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学者姓名：黄小红
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Abstract ：
Streptococcus suis (S. suis) is considered as one of the most crucial bacterial pathogens that leads to serious economic losses to the swine industry. Different S. suis serotypes exhibit diverse characteristics in population structure and pathogenicity. Epidemiology data underscore the importance of S. suis serotype 3 (SS3). However, except for a few epidemiological information, limited study information is available on this serotype. Herein, a pathogenic SS3 (the S. suis strain YA) was isolated from infected piglets in clinical practice, and then whole genome sequencing and analysis, hemolytic activity, antimicrobial susceptibility, pathogenicity to mice and piglets were conducted. The results of the whole genome sequencing of the S. suis strain YA showed that the complete genome was 2,167,682 bp in length with a G + C content of 41.2% and exhibited a unique sequence type (ST1801). The result of phylogenetic tree showed that it was most closely related to strain DNC15 and 6407 (ST54) from Denmark. The tet(W) and erm(B) resistant genes were identified in the S. suis strain YA by inserting into rum locus, in accordance with the result of resistance to tetracyclines and macrolide-lincosamide-streptogramin antibiotics. Twenty-seven key virulence factors were detected in the S. suis strain YA, including sly, ef and mrp, which contribute to pathogenicity in mice and piglets, causing bleeding and congestion in multiple tissue organs especially in the brains. And the LD50 value for mice was 1.54 x 107 CFU. Therefore, our research emphasizes the importance of understanding SS3, and provides valuable information for the scientific prevention and control of S. suis.
Keyword ：
pathogenic pathogenic sequence analysis sequence analysis Streptococcus suis serotype 3 Streptococcus suis serotype 3 virulence factors virulence factors
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| GB/T 7714 | Wang, Longbai , Qiu, Jingli , He, Bing et al. Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of Streptococcus suis Serotype 3 [J]. | PATHOGENS , 2025 , 14 (2) . |
| MLA | Wang, Longbai et al. "Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of Streptococcus suis Serotype 3" . | PATHOGENS 14 . 2 (2025) . |
| APA | Wang, Longbai , Qiu, Jingli , He, Bing , Wu, Xuemin , Chen, Qiuyong , Wang, Quanxi et al. Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of Streptococcus suis Serotype 3 . | PATHOGENS , 2025 , 14 (2) . |
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Oxidative stress is intricately associated with a variety of chicken diseases, and represents a significant challenge within the poultry industry. Baicalin (BA), a compound extracted from the plant Scutellaria Baicalensis, possesses potent antioxidant properties, however, the effects and mechanisms underlying the antioxidant activity of BA in chickens remain to be fully elucidated. This study aimed to demonstrate the antioxidant effects of BA in vitro, and to explore the underlying antioxidant mechanism. In this study, hydrogen peroxide (H2O2) was used to construct a model of oxidative stress in DF-1 cell line, and the antioxidant effect of BA in DF-1 cells was detected. The results indicated that BA significantly ameliorated the decline in total antioxidant capacity of DF-1 cells induced by H2O2, and preserved the mitochondrial function of DF-1 cells. Concurrently, H2O2 induced abnormal enrichment of mitochondria & hybull;associated membranes (MAMs) in DF-1 cells, leading to mitochondrial Ca2+ overload, and BA significantly mitigated this effect. Furthermore, transcriptomic sequencing and western blot analysis suggested that the Mitofusin-2 (Mfn2) gene is involved in the antioxidant process of BA. Moreover, following the siRNA-mediated interference of the Mfn2 gene, the antioxidant efficacy of BA was observed to be significantly diminished. In conclusion, BA alleviates H2O2-induced oxidative stress in DF-1 cells via the Mfn2/ MAMs/Ca2+ pathway.
Keyword ：
Baicalin Baicalin Ca2+ Ca2+ MAM MAM Mfn2 Mfn2 Oxidative stress Oxidative stress
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| GB/T 7714 | Lin, Zhaoyan , Wang, Jiao , Li, Junxin et al. Baicalin alleviates oxidative stress in DF-1 cell line via the Mfn2/MAMs/Ca (2+) pathway [J]. | POULTRY SCIENCE , 2025 , 104 (11) . |
| MLA | Lin, Zhaoyan et al. "Baicalin alleviates oxidative stress in DF-1 cell line via the Mfn2/MAMs/Ca (2+) pathway" . | POULTRY SCIENCE 104 . 11 (2025) . |
| APA | Lin, Zhaoyan , Wang, Jiao , Li, Junxin , Zheng, Yu , Zheng, Bohan , Li, Qinjin et al. Baicalin alleviates oxidative stress in DF-1 cell line via the Mfn2/MAMs/Ca (2+) pathway . | POULTRY SCIENCE , 2025 , 104 (11) . |
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Keyword ：
curcumin curcumin drug combination drug combination IGF-1/PI3K/AKT/mTOR pathway IGF-1/PI3K/AKT/mTOR pathway oxidative stress oxidative stress selenium selenium
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| GB/T 7714 | He, Zixuan , Lin, Zhaoyan , Yan, Ye et al. Curcumin and selenium synergistically mitigate oxidative stress in white-feathered broilers (vol 12, 1600466, 2025) [J]. | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
| MLA | He, Zixuan et al. "Curcumin and selenium synergistically mitigate oxidative stress in white-feathered broilers (vol 12, 1600466, 2025)" . | FRONTIERS IN VETERINARY SCIENCE 12 (2025) . |
| APA | He, Zixuan , Lin, Zhaoyan , Yan, Ye , Wang, Jiao , Zhang, Shizhong , Zheng, Bohan et al. Curcumin and selenium synergistically mitigate oxidative stress in white-feathered broilers (vol 12, 1600466, 2025) . | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
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Oxidative stress is closely associated with diarrhea in piglets, and alleviating intestinal oxidative stress may emerge as an effective strategy for porcine diarrhea. Curcumin (Cur) and selenium (Se) are both well recognized for their potent antioxidant effects. This study established in vitro (IPEC-J2 cells) and in vivo (ICR mice) intestinal oxidative stress models to investigate the effects and mechanisms of Cur combined with Se in alleviating oxidative stress. The results showed that Cur and Se exhibited synergistic antioxidant effects in vitro, outperforming individual treatments. Additionally, pretreatment with Cur and Se significantly attenuated dextran sulfate sodium (DSS)-induced colitis in ICR mice and improved serum antioxidant indices. Specifically, the mRNA levels of Nrf2, HO-1, and NQO-1 were upregulated, while the mRNA levels of NF-kappa B, IL-1 beta, and TNF-alpha were downregulated in colonic tissues. Finally, the 16S rRNA sequencing showed that DSS reduced alpha diversity and increased Simpson indices, while Cur and Se restored diversity indices and normalized beta diversity. To summarize, Cur and Se synergistically alleviate oxidative stress in IPEC-J2 cells and ICR mice, demonstrating therapeutic potential for intestinal disorders in pigs.
Keyword ：
curcumin curcumin gut microbiota gut microbiota IPEC-J2 IPEC-J2 oxidative stress oxidative stress selenium selenium
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| GB/T 7714 | Zheng, Yu , Liu, Jiali , Li, Junxin et al. Curcumin and Selenium Synergistically Alleviate Oxidative Stress in IPEC-J2 Cells and ICR Mice [J]. | BIOLOGY-BASEL , 2025 , 14 (9) . |
| MLA | Zheng, Yu et al. "Curcumin and Selenium Synergistically Alleviate Oxidative Stress in IPEC-J2 Cells and ICR Mice" . | BIOLOGY-BASEL 14 . 9 (2025) . |
| APA | Zheng, Yu , Liu, Jiali , Li, Junxin , Zheng, Bohan , Li, Qinjin , Huang, Xiaohong et al. Curcumin and Selenium Synergistically Alleviate Oxidative Stress in IPEC-J2 Cells and ICR Mice . | BIOLOGY-BASEL , 2025 , 14 (9) . |
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【目的】探究猴头菇多糖（HEP）对大黄鱼原代头肾巨噬细胞（PKM）的免疫调节作用，为HEP在鱼类养殖过程中的推广应用提供依据。【方法】采用Percoll密度梯度离心技术分离大黄鱼PKM。用50、100、200、400、800μg·mL
Keyword ：
促炎细胞因子 促炎细胞因子 大黄鱼 大黄鱼 头肾巨噬细胞 头肾巨噬细胞 活性氧 活性氧 猴头菇多糖 猴头菇多糖
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| GB/T 7714 | 安慧敏 , 王馨怡 , 王永阳 et al. 猴头菇多糖对大黄鱼原代头肾巨噬细胞的免疫调节作用 [J]. | 福建农林大学学报(自然科学版) , 2025 , 54 (05) : 630-639 . |
| MLA | 安慧敏 et al. "猴头菇多糖对大黄鱼原代头肾巨噬细胞的免疫调节作用" . | 福建农林大学学报(自然科学版) 54 . 05 (2025) : 630-639 . |
| APA | 安慧敏 , 王馨怡 , 王永阳 , 黄小红 , 张伟妮 . 猴头菇多糖对大黄鱼原代头肾巨噬细胞的免疫调节作用 . | 福建农林大学学报(自然科学版) , 2025 , 54 (05) , 630-639 . |
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The objective of this research was to investigate the efficacy of lotus leaf in the prevention and treatment of inflammatory diarrhea in pigs, utilizing network pharmacology and in vitro methodologies. Initially, LC-MS was employed to analyze the constituents of lotus leaf extract (LLE); then, the TCMSP database was utilized to identify the active components and their corresponding targets. The GeneCards database was consulted to identify disease-related targets pertinent to inflammatory diarrhea in pigs. A drug ingredient-disease target network was constructed using Cytoscape software. Subsequently, the STRING database facilitated protein interaction analysis, which was also visualized through Cytoscape. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were conducted based on the genes shared between disease and LLE targets. Molecular docking of the active ingredients with key targets was performed using Autodock Vina. Subsequently, an in vitro LPS-induced inflammation model was established using IPEC-J2 cells to validate the predictions made through network pharmacology. Verification was conducted via flow cytometry and Western blot analysis. The LC-MS assay and TCMSP retrieval results revealed that Quercetin, Nuciferine, Kaempferol, Leucodelphinidin, and Catechin were identified as the main compounds of LLE, associated with 181 potential targets. A total of 5995 targets were linked to inflammatory diarrhea in pigs, with 159 overlapping targets identified between the bioactive compounds and the disease. Notable key targets included TNF-alpha, IL-6, caspase-3, TP53, and AKT, which are integral to inflammation and apoptosis processes. GO functional annotation indicated significant enrichment in biological processes such as gene expression regulation and transcription from RNA polymerase II promoters. KEGG pathway analysis highlighted critical pathways, including TNF signaling and apoptosis. Furthermore, molecular docking analyses demonstrated that the bioactive components of lotus leaf exhibited a strong binding affinity for essential targets, including AKT1, BAX, caspase-3, CCL2, IL-6, IL-10, MPK14, NOS3, PTGS1, and TNF-alpha. In vitro experiments confirmed that LLE significantly inhibited LPS-induced apoptosis in IPEC-J2 cells and suppressed the activation of the TNF-alpha-mediated apoptosis pathway. This study offers novel insights into the therapeutic potential of Chinese medicine and its constituents in addressing inflammatory diarrhea in pigs.
Keyword ：
apoptosis apoptosis inflammatory diarrhea in pigs inflammatory diarrhea in pigs lotus leaf lotus leaf molecular docking molecular docking network pharmacology network pharmacology
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| GB/T 7714 | Zheng, Yu , Zheng, Jiana , Wang, Jiao et al. Network Pharmacology and In Vitro Experimental Validation Reveal the Anti-Inflammatory and Anti-Apoptotic Effects of Lotus Leaf Extract in Treating Inflammatory Diarrhea in Pigs [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (5) . |
| MLA | Zheng, Yu et al. "Network Pharmacology and In Vitro Experimental Validation Reveal the Anti-Inflammatory and Anti-Apoptotic Effects of Lotus Leaf Extract in Treating Inflammatory Diarrhea in Pigs" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 47 . 5 (2025) . |
| APA | Zheng, Yu , Zheng, Jiana , Wang, Jiao , Li, Junxin , Liu, Jiali , Zheng, Bohan et al. Network Pharmacology and In Vitro Experimental Validation Reveal the Anti-Inflammatory and Anti-Apoptotic Effects of Lotus Leaf Extract in Treating Inflammatory Diarrhea in Pigs . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (5) . |
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在当前的饲料“禁抗”大背景下，以植物源活性成分作为饲料添加剂已成为鱼类养殖业的研究热点。姜黄素作为植物源多酚类化合物，具有抗菌、抗氧化及抗炎等多种生理功能，是一种值得开发的新型绿色鱼类饲料添加剂。本文综述了姜黄素的理化性质及其对鱼类生产性状、抗氧化、脂质代谢及肠道菌群的影响，以期为姜黄素作为新型饲料添加剂及抗生素替代品应用于鱼类养殖提供参考，促进水产养殖行业的可持续发展。
Keyword ：
姜黄素 姜黄素 水产养殖 水产养殖 生理功能 生理功能 饲料添加剂 饲料添加剂
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| GB/T 7714 | 罗诗怡 , 黄小红 , 严美姣 . 姜黄素在鱼类养殖中的应用研究进展 [J]. | 动物营养学报 , 2025 , 37 (02) : 794-803 . |
| MLA | 罗诗怡 et al. "姜黄素在鱼类养殖中的应用研究进展" . | 动物营养学报 37 . 02 (2025) : 794-803 . |
| APA | 罗诗怡 , 黄小红 , 严美姣 . 姜黄素在鱼类养殖中的应用研究进展 . | 动物营养学报 , 2025 , 37 (02) , 794-803 . |
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Introduction Oxidative stress is closely linked to various diseases in chickens, representing an urgent concern that needs to be addressed in the poultry industry. Curcumin (CUR) and selenium (Se) are both recognized for their great antioxidant effects, however, the combination use of them in broilers has not been reported. This study aims to demonstrate the synergistic antioxidant effects of CUR and Se in vitro and in vivo, and to explore the underlying antioxidant mechanism.Methods The experiments were conducted on the DF-1 cell line and 400 healthy male White-feathered Broilers, day old, weighing 43.89 +/- 0.70 g. Hydrogen peroxide (H2O2) and dexamethasone (Dex) were used to conduct oxidative stress model.Results The results demonstrated that CUR and Se synergistically enhanced the antioxidant capacity of DF-1 cells, with a combination index (CI) less than 1; next, CUR and Se increased the total antioxidant capacity and superoxide dismutase (SOD) activity, decreased lactate dehydrogenase (LDH) and malondialdehyde (MDA) levels in broiler liver and heart tissues, alleviated Dex-induced liver and heart injury and liver cell apoptosis in broilers; moreover, the protein expression of IGF-1, PI3K-p110 beta, phosphorylated AKT and phosphorylated mTOR in liver and heart tissues were increased after the combination treatment.Discussion In conclusion, CUR and Se alleviate oxidative stress in White-feathered Broilers synergistically, and the synergistic antioxidant effects are related to IGF-1/PI3K/AKT/mTOR pathway.
Keyword ：
curcumin curcumin drug combination drug combination IGF-1/PI3K/AKT/mTOR pathway IGF-1/PI3K/AKT/mTOR pathway oxidative stress oxidative stress selenium selenium
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| GB/T 7714 | He, Zixuan , Lin, Zhaoyan , Yan, Ye et al. Curcumin and selenium synergistically mitigate oxidative stress in white-feathered broilers [J]. | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
| MLA | He, Zixuan et al. "Curcumin and selenium synergistically mitigate oxidative stress in white-feathered broilers" . | FRONTIERS IN VETERINARY SCIENCE 12 (2025) . |
| APA | He, Zixuan , Lin, Zhaoyan , Yan, Ye , Wang, Jiao , Zhang, Shizhong , Zheng, Bohan et al. Curcumin and selenium synergistically mitigate oxidative stress in white-feathered broilers . | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
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以玉米DDGS替代饲料中不同比例[0(D0)、25%(D25)、50%(D50)和75%(D75)]的豆粕蛋白,配制成玉米DDGS含量分别为0、13.63%、27.26%和40.89%的4种等氮等能饲料,饲喂初始体重为(3.60±0.01) g的中华圆田螺(Cipangopaludina cathayensis)幼螺56d,探讨其对中华圆田螺幼螺生长性能、体成分、抗氧化能力和消化系统组织结构的影响。结果显示:(1) D25组终末体重(FBW)、特定生长率(SGR)和增重率(WGR)显著高于D0组(P<0.05), D25和D0组之间存活率(SR)、摄食率(FR)、饲料效率(FE)和蛋白质效率(PER)无显著差异(P>0.05),但D50和D75组的FBW、SGR和WGR显著低于D0组(P<0.05); D25组中华圆田螺幼螺螺体粗蛋白、粗脂肪和灰分含量均显著高于D0组(P<0.05); D50和D75组中华圆田螺幼螺肝胰腺和肠道的胰蛋白酶(TRY)及脂肪酶(LPS)的活性均显著低于D0组(P<0.05),而各组间淀粉酶(AMS)活性无显著差异(P>0.05)。(2) D25组中华圆田螺幼螺肝胰腺和肠道碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽S转移酶(GST)活性与D0组相比均无显著差异(P>0.05), D50和D75组中华圆田螺幼螺肝胰腺和肠道CAT、SOD和GPx活性显著低于D0组(P<0.05), D75组肝胰腺和肠道丙二醛(MDA)含量显著高于D0组(P<0.05); D50和D75组肝胰腺GPx、GST和SOD和肠道GST基因表达量均显著低于D0组(P<0.05)。(3) D25组中华圆田螺幼螺肝胰腺和肠道组织结构与D0组无显著差异, D75组中华圆田螺幼螺肝胰腺部分肝小管结构紊乱,基膜破裂; D50和D75组中华圆田螺幼螺肠道上皮细胞受损,固有层萎缩。综上结果,玉米DDGS在中华圆田螺幼螺饲料中的添加比例以不超过13.63%为宜。
Keyword ：
中华圆田螺 中华圆田螺 抗氧化能力 抗氧化能力 玉米DDGS 玉米DDGS 生长性能 生长性能 蛋白源替代 蛋白源替代
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| GB/T 7714 | 黄莹 , 刘轩宇 , 张雯怡 et al. 玉米DDGS替代豆粕对中华圆田螺幼螺生长性能、抗氧化能力和消化系统组织结构的影响 [J]. | 水生生物学报 , 2025 , 49 (07) : 135-147 . |
| MLA | 黄莹 et al. "玉米DDGS替代豆粕对中华圆田螺幼螺生长性能、抗氧化能力和消化系统组织结构的影响" . | 水生生物学报 49 . 07 (2025) : 135-147 . |
| APA | 黄莹 , 刘轩宇 , 张雯怡 , 廖梦香 , 林学文 , 朱晓鸣 et al. 玉米DDGS替代豆粕对中华圆田螺幼螺生长性能、抗氧化能力和消化系统组织结构的影响 . | 水生生物学报 , 2025 , 49 (07) , 135-147 . |
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旨在以杜洛克公猪精液为研究对象，通过N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)抑制剂体外处理精液，经12.5%SDS-PAGE对处理好的精子样品进行双向电泳分离，筛选NAGase作用互作点蛋白表达差异；表达丰度差异的蛋白经酶消化后进行质谱分析。结果：银染筛选获得57个差异表达蛋白点在凝胶中，蛋白点主要分布在pH值3～10和相对分子质量14.4～116 kDa的范围内；经NCBI和Mascot数据库进行差异表达蛋白匹配搜索，成功鉴定与精子功能相关的上调差异表达蛋白8个，分别是磷脂酰乙醇胺结合蛋白4(PEBP4)、精卵融合蛋白4(IZUMO4)、精浆糖蛋白1(PSP-1)、猪精子黏附蛋白(AWN)、糖结合蛋白(SPMI)、细胞色素c氧化酶Ⅵα亚基多肽1(COX6A1)、二氢硫辛酸脱氢酶蛋白(DLD)和伴侣蛋白1(TCP1);8个上调差异表达蛋白主要涉及精子成熟、精子运动、精卵识别等生理功能。通过STRING工具分析蛋白质互作关系表明IZUMO蛋白可能是NAGase调节精子功能的一个重要靶点并形成了可能的蛋白质调控网络。研究结果为提高公猪生产性能研究奠定了基础。
Keyword ：
N-乙酰-β-D-氨基葡萄糖苷酶 N-乙酰-β-D-氨基葡萄糖苷酶 杜洛克公猪 杜洛克公猪 精子功能 精子功能 蛋白质组学 蛋白质组学
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| GB/T 7714 | 林秋敏 , 赖宝色 , 钟佩云 et al. 杜洛克公猪精液NAGase对调控精子功能蛋白质组学的研究 [J]. | 畜牧与兽医 , 2025 , 57 (02) : 14-21 . |
| MLA | 林秋敏 et al. "杜洛克公猪精液NAGase对调控精子功能蛋白质组学的研究" . | 畜牧与兽医 57 . 02 (2025) : 14-21 . |
| APA | 林秋敏 , 赖宝色 , 钟佩云 , 林祚贵 , 徐磊 , 黄小红 . 杜洛克公猪精液NAGase对调控精子功能蛋白质组学的研究 . | 畜牧与兽医 , 2025 , 57 (02) , 14-21 . |
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