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学者姓名：傅华英
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Abstract ：
Plants have developed various resistance mechanisms against herbivorous insects through prolonged coevolution. Plant defence responses can be triggered by specific compounds present in insect saliva. Apyrase, a known enzyme that catalyzes the hydrolysis of adenosine triphosphate (ATP) and adenosine diphosphate (ADP) into adenosine monophosphate (AMP) and inorganic phosphorus, has recently been identified in some herbivorous insects. However, whether insect salivary apyrase induces or inhibits plant responses remains poorly understood. In this study, we identified an apyrase-like protein in the salivary proteome of the fall armyworm, Spodoptera frugiperda, named Sfapyrase. Sfapyrase was primarily expressed in the salivary gland and secreted into plants during insect feeding. Transient expression of Sfapyrase in tobacco and maize enhanced plant resistance and resulted in decreased insect feeding. Knockdown of Sfapyrase through RNA interference led to increased growth and feeding of S. frugiperda. Furthermore, we showed that Sfapyrase activates the jasmonic acid signalling pathway and promotes the synthesis of secondary metabolites, especially benzoxazinoids, thereby enhancing resistance to S. frugiperda. In summary, our findings demonstrated that Sfapyrase acts as a salivary elicitor, inducing maize jasmonic acid defence responses and the production of insect-resistant benzoxazinoids. This study provides valuable insights into plant-insect interactions and offers potential targets for developing innovative insect pest management strategies.
Keyword ：
apyrase apyrase benzoxazinoids benzoxazinoids jasmonic acid jasmonic acid plant defence plant defence salivary elicitor salivary elicitor Spodoptera frugiperda Spodoptera frugiperda
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| GB/T 7714 | Yao, Yang , Lin, Huan-tai , Chen, Yao-hui et al. Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (1) : 406-420 . |
| MLA | Yao, Yang et al. "Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response" . | PLANT CELL AND ENVIRONMENT 48 . 1 (2025) : 406-420 . |
| APA | Yao, Yang , Lin, Huan-tai , Chen, Yao-hui , Chen, Li-lan , Zhang, Hui-li , Fu, Hua-ying et al. Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (1) , 406-420 . |
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本发明公开了一株甘蔗内生粘质沙雷氏菌及其应用，属于农业微生物技术领域。所述甘蔗内生粘质沙雷氏菌分类命名为粘质沙雷氏菌（Serratia marcescens）SM，已于2023年10月18日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20231923，保藏地址为湖北省武汉市武昌区八一路299号。所述粘质沙雷氏菌从甘蔗茎中分离的，其活体可以作为以粘质沙雷氏菌活体或其代谢产物具有以下作用：（1）对甘蔗上的粘虫幼虫具有杀虫作用；（2）对于甘蔗白条病和甘蔗梢腐病病菌具有抗菌作用；（3）具有促进甘蔗发芽生长的作用。
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| GB/T 7714 | 王锦达 , 林东江 , 高三基 et al. 一株甘蔗内生粘质沙雷氏菌及其应用 : CN202410055646.3[P]. | 2024-01-15 . |
| MLA | 王锦达 et al. "一株甘蔗内生粘质沙雷氏菌及其应用" : CN202410055646.3. | 2024-01-15 . |
| APA | 王锦达 , 林东江 , 高三基 , 张慧丽 , 傅华英 , 黄美婷 . 一株甘蔗内生粘质沙雷氏菌及其应用 : CN202410055646.3. | 2024-01-15 . |
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Sugarcane rust diseases are caused by Puccinia melanocephala (brown rust) and Puccinia kuehnii (orange rust), and significantly threaten the sustainable and stable development of the global sugarcane industry. Erianthus arundinaceus within the Saccharum complex is a potential germplasm resource for sugarcane breeding and is characterized by its tolerance of infertile land, drought, and diseases. However, the research on resistance to rust in E. arundinaceus clones and their offspring (F1 and backcross with modern sugarcane varieties) is limited. In this study, a total of 201 leaf samples from Saccharum spp. hybrids with rust symptoms were collected and screened for disease occurrence. PCR detection revealed that 17.9% and 34.8% of the samples were infected by P. melanocephala and P. kuehnii, respectively. Additionally, 12.9% of the samples were infected by both pathogens. A total of 88 clones of E. arundinaceus offspring and the parents plus 3 additional E. arundinaceus were selected for the identification of brown rust resistance by an artificial inoculation method. Among them, 61 clones displayed high resistance to brown rust. Molecular detection showed that 13 offspring of E. arundinaceus and 6 backcross parents of the "ROC" series exhibited the major resistance gene (Bru1) for brown rust. Unexpectedly, the Bru1 gene was absent in 42 clones that were resistant to brown rust, suggesting that other resistance genes for brown rust likely exist in E. arundinaceus and their offspring. Our results offer some significant genetic resources for developing sugarcane cultivars with resistance against rust.
Keyword ：
Bru1 gene Bru1 gene Erianthus arundinaceus Erianthus arundinaceus germplasm resources germplasm resources Saccharum spp. hybrids Saccharum spp. hybrids sugarcane rust sugarcane rust
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| GB/T 7714 | Chen, Jun-Lv , Sun, Sheng-Ren , Wang, Zhu-Qing et al. Identification of Causal Agents of Rust of Saccharum spp. and Assessment of Resistance to Brown Rust in Erianthus arundinaceus Clones and Their Offspring [J]. | PLANTS-BASEL , 2025 , 14 (8) . |
| MLA | Chen, Jun-Lv et al. "Identification of Causal Agents of Rust of Saccharum spp. and Assessment of Resistance to Brown Rust in Erianthus arundinaceus Clones and Their Offspring" . | PLANTS-BASEL 14 . 8 (2025) . |
| APA | Chen, Jun-Lv , Sun, Sheng-Ren , Wang, Zhu-Qing , Fu, Hua-Ying , Xu, Huan-Yin , Chang, Hai-Long et al. Identification of Causal Agents of Rust of Saccharum spp. and Assessment of Resistance to Brown Rust in Erianthus arundinaceus Clones and Their Offspring . | PLANTS-BASEL , 2025 , 14 (8) . |
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Sugarcane (Saccharum spp.) is an important sugar and biofuel crop in the world. It is frequently subjected to drought stress, thus causing considerable economic losses. Transgenic technology is an effective breeding approach to improve sugarcane tolerance to drought using drought-inducible promoter(s) to activate drought-resistance gene(s). In this study, six different promoters were cloned from sugarcane bacilliform virus (SCBV) genotypes exhibiting high genetic diversity. In beta-glucuronidase (GUS) assays, expression of one of these promoters (PSCBV-YZ2060) is similar to the one driven by the CaMV 35S promoter and >90% higher compared to the other cloned promoters and Ubi1. Three SCBV promoters (PSCBV-YZ2060, PSCBV-TX, and PSCBV-CHN2) function as drought-induced promoters in transgenic Arabidopsis plants. In Arabidopsis, GUS activity driven by promoter PSCBV-YZ2060 is also upregulated by abscisic acid (ABA) and is 2.2-5.5-fold higher when compared to the same activity of two plant native promoters (PScRD29A from sugarcane and PAtRD29A from Arabidopsis). Mutation analysis revealed that a putative promoter region 1 (PPR1) and two ABA response elements (ABREs) are required in promoter PSCBV-YZ2060 to confer drought stress response and ABA induction. Yeast one-hybrid and electrophoretic mobility shift assays uncovered that transcription factors ScbZIP72 from sugarcane and AREB1 from Arabidopsis bind with two ABREs of promoter PSCBV-YZ2060. After ABA treatment or drought stress, the expression levels of endogenous ScbZIP72 and heterologous GUS are significantly increased in PSCBV-YZ2060:GUS transgenic sugarcane plants. Consequently, promoter PSCBV-YZ2060 is a possible alternative promoter for genetic engineering of drought-resistant transgenic crops such as sugarcane.
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| GB/T 7714 | Sun, Sheng-Ren , Wu, Xiao-Bin , Chen, Jian-Sheng et al. Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants [J]. | COMMUNICATIONS BIOLOGY , 2024 , 7 (1) . |
| MLA | Sun, Sheng-Ren et al. "Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants" . | COMMUNICATIONS BIOLOGY 7 . 1 (2024) . |
| APA | Sun, Sheng-Ren , Wu, Xiao-Bin , Chen, Jian-Sheng , Huang, Mei-Ting , Fu, Hua-Ying , Wang, Qin-Nan et al. Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants . | COMMUNICATIONS BIOLOGY , 2024 , 7 (1) . |
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Objectives The pathogen of Pantoea stewartii (Ps) is the causal agent of bacterial disease in corn and various graminaceous plants. Ps has two subspecies, Pantoea stewartii subsp. stewartia (Pss) and Pantoea stewartii subsp. indologenes (Psi). This study presents two complete genomes of Ps strains including ATCC 8199 isolated from maize and PSCN1 causing bacterial wilt in sugarcane. The two bacterial genomes information will be helpful for taxonomy analysis in this genus Pantoea at whole-genome levels and accurately discriminated the two subspecies of Pss and Psi. Data description The reference strain ATCC 8199 isolated from maize was purchased from Beijing Biobw Biotechnology Co., Ltd. (China) and the strain of PSCN1 was isolated from sugarcane cultivar YZ08-1095 in Zhanjiang, Guangdong province of China. Two complete genomes were sequenced using Illumina Hiseq (second-generation) and Oxford Nanopore (third-generation) platforms. The genome of the strain ATCC 8199 comprised of 4.78 Mb with an average GC content of 54.03%, along with five plasmids, encoding a total of 4,846 gene with an average gene length of 827 bp. The genome of PSCN1 comprised of 5.03 Mb with an average GC content of 53.78%, along with two plasmids, encoding a total of 4,725 gene with an average gene length of 913 bp. The bacterial pan-genome analysis highlighted the strain ATCC 8199 was clustered into a subgroup with a Pss strain CCUG 26,359 from USA, while the strain PSCN1 was clustered into another subgroup with a Ps strain NRRLB-133 from USA. These findings will serve as a useful resource for further analyses of the evolution of Ps strains and corresponding disease epidemiology worldwide.
Keyword ：
Complete genome Complete genome Pantoea stewartia Pantoea stewartia Stewart's bacterial wilt Stewart's bacterial wilt Sugarcane (Saccharum spp.) Sugarcane (Saccharum spp.)
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| GB/T 7714 | Chu, Na , Liu, Tian-Tian , Zhang, Hui-Li et al. Complete genome sequences of two Pantoea stewartii strains ATCC 8199 from maize and PSCN1 from sugarcane [J]. | BMC GENOMIC DATA , 2024 , 25 (1) . |
| MLA | Chu, Na et al. "Complete genome sequences of two Pantoea stewartii strains ATCC 8199 from maize and PSCN1 from sugarcane" . | BMC GENOMIC DATA 25 . 1 (2024) . |
| APA | Chu, Na , Liu, Tian-Tian , Zhang, Hui-Li , Cui, Dong , Huang, Mei-Ting , Fu, Hua-Ying et al. Complete genome sequences of two Pantoea stewartii strains ATCC 8199 from maize and PSCN1 from sugarcane . | BMC GENOMIC DATA , 2024 , 25 (1) . |
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Plants must adapt to the complex effects of several stressors brought on by global warming, which may result in interaction and superposition effects between diverse stressors. Few reports are available on how drought stress affects Xanthomonas albilineans (Xa) infection in sugarcane (Saccharum spp. hybrids). Drought and leaf scald resistance were identified on 16 sugarcane cultivars using Xa inoculation and soil drought treatments, respectively. Subsequently, four cultivars contrasting to drought and leaf scald resistance were used to explore the mechanisms of drought affecting Xa-sugarcane interaction. Drought stress significantly increased the occurrence of leaf scald and Xa populations in susceptible cultivars but had no obvious effect on resistant cultivars. The ROS bursting and scavenging system was significantly activated in sugarcane in the process of Xa infection, particularly in the resistant cultivars. Compared with Xa infection alone, defense response via the ROS generating and scavenging system was obviously weakened in sugarcane (especially in susceptible cultivars) under Xa infection plus drought stress. Collectively, ROS might play a crucial role involving sugarcane defense against combined effects of Xa infection and drought stress.
Keyword ：
defense response defense response drought drought leaf scald leaf scald reactive oxygen species reactive oxygen species Saccharum spp. hybrids Saccharum spp. hybrids
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| GB/T 7714 | Wei, Yao-Sheng , Zhao, Jian-Ying , Javed, Talha et al. Insights into Reactive Oxygen Species Production-Scavenging System Involved in Sugarcane Response to Xanthomonas albilineans Infection under Drought Stress [J]. | PLANTS-BASEL , 2024 , 13 (6) . |
| MLA | Wei, Yao-Sheng et al. "Insights into Reactive Oxygen Species Production-Scavenging System Involved in Sugarcane Response to Xanthomonas albilineans Infection under Drought Stress" . | PLANTS-BASEL 13 . 6 (2024) . |
| APA | Wei, Yao-Sheng , Zhao, Jian-Ying , Javed, Talha , Ali, Ahmad , Huang, Mei-Ting , Fu, Hua-Ying et al. Insights into Reactive Oxygen Species Production-Scavenging System Involved in Sugarcane Response to Xanthomonas albilineans Infection under Drought Stress . | PLANTS-BASEL , 2024 , 13 (6) . |
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The plant endophytic bacteria are a great source of nature insecticides. However, no such endophytic bacteria have been found in sugarcane, to address this gap, we isolated and identified a strain of Serratia marcescens with moderate insecticidal activity from sugarcane. Taken armyworm Mythimna separata as example, the mortality rates of oral infection and injection infection were 47.06 % and 91 %, respectively. The SM has significant negative affect on the growth, development, and reproduction of M. separata. After determining that these insecticidal substances, 33 potential virulence proteins were screened through the identification and prediction of bacterial proteins. Later we confirmed serralysin was a vital toxic protein from SM that caused M. separata death by prokaryotic expression. In addition, we also found that the intestinal tissue cells infected with SM or serralysin were severely diseased, which may be a major factor in M. separata demise. Finally, through gene expression level, protein molecular docking, we found the aminopeptidase-N would be one of the potential receptors of serralysin. Taken together, our findings indicate that sugarcane endophyte S. marcescens may be beneficial for pest control in sugarcane and explain its insecticidal mechanism. This study provides new ideas and materials for the biological control of pests.
Keyword ：
Mythimna separata (Walker) Mythimna separata (Walker) Serralysin Serralysin Serratia marcescens Serratia marcescens
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| GB/T 7714 | Lin, Dong-Jiang , Zhou, Jiang-xiong , Ali, Ahmad et al. Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker) [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 262 . |
| MLA | Lin, Dong-Jiang et al. "Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker)" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 262 (2024) . |
| APA | Lin, Dong-Jiang , Zhou, Jiang-xiong , Ali, Ahmad , Fu, Hua-ying , Gao, San-ji , Jin, Lin et al. Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker) . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 262 . |
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本发明公开了一株沙福芽孢杆菌（Bacillus safensis）AS2505及其应用，属于微生物技术领域。所述沙福芽孢杆菌（Bacillus safensis）AS2505已于2023年10月18日保藏于中国典型培养物保藏中心，保藏地址为湖北武汉市武昌区八一路珞珈山武汉大学，保藏编号为CCTCC NO：M 20231924。所述沙福芽孢杆菌（Bacillus safensis）AS2505可产胞外蛋白酶、纤维素酶。所述沙福芽孢杆菌（Bacillus safensis）AS2505特异性拮抗甘蔗白条病菌，且对不同致病力的甘蔗白条病菌菌株均有抑制作用，可显著降低甘蔗白条病的病情指数。同时，所述沙福芽孢杆菌（Bacillus safensis）AS2505能显著促进甘蔗幼苗的生长，在甘蔗生产中具有较好的应用潜力。
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| GB/T 7714 | 张慧丽 , 卞润恬 , 高三基 et al. 一株沙福芽孢杆菌Bacillus safensis AS2505及其应用 : CN202311785671.9[P]. | 2023-12-25 . |
| MLA | 张慧丽 et al. "一株沙福芽孢杆菌Bacillus safensis AS2505及其应用" : CN202311785671.9. | 2023-12-25 . |
| APA | 张慧丽 , 卞润恬 , 高三基 , 尤哲扬 , 傅华英 , 王锦达 . 一株沙福芽孢杆菌Bacillus safensis AS2505及其应用 : CN202311785671.9. | 2023-12-25 . |
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宿根矮化病Ratoon stunting diseases(RSD)是由木质部限制性赖氏细菌木质部变种Leifsonia xyli subsp.Xyli(Lxx)引起的一种主要甘蔗细菌性病害之一,在世界各甘蔗产区普遍发生。为明确我国主要蔗区RSD发生率和收获方式对RSD发生率的影响,本文从广东和福建的罹病甘蔗蔗茎样品成功分离获得病原细菌,基于细菌16S核糖体和16-23S核糖体间隔序列(ITS)证实为Lxx。随后,从广西、广东和福建主要蔗区采集甘蔗品种蔗茎样品1446份,以靶标16-23S核糖体ITS区域的Lxx-F/Lxx-R为检测引物,开展RSD分子检测。结果表明,福建蔗区RSD检出率较低,为0～9.4%,广西、广东蔗区RSD检出率分别为20.4～85.2%和82.1%,尤其是广西龙州县和广东遂溪县蔗区,RSD检出率高达80%以上。在机械收获和人工砍刀收获方式下的宿根蔗蔗茎样品RSD检出率没有显著差异。本研究结果为我国蔗区RSD的科学防控提供依据。
Keyword ：
PCR检测 PCR检测 宿根矮化病 宿根矮化病 木质部限制性赖氏细菌木质部变种 木质部限制性赖氏细菌木质部变种 检出率 检出率 甘蔗 甘蔗
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| GB/T 7714 | 尉耀升 , 许珲镁 , 呼忠婷 et al. 我国华南蔗区甘蔗宿根矮化病的PCR分子检测 [C] //第二十届中国作物学会学术年会 . 2023 . |
| MLA | 尉耀升 et al. "我国华南蔗区甘蔗宿根矮化病的PCR分子检测" 第二十届中国作物学会学术年会 . (2023) . |
| APA | 尉耀升 , 许珲镁 , 呼忠婷 , 刘婷 , 刘玮婷 , 高三基 et al. 我国华南蔗区甘蔗宿根矮化病的PCR分子检测 第二十届中国作物学会学术年会 . (2023) . |
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PR10是一种病程相关蛋白(pathogenesis-relatedprotein,PR),在植物生长发育、应激生物和非生物胁迫时发挥重要作用。本研究利用RT-PCR技术从甘蔗赤条病抗病品种新台糖22号和感病品种闽糖11-610叶片克隆获得Sc PR10基因,并利用实时荧光定量PCR(RT-q PCR)、转录组和蛋白质组分析,研究在接种燕麦食酸菌燕麦亚种(Acidovorax avenae subsp. avenae, Aaa)之后这2个品种Sc PR10基因的转录和蛋白表达模式。序列分析显示,本研究克隆获得2个Sc PR10基因,编码187个氨基酸,比其他植物PR10蛋白多了27个氨基酸,含有保守结构域P-loop和Betv1;与已报道的甘蔗、高粱和斑茅的PR10亲缘关系较近。在Aaa胁迫下,RT-q PCR分析表明抗病、感病品种Sc PR10基因的表达量均显著上调,尤其在接种24 h时,表达量最高,分别为对照的27.2倍和39.7倍;转录组数据分析结果显示,该基因表达水平在抗病、感病品种上均显著提高,尤其在接种72h时,表达量最高,log2FC值为5.3～5.4。蛋白互作预测发现,Sc PR10与植物PDR型ABCG转运蛋白(Cluster-13677.282407)、蛋白激酶(Cluster-13677.166559)之间存在互作关系。蛋白质组数据分析显示,在Aaa接种24h时,抗病、感病品种Sc PR10均为上调表达, log2FC值为1.65～1.69;与Sc PR10互作的PDR型ABCG转运蛋白成员的表达量在抗病、感病品种上有不同程度提高;蛋白激酶表达量在感病品种上有显著提高,但是,在抗病品种上表达量没有显著变化。本研究结果表明,Sc PR10可能与PDR型ABCG转运蛋白正向协同参与甘蔗寄主应答Aaa病菌侵染的防御响应。
Keyword ：
PR10蛋白 PR10蛋白 基因表达 基因表达 燕麦食酸菌燕麦亚种 燕麦食酸菌燕麦亚种 甘蔗 甘蔗 生物胁迫 生物胁迫
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| GB/T 7714 | 李娟 , 周敬如 , 储娜 et al. 甘蔗ScPR10基因的克隆及其响应赤条病菌侵染的表达特征分析 [J]. | 作物学报 , 2023 , 49 (01) : 97-104 . |
| MLA | 李娟 et al. "甘蔗ScPR10基因的克隆及其响应赤条病菌侵染的表达特征分析" . | 作物学报 49 . 01 (2023) : 97-104 . |
| APA | 李娟 , 周敬如 , 储娜 , 孙会东 , 黄美婷 , 傅华英 et al. 甘蔗ScPR10基因的克隆及其响应赤条病菌侵染的表达特征分析 . | 作物学报 , 2023 , 49 (01) , 97-104 . |
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