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学者姓名：王锦达
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Abstract ：
Plants have developed various resistance mechanisms against herbivorous insects through prolonged coevolution. Plant defence responses can be triggered by specific compounds present in insect saliva. Apyrase, a known enzyme that catalyzes the hydrolysis of adenosine triphosphate (ATP) and adenosine diphosphate (ADP) into adenosine monophosphate (AMP) and inorganic phosphorus, has recently been identified in some herbivorous insects. However, whether insect salivary apyrase induces or inhibits plant responses remains poorly understood. In this study, we identified an apyrase-like protein in the salivary proteome of the fall armyworm, Spodoptera frugiperda, named Sfapyrase. Sfapyrase was primarily expressed in the salivary gland and secreted into plants during insect feeding. Transient expression of Sfapyrase in tobacco and maize enhanced plant resistance and resulted in decreased insect feeding. Knockdown of Sfapyrase through RNA interference led to increased growth and feeding of S. frugiperda. Furthermore, we showed that Sfapyrase activates the jasmonic acid signalling pathway and promotes the synthesis of secondary metabolites, especially benzoxazinoids, thereby enhancing resistance to S. frugiperda. In summary, our findings demonstrated that Sfapyrase acts as a salivary elicitor, inducing maize jasmonic acid defence responses and the production of insect-resistant benzoxazinoids. This study provides valuable insights into plant-insect interactions and offers potential targets for developing innovative insect pest management strategies.
Keyword ：
apyrase apyrase benzoxazinoids benzoxazinoids jasmonic acid jasmonic acid plant defence plant defence salivary elicitor salivary elicitor Spodoptera frugiperda Spodoptera frugiperda
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| GB/T 7714 | Yao, Yang , Lin, Huan-tai , Chen, Yao-hui et al. Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (1) : 406-420 . |
| MLA | Yao, Yang et al. "Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response" . | PLANT CELL AND ENVIRONMENT 48 . 1 (2025) : 406-420 . |
| APA | Yao, Yang , Lin, Huan-tai , Chen, Yao-hui , Chen, Li-lan , Zhang, Hui-li , Fu, Hua-ying et al. Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (1) , 406-420 . |
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Tuta absoluta is a significant agricultural pest primarily affecting Solanaceae plants, resulting in substantial economic losses in agriculture. Insect saliva is an intermediary between insects and plants, playing a crucial role in modulating host adaptability and plant defense. This study analyzed the adaptive differences of T. absoluta on four plants using the two-sex life table method. Results indicated that the host adaptability of T. absoluta to tobacco is worse than its adaptability to the other three varieties of tomatoes. The salivary gland transcriptome analysis and signal peptide prediction revealed that Trypsin, B5 V51-1498, and Ta74 were highly expressed in the salivary glands of T. absoluta subjected to tobacco treatment and exhibit the characteristics of secretory proteins, alongside significant feeding selection differences. Our findings elucidate the adaptive strategies of T. absoluta larvae on various Solanaceae plants and offer new insights into the salivary protein-mediated plant defense processes.
Keyword ：
host adaptation host adaptation interaction interaction salivary protein salivary protein transcriptome transcriptome Tuta absoluta Tuta absoluta
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| GB/T 7714 | Li, Xiujie , Cai, Xiangyun , Shang, Luohua et al. Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (5) : 2814-2829 . |
| MLA | Li, Xiujie et al. "Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 5 (2025) : 2814-2829 . |
| APA | Li, Xiujie , Cai, Xiangyun , Shang, Luohua , Wang, Yuzhou , Haq, Inzamam Ul , Wang, Jinda et al. Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (5) , 2814-2829 . |
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The invasion of the fall armyworm poses substantial threats to local agricultural safety, including the sugarcane industry. Exploring the insect-resistance mechanism is crucial for breeding resistant varieties. This study selected three representative materials from the Saccharum genus-Saccharum officinarum L. (Badila), S. spp. hybrid (GT58), and Saccharum spontaneum (SES208), to investigate feeding preferences and developmental fitness of Spodoptera frugiperda. Larvae exhibited a strong preference and highest fitness parameters in Badila leaves compared to SES208. Additionally, proteomic analyses of larval saliva from insects feeding on Badila and SES208 were carried out to test the types and abundance of proteins on different hosts. Furthermore, we identified fatty acid-binding protein 1-like activates plant defense responses, while aldehyde dehydrogenase negatively suppressed plant defense response. Our findings suggest that the plasticity of salivary proteins induced by different host plants, and also offer new insights into the molecular interactions between S. frugiperda and plants.
Keyword ：
life table life table salivary gland salivary gland Spodoptera frugiperda Spodoptera frugiperda sugarcane sugarcane
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| GB/T 7714 | Zhang, Ya-Xin , Lin, Huan-tai , Zou, Zhi-Yuan et al. Feeding Preferences and Salivary Protein Profiles of Spodoptera frugiperda on Saccharum Species [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (4) : 2352-2363 . |
| MLA | Zhang, Ya-Xin et al. "Feeding Preferences and Salivary Protein Profiles of Spodoptera frugiperda on Saccharum Species" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 4 (2025) : 2352-2363 . |
| APA | Zhang, Ya-Xin , Lin, Huan-tai , Zou, Zhi-Yuan , Li, Wu-yue , Tong, Yi-lu , Zhang, Hui-li et al. Feeding Preferences and Salivary Protein Profiles of Spodoptera frugiperda on Saccharum Species . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (4) , 2352-2363 . |
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Spodoptera frugiperda possesses a remarkably broad host range and causes substantial economic losses in global agricultural production. Insect saliva secreted onto plant surfaces plays a vital role in regulating both plant defense responses and insect adaptation. However, the mechanisms by which S. frugiperdasalivary proteins that respond to plant defense responses and regulate host adaptation remain elusive. The two-sex life table evaluation indicates that the host adaptability of S. frugiperda varies under different feeding schemes. Transcriptome analysis of differentially expressed genes in the salivary glands revealed that PTL, CSP2, and SPI are specifically responsive to host plants but not to an artificial diet. In S. frugiperda fed on adaptive host plants, the primary secretory proteins PDI and esterase FE4 are involved in enhancing host adaptation. When fed on nonadaptive host plants, S. frugiperda invests more energy in synthesizing detoxification metabolic enzymes such as trypsin, SPSL, and CLE2A. This study offers valuable insights into the adaptation mechanisms of phytophagous insects and identifies candidate genes involved in insect-plant interactions, offering targets for sustainable pest control strategies.
Keyword ：
adaptive host plants adaptive host plants interaction interaction Spodoptera frugiperda Spodoptera frugiperda transcriptome transcriptome two-sex lifetable two-sex lifetable
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| GB/T 7714 | Cai, Xiang-Yun , Wang, Ya-Ru , Li, Xiu-Jie et al. Detecting the Role of Salivary Protein Genes in Spodoptera frugiperda Adaptation to Different Host Plants [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (36) : 22295-22310 . |
| MLA | Cai, Xiang-Yun et al. "Detecting the Role of Salivary Protein Genes in Spodoptera frugiperda Adaptation to Different Host Plants" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 36 (2025) : 22295-22310 . |
| APA | Cai, Xiang-Yun , Wang, Ya-Ru , Li, Xiu-Jie , Zhang, Zheng-Hao , Ul Haq, Inzamam , Wang, Jin-Da et al. Detecting the Role of Salivary Protein Genes in Spodoptera frugiperda Adaptation to Different Host Plants . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (36) , 22295-22310 . |
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本发明公开了一株甘蔗内生粘质沙雷氏菌及其应用，属于农业微生物技术领域。所述甘蔗内生粘质沙雷氏菌分类命名为粘质沙雷氏菌（Serratia marcescens）SM，已于2023年10月18日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20231923，保藏地址为湖北省武汉市武昌区八一路299号。所述粘质沙雷氏菌从甘蔗茎中分离的，其活体可以作为以粘质沙雷氏菌活体或其代谢产物具有以下作用：（1）对甘蔗上的粘虫幼虫具有杀虫作用；（2）对于甘蔗白条病和甘蔗梢腐病病菌具有抗菌作用；（3）具有促进甘蔗发芽生长的作用。
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| GB/T 7714 | 王锦达 , 林东江 , 高三基 et al. 一株甘蔗内生粘质沙雷氏菌及其应用 : CN202410055646.3[P]. | 2024-01-15 . |
| MLA | 王锦达 et al. "一株甘蔗内生粘质沙雷氏菌及其应用" : CN202410055646.3. | 2024-01-15 . |
| APA | 王锦达 , 林东江 , 高三基 , 张慧丽 , 傅华英 , 黄美婷 . 一株甘蔗内生粘质沙雷氏菌及其应用 : CN202410055646.3. | 2024-01-15 . |
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The microRNAs (miRNAs) play an important role in the regulation of gene expression, influencing the outcome of many biological processes in insects. The introduction of miRNA into the insect body can lead to pest developmental abnormalities indicating that miRNAs are potential targets for pest control. In this study, we employed small RNA sequencing to characterise and analyse the expression of miRNAs in the sugarcane shoot borer Chilo infuscatellus (Snellen). By integrating previously published transcriptome sequencing data, we predicted and identified miRNAs and their target genes involved in the ecdysone metabolic pathway. We confirmed the target relationships between Ci-miR-4, Ci-miR-5 and CiCYP18A1 by RT-qPCR and dual-luciferase reporter assays in vivo and in vitro. Additionally, injection of miRNA mimics reduced the transcripts of CiCYP18A1 and led to abnormal moulting, which is similar to the defect phenotype of depletion of CiCYP18A1 by dsRNA. Furthermore, we also mixed the synthesised Ci-miR-5 mimic into artificial diets and found similar stunting results of the injection method. These results demonstrated that Ci-miR-5 could target CiCYP18A1 to regulate the moulting process and the successful use of an insect miRNA for gene silencing studies in this sugarcane pest. The method provided an effective novel approach for future RNAi-based pest control strategies.
Keyword ：
Chilo infuscatellus Chilo infuscatellus ecdysone metabolism ecdysone metabolism miRNA regulation miRNA regulation moult moult
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| GB/T 7714 | Wang, Jin-da , Lin, Huan-tai , Yang, Zhi-han et al. The ci-miR-5 regulates the CiCYP18A1 to control moulting process of Chilo infuscatellus: Prospective target for pest control [J]. | INSECT MOLECULAR BIOLOGY , 2025 , 34 (5) : 817-830 . |
| MLA | Wang, Jin-da et al. "The ci-miR-5 regulates the CiCYP18A1 to control moulting process of Chilo infuscatellus: Prospective target for pest control" . | INSECT MOLECULAR BIOLOGY 34 . 5 (2025) : 817-830 . |
| APA | Wang, Jin-da , Lin, Huan-tai , Yang, Zhi-han , Chen, Li-Fei , Tong, Yi-lu , Wei, Ji-li et al. The ci-miR-5 regulates the CiCYP18A1 to control moulting process of Chilo infuscatellus: Prospective target for pest control . | INSECT MOLECULAR BIOLOGY , 2025 , 34 (5) , 817-830 . |
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Sugarcane (Saccharum spp. hybrid) is the primary sugar crop in China, which accounts for over 86 % of the nation's total sugar output. However, sugarcane cultivation faces increasing threats from different insect species, among which the stem borers stand out as the most destructive pests for sugarcane yield. These pests undergo multiple generations per year, leading to escalating infestations. In the current review, we first introduced the occurrence patterns and economic losses caused by stem borers in China. Then, we compared current integrated pest management (IPM) strategies for stem borers including chemical communication, biological control, pesticides and resistant varieties domestic and international. In light of scientific advancements, emerging technologies such as transgenic insect-resistant sugarcane, RNA-based pesticides, the Sterile Insect Technique (SIT) and AI-driven intelligent control technologies show promise for future pest control efforts. This review specifically focuses on sugarcane stem borers management, providing insights into regional pest dynamics, climate-adaptive control strategies and emerging biotechnological solutions tailored to future sugarcane pest management practices.
Keyword ：
Bt Bt IPM IPM Resistant varieties Resistant varieties SIT SIT Sugarcane borers Sugarcane borers
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| GB/T 7714 | Wang, Jin-Da , Lin, Huan-Tai , Shang, Xian-Kun et al. Current status and research progress of sugarcane stem borers management [J]. | EUROPEAN JOURNAL OF AGRONOMY , 2025 , 168 . |
| MLA | Wang, Jin-Da et al. "Current status and research progress of sugarcane stem borers management" . | EUROPEAN JOURNAL OF AGRONOMY 168 (2025) . |
| APA | Wang, Jin-Da , Lin, Huan-Tai , Shang, Xian-Kun , Shan, Hong-Li , Li, Ji-Hu , Pan, Xue-Hong et al. Current status and research progress of sugarcane stem borers management . | EUROPEAN JOURNAL OF AGRONOMY , 2025 , 168 . |
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Research on the interactions between herbivorous insects and plants, facilitated by insect secretions, has increasingly emphasized species with chewing mandibles over time. However, the molecular mechanisms underlying the interaction between Spodoptera frugiperda and plants remain poorly understood. In this study, we identified a protein disulfide isomerase (SfPDI) from the salivary glands of S. frugiperda that regulates the interaction between S. frugiperda and plants. We found that SfPDI is highly expressed in the salivary glands of S. frugiperda and is secreted into plants as a secretory protein. The RNAi revealed that SfPDI contributes to the growth and development of S. frugiperda on host plants, while its overexpression in tobacco induces necrosis in tobacco leaves and triggers a burst of reactive oxygen species (ROS). Differentially expressed genes suggested that SfPDI may suppresses the expression of plant JA by positively regulating MYC2 and TIFYs and negatively regulating WRKYs. Notably, SfPDI may modulate these high expression of receptors (NB-LRR, GL-RLK, and RLK) lead to hypersensitive response (HR) cell death and the accumulation of lignification of plant. This study provides a foundation for further exploring insect-plant interaction mechanisms and a theoretical basis for developing insect-resistant germplasm and environmentally friendly pest control strategies.
Keyword ：
HR HR Insect-plant interaction Insect-plant interaction Plant defense Plant defense Salivary protein Salivary protein S. frugiperda S. frugiperda
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| GB/T 7714 | Cai, Xiang-Yun , Tang, Hua-Tao , Wang, Yu-Zhou et al. Salivary effector SfPDI modulates plant defense responses to enhance foraging efficiency of Spodoptera frugiperda [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 308 . |
| MLA | Cai, Xiang-Yun et al. "Salivary effector SfPDI modulates plant defense responses to enhance foraging efficiency of Spodoptera frugiperda" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 308 (2025) . |
| APA | Cai, Xiang-Yun , Tang, Hua-Tao , Wang, Yu-Zhou , Ul Haq, Inzamam , Wang, Jin-Da , Hou, You-Ming . Salivary effector SfPDI modulates plant defense responses to enhance foraging efficiency of Spodoptera frugiperda . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 308 . |
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RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi.
Keyword ：
bacterial-expressed bacterial-expressed nanocarrier nanocarrier off-target effect off-target effect red imported fire ant red imported fire ant RNAi RNAi
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| GB/T 7714 | Wang, Jin-da , Chen, Yao-hui , Zhang, Ya-xin et al. Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (19) : 10936-10943 . |
| MLA | Wang, Jin-da et al. "Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 19 (2024) : 10936-10943 . |
| APA | Wang, Jin-da , Chen, Yao-hui , Zhang, Ya-xin , Lin, Jin-wen , Gao, San-ji , Tang, Bao-zhen et al. Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (19) , 10936-10943 . |
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Simple Summary Insect gut commensal bacteria play an important role in insect adaptation to host plants. Feeding host plants is the main reason affecting the biomarker gut bacteria of insects, and it is of great significance to screen out a bacterial biomarker that can be cultured in vitro for insect host adaptability and pest control. There were few reports on the adaptability of host plants mediated by gut bacteria to Tuta absoluta. In this study, the bacterial biomarkers were screened and cultured from the growth and development analysis of tomato and potato. It was found that the biomarker bacteria of different strains of T. absoluta were helpful in improving the adaptability of T. absoluta to the original host plants and laying a foundation for the study of the interaction mechanism between insects and plants mediated by gut bacteria.Abstract The insect gut bacteria play important roles in insect development and growth, such as immune defense, nutrient metabolism, regulating insect adaptations for plants, etc. The Tuta absoluta (Meyrick) is a destructive invasive pest that mainly feeds on solanaceae plants. However, the relationship between gut microflora and host adaption of T. absoluta remains to be known. In this study, we first compared the survival adaptability of T. absoluta feeding with two host plants (tomatoes and potatoes). The T. absoluta completed the generation cycle by feeding on the leaves of both plants. However, the larvae feeding on tomato leaves have shorter larvae durations, longer adult durations, and a greater number of egg production per female. After Single Molecular Real-Time (SMRT) sequencing, according to the LDA Effect Size (LEfSe) analysis, the gut bacterial biomarker of T. absoluta fed on tomato was Enterobacter cloacae and the gut bacterial biomarker of T. absoluta fed on potatoes was Staphylococcus gallinarum and Enterococcus gallinarum. Furthermore, a total of 6 and 7 culturable bacteria were isolated from the guts of tomato- and potato-treated T. absoluta, respectively. However, the isolated strains included bacterial biomarkers E. cloacae and S. gallinarum but not E. gallinarum. In addition, different stains bacterial biomarkers on T. absoluta feeding selection were also studied. E. cloacae enhanced the host preference of the SLTA (T. absoluta of tomato strain) for tomato but had no impact on STTA (T. absoluta of potato strain). S. gallinarum improved the host preference of STTA to a potato but did not affect SLTA. The results showed that the gut bacteria of T. absoluta were affected by exposure to different host plants, and the bacterial biomarkers played an important role in host adaptability. This study not only deepens our understanding of gut bacteria-mediated insect-plant interactions but also provides theoretical support for the development of environmentally friendly and effective agricultural pest control methods.
Keyword ：
bacterial biomarker bacterial biomarker host adaptability host adaptability insect gut bacteria insect gut bacteria SMRT SMRT Tuta absoluta Tuta absoluta
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| GB/T 7714 | Shang, Luo-Hua , Cai, Xiang-Yun , Li, Xiu-Jie et al. Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants [J]. | INSECTS , 2024 , 15 (10) . |
| MLA | Shang, Luo-Hua et al. "Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants" . | INSECTS 15 . 10 (2024) . |
| APA | Shang, Luo-Hua , Cai, Xiang-Yun , Li, Xiu-Jie , Wang, Yu-Zhou , Wang, Jin-Da , Hou, You-Ming . Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants . | INSECTS , 2024 , 15 (10) . |
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