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学者姓名：汪世华
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Abstract ：
Moniliformin (MON) is a toxic secondary metabolite from Fusarium species. The natural contamination of MON in cereals and cereal by-products, poses a risk of exposure to MON. However, so far, no immunoassay method has been reported to detect MON in field samples. In this study, anti-MON monoclonal antibody (mAb) with high affinity (1 x 108) and isotype of IgG1 was subjected to establish indirect competitive ELISA (ic-ELISA) and immunochromatographic test strips (ICTS). The optimized ic-ELISA resulted in the lowest detection limit (LOD) of 1.55 mu g/mL. The LOD value for gold nanoparticles (AuNP) -based test strip was 0.83 mu g/mL. The multi branched gold nano-flower particles (AuNFs) reported higher sensitivity with LOD of 0.38 mu g/mL. Moreover, the developed test strips exhibited a high specificity without cross-reactivity to related competitive toxins. In conclusion, immunoassay methods were successfully developed to detect MON in field samples with a short detection time to ensure food security.
Keyword ：
ELISA ELISA Immunochromatographic test strip Immunochromatographic test strip Moniliformin Moniliformin Monoclonal antibody Monoclonal antibody
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| GB/T 7714 | Madushika, Lakshani , Huang, Yongming , Sun, Menghan et al. Development of sensitive and rapid immunoassays for Moniliformin (MON) detection based on nanomaterials labeled monoclonal antibodies [J]. | FOOD CHEMISTRY , 2025 , 472 . |
| MLA | Madushika, Lakshani et al. "Development of sensitive and rapid immunoassays for Moniliformin (MON) detection based on nanomaterials labeled monoclonal antibodies" . | FOOD CHEMISTRY 472 (2025) . |
| APA | Madushika, Lakshani , Huang, Yongming , Sun, Menghan , Liu, Yuxuan , Lu, Linfang , Li, Xiaoli et al. Development of sensitive and rapid immunoassays for Moniliformin (MON) detection based on nanomaterials labeled monoclonal antibodies . | FOOD CHEMISTRY , 2025 , 472 . |
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Flumequine (Flu) is a fluoroquinolone veterinary antibiotic, which is easy to accumulate in animals, and Flu residues may cause a latent risk to human physiological health. In this study, a high-affinity monoclonal antibody (2.09 x 109 M-1) to specifically recognize Flu (anti-Flu mAb) was prepared, and then the lateral flow immunochromatographic strips (LFIS) with excellent sensitivity and specificity were developed by combining mAbs with nanoparticles. The linear detection ranges of LFIS based on gold particles (AuNP-LFIS) and gold nanoflowers (AuNF-LFIS) were 1.95-250 ng/mL and 0.39-100 ng/mL, respectively, and two types of LFIS showed high sensitivity and accuracy in actual sample detection. It is noteworthy that the AuNF-LFIS exhibited a higher sensitivity compared to that of the AuNP-LFIS. The LFIS developed in this study were considered to have great application potential in monitoring food safety because of its superiorities including simple operation, high sensitivity, prompted speed and good specificity.
Keyword ：
Flumequine Flumequine Immunochromatographic strips Immunochromatographic strips Monoclonal antibodies Monoclonal antibodies Nanoparticles Nanoparticles
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| GB/T 7714 | Liu, Yuxuan , Jiang, Kang , Li, Xiaoli et al. Development of sensitive and specific immunochromatographic strips with nanoparticles for rapid detection of flumequine [J]. | FOOD CHEMISTRY-X , 2025 , 29 . |
| MLA | Liu, Yuxuan et al. "Development of sensitive and specific immunochromatographic strips with nanoparticles for rapid detection of flumequine" . | FOOD CHEMISTRY-X 29 (2025) . |
| APA | Liu, Yuxuan , Jiang, Kang , Li, Xiaoli , Lu, Linfang , Sun, Menghan , Li, Na et al. Development of sensitive and specific immunochromatographic strips with nanoparticles for rapid detection of flumequine . | FOOD CHEMISTRY-X , 2025 , 29 . |
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Due to the severe hazard of aflatoxins (AFs) to humans, it is of great significance to detect the key aflatoxins, aflatoxin B-1 (AFB(1)) and aflatoxin G(1) (AFG(1)), in food and feed in simple, rapid, and semi-quantitative ways. The hybridoma clone 3A1 was prepared in this study, and anti-AFB(1) monoclonal antibody (mAb) with high specificity and affinity (9.38 x 10(8) L/mol) from 3A1 was purified. The indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) demonstrated that the linear detection range for AFB(1) was 0.029-1.526 ng/mL with a limits of determination (LOD) of 0.023 ng/mL. A latex microsphere-based immunochromatographic test strip (LM-ICTS) was constructed based on 3A1, which showed that the strip could detect AFB(1) (LOD: lower than 1.79 ng/mL) and AFG(1) (LOD: lower than 8.08 ng/mL), and the linear detection ranges for AFB(1) and AFG(1) are 1.79-48.46 ng/mL and 8.08-107.40 ng/mL, respectively. The average recoveries of intra-assay and inter-assay for peanuts were (98.4 +/- 4.7)% and (92.6 +/- 7.6)%, and the average coefficient of variation (CVs) were 4.38% and 8.15%, respectively. For sunflower seeds, the intra-assay and inter-assay recoveries were (94.4 +/- 7.2)% and (89.2 +/- 4.3)%, and the average CVs were 6.6% and 4.9%, respectively. In summary, the developed LM-ICTS exhibited excellent sensitivity and specificity, which provided a rapidly stable on-site detection choice for AFB(1) and AFG(1) to contaminated agricultural samples, including grain and feed.
Keyword ：
AFB(1) AFB(1) AFG(1) AFG(1) hybridoma hybridoma immunochromatographic test strip immunochromatographic test strip latex microsphere latex microsphere
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| GB/T 7714 | Wang, Jie , Fu, Wangzhuo , Ma, Xuezhen et al. Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins [J]. | TOXINS , 2025 , 17 (9) . |
| MLA | Wang, Jie et al. "Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins" . | TOXINS 17 . 9 (2025) . |
| APA | Wang, Jie , Fu, Wangzhuo , Ma, Xuezhen , Chen, Lin , Song, Weitao , Ling, Sumei et al. Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins . | TOXINS , 2025 , 17 (9) . |
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Metabolomics, a critical tool for analyzing small-molecule metabolites, integrates with genomics, transcriptomics, and proteomics to provide a systems-level understanding of fungal biology. By mapping metabolic networks, it elucidates regulatory mechanisms driving physiological and ecological adaptations. In fungal pathogenesis, metabolomics reveals host-pathogen dynamics, identifying virulence factors like gliotoxin in Aspergillus fumigatus and metabolic shifts, such as glyoxylate cycle upregulation in Candida albicans. Ecologically, it highlights fungal responses to abiotic stressors, including osmolyte production like trehalose, enhancing survival in extreme environments. These insights highlight metabolomics' role in decoding fungal persistence and niche colonization. In drug discovery, it aids target identification by profiling biosynthetic pathways, supporting novel antifungal and nanostructured therapy development. Combined with multi-omics, metabolomics advances insights into fungal pathogenesis, ecological interactions, and therapeutic innovation, offering translational potential for addressing antifungal resistance and improving treatment outcomes for fungal infections. Its progress shed light on complex fungal molecular profiles, advancing discovery and innovation in fungal biology. The article explores how metabolomics, a new scientific field, uncovers previously hidden details about fungi, offering unprecedented insights into fungal biology.
Keyword ：
fungal infection fungal infection fungal pathogenesis fungal pathogenesis metabolites metabolites metabolomics metabolomics multi-omics multi-omics virulence virulence
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| GB/T 7714 | Adejor, John , Tumukunde, Elisabeth , Li, Guoqi et al. Stepping out of the dark: how metabolomics shed light on fungal biology [J]. | FEMS MICROBIOLOGY REVIEWS , 2025 , 49 . |
| MLA | Adejor, John et al. "Stepping out of the dark: how metabolomics shed light on fungal biology" . | FEMS MICROBIOLOGY REVIEWS 49 (2025) . |
| APA | Adejor, John , Tumukunde, Elisabeth , Li, Guoqi , Shehu, Tanimu Alhaji , Wu, Lihan , Jiang, Zhiwei et al. Stepping out of the dark: how metabolomics shed light on fungal biology . | FEMS MICROBIOLOGY REVIEWS , 2025 , 49 . |
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This study evaluates the effects of pyruvate decarboxylase and its lysine succinylation (Ksucc) on the development of Aspergillus flavus and the production of secondary metabolites. Mutant strains, including the knockout (triangle pdc1), pdc1 point mutants (K258R and K258E) and complementary (triangle pdc1.com) were constructed. The results showed that both Delta pdc1 and K258R strains exhibited decreased conidiophore and conidia production and failed to generate sclerotia. The production of aflatoxin B1 (AFB1) was significantly increased in the Delta pdc1 and K258R strains, while it decreased in the K258E strain. The results also indicate that pdc1 and its Ksucc are involved in the stress response and pathogenicity of A. flavus. Through GC-MS analysis, Delta pdc1 was found to produce several significantly decreased compounds, including n-hexadecanoic acid, 2-bromotetradecane, and palmitic acid, among others. Additionally, different volatile metabolites, such as 1-iodo-decane, n, n-dimethyloctanamide and n, n-dimethyl-7-octynamide, were not detected in the Delta pdc1 strain compared to WT and Delta pdc1.com. HPLC results showed that the production of pyruvic acid, malic acid and succinic acid increased in both the Delta pdc1 and K258R strains when compared to WT and Delta pdc1.com strains. Comparative RNA-seq analysis revealed a total of 3,817 differentially expressed genes (DEGs) including 1,913 up-regulated and 1,904 down-regulated genes in Delta pdc1 vs. WT. These results suggest that PDC1 and K258 play a crucial role in the biosynthesis of secondary metabolites, development and stress responses of A. flavus.
Keyword ：
aflatoxin aflatoxin Aspergillus flavus Aspergillus flavus pathogenicity pathogenicity Pyruvate decarboxylase Pyruvate decarboxylase secondary metabolites secondary metabolites
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| GB/T 7714 | Li, Guoqi , Tumukunde, Elisabeth , Chen, Yuanyuan et al. Pyruvate decarboxylase and its lysine succinylation contribute to the development and metabolite biosynthesis in pathogenic fungus Aspergillus flavus [J]. | MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY , 2025 . |
| MLA | Li, Guoqi et al. "Pyruvate decarboxylase and its lysine succinylation contribute to the development and metabolite biosynthesis in pathogenic fungus Aspergillus flavus" . | MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY (2025) . |
| APA | Li, Guoqi , Tumukunde, Elisabeth , Chen, Yuanyuan , Zhang, Yao , Kuang, Yunlu , Adejor, John et al. Pyruvate decarboxylase and its lysine succinylation contribute to the development and metabolite biosynthesis in pathogenic fungus Aspergillus flavus . | MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY , 2025 . |
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Aspergillus flavus and its secondary metabolites, aflatoxins (AFs), especially aflatoxin B1 (AFB1), seriously affect agricultural production, food storage, and human health. Succinyl-CoA synthase ADP-forming subunit beta (SCS) is involved in the synthesis of succinate from succinyl-CoA in the tricarboxylic acid cycle. In this study, we demonstrated that SCS led to decreased aflatoxin production. Bioassay results showed that deletion of sucB (the gene coding for SCS) led to increased succinyl-CoA accumulation. Catalyzed by succinyl transferase (STA), the increased amount of succinyl-CoA in Delta sucB leads to increased levels of global protein succinylation, which causes upregulation of AFB1 accumulation in Delta sucB. To elucidate the mechanism of increased AFB1 accumulation in Delta sucB, the relevant enzymes and metabolites involved in the aflatoxin biosynthesis pathway were examined through proteome and metabolome analyses. These data illustrate that the deletion of sucB results in an increase in (1'S, 5'S) - averufin catalyzed by AflK, (1'S)-averantin catalyzed by AflD, and aflatoxin G2/O- methylsterigmatocystin catalyzed by AflP. We also found that AflM is not only upregulated but also succinylated in Delta sucB; Ach1 (acetyl-CoA hydrolase, Ach1) is downregulated in Delta sucB and interacts with SCS. Therefore, we deduce a pathway of Ach1/STA-SCS-succinylated AflM for AFB1 biosynthesis, which provides knowledge for the control of A. flavus and AFs.
Keyword ：
aflatoxin B1 aflatoxin B1 Aspergillus flavus Aspergillus flavus development development succinylation level succinylation level succinyl-CoA synthetase succinyl-CoA synthetase
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| GB/T 7714 | Xie, Rui , Zhuang, Zhenhong , Chen, Qionghui et al. STA regulates succinylated AflM triggered by SCS to contribute to aflatoxin biosynthesis through the Ach1 [J]. | VIRULENCE , 2025 , 16 (1) . |
| MLA | Xie, Rui et al. "STA regulates succinylated AflM triggered by SCS to contribute to aflatoxin biosynthesis through the Ach1" . | VIRULENCE 16 . 1 (2025) . |
| APA | Xie, Rui , Zhuang, Zhenhong , Chen, Qionghui , Xie, Chunlan , Adejor, John , Nie, Xinyi et al. STA regulates succinylated AflM triggered by SCS to contribute to aflatoxin biosynthesis through the Ach1 . | VIRULENCE , 2025 , 16 (1) . |
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本发明公开了一种检测氟甲喹的金纳米花免疫层析试纸条及其制备方法。本发明筛选了一株小鼠杂交瘤细胞3B4，保藏编号为CGMCC No.46134，采用该小鼠杂交瘤细胞3B4分泌得到抗氟甲喹单克隆抗体，并用该抗氟甲喹单克隆抗体制备金纳米花免疫探针，最后制备得到检测氟甲喹的金纳米花免疫层析试纸条。本发明制备的金纳米花免疫层析试纸条测定时间短且易于操作，成本低，具有优异的特异性和灵敏度，可以简便、快速地检测氟甲喹。
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| GB/T 7714 | 汪世华 , 刘宇轩 , 凌素美 et al. 一种检测氟甲喹的金纳米花免疫层析试纸条及其制备方法 : CN202510165379.X[P]. | 2025-02-14 . |
| MLA | 汪世华 et al. "一种检测氟甲喹的金纳米花免疫层析试纸条及其制备方法" : CN202510165379.X. | 2025-02-14 . |
| APA | 汪世华 , 刘宇轩 , 凌素美 , 孙梦晗 , 蒋康 , 李娜 et al. 一种检测氟甲喹的金纳米花免疫层析试纸条及其制备方法 : CN202510165379.X. | 2025-02-14 . |
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| GB/T 7714 | Ahmad, Tanvir , Wang, Shihua , Liu, Yang . Aspergillus flavus and Aflatoxins (3rd Edition) [J]. | TOXINS , 2025 , 17 (7) . |
| MLA | Ahmad, Tanvir et al. "Aspergillus flavus and Aflatoxins (3rd Edition)" . | TOXINS 17 . 7 (2025) . |
| APA | Ahmad, Tanvir , Wang, Shihua , Liu, Yang . Aspergillus flavus and Aflatoxins (3rd Edition) . | TOXINS , 2025 , 17 (7) . |
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为解决我国东北高寒地区低温环境下玉米秸秆不易降解的难题，利用平板快速筛选方法结合低温发酵培养对耐低温生长的45个真菌菌株进行筛选，获得能够在低温条件下同时产生漆酶、纤维素酶、半纤维素酶及过氧化物酶等多种木质素纤维素酶的6株真菌菌株，并对该6株菌株进行玉米秸秆低温腐解效果的评价，分别在处理10,20,30,40,50 d时测定其产生的木质素纤维素酶活性及其玉米秸秆中各组分的降解率。结果发现：在20℃条件下进行液体发酵培养10 d后，这些菌株产生的漆酶活性可达7.47 U/mL(L2431)和7.2 U/mL(X1957)，纤维素酶活可达239 U/mL (L743)和167 U/mL(L1675)，半纤维素酶活可达179.3 U/mL(YF410)、120.0 U/mL (H382)和113.3 U/mL (L2431)，过氧化物酶活可达为25.1 U/mL(L2431-3)、24.9 U/mL(2423)和15.9 U/mL(L2419)。6种菌株在低温条件下产生各种木质素纤维素酶活性达到最大值的时间不完全一致，且对玉米秸秆各组分的降解率存在着显著的差异（P<0.05），但对玉米秸秆组分的总降解率均随着时间的延长而增加，在处理50 d后的木质素降解率为18.2%～25.1%，纤维素降解率为24.1%～44.6%，半纤维素降解率为19.3%～26.3%，玉米秸秆总降解率为61.8%～87.0%。本研究筛选到在低温环境下生长较快且产酶能力较强和高效腐解玉米秸秆的真菌菌株，对我国东北寒冷地区玉米秸秆还田措施具有积极的推动作用和应用前景。
Keyword ：
平板筛选 平板筛选 木质纤维素酶 木质纤维素酶 玉米秸秆 玉米秸秆 真菌菌株 真菌菌株 降解 降解
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| GB/T 7714 | 杨会敏 , 李伟 , 汪世华 et al. 高效腐解玉米秸秆耐低温真菌菌株的筛选及评价 [J]. | 菌物研究 , 2025 , 23 (03) : 211-222 . |
| MLA | 杨会敏 et al. "高效腐解玉米秸秆耐低温真菌菌株的筛选及评价" . | 菌物研究 23 . 03 (2025) : 211-222 . |
| APA | 杨会敏 , 李伟 , 汪世华 , 尹文兵 . 高效腐解玉米秸秆耐低温真菌菌株的筛选及评价 . | 菌物研究 , 2025 , 23 (03) , 211-222 . |
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Pathogenic filamentous fungi pose a significant threat to global food security and human health. The limitations of available antifungal agents, including resistance and toxicity, highlight the need for developing innovative antifungal strategies. Antifungal proteins (AFPs) are a class of secreted small proteins that exhibit potent antifungal activity against filamentous fungi, yet the underlying mechanism remains partially understood. In this study, we investigate the molecular and cellular effects of two AFPs, PgAFP and AfAFP, on Aspergillus flavus, a representative filamentous fungus. These AFPs affect various fungal phenotypes and exert an intracellular effect by interacting with Ntp1, a fungi exclusive protein modulating diverse fungal traits. We find that Ntp1 amino acids 417-588 are critical for AFP binding and play a role in regulating growth, development, sporulation, sclerotia formation, toxin synthesis, and pathogenicity. Results generated from this study will help to control pathogenic fungi.
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| GB/T 7714 | Wang, Yu , Wang, Sen , Chen, Yuanyuan et al. The role of Npt1 in regulating antifungal protein activity in filamentous fungi [J]. | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| MLA | Wang, Yu et al. "The role of Npt1 in regulating antifungal protein activity in filamentous fungi" . | NATURE COMMUNICATIONS 16 . 1 (2025) . |
| APA | Wang, Yu , Wang, Sen , Chen, Yuanyuan , Xie, Chunlan , Xu, Haibo , Lin, Yunhua et al. The role of Npt1 in regulating antifungal protein activity in filamentous fungi . | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
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