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学者姓名：崔正伟
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Abstract ：
Sea cucumber skin ulceration syndrome (SUS) is associated with a diverse range of bacterial and parasitic pathogens. Nonetheless, research efforts aimed at identifying novel pathogens and developing effective prevention and control strategies remain limited. In this study, we identified a strain of Photobacterium damselae and designated it as P. damselae sea cucumber xiapu (P. damselae SCXP), recognizing it as a new pathogenic bacterium associated with SUS in Apostichopus japonicus. We developed a detection method using a TaqMan probebased qPCR assay specific to P. damselae SCXP, which supports the rapid diagnosis of this pathogen. Additionally, artificial infection experiments confirmed that P. damselae SCXP facilitates the progression of SUS in Apostichopus japonicus. Drug sensitivity analysis revealed that the strain was highly sensitive to 16 antibiotics (e. g., novobiocin, ceftazidime, ceftriaxone) with inhibition zone diameters >= 20 mm, providing a reference for aquatic drug use. Furthermore, transcriptomic analyses were conducted on the coelomocytes of Apostichopus japonicus infected with P. damselae SCXP. The KEGG enrichment analysis identified distinct signaling pathways that were differentially expressed in the P. damselae SCXP-infected groups at 6, 12, and 24 h post-infection. At the 6-h mark, the focal adhesion pathway exhibited the highest number of differentially expressed genes (DEGs), followed by the PI3K-Akt, mTOR, and MAPK signaling pathways. At 12 h post-infection, the complement and coagulation cascades showed the highest number of DEGs, followed by phagosome-related pathways. By 24 h, the proteasome and lysosome pathways demonstrated an increased number of DEGs. Venn diagram analysis revealed that P. damselae SCXP infection suppressed the expression of several genes, including deoxyribonuclease-1 (DNase I) and acid-sensing ion channel 1-like protein (ASIC1), as validated by qRT-PCR. This study constitutes the first comprehensive investigation into the transcriptomic profile of Apostichopus japonicus coelomocytes in response to P. damselae SCXP infection. Future research endeavors should concentrate on elucidating the roles of DNase I and ASIC1 protein, as these elements may have significant implications for the development of targeted prevention and control strategies. Our study offers a theoretical foundation for the prevention and management of P. damselae-induced SUS in sea cucumbers.
Keyword ：
Photobacterium damselae Photobacterium damselae Sea cucumber Apostichopus japonicus Sea cucumber Apostichopus japonicus Skin ulceration syndrome Skin ulceration syndrome Transcriptomic Transcriptomic
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| GB/T 7714 | Yang, Linwei , Liu, Shuming , Lv, Hui et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Yang, Linwei et al. "Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome" . | AQUACULTURE 612 (2026) . |
| APA | Yang, Linwei , Liu, Shuming , Lv, Hui , Wei, Haiyun , Wu, Tong , Lou, Bilian et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome . | AQUACULTURE , 2026 , 612 . |
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The coagulation system is a mechanism for wound healing after injury, but it also participates in host early immune defense. The coagulation factor VII (FVII) can initiate extrinsic pathway and play an important role in the coagulation process. However, studies of the immune function of FVII are scarce, especially in fish. In this study, we cloned and characterized an FVII-like gene from large yellow croaker (Larimichthys crocea) (LcFVIIL). The open reading frame of LcFVIIL consists of 1437 base pairs and encodes 478 amino acid residues. LcFVIIL contains conserved domains that are present in other vertebrate FVIIs or FVIILs, including a prepropeptide, a gamma-carboxy glutamic acid domain, two epidermal growth factor-like domains, and a serine protease domain. LcFVIIL was highly expressed in the liver and brain, but its expression was low in the other tested tissues. At the cellular level, LcFVIIL was highly expressed in macrophages, and its expression was induced by exposure to Pseudomonas plecoglossicida. We produced the recombinant LcFVIIL light chain (rLcFVIIL-LC), and found that it had obvious antibacterial effects against Gram-positive bacteria but low against Gram-negative bacteria. The rLcFVIIL-LC promoted the proliferation of macrophages. It also significantly induced the expression of proinflammatory factor (IL-1(3 and IL-6) and increased reactive oxygen species activity in large yellow croaker macrophages, while inhibited the expression of anti-inflammatory factor (TGF-(3), suggesting that rLcFVIIL-LC may promote polarization of macrophages towards the M1 type. Taken together, these findings provide insight into the function of fish FVII, and advance our understanding of the role of the coagulation system in host defense.
Keyword ：
Coagulation factor VII Coagulation factor VII Large yellow croaker Large yellow croaker Macrophage Macrophage Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Chen, Yueming , Zhao, Han , Cao, Shuangshuang et al. Molecular characterization of large yellow croaker (Larimichthys crocea) coagulation factor VII-like and its function on macrophage proliferation and polarization [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 158 . |
| MLA | Chen, Yueming et al. "Molecular characterization of large yellow croaker (Larimichthys crocea) coagulation factor VII-like and its function on macrophage proliferation and polarization" . | FISH & SHELLFISH IMMUNOLOGY 158 (2025) . |
| APA | Chen, Yueming , Zhao, Han , Cao, Shuangshuang , Xie, Hongjun , Huang, Jieyu , Chen, Xinhua et al. Molecular characterization of large yellow croaker (Larimichthys crocea) coagulation factor VII-like and its function on macrophage proliferation and polarization . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 158 . |
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The large yellow croaker (Larimichthys crocea), an economically important marine fish in China, often suffers serious losses due to visceral white nodule disease caused by Pseudomonas plecoglossicida infection. IgM+ B cells play critical roles in the defense against bacterial infection, yet their immune response and underlying mechanisms against the P. plecoglossicida infection in large yellow croaker remain poorly characterized. In this study, we used fluorescence-activated cell sorting (FACS) and RNA sequencing to profile the transcriptomes of head kidney IgM+ B cells at different time points after P. plecoglossicida infection. The results showed that P. plecoglossicida infection induced time-dependent transcriptomic changes in IgM+ B cells. Mfuzz analysis categorized genes into 9 different clusters based on their expression patterns. Notably, cluster 9, 8 and 1 exhibited specific upregulation at 1, 3, and 7 days post infection (dpi), respectively, while cluster 2 displayed a sustained upregulation pattern throughout infection. KEGG enrichment analysis revealed enhanced energy metabolism and proliferation ability of IgM+ B cells after infection. Furthermore, we found that the MAPK signaling pathway was rapidly activated after infection (enriched in cluster 9). qPCR results further confirmed the upregulation of MAPK signaling related genes (cacna1g, map2k1, map3k4, map3k10) at 1 dpi, suggesting its role in regulating the early immune response of B cells. Together, this study comprehensively analyzed the transcriptomic dynamics of IgM+ B cells after P. plecoglossicida infection, which would provide insights into the understanding of teleost B cell immunity and offer potential strategies for bacterial disease control in large yellow croaker aquaculture.
Keyword ：
IgM plus B cells IgM plus B cells Large yellow croaker Large yellow croaker MAPK signaling MAPK signaling Pseudomonas plecoglossicida Pseudomonas plecoglossicida RNA-seq RNA-seq
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| GB/T 7714 | Zhu, Zhuo , Chen, Qiuxuan , Li, Qiuhua et al. Transcriptomic analysis reveals the role of MAPK signaling pathway in IgM plus B cells against Pseudomonas plecoglossicida infection in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 165 . |
| MLA | Zhu, Zhuo et al. "Transcriptomic analysis reveals the role of MAPK signaling pathway in IgM plus B cells against Pseudomonas plecoglossicida infection in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 165 (2025) . |
| APA | Zhu, Zhuo , Chen, Qiuxuan , Li, Qiuhua , Wang, Meiyan , Zhang, Yihan , Cui, Zhengwei et al. Transcriptomic analysis reveals the role of MAPK signaling pathway in IgM plus B cells against Pseudomonas plecoglossicida infection in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 165 . |
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Immunoglobulin M (IgM) is considered the most ancient and prevalent Ig in fish, which plays important roles in systemic and mucosal immunity. In the current study, two heavy chain genes of IgM (named Igh mu 1 and Igh mu 2) were cloned in Nile tilapia (Oreochromis niloticus), and the amino acid identity between the constant regions of the two IgM heavy chains were 82.58 %. Cysteine and tryptophan residues that are crucial for forming disulfide bonds and the folding of the Ig domains were conserved. Genomic localization showed that the two Igh mu s were adjacent within the same IgM locus. By mass spectrum detection in the native IgM purified from tilapia serum, the specific peptides in the heavy chains of each IgM could be detected. At the transcriptional level, Igh mu 1 and Igh mu 2 had similar expression patterns, and both were constitutively expressed in both systemic and mucosal lymphoid tissues. Detection of the expression of Igh mu 1 and Igh mu 2 in single IgM+ B cells sorted by flow cytometry revealed that Igh mu 1 and Igh mu 2 are mainly co-expressed by single IgM+ B cells. After Poly I:C, Aeromonas hydrophila or Streptococcus agalactiae treatment, Igh mu 1 and Igh mu 2 exhibited different expression profiles, the transcriptional level of Igh mu 1 mainly involved in bacterial infection while Igh mu 2 was more related to Poly I:C stimulation. These data from the genome, transcription, and protein levels have demonstrated that there exist two subclasses of IgM in tilapia, and that their heavy chain genes display different expression patterns during stimulation. Overall, our data reflect the diversity and complexity of IgM in tilapia, thus provide a better understanding of the IgM evolution in teleost fish.
Keyword ：
Expression Expression IgM IgM Nile tilapia Nile tilapia Subclass Subclass
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| GB/T 7714 | Yao, Yuan-Yuan , Pan, Chen-Xi , Hao, Yu-Dong et al. Discovery of two IgM subclasses in Nile tilapia (Oreochromis niloticus) provides insights into IgM evolution in teleost fish [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
| MLA | Yao, Yuan-Yuan et al. "Discovery of two IgM subclasses in Nile tilapia (Oreochromis niloticus) provides insights into IgM evolution in teleost fish" . | FISH & SHELLFISH IMMUNOLOGY 166 (2025) . |
| APA | Yao, Yuan-Yuan , Pan, Chen-Xi , Hao, Yu-Dong , Liu, Xun , Chen, Dan-Dan , Cui, Zheng-Wei et al. Discovery of two IgM subclasses in Nile tilapia (Oreochromis niloticus) provides insights into IgM evolution in teleost fish . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
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The transcription factor Tcf3 is a key regulator during mammalian B cell development and activation, which is highly expressed in precursor B cells, downregulated in mature B cells, and re-expressed upon B cell activation. Despite the extensive studies in mammals, its role in teleost B cells remains poorly characterized. Here, using the economically important marine teleost large yellow croaker (Larimichthys crocea) as a model, we identified two Tcf3 homologs (LcTcf3a and LcTcf3b) and found that both genes were upregulated during B cell activation. By cloning the predicted promoter region and serial truncation, we found that the core promoter regions of LcTcf3a and LcTcf3b were located about 600 bp around the transcription start sites. Interestingly, while both of the two gene promoters contain CpG islands, bisulfite sequencing showed constitutive hypomethylation both before and after B cell activation. However, in vitro methylation suppressed the promoter activities of LcTcf3a and LcTcf3b, indicating their DNA methylation-dependent regulation. Together, these results suggested a "poised" state of LcTcf3a and LcTcf3b in resting B cells, where LcTcf3a and LcTcf3b were repressed but preparing for fast upregulation during immune response. Our findings proposed that LcTcf3a and LcTcf3b likely contribute to B cell activation in a teleost model and their promoter activities may be regulated by DNA methylation, thus providing insights into the understanding of the teleost B cell immunity.
Keyword ：
DNA methylation DNA methylation IgM plus B cells IgM plus B cells Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) Tcf3 Tcf3
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| GB/T 7714 | Chen, Qiuxuan , Zhu, Zhuo , Li, Xinran et al. Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM plus B cell activation in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
| MLA | Chen, Qiuxuan et al. "Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM plus B cell activation in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 166 (2025) . |
| APA | Chen, Qiuxuan , Zhu, Zhuo , Li, Xinran , Lai, Guolong , Cui, Zhengwei , Chen, Xinhua et al. Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM plus B cell activation in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
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Spleen tyrosine kinase (SYK), a non-receptor protein tyrosine kinase, is a key component of B cell receptor signaling and can regulate multiple physiological functions of B cells in mammals. In this study, a SYK gene was cloned and characterized from large yellow croaker (Larimichthys crocea) (LcSYK), whose open reading frame consists of 1851 base pairs and encodes 616 amino acid residues. The predicted LcSYK protein contains two Nterminal tandem Src homology 2 domains and a C-terminal tyrosine kinase catalytic domain, and shares a high amino acid sequence identity with SYK sequences in other vertebrate species. LcSYK was mainly expressed in immune tissues, such as head kidney, trunk kidney, spleen, and gill. The mRNA expression of LcSYK in primary head kidney leukocytes was not changed at 12, 24, and 48 h after lipopolysaccharide (LPS) stimulation. LPS stimulation upregulated the mRNA expression and protein production of IgM in IgM+ B cells, accompanied by an increase in the phosphorylation level, but not the total protein level, of LcSYK. Moreover, when we used PRT062607 HCl to inhibit the phosphorylation of LcSYK, both mRNA expression and protein production of IgM in IgM+ B cells were significantly suppressed. These results suggest that SYK phosphorylation may play a role in LPS-induced IgM production by IgM+ B cells, improving our understanding of the role of SYK in immunoglobulin responses of B cells in fish.
Keyword ：
B cell B cell Immunoglobulin Immunoglobulin Large yellow croaker Large yellow croaker SYK SYK
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| GB/T 7714 | Xie, Hongjun , Cao, Shuangshuang , Chen, Yueming et al. The role of SYK phosphorylation in LPS-induced immunoglobulin responses of B cells in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
| MLA | Xie, Hongjun et al. "The role of SYK phosphorylation in LPS-induced immunoglobulin responses of B cells in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 161 (2025) . |
| APA | Xie, Hongjun , Cao, Shuangshuang , Chen, Yueming , Wang, Zhiqiang , Chen, Xinhua , Cui, Zhengwei . The role of SYK phosphorylation in LPS-induced immunoglobulin responses of B cells in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 161 . |
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Mucosal immunity in mucosa-associated lymphoid tissues (MALTs) plays crucial roles in resisting infection by pathogens, including parasites, bacteria and viruses. However, the mucosal immune response in the MALTs of large yellow croaker (Larimichthys crocea) upon parasitic infection remains largely unknown. In this study, we investigated the role of B cells and T cells in the MALTs of large yellow croaker following Cryptocaryon irritans infection. Upon C. irritans infection, the total IgM and IgT antibody levels were significantly increased in the skin mucus and gill mucus. Notably, parasite-specific IgM antibody level was increased in the serum, skin and gill mucus following parasitic infection, while the level of parasite-specific IgT antibody was exclusively increased in MALTs. Moreover, parasitic infection induced both local and systemic aggregation and proliferation of IgM+ B cells, suggesting that the increased levels of IgM in mucus may be derived from both systemic and mucosal immune tissues. In addition, we observed significant aggregation and proliferation of T cells in the gill, head kidney and spleen, suggesting that T cells may also be involved in the systemic and mucosal immune responses upon parasitic infection. Overall, our findings provided further insights into the role of immunoglobulins against pathogenic infection, and the simultaneous aggregation and proliferation of both B cells and T cells at mucosal surfaces suggested potential interactions between these two major lymphocyte populations during parasitic infection.
Keyword ：
B cells B cells Cryptocaryon irritans Cryptocaryon irritans Immunoglobulins Immunoglobulins Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea ) T cells T cells
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| GB/T 7714 | Ding, Yangyang , Zhang, Yameng , Shen, Yibo et al. Aggregation and proliferation of B cells and T cells in MALTs upon Cryptocaryon irritans infection in large yellow croaker Larimichthys crocea [J]. | FISH & SHELLFISH IMMUNOLOGY , 2024 , 149 . |
| MLA | Ding, Yangyang et al. "Aggregation and proliferation of B cells and T cells in MALTs upon Cryptocaryon irritans infection in large yellow croaker Larimichthys crocea" . | FISH & SHELLFISH IMMUNOLOGY 149 (2024) . |
| APA | Ding, Yangyang , Zhang, Yameng , Shen, Yibo , Zhang, Yihan , Li, Zhangqi , Shi, Yuan et al. Aggregation and proliferation of B cells and T cells in MALTs upon Cryptocaryon irritans infection in large yellow croaker Larimichthys crocea . | FISH & SHELLFISH IMMUNOLOGY , 2024 , 149 . |
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Fc受体(Fc Receptors, FcRs)是一类与免疫球蛋白的Fc段特异性结合，介导多种免疫反应的受体分子。本研究克隆得到了大黄鱼(Larimichthys crocea)6个FcRs基因，分别是LcFcγRⅠL、LcFcγRⅡ、LcFcγRⅡaL、LcFcγRⅢ、LcFcRL5和LcFcRLA,其开放阅读框(ORF)大小分别为1 070、2 486、1 145、 1 955、4 125、1 659 bp。大黄鱼的6个LcFcRs都具有一个信号肽和2～8个免疫球蛋白(Ig)结构域，LcFcγRⅡ、LcFcγRⅢ和LcFcRL5还包含一个跨膜区。实时荧光定量PCR结果显示LcFcγRⅠL、LcFcγRⅡ、LcFcγRⅡaL和LcFcγRⅢ主要在鳃或肠等黏膜组织中表达，而LcFcRL5和LcFcRLA主要在脾和头肾等系统免疫组织中表达；革兰氏阴性细菌细胞壁脂多糖(LPS)刺激后，这6个LcFcRs基因头肾和脾脏中的表达在不同时间出现了显著上调；双链RNA病毒类似物Poly(I:C)刺激后，6个LcFcRs在头肾和脾脏中的表达出现不同程度的上调，而LcFcRL5在脾脏中的表达显著上调，在头肾中则明显下调，提示大黄鱼FcRs可能在LPS和Poly(I:C)诱导的免疫反应中起着重要但又不同的作用。
Keyword ：
FcRs FcRs 免疫球蛋白 免疫球蛋白 分子克隆 分子克隆 大黄鱼 大黄鱼 海洋生物学 海洋生物学
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| GB/T 7714 | 杨玉生 , 程安怡 , 张亚萌 et al. 大黄鱼FcRs基因的克隆及其在免疫应答中的初步功能分析 [J]. | 应用海洋学学报 , 2024 , 43 (01) : 160-170 . |
| MLA | 杨玉生 et al. "大黄鱼FcRs基因的克隆及其在免疫应答中的初步功能分析" . | 应用海洋学学报 43 . 01 (2024) : 160-170 . |
| APA | 杨玉生 , 程安怡 , 张亚萌 , 崔正伟 , 陈新华 . 大黄鱼FcRs基因的克隆及其在免疫应答中的初步功能分析 . | 应用海洋学学报 , 2024 , 43 (01) , 160-170 . |
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As the predominant immunoglobulin (Ig) isotype, IgM plays a crucial role in the acquired immunity of verte-brates. There is only one Ig mu gene in mammals, except cattle, while the number of Ig mu gene varies among teleost fish. In the current study, we found two functional Ig mu genes (Ig mu 1 and Ig mu 2) and a pseudo C mu gene (psi Ig mu) in large yellow croaker (Larimichthys crocea). Both Ig mu 1 and Ig mu 2 genes possessed two transcript variants, which encoded the heavy chains of secreted (sIgM1 and sIgM2) and membrane-bound IgM1 and IgM2 (mIgM1 and mIgM2), respectively. Both the heavy chains of sIgM1 and sIgM2 consisted of a variable Ig domain, four constant Ig domains (CH1, CH2, CH3 and CH4) and a secretory tail, while those of mIgM1 and mIgM2 consisted of a variable Ig domain, three constant Ig domains (CH1, CH2 and CH3), a transmembrane domain and a short cytoplasmic tail. Cysteine residues that are necessary for the formation of intrachain and interchain disulfide bonds and tryptophan residues that are important for the folding of the Ig superfamily domain were well conserved in large yellow croaker IgM1 and IgM2. Interestingly, large yellow croaker IgM2 had an extra cysteine (C94) in the CH1 domain compared with IgM1, which may cause the structural difference between IgM1 and IgM2. A liquid chromatography-tandem mass spectrometry analysis revealed that both IgM1 and IgM2 were present at the protein level in large yellow croaker serum. Both the Ig mu 1 and Ig mu 2 genes were mainly expressed in systemic immune tissues, such as head kidney and spleen, but the expression level of Ig mu 2 was much lower than that of Ig mu 1. After Pseudomonas plecoglossicida infection, the expression levels of Ig mu 1 and Ig mu 2 in both the spleen and head kidney were significantly upregulated, with a higher upregulation of Ig mu 2 than that of Ig mu 1. These results suggested that Ig mu 1 and Ig mu 2 may play a differential role in the immune response of large yellow croaker against bacterial infection.
Keyword ：
Bacterial infection Bacterial infection IgM IgM Large yellow croaker Large yellow croaker Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Cui, Zhengwei , Zhao, Han , Chen, Xinhua . Molecular and functional characterization of two IgM subclasses in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2023 , 134 . |
| MLA | Cui, Zhengwei et al. "Molecular and functional characterization of two IgM subclasses in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 134 (2023) . |
| APA | Cui, Zhengwei , Zhao, Han , Chen, Xinhua . Molecular and functional characterization of two IgM subclasses in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2023 , 134 . |
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Large yellow croaker (Larimichthys crocea), an economically important marine fish in China, has suffered from serious vibriosis, which has resulted in great economic losses for the large yellow croaker industry. Vaccination has been considered to be a safe and effective method to prevent and control vibriosis. However, due to the complex diversity and serotypes of the Vibrio genus, the progress of Vibrio vaccine development is still slow. In this study, we prepared recombinant Vibrio dihydrolipoamide dehydrogenase (rDLD) protein and investigated its potential as a candidate to be a subunit vaccine against Vibrio. The lysozyme activity and the rDLD-specific antibody level in sera of large yellow croakers immunized with rDLD were significantly higher than those in the control group, and the transcript levels of proinflammatory cytokines (IL-6, IL-8, IL-1 beta), MHC II alpha/beta, CD40, CD8 alpha, IL-4/13A, and IL-4/13B were significantly up-regulated in the spleen and head kidney of large yellow croakers immunized with rDLD, suggesting that rDLD could induce both specific and nonspecific immune responses in this species. In addition, rDLD protein increased the survival rate of large yellow croakers against Vibrio alginolyticus and Vibrio parahaemolyticus, with the relative percent of survival (RPS) being 74.5% and 66.9%, respectively. These results will facilitate the development of a potential subunit vaccine against Vibrio in large yellow croaker aquaculture.
Keyword ：
dihydrolipoamide dehydrogenase dihydrolipoamide dehydrogenase Larimichthys crocea Larimichthys crocea relative percent survival rate relative percent survival rate subunit vaccine subunit vaccine Vibrio Vibrio
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| GB/T 7714 | Li, Xiaomeng , Tan, Yuanzhen , Zhang, Zheng et al. Development of Recombinant Dihydrolipoamide Dehydrogenase Subunit Vaccine against Vibrio Infection in Large Yellow Croaker [J]. | FISHES , 2022 , 7 (1) . |
| MLA | Li, Xiaomeng et al. "Development of Recombinant Dihydrolipoamide Dehydrogenase Subunit Vaccine against Vibrio Infection in Large Yellow Croaker" . | FISHES 7 . 1 (2022) . |
| APA | Li, Xiaomeng , Tan, Yuanzhen , Zhang, Zheng , Huang, Yupeng , Mu, Pengfei , Cui, Zhengwei et al. Development of Recombinant Dihydrolipoamide Dehydrogenase Subunit Vaccine against Vibrio Infection in Large Yellow Croaker . | FISHES , 2022 , 7 (1) . |
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