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学者姓名：敖敬群
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Abstract ：
Signal transducer and activator of transcription (STAT) family members, which serve as transcription activators, play a central role in regulating inflammatory responses. While STATs have been well-characterized in mammals, systematic studies in fish remain scarce. In the present study, eight STATs in Cromileptes altivelis-CaSTAT1a, CaSTAT1b, CaSTAT2, CaSTAT3, CaSTAT4, CaSTAT5a, CaSTAT5b, and CaSTAT6-were characterized using PCR cloning and bioinformatics approaches. Phylogenetic analysis indicated that STATs have undergone significant evolutionary conservation throughout their lineage. Tissue expression patterns in healthy fish revealed that the eight CaSTATs displayed spatiotemporal specificity, with greater expression observed in gill, blood, and muscle. In response to bacterial (Vibrio harveyi, Streptococcus agalactiae) or viral (nervous necrosis virus, NNV) challenge, CaSTATs were robustly expressed and exhibited pathogen-, tissue-, and time-dependent expression divergence. In summary, our data highlight the indispensable roles of eight CaSTATs in modulating host immune responses.
Keyword ：
Cromileptes altivelis Cromileptes altivelis Expression patterns Expression patterns Immune defense Immune defense Pathogens challenge Pathogens challenge STAT STAT
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| GB/T 7714 | Zhang, Panpan , Lin, Shuyi , Xie, Zixin et al. Pathogen-driven modulation of eight STATs in Cromileptes altivelis: Comparative analysis from homeostasis to immune activation [J]. | FISH & SHELLFISH IMMUNOLOGY , 2026 , 168 . |
| MLA | Zhang, Panpan et al. "Pathogen-driven modulation of eight STATs in Cromileptes altivelis: Comparative analysis from homeostasis to immune activation" . | FISH & SHELLFISH IMMUNOLOGY 168 (2026) . |
| APA | Zhang, Panpan , Lin, Shuyi , Xie, Zixin , Chen, Guisen , Cao, Zhenjie , Zhang, Chen et al. Pathogen-driven modulation of eight STATs in Cromileptes altivelis: Comparative analysis from homeostasis to immune activation . | FISH & SHELLFISH IMMUNOLOGY , 2026 , 168 . |
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Pseudomonas plecoglossicida is a pathogen bacterium responsible for visceral white spot disease (VWND) in Large Yellow Croakers (Larimichthys crocea), causing significant economic losses in commercial fish farms. The prolinealanine-alanine-arginine repeats protein (PAAR) is a core component of the spike structure in the type VI secretion system (T6SS), which injects toxic effectors into host and contributes to bacterial virulence. However, the role of the PAAR gene in P. plecoglossicida and its impact on bacterial infection and host immune responses remain unexplored. In this study, PAAR-1 was identified for the first time in P. plecoglossicida as an effector gene within the T6SS-1 gene cluster, which is regulated and secreted by T6SS-1. The P. plecoglossicida mutant strain (z.PAAR-1) and its complementary strain (C-z.PAAR-1) were constructed for subsequent investigation. Compared to the wild-type strain, z.PAAR-1 exhibited reduced biofilm formation, adhesion, total antioxidant capacity, and secretion of T6SS core protein Hcp-1. In vitro, z.PAAR-1 showed decreased survival rates in Large Yellow Croaker macrophage cell line (LYC-FM) due to impaired oxidative stress tolerance. In vivo, infection with z.PAAR-1 led to a significant reduction in mortality, bacterial colonization, and the formation of spleen nodules in Large Yellow Croakers. Comparative transcriptome analysis revealed that PAAR-1 predominantly influences the host Toll-like receptor (TLR) signaling pathway and apoptosis by upregulating the expression of plasma membrane-associated TLRs, including TLR1, TLR2, and TLR5, while downregulating the expression of endosomal TLRs like TLR3, TLR7, TLR8, and TLR9, along with its downstream molecules such as MyD88 and TRAF3. Additionally, knockout PAAR1 downregulates apoptosis-related genes including AP-1, NF-kappa B, FAS-L TNF alpha, Caspase8, and FAS-L. Real-time quantitative polymerase chain reaction (RT-qPCR) further confirmed these findings. Furthermore, the proportion of apoptotic cells was significantly lower in the z.PAAR-1 infected LYC-PKM cells. These results indicate that PAAR-1 is involved in regulating TLR signaling pathway and apoptosis in Large Yellow Croaker. This study provides the first identification of the core T6SS-1 gene PAAR-1 in P. plecoglossicida, offering valuable insights into its pathogenic mechanisms and presenting a potential target for attenuated vaccine development.
Keyword ：
Immune response Immune response Large yellow croaker Large yellow croaker PAAR-1 PAAR-1 Pathogenicity Pathogenicity Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Zhang, Baoyu , Li, Youshen , Li, Jianxin et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) [J]. | AQUACULTURE , 2026 , 610 . |
| MLA | Zhang, Baoyu et al. "Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea)" . | AQUACULTURE 610 (2026) . |
| APA | Zhang, Baoyu , Li, Youshen , Li, Jianxin , Zhai, Yu , Meng, Ziyu , Huang, Xiyue et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) . | AQUACULTURE , 2026 , 610 . |
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本发明提供了一株乳酸乳球菌R3及其在防治大黄鱼内脏白点病上的应用。所述菌株分类命名为乳酸乳球菌（Lactococcus lactis）R3，已于2024年10月21日保藏于中国典型培养物保藏中心，保藏编号为CCTCC M 20242278。该菌株从健康的大黄鱼肠道中分离和鉴定获得，具有抑菌性、非溶血性，经抑菌试验表明，乳酸乳球菌R3抑菌谱广，生防效果好，其发酵液能有效抑制变形假单胞菌、嗜水气单胞菌、大肠杆菌、副溶血弧菌和溶藻弧菌。将菌体或发酵液作为饲料添加剂能够起到防治内脏白点病的作用，为替代抗生素治疗内脏白点病提供新思路，也为鱼源微生态制剂提供可靠的备用菌种。
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| GB/T 7714 | 陈新华 , 曾建平 , 林智鑫 et al. 一株乳酸乳球菌R3及其在防治大黄鱼内脏白点病中的应用 : CN202510024011.1[P]. | 2025-01-07 . |
| MLA | 陈新华 et al. "一株乳酸乳球菌R3及其在防治大黄鱼内脏白点病中的应用" : CN202510024011.1. | 2025-01-07 . |
| APA | 陈新华 , 曾建平 , 林智鑫 , 敖敬群 . 一株乳酸乳球菌R3及其在防治大黄鱼内脏白点病中的应用 : CN202510024011.1. | 2025-01-07 . |
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本发明属于基因工程领域，尤其涉及一种大黄鱼SOCS1基因启动子及其应用。所述大黄鱼SOCS1基因启动子的核苷酸序列如SEQ ID NO.1所示。将所述大黄鱼SOCS1基因启动子构建至pGL3.0载体，转染至鲤上皮瘤细胞（EPC）具有较高的基础活性，突变干扰素刺激响应元件（ISRE）基序，大黄鱼SOCS1基因启动子的活性显著下降；在病毒类似物poly(I:C)刺激下，该所述大黄鱼SOCS1基因启动子的活性进一步提高，而突变的启动子活性下降。本发明提供的所述大黄鱼SOCS1基因启动子可用于启动目的基因表达、鱼类抗病毒免疫机制研究及鱼类转基因育种。
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| GB/T 7714 | 母尹楠 , 陈新华 , 敖敬群 et al. 一种大黄鱼SOCS1基因启动子及其应用 : CN202510121197.2[P]. | 2025-01-26 . |
| MLA | 母尹楠 et al. "一种大黄鱼SOCS1基因启动子及其应用" : CN202510121197.2. | 2025-01-26 . |
| APA | 母尹楠 , 陈新华 , 敖敬群 , 陈华枝 , 刘佳美 . 一种大黄鱼SOCS1基因启动子及其应用 : CN202510121197.2. | 2025-01-26 . |
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本发明公开了一种调控大黄鱼淋巴细胞增殖与分化的miRNA模拟物及其应用，属于分子生物技术与基因工程领域。所述miRNA为miR‑146a，本发明提供miR‑146a模拟物由正义链和反义链合成，其正义链序列为5'‑UGAGAACUGAAUUCCAUAGAUGG‑3'，反义链序列为5'‑ AUCUAUGGAAUUCAGUUCUCAUU ‑3'；该miR‑146a模拟物可在体内外显著促进大黄鱼T、B淋巴细胞的增殖，并促进大黄鱼T淋巴细胞向Th1、Th2型细胞方向分化，抑制其向Th17型细胞方向分化，从而增强大黄鱼的适应性免疫反应。因此miR‑146a模拟物具有开发成为大黄鱼免疫调节剂的潜在应用价值。
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| GB/T 7714 | 敖敬群 , 陈新华 , 蒋吉敏 et al. 一种调控大黄鱼淋巴细胞增殖与分化的miRNA模拟物及其应用 : CN202510179021.2[P]. | 2025-02-18 . |
| MLA | 敖敬群 et al. "一种调控大黄鱼淋巴细胞增殖与分化的miRNA模拟物及其应用" : CN202510179021.2. | 2025-02-18 . |
| APA | 敖敬群 , 陈新华 , 蒋吉敏 , 张纪元 . 一种调控大黄鱼淋巴细胞增殖与分化的miRNA模拟物及其应用 : CN202510179021.2. | 2025-02-18 . |
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The mitochondrial antiviral signaling protein (MAVS) relays signals from RIG-I-like receptors (RLRs) to induce type I interferon (IFN) production. In teleost fish, MAVS expression is significantly upregulated in response to viral infections or synthetic double-stranded RNA (dsRNA), whereas mammalian MAVS does not exhibit a similar response. However, the mechanisms regulating MAVS expression in teleosts remain unclear. In this study, we demonstrate that the viral mimic poly(I:C)-induced upregulation of Larimichthys crocea (Lc) MAVS occurs via the type I IFN signaling pathway. Inhibition of the JAK-STAT pathway significantly suppressed both poly(I:C)- and LcIFNi-induced LcMAVS expression. Further analysis revealed that an enhancer in the 5 '- untranslated region (UTR) intron of LcMAVS contains two functional interferon-stimulated response elements (ISREs), which are crucial for its activation. The enhancer activity of LcMAVS is regulated by interferon regulatory factors (IRFs), including IRF1, IRF3, IRF7, IRF9, and IRF11. These IRFs form several heterodimeric complexes, such as IRF1/3, IRF1/7, IRF3/7, and IRF3/11, to mediate LcMAVS enhancer activation. Structural analysis indicates that the ISRE motifs in the intronic enhancer can accommodate two or three DNA-binding domains (DBDs) from IRFs. These findings provide a potential explanation for the differential regulation of MAVS in response to stimuli in teleosts and mammals. Furthermore, our study demonstrates that MAVS is an interferon-stimulated gene (ISG) in a marine fish, providing insights into the evolutionary divergence of the vertebrate RLR signaling pathway.
Keyword ：
Interferon Interferon Intronic enhancer Intronic enhancer IRFs IRFs Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) MAVS MAVS
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| GB/T 7714 | Li, Wenxing , Feng, Yuan , Chen, Huazhi et al. Identification of a type I IFN- and IRF-inducible enhancer in the 5′-UTR intron of MAVS in large yellow croaker Larimichthys crocea [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
| MLA | Li, Wenxing et al. "Identification of a type I IFN- and IRF-inducible enhancer in the 5′-UTR intron of MAVS in large yellow croaker Larimichthys crocea" . | FISH & SHELLFISH IMMUNOLOGY 160 (2025) . |
| APA | Li, Wenxing , Feng, Yuan , Chen, Huazhi , Ao, Jingqun , Chen, Xinhua . Identification of a type I IFN- and IRF-inducible enhancer in the 5′-UTR intron of MAVS in large yellow croaker Larimichthys crocea . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
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Melanoma differentiation-associated gene 5 (MDA5) initiates type I interferon (IFN) production by detecting cytosolic viral RNA. Mammalian MDA5 is an IFN-inducible gene and controlled by IFN regulatory factor 1 (IRF1). Teleost MDA5 also induces type I IFN production in response to viruses, yet its regulation remains largely unexplored. This study used the large yellow croaker Larimichthys crocea (Lc) as a model organism and revealed that a type I IFN (LcIFNi) triggers the expression of LcMDA5 through the JAK-STAT signaling pathway, which involves phosphorylation of LcIRF11. LcMDA5 was transcriptionally regulated by LcIRF11. Mechanistically, LcIRF11 interacts with the IFN-stimulated response element within the LcMDA5 promoter, via a3 helix and loop1, and loop2 and loop3 in its DNA binding domain. Overexpression of LcIRF11 recruits p300 and RNA polymerase II (Pol II) to the LcMDA5 promoter region. Pull-down analysis further confirmed the interaction of LcIRF11 with these two proteins. This recruitment was accompanied by increased levels of his- tone H3K27 acetylation (H3K27ac) and histone H3K4 trimethylation (H3K4me3), both of which are strongly associated with active transcription. Conversely, silencing LcIRF11 reduced p300 and Pol II recruitments and hindered the enrichment of H3K27ac/H3K4me3 modifications at the LcMDA5 promoter. Thus, here we present the first report of IRF11 orchestrating the activation of MDA5 transcription by binding to the IFN-stimulated response element of MDA5 promoter and forming a transcriptional complex with p300 and Pol II. Our results revealed an ancient regulatory mechanism of MDA5 in lower vertebrates, providing insights into its function and evolution.
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| GB/T 7714 | Li, Wenxing , Feng, Yuan , Teng, Yan et al. P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11 [J]. | JOURNAL OF BIOLOGICAL CHEMISTRY , 2025 , 301 (2) . |
| MLA | Li, Wenxing et al. "P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11" . | JOURNAL OF BIOLOGICAL CHEMISTRY 301 . 2 (2025) . |
| APA | Li, Wenxing , Feng, Yuan , Teng, Yan , Montero, Alvaro Fernandez , Zhou, Yuanyuan , Zhang, Xiangyang et al. P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11 . | JOURNAL OF BIOLOGICAL CHEMISTRY , 2025 , 301 (2) . |
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Large yellow croaker (Larimichthys crocea) is an economically important fish, with the annual production ranking second among maricultured fish in China. Outbreaks of visceral white nodules disease caused by Pseudomonas plecoglossicida have led to substantial economic losses for the L. crocea aquaculture industry. However, L. crocea defense strategies against P. plecoglossicida infection, especially the role of microRNAs (miRNAs) in the defense against P. plecoglossicida, are poorly understood. Here, we analyzed changes in the mRNA and miRNA expression profiles in the spleen of L. crocea at 96 h post-infection and explored its defensive strategies. Principal component analysis (PCA) showed that P. plecoglossicida infection brought about a profound remodeling of both the miRNA and mRNA profiles. Enrichment analysis showed that the inflammatory response (IL-17 signaling pathway, chemokines and chemokine receptor pathway), ATP synthesis (TCA cycle and oxidative phosphorylation), apoptosis and necroptosis (TNF signaling pathway), and proteolysis (proteasome pathway) were enriched and upregulated by P. plecoglossicida. Thus, P. plecoglossicida infection activated the inflammatory response, stimulated ATP synthesis, and accelerated apoptosis and necroptosis, and promoted proteasome-mediated protein degradation. Additionally, integrated analysis identified 568 miRNA-mRNA pairs. KEGG enrichment analysis of the miRNA targets showed that the enriched pathways included cytokine-cytokine receptor interaction, the chemokine signaling pathway, the C-type lectin receptor signaling pathway, and apoptosis. Integrated analysis identified 14 miRNAs which targeted 44 immune-related genes. Altogether, our results revealed not only the role of the inflammatory response, energy metabolism, apoptosis and necroptosis, and the proteasome pathway in L. crocea defense against P. plecoglossicida infection, but also the regulatory networks of miRNAs associated with host defense against P. plecoglossicida.
Keyword ：
comparative transcriptomics comparative transcriptomics Larimichthys crocea Larimichthys crocea microRNAs microRNAs Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Shao, Guangming , Zhang, Yameng , Zhou, Zhaolong et al. Integrated transcriptome analysis of mRNA and miRNA revealed defensive strategies in the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida infection [J]. | ACTA OCEANOLOGICA SINICA , 2025 , 44 (2) : 37-51 . |
| MLA | Shao, Guangming et al. "Integrated transcriptome analysis of mRNA and miRNA revealed defensive strategies in the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida infection" . | ACTA OCEANOLOGICA SINICA 44 . 2 (2025) : 37-51 . |
| APA | Shao, Guangming , Zhang, Yameng , Zhou, Zhaolong , Zhao, Zexu , He, Fengjiao , Ji, Jiawen et al. Integrated transcriptome analysis of mRNA and miRNA revealed defensive strategies in the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida infection . | ACTA OCEANOLOGICA SINICA , 2025 , 44 (2) , 37-51 . |
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B细胞淋巴瘤-2修饰因子（Bcl-2-modifying factor, Bmf）是Bcl-2家族中仅含BH3结构域促凋亡蛋白亚家族的一员，当凋亡刺激存在时，Bmf在真核生物细胞中启动细胞凋亡，从而在胚胎发育、器官发生、肿瘤抑制中发挥重要作用。目前，有关低等脊椎动物Bmf的研究较少，仅在斑马鱼（Danio rerio）中有相关报道。本研究通过序列比对、基因共线性分析及进化分析证实与高等脊椎动物Bmf主要以单个基因的不同剪切异构体形式存在不同，鱼类具有Bmf1、Bmf2两种不同的基因。从大黄鱼（Larimichthys crocea）中克隆获得了其Bmf2(LcBmf2)的开放阅读框序列，该序列全长561 bp，编码187个氨基酸。尽管Lc Bmf2的氨基酸序列与人和小鼠Bmf的一致性较低，但具有对Bmf功能至关重要的DLC2结合基序和BH3结构域，提示其可能具有与哺乳动物Bmf相似的功能。大黄鱼Bmf2在HEK-293T细胞系中的过表达可诱导HEK-293T细胞脱壁、形态改变以及细胞内凋亡相关Caspase 3、Caspase 8的酶活性升高，展现出较强的促凋亡功能。这是鱼类Bmf在细胞与分子水平促细胞凋亡功能的首次报道，为进一步研究Bmf在鱼类细胞凋亡中的作用及分子机制奠定了基础。
Keyword ：
Bmf Bmf 大黄鱼 大黄鱼 序列分析 序列分析 细胞凋亡 细胞凋亡
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| GB/T 7714 | 蒋吉敏 , 张纪元 , 黎球华 et al. 大黄鱼Bmf2的基因克隆、序列分析及促细胞凋亡功能研究 [J]. | 应用海洋学学报 , 2025 , 44 (02) : 312-319 . |
| MLA | 蒋吉敏 et al. "大黄鱼Bmf2的基因克隆、序列分析及促细胞凋亡功能研究" . | 应用海洋学学报 44 . 02 (2025) : 312-319 . |
| APA | 蒋吉敏 , 张纪元 , 黎球华 , 陈依婷 , 陈新华 , 敖敬群 . 大黄鱼Bmf2的基因克隆、序列分析及促细胞凋亡功能研究 . | 应用海洋学学报 , 2025 , 44 (02) , 312-319 . |
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Chemokines are essential components of the vertebrate immune system, playing a crucial role in modulating the migration and functional activation of leukocytes. Among the chemokine superfamily, CC chemokines constitute the largest subgroup in mammals. Although this subfamily exhibits a significant expansion in teleosts, its functional roles remain largely unexplored. In this study, we first identified a Regakine-1 homologue (LcR1), a CC chemokine family member, in large yellow croaker (Larimichthys crocea). The deduced LcR1 protein consists of 142 amino acids, including a 22-residue signal peptide and a 120-residue mature peptide with a conserved CC motif within its chemokine (SCY) domain. Phylogenetic analysis revealed that LcR1 clusters with Regakine-1 homologues from other species. LcR1 mRNA was constitutively expressed in all examined tissues, albeit at varying levels. Following challenge with Cryptocaryon irritans or Pseudomonas plecoglossicida, its transcripts were significantly upregulated in immune-related tissues (head kidney, spleen, gills, and skin), suggesting its involvement in pathogen defense. Recombinant LcR1 (rLcR1) protein not only chemoattracted granulocytes from primary head kidney leukocytes (PKLs) but also enhanced phagocytic activity and respiratory burst of PKLs. Furthermore, rLcR1 significantly upregulated mRNA levels of pro-inflammatory cytokines (il1 beta, il6, cxcl8_l1, and ifn gamma) in PKLs. Collectively, these findings provide the first evidence that fish Regakine-1 acts as an inflammatory chemokine regulating leukocyte migration and activation.
Keyword ：
CC chemokine CC chemokine Inflammatory response Inflammatory response Innate immunity Innate immunity Leukocyte chemotaxis Leukocyte chemotaxis Phagocytosis Phagocytosis Respiratory burst Respiratory burst Teleost immunity Teleost immunity
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| GB/T 7714 | Wang, Meiyan , Li, Qiuhua , Li, Chenhao et al. Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
| MLA | Wang, Meiyan et al. "Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 166 (2025) . |
| APA | Wang, Meiyan , Li, Qiuhua , Li, Chenhao , Teng, Yan , Ao, Jingqun , Chen, Xinhua . Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
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