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学者姓名：陈新华
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Abstract ：
Sea cucumber skin ulceration syndrome (SUS) is associated with a diverse range of bacterial and parasitic pathogens. Nonetheless, research efforts aimed at identifying novel pathogens and developing effective prevention and control strategies remain limited. In this study, we identified a strain of Photobacterium damselae and designated it as P. damselae sea cucumber xiapu (P. damselae SCXP), recognizing it as a new pathogenic bacterium associated with SUS in Apostichopus japonicus. We developed a detection method using a TaqMan probebased qPCR assay specific to P. damselae SCXP, which supports the rapid diagnosis of this pathogen. Additionally, artificial infection experiments confirmed that P. damselae SCXP facilitates the progression of SUS in Apostichopus japonicus. Drug sensitivity analysis revealed that the strain was highly sensitive to 16 antibiotics (e. g., novobiocin, ceftazidime, ceftriaxone) with inhibition zone diameters >= 20 mm, providing a reference for aquatic drug use. Furthermore, transcriptomic analyses were conducted on the coelomocytes of Apostichopus japonicus infected with P. damselae SCXP. The KEGG enrichment analysis identified distinct signaling pathways that were differentially expressed in the P. damselae SCXP-infected groups at 6, 12, and 24 h post-infection. At the 6-h mark, the focal adhesion pathway exhibited the highest number of differentially expressed genes (DEGs), followed by the PI3K-Akt, mTOR, and MAPK signaling pathways. At 12 h post-infection, the complement and coagulation cascades showed the highest number of DEGs, followed by phagosome-related pathways. By 24 h, the proteasome and lysosome pathways demonstrated an increased number of DEGs. Venn diagram analysis revealed that P. damselae SCXP infection suppressed the expression of several genes, including deoxyribonuclease-1 (DNase I) and acid-sensing ion channel 1-like protein (ASIC1), as validated by qRT-PCR. This study constitutes the first comprehensive investigation into the transcriptomic profile of Apostichopus japonicus coelomocytes in response to P. damselae SCXP infection. Future research endeavors should concentrate on elucidating the roles of DNase I and ASIC1 protein, as these elements may have significant implications for the development of targeted prevention and control strategies. Our study offers a theoretical foundation for the prevention and management of P. damselae-induced SUS in sea cucumbers.
Keyword ：
Photobacterium damselae Photobacterium damselae Sea cucumber Apostichopus japonicus Sea cucumber Apostichopus japonicus Skin ulceration syndrome Skin ulceration syndrome Transcriptomic Transcriptomic
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| GB/T 7714 | Yang, Linwei , Liu, Shuming , Lv, Hui et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Yang, Linwei et al. "Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome" . | AQUACULTURE 612 (2026) . |
| APA | Yang, Linwei , Liu, Shuming , Lv, Hui , Wei, Haiyun , Wu, Tong , Lou, Bilian et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome . | AQUACULTURE , 2026 , 612 . |
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This study investigated the effects of supplementation with recombinant peroxiredoxin IV (Prx IV) on the innate immunity and intestinal health in large yellow croaker (Larimichthys crocea). Fish (n = 160) from triple replicates were fed diets containing 0.00 g kg-1 (Prx1), 0.05 g kg-1 (Prx2), 0.10 g kg-1 (Prx3), and 0.15 g kg-1 (Prx4) Prx IV for 8 weeks. Results demonstrated that Prx IV supplementation significantly improved systemic antioxidant capacity, evidenced by elevated activities of superoxide dismutase and catalase, and increased levels of reduced glutathione. Analysis of the head kidney inflammatory response revealed that dietary Prx IV significantly upregulated gene expression of the anti-inflammatory cytokine interleukin-10 and simultaneously down-regulated the expression of pro-inflammatory cytokines (interleukin 2, interleukin 8, and tumor necrosis factor). Furthermore, western blot analysis confirmed that Prx IV administration markedly suppressed nuclear trans-location of nuclear factor-kappa B p65 in the head kidney. Regarding intestinal effects, dietary Prx IV improved intestinal morphology and significantly enhanced the activities of key digestive enzymes (amylase, lipase, and trypsin). Additionally, Prx IV supplementation significantly upregulated the expression of tight junction proteins (claudin 11 and zona occludens 1, ZO-1). Moreover, dietary administration of 0.15 g kg-1 Prx IV significantly altered intestinal microbiota profiles. Specifically, it increased the abundance of Firmicutes, decreased the abundance of potential pathogens such as Haemophilus parainfluenzae and Klebsiella variicola, and increased the abundance of Bacteroides thetaiotaomicron, a bacterium associated with polysaccharide digestion and absorption. In conclusion, dietary administration of 0.15 g kg-1 Prx IV enhances antioxidant and anti-inflammatory capacities in the head kidney and promotes intestinal health in large yellow croaker. Modulation of the intestinal microbiota may contribute to these beneficial effects.
Keyword ：
Innate immunity Innate immunity Intestinal health Intestinal health Larimichthys crocea Larimichthys crocea Peroxiredoxin IV Peroxiredoxin IV
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| GB/T 7714 | Chen, Zhichu , Liu, Qianqian , Zeng, Chao et al. Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Chen, Zhichu et al. "Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles" . | AQUACULTURE 612 (2026) . |
| APA | Chen, Zhichu , Liu, Qianqian , Zeng, Chao , You, Haokun , Hu, Bing , Zhang, Jiaonan et al. Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles . | AQUACULTURE , 2026 , 612 . |
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Bacterial infection represents a major challenge in large yellow croaker farming, while excessive antibiotic usage has exacerbated the problem of antimicrobial resistance. This situation underscores the critical demand for developing antimicrobial peptides to replace conventional antibiotics. In this study, we designed the antimicrobial peptide RAK18, an 18-amino-acid peptide derived from the C-terminal alpha-helical domain of the large yellow croaker transcription factor Pu.1a, along with its variant RAK18-2, which was engineered through targeted residue substitutions. In vitro assays revealed that RAK18 selectively targeted Pseudomonas plecoglossicida and Bacillus subtilis, whereas RAK18-2 exhibited broader-spectrum antimicrobial activity. Both peptides maintained low cytotoxicity and hemolytic activity at bactericidal concentrations and remained stable under varying temperatures and pH conditions, although salt ion conditions influenced their efficacy. Mechanistic studies showed that they bind to lipopolysaccharide (LPS), inducing bacterial membrane disruption and cell death. In vivo, both RAK18 and RAK18-2 improved survival rates in P. plecoglossicida-infected zebrafish, with RAK18-2 showing superior protective effects. These results not only offer a promising treatment option for managing visceral white nodule disease but also provide the first evidence that the C-terminal alpha-helical peptide of Pu.1 exhibits antibacterial activity, thereby expanding the recognized functional spectrum of Pu.1.
Keyword ：
Antimicrobial peptides Antimicrobial peptides Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) Pu.1 Pu.1 Visceral white nodule disease Visceral white nodule disease
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| GB/T 7714 | He, Hongyu , Zhou, Yuanyuan , Wei, Gonghong et al. Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease [J]. | AQUACULTURE , 2026 , 613 . |
| MLA | He, Hongyu et al. "Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease" . | AQUACULTURE 613 (2026) . |
| APA | He, Hongyu , Zhou, Yuanyuan , Wei, Gonghong , Liao, Yining , Xu, Jing , Chen, Xinhua et al. Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease . | AQUACULTURE , 2026 , 613 . |
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Pseudomonas plecoglossicida is a pathogen bacterium responsible for visceral white spot disease (VWND) in Large Yellow Croakers (Larimichthys crocea), causing significant economic losses in commercial fish farms. The prolinealanine-alanine-arginine repeats protein (PAAR) is a core component of the spike structure in the type VI secretion system (T6SS), which injects toxic effectors into host and contributes to bacterial virulence. However, the role of the PAAR gene in P. plecoglossicida and its impact on bacterial infection and host immune responses remain unexplored. In this study, PAAR-1 was identified for the first time in P. plecoglossicida as an effector gene within the T6SS-1 gene cluster, which is regulated and secreted by T6SS-1. The P. plecoglossicida mutant strain (z.PAAR-1) and its complementary strain (C-z.PAAR-1) were constructed for subsequent investigation. Compared to the wild-type strain, z.PAAR-1 exhibited reduced biofilm formation, adhesion, total antioxidant capacity, and secretion of T6SS core protein Hcp-1. In vitro, z.PAAR-1 showed decreased survival rates in Large Yellow Croaker macrophage cell line (LYC-FM) due to impaired oxidative stress tolerance. In vivo, infection with z.PAAR-1 led to a significant reduction in mortality, bacterial colonization, and the formation of spleen nodules in Large Yellow Croakers. Comparative transcriptome analysis revealed that PAAR-1 predominantly influences the host Toll-like receptor (TLR) signaling pathway and apoptosis by upregulating the expression of plasma membrane-associated TLRs, including TLR1, TLR2, and TLR5, while downregulating the expression of endosomal TLRs like TLR3, TLR7, TLR8, and TLR9, along with its downstream molecules such as MyD88 and TRAF3. Additionally, knockout PAAR1 downregulates apoptosis-related genes including AP-1, NF-kappa B, FAS-L TNF alpha, Caspase8, and FAS-L. Real-time quantitative polymerase chain reaction (RT-qPCR) further confirmed these findings. Furthermore, the proportion of apoptotic cells was significantly lower in the z.PAAR-1 infected LYC-PKM cells. These results indicate that PAAR-1 is involved in regulating TLR signaling pathway and apoptosis in Large Yellow Croaker. This study provides the first identification of the core T6SS-1 gene PAAR-1 in P. plecoglossicida, offering valuable insights into its pathogenic mechanisms and presenting a potential target for attenuated vaccine development.
Keyword ：
Immune response Immune response Large yellow croaker Large yellow croaker PAAR-1 PAAR-1 Pathogenicity Pathogenicity Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Zhang, Baoyu , Li, Youshen , Li, Jianxin et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) [J]. | AQUACULTURE , 2026 , 610 . |
| MLA | Zhang, Baoyu et al. "Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea)" . | AQUACULTURE 610 (2026) . |
| APA | Zhang, Baoyu , Li, Youshen , Li, Jianxin , Zhai, Yu , Meng, Ziyu , Huang, Xiyue et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) . | AQUACULTURE , 2026 , 610 . |
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本发明公开了一种大黄鱼IL‑22重组蛋白，所述重组蛋白为大黄鱼IL‑22成熟肽序列，其氨基酸序列如SEQ ID NO.2所示。本发明通过PCR扩增编码大黄鱼IL‑22成熟肽第31~190位氨基酸的基因片段，转化大肠杆菌进行异源表达，获得重组蛋白。本发明大黄鱼IL‑22重组蛋白可激活大黄鱼头肾细胞系（LYCK）中转录因子STAT3，此外，还可诱导大黄鱼免疫器官中炎症因子（IL‑1β、IL‑6和IL‑8）的表达，表明大黄鱼IL‑22重组蛋白具有明显的免疫调节作用，显示出其在水产免疫调节剂研发中的良好应用前景。
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| GB/T 7714 | 母尹楠 , 陈新华 , 陶帅超 et al. 大黄鱼白细胞介素22重组蛋白及其应用 : CN202510206915.6[P]. | 2025-02-25 . |
| MLA | 母尹楠 et al. "大黄鱼白细胞介素22重组蛋白及其应用" : CN202510206915.6. | 2025-02-25 . |
| APA | 母尹楠 , 陈新华 , 陶帅超 , 任帅卫 . 大黄鱼白细胞介素22重组蛋白及其应用 : CN202510206915.6. | 2025-02-25 . |
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本发明公开了一种大黄鱼白细胞介素4/13B（IL‑4/13B）基因的启动子，所述启动子序列如SEQ ID NO.1所示。将重组质粒pGL3‑IL‑4/13B和对照质粒pGL3‑Basic分别转染鲤上皮瘤EPC细胞，以海肾荧光素酶报告基因质粒pRL‑TK为内参，使用双萤光素酶报告基因检测系统分析不同组间萤火虫荧光素酶活性差异；再分别使用Poly（I:C）、植物凝集素PHA和灭活的变形假单胞菌刺激转染pGL3‑IL‑4/13B质粒的EPC细胞，分析萤火虫荧光素酶活性的变化。本发明提供的大黄鱼 IL‑4/13B 基因的启动子序列，具有启动子基础活性，可启动下游报告基因转录表达，并响应Poly(I:C)、PHA、灭活的变形假单胞菌等外源刺激，为研究大黄鱼 IL‑4/13B 基因的转录调控机制提供优良的实验系统，也可用于高效表达外源基因或应用于转基因大黄鱼的研究，具有理论意义和实践价值。
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| GB/T 7714 | 陈新华 , 母尹楠 , 任帅卫 et al. 大黄鱼白细胞介素4/13B基因启动子序列及其应用 : CN202510206741.3[P]. | 2025-02-25 . |
| MLA | 陈新华 et al. "大黄鱼白细胞介素4/13B基因启动子序列及其应用" : CN202510206741.3. | 2025-02-25 . |
| APA | 陈新华 , 母尹楠 , 任帅卫 , 陶帅超 . 大黄鱼白细胞介素4/13B基因启动子序列及其应用 : CN202510206741.3. | 2025-02-25 . |
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本发明公开了一种大黄鱼SOCS3基因的启动子序列，如SEQ ID NO.1所示。将重组质粒pGL3‑SOCS3转染鲤上皮瘤（EPC）细胞，以海肾荧光素酶为内参，其萤火虫荧光素酶活性最高可达对照组的4.2倍，说明大黄鱼SOCS3启动子启动了下游报告基因的转录表达，具有启动子基础活性。将重组质粒pGL3‑SOCS3和pRL‑TK共转染EPC细胞，再加入poly(I:C)，结果显示，Poly(I:C)可显著增强大黄鱼SOCS3启动子的活性，和对照组相比最高上升了1.7倍，说明大黄鱼SOCS3启动子可以响应外源的免疫刺激。本发明为研究大黄鱼 SOCS3 基因的转录调控机制提供优良的实验系统，也可用于高效表达外源基因或应用于转基因大黄鱼的研究，具有理论意义和实践价值。
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| GB/T 7714 | 母尹楠 , 陈新华 , 陶帅超 et al. 大黄鱼SOCS3基因启动子序列及其应用 : CN202510206474.X[P]. | 2025-02-25 . |
| MLA | 母尹楠 et al. "大黄鱼SOCS3基因启动子序列及其应用" : CN202510206474.X. | 2025-02-25 . |
| APA | 母尹楠 , 陈新华 , 陶帅超 , 任帅卫 . 大黄鱼SOCS3基因启动子序列及其应用 : CN202510206474.X. | 2025-02-25 . |
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本发明属于生物技术领域，具体涉及基于大黄鱼CXCL8蛋白的抗菌肽及其应用。本发明以大黄鱼CXCL8蛋白为模板，截取13个氨基酸的线性多肽，并经羧基端酰胺化修饰，获得抗菌肽AVV13N，抗菌肽AVV13N的氨基酸序列为Ala‑Arg‑Trp‑Val‑Arg‑Arg‑Val‑Leu‑Lys‑Lys‑Lys‑Met‑Val‑NH2；将抗菌肽AVV13N氨基酸序列中第11位的赖氨酸和第13位的缬氨酸进行互换，获得抗菌肽AVK13N，抗菌肽AVK13N的氨基酸序列为Ala‑Arg‑Trp‑Val‑Arg‑Arg‑Val‑Leu‑Lys‑Lys‑Val‑Met‑Lys‑NH2。抗菌肽AVV13N和AVK13N的分子量均为1669.12Da，等电点均为12.44，均为碱性多肽。抗菌肽AVV13N和AVK13N具有广谱杀菌活性，可特异性杀灭革兰氏阳性菌和革兰氏阴性菌。此外，抗菌肽AVV13N和AVK13N均具有较弱的溶血活性、较低的细胞毒性以及良好的温度稳定性、酸碱度稳定性和盐离子稳定性。本发明提供的抗菌肽AVV13N和AVK13N可代替抗生素，应用于细菌性疾病的防治。
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| GB/T 7714 | 陈新华 , 张向阳 , 农燕鸿 et al. 基于大黄鱼CXCL8蛋白的抗菌肽及其应用 : CN202510040575.4[P]. | 2025-01-10 . |
| MLA | 陈新华 et al. "基于大黄鱼CXCL8蛋白的抗菌肽及其应用" : CN202510040575.4. | 2025-01-10 . |
| APA | 陈新华 , 张向阳 , 农燕鸿 , 周园园 . 基于大黄鱼CXCL8蛋白的抗菌肽及其应用 : CN202510040575.4. | 2025-01-10 . |
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本发明提出一种基于YOLOv5算法大黄鱼体表白点病图像采集装置，包括流速模拟水槽（1）；流速模拟水槽包括大黄鱼图像采集区（5）、流速控制区（25）、水环境控制区（13）；大黄鱼图像采集区处设有图像数据采集系统，图像数据采集系统包括多个与电脑相连的摄像头；流速模拟水槽先限制受检大黄鱼的游动动作，使其动作能与图像数据采集系统的工作参数适配，再以图像数据采集系统对大黄鱼体表图像进行采集，基于YOLOv5算法对图像进行分析，或是以采集的图像对YOLOv5算法进行训练；本发明能够采集大量独立的、多角度、清晰的患病大黄鱼的图像数据，也能够模拟养殖环境不同浊度、不同光照强度、群体运动条件下的患病大黄鱼图像数据，使得YOLOv5算法训练效果更加显著。
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| GB/T 7714 | 陈新华 , 龚德华 , 邵建春 et al. 一种基于YOLOv5算法大黄鱼体表白点病图像采集装置 : CN202411473394.2[P]. | 2024-10-22 . |
| MLA | 陈新华 et al. "一种基于YOLOv5算法大黄鱼体表白点病图像采集装置" : CN202411473394.2. | 2024-10-22 . |
| APA | 陈新华 , 龚德华 , 邵建春 , 翟宇 . 一种基于YOLOv5算法大黄鱼体表白点病图像采集装置 : CN202411473394.2. | 2024-10-22 . |
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本发明属于生物技术领域，具体涉及一种敲除deoR基因的变形假单胞菌减毒疫苗。所述变形假单胞菌减毒疫苗包含变形假单胞菌deoR基因敲除株。所述变形假单胞菌deoR基因敲除株为将变形假单胞菌野生型菌株的deoR基因敲除后获得，所述deoR基因的核苷酸序列如SEQ ID NO.1所示。所述变形假单胞菌deoR基因敲除株相对于变形假单胞菌野生株毒力明显减弱，能有效保护实验大黄鱼免受变形假单胞菌的感染，对大黄鱼具有良好的免疫保护效果，进而对大黄鱼内脏白点病起到良好的预防效果，商业开发应用价值高。
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| GB/T 7714 | 陈新华 , 覃盼 , 陈兴甫 et al. 一种敲除deoR基因的变形假单胞菌减毒疫苗 : CN202411535837.6[P]. | 2024-10-31 . |
| MLA | 陈新华 et al. "一种敲除deoR基因的变形假单胞菌减毒疫苗" : CN202411535837.6. | 2024-10-31 . |
| APA | 陈新华 , 覃盼 , 陈兴甫 , 杨景松 , 吴彤 . 一种敲除deoR基因的变形假单胞菌减毒疫苗 : CN202411535837.6. | 2024-10-31 . |
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