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学者姓名：陈新华
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Abstract ：
Sea cucumber skin ulceration syndrome (SUS) is associated with a diverse range of bacterial and parasitic pathogens. Nonetheless, research efforts aimed at identifying novel pathogens and developing effective prevention and control strategies remain limited. In this study, we identified a strain of Photobacterium damselae and designated it as P. damselae sea cucumber xiapu (P. damselae SCXP), recognizing it as a new pathogenic bacterium associated with SUS in Apostichopus japonicus. We developed a detection method using a TaqMan probebased qPCR assay specific to P. damselae SCXP, which supports the rapid diagnosis of this pathogen. Additionally, artificial infection experiments confirmed that P. damselae SCXP facilitates the progression of SUS in Apostichopus japonicus. Drug sensitivity analysis revealed that the strain was highly sensitive to 16 antibiotics (e. g., novobiocin, ceftazidime, ceftriaxone) with inhibition zone diameters >= 20 mm, providing a reference for aquatic drug use. Furthermore, transcriptomic analyses were conducted on the coelomocytes of Apostichopus japonicus infected with P. damselae SCXP. The KEGG enrichment analysis identified distinct signaling pathways that were differentially expressed in the P. damselae SCXP-infected groups at 6, 12, and 24 h post-infection. At the 6-h mark, the focal adhesion pathway exhibited the highest number of differentially expressed genes (DEGs), followed by the PI3K-Akt, mTOR, and MAPK signaling pathways. At 12 h post-infection, the complement and coagulation cascades showed the highest number of DEGs, followed by phagosome-related pathways. By 24 h, the proteasome and lysosome pathways demonstrated an increased number of DEGs. Venn diagram analysis revealed that P. damselae SCXP infection suppressed the expression of several genes, including deoxyribonuclease-1 (DNase I) and acid-sensing ion channel 1-like protein (ASIC1), as validated by qRT-PCR. This study constitutes the first comprehensive investigation into the transcriptomic profile of Apostichopus japonicus coelomocytes in response to P. damselae SCXP infection. Future research endeavors should concentrate on elucidating the roles of DNase I and ASIC1 protein, as these elements may have significant implications for the development of targeted prevention and control strategies. Our study offers a theoretical foundation for the prevention and management of P. damselae-induced SUS in sea cucumbers.
Keyword ：
Photobacterium damselae Photobacterium damselae Sea cucumber Apostichopus japonicus Sea cucumber Apostichopus japonicus Skin ulceration syndrome Skin ulceration syndrome Transcriptomic Transcriptomic
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| GB/T 7714 | Yang, Linwei , Liu, Shuming , Lv, Hui et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Yang, Linwei et al. "Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome" . | AQUACULTURE 612 (2026) . |
| APA | Yang, Linwei , Liu, Shuming , Lv, Hui , Wei, Haiyun , Wu, Tong , Lou, Bilian et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome . | AQUACULTURE , 2026 , 612 . |
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Pseudomonas plecoglossicida is a pathogen bacterium responsible for visceral white spot disease (VWND) in Large Yellow Croakers (Larimichthys crocea), causing significant economic losses in commercial fish farms. The prolinealanine-alanine-arginine repeats protein (PAAR) is a core component of the spike structure in the type VI secretion system (T6SS), which injects toxic effectors into host and contributes to bacterial virulence. However, the role of the PAAR gene in P. plecoglossicida and its impact on bacterial infection and host immune responses remain unexplored. In this study, PAAR-1 was identified for the first time in P. plecoglossicida as an effector gene within the T6SS-1 gene cluster, which is regulated and secreted by T6SS-1. The P. plecoglossicida mutant strain (z.PAAR-1) and its complementary strain (C-z.PAAR-1) were constructed for subsequent investigation. Compared to the wild-type strain, z.PAAR-1 exhibited reduced biofilm formation, adhesion, total antioxidant capacity, and secretion of T6SS core protein Hcp-1. In vitro, z.PAAR-1 showed decreased survival rates in Large Yellow Croaker macrophage cell line (LYC-FM) due to impaired oxidative stress tolerance. In vivo, infection with z.PAAR-1 led to a significant reduction in mortality, bacterial colonization, and the formation of spleen nodules in Large Yellow Croakers. Comparative transcriptome analysis revealed that PAAR-1 predominantly influences the host Toll-like receptor (TLR) signaling pathway and apoptosis by upregulating the expression of plasma membrane-associated TLRs, including TLR1, TLR2, and TLR5, while downregulating the expression of endosomal TLRs like TLR3, TLR7, TLR8, and TLR9, along with its downstream molecules such as MyD88 and TRAF3. Additionally, knockout PAAR1 downregulates apoptosis-related genes including AP-1, NF-kappa B, FAS-L TNF alpha, Caspase8, and FAS-L. Real-time quantitative polymerase chain reaction (RT-qPCR) further confirmed these findings. Furthermore, the proportion of apoptotic cells was significantly lower in the z.PAAR-1 infected LYC-PKM cells. These results indicate that PAAR-1 is involved in regulating TLR signaling pathway and apoptosis in Large Yellow Croaker. This study provides the first identification of the core T6SS-1 gene PAAR-1 in P. plecoglossicida, offering valuable insights into its pathogenic mechanisms and presenting a potential target for attenuated vaccine development.
Keyword ：
Immune response Immune response Large yellow croaker Large yellow croaker PAAR-1 PAAR-1 Pathogenicity Pathogenicity Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Zhang, Baoyu , Li, Youshen , Li, Jianxin et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) [J]. | AQUACULTURE , 2026 , 610 . |
| MLA | Zhang, Baoyu et al. "Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea)" . | AQUACULTURE 610 (2026) . |
| APA | Zhang, Baoyu , Li, Youshen , Li, Jianxin , Zhai, Yu , Meng, Ziyu , Huang, Xiyue et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) . | AQUACULTURE , 2026 , 610 . |
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This study investigated the effects of supplementation with recombinant peroxiredoxin IV (Prx IV) on the innate immunity and intestinal health in large yellow croaker (Larimichthys crocea). Fish (n = 160) from triple replicates were fed diets containing 0.00 g kg-1 (Prx1), 0.05 g kg-1 (Prx2), 0.10 g kg-1 (Prx3), and 0.15 g kg-1 (Prx4) Prx IV for 8 weeks. Results demonstrated that Prx IV supplementation significantly improved systemic antioxidant capacity, evidenced by elevated activities of superoxide dismutase and catalase, and increased levels of reduced glutathione. Analysis of the head kidney inflammatory response revealed that dietary Prx IV significantly upregulated gene expression of the anti-inflammatory cytokine interleukin-10 and simultaneously down-regulated the expression of pro-inflammatory cytokines (interleukin 2, interleukin 8, and tumor necrosis factor). Furthermore, western blot analysis confirmed that Prx IV administration markedly suppressed nuclear trans-location of nuclear factor-kappa B p65 in the head kidney. Regarding intestinal effects, dietary Prx IV improved intestinal morphology and significantly enhanced the activities of key digestive enzymes (amylase, lipase, and trypsin). Additionally, Prx IV supplementation significantly upregulated the expression of tight junction proteins (claudin 11 and zona occludens 1, ZO-1). Moreover, dietary administration of 0.15 g kg-1 Prx IV significantly altered intestinal microbiota profiles. Specifically, it increased the abundance of Firmicutes, decreased the abundance of potential pathogens such as Haemophilus parainfluenzae and Klebsiella variicola, and increased the abundance of Bacteroides thetaiotaomicron, a bacterium associated with polysaccharide digestion and absorption. In conclusion, dietary administration of 0.15 g kg-1 Prx IV enhances antioxidant and anti-inflammatory capacities in the head kidney and promotes intestinal health in large yellow croaker. Modulation of the intestinal microbiota may contribute to these beneficial effects.
Keyword ：
Innate immunity Innate immunity Intestinal health Intestinal health Larimichthys crocea Larimichthys crocea Peroxiredoxin IV Peroxiredoxin IV
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| GB/T 7714 | Chen, Zhichu , Liu, Qianqian , Zeng, Chao et al. Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Chen, Zhichu et al. "Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles" . | AQUACULTURE 612 (2026) . |
| APA | Chen, Zhichu , Liu, Qianqian , Zeng, Chao , You, Haokun , Hu, Bing , Zhang, Jiaonan et al. Dietary administration of recombinant peroxiredoxin IV contributes to innate immunity and intestinal health in Larimichthys crocea juveniles . | AQUACULTURE , 2026 , 612 . |
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Bacterial infection represents a major challenge in large yellow croaker farming, while excessive antibiotic usage has exacerbated the problem of antimicrobial resistance. This situation underscores the critical demand for developing antimicrobial peptides to replace conventional antibiotics. In this study, we designed the antimicrobial peptide RAK18, an 18-amino-acid peptide derived from the C-terminal alpha-helical domain of the large yellow croaker transcription factor Pu.1a, along with its variant RAK18-2, which was engineered through targeted residue substitutions. In vitro assays revealed that RAK18 selectively targeted Pseudomonas plecoglossicida and Bacillus subtilis, whereas RAK18-2 exhibited broader-spectrum antimicrobial activity. Both peptides maintained low cytotoxicity and hemolytic activity at bactericidal concentrations and remained stable under varying temperatures and pH conditions, although salt ion conditions influenced their efficacy. Mechanistic studies showed that they bind to lipopolysaccharide (LPS), inducing bacterial membrane disruption and cell death. In vivo, both RAK18 and RAK18-2 improved survival rates in P. plecoglossicida-infected zebrafish, with RAK18-2 showing superior protective effects. These results not only offer a promising treatment option for managing visceral white nodule disease but also provide the first evidence that the C-terminal alpha-helical peptide of Pu.1 exhibits antibacterial activity, thereby expanding the recognized functional spectrum of Pu.1.
Keyword ：
Antimicrobial peptides Antimicrobial peptides Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) Pu.1 Pu.1 Visceral white nodule disease Visceral white nodule disease
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| GB/T 7714 | He, Hongyu , Zhou, Yuanyuan , Wei, Gonghong et al. Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease [J]. | AQUACULTURE , 2026 , 613 . |
| MLA | He, Hongyu et al. "Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease" . | AQUACULTURE 613 (2026) . |
| APA | He, Hongyu , Zhou, Yuanyuan , Wei, Gonghong , Liao, Yining , Xu, Jing , Chen, Xinhua et al. Pu.1a-derived antimicrobial peptides from large yellow croaker (Larimichthys crocea) with a potential application in treatment of visceral white nodule disease . | AQUACULTURE , 2026 , 613 . |
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Eucommia ulmoides leaf extract (ELE) and Astragalus polysaccharides (APS) have been widely used as immunopotentiators in aquaculture. Our prior research on large yellow croaker (Larimichthys crocea) demonstrated that dietary 1 g/kg APS bolstered fish immunity and antioxidant defense. However, the combined effect of ELE and APS in juvenile large yellow croaker remains unknown. Hence, this study aimed to investigate the synergistic effect of ELE and APS on the growth, antioxidant capacity, and intestinal inflammation in large yellow croaker. A total of 1200 fish were divided into five groups and fed diets with 1 g/kg APS and varying ELE levels: 0 g/kg (ELE0), 0.25 g/kg (ELE0.25), 0.5 g/kg (ELE0.5), 1 g/kg (ELE1), and 2 g/kg (ELE2). After an 8-week feeding period, the ELE0.5 and ELE1 groups showed superior weight gain rate, specific growth rate, and feed efficiency compared to other groups. The ELE1 group also had elevated trypsin and lipase activities in the intestine, whereas alpha-amylase activity was not influenced by ELE addition. Antioxidant enzyme activities, such as hepatopancreas superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the ELE1 group were significantly enhanced, while malondialdehyde (MDA) levels decreased with increasing ELE. Intestinal morphology revealed the highest villi height in proximal and distal intestines of ELE1 group, with no significant change in mucosal thickness. In terms of cytokines, the ELE1 group showed significant down-regulation of pro-inflammatory (tnf-alpha, il-1 beta and il-6) and up-regulation of anti-inflammatory (il-4/13a, il-10 and tgf-beta) markers, modulated by MAPK and mTOR signaling. In conclusion, this study indicates that supplementing diets with 1 g/kg ELE alongside 1 g/kg APS in juvenile large yellow croaker offers the best synergistic effect on fish immunity, including enhanced growth, antioxidant capacity, and relieved intestinal inflammation through MAPK and mTOR signaling.
Keyword ：
Anti-inflammation Anti-inflammation Antioxidant capacity Antioxidant capacity Astragalus polysaccharides Astragalus polysaccharides Eucommia ulmoides leaf extracts Eucommia ulmoides leaf extracts Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea )
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| GB/T 7714 | Shao, Jianchun , Wang, Xuexi , Liu, Qianqian et al. Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
| MLA | Shao, Jianchun et al. "Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 161 (2025) . |
| APA | Shao, Jianchun , Wang, Xuexi , Liu, Qianqian , Lv, Huiyuan , Qi, Qiong , Li, Changhui et al. Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
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Dietary Astragalus polysaccharides (APS) get wide application in aquaculture due to their excellent immunoregulatory effects. However, little is known about the effects of dietary APS on vaccine potency in fish. In the present study, large yellow croakers (Larimichthys crocea) were injected with formalin-inactivated Pseudomonas plecoglossicida after APS feeding for 14 d and then challenged by live P. plecoglossicida on 28 d post-vaccination. The results showed that dietary APS combined with inactivated vaccine could improve the survival rate, and alleviate splenic lesions and bacteria load post-challenge, thus exhibiting a better protection in large yellow croaker against P. plecoglossicida infection than inactivated vaccine treatment alone. Fish in APS + P. plecoglossicida vaccine group expressed a better antioxidant status by possessing a relatively higher serum total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity and a significantly lower malondialdehyde (MDA) content than those in vaccine alone group. Serum lysozyme (LZM) and alkaline phosphatase (AKP) activities, and immunoglobulin M (IgM) titers were all improved in fish of APS + P. plecoglossicida vaccine group compared to fish in vaccine group. Furthermore, fish in APS + P. plecoglossicida vaccine group showed a lower down-regulation of pro-inflammatory cytokines interleukin-1 beta (IL-1 beta) and IL-6, and a higher up-regulation of antiinflammatory cytokine IL-10, immunoglobulin (IgM) and T cell immunity-related cytokines, interferon-gamma (IFN-gamma), IL-4/13A, and IL-4/13B, when compared with those in fish of vaccine group. These results suggested that dietary APS could assist inactivated vaccine to trigger stronger innate and adaptive immune responses against P. plecoglossicida infection. These findings further uncover the immunoregulatory mechanism of dietary APS, and provide valuable information for prevention and control of bacteriosis in fish.
Keyword ：
Astragalus polysaccharides Astragalus polysaccharides Immune response Immune response Inactivated vaccine Inactivated vaccine Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea ) Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Song, Yueyang , Chen, Hui , An, Huimin et al. Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker ( Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 157 . |
| MLA | Song, Yueyang et al. "Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker ( Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 157 (2025) . |
| APA | Song, Yueyang , Chen, Hui , An, Huimin , Wang, Yongyang , Shao, Jianchun , Yan, Meijiao et al. Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker ( Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 157 . |
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Spleen tyrosine kinase (SYK), a non-receptor protein tyrosine kinase, is a key component of B cell receptor signaling and can regulate multiple physiological functions of B cells in mammals. In this study, a SYK gene was cloned and characterized from large yellow croaker (Larimichthys crocea) (LcSYK), whose open reading frame consists of 1851 base pairs and encodes 616 amino acid residues. The predicted LcSYK protein contains two Nterminal tandem Src homology 2 domains and a C-terminal tyrosine kinase catalytic domain, and shares a high amino acid sequence identity with SYK sequences in other vertebrate species. LcSYK was mainly expressed in immune tissues, such as head kidney, trunk kidney, spleen, and gill. The mRNA expression of LcSYK in primary head kidney leukocytes was not changed at 12, 24, and 48 h after lipopolysaccharide (LPS) stimulation. LPS stimulation upregulated the mRNA expression and protein production of IgM in IgM+ B cells, accompanied by an increase in the phosphorylation level, but not the total protein level, of LcSYK. Moreover, when we used PRT062607 HCl to inhibit the phosphorylation of LcSYK, both mRNA expression and protein production of IgM in IgM+ B cells were significantly suppressed. These results suggest that SYK phosphorylation may play a role in LPS-induced IgM production by IgM+ B cells, improving our understanding of the role of SYK in immunoglobulin responses of B cells in fish.
Keyword ：
B cell B cell Immunoglobulin Immunoglobulin Large yellow croaker Large yellow croaker SYK SYK
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| GB/T 7714 | Xie, Hongjun , Cao, Shuangshuang , Chen, Yueming et al. The role of SYK phosphorylation in LPS-induced immunoglobulin responses of B cells in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
| MLA | Xie, Hongjun et al. "The role of SYK phosphorylation in LPS-induced immunoglobulin responses of B cells in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 161 (2025) . |
| APA | Xie, Hongjun , Cao, Shuangshuang , Chen, Yueming , Wang, Zhiqiang , Chen, Xinhua , Cui, Zhengwei . The role of SYK phosphorylation in LPS-induced immunoglobulin responses of B cells in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
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This study aimed to investigate the immune mechanisms of pedal mucus in Pacific abalone Haliotis discus hannai from different populations. Proteomic differences between the pedal mucus of the control group and the heat-resistance group of H. discus hannai were comparatively analyzed, and the proteins were annotated and analyzed to understand the related functions and roles of differentially expressed proteins (DEPs). The results showed that there were 4054 DEPs in total, among which the DEPs in the pedal mucus of the heat-resistance group and the control group were mainly immune-related heat shock proteins, calmodulin, Ig-like and fibronectin type III domain-containing proteins, histones and mucins, etc.; the DEPs related to growth metabolism included glutathione, growth factor receptor-bound protein, alanine aminotransferase, etc. Circadian entrainment signaling pathway and growth hormone synthesis, secretion and action signaling pathway was significantly enriched, cortisol synthesis and secretion signaling pathway was up-regulated in Haliotis discus hannai heat-resistance group. This study provided references for exploring the relationship between the mucus proteome difference in pedal mucus from different abalone populations sources. These results would contribute to further search for proteins related to immunity, growth and stress resistance, and provide theoretical basis for the development of high-quality germplasm resources of Pacific abalone.
Keyword ：
Enrichment analysis Enrichment analysis Haliotis discus hannai Haliotis discus hannai Pedal mucus Pedal mucus Proteomics Proteomics
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| GB/T 7714 | Di, Guilan , Zhang, Yu , Jiang, Mingmei et al. 4D-FastDIA proteomic analysis of pedal mucus in Pacific abalone Haliotis discus hannai heat-resistance group [J]. | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS , 2025 , 55 . |
| MLA | Di, Guilan et al. "4D-FastDIA proteomic analysis of pedal mucus in Pacific abalone Haliotis discus hannai heat-resistance group" . | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 55 (2025) . |
| APA | Di, Guilan , Zhang, Yu , Jiang, Mingmei , Zhang, Weini , Wu, Yunlong , Ma, Zeyuan et al. 4D-FastDIA proteomic analysis of pedal mucus in Pacific abalone Haliotis discus hannai heat-resistance group . | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS , 2025 , 55 . |
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Melanoma differentiation-associated gene 5 (MDA5) initiates type I interferon (IFN) production by detecting cytosolic viral RNA. Mammalian MDA5 is an IFN-inducible gene and controlled by IFN regulatory factor 1 (IRF1). Teleost MDA5 also induces type I IFN production in response to viruses, yet its regulation remains largely unexplored. This study used the large yellow croaker Larimichthys crocea (Lc) as a model organism and revealed that a type I IFN (LcIFNi) triggers the expression of LcMDA5 through the JAK-STAT signaling pathway, which involves phosphorylation of LcIRF11. LcMDA5 was transcriptionally regulated by LcIRF11. Mechanistically, LcIRF11 interacts with the IFN-stimulated response element within the LcMDA5 promoter, via a3 helix and loop1, and loop2 and loop3 in its DNA binding domain. Overexpression of LcIRF11 recruits p300 and RNA polymerase II (Pol II) to the LcMDA5 promoter region. Pull-down analysis further confirmed the interaction of LcIRF11 with these two proteins. This recruitment was accompanied by increased levels of his- tone H3K27 acetylation (H3K27ac) and histone H3K4 trimethylation (H3K4me3), both of which are strongly associated with active transcription. Conversely, silencing LcIRF11 reduced p300 and Pol II recruitments and hindered the enrichment of H3K27ac/H3K4me3 modifications at the LcMDA5 promoter. Thus, here we present the first report of IRF11 orchestrating the activation of MDA5 transcription by binding to the IFN-stimulated response element of MDA5 promoter and forming a transcriptional complex with p300 and Pol II. Our results revealed an ancient regulatory mechanism of MDA5 in lower vertebrates, providing insights into its function and evolution.
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| GB/T 7714 | Li, Wenxing , Feng, Yuan , Teng, Yan et al. P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11 [J]. | JOURNAL OF BIOLOGICAL CHEMISTRY , 2025 , 301 (2) . |
| MLA | Li, Wenxing et al. "P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11" . | JOURNAL OF BIOLOGICAL CHEMISTRY 301 . 2 (2025) . |
| APA | Li, Wenxing , Feng, Yuan , Teng, Yan , Montero, Alvaro Fernandez , Zhou, Yuanyuan , Zhang, Xiangyang et al. P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11 . | JOURNAL OF BIOLOGICAL CHEMISTRY , 2025 , 301 (2) . |
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Ocean acidification (OA) and seawater salinity are two major environmental factors that influence the growth and distribution of macroalgae in coastal ecosystems. To investigate the effects of OA and salinity on the invasive macroalga Codium fragile, the growth, Chlorophyll a fluorescence, and biochemical compositions (pigment and soluble carbohydrate contents, the superoxide dismutase (SOD) activity, and malondialdehyde (MDA) contents) were studied after exposure to two pCO2 levels (400 ppmv, LC; and 1000 ppmv, HC) and four salinity regimes (high salinity, 40 psu; control salinity, 30 psu; medium salinity, 20 psu; low salinity, 10 psu). The results showed that, except for SOD activity at 20 psu, the growth, maximum and effective quantum yield of PSII, and maximum relative electron transport, pigment and soluble carbohydrate contents, SOD activity, and the MDA content were adversely impacted by both hypo- and hypersaline under LC conditions. Similarly, under HC conditions, the growth, photosynthetic physiology and biochemistry were negatively impacted by low salinity, while high salinity enhanced pigment contents and chlorophyll fluorescence parameters but inhibited SOD activity and MDA contents. Furthermore, higher pCO2 significantly promoted growth, pigment contents, and photosynthetic performance at 20 and 40 psu, while it amplified the depression in growth at 10 psu. These findings suggest that OA may enhance the potential invasive ability and salinity tolerance of C. fragile under medium hyposaline and hypersaline conditions by alleviating the negative effects of salinity stress on growth, photosynthesis, and pigments synthesis. However, it may also synergistically reduce algal growth at further reduced salinity. These data collected herein are valuable for understanding C. fragile cultivation and predicting its future distribution in response to changing ocean conditions.
Keyword ：
Codium fragile Codium fragile Growth Growth Invasive macroalgae Invasive macroalgae Ocean acidification Ocean acidification Photosynthetic physiology Photosynthetic physiology Salinity Salinity
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| GB/T 7714 | Chu, Yaoyao , Shi, Yunyun , Xue, Yuning et al. Salinity-dependent effects of seawater acidification on growth, photosynthetic physiology and biochemistry of the invasive macroalga Codium fragile [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 88 . |
| MLA | Chu, Yaoyao et al. "Salinity-dependent effects of seawater acidification on growth, photosynthetic physiology and biochemistry of the invasive macroalga Codium fragile" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 88 (2025) . |
| APA | Chu, Yaoyao , Shi, Yunyun , Xue, Yuning , He, Lianghua , Li, Xiaodong , Shi, Xiangzhu et al. Salinity-dependent effects of seawater acidification on growth, photosynthetic physiology and biochemistry of the invasive macroalga Codium fragile . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 88 . |
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