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学者姓名：王传奇
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Abstract ：
The DNA damage-regulated autophagy modulators have been identified as key activators of autophagy, an emerging mechanism to combat viral infections. In this study, we cloned and characterized the DNA damageregulated autophagy modulator 2 homolog from the shrimp Penaeus vannamei (designated as PvDRAM2) and investigated its potential roles during white spot syndrome virus (WSSV) infection. The predicted threedimensional structure of PvDRAM2 consists of six beta-fold regions, which exhibit conservation with similar structures in other species. Phylogenetic analysis revealed that the clusters of PvDRAM2 evolved alongside invertebrate DRAMs, showing a close relationship with DRAMs from Penaeus japonicus and Homarus americanus. Quantitative real-time PCR (qRT-PCR) analysis indicated that PvDRAM2 is ubiquitously expressed across various shrimp tissues, with significantly increased expression levels in hemocytes and intestines following WSSV challenge. RNA interference (RNAi)-mediated knockdown of PvDRAM2 notably elevated WSSV loads and reduced survival rates in shrimps subjected to WSSV challenge. Functionally, the overexpression of PvDRAM2 enhanced autophagy in High Five cells. Additionally, the application of autophagy activators demonstrated that activating autophagy could counteract the increased WSSV replication precipitated by the knockdown of PvDRAM2. Collectively, our findings suggest that PvDRAM2 may inhibit WSSV infection by activating autophagy.
Keyword ：
Autophagy Autophagy DRAM2 DRAM2 Penaeus vannamei Penaeus vannamei WSSV WSSV
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| GB/T 7714 | Huang, Xiyu , Zhao, Mingming , Huang, Jieyu et al. DRAM2 inhibits white spot syndrome virus infection via activating autophagy in Penaeus vannamei [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
| MLA | Huang, Xiyu et al. "DRAM2 inhibits white spot syndrome virus infection via activating autophagy in Penaeus vannamei" . | FISH & SHELLFISH IMMUNOLOGY 160 (2025) . |
| APA | Huang, Xiyu , Zhao, Mingming , Huang, Jieyu , Liu, Mingjian , Yang, Linwei , Wang, Chuanqi . DRAM2 inhibits white spot syndrome virus infection via activating autophagy in Penaeus vannamei . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
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Abstract ：
C-type lectins (CTLs), a vital group of soluble pattern recognition receptors (PRRs), are essential to the shrimp's innate immune system. However, little is known about the CTLs composed of a C-type lectin domain (CTLD) and another domain. In this study, a novel C-type lectin containing a leucine-rich repeat (LRR) domain, designated LRRCTL, was identified in Penaeus vannamei and found to exhibit immune regulatory functions. First, LRRCTL expression was highest in the hepatopancreas. The mRNA expression levels of LRRCTL showed a significant upregulation under the different stimuli, including Vibrio parahaemolyticus, Staphylococcus aureus, Aspergillus niger, Lipopolysaccharide (LPS). Second, knockdown of the expression of LRRCTL increased the bacterial load of V. parahaemolyticus, whereas injection of rLRRCTL or r triangle LRR-LRRCTL recombinant proteins enhanced the survival rate of V. parahaemolyticus-infected shrimp. Additionally, the knockdown of LRRCTL expression reduced the mRNA level of multiple immune-related genes, encompassing both immune signaling components and antimicrobial peptide genes, suggesting an important role in immune regulation. Our findings reveal that LRRCTL is essential for regulating humoral immune responses, particularly antimicrobial peptide production, against V. parahaemolyticus infection. The findings provide a theoretical foundation for further investigation into the functional mechanisms of composite CTLs in shrimp innate immunity and potential molecular targets for developing strategies to prevent and control shrimp diseases.
Keyword ：
Immune regulatory Immune regulatory LRRCTL LRRCTL Penaeus vannamei Penaeus vannamei Vibrio parahaemolyticus Vibrio parahaemolyticus
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| GB/T 7714 | Weng, Kejia , Liu, Ruoyu , Xiong, Chengqi et al. A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 165 . |
| MLA | Weng, Kejia et al. "A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection" . | FISH & SHELLFISH IMMUNOLOGY 165 (2025) . |
| APA | Weng, Kejia , Liu, Ruoyu , Xiong, Chengqi , Shi, Yuxin , Chen, Wenqian , Wang, Chuanqi et al. A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 165 . |
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