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学者姓名：胡朝华
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Abstract ：
Conservative anti-cancer treatment represented by chemotherapy and surgery lacks tumor-specificity and could hardly resolve the problems associated with multidrug resistance (MDR) in cancers. Novel therapeutic materials in cancer treatment, such as those with anti-MDR or controllable treatment features, represent a significant trend due to their advantages of high and specific efficacy and timely intervention of cancer progress. In addition to their excellent biocompatibility and specificity, they can be utilized in therapies that require ease of operation, provided they are designed with high detection sensitivity. In this review, we summarize a series of recently developed materials that exhibit these advantages, including immune-enhancing and tumor microenvironment (TME)- responsive materials, and those with integrated therapeutic and imaging capabilities. We also introduce advanced modification approaches that can impart essential targeting functionalities to these materials.
Keyword ：
controllable treatment of cancer controllable treatment of cancer integrated cancer diagnosis integrated cancer diagnosis materials for enhancing immunity materials for enhancing immunity reversal of multidrug resistance (MDR) reversal of multidrug resistance (MDR) tumor microenvironment (TME)-responsive material tumor microenvironment (TME)-responsive material
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| GB/T 7714 | Zhang, Lei , Wang, Yanan , Li, Yangjia et al. Advanced materials for cancer treatment and beyond [J]. | FRONTIERS IN PHARMACOLOGY , 2025 , 16 . |
| MLA | Zhang, Lei et al. "Advanced materials for cancer treatment and beyond" . | FRONTIERS IN PHARMACOLOGY 16 (2025) . |
| APA | Zhang, Lei , Wang, Yanan , Li, Yangjia , Chen, Zhe-Sheng , Hu, Chaohua . Advanced materials for cancer treatment and beyond . | FRONTIERS IN PHARMACOLOGY , 2025 , 16 . |
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Background: PD-L1, an immune checkpoint inhibitor, and VEGFR2, essential for cancer metastasis, play pivotal roles in tumorigenesis. However, their miniature bispecific intracellular nanobodies for combining check-point blockade and anti-metastasis anticancer therapy remain underexplored. Methods: The intrabodies were developed using gene cloning technology. Specificity of the intrabodies was testified using Western blot, co-immunoprecipitation (co-IP) analysis, antibody competitive binding assay, flow cytometry analysis, etc. Checkpoint blockade was demonstrated using antibody-antigen competitive binding assay. Cancer cell migration was determined using scratch assay. Combined anti-cancer therapeutic efficacy of FAP1V2 was determined in vivo of mice models. The PD-1(hi) immune cells, TCR beta(hi) and CD25(hi) T-cells were analyzed by flow cytometry, and cancer cell metastasis was performed using immune-fluorescence analysis on lung and liver tissues. Transcriptome analysis was performed to explore signaling pathways associated with the enhanced anticancer efficiency. Results: Bispecific intrabody FAP1V2 fused with antibody V-H regions, was successfully developed and verified with its ability to target and block human and mouse PD-L1 and VEGFR2, inhibiting cancer cell binding to PD-1 and reducing their migratory capacity. Compared to the other treatment, two-rounds of transient FAP1V2 expression in LLC cells in experimental mice models achieved remarkable tumor inhibition, which brought about complete immune inhibition on growth of secondary-round of LLC tumor in 1/6 of the tested mice, inspired long-term activation of TCR beta(hi) T cells and increased their infiltration to tumors, inhibited the emergence of PD-1(hi) immune cells, indicating prevented T cell depletion. The elevated CD25 expression also supported the success in enhancing immune response reported by elevated T cell activity in spleen. Transcriptome analysis identified critical intracellular pathways regulated by the concurrent blockade of PD-L1 and VEGFR2. Conclusion: PD-L1 and VEGFR2- bispecific V-H intracellular nanobody was highly biocompatible and showed the potential for combined anti-cancer therapy through long-term immune activation mediated by PD-L1/PD-1 checkpoint blockade and anti-metastasis mediated by VEGFR2 blockade.
Keyword ：
Anti-metastasis Anti-metastasis Bispecific V-H intrabody Bispecific V-H intrabody Combination cancer therapy Combination cancer therapy Immune activation Immune activation PD-L1 PD-L1 T cell activation and infiltration T cell activation and infiltration VEGFR2 VEGFR2
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| GB/T 7714 | Zhang, Lei , Lin, Yunfeng , Hu, Li et al. Transient intracellular expression of PD-L1 and VEGFR2 bispecific nanobody in cancer cells inspires long-term T cell activation and infiltration to combat tumor and inhibit cancer metastasis [J]. | MOLECULAR CANCER , 2025 , 24 (1) . |
| MLA | Zhang, Lei et al. "Transient intracellular expression of PD-L1 and VEGFR2 bispecific nanobody in cancer cells inspires long-term T cell activation and infiltration to combat tumor and inhibit cancer metastasis" . | MOLECULAR CANCER 24 . 1 (2025) . |
| APA | Zhang, Lei , Lin, Yunfeng , Hu, Li , Wang, Yanan , Hu, Chaohua , Shangguan, Xinyi et al. Transient intracellular expression of PD-L1 and VEGFR2 bispecific nanobody in cancer cells inspires long-term T cell activation and infiltration to combat tumor and inhibit cancer metastasis . | MOLECULAR CANCER , 2025 , 24 (1) . |
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The diagnosis and treatment of cancers have become a significant challenge in overcoming malignant diseases. Early detection of tumors and timely targeted therapy can greatly impede the rapid deterioration of cancers. In recent years, nano-systems based on photodynamic materials have shown great progress in tumor diagnosis and treatment applications. With the continuous exploration of tumor-specific targets and the development of photodynamic nanoparticles, the generation of new nanoparticles that are target-specific, highly sensitive, and biosafe for integrated diagnosis and therapy is realistic. This review introduces the rational basis for photosensitizer-based materials for integrating cancer diagnosis and anti-cancer therapy, types and characteristics of organic and inorganic photosensitizers currently used for PDT treatment, photosensitive nano-materials with dual detection and therapeutic properties the advancement in developing photo-dynamic nano-systems showing potential in integrated diagnosis and therapeutic applications. We also introduce current strategies for optimizing nano-systems with the properties for enhancing targeting ROS release and accurate imaging, combining therapeutic efficacy, as well as biosafety of the integrative materials for PDT application, providing references for the coordinated optimization of photosensitizer design and clinical translation. Plain Language Summary: The integration of PDT with advanced diagnostic modalities represents a transformative approach in cancer theranostics, as evidenced by the comprehensive exploration of photosensitizer-based nanomaterials in this review. By leveraging the unique photophysical properties of organic and inorganic photosensitizers, researchers have developed innovative nano-systems capable of simultaneous tumor detection and targeted therapy. Key advancements include the rational design of aggregation-induced emission photosensitizers (AIE-PS) with redshifted absorption spectra, hypoxia-responsive nanomaterials for enhanced ROS generation, and multifunctional composites such as UCNP@MOF hybrids that address tumor microenvironment limitations. These systems demonstrate improved targeting precision, reduced off-target effects, and synergistic therapeutic outcomes when combined with chemotherapy or immunotherapy. Despite these strides, challenges persist in optimizing light penetration depth, mitigating photobleaching, and ensuring biosafety during clinical translation. The development of oxygen-independent Type III photosensitizers and stimuli-responsive delivery systems offers promising avenues to overcome hypoxia-related barriers. Furthermore, the integration of MOFs and UCNPs highlights the potential for real-time imaging-guided therapy. Future efforts should prioritize scalable synthesis, rigorous toxicological profiling, and combinatorial strategies to enhance therapeutic efficacy while minimizing systemic toxicity. By bridging nanotechnology, materials science, and clinical oncology, next-generation photodynamic platforms hold immense potential to redefine precision medicine in oncology and beyond. While PDT nanomaterials offer revolutionary potential in cancer theranostics, addressing toxicity, stability, and clinical scalability is critical. Integrating PDT with complementary modalities (chemotherapy, immunotherapy) and advancing TME-responsive designs will bridge the gap between preclinical innovation and clinical application.
Keyword ：
advanced imaging technology advanced imaging technology integrated diagnosis and treatment integrated diagnosis and treatment novel nano-systems novel nano-systems photodynamic therapy photodynamic therapy precision anti-cancer therapy precision anti-cancer therapy tumor targeting tumor targeting
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| GB/T 7714 | Wang, Yanan , Hu, Chaohua , Li, Yangjia et al. Advancing Cancer Treatment: Innovative Materials in PDT and Diagnostic Integration [J]. | INTERNATIONAL JOURNAL OF NANOMEDICINE , 2025 , 20 : 7037-7060 . |
| MLA | Wang, Yanan et al. "Advancing Cancer Treatment: Innovative Materials in PDT and Diagnostic Integration" . | INTERNATIONAL JOURNAL OF NANOMEDICINE 20 (2025) : 7037-7060 . |
| APA | Wang, Yanan , Hu, Chaohua , Li, Yangjia , Chen, Zhe-Sheng , Zhang, Lei . Advancing Cancer Treatment: Innovative Materials in PDT and Diagnostic Integration . | INTERNATIONAL JOURNAL OF NANOMEDICINE , 2025 , 20 , 7037-7060 . |
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Root-soil underground interactions mediated by soil microorganisms and metabolites are crucial for fertilizer utilization efficiency and crop growth regulation. This study employed a combined approach of soil microbial community profiling and non-targeted metabolomics to investigate the patterns of root-associated microbial aggregation and the mechanisms associated with metabolites under varying controlled-release fertilizer (CRF) application rates. The experimental treatments included five field application rates of CRF (D1: 675 kg/ha; D15: 1012.5 kg/ha; D2: 1350 kg/ha; D25: 1687.5 kg/ha; and D3: 2025 kg/ha) along with traditional fertilizer as a control (CK: 1687.5 kg/ha). The results indicated that the growth of sugarcane in the field was significantly influenced by the CRF application rate (p < 0.05). Compared with CK, the optimal field application of CRF was observed at D25, resulting in a 16.3% to 53.6% increase in sugarcane yield. Under the condition of reducing fertilizer application by 20%, D2 showed a 13.3% increase in stem yield and a 6.7% increase in sugar production. The bacterial ACE index exhibited significant differences between D25 and D1, while the Chao1 index showed significance among the D25, D1, and CK treatments. The dominant bacterial phyla in sugarcane rhizosphere aggregation included Proteobacteria, Actinobacteriota, and Acidobacteriota. Fungal phyla comprised Rozellomycota, Basidiomycota, and Ascomycota. The annotated metabolic pathways encompassed biosynthesis of secondary metabolites, carbohydrate metabolism, and lipid metabolism. Differential analysis and random forest selection identified distinctive biomarkers including Leotiomycetes, Cercospora, Anaeromyxobacter, isoleucyl-proline, and methylmalonic acid. Redundancy analysis unveiled soil pH, soil organic carbon, and available nitrogen as the primary drivers of microbial communities, while the metabolic profiles were notably influenced by the available potassium and phosphorus. The correlation heatmaps illustrated potential microbial-metabolite regulatory mechanisms under CRF application conditions. These findings underscore the significant potential of CRF in sugarcane field production, laying a theoretical foundation for sustainable development in the sugarcane industry.
Keyword ：
application rate application rate controlled release fertilizer controlled release fertilizer metabolites metabolites rhizosphere microorganisms rhizosphere microorganisms sugarcane sugarcane sugar industry sugar industry
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| GB/T 7714 | Yuan, Zhaonian , Liu, Qiang , Mo, Lifang et al. Integrating the Soil Microbiota and Metabolome Reveals the Mechanism through Which Controlled Release Fertilizer Affects Sugarcane Growth [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (18) . |
| MLA | Yuan, Zhaonian et al. "Integrating the Soil Microbiota and Metabolome Reveals the Mechanism through Which Controlled Release Fertilizer Affects Sugarcane Growth" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 18 (2023) . |
| APA | Yuan, Zhaonian , Liu, Qiang , Mo, Lifang , Pang, Ziqin , Hu, Chaohua . Integrating the Soil Microbiota and Metabolome Reveals the Mechanism through Which Controlled Release Fertilizer Affects Sugarcane Growth . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (18) . |
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Understanding the normal variation of the sugarcane rhizosphere fungal community throughout its life cycle is essential for the development of agricultural practices for fungal and ecological health associated with the microbiota. Therefore, we performed high-throughput sequencing of 18S rDNA of soil samples using the Illumina sequencing platform for correlation analysis of rhizosphere fungal community time series, covering information from 84 samples in four growth periods. The results revealed that the sugarcane rhizosphere fungi possessed the maximum fungal richness in Tillering. Rhizosphere fungi were closely associated with sugarcane growth, including Ascomycota, Basidiomycota, and Chytridiomycota, which showed high abundance in a stage-specific manner. Through the Manhattan plots, 10 fungal genera showed a decreasing trend throughout the sugarcane growth, and two fungal genera were significantly enriched at three stages of sugarcane growth (p < 0.05) including Pseudallescheria (Microascales, Microascaceae) and Nectriaceae (Hypocreales, Nectriaceae). In addition, soil pH, soil temperature, total nitrogen, and total potassium were critical drivers of fungal community structure at different stages of sugarcane growth. We also found that sugarcane disease status showed a significant and strong negative effect on selected soil properties by using structural equation modeling (SEM), suggesting that poor soil increases the likelihood of sugarcane disease. In addition, the assembly of sugarcane rhizosphere fungal community structure was mainly influenced by stochastic factors, but after the sugarcane root system became stable (Maturity), the stochastic contribution rate decreased to the lowest value. Our work provides a more extensive and solid basis for the biological control of sugarcane potential fungal diseases.
Keyword ：
fungi community assembly fungi community assembly rhizosphere fungi rhizosphere fungi soil properties soil properties structural equation modeling structural equation modeling temporal variation temporal variation
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| GB/T 7714 | Liu, Qiang , Pang, Ziqin , Liu, Yueming et al. Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (6) . |
| MLA | Liu, Qiang et al. "Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 6 (2023) . |
| APA | Liu, Qiang , Pang, Ziqin , Liu, Yueming , Fallah, Nyumah , Hu, Chaohua , Lin, Wenxiong et al. Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (6) . |
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RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency. In the current study, we identified four genes coding for dsRNases from the Spodoptera frugiperda genome. Spatial and temporal expression analysis showed that those dsRNases were highly expressed in the midgut and old larvae. Then a delivery method was applied for inducing efficient RNAi based on dsRNA encapsulated by liposome. Furthermore, we assessed degradation efficiency by incubation with dsRNA with gut juice or hemocoel to characterize potential roles of different SfdsRNases after suppression of SfdsRNase. The result showed that interferenced with any sfdsRNase reduced the degradation of exogenous dsRNA in midgut, interfered with sfdsRNase1 and sfdsRNase3 slowed down the degradation of exogenous dsRNA in hemolymph. Our data suggest the evolutionary expansion and multiple high activity dsRNase genes would take part in the RNAi obstinate in S. frugiperda, besides we also provide an efficient RNAi method for better use of RNAi in S. frugiperda.
Keyword ：
dsRNase dsRNase efficiency efficiency liposome liposome RNA inference RNA inference Spodoptera frugiperda Spodoptera frugiperda
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| GB/T 7714 | Yao, Yang , Lin, Dong-Jiang , Cai, Xiang-Yun et al. Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda [J]. | FRONTIERS IN PHYSIOLOGY , 2022 , 13 . |
| MLA | Yao, Yang et al. "Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda" . | FRONTIERS IN PHYSIOLOGY 13 (2022) . |
| APA | Yao, Yang , Lin, Dong-Jiang , Cai, Xiang-Yun , Wang, Ran , Hou, You-Ming , Hu, Chao-Hua et al. Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda . | FRONTIERS IN PHYSIOLOGY , 2022 , 13 . |
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Sugarcane-legume intercropping systems can effectively control pests and diseases as well as improve the fertility and health of farmland soil. However, little is known about the response of bacterial abundance, diversity, and community composition in the rhizosphere and non-rhizosphere soils under the sugarcane-peanut farming system. A field experiment was conducted with two treatments: sugarcane monoculture and sugarcane-peanut intercropping to examine the response of sugarcane parameters and edaphic factors. We also deciphered bacterial abundance, diversity, and community composition in the root endosphere, rhizosphere, and bulk soil by leveraging Illumina sequencing to conduct the molecular characterization of the 16S rRNA gene and nitrogenase (nifH) gene. We observed that sugarcane-peanut intercropping exhibited the advantages of tremendously increasing cane stalk height, stalk weight, and millable stalk number/20 m, and edaphic factors, namely, pH (1.13 and 1.93), and available phosphorus exhibited a fourfold and sixfold increase (4.66 and 6.56), particularly in the rhizosphere and bulk soils, respectively. Our result also showed that the sugarcane-peanut intercropping system significantly increased the bacterial richness of the 16S rRNA gene sequencing data by 13.80 and 9.28% in the bulk soil and rhizosphere soil relative to those in the monocropping sugarcane system, respectively. At the same time, sugarcane intercropping with peanuts significantly increased the Shannon diversity of nitrogen-fixing bacteria in the sugarcane rhizosphere soil. Moreover, most edaphic factors exhibited a positive regularity effect on bacterial community composition under the intercropping system. A linear discriminant analysis with effect size analysis of the 16S rRNA sequencing data revealed that bacteria in the root endosphere of the intercropped cane proliferated profoundly, primarily occupied by Devosia, Rhizobiales, Myxococcales, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Bradyrhizobium, and Sphingomonas. In conclusion, our findings demonstrated that sugarcane-peanut intercropping can enhance edaphic factors, sugarcane parameters, and bacterial abundance and diversity without causing adverse impacts on crop production and soil.
Keyword ：
bacterial diversity and abundance bacterial diversity and abundance endosphere endosphere intercropping intercropping rhizosphere and non-rhizosphere soil rhizosphere and non-rhizosphere soil sugarcane sugarcane
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| GB/T 7714 | Pang, Ziqin , Fallah, Nyumah , Weng, Peiying et al. Sugarcane-Peanut Intercropping System Enhances Bacteria Abundance, Diversity, and Sugarcane Parameters in Rhizospheric and Bulk Soils [J]. | FRONTIERS IN MICROBIOLOGY , 2022 , 12 . |
| MLA | Pang, Ziqin et al. "Sugarcane-Peanut Intercropping System Enhances Bacteria Abundance, Diversity, and Sugarcane Parameters in Rhizospheric and Bulk Soils" . | FRONTIERS IN MICROBIOLOGY 12 (2022) . |
| APA | Pang, Ziqin , Fallah, Nyumah , Weng, Peiying , Zhou, Yongmei , Tang, Xiumei , Tayyab, Muhammad et al. Sugarcane-Peanut Intercropping System Enhances Bacteria Abundance, Diversity, and Sugarcane Parameters in Rhizospheric and Bulk Soils . | FRONTIERS IN MICROBIOLOGY , 2022 , 12 . |
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Background: Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1/P-gp) is a major cause of cancer chemotherapy failure, but the regulation mechanisms are largely unknown. Methods: Based on single gene knockout, we studied the regulation of CDK6-PI3K axis on ABCB1-mediated MDR in human cancer cells. CRISPR/Cas9 technique was performed in KB-C2 cells to knockout cdk6 or cdk4 gene. Western blot, RT-PCR and transcriptome analysis were performed to investigate target gene deletion and expression of critical signaling factors. The effect of cdk4 or cdk6 deficiency on cell apoptosis and the cell cycle was analyzed using flow cytometry. In vivo studies were performed to study the sensitivity of KB-C2 tumors to doxorubicin, tumor growth and metastasis. Results: Deficiency of cdk6 led to remarkable downregulation of ABCB1 expression and reversal of ABCB1-mediated MDR. Transcriptomic analysis revealed that CDK6 knockout regulated a series of signaling factors, among them, PI3K 110 alpha and 110 beta, KRAS and MAPK10 were downregulated, and FOS-promoting cell autophagy and CXCL1-regulating multiple factors were upregulated. Notably, PI3K 110 alpha/110 beta deficiency in-return downregulated CDK6 and the CDK6-PI3K axis synergizes in regulating ABCB1 expression, which strengthened the regulation of ABCB1 over single regulation by either CDK6 or PI3K 110 alpha/110 beta. High frequency of alternative splicing (AS) of premature ABCB1 mRNA induced by CDK6, CDK4 or PI3K 110 alpha/110 beta level change was confirmed to alter the ABCB1 level, among them 10 common skipped exon (SE) events were found. In vivo experiments demonstrated that loss of cdk6 remarkably increased the sensitivity of KB-C2 tumors to doxorubicin by increasing drug accumulation of the tumors, resulting in remarkable inhibition of tumor growth and metastasis, as well as KB-C2 survival in the nude mice. Conclusions: CDK6-PI3K as a new target signaling axis to reverse ABCB1-mediated MDR is reported for the first time in cancers. Pathways leading to inhibition of cancer cell proliferation were revealed to be accompanied by CDK6 deficiency.
Keyword ：
ATP-binding cassette (ABC) transporter ABCB1/P-gp ATP-binding cassette (ABC) transporter ABCB1/P-gp cancer cancer Cyclin dependent kinase 6 (CDK6) Cyclin dependent kinase 6 (CDK6) Multidrug resistance (MDR) Multidrug resistance (MDR) PI3K 110 alpha/110 beta PI3K 110 alpha/110 beta Signaling axis Signaling axis Transcriptome sequencing Transcriptome sequencing
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| GB/T 7714 | Zhang, Lei , Li, Yidong , Hu, Chaohua et al. CDK6-PI3K signaling axis is an efficient target for attenuating ABCB1/P-gp mediated multi-drug resistance (MDR) in cancer cells [J]. | MOLECULAR CANCER , 2022 , 21 (1) . |
| MLA | Zhang, Lei et al. "CDK6-PI3K signaling axis is an efficient target for attenuating ABCB1/P-gp mediated multi-drug resistance (MDR) in cancer cells" . | MOLECULAR CANCER 21 . 1 (2022) . |
| APA | Zhang, Lei , Li, Yidong , Hu, Chaohua , Chen, Yangmin , Chen, Zhuo , Chen, Zhe-Sheng et al. CDK6-PI3K signaling axis is an efficient target for attenuating ABCB1/P-gp mediated multi-drug resistance (MDR) in cancer cells . | MOLECULAR CANCER , 2022 , 21 (1) . |
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Metabolic composition can have potential impact on several vital agronomic traits, and metabolomics, which represents the bioactive compounds in plant tissues, is widely considered as a powerful approach for linking phenotype-genotype interactions. However, metabolites related to cane traits such as sugar content, rind color, and texture differences in different sugarcane cultivars using metabolome integrated with transcriptome remain largely inconclusive. In this study, metabolome integrated with transcriptome analyses were performed to identify and quantify metabolites composition, and have better insight into the molecular mechanisms underpinning the different cane traits, namely, brix, rind color, and textures in the stems (S) and leaves (L) of sugarcane varieties FN41 and 165402. We also identified metabolites and associated genes in the phenylpropanoid and flavonoid biosynthesis pathways, starch and sucrose metabolism. A total of 512 metabolites from 11 classes, with the vast majority (122) belonging to flavonoids were identified. Moreover, the relatively high amount of D-fructose 6-p, D-glucose6-p and glucose1-p detected in FN41L may have been transported and distributed by source and sink of the cane, and a majority of them reached the stem of sugarcane FN41L, thereby promoting the high accumulation of sugar in FN41S. Observations also revealed that genes such as C4H, CHS, F3H, F3'H, DFR, and FG2 in phenylpropanoid and flavonoid biosynthesis pathways were the major factors impacting the rind color and contrasting texture of FN41 and 165204. Further analysis revealed that weighted gene co-expression network analysis (WGCNA) hub genes and six transcription factors, namely, Tify and NAC, MYB-related, C2C2-Dof, WRKY, and bHLH play a key role in phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, starch and sucrose metabolism. Additionally, metabolites such as L-phenylalanine, tyrosine, sinapaldehyde, pinobanksin, kaempferin, and nictoflorin were the potential drivers of phenotypic differences. Our finding also demonstrated that genes and metabolites in the starch and sucrose metabolism had a significant effect on cane sugar content. Overall, this study provided valuable insight into the molecular mechanisms underpinning high sugar accumulation and rind color in sugarcane, which we believe is important for future sugarcane breeding programs and the selection of high biomass varieties.
Keyword ：
flavonoids flavonoids metabolome and transcriptome metabolome and transcriptome sugarcane sugarcane sugar metabolism sugar metabolism WGCNA WGCNA
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| GB/T 7714 | Yuan, Zhaonian , Dong, Fei , Pang, Ziqin et al. Integrated Metabolomics and Transcriptome Analyses Unveil Pathways Involved in Sugar Content and Rind Color of Two Sugarcane Varieties [J]. | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
| MLA | Yuan, Zhaonian et al. "Integrated Metabolomics and Transcriptome Analyses Unveil Pathways Involved in Sugar Content and Rind Color of Two Sugarcane Varieties" . | FRONTIERS IN PLANT SCIENCE 13 (2022) . |
| APA | Yuan, Zhaonian , Dong, Fei , Pang, Ziqin , Fallah, Nyumah , Zhou, Yongmei , Li, Zhi et al. Integrated Metabolomics and Transcriptome Analyses Unveil Pathways Involved in Sugar Content and Rind Color of Two Sugarcane Varieties . | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
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Background: As one of the vital crops globally, sugarcane (Saccharum officinarum L.) has been one of model crops for conducting metabolome research. Although many studies have focused on understanding bioactive components in specific sugarcane tissues, crucial questions have been left unanswered about the response of metabolites to niche differentiation such as different sugarcane tissues (leaf, stem and root), and soil regions (rhizosphere and bulk) under silicon (Si) amended soils. Here, nontargeted metabolite profiling method was leveraged to assess the similarities and differences in the abundance and community composition of metabolites in the different sugarcane and soil compartments. Identify the compartment-specific expression patterns of metabolites, and their association with cane agronomic traits and edaphic factors. We also investigated the response of sugarcane agronomic traits and edaphic factors to Si amended soil. Results: We found that Si fertilizer exhibited the advantages of overwhelmingly promoting the height and theoretical production of cane, and profoundly increased soil Si content by 24.8 and 27.0%, while soil available potassium (AK) was enhanced by 3.07 and 2.67 folds in the bulk and rhizosphere soils, respectively. It was also noticed that available phosphorus (AP) in the rhizosphere soil tremendously increased by 105.5%. We detected 339 metabolites in 30 samples using LC-MS/MS analyses, 161 of which were classified and annotated, including organooxygen compounds (19.9%), carboxylic acids and derivatives (15.5%), fatty acyls (15.5%), flavonoids (4.4%), phenols (4.4%), and benzene and substituted derivatives (3.7%). In addition, the total percentages covered by these core metabolites in each compartment ranged from 94.0% (bulk soil) to 93.4% (rhizosphere soil), followed by 87.4% (leaf), 81.0% (root) and 80.5% (stem), suggesting that these bioactive compounds may have migrated from the belowground tissues and gradually filtered in various aboveground niches of the plant. We also observed that the variations and enrichment of metabolites abundance and community were compartment-specific. Furthermore, some key bioactive compounds were markedly associated with plant growth parameters and soil edaphic. Conclusion: Taken together, we hypothesized that Si utilization can exhibit the advantage of enhancing edaphic factors and cane agronomic traits, and variations in metabolites community are tissue-specific.
Keyword ：
Edaphic factors Edaphic factors Metabolites Metabolites Silicon fertilizer Silicon fertilizer Sugarcane agronomic traits Sugarcane agronomic traits Sugarcane and soil compartments Sugarcane and soil compartments
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| GB/T 7714 | Fallah, Nyumah , Pang, Ziqin , Dong, Fei et al. Niche differentiation modulates metabolites abundance and composition in silicon fertilizer amended soil during sugarcane growth [J]. | BMC PLANT BIOLOGY , 2022 , 22 (1) . |
| MLA | Fallah, Nyumah et al. "Niche differentiation modulates metabolites abundance and composition in silicon fertilizer amended soil during sugarcane growth" . | BMC PLANT BIOLOGY 22 . 1 (2022) . |
| APA | Fallah, Nyumah , Pang, Ziqin , Dong, Fei , Zhou, Yongmei , Lin, Wenxiong , Fabrice, Kabore Manegdebwaoga Arthur et al. Niche differentiation modulates metabolites abundance and composition in silicon fertilizer amended soil during sugarcane growth . | BMC PLANT BIOLOGY , 2022 , 22 (1) . |
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