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学者姓名：陈新华
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Abstract ：
Pseudomonas plecoglossicida is a pathogen bacterium responsible for visceral white spot disease (VWND) in Large Yellow Croakers (Larimichthys crocea), causing significant economic losses in commercial fish farms. The prolinealanine-alanine-arginine repeats protein (PAAR) is a core component of the spike structure in the type VI secretion system (T6SS), which injects toxic effectors into host and contributes to bacterial virulence. However, the role of the PAAR gene in P. plecoglossicida and its impact on bacterial infection and host immune responses remain unexplored. In this study, PAAR-1 was identified for the first time in P. plecoglossicida as an effector gene within the T6SS-1 gene cluster, which is regulated and secreted by T6SS-1. The P. plecoglossicida mutant strain (z.PAAR-1) and its complementary strain (C-z.PAAR-1) were constructed for subsequent investigation. Compared to the wild-type strain, z.PAAR-1 exhibited reduced biofilm formation, adhesion, total antioxidant capacity, and secretion of T6SS core protein Hcp-1. In vitro, z.PAAR-1 showed decreased survival rates in Large Yellow Croaker macrophage cell line (LYC-FM) due to impaired oxidative stress tolerance. In vivo, infection with z.PAAR-1 led to a significant reduction in mortality, bacterial colonization, and the formation of spleen nodules in Large Yellow Croakers. Comparative transcriptome analysis revealed that PAAR-1 predominantly influences the host Toll-like receptor (TLR) signaling pathway and apoptosis by upregulating the expression of plasma membrane-associated TLRs, including TLR1, TLR2, and TLR5, while downregulating the expression of endosomal TLRs like TLR3, TLR7, TLR8, and TLR9, along with its downstream molecules such as MyD88 and TRAF3. Additionally, knockout PAAR1 downregulates apoptosis-related genes including AP-1, NF-kappa B, FAS-L TNF alpha, Caspase8, and FAS-L. Real-time quantitative polymerase chain reaction (RT-qPCR) further confirmed these findings. Furthermore, the proportion of apoptotic cells was significantly lower in the z.PAAR-1 infected LYC-PKM cells. These results indicate that PAAR-1 is involved in regulating TLR signaling pathway and apoptosis in Large Yellow Croaker. This study provides the first identification of the core T6SS-1 gene PAAR-1 in P. plecoglossicida, offering valuable insights into its pathogenic mechanisms and presenting a potential target for attenuated vaccine development.
Keyword ：
Immune response Immune response Large yellow croaker Large yellow croaker PAAR-1 PAAR-1 Pathogenicity Pathogenicity Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Zhang, Baoyu , Li, Youshen , Li, Jianxin et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) [J]. | AQUACULTURE , 2026 , 610 . |
| MLA | Zhang, Baoyu et al. "Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea)" . | AQUACULTURE 610 (2026) . |
| APA | Zhang, Baoyu , Li, Youshen , Li, Jianxin , Zhai, Yu , Meng, Ziyu , Huang, Xiyue et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) . | AQUACULTURE , 2026 , 610 . |
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Sea cucumber skin ulceration syndrome (SUS) is associated with a diverse range of bacterial and parasitic pathogens. Nonetheless, research efforts aimed at identifying novel pathogens and developing effective prevention and control strategies remain limited. In this study, we identified a strain of Photobacterium damselae and designated it as P. damselae sea cucumber xiapu (P. damselae SCXP), recognizing it as a new pathogenic bacterium associated with SUS in Apostichopus japonicus. We developed a detection method using a TaqMan probebased qPCR assay specific to P. damselae SCXP, which supports the rapid diagnosis of this pathogen. Additionally, artificial infection experiments confirmed that P. damselae SCXP facilitates the progression of SUS in Apostichopus japonicus. Drug sensitivity analysis revealed that the strain was highly sensitive to 16 antibiotics (e. g., novobiocin, ceftazidime, ceftriaxone) with inhibition zone diameters >= 20 mm, providing a reference for aquatic drug use. Furthermore, transcriptomic analyses were conducted on the coelomocytes of Apostichopus japonicus infected with P. damselae SCXP. The KEGG enrichment analysis identified distinct signaling pathways that were differentially expressed in the P. damselae SCXP-infected groups at 6, 12, and 24 h post-infection. At the 6-h mark, the focal adhesion pathway exhibited the highest number of differentially expressed genes (DEGs), followed by the PI3K-Akt, mTOR, and MAPK signaling pathways. At 12 h post-infection, the complement and coagulation cascades showed the highest number of DEGs, followed by phagosome-related pathways. By 24 h, the proteasome and lysosome pathways demonstrated an increased number of DEGs. Venn diagram analysis revealed that P. damselae SCXP infection suppressed the expression of several genes, including deoxyribonuclease-1 (DNase I) and acid-sensing ion channel 1-like protein (ASIC1), as validated by qRT-PCR. This study constitutes the first comprehensive investigation into the transcriptomic profile of Apostichopus japonicus coelomocytes in response to P. damselae SCXP infection. Future research endeavors should concentrate on elucidating the roles of DNase I and ASIC1 protein, as these elements may have significant implications for the development of targeted prevention and control strategies. Our study offers a theoretical foundation for the prevention and management of P. damselae-induced SUS in sea cucumbers.
Keyword ：
Photobacterium damselae Photobacterium damselae Sea cucumber Apostichopus japonicus Sea cucumber Apostichopus japonicus Skin ulceration syndrome Skin ulceration syndrome Transcriptomic Transcriptomic
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| GB/T 7714 | Yang, Linwei , Liu, Shuming , Lv, Hui et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Yang, Linwei et al. "Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome" . | AQUACULTURE 612 (2026) . |
| APA | Yang, Linwei , Liu, Shuming , Lv, Hui , Wei, Haiyun , Wu, Tong , Lou, Bilian et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome . | AQUACULTURE , 2026 , 612 . |
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This study investigated the effects of supplementation with recombinant peroxiredoxin IV (Prx IV) on the innate immunity and intestinal health in large yellow croaker (Larimichthys crocea). Fish (n = 160) from triple replicates were fed diets containing 0.00 g kg-1 (Prx1), 0.05 g kg-1 (Prx2), 0.10 g kg-1 (Prx3), and 0.15 g kg-1 (Prx4) Prx IV for 8 weeks. Results demonstrated that Prx IV supplementation significantly improved systemic antioxidant capacity, evidenced by elevated activities of superoxide dismutase and catalase, and increased levels of reduced glutathione. Analysis of the head kidney inflammatory response revealed that dietary Prx IV significantly upregulated gene expression of the anti-inflammatory cytokine interleukin-10 and simultaneously down-regulated the expression of pro-inflammatory cytokines (interleukin 2, interleukin 8, and tumor necrosis factor). Furthermore, western blot analysis confirmed that Prx IV administration markedly suppressed nuclear trans-location of nuclear factor-kappa B p65 in the head kidney. Regarding intestinal effects, dietary Prx IV improved intestinal morphology and significantly enhanced the activities of key digestive enzymes (amylase, lipase, and trypsin). Additionally, Prx IV supplementation significantly upregulated the expression of tight junction proteins (claudin 11 and zona occludens 1, ZO-1). Moreover, dietary administration of 0.15 g kg-1 Prx IV significantly altered intestinal microbiota profiles. Specifically, it increased the abundance of Firmicutes, decreased the abundance of potential pathogens such as Haemophilus parainfluenzae and Klebsiella variicola, and increased the abundance of Bacteroides thetaiotaomicron, a bacterium associated with polysaccharide digestion and absorption. In conclusion, dietary administration of 0.15 g kg-1 Prx IV enhances antioxidant and anti-inflammatory capacities in the head kidney and promotes intestinal health in large yellow croaker. Modulation of the intestinal microbiota may contribute to these beneficial effects.
Keyword ：
Innate immunity Innate immunity Intestinal health Intestinal health Larimichthys crocea Larimichthys crocea Peroxiredoxin IV Peroxiredoxin IV
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| GB/T 7714 | Chen, Zhichu , Liu, Qianqian , Zeng, Chao et al. Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Chen, Zhichu et al. "Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles" . | AQUACULTURE 612 (2026) . |
| APA | Chen, Zhichu , Liu, Qianqian , Zeng, Chao , You, Haokun , Hu, Bing , Zhang, Jiaonan et al. Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles . | AQUACULTURE , 2026 , 612 . |
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Bacterial infection represents a major challenge in large yellow croaker farming, while excessive antibiotic usage has exacerbated the problem of antimicrobial resistance. This situation underscores the critical demand for developing antimicrobial peptides to replace conventional antibiotics. In this study, we designed the antimicrobial peptide RAK18, an 18-amino-acid peptide derived from the C-terminal alpha-helical domain of the large yellow croaker transcription factor Pu.1a, along with its variant RAK18-2, which was engineered through targeted residue substitutions. In vitro assays revealed that RAK18 selectively targeted Pseudomonas plecoglossicida and Bacillus subtilis, whereas RAK18-2 exhibited broader-spectrum antimicrobial activity. Both peptides maintained low cytotoxicity and hemolytic activity at bactericidal concentrations and remained stable under varying temperatures and pH conditions, although salt ion conditions influenced their efficacy. Mechanistic studies showed that they bind to lipopolysaccharide (LPS), inducing bacterial membrane disruption and cell death. In vivo, both RAK18 and RAK18-2 improved survival rates in P. plecoglossicida-infected zebrafish, with RAK18-2 showing superior protective effects. These results not only offer a promising treatment option for managing visceral white nodule disease but also provide the first evidence that the C-terminal alpha-helical peptide of Pu.1 exhibits antibacterial activity, thereby expanding the recognized functional spectrum of Pu.1.
Keyword ：
Antimicrobial peptides Antimicrobial peptides Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) Pu.1 Pu.1 Visceral white nodule disease Visceral white nodule disease
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| GB/T 7714 | He, Hongyu , Zhou, Yuanyuan , Wei, Gonghong et al. Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease [J]. | AQUACULTURE , 2026 , 613 . |
| MLA | He, Hongyu et al. "Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease" . | AQUACULTURE 613 (2026) . |
| APA | He, Hongyu , Zhou, Yuanyuan , Wei, Gonghong , Liao, Yining , Xu, Jing , Chen, Xinhua et al. Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease . | AQUACULTURE , 2026 , 613 . |
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Visceral white nodules disease (VWND) caused by Pseudomonas plecoglossicida in large yellow croaker (Larimichthys crocea) leads to high mortality and significant economic losses for commercial fish farms. In this study, an attenuated mutant was created by deleting the deoR gene from the virulent P. plecoglossicida PQLYC4 strain. The Delta deoR mutant strain exhibits the weak virulence and has the potential to be developed into an attenuated vaccine. The Delta deoR strain was administered to large yellow croakers via intraperitoneal injection (IP) or through direct immersion (DI) for 60 min, and the protective efficacy was assessed at 42 days post-initial vaccination (dpiv). Fish immunized with P. plecoglossicida zdeoR via various routes and doses exhibited a high level of protection compared with the PBS mock-immunized group, with a significant reduction in mortality. The Relative Percent Survival (RPS) in vaccinated fish at 14 days post-challenge was 85.2 % to 89.2 % and 82.5 % to 92.5 % for the PI and DI routes, respectively. Increased expression levels of humoral immunity mediators (IgM, IgT, MHC II alpha, and CD4-1) and cellular immunity molecules (MHC I, CD8 alpha, and ZAP70) were observed at 24 h post-challenge in vaccinated groups compared with control group. Moreover, vaccination with the zdeoR mutant effectively suppressed the proliferation of P. plecoglossicida strain PQLYC4 in large yellow croakers regardless of vaccination route. Significantly elevated levels of specific IgM antibodies were observed in fish of each zdeoRimmunized groups, with a notable increase starting at 14 dpiv, reaching peak levels at 42 dpiv, and followed by a gradual decline starting at 56 dpiv. Specific IgM remained much higher than the mock- immunized group at 150 dpiv, indicating a durable immunity conferred by the zdeoR vaccine strain. This was further supported by a high RPS when challenged at 90, 120, and 150 dpiv. Collectively, these findings indicate that the P. plecoglossicida zdeoR mutant shows potential as a live attenuated vaccine against virulent P. plecoglossicida infection.
Keyword ：
deoR deoR Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea ) Pseudomonas plecoglossicida Pseudomonas plecoglossicida Vaccine Vaccine
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| GB/T 7714 | Qin, Pan , Chen, Xingfu , Yang, Jinmei et al. Evaluation of protective efficacy of Pseudomonas plecoglossicida zdeoR as an attenuated vaccine against virulent challenge in large yellow croaker (Larimichthys crocea) [J]. | AQUACULTURE , 2025 , 596 . |
| MLA | Qin, Pan et al. "Evaluation of protective efficacy of Pseudomonas plecoglossicida zdeoR as an attenuated vaccine against virulent challenge in large yellow croaker (Larimichthys crocea)" . | AQUACULTURE 596 (2025) . |
| APA | Qin, Pan , Chen, Xingfu , Yang, Jinmei , Li, Xin , Wu, Tong , Yang, Jingsong et al. Evaluation of protective efficacy of Pseudomonas plecoglossicida zdeoR as an attenuated vaccine against virulent challenge in large yellow croaker (Larimichthys crocea) . | AQUACULTURE , 2025 , 596 . |
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The T cell receptor (TCR) plays a crucial role in antigen recognition and signal transduction during T cell immunity. While the TCR locus has been well characterized in mammals, its knowledge in teleosts remains limited. In this study, we identified the TCR beta locus in large yellow croaker (Larimichthys crocea), an important mariculture species in China, and found 31 V, 2 D, 13 J, and 2 C gene segments. The 2 C gene segments are highly similar in amino acid sequences, and share conserved residues with TCR beta from other species. A consensus recombination signal sequence (RSS) is found to flank the V, D, and J gene segments, with conserved spacer lengths as observed in mammals. The V gene segments are consisted of a leader exon, an intron, and a V beta exon, and could be categorized into fourteen families based on the nucleotide identity. Furthermore, we found that the recombination of V, D, and J gene segments in the TCR beta locus occurred at the genomic DNA level, followed by fusion with the C gene segments at the mRNA level. Additionally, the usage of J gene segments is restricted to their adjacent downstream C gene segments. qRT-PCR analysis showed that the TCR beta was highly expressed in immune organs and was upregulated after PHA treatment. By exploring a previously published RNA-seq dataset, we found that the V gene segments were differentially expressed after P. plecoglossicida infection, suggesting their involvement in T cell immunity. In summary, we characterized the TCR beta locus in large yellow croaker, which would promote the understanding of T cell immunity in teleosts.
Keyword ：
Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) T cells T cells TCR beta locus TCR beta locus V(D)J recombination V(D)J recombination
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| GB/T 7714 | Shi, Yuan , Zhu, Zhuo , Chen, Qiuxuan et al. Identification and annotation of the T cell receptor beta (TCRβ) locus in large yellow croaker (Larimichthys crocea) [J]. | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY , 2025 , 164 . |
| MLA | Shi, Yuan et al. "Identification and annotation of the T cell receptor beta (TCRβ) locus in large yellow croaker (Larimichthys crocea)" . | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 164 (2025) . |
| APA | Shi, Yuan , Zhu, Zhuo , Chen, Qiuxuan , Teng, Yan , Li, Xinran , Chen, Xinhua . Identification and annotation of the T cell receptor beta (TCRβ) locus in large yellow croaker (Larimichthys crocea) . | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY , 2025 , 164 . |
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Antifreeze proteins (AFPs) are crucial for enhancing an organism's ability to adapt to low temperatures, with broad applications in medicine, frozen food storage and processing, aquaculture, and agriculture. The accurate identification of AFPs is a prerequisite for their utilization, but the high structural and sequence heterogeneity among different AFPs has made their identification a challenging task. To improve AFP prediction performance and enable classification of different AFP types, we propose a novel deep learning-based prediction model, BERT-DomainAFP. Firstly, we integrated existing AFP data using a novel annotation strategy to establish the AntiFreezeDomains dataset. This dataset was then used to train the pre-trained ProteinBERT model, resulting in the development of the BERT-DomainAFP model for AFP prediction. Secondly, for handling of the unbalanced datasets, our BERT-DomainAFP model innovates employing oversampling and undersampling techniques. This ensures the model can fully learn the features of various data types during training and maintain high predictive ability even on more unbalanced datasets. Most importantly, Structural domain annotations are used as training labels, which enables the model to not only accurate predict the presence of AFPs (with an accuracy 98.48%, the highest among existing models), but also classify different types of AFPs based on their unique structural domain features. In summary, our proposed BERT-DomainAFP model not only outperforms existing models in AFP recognition but also supports the classification of different AFP types, offering a promising recognition and classification tool for advancing AFP research and related applications. © 2024, The Authors. All rights reserved.
Keyword ：
Food storage Food storage Prediction models Prediction models
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| GB/T 7714 | Chen, Shengzhen , Zheng, Ping , Zheng, Lele et al. BERT-DomainAFP: Antifreeze Protein Recognition and Classification Model Based on BERT and Structural Domain Annotation [J]. | SSRN , 2025 . |
| MLA | Chen, Shengzhen et al. "BERT-DomainAFP: Antifreeze Protein Recognition and Classification Model Based on BERT and Structural Domain Annotation" . | SSRN (2025) . |
| APA | Chen, Shengzhen , Zheng, Ping , Zheng, Lele , Yao, Qinglong , Meng, Ziyu , Lin, Longshan et al. BERT-DomainAFP: Antifreeze Protein Recognition and Classification Model Based on BERT and Structural Domain Annotation . | SSRN , 2025 . |
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The coagulation system is a mechanism for wound healing after injury, but it also participates in host early immune defense. The coagulation factor VII (FVII) can initiate extrinsic pathway and play an important role in the coagulation process. However, studies of the immune function of FVII are scarce, especially in fish. In this study, we cloned and characterized an FVII-like gene from large yellow croaker (Larimichthys crocea) (LcFVIIL). The open reading frame of LcFVIIL consists of 1437 base pairs and encodes 478 amino acid residues. LcFVIIL contains conserved domains that are present in other vertebrate FVIIs or FVIILs, including a prepropeptide, a gamma-carboxy glutamic acid domain, two epidermal growth factor-like domains, and a serine protease domain. LcFVIIL was highly expressed in the liver and brain, but its expression was low in the other tested tissues. At the cellular level, LcFVIIL was highly expressed in macrophages, and its expression was induced by exposure to Pseudomonas plecoglossicida. We produced the recombinant LcFVIIL light chain (rLcFVIIL-LC), and found that it had obvious antibacterial effects against Gram-positive bacteria but low against Gram-negative bacteria. The rLcFVIIL-LC promoted the proliferation of macrophages. It also significantly induced the expression of proinflammatory factor (IL-1(3 and IL-6) and increased reactive oxygen species activity in large yellow croaker macrophages, while inhibited the expression of anti-inflammatory factor (TGF-(3), suggesting that rLcFVIIL-LC may promote polarization of macrophages towards the M1 type. Taken together, these findings provide insight into the function of fish FVII, and advance our understanding of the role of the coagulation system in host defense.
Keyword ：
Coagulation factor VII Coagulation factor VII Large yellow croaker Large yellow croaker Macrophage Macrophage Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Chen, Yueming , Zhao, Han , Cao, Shuangshuang et al. Molecular characterization of large yellow croaker (Larimichthys crocea) coagulation factor VII-like and its function on macrophage proliferation and polarization [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 158 . |
| MLA | Chen, Yueming et al. "Molecular characterization of large yellow croaker (Larimichthys crocea) coagulation factor VII-like and its function on macrophage proliferation and polarization" . | FISH & SHELLFISH IMMUNOLOGY 158 (2025) . |
| APA | Chen, Yueming , Zhao, Han , Cao, Shuangshuang , Xie, Hongjun , Huang, Jieyu , Chen, Xinhua et al. Molecular characterization of large yellow croaker (Larimichthys crocea) coagulation factor VII-like and its function on macrophage proliferation and polarization . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 158 . |
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Eucommia ulmoides leaf extract (ELE) and Astragalus polysaccharides (APS) have been widely used as immunopotentiators in aquaculture. Our prior research on large yellow croaker (Larimichthys crocea) demonstrated that dietary 1 g/kg APS bolstered fish immunity and antioxidant defense. However, the combined effect of ELE and APS in juvenile large yellow croaker remains unknown. Hence, this study aimed to investigate the synergistic effect of ELE and APS on the growth, antioxidant capacity, and intestinal inflammation in large yellow croaker. A total of 1200 fish were divided into five groups and fed diets with 1 g/kg APS and varying ELE levels: 0 g/kg (ELE0), 0.25 g/kg (ELE0.25), 0.5 g/kg (ELE0.5), 1 g/kg (ELE1), and 2 g/kg (ELE2). After an 8-week feeding period, the ELE0.5 and ELE1 groups showed superior weight gain rate, specific growth rate, and feed efficiency compared to other groups. The ELE1 group also had elevated trypsin and lipase activities in the intestine, whereas alpha-amylase activity was not influenced by ELE addition. Antioxidant enzyme activities, such as hepatopancreas superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the ELE1 group were significantly enhanced, while malondialdehyde (MDA) levels decreased with increasing ELE. Intestinal morphology revealed the highest villi height in proximal and distal intestines of ELE1 group, with no significant change in mucosal thickness. In terms of cytokines, the ELE1 group showed significant down-regulation of pro-inflammatory (tnf-alpha, il-1 beta and il-6) and up-regulation of anti-inflammatory (il-4/13a, il-10 and tgf-beta) markers, modulated by MAPK and mTOR signaling. In conclusion, this study indicates that supplementing diets with 1 g/kg ELE alongside 1 g/kg APS in juvenile large yellow croaker offers the best synergistic effect on fish immunity, including enhanced growth, antioxidant capacity, and relieved intestinal inflammation through MAPK and mTOR signaling.
Keyword ：
Anti-inflammation Anti-inflammation Antioxidant capacity Antioxidant capacity Astragalus polysaccharides Astragalus polysaccharides Eucommia ulmoides leaf extracts Eucommia ulmoides leaf extracts Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea )
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| GB/T 7714 | Shao, Jianchun , Wang, Xuexi , Liu, Qianqian et al. Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
| MLA | Shao, Jianchun et al. "Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 161 (2025) . |
| APA | Shao, Jianchun , Wang, Xuexi , Liu, Qianqian , Lv, Huiyuan , Qi, Qiong , Li, Changhui et al. Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
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Anisarchus medius (Reinhardt, 1837) is a widely distributed Arctic fish, serving as an indicator of climate change impacts on coastal Arctic ecosystems. This study presents a chromosome-level genome assembly for A. medius using PacBio sequencing and Hi-C technology. The PacBio assembly totaled 739.07 Mb across 697 contigs, with a Contig N50 of 10.004 Mb. Hi-C mapping yielded 23 chromosomes, with a successful mapping rate of 90.53% and a Scaffold N50 of 30.20 Mb. Genome BUSCO integrity was 97.05%. Repetitive sequences accounted for 240.83 Mb (32.58%) of the genome. Non-coding RNA annotations included 4,928 rRNAs, 9,663 tRNAs, 347 snRNAs, and 21 snoRNAs. A total of 30,345 protein-coding genes were identified, encoding 46,603 proteins, with a BUSCO completeness of 94.98%. Molecular pathway related to the endocrine system, carbohydrate metabolism, folding, sorting, and degradation, signal transduction, and transport and catabolism contribute to A. medius adaptation to extreme Arctic environments. This high-quality genome provides valuable genetic resources for understanding Arctic adaptations and supporting polar ecological conservation and management.
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| GB/T 7714 | Liu, Ruoyu , Meng, Ziyu , Mu, Yinan et al. Chromosome-level reference genome and annotation of the Arctic fish Anisarchus medius [J]. | SCIENTIFIC DATA , 2025 , 12 (1) . |
| MLA | Liu, Ruoyu et al. "Chromosome-level reference genome and annotation of the Arctic fish Anisarchus medius" . | SCIENTIFIC DATA 12 . 1 (2025) . |
| APA | Liu, Ruoyu , Meng, Ziyu , Mu, Yinan , Zhang, Ran , Ma, Hanhui , Hu, Jingjing et al. Chromosome-level reference genome and annotation of the Arctic fish Anisarchus medius . | SCIENTIFIC DATA , 2025 , 12 (1) . |
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