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学者姓名：王恒波
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Abstract ：
Cold stress is a critical environmental factor adversely affecting plant growth and development. As a tropical-origin crop constituting the primary global source of sucrose, sugarcane (Saccharum spp. hybrid) exhibits particular vulnerability to suboptimal temperature conditions, with chilling injury substantially compromising its yield potential. Despite its agricultural significance, the molecular mechanisms underlying cold acclimation in sugarcane remain poorly characterized. Here, we report a cold-repressed 1R-MYB gene, ScMYB7, from sugarcane, whose promoter (pro-ScMYB7) contains multiple cis-acting elements, including two cytosine-phosphate diester-guanine (CpG) islands. Bisulfite sequencing PCR (BSP) and qPCR results showed that low-temperature treatment increased the methylation level of the CpG islands in the promoter to reduce the transcription of the ScMYB7 gene. The outcomes of GUS enzyme activity measurement of the promoter also indicated that low-temperature treatment inhibits the promoter's transcriptional activity, and methylation inhibitors could alleviate this inhibition. By generating transgenic Arabidopsis lines overexpressing ScMYB7, ScMYB7's roles in regulating cold tolerance were investigated. We observed that the transgenic plants reduced cold tolerance, featured by a decreased survival rate after recovery, fluctuated physiological traits, and significantly lower expression levels of the C-repeat binding factor (CBF)-dependent pathway genes (AtCBFs, AtCOR15, and AtRD29A). Yeast one-hybrid assays demonstrated direct binding of ScMYB7 to the AtCBF1 promoter, while repression of sugarcane ScDREB1A occurred indirectly. Furthermore, the dual-luciferase reporter assay indicated that ScMYB7 was able to inhibit the expression of the AtCBF1 or ScDREB1A. Taken together, we propose a model in which ScMYB7 acts as a repressor of cold tolerance via the CBF-dependent pathway. Under low-temperature stress, increased methylation of the pro-ScMYB7 promoter reduces ScMYB7 expression, thereby alleviating its repression of sugarcane DREB/CBF-type transcription factors and enhancing cold adaptation.
Keyword ：
CBF-dependent pathway CBF-dependent pathway cold tolerance cold tolerance MYB transcription factor MYB transcription factor promoter methylation promoter methylation sugarcane sugarcane
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| GB/T 7714 | Liu, Weiwei , Ou, Qiuyue , Feng, Meichang et al. Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) : 8973-8984 . |
| MLA | Liu, Weiwei et al. "Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation" . | PLANT CELL AND ENVIRONMENT 48 . 12 (2025) : 8973-8984 . |
| APA | Liu, Weiwei , Ou, Qiuyue , Feng, Meichang , Li, Yue , Huang, Linghan , Peng, Xuan et al. Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) , 8973-8984 . |
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Sugarcane is recognized as the fifth largest crop globally, supplying 80% of sugar and 40% of bioenergy production. However, sugarcane genetic research has significantly lagged behind other crops due to its complex genetic background, high ploidy (8-13 x), aneuploidy, limited flowering, and a long growth cycle (more than one year). Cross breeding began in 1887 following the discovery that sugarcane seeds could germinate. Both self-and cross-pollination and selection were conducted by sugarcane breeders, but new cultivars were often eliminated due to disease susceptibility. Within the Saccharum genus, different species possess variable numbers of chromosomes. Wild sugarcane species intercrossed with each other, leading to development of the 'Nobilization' breeding strategy, which significantly improved yield, sucrose, fiber content, and disease resistance, and accelerated genetic improvement of cultivars. In recent years, scientific achievements have also been made in sugarcane genome sequencing, molecular marker development, genetic linkage map construction, localization of quantitative trait locus (QTL), and trait-associated gene identification. This review focuses on the progress in sugarcane genetic research, analyzes the technical difficulties faced, presents opportunities and challenges, and provides guidance and references for future sugarcane genetics research and cultivar breeding. Finally, it offers directions for future on sugarcane genetics. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keyword ：
Interspecific hybridization Interspecific hybridization Nobilization breeding Nobilization breeding Non-Mendelian inheritance Non-Mendelian inheritance Sugarcane breeding Sugarcane breeding
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| GB/T 7714 | Wang, Hengbo , Pan, Yong-Bao , Wu, Mingxing et al. Sugarcane genetics: Underlying theory and practical application [J]. | CROP JOURNAL , 2025 , 13 (2) : 328-338 . |
| MLA | Wang, Hengbo et al. "Sugarcane genetics: Underlying theory and practical application" . | CROP JOURNAL 13 . 2 (2025) : 328-338 . |
| APA | Wang, Hengbo , Pan, Yong-Bao , Wu, Mingxing , Liu, Junhong , Yang, Shiwei , Wu, Qibin et al. Sugarcane genetics: Underlying theory and practical application . | CROP JOURNAL , 2025 , 13 (2) , 328-338 . |
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Red stripe, caused by the bacterial pathogen Paracidovorax avenae, poses a significant threat to the sugarcane industry. The Sugar Will Eventually be Exported Transporter (SWEET) gene family participates in plant-pathogen interactions. However, the specific mechanism underlying the interaction between SWEETs and the red stripe pathogen remains unclear. In this study, 17, 21, and 25 members of the SWEET gene family were identified from Saccharum spontaneum, S. officinarum, and Saccharum spp. hybrid, respectively. They were phylogenetically divided into four clades. Four members in clade III, especially ScSWEET11, showed significantly different expression patterns between red stripe-resistant and susceptible sugarcane varieties. Subsequently, the ScSWEET11 gene was isolated and overexpressed in tobacco, resulting in significant lesions when infected with P. avenae (Pa), and there was no substantial difference in lesion area compared to wild-type tobacco. Heterologous expression of ScSWEET11 demonstrated sucrose transport activity in yeast sugar transport mutants. Besides, pScSWEET11_I and pScSWEET11_II, the two types of SWEET11 promoters in Saccharum, were mined and found to originate from S. spontaneum and S. officinarum, respectively. Interestingly, both types of promoters were observed in the susceptible cultivar, while there was only pScSWEET11_II in the resistant one. Notably, the activity of pScSWEET11_I was much higher than that of pScSWEET11_II, particularly under ABA and P. avenae stress conditions. Yeast one-hybrid, dual-luciferase reporter, and transient overexpression assays indicated that the interaction between PaXopQ, PaXopAU, PaXopF2, and pScSWEET11_I led to more susceptibility by promoting the ScSWEET11 expression, while that between PaAvrRxo1, PaXopAU, and pScSWEET11_II resulted in higher resistance through suppressing the ScSWEET11 expression. Collectively, this study provided a good understanding of the regulatory network for the red stripe pathogen invading the host, offering a valuable research basis for molecular breeding of disease-resistant sugarcane.
Keyword ：
effectors effectors Paracidovorax avenae Paracidovorax avenae plant-microbe interactions plant-microbe interactions sugarcane sugarcane SWEETs SWEETs
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| GB/T 7714 | Liu, Junhong , Du, Cuicui , Zhao, Ping et al. The interplay between ScSWEET11 promoters and Paracidovorax avenae effectors regulate resistance in sugarcane [J]. | PLANT JOURNAL , 2025 , 122 (6) . |
| MLA | Liu, Junhong et al. "The interplay between ScSWEET11 promoters and Paracidovorax avenae effectors regulate resistance in sugarcane" . | PLANT JOURNAL 122 . 6 (2025) . |
| APA | Liu, Junhong , Du, Cuicui , Zhao, Ping , Yang, Shiwei , Zhong, Hui , Zang, Shoujian et al. The interplay between ScSWEET11 promoters and Paracidovorax avenae effectors regulate resistance in sugarcane . | PLANT JOURNAL , 2025 , 122 (6) . |
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NAP (NAC-like, activated by apetala3/pistillata)是转录因子NAC基因家族中一类参与调控植物生长发育、叶片衰老及响应激素和非生物胁迫应答的亚家族。本研究利用甘蔗割手密种基因组数据和生物信息学方法,首先,基于比较基因组学对NAP亚家族成员进行鉴定、系统进化分析、保守结构域及顺式调控元件预测;其次,克隆获得割手密种SsNAP2的等位基因SsNAP2a,分析该基因在不同生长发育阶段的表达及其在激素和非生物胁迫下的表达特征;最后,利用瞬时表达和亚细胞定位分析SsNAP2a基因的功能。结果表明,在割手密种基因组中共鉴定5个NAP亚家族成员,亚细胞定位预测所有成员编码蛋白均定位于细胞核上,这些基因的Ka/Ks比值均小于1,表明纯化选择在演化中起到关键作用。系统聚类分析表明, 5个代表性的被子植物(拟南芥、菠萝、水稻、玉米和高粱)与已报道的12个物种及甘蔗的NAP亚家族成员,共计46个,分为4个Clade,其演化的顺序Clade I> Clade II> Clade III> Clade IV。此外,在SsNAP亚家族成员的启动子区域预测到较多响应脱落酸和茉莉酸、低温等逆境胁迫的顺式作用元件,推测其参与多种激素和非生物胁迫相关应答通路。进一步,从割手密种SES208中克隆到SsNAP2a基因,该cDNA全长序列1173 bp (GenBank登录号为OQ335094),编码390个氨基酸残基,其与等位基因SsNAP2编码蛋白的序列相似性为97.70%,有10个氨基酸残基的差异,表明同源8倍体的割手密种等位基因序列差异较大。qRT-PCR分析表明,SsNAP2a基因在割手密种不同生长发育阶段中组成型表达,尤其在衰老的蔗皮和根中的表达量最高;在乙烯利(ethylene, ET)、脱落酸(abscisic acid, ABA)、4℃低温和40℃高温处理下,其表达量呈现显著诱导表达;而在8%聚乙二醇(polyethyleneglycol,PEG)胁迫下显著下调表达。亚细胞定位表明,SsNAP2a融合蛋白定位在细胞核上。瞬时过表达SsNAP2a基因7 d后,本氏烟(Nicotianabenthamiana)叶片有明显的卷曲、皱缩等衰老现象,ET合成相关基因(NtEFE26,NtAccdeaminase)显著上调表达,而水杨酸、茉莉酸和ABA合成相关基因(NtPR-1a/c、NtPR3和NtA...
Keyword ：
NAP基因 NAP基因 割手密种 割手密种 叶片衰老 叶片衰老 基因功能 基因功能 激素胁迫 激素胁迫 甘蔗 甘蔗
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| GB/T 7714 | 王恒波 , 冯春燕 , 张以星 et al. 甘蔗割手密种转录因子NAP亚家族的鉴定及SsNAP2a参与叶片衰老的功能分析 [J]. | 作物学报 , 2024 , 50 (01) : 110-125 . |
| MLA | 王恒波 et al. "甘蔗割手密种转录因子NAP亚家族的鉴定及SsNAP2a参与叶片衰老的功能分析" . | 作物学报 50 . 01 (2024) : 110-125 . |
| APA | 王恒波 , 冯春燕 , 张以星 , 谢婉婕 , 杜翠翠 , 吴明星 et al. 甘蔗割手密种转录因子NAP亚家族的鉴定及SsNAP2a参与叶片衰老的功能分析 . | 作物学报 , 2024 , 50 (01) , 110-125 . |
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【目的】甘蔗褐锈病是由黑顶柄锈菌（Puccinia melanocephala H. Sydow&P. Sydow）引起的最具破坏性的真菌病害之一，能造成蔗糖分降低10%—36%，严重威胁甘蔗产业发展。已有研究揭示抗褐锈病主效基因定位于Bru1区域，克隆其关键候选基因，并进行功能研究，为选育抗褐锈病甘蔗新品种提供重要的候选基因资源。【方法】利用PacBio SequelⅡ测序平台获得甘蔗栽培品种ROC22的contig水平基因组信息，鉴定到抗褐锈病Bru1区域，对其进行基因注释和克隆，分析其组织特异性和抗、感褐锈病甘蔗品种中的表达模式及其在烟草叶片的亚细胞定位和瞬时表达。【结果】基于Bru1位点区域紧密连锁的标记R12H16和9O20-F4，从该区域中注释到33个基因，结合典型抗病基因结构域，共筛选获得5个候选抗病基因，分别为Brrg99、Brrg103、Brrg108、Brrg115和Brrg116。以甘蔗栽培品种ROC22的cDNA为模板，克隆获得Brrg116的全长序列729 bp，编码242个氨基酸残基。将该基因序列分别比对甘蔗热带种和割手密种及二倍体近缘种高粱的基因组数据库，发现其与热带种和割手密种的同源基因序列相似性很高，达到98%以上，而与高粱的相似性为93.77%。系统进化树揭示该基因起源甘蔗割手密种。实时荧光定量PCR检测表明，Brrg116在甘蔗栽培品种的多个组织中组成型表达，尤其是在+1叶中的表达量最高，是蔗芽的9.8倍；在褐锈病菌侵染抗、感甘蔗栽培品种的6和72 h时，呈现显著差异表达。亚细胞定位结果显示，该基因编码蛋白定位于细胞膜上。在本氏烟（Nicotiana benthamiana）叶片中瞬时过表达Brrg116，其后接种茄病镰刀菌蓝色变种，二氨基联苯胺法（3,3′-diaminobenzidine,DAB）染色逐渐加深，超敏反应相关基因、水杨酸信号和乙烯信号途径中的相关基因持续上调表达。【结论】Brrg116在甘蔗不同组织中组成型表达，且在受褐锈病菌侵染的抗病甘蔗栽培品种中呈现快速的诱导表达。此外，过表达Brrg116引发水杨酸和乙烯等激素信号通路产生防御响应，推测该基因可能在提高植物的抗病性方面发挥重要调控作用。
Keyword ：
Bru1区域 Bru1区域 候选抗病基因 候选抗病基因 甘蔗 甘蔗 褐锈病 褐锈病 连锁标记 连锁标记
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| GB/T 7714 | 吴期滨 , 谢婉婕 , 钟惠 et al. 甘蔗抗褐锈病Bru1区域的鉴定及候选抗病基因的功能分析 [J]. | 中国农业科学 , 2024 , 57 (05) : 855-867 . |
| MLA | 吴期滨 et al. "甘蔗抗褐锈病Bru1区域的鉴定及候选抗病基因的功能分析" . | 中国农业科学 57 . 05 (2024) : 855-867 . |
| APA | 吴期滨 , 谢婉婕 , 钟惠 , 冯春燕 , 潘浩然 , 齐浥颖 et al. 甘蔗抗褐锈病Bru1区域的鉴定及候选抗病基因的功能分析 . | 中国农业科学 , 2024 , 57 (05) , 855-867 . |
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The NAC (NAM, ATAF, and CUC) is one of the largest transcription factor gene families in plants. In this study, 180, 141, and 131 NAC family members were identified from Saccharum complex, including S. officinarum, S. spontaneum, and Erianthus rufipilus. The Ka/Ks ratio of ATAF subfamily was all less than 1. Besides, 52 ATAF members from 12 representative plants were divided into three clades and there was only a significant expansion in maize. Surprisingly, ABA and JA cis-elements were abundant in hormonal response factor, followed by transcriptional regulator and abiotic stressor. The ATAF subfamily was differentially expressed in various tissues, under low temperature and smut pathogen treatments. Further, the ScATAF1 gene, with high expression in leaves, stem epidermis, and buds, was isolated. The encoded protein, lack of self-activation activity, was situated in the cell nucleus. Moreover, SA and JA stresses down-regulated the expression of this gene, while ABA, NaCl, and 4 degrees C treatments led to its up-regulation. Interestingly, its expression in the smut susceptible sugarcane cultivars was much higher than the smut resistant ones. Notably, the colors presented slight brown in tobacco transiently overexpressing ScATAF1 at 1 d after DAB staining, while the symptoms were more obvious at 3 d after inoculation with Ralstonia solanacearum, with ROS, JA, and SA signaling pathway genes significantly upregulated. We thus speculated ScATAF1 gene could negatively mediate hypersensitive reactions and produce ROS by JA and SA signaling pathways. These findings lay the groundwork for in-depth investigation on the biological roles of ATAF subfamily in sugarcane.
Keyword ：
ATAF members ATAF members NAC transcription factor NAC transcription factor Pathogen defense Pathogen defense Smut disease Smut disease Sugarcane Sugarcane
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| GB/T 7714 | Wang, Hengbo , Qin, Liqian , Feng, Chunyan et al. Pathogen resistance was negatively regulated by the NAC transcription factor ScATAF1 in sugarcane [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2024 , 213 . |
| MLA | Wang, Hengbo et al. "Pathogen resistance was negatively regulated by the NAC transcription factor ScATAF1 in sugarcane" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 213 (2024) . |
| APA | Wang, Hengbo , Qin, Liqian , Feng, Chunyan , Wu, Mingxing , Zhong, Hui , Liu, Junhong et al. Pathogen resistance was negatively regulated by the NAC transcription factor ScATAF1 in sugarcane . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2024 , 213 . |
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本发明专利属于甘蔗基因工程领域，具体涉及甘蔗黄锈病抗性基因ScWAKL2及其抗病育种应用。本发明通过从抗、感黄锈病甘蔗品种中分别克隆ScWAKL2基因，找到功能差异的原因，通过异源过表达的方法验证该基因的抗病分子功能，针对ScWAKL2基因在抗、感病甘蔗等位基因型中的差异，开发分子标记，利用该标记对现有甘蔗品种及育种亲本材料进行抗性鉴定，为后续利用分子标记辅助育种选育抗黄锈病甘蔗新品种奠定重要的研究基础。
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| GB/T 7714 | 王恒波 , 覃丽谦 , 阙友雄 et al. 甘蔗黄锈病抗性基因ScWAKL2及其抗病育种应用 : CN202410659031.1[P]. | 2024-05-27 . |
| MLA | 王恒波 et al. "甘蔗黄锈病抗性基因ScWAKL2及其抗病育种应用" : CN202410659031.1. | 2024-05-27 . |
| APA | 王恒波 , 覃丽谦 , 阙友雄 , 刘俊鸿 , 杨世韦 , 赵平 . 甘蔗黄锈病抗性基因ScWAKL2及其抗病育种应用 : CN202410659031.1. | 2024-05-27 . |
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本发明属于植物分子生物学技术及分子检测技术领域，具体涉及一种甘蔗转录因子基因NAC1‑1在蔗茎发育中的应用。所述甘蔗转录因子基因NAC1‑1是从蔗茎绵心的甘蔗品种中克隆得到，其核苷酸序列如SEQ ID NO.1所示，其编码的蛋白质由289个氨基酸残基组成，其中50~150位氨基酸残基为NAC基因家族典型的NAM结构域。本发明揭示NAC1‑1基因的高效表达可诱导蔗茎产生绵心表型，低表达或者不表达没有绵心蔗茎的出现。结果证明，NAC1‑1基因的低表达对于甘蔗蔗茎正常发育具有重要调控作用，也为选育非绵心甘蔗新品种提供了重要理论依据和候选靶标基因资源。
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| GB/T 7714 | 王恒波 , 张积森 , 吴明星 et al. 一种甘蔗转录因子基因NAC1-1在蔗茎发育中的应用 : CN202310566156.5[P]. | 2023-05-19 . |
| MLA | 王恒波 et al. "一种甘蔗转录因子基因NAC1-1在蔗茎发育中的应用" : CN202310566156.5. | 2023-05-19 . |
| APA | 王恒波 , 张积森 , 吴明星 , 黄国强 , 郭晋隆 . 一种甘蔗转录因子基因NAC1-1在蔗茎发育中的应用 : CN202310566156.5. | 2023-05-19 . |
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本发明涉及一种诱导甘蔗茎绵心发育的启动子ProSsNAC1‑1及其应用。所述启动子ProSsNAC1‑1是从蔗茎绵心的甘蔗割手密种中克隆得到，核苷酸序列如SEQ ID NO.1所示。分别将ProSsNAC1‑1和蔗茎实心的甘蔗热带种来源的启动子ProSoNAC1‑1与GUS基因构建融合表达载体，转化拟南芥，将T3代纯合转基因拟南芥植株分别在不同组织及低温和干旱胁迫下进行表达模式分析和组织化学染色。结果证实，启动子ProSsNAC1‑1比ProSoNAC1‑1具有较强的表达量；启动子ProSsNAC1‑1能驱动NAC1‑1基因在甘蔗叶脉和茎秆的维管束组织高效表达，且其受低温和干旱诱导。本发明的启动子ProSsNAC1‑1对甘蔗茎绵心发育调控、甘蔗品质改良具重要价值。
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| GB/T 7714 | 王恒波 , 吴明星 , 张积森 et al. 一种诱导甘蔗茎绵心发育的启动子及其应用 : CN202310567324.2[P]. | 2023-05-19 . |
| MLA | 王恒波 et al. "一种诱导甘蔗茎绵心发育的启动子及其应用" : CN202310567324.2. | 2023-05-19 . |
| APA | 王恒波 , 吴明星 , 张积森 , 黄国强 , 郭晋隆 . 一种诱导甘蔗茎绵心发育的启动子及其应用 : CN202310567324.2. | 2023-05-19 . |
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SWEET蛋白通过调控植物体内糖分的运输、分配、转化和贮藏,广泛参与植物生长发育及响应病原菌胁迫的生理生化过程。为揭示SWEET基因在甘蔗生长发育及其与赤条病菌互作中的生物学功能,本研究基于甘蔗割手密种全长转录组文库和比较基因组学,根据注释SsSWEET11基因序列设计特异引物,利用RT-PCR技术从甘蔗割手密种cDNA文库中扩增该基因的全长序列,运用多种生物信息工具分析其特征,构建系统进化树;采用不同组织和抗、感赤条病的甘蔗品种分析SsSWEET11的表达模式;利用瞬时表达和亚细胞定位分析SsSWEET11的功能。结果表明,从甘蔗割手密种克隆获得SsSWEET11基因(登录号为OP554214),该基因全长927bp,编码308个氨基酸残基,具有2个MtN3＿saliva结构域和7次跨膜结构域。系统进化树分析显示,SsSWEET11属于SWEET蛋白家族第Ⅲ亚家族成员,与高粱SbSWEET11相似性高达97.41%。qRT-PCR分析表明, ShSWEET11基因在不同组织中组成型表达,在蔗叶和根中表达量显著高于其他组织;赤条病菌胁迫下,ScSWEET11基因在抗病品种ROC22和感病品种MT11-610中呈现完全不同的表达趋势,与对照相比,抗病品种中该基因的表达量显著下调,而感病品种中,在胁迫48h和72h后该基因的表达量显著上调,分别为对照的5.90倍和5.43倍。亚细胞定位表明, SsSWEET11-GFP融合蛋白定位在质膜上。瞬时过表达SsSWEET11基因1 d后,二氨基联苯胺(Diaminobenzidine, DAB)对本氏烟叶片进行染色,叶片颜色没有变化,再接种烟草青枯菌、茄病镰刀菌蓝色变种7 d后,过表达植株叶片发病比对照组严重,且过敏反应相关基因、茉莉酸和水杨酸代谢途径相关基因呈现上调表达,而乙烯通路相关基因则没有响应,表明SsSWEET11基因参与茉莉酸和水杨酸信号传导通路,且病原菌侵染本氏烟草叶片能够诱发过敏反应。研究结果不仅为开发与甘蔗抗赤条病菌性状关联的分子标记提供积累,也为深入解析赤条病侵染甘蔗的分子机制奠定一定的基础。
Keyword ：
SWEET基因 SWEET基因 割手密种 割手密种 基因功能 基因功能 甘蔗 甘蔗 糖转运蛋白 糖转运蛋白 赤条病菌 赤条病菌
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| GB/T 7714 | 杜翠翠 , 吴明星 , 张雅婷 et al. 甘蔗割手密种糖转运蛋白基因SsSWEET11的克隆与功能分析 [J]. | 作物学报 , 2023 , 49 (09) : 2385-2397 . |
| MLA | 杜翠翠 et al. "甘蔗割手密种糖转运蛋白基因SsSWEET11的克隆与功能分析" . | 作物学报 49 . 09 (2023) : 2385-2397 . |
| APA | 杜翠翠 , 吴明星 , 张雅婷 , 谢婉婕 , 张积森 , 王恒波 . 甘蔗割手密种糖转运蛋白基因SsSWEET11的克隆与功能分析 . | 作物学报 , 2023 , 49 (09) , 2385-2397 . |
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