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学者姓名：敖敬群
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Abstract ：
Pseudomonas plecoglossicida is a pathogen bacterium responsible for visceral white spot disease (VWND) in Large Yellow Croakers (Larimichthys crocea), causing significant economic losses in commercial fish farms. The prolinealanine-alanine-arginine repeats protein (PAAR) is a core component of the spike structure in the type VI secretion system (T6SS), which injects toxic effectors into host and contributes to bacterial virulence. However, the role of the PAAR gene in P. plecoglossicida and its impact on bacterial infection and host immune responses remain unexplored. In this study, PAAR-1 was identified for the first time in P. plecoglossicida as an effector gene within the T6SS-1 gene cluster, which is regulated and secreted by T6SS-1. The P. plecoglossicida mutant strain (z.PAAR-1) and its complementary strain (C-z.PAAR-1) were constructed for subsequent investigation. Compared to the wild-type strain, z.PAAR-1 exhibited reduced biofilm formation, adhesion, total antioxidant capacity, and secretion of T6SS core protein Hcp-1. In vitro, z.PAAR-1 showed decreased survival rates in Large Yellow Croaker macrophage cell line (LYC-FM) due to impaired oxidative stress tolerance. In vivo, infection with z.PAAR-1 led to a significant reduction in mortality, bacterial colonization, and the formation of spleen nodules in Large Yellow Croakers. Comparative transcriptome analysis revealed that PAAR-1 predominantly influences the host Toll-like receptor (TLR) signaling pathway and apoptosis by upregulating the expression of plasma membrane-associated TLRs, including TLR1, TLR2, and TLR5, while downregulating the expression of endosomal TLRs like TLR3, TLR7, TLR8, and TLR9, along with its downstream molecules such as MyD88 and TRAF3. Additionally, knockout PAAR1 downregulates apoptosis-related genes including AP-1, NF-kappa B, FAS-L TNF alpha, Caspase8, and FAS-L. Real-time quantitative polymerase chain reaction (RT-qPCR) further confirmed these findings. Furthermore, the proportion of apoptotic cells was significantly lower in the z.PAAR-1 infected LYC-PKM cells. These results indicate that PAAR-1 is involved in regulating TLR signaling pathway and apoptosis in Large Yellow Croaker. This study provides the first identification of the core T6SS-1 gene PAAR-1 in P. plecoglossicida, offering valuable insights into its pathogenic mechanisms and presenting a potential target for attenuated vaccine development.
Keyword ：
Immune response Immune response Large yellow croaker Large yellow croaker PAAR-1 PAAR-1 Pathogenicity Pathogenicity Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Zhang, Baoyu , Li, Youshen , Li, Jianxin et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) [J]. | AQUACULTURE , 2026 , 610 . |
| MLA | Zhang, Baoyu et al. "Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea)" . | AQUACULTURE 610 (2026) . |
| APA | Zhang, Baoyu , Li, Youshen , Li, Jianxin , Zhai, Yu , Meng, Ziyu , Huang, Xiyue et al. Identification and characterization of the PAAR-1 gene in Pseudomonas plecoglossicida: Insights into bacterial phenotypes and host immune responses in Large Yellow Croaker (Larimichthys crocea) . | AQUACULTURE , 2026 , 610 . |
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Signal transducer and activator of transcription (STAT) family members, which serve as transcription activators, play a central role in regulating inflammatory responses. While STATs have been well-characterized in mammals, systematic studies in fish remain scarce. In the present study, eight STATs in Cromileptes altivelis-CaSTAT1a, CaSTAT1b, CaSTAT2, CaSTAT3, CaSTAT4, CaSTAT5a, CaSTAT5b, and CaSTAT6-were characterized using PCR cloning and bioinformatics approaches. Phylogenetic analysis indicated that STATs have undergone significant evolutionary conservation throughout their lineage. Tissue expression patterns in healthy fish revealed that the eight CaSTATs displayed spatiotemporal specificity, with greater expression observed in gill, blood, and muscle. In response to bacterial (Vibrio harveyi, Streptococcus agalactiae) or viral (nervous necrosis virus, NNV) challenge, CaSTATs were robustly expressed and exhibited pathogen-, tissue-, and time-dependent expression divergence. In summary, our data highlight the indispensable roles of eight CaSTATs in modulating host immune responses.
Keyword ：
Cromileptes altivelis Cromileptes altivelis Expression patterns Expression patterns Immune defense Immune defense Pathogens challenge Pathogens challenge STAT STAT
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| GB/T 7714 | Zhang, Panpan , Lin, Shuyi , Xie, Zixin et al. Pathogen-driven modulation of eight STATs in Cromileptes altivelis: Comparative analysis from homeostasis to immune activation [J]. | FISH & SHELLFISH IMMUNOLOGY , 2026 , 168 . |
| MLA | Zhang, Panpan et al. "Pathogen-driven modulation of eight STATs in Cromileptes altivelis: Comparative analysis from homeostasis to immune activation" . | FISH & SHELLFISH IMMUNOLOGY 168 (2026) . |
| APA | Zhang, Panpan , Lin, Shuyi , Xie, Zixin , Chen, Guisen , Cao, Zhenjie , Zhang, Chen et al. Pathogen-driven modulation of eight STATs in Cromileptes altivelis: Comparative analysis from homeostasis to immune activation . | FISH & SHELLFISH IMMUNOLOGY , 2026 , 168 . |
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Dietary Astragalus polysaccharides (APS) get wide application in aquaculture due to their excellent immunoregulatory effects. However, little is known about the effects of dietary APS on vaccine potency in fish. In the present study, large yellow croakers (Larimichthys crocea) were injected with formalin-inactivated Pseudomonas plecoglossicida after APS feeding for 14 d and then challenged by live P. plecoglossicida on 28 d post-vaccination. The results showed that dietary APS combined with inactivated vaccine could improve the survival rate, and alleviate splenic lesions and bacteria load post-challenge, thus exhibiting a better protection in large yellow croaker against P. plecoglossicida infection than inactivated vaccine treatment alone. Fish in APS + P. plecoglossicida vaccine group expressed a better antioxidant status by possessing a relatively higher serum total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity and a significantly lower malondialdehyde (MDA) content than those in vaccine alone group. Serum lysozyme (LZM) and alkaline phosphatase (AKP) activities, and immunoglobulin M (IgM) titers were all improved in fish of APS + P. plecoglossicida vaccine group compared to fish in vaccine group. Furthermore, fish in APS + P. plecoglossicida vaccine group showed a lower down-regulation of pro-inflammatory cytokines interleukin-1 beta (IL-1 beta) and IL-6, and a higher up-regulation of antiinflammatory cytokine IL-10, immunoglobulin (IgM) and T cell immunity-related cytokines, interferon-gamma (IFN-gamma), IL-4/13A, and IL-4/13B, when compared with those in fish of vaccine group. These results suggested that dietary APS could assist inactivated vaccine to trigger stronger innate and adaptive immune responses against P. plecoglossicida infection. These findings further uncover the immunoregulatory mechanism of dietary APS, and provide valuable information for prevention and control of bacteriosis in fish.
Keyword ：
Astragalus polysaccharides Astragalus polysaccharides Immune response Immune response Inactivated vaccine Inactivated vaccine Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea ) Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Song, Yueyang , Chen, Hui , An, Huimin et al. Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker ( Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 157 . |
| MLA | Song, Yueyang et al. "Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker ( Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 157 (2025) . |
| APA | Song, Yueyang , Chen, Hui , An, Huimin , Wang, Yongyang , Shao, Jianchun , Yan, Meijiao et al. Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker ( Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 157 . |
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Melanoma differentiation-associated gene 5 (MDA5) initiates type I interferon (IFN) production by detecting cytosolic viral RNA. Mammalian MDA5 is an IFN-inducible gene and controlled by IFN regulatory factor 1 (IRF1). Teleost MDA5 also induces type I IFN production in response to viruses, yet its regulation remains largely unexplored. This study used the large yellow croaker Larimichthys crocea (Lc) as a model organism and revealed that a type I IFN (LcIFNi) triggers the expression of LcMDA5 through the JAK-STAT signaling pathway, which involves phosphorylation of LcIRF11. LcMDA5 was transcriptionally regulated by LcIRF11. Mechanistically, LcIRF11 interacts with the IFN-stimulated response element within the LcMDA5 promoter, via a3 helix and loop1, and loop2 and loop3 in its DNA binding domain. Overexpression of LcIRF11 recruits p300 and RNA polymerase II (Pol II) to the LcMDA5 promoter region. Pull-down analysis further confirmed the interaction of LcIRF11 with these two proteins. This recruitment was accompanied by increased levels of his- tone H3K27 acetylation (H3K27ac) and histone H3K4 trimethylation (H3K4me3), both of which are strongly associated with active transcription. Conversely, silencing LcIRF11 reduced p300 and Pol II recruitments and hindered the enrichment of H3K27ac/H3K4me3 modifications at the LcMDA5 promoter. Thus, here we present the first report of IRF11 orchestrating the activation of MDA5 transcription by binding to the IFN-stimulated response element of MDA5 promoter and forming a transcriptional complex with p300 and Pol II. Our results revealed an ancient regulatory mechanism of MDA5 in lower vertebrates, providing insights into its function and evolution.
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| GB/T 7714 | Li, Wenxing , Feng, Yuan , Teng, Yan et al. P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11 [J]. | JOURNAL OF BIOLOGICAL CHEMISTRY , 2025 , 301 (2) . |
| MLA | Li, Wenxing et al. "P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11" . | JOURNAL OF BIOLOGICAL CHEMISTRY 301 . 2 (2025) . |
| APA | Li, Wenxing , Feng, Yuan , Teng, Yan , Montero, Alvaro Fernandez , Zhou, Yuanyuan , Zhang, Xiangyang et al. P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11 . | JOURNAL OF BIOLOGICAL CHEMISTRY , 2025 , 301 (2) . |
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The mitochondrial antiviral signaling protein (MAVS) relays signals from RIG-I-like receptors (RLRs) to induce type I interferon (IFN) production. In teleost fish, MAVS expression is significantly upregulated in response to viral infections or synthetic double-stranded RNA (dsRNA), whereas mammalian MAVS does not exhibit a similar response. However, the mechanisms regulating MAVS expression in teleosts remain unclear. In this study, we demonstrate that the viral mimic poly(I:C)-induced upregulation of Larimichthys crocea (Lc) MAVS occurs via the type I IFN signaling pathway. Inhibition of the JAK-STAT pathway significantly suppressed both poly(I:C)- and LcIFNi-induced LcMAVS expression. Further analysis revealed that an enhancer in the 5 '- untranslated region (UTR) intron of LcMAVS contains two functional interferon-stimulated response elements (ISREs), which are crucial for its activation. The enhancer activity of LcMAVS is regulated by interferon regulatory factors (IRFs), including IRF1, IRF3, IRF7, IRF9, and IRF11. These IRFs form several heterodimeric complexes, such as IRF1/3, IRF1/7, IRF3/7, and IRF3/11, to mediate LcMAVS enhancer activation. Structural analysis indicates that the ISRE motifs in the intronic enhancer can accommodate two or three DNA-binding domains (DBDs) from IRFs. These findings provide a potential explanation for the differential regulation of MAVS in response to stimuli in teleosts and mammals. Furthermore, our study demonstrates that MAVS is an interferon-stimulated gene (ISG) in a marine fish, providing insights into the evolutionary divergence of the vertebrate RLR signaling pathway.
Keyword ：
Interferon Interferon Intronic enhancer Intronic enhancer IRFs IRFs Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) MAVS MAVS
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| GB/T 7714 | Li, Wenxing , Feng, Yuan , Chen, Huazhi et al. Identification of a type I IFN- and IRF-inducible enhancer in the 5′-UTR intron of MAVS in large yellow croaker Larimichthys crocea [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
| MLA | Li, Wenxing et al. "Identification of a type I IFN- and IRF-inducible enhancer in the 5′-UTR intron of MAVS in large yellow croaker Larimichthys crocea" . | FISH & SHELLFISH IMMUNOLOGY 160 (2025) . |
| APA | Li, Wenxing , Feng, Yuan , Chen, Huazhi , Ao, Jingqun , Chen, Xinhua . Identification of a type I IFN- and IRF-inducible enhancer in the 5′-UTR intron of MAVS in large yellow croaker Larimichthys crocea . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
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本发明属于基因工程领域，尤其涉及一种大黄鱼SOCS1基因启动子及其应用。所述大黄鱼SOCS1基因启动子的核苷酸序列如SEQ ID NO.1所示。将所述大黄鱼SOCS1基因启动子构建至pGL3.0载体，转染至鲤上皮瘤细胞（EPC）具有较高的基础活性，突变干扰素刺激响应元件（ISRE）基序，大黄鱼SOCS1基因启动子的活性显著下降；在病毒类似物poly(I:C)刺激下，该所述大黄鱼SOCS1基因启动子的活性进一步提高，而突变的启动子活性下降。本发明提供的所述大黄鱼SOCS1基因启动子可用于启动目的基因表达、鱼类抗病毒免疫机制研究及鱼类转基因育种。
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| GB/T 7714 | 母尹楠 , 陈新华 , 敖敬群 et al. 一种大黄鱼SOCS1基因启动子及其应用 : CN202510121197.2[P]. | 2025-01-26 . |
| MLA | 母尹楠 et al. "一种大黄鱼SOCS1基因启动子及其应用" : CN202510121197.2. | 2025-01-26 . |
| APA | 母尹楠 , 陈新华 , 敖敬群 , 陈华枝 , 刘佳美 . 一种大黄鱼SOCS1基因启动子及其应用 : CN202510121197.2. | 2025-01-26 . |
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Large yellow croaker (Larimichthys crocea) is an economically important fish, with the annual production ranking second among maricultured fish in China. Outbreaks of visceral white nodules disease caused by Pseudomonas plecoglossicida have led to substantial economic losses for the L. crocea aquaculture industry. However, L. crocea defense strategies against P. plecoglossicida infection, especially the role of microRNAs (miRNAs) in the defense against P. plecoglossicida, are poorly understood. Here, we analyzed changes in the mRNA and miRNA expression profiles in the spleen of L. crocea at 96 h post-infection and explored its defensive strategies. Principal component analysis (PCA) showed that P. plecoglossicida infection brought about a profound remodeling of both the miRNA and mRNA profiles. Enrichment analysis showed that the inflammatory response (IL-17 signaling pathway, chemokines and chemokine receptor pathway), ATP synthesis (TCA cycle and oxidative phosphorylation), apoptosis and necroptosis (TNF signaling pathway), and proteolysis (proteasome pathway) were enriched and upregulated by P. plecoglossicida. Thus, P. plecoglossicida infection activated the inflammatory response, stimulated ATP synthesis, and accelerated apoptosis and necroptosis, and promoted proteasome-mediated protein degradation. Additionally, integrated analysis identified 568 miRNA-mRNA pairs. KEGG enrichment analysis of the miRNA targets showed that the enriched pathways included cytokine-cytokine receptor interaction, the chemokine signaling pathway, the C-type lectin receptor signaling pathway, and apoptosis. Integrated analysis identified 14 miRNAs which targeted 44 immune-related genes. Altogether, our results revealed not only the role of the inflammatory response, energy metabolism, apoptosis and necroptosis, and the proteasome pathway in L. crocea defense against P. plecoglossicida infection, but also the regulatory networks of miRNAs associated with host defense against P. plecoglossicida.
Keyword ：
comparative transcriptomics comparative transcriptomics Larimichthys crocea Larimichthys crocea microRNAs microRNAs Pseudomonas plecoglossicida Pseudomonas plecoglossicida
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| GB/T 7714 | Shao, Guangming , Zhang, Yameng , Zhou, Zhaolong et al. Integrated transcriptome analysis of mRNA and miRNA revealed defensive strategies in the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida infection [J]. | ACTA OCEANOLOGICA SINICA , 2025 , 44 (2) : 37-51 . |
| MLA | Shao, Guangming et al. "Integrated transcriptome analysis of mRNA and miRNA revealed defensive strategies in the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida infection" . | ACTA OCEANOLOGICA SINICA 44 . 2 (2025) : 37-51 . |
| APA | Shao, Guangming , Zhang, Yameng , Zhou, Zhaolong , Zhao, Zexu , He, Fengjiao , Ji, Jiawen et al. Integrated transcriptome analysis of mRNA and miRNA revealed defensive strategies in the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida infection . | ACTA OCEANOLOGICA SINICA , 2025 , 44 (2) , 37-51 . |
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The transcription factor Tcf3 is a key regulator during mammalian B cell development and activation, which is highly expressed in precursor B cells, downregulated in mature B cells, and re-expressed upon B cell activation. Despite the extensive studies in mammals, its role in teleost B cells remains poorly characterized. Here, using the economically important marine teleost large yellow croaker (Larimichthys crocea) as a model, we identified two Tcf3 homologs (LcTcf3a and LcTcf3b) and found that both genes were upregulated during B cell activation. By cloning the predicted promoter region and serial truncation, we found that the core promoter regions of LcTcf3a and LcTcf3b were located about 600 bp around the transcription start sites. Interestingly, while both of the two gene promoters contain CpG islands, bisulfite sequencing showed constitutive hypomethylation both before and after B cell activation. However, in vitro methylation suppressed the promoter activities of LcTcf3a and LcTcf3b, indicating their DNA methylation-dependent regulation. Together, these results suggested a "poised" state of LcTcf3a and LcTcf3b in resting B cells, where LcTcf3a and LcTcf3b were repressed but preparing for fast upregulation during immune response. Our findings proposed that LcTcf3a and LcTcf3b likely contribute to B cell activation in a teleost model and their promoter activities may be regulated by DNA methylation, thus providing insights into the understanding of the teleost B cell immunity.
Keyword ：
DNA methylation DNA methylation IgM plus B cells IgM plus B cells Large yellow croaker (Larimichthys crocea) Large yellow croaker (Larimichthys crocea) Tcf3 Tcf3
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| GB/T 7714 | Chen, Qiuxuan , Zhu, Zhuo , Li, Xinran et al. Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM plus B cell activation in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
| MLA | Chen, Qiuxuan et al. "Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM plus B cell activation in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 166 (2025) . |
| APA | Chen, Qiuxuan , Zhu, Zhuo , Li, Xinran , Lai, Guolong , Cui, Zhengwei , Chen, Xinhua et al. Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM plus B cell activation in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 166 . |
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IL-4 and IL-13 are cytokines with structural homology, exerting critical functions in the Th2 immune response via binding to their respective receptor complexes and activating the MAPK and mTOR pathways. MicroRNAs (miRNAs) modulate diverse molecular processes via suppressing messenger RNA translation or inducing messenger RNA degradation. Although miR-7565 has been previously linked to embryonic development, its regulatory effects on the Th2 immune response remain unknown. In this study, miR-7565 was first identified in large yellow croaker (Larimichthys crocea), which exhibits high conservation across vertebrates. The expression of Lcil-13r alpha 1 is negatively regulated by miR-7565 through its direct binding to the 3 ' UTR of Lcil-13r alpha 1. MiR-7565 overexpression led to a notable decrease in endogenous Lcil-13r alpha 1 mRNA levels. MiR-7565 overexpression notably suppressed the activation of MEK1/2 and 4E-BP mediated by LcIL-4/13A and LcIL-4/13B. Moreover, miR-7565 weakened the stimulating impacts of LcIL-4/13A and LcIL-4/13B on T cell proliferation/differentiation, and B cell proliferation. These findings indicate that miR-7565 suppresses the signal transduction induced by IL-4/13A and IL-4/13B by targeting Lcil-13r alpha 1, thereby negatively regulating the Th2 immune response. This study advances our knowledge of miRNA-mediated regulatory mechanisms of adaptive immunity in fish.
Keyword ：
IL-13R alpha 1 IL-13R alpha 1 Large yellow croaker ( Larimichthys crocea ) Large yellow croaker ( Larimichthys crocea ) miR-7565 miR-7565 Th2 immune response Th2 immune response
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| GB/T 7714 | Chen, Huazhi , Liu, Jiamei , Li, Wenxing et al. MicroRNA-7565 negatively regulates the IL-4/13-mediated Th2 immune response by targeting IL-13Rα1 in large yellow croaker (Larimichthys crocea) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 167 . |
| MLA | Chen, Huazhi et al. "MicroRNA-7565 negatively regulates the IL-4/13-mediated Th2 immune response by targeting IL-13Rα1 in large yellow croaker (Larimichthys crocea)" . | FISH & SHELLFISH IMMUNOLOGY 167 (2025) . |
| APA | Chen, Huazhi , Liu, Jiamei , Li, Wenxing , Ao, Jinqun , Chen, Xinhua , Mu, Yinnan . MicroRNA-7565 negatively regulates the IL-4/13-mediated Th2 immune response by targeting IL-13Rα1 in large yellow croaker (Larimichthys crocea) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 167 . |
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B细胞淋巴瘤-2修饰因子（Bcl-2-modifying factor, Bmf）是Bcl-2家族中仅含BH3结构域促凋亡蛋白亚家族的一员，当凋亡刺激存在时，Bmf在真核生物细胞中启动细胞凋亡，从而在胚胎发育、器官发生、肿瘤抑制中发挥重要作用。目前，有关低等脊椎动物Bmf的研究较少，仅在斑马鱼（Danio rerio）中有相关报道。本研究通过序列比对、基因共线性分析及进化分析证实与高等脊椎动物Bmf主要以单个基因的不同剪切异构体形式存在不同，鱼类具有Bmf1、Bmf2两种不同的基因。从大黄鱼（Larimichthys crocea）中克隆获得了其Bmf2(LcBmf2)的开放阅读框序列，该序列全长561 bp，编码187个氨基酸。尽管Lc Bmf2的氨基酸序列与人和小鼠Bmf的一致性较低，但具有对Bmf功能至关重要的DLC2结合基序和BH3结构域，提示其可能具有与哺乳动物Bmf相似的功能。大黄鱼Bmf2在HEK-293T细胞系中的过表达可诱导HEK-293T细胞脱壁、形态改变以及细胞内凋亡相关Caspase 3、Caspase 8的酶活性升高，展现出较强的促凋亡功能。这是鱼类Bmf在细胞与分子水平促细胞凋亡功能的首次报道，为进一步研究Bmf在鱼类细胞凋亡中的作用及分子机制奠定了基础。
Keyword ：
Bmf Bmf 大黄鱼 大黄鱼 序列分析 序列分析 细胞凋亡 细胞凋亡
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| GB/T 7714 | 蒋吉敏 , 张纪元 , 黎球华 et al. 大黄鱼Bmf2的基因克隆、序列分析及促细胞凋亡功能研究 [J]. | 应用海洋学学报 , 2025 , 44 (02) : 312-319 . |
| MLA | 蒋吉敏 et al. "大黄鱼Bmf2的基因克隆、序列分析及促细胞凋亡功能研究" . | 应用海洋学学报 44 . 02 (2025) : 312-319 . |
| APA | 蒋吉敏 , 张纪元 , 黎球华 , 陈依婷 , 陈新华 , 敖敬群 . 大黄鱼Bmf2的基因克隆、序列分析及促细胞凋亡功能研究 . | 应用海洋学学报 , 2025 , 44 (02) , 312-319 . |
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