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学者姓名：李林明
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Abstract ：
在海洋强国的背景下，各大高校相继开设海洋科学相关的专业。福建农林大学于2021年11月整合涉海领域的优势资源和团队，成立了海洋学院。学院根据相关专业国家标准，结合学校办学条件建立了海洋学院教学实验中心。实验中心的建成，为海洋、水产相关专业实践教学提供了必要条件，为学生科研活动、创新创业提供了重要的物质保障。本文介绍了海洋学院实验室建设背景、建设内容、实验室运行成效等方面的相关内容，旨在为更多海洋、水产类学科实验室、实践平台的规划与建设提供参考资料。
Keyword ：
实践教学 实践教学 实验室建设 实验室建设 水产养殖 水产养殖 海洋科学 海洋科学
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| GB/T 7714 | 李林明 , 何亮华 , 赵超 . 海洋、水产类教学实验室建设——以福建农林大学为例 [J]. | 河北渔业 , 2025 , 3 (04) : 38-40 . |
| MLA | 李林明 等. "海洋、水产类教学实验室建设——以福建农林大学为例" . | 河北渔业 3 . 04 (2025) : 38-40 . |
| APA | 李林明 , 何亮华 , 赵超 . 海洋、水产类教学实验室建设——以福建农林大学为例 . | 河北渔业 , 2025 , 3 (04) , 38-40 . |
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【目的】探究热休克蛋白基因在氧化应激和姜黄素作用下的表达差异，了解姜黄素药效的发挥与热休克蛋白基因表达变化的关系，进一步探讨热休克蛋白对氧化应激条件下鱼类肝细胞中的功能，为鱼类氧化应激的毒理学机制及姜黄素的药理学机制提供理论资料。【方法】对24 h姜黄素预处理（保护组）与无姜黄素预处理（对照组）的罗非鱼肝细胞分别进行0、1、2.5 h的H_2O_2应激，并对肝细胞5种高分子热休克蛋白基因（HSP70、HSP90α、HSP90β、HSP60、HSP75）和5种小分子热休克蛋白基因（HSP30、HSPB1、 HSPB7、HSPB8、HSPB11）的表达水平进行qPCR检测。【结果】与不经H_2O_2处理（0 h）相比，H_2O_2作用1 h能显著提升HSPB1表达量，降低HSP30表达量；H_2O_2作用2.5 h能显著提升HSP90a、 HSPB1的表达量。姜黄素预处理24 h能显著提升HSP70、 HSP90α、HSP30、HSPB1的表达量；姜黄素预处理24 h后再进行H_2O_2应激1 h时，HSP70、HSP90α、HSP30的表达量显著高于H_2O_2单独应激（对照组）；姜黄素预处理24 h后再进行H_2O_2应激2.5 h时，HSP90α、HSP30、HSPB1的表达量显著高于H_2O_2单独应激。其余热休克蛋白在试验过程中无显著差异。【结论】H_2O_2单独应激可引起罗非鱼肝细胞HSP90a、HSP30、HSPB1基因的表达量变化，而姜黄素预处理能提高多种热休克蛋白基因的表达，提高了罗非鱼肝细胞的抗氧化能力，最终抵御H_2O_2引起的氧化应激并维持细胞活力。
Keyword ：
姜黄素 姜黄素 氧化应激 氧化应激 热休克蛋白 热休克蛋白 罗非鱼 罗非鱼
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| GB/T 7714 | 李林明 , 张子平 , 王若璇 et al. 姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响 [J]. | 福建农业学报 , 2023 , 38 (09) : 1030-1036 . |
| MLA | 李林明 et al. "姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响" . | 福建农业学报 38 . 09 (2023) : 1030-1036 . |
| APA | 李林明 , 张子平 , 王若璇 , 黄一帆 . 姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响 . | 福建农业学报 , 2023 , 38 (09) , 1030-1036 . |
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Copper is a common component of industrial heavy metal waste and a major component of some fish medicines, which can cause oxidative stress and damage the health of farmed fish. The Nrf2 signaling pathway is an important pathway related to the oxidative stress on vertebrates. Exploring the effect of copper on the Nrf2 signaling pathway in fish hepatocytes would help improve the understanding of the molecular mechanism of antioxidants in fish hepatocytes and provide theoretical data for relevant toxicological research. Adult tilapia were cultured under properly controlled conditions for two weeks to adapt to laboratory culture conditions. Primary tilapia hepatocytes were obtained by cell culture. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was used to detect the effect of copper ions on the viability of tilapia hepatocytes. The lipid peroxidation level (MDA) and antioxidant ability of tilapia hepatocytes (SOD and CAT activity) were detected. Quantitative PCR (qPCR) was used to detect the differential expression of each gene (Nrf2, Keap1a, Keap1b, CuZnSOD, MnSOD, HO-1, and GSTA) in the Nrf2 signaling pathway. The results suggested that after treatment with 100 & mu;M copper ions for 4 h, 8 h, and 24 h, the viability of hepatocytes significantly decreased (p < 0.05). LDH and MDA after 8 h and 24 h treatment were significantly higher than those in the control group (p < 0.05). CAT activity significantly decreased after 4 h (p < 0.05), and SOD activity significantly decreased after 8 h and 24 h (p < 0.05). The results of qPCR showed that the expression of MnSOD significantly increased after a treatment with copper ions for 4 h, and the expression of Nrf2, Keap1a, CuZnSOD, HO-1 as well as GSTA significantly increased after a treatment with copper ions for 8 h, compared with the control group (p < 0.05). After being treated with copper ions for 24 h, the expression of Nrf2 and CuZnSOD significantly increased compared with the control group (p < 0.05). There was no significant difference in the expression of Keap1b or CAT at each time point. In conclusion, with copper ions exposure, the viability of tilapia hepatocytes was reduced, causing lipid peroxidation, a reduction in the antioxidant capacity of cells, the activation of the Nrf2 signaling pathway, and the increase in the expression of most genes in this pathway, which are defensive responses of hepatocytes to oxidative stress caused by copper ions. This study can provide theoretical data for related toxicological research.
Keyword ：
copper copper Nrf2 Nrf2 Oreochromis niloticus Oreochromis niloticus oxidative stress oxidative stress
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| GB/T 7714 | Li, Linming , Wang, Ruoxuan , Zhang, Ziping . The Effect of Cu2+ Exposure on the Nrf2 Signaling Pathway of Tilapia Hepatocyte, Base on Experiments In Vitro [J]. | FISHES , 2023 , 8 (6) . |
| MLA | Li, Linming et al. "The Effect of Cu2+ Exposure on the Nrf2 Signaling Pathway of Tilapia Hepatocyte, Base on Experiments In Vitro" . | FISHES 8 . 6 (2023) . |
| APA | Li, Linming , Wang, Ruoxuan , Zhang, Ziping . The Effect of Cu2+ Exposure on the Nrf2 Signaling Pathway of Tilapia Hepatocyte, Base on Experiments In Vitro . | FISHES , 2023 , 8 (6) . |
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Curcumin is a polyphenol with antioxidant activity that has been used to protect the health of fish livers. Our previous studies about comparative transcriptome have shown that curcumin can enhance the Nrf2-Keap1 signaling pathway and induce downstream anti-stress genes to maintain cell viability. However, the possible role of miRNAs in the protective mechanism of curcumin is not understood. In this study, the tilapia hepatocyte H2O2 stress model was used, and the miRNA expression profile for four groups (control group, curcumin group, H2O2 group, and protection group) were established by high-throughput sequencing. In our results, 278-333 types of Oreochromis niloticus miRNAs, 309-543 types of conserved miRNAs, and 535-746 types of novel miRNAs were identified in different samples. Differentially expressed miRNAs were identified by comparing miRNA expression profiles among the four groups. The expression levels were confirmed by q-PCR. The target genes of these differentially expressed miRNAs were predicted, and their functional annotations were enriched by GO and KEGG analysis, which revealed that many target genes were involved in "response to stimulus" and "antioxidant activity" in each pair of groups. Several miRNAs related to oxidative stress showed differential expression. For example, in the H2O2 group, the expression of miR-122 was decreased, and the expression of miR-21 and miR489 increased significantly. In the curcumin group, the expression of miR-153b was decreased, and the expression of miR-200a and miR-29 was increased significantly. miR-153b, miR-200a, and miR-29 may be involved in the regulation of the Nrf2-Keap1 signaling pathway by curcumin. This work might provide insights into the molecular mechanisms of miRNA regulation of curcumin on the prevention and alleviation of liver diseases in fish.
Keyword ：
Curcumin Curcumin Hepatocyte Hepatocyte miRNA miRNA Nrf2 Nrf2 Oreochromis niloticus Oreochromis niloticus
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| GB/T 7714 | Li, Linming , Huang, Yifan , Zhang, Ziping . Expression profile of miRNAs involved in the hepatoprotective effects of curcumin against oxidative stress in Nile tilapia [J]. | AQUATIC TOXICOLOGY , 2021 , 237 . |
| MLA | Li, Linming et al. "Expression profile of miRNAs involved in the hepatoprotective effects of curcumin against oxidative stress in Nile tilapia" . | AQUATIC TOXICOLOGY 237 (2021) . |
| APA | Li, Linming , Huang, Yifan , Zhang, Ziping . Expression profile of miRNAs involved in the hepatoprotective effects of curcumin against oxidative stress in Nile tilapia . | AQUATIC TOXICOLOGY , 2021 , 237 . |
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Summer outbreaks of the hepatobiliary syndrome in fish impose a heavy burden on aquaculture in China. Curcumin is a polyphenol with antioxidant activity that has been used to protect the health of fish livers, but the mechanism underlying its protective effect is unclear. In this study, an in vitro model of hepatocyte oxidative damage in Oreochromis niloticus was established using H2O2. Treatment with 5 mM H2O2 for 2.5 h markedly reduced cell viability and antioxidant activity and elevated lactate dehydrogenase (LDH) activity, indicating conditions that can be used to establish an oxidative stress model. Under H2O2 stress, curcumin pretreatment significantly maintained cell viability, reduced malondialdehyde (MDA) levels, and increased superoxide dismutase (SOD) activity. RNA-seq results showed that acute H2O2 treatment resulted in minor changes in gene expression, whereas curcumin changed the expression profile and affected cytochrome P450 (Cyp 450), glutathione (GSH) metabolism, and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Several critical antioxidant defense signaling pathways were identified, and altered expression was confirmed by q-PCR. These results indicate that curcumin might upregulate PPAR expression by increasing Cyp2J2 expression. Further experiments showed that curcumin can upregulate the Nrf2-Keap1 signaling pathway at the transcriptional level, and this upregulation can induce downstream defense genes, including glutamate cysteine ligase catalytic subunit(GCLC) and glutamate cysteine ligase modifier subunit (GCLM), and thereby promote GSH synthesis and the expression of related antioxidases. This study might shed light on the effects of curcumin on the prevention and alleviation of liver diseases in fish.
Keyword ：
Curcumin Curcumin Hepatocyte Hepatocyte Nrf2 Nrf2 Oreochromis niloticus Oreochromis niloticus RNA-seq RNA-seq
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| GB/T 7714 | Li, Linming , Zhang, Ziping , Huang, Yifan . Integrative transcriptome analysis and discovery of signaling pathways involved in the protective effects of curcumin against oxidative stress in tilapia hepatocytes [J]. | AQUATIC TOXICOLOGY , 2020 , 224 . |
| MLA | Li, Linming et al. "Integrative transcriptome analysis and discovery of signaling pathways involved in the protective effects of curcumin against oxidative stress in tilapia hepatocytes" . | AQUATIC TOXICOLOGY 224 (2020) . |
| APA | Li, Linming , Zhang, Ziping , Huang, Yifan . Integrative transcriptome analysis and discovery of signaling pathways involved in the protective effects of curcumin against oxidative stress in tilapia hepatocytes . | AQUATIC TOXICOLOGY , 2020 , 224 . |
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应用组织切片和免疫组织化学的方法在中国鲎脑神经中定位了3种离子通道的分布。研究检测到Na~+通道和K~+通道免疫阳性细胞和阳性纤维,但没有检测到Ca~(2+)通道的免疫阳性。Na~+通道主要分布在中间体神经纤维网和背面中央细胞群,K~+通道分布于背面侧后方第二细胞群和背面中央细胞群。
Keyword ：
中国鲎(Tachypleus tridentatus) 中国鲎(Tachypleus tridentatus) 免疫组化 免疫组化 离子通道 离子通道
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| GB/T 7714 | 李林明 . 三种离子通道在中国鲎脑神经节的分布 [J]. | 河北渔业 , 2020 , (01) : 1-3,53 . |
| MLA | 李林明 . "三种离子通道在中国鲎脑神经节的分布" . | 河北渔业 01 (2020) : 1-3,53 . |
| APA | 李林明 . 三种离子通道在中国鲎脑神经节的分布 . | 河北渔业 , 2020 , (01) , 1-3,53 . |
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本发明公开了一种拟穴青蟹工厂化循环水立体养殖的配合饲料，属于水产养殖技术领域。本发明的拟穴青蟹饲料，包含以下重量份原料：鱼粉250—350份、豆粕150—250份、虾壳粉50—100份、玉米淀粉200—300份、鱿鱼粉20—30份、鱼油20—30份、胆碱5—10份、卵磷脂10—20份、胆固醇5—10份、羧甲基纤维素钠5—10份、海藻酸钠10—20份、多维多矿预混合物10—20份、磷酸二氢钙5—10份。本发明的饲料配方科学合理、制备方法简便快捷，加工成本低，性能好、诱食性强，能够有效地促进拟穴青蟹生长和蜕壳，提高成活率，减轻养殖排泄物对环境的污染。
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| GB/T 7714 | 黄莹 , 黄贞胜 , 张子平 et al. 拟穴青蟹工厂化循环水立体养殖的配合饲料 : CN201910562768.0[P]. | 2019-06-26 . |
| MLA | 黄莹 et al. "拟穴青蟹工厂化循环水立体养殖的配合饲料" : CN201910562768.0. | 2019-06-26 . |
| APA | 黄莹 , 黄贞胜 , 张子平 , 何亮华 , 张秀成 , 林旋 et al. 拟穴青蟹工厂化循环水立体养殖的配合饲料 : CN201910562768.0. | 2019-06-26 . |
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在不同水温、盐度条件下检测了不同规格鱼尾楔蚌的耗氧率,研究发现当水温在20-25℃范围内,耗氧率随着水温的升高而升高,超过25℃,耗氧率则随之下降;在盐度(0~32‰)范围内,耗氧率随着盐度的升高而下降。本文从耗氧率的角度探讨温度和盐度对鱼尾楔蚌呼吸功能的影响。
Keyword ：
水温 水温 盐度 盐度 耗氧率 耗氧率 鱼尾楔蚌 鱼尾楔蚌
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| GB/T 7714 | 李林明 , 何亮华 . 水温、盐度对不同规格鱼尾楔蚌耗氧率的影响 [J]. | 河北渔业 , 2016 , (02) : 13-15 . |
| MLA | 李林明 et al. "水温、盐度对不同规格鱼尾楔蚌耗氧率的影响" . | 河北渔业 02 (2016) : 13-15 . |
| APA | 李林明 , 何亮华 . 水温、盐度对不同规格鱼尾楔蚌耗氧率的影响 . | 河北渔业 , 2016 , (02) , 13-15 . |
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以淀粉酶活力、纤维素酶活力和总抗氧化能力为指标,研究饥饿与再投喂期间菲律宾蛤仔消化能力和抗氧化能力的变化,结果表明:饥饿与再投喂对菲律宾蛤仔抗氧化能力无显著影响,但可提高其消化能力;蛤仔淀粉酶、纤维素酶活力呈现随饥饿时间延长而下降的趋势,饥饿阶段各组消化酶活力在第3 d开始迅速大幅下降;恢复投喂后各组纤维素酶和淀粉酶活力均有不同程度的升高,并在3 d内分别恢复到饥饿第1 d和第2 d的水平;试验期间各组蛤仔总抗氧化能力无显著变化(P<0.05)。
Keyword ：
再投喂 再投喂 总抗氧化能力 总抗氧化能力 消化酶活力 消化酶活力 菲律宾蛤仔 菲律宾蛤仔 饥饿 饥饿
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| GB/T 7714 | 李林明 , 王灿华 , 何亮华 . 饥饿对菲律宾蛤仔消化酶活力与抗氧化能力的影响 [J]. | 福建农业科技 , 2016 , (01) : 21-23 . |
| MLA | 李林明 et al. "饥饿对菲律宾蛤仔消化酶活力与抗氧化能力的影响" . | 福建农业科技 01 (2016) : 21-23 . |
| APA | 李林明 , 王灿华 , 何亮华 . 饥饿对菲律宾蛤仔消化酶活力与抗氧化能力的影响 . | 福建农业科技 , 2016 , (01) , 21-23 . |
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本研究分别测定了7种粒径的大黄鱼膨化颗粒饲料和5种粒径的大黄鱼软颗粒饲料入水后的溶胀率、溶失率和化学需氧量(COD),比较分析了这两种大黄鱼饲料的水中稳定性及对水质的影响。结果表明,浸泡1 h后膨化颗粒饲料溶胀率显著高于软颗粒饲料(P<0.01),溶失率显著低于软颗粒饲料(P<0.01);浸泡6 h时软颗粒饲料的COD值显著高于膨化颗粒饲料(P<0.01);这两类饲料的溶胀率、溶失率和COD值与浸泡时间、粒径大小均呈显著正相关。
Keyword ：
化学需氧量 化学需氧量 大黄鱼饲料 大黄鱼饲料 溶失率 溶失率 溶胀率 溶胀率
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| GB/T 7714 | 林旋 , 黄贞胜 , 王寿昆 et al. 大黄鱼膨化颗粒与软颗粒饲料浸泡时间对溶胀率、溶失率及COD的影响 [J]. | 福建水产 , 2015 , 37 (04) : 308-313 . |
| MLA | 林旋 et al. "大黄鱼膨化颗粒与软颗粒饲料浸泡时间对溶胀率、溶失率及COD的影响" . | 福建水产 37 . 04 (2015) : 308-313 . |
| APA | 林旋 , 黄贞胜 , 王寿昆 , 李林明 , 吴旭辉 , 郑少彬 . 大黄鱼膨化颗粒与软颗粒饲料浸泡时间对溶胀率、溶失率及COD的影响 . | 福建水产 , 2015 , 37 (04) , 308-313 . |
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