion, and centromere misdivision followed by rejoining of the resulting fragments.
Keyword ：
centromere inactivation centromere inactivation chromosome fusion chromosome fusion chromosome painting chromosome painting oligo-FISH oligo-FISH sugarcane sugarcane
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| GB/T 7714 | Yu, Zehuai , Huang, Yongji , Yu, Xikai et al. Formation and centromere inactivation of fusion chromosomes in two allotetraploid species from the Saccharum complex [J]. | NEW PHYTOLOGIST , 2025 , 247 (6) : 2697-2708 . |
| MLA | Yu, Zehuai et al. "Formation and centromere inactivation of fusion chromosomes in two allotetraploid species from the Saccharum complex" . | NEW PHYTOLOGIST 247 . 6 (2025) : 2697-2708 . |
| APA | Yu, Zehuai , Huang, Yongji , Yu, Xikai , Deng, Zuhu , Chai, Jin , Liu, Jiarui et al. Formation and centromere inactivation of fusion chromosomes in two allotetraploid species from the Saccharum complex . | NEW PHYTOLOGIST , 2025 , 247 (6) , 2697-2708 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr03‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅3号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 邓祖湖 , 刘家睿 , 徐良年 et al. 一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法 : CN202510102827.1[P]. | 2025-01-22 . |
| MLA | 邓祖湖 et al. "一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法" : CN202510102827.1. | 2025-01-22 . |
| APA | 邓祖湖 , 刘家睿 , 徐良年 , 赵新旺 , 李雪停 , 黄国强 . 一种快速鉴定甘蔗是否含有斑茅3号染色体的PCR引物、试剂盒及检测方法 : CN202510102827.1. | 2025-01-22 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr02‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅2号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 邓祖湖 , 徐良年 , 赵新旺 et al. 一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法 : CN202510103245.5[P]. | 2025-01-22 . |
| MLA | 邓祖湖 et al. "一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法" : CN202510103245.5. | 2025-01-22 . |
| APA | 邓祖湖 , 徐良年 , 赵新旺 , 黄国强 , 李雪停 , 黄潮华 . 一种快速鉴定甘蔗是否含有斑茅2号染色体的PCR引物、试剂盒及检测方法 : CN202510103245.5. | 2025-01-22 . |
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Saccharum spontaneum serves as an essential genetic resource for sugarcane improvement. Traditional breeding methods, characterized by slow selection and limited germplasm exploitation, often lead to suboptimal progeny performance. In this study, we revised the utilization strategy by initially hybridizing several S. spontaneum clones, followed by intercrossing their F1 progeny to establish a heterogeneous 'polymeric' population, which was then subjected to 'nobilization'. A natural Saccharum spontaneum (S0) plant was used as the parent to create a hybrid (S1) containing two S. spontaneum bloodlines. The agronomic traits of S1 were compared, leading to the identification of three superior hybrids. These hybrids were then crossed in a complete diallel design, resulting in six crosses. Significant genetic variation was observed for the agronomic traits. Compared with S0, the plant height in S1 increased by 31.5%, and by 32.22% in S2. The stem diameter in S1 increased by 38.71%, and by 51.61% in S2. The single stem weight increased by 125% in S1 and 150% in S2. Other yield traits also showed varying degrees of improvement. A correlation analysis indicated that the plant height, stalk diameter, single stalk weight, and leaf width were significantly positively correlated with yield, and the leaf width with brix. There was no significant correlation between the millable stalks and yield. This study successfully developed a novel S. spontaneum hybrid with significantly improved agronomic traits, enhancing the genetic foundation of S. spontaneum germplasm for nobilization breeding programs. These findings provide a valuable germplasm base for developing high-performance sugarcane varieties, improving the utilization of S. spontaneum.
Keyword ：
genetic variation genetic variation hybrid hybrid Saccharum spontaneum Saccharum spontaneum sugarcane breeding sugarcane breeding sugarcane improvement sugarcane improvement
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| GB/T 7714 | Liu, Jiayong , Ran, Maoyong , Zhao, Liping et al. The Enhancement of a Saccharum spontaneum Population and a Genetic Impact Analysis of the Agronomic and Yield Traits of Its Progeny [J]. | PLANTS-BASEL , 2025 , 14 (12) . |
| MLA | Liu, Jiayong et al. "The Enhancement of a Saccharum spontaneum Population and a Genetic Impact Analysis of the Agronomic and Yield Traits of Its Progeny" . | PLANTS-BASEL 14 . 12 (2025) . |
| APA | Liu, Jiayong , Ran, Maoyong , Zhao, Liping , Tao, Lianan , Zan, Fenggang , Yao, Li et al. The Enhancement of a Saccharum spontaneum Population and a Genetic Impact Analysis of the Agronomic and Yield Traits of Its Progeny . | PLANTS-BASEL , 2025 , 14 (12) . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr05‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅5号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 刘家睿 , 邓祖湖 , 赵新旺 et al. 一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法 : CN202510102916.6[P]. | 2025-01-22 . |
| MLA | 刘家睿 et al. "一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法" : CN202510102916.6. | 2025-01-22 . |
| APA | 刘家睿 , 邓祖湖 , 赵新旺 , 徐良年 , 李雪停 , 黄潮华 . 一种快速鉴定甘蔗是否含有斑茅5号染色体的PCR引物、试剂盒及检测方法 : CN202510102916.6. | 2025-01-22 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr01‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅1号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 邓祖湖 , 赵新旺 , 郭哲君 et al. 一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法 : CN202510102438.9[P]. | 2025-01-22 . |
| MLA | 邓祖湖 et al. "一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法" : CN202510102438.9. | 2025-01-22 . |
| APA | 邓祖湖 , 赵新旺 , 郭哲君 , 徐良年 , 李雪停 , 刘家睿 . 一种快速鉴定甘蔗是否含有斑茅1号染色体的PCR引物、试剂盒及检测方法 : CN202510102438.9. | 2025-01-22 . |
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本发明公开了一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法。所述PCR引物为TaChr04‑F/R，其核苷酸序列如SEQ ID NO:1~2所示，通过该PCR引物，只需采用普通的PCR技术即可快速检测甘蔗材料是否含有斑茅4号染色体。本发明的检测方法效率高、稳定性好、操作简便，具有很强实用性强和推广性。
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| GB/T 7714 | 徐良年 , 邓祖湖 , 郭哲君 et al. 一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法 : CN202510102879.9[P]. | 2025-01-22 . |
| MLA | 徐良年 et al. "一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法" : CN202510102879.9. | 2025-01-22 . |
| APA | 徐良年 , 邓祖湖 , 郭哲君 , 黄潮华 , 李雪停 , 赵新旺 . 一种快速鉴定甘蔗是否含有斑茅4号染色体的PCR引物、试剂盒及检测方法 : CN202510102879.9. | 2025-01-22 . |
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斑茅是甘蔗重要的野生种质资源,渗入斑茅血缘来提高甘蔗抗性是目前甘蔗育种的重要途径之一。对甘蔗与斑茅杂交后代进行染色体分型有利于高效利用斑茅的各种优异性状。本研究利用斑茅特异引物鉴定斑茅与甘蔗杂交高世代材料的真实性,通过荧光原位杂交对甘蔗与斑茅染色体进行分型,以探究斑茅和甘蔗染色体在子代中的遗传与分离,并分析其染色体组结构特征。结果表明,杂交群体中有30份为斑茅的真实后代,包含斑茅染色体从1～10条不等,说明其后代群体基本服从n+n的遗传方式,占整个真实后代群体的60%。斑茅与甘蔗发生染色体水平的重组约16.67%,并且斑茅与不同血缘甘蔗重组的概率趋近一致。割手密种特异探针共定位结果表明,斑茅血缘的渗入降低了近现代栽培甘蔗种中热带种血缘与重组血缘的占比,并同时提高了割手密血缘的比例。本研究分析的甘蔗与斑茅杂交后代中不同血缘染色体组的遗传及结构特点,为提高斑茅种质资源在甘蔗育种中的开发利用提供了细胞遗传学基础。
Keyword ：
斑茅 斑茅 染色体遗传 染色体遗传 甘蔗 甘蔗 荧光原位杂交 荧光原位杂交
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| GB/T 7714 | 薛丽 , 李心怡 , 黄勇泰 et al. 甘蔗与斑茅杂交染色体组构成特征研究 [J]. | 作物学报 , 2024 , 50 (03) : 633-644 . |
| MLA | 薛丽 et al. "甘蔗与斑茅杂交染色体组构成特征研究" . | 作物学报 50 . 03 (2024) : 633-644 . |
| APA | 薛丽 , 李心怡 , 黄勇泰 , 欧财篮 , 吴小青 , 余泽怀 et al. 甘蔗与斑茅杂交染色体组构成特征研究 . | 作物学报 , 2024 , 50 (03) , 633-644 . |
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Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol, so increasing its yield is essential to ensure the sugar security and bioenergy production. Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants, particularly those species with high ploidy such as sugarcane (Saccharum spp.). Tripidium arundinaceum exhibits many desirable agronomic traits, and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding. However, the genetic relationship between T. arundinaceum and Saccharum species, and the individual T. arundinaceum chromosomal compositions in sugarcane hybrids are still elusive. Here we used whole-genome single-nucleotide polymorphisms (SNPs) to ascertain the phylogenetic relationships between these species and found that T. arundinaceum is more closely related to Saccharum than Sorghum, in contrast to the previous narrow derived from Saccharum officinarum was able to distinctly identify the chromosomes of T. arundinaceum. We developed the oligo-genomic in situ hybridization (GISH) system for the first time, to unveil the novel chromosome translocations and the transmission of individual T. arundinaceum chromosomes in sugarcane progeny. Notably, we discovered that the chromosomal transmission of T. arundinaceum exhibited several different inheritance modes, including n, 2n, and over 2n in the BC1 1 progenies. Such inheritance patterns may have resulted from first division restitution (FDR) or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/ second division restitution (FDR+NSC/SDR) model during meiosis. These results will be of substantial benefit for the further selection of T. arundinaceum chromosomes for sugarcane genetic improvement.
Keyword ：
chromosomal transmission chromosomal transmission chromosome painting chromosome painting Oligo-FISH Oligo-FISH sugarcane sugarcane Tripidium arundinaceum Tripidium arundinaceum
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| GB/T 7714 | Yu, Fan , Yu, Zehuai , Chai, Jin et al. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny [J]. | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (11) : 3751-3762 . |
| MLA | Yu, Fan et al. "Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny" . | JOURNAL OF INTEGRATIVE AGRICULTURE 23 . 11 (2024) : 3751-3762 . |
| APA | Yu, Fan , Yu, Zehuai , Chai, Jin , Yu, Xikai , Fu, Chen , Zhao, Xinwang et al. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny . | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (11) , 3751-3762 . |
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