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学者姓名：李兆伟
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Abstract ：
The root is one of the most important organs that determines the final grain yield in rice. Auxin is essential for root development in plants. Rice auxin response factor7 (OsARF7), belonging to the ARF family, is a key regulator of root development. Here, we show that OsARF7 positively regulates root development via auxin signaling. The osarf7 mutants display a significant decrease in the root number, adventitious root (AR) number and length, and primary root (PR) length, compared with the wild-type. Exogenous NAA treatment significantly suppresses PR length in osarf7 mutants, OsARF7-OE lines, and its wild-type, does not affect the root number of osarf7 mutants, but suppresses the biomass of osarf7 mutants. At the molecular level, OsARF7 is preferentially expressed in the culm, root, and leaf, especially highly expressed in the tips of the PR, AR, root pericycle, and lateral root (LR) primordia; meanwhile, OsARF7 expression is significantly enhanced by exogenous NAA treatment, suggesting that the positive regulatory role of OsARF7 on root development is based on auxin signaling. A series of biochemical and genetic analyses demonstrate that OsARF7 functions upstream of OsCRL1 and acts downstream of OsMADS25 to regulate root development via auxin signaling. To conclude, OsARF7 is a key positive regulatory factor that regulates root development by activating the expression of OsCRL1 via auxin signaling, by which, OsMADS25 positively mediates OsARF7 expression in rice. This work provides valuable insight into the regulatory mechanism controlling root development and a genetic resource for the molecular improvement of root architecture.
Keyword ：
Auxin signaling Auxin signaling OsARF7 OsARF7 OsCRL1 OsCRL1 Rice Rice Root development Root development
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| GB/T 7714 | Sun, Congying , Fan, Kai , Wang, Xin et al. The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.) [J]. | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
| MLA | Sun, Congying et al. "The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.)" . | PLANT MOLECULAR BIOLOGY 115 . 2 (2025) . |
| APA | Sun, Congying , Fan, Kai , Wang, Xin , Liu, Honghai , Guo, Nuoping , Liu, Wanyu et al. The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.) . | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
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Polyphenol oxidase (PPO) oxidizes polyphenols, causing browning in tobacco (Nicotiana tabacum L.) leaves during roasting, which reduces their quality and commercial value. In this study, 13 NtPPO genes were identified through genome mining, and their bioinformatic analyses were performed, including gene structure, protein structure, and physicochemical properties. Tissue-specific expression analysis found that the NtPPO genes were substantially expressed in leaves, whereas NtPPO9 and NtPPO10 were also highly expressed in flowers. During leaf growth, NtPPO1, 3, 5, 6, and 7 expression levels changed dramatically, whereas NtPPO2, 9, 10, and 13 expression patterns remained steady. Notably, NtPPO1 displayed the highest expression levels during the maturity stage of tobacco leaves. Subcellular localization revealed that NtPPO1, 6, 9, and 12 were primarily distributed in chloroplasts. KO-87 was generated through CRISPR/Cas9 technology, including mutations in the NtPPO1, 2, 4, 6, 8, 9, 11, 12, and 13 genes. KO-87 exhibits similar growth to the wild-type, but with decreased PPO activity in leaves and browning levels after roasting. Metabolomics analysis indicated that polyphenol metabolites were significantly upregulated, while quinone metabolites were significantly downregulated in the leaves of mutant plants. This study systematically explored the functions of NtPPO genes, providing preliminary insights into their key roles in the browning process of tobacco leaves. These findings offer new research directions and potential solutions for addressing the browning problem during tobacco leaf roasting.
Keyword ：
Browning of tobacco leaves Browning of tobacco leaves CRISPR/Cas9 CRISPR/Cas9 NtPPOs NtPPOs Tobacco Tobacco
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| GB/T 7714 | Zhang, Shixu , Lin, Yingchao , Zeng, Xinhai et al. Genome-wide characterization of polyphenol oxidase (PPO) gene family in tobacco and the molecular improvement of browning reaction for K326 tobacco leaves [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 235 . |
| MLA | Zhang, Shixu et al. "Genome-wide characterization of polyphenol oxidase (PPO) gene family in tobacco and the molecular improvement of browning reaction for K326 tobacco leaves" . | INDUSTRIAL CROPS AND PRODUCTS 235 (2025) . |
| APA | Zhang, Shixu , Lin, Yingchao , Zeng, Xinhai , Liu, Guangju , Ye, Guixiang , Du, Changyang et al. Genome-wide characterization of polyphenol oxidase (PPO) gene family in tobacco and the molecular improvement of browning reaction for K326 tobacco leaves . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 235 . |
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In response to the severe continuous cropping obstacles encountered during the cultivation of passion fruit, which leads to significant declines in yield and quality, as well as rampant pests and diseases, it is particularly important to explore strategies for mitigating these obstacles. The present study used the rhizosphere soil samples from one-year-old (FY, first-year cropping) and two-year-old (SY, second-year cropping) "Golden Passion Fruit" plants, along with soil from uncropped land (CK) as a control and the techniques such as high-throughput sequencing, qRT-PCR, and HPLC-MS to analyze the main physicochemical properties, phenolic acid content, and microbial community changes in the rhizosphere soil of passion fruit under different continuous cropping durations, the results indicated that the contents of total nitrogen, total phosphorus, available nitrogen, available phosphorus, available potassium, and organic matter in FY soil were significantly higher than those in SY soil, and the pH value of the FY soil was also significantly higher than that of SY soil. Additionally, compared with FY soil, the activities of polyphenol oxidase, peroxidase, urease, and invertase in SY soil were significantly reduced by 18.0%, 43.6%, 19.8%, and 45.5%, respectively. HPLC analysis revealed that the concentrations of syringic acid, vanillin, benzoic acid, and ferulic acid in the SY soil were significantly increased by 18.0%, 21.9%, 24.4%, and 21.1%, respectively, compared to those in the FY soil. qRT-PCR analysis showed that as the duration of continuous cropping increased, the total number of bacteria in the rhizosphere soil of passion fruit decreased by 9.37%, while the total number of fungi increased by 57.8%. High-throughput sequencing results demonstrated that at the genus level, the relative abundances of Acidothermus, Acidibacter, Bacillus, and Acidobacterium were significantly increased by38.0%, 56.3%, 34.3%, 77.3%. whereas the relative abundances of Rhizomicrobium, Nitrospira, Burkholderia, Sphingomonas, Gemmatimonas, Streptomyces, and Nocardioides were significantly lower 45.2%,59.3%,50.6%,89.1%,74.5%,82.7% in the SY soil, relative to those in the FY soil. In summary, as the duration of continuous cropping increases, the soil fertility, enzyme activity, pH value, and beneficial microbial content in the rhizosphere of passion fruit decrease significantly, while the contents of phenolic acids and pathogenic microorganisms increase significantly. The findings of this study provide a theoretical basis for further elucidating the formation mechanism and mitigation strategies of continuous cropping obstacles in passion fruit.
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| GB/T 7714 | Lin, Weiwei , Chen, Zhihan , Li, Zhaowei et al. Changes in enzyme activity and microbial community of rhizosphere soil under continuously monocultured Passiflora edulis treatment [J]. | PLOS ONE , 2025 , 20 (7) . |
| MLA | Lin, Weiwei et al. "Changes in enzyme activity and microbial community of rhizosphere soil under continuously monocultured Passiflora edulis treatment" . | PLOS ONE 20 . 7 (2025) . |
| APA | Lin, Weiwei , Chen, Zhihan , Li, Zhaowei , Lin, Wenxiong . Changes in enzyme activity and microbial community of rhizosphere soil under continuously monocultured Passiflora edulis treatment . | PLOS ONE , 2025 , 20 (7) . |
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【目的】从睡莲基因组中鉴定NAC成员，并对其进化历程以及在不同组织和ABA胁迫下的表达模式进行分析，为睡莲NAC家族的分子进化提供理论基础，同时为睡莲NAC成员调控花器官形态建成和响应逆境胁迫提供候选基因。【方法】利用全基因组方法分析睡莲NAC家族成员的保守基序、基因结构、系统进化、染色体定位、基因复制事件、启动子顺式调控元件，并使用转录组和RT-qPCR对睡莲NAC家族成员在不同组织和ABA胁迫下的表达模式进行分析。【结果】从睡莲基因组中筛选出64个NAC成员，可分为15个亚家族。NcNAC成员不均匀地分布在睡莲14条染色体上，大部分成员定位于1、2、9和11号染色体上。睡莲NAC家族的片段复制事件与睡莲NAC家族的扩增紧密联系。NcNAC11、NcNAC43、NcNAC36和NcNAC52在花器官间的表达量较高，推断这些基因可能参与睡莲花器官的形态建成。NcNAC成员启动子区含有大量与胁迫响应相关的顺式调控元件，其中启动子区含有ABA响应元件最多的NcNAC07、NcNAC08、NcNAC34和NcNAC43能够响应ABA胁迫，表明NcNAC07、NcNAC08、NcNAC34和NcNAC43可能与睡莲的抗逆胁迫有关。【结论】在睡莲中有64个NcNAC成员，可以将其分为15个亚家族，NcNAC11、NcNAC43、NcNAC36和NcNAC52在花器官中的表达量较高，NcNAC07、NcNAC08、NcNAC34和NcNAC43能够响应ABA胁迫。
Keyword ：
NAC家族 NAC家族 分子进化 分子进化 睡莲 睡莲 表达分析 表达分析 转录因子 转录因子
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| GB/T 7714 | 钱政毅 , 吴绍芳 , 曹舒怡 et al. 睡莲NAC转录因子的鉴定及其表达分析 [J]. | 生物技术通报 , 2025 , 41 (02) : 234-247 . |
| MLA | 钱政毅 et al. "睡莲NAC转录因子的鉴定及其表达分析" . | 生物技术通报 41 . 02 (2025) : 234-247 . |
| APA | 钱政毅 , 吴绍芳 , 曹舒怡 , 宋雅欣 , 潘鑫峰 , 李兆伟 et al. 睡莲NAC转录因子的鉴定及其表达分析 . | 生物技术通报 , 2025 , 41 (02) , 234-247 . |
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为探索高效纤维素降解菌在农业领域应用研究的新思路，通过检索分析近年来纤维素降解菌筛选及产酶优化相关研究的文献，从菌株种类、产酶条件优化研究、复合菌（群）构建和菌（群）在农业应用等4个方面进行了总结，归纳了不同种类菌株筛选策略和优势特点，分析了纤维素降解菌产酶条件优化的原料种类及菌株的各异性，阐述了复合菌（群）构建的必要性及其重要意义，综合概括了纤维素降解菌（群）在生物肥料、农作物废弃物、生物能源三个方面的应用概况。提出当前研究在单一菌体所产纤维素酶种类单一、复合菌（群）产酶条件优化等方面存在不足，提出加强高效纤维素降解菌筛选、利用分子生物学技术构建纤维素降解菌的基因库以及降解机制研究等建议，以期为提高纤维素降解菌筛选效率，减少农业废弃物资源浪费，促进农业快速发展和资源循环利用提供理论支撑。
Keyword ：
农业废弃物 农业废弃物 协同效应 协同效应 复合体系构建 复合体系构建 微生物 微生物 生物降解 生物降解 纤维素酶 纤维素酶 纤维素降解菌 纤维素降解菌 菌群 菌群
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| GB/T 7714 | 张晓杰 , 陈俊玲 , 王莎莎 et al. 纤维素降解菌筛选与应用研究进展 [J]. | 农学学报 , 2025 , 15 (06) : 50-56 . |
| MLA | 张晓杰 et al. "纤维素降解菌筛选与应用研究进展" . | 农学学报 15 . 06 (2025) : 50-56 . |
| APA | 张晓杰 , 陈俊玲 , 王莎莎 , 李艳春 , 李兆伟 , 王义祥 . 纤维素降解菌筛选与应用研究进展 . | 农学学报 , 2025 , 15 (06) , 50-56 . |
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Cotton diseases pose a serious threat to yield and fiber quality. Systemic acquired resistance (SAR) is a crucial and prolonged immune response to pathogen infection; yet, its regulatory mechanisms remain poorly understood in cotton. Here, 35 TGACG binding (TGA) transcription factors were identified in Gossypium hirsutum, classifying them into five groups based on phylogenetic analysis, conserved motifs, and gene structures. Among them, GhTGA12, a group II member, emerged as a novel disease-associated transcription factor based on RNA-seq analysis under biotic stress conditions. GhTGA12 encodes a nuclear protein with 11 exons and 10 introns, harboring the conserved TGA-specific domains (bZIP, DOG1, Q1, and Q2). GhTGA12 has transcriptional activation activity through its N-terminal region and could be triggered by Pst DC3000. While GhTGA12-over-expressing Arabidopsis did not exhibit enhanced resistance after a single Pst DC3000 challenge, it showed significantly increased resistance upon Pst DC3000 pretreatment, consistent with SAR activation. These lines also exhibited elevated ROS levels and increased PR expression levels (PR1, PR2, PR5, and GRXS13). Additionally, GhTGA12 physically interacts with GhNPR1, a key regulator of SAR. Collectively, this study identifies GhTGA12 as a critical SAR-positive regulator via ROS signaling, PR gene activation, and GhNPR1 interaction, offering new targets for enhancing disease resistance in cotton.
Keyword ：
Cotton Cotton GhTGA12 GhTGA12 Integrated genome and expression Integrated genome and expression Systemic acquired resistance Systemic acquired resistance TGA TGA
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| GB/T 7714 | Qian, Zhengyi , Cao, Shuyi , Jia, Qi et al. Integrated genome and expression of the TGA family in Gossypium hirsutum reveals GhTGA12 could positively regulate systemic acquired resistance [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 236 . |
| MLA | Qian, Zhengyi et al. "Integrated genome and expression of the TGA family in Gossypium hirsutum reveals GhTGA12 could positively regulate systemic acquired resistance" . | INDUSTRIAL CROPS AND PRODUCTS 236 (2025) . |
| APA | Qian, Zhengyi , Cao, Shuyi , Jia, Qi , Zhang, Xiangyu , Du, Xinling , Su, Yina et al. Integrated genome and expression of the TGA family in Gossypium hirsutum reveals GhTGA12 could positively regulate systemic acquired resistance . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 236 . |
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To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased under the drought stresses, suggesting a decrease in the efficiency of conversion by light to electricity in the PSII reaction center. SOD and CAT activities were elevated during the early stage of drought stresses but sharply declined during the later stage of drought stresses. POD enzyme activity was less affected by moderate drought stress, but it was inhibited by severe drought stress. Additionally, moderate drought stress increased the SPS activity and reduced GS activity in K326 tobacco leaves in the later growth stage, indicating that moderate drought stress can promote the transduction from nitrogen metabolism to carbon metabolism in tobacco leaves in the later growth stage, which will help to improve the internal chemical quality of tobacco leaves.
Keyword ：
carbon and nitrogen metabolism carbon and nitrogen metabolism chlorophyll chlorophyll drought stress drought stress Flue-cured tobacco Flue-cured tobacco
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| GB/T 7714 | Wei, Kesu , Liu, Guangju , Wei, Bin et al. Effects of Drought Stress on the Physiological Characteristics of Flue-Cured Tobacco during the Vigorous Growing Period [J]. | PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY , 2025 , 94 (4) : 1287-1298 . |
| MLA | Wei, Kesu et al. "Effects of Drought Stress on the Physiological Characteristics of Flue-Cured Tobacco during the Vigorous Growing Period" . | PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY 94 . 4 (2025) : 1287-1298 . |
| APA | Wei, Kesu , Liu, Guangju , Wei, Bin , Zhang, Qifang , Wu, Shengjiang , Li, Zhaowei . Effects of Drought Stress on the Physiological Characteristics of Flue-Cured Tobacco during the Vigorous Growing Period . | PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY , 2025 , 94 (4) , 1287-1298 . |
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Drought stress is a major limit on the maize growth and productivity, and understanding the drought response mechanism is one of the important ways to improve drought resistance in maize. However, more drought-related genes and their regulated mechanisms are still to be reported. Here, we identified a novel NAC transcription factor ZmNAC55 in Zea mays and comprehensively investigated the functions of ZmNAC55 under drought stress. ZmNAC55 belonged to the NAP subfamily. ZmNAC55 had a conserved NAC domain in the N-terminal region and a divergent TAR region in the C-terminal region. ZmNAC55 was a nuclear protein, and ZmNAC55 and its TAR region had the transcriptional activation activity. Furthermore, the expression level of ZmNAC55 in leaves could be highly induced by drought stress. ZmNAC55 overexpression in Arabidopsis conferred the drought-sensitive phenotype with higher water loss, lower survival rate, higher membrane ion leakage, and higher expression levels of some drought-related genes. Meanwhile, ZmNAC55 underexpression in maize enhanced drought tolerance with lower water loss, higher survival rate, lower membrane ion leakage and lower expression levels of some drought-related genes. In addition, ZmNAC55 appeared to be very key in regulating ROS production under drought stress. Moreover, ZmNAC55 could activate ZmHOP3 expression by binding to its promoter. A novel working model of ZmNAC55 under drought stress could be found in maize. Taken together, the NAC transcription factor ZmNAC55 could negatively regulate drought stress via increasing ZmHOP3 expression in maize. ZmNAC55 is a promising candidate for improving drought resistance in maize.
Keyword ：
Drought stress Drought stress Maize Maize Molecular mechanism Molecular mechanism NAC transcription factor NAC transcription factor ZmNAC55 ZmNAC55
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| GB/T 7714 | Fan, Kai , Wu, Yuchen , Mao, Zhijun et al. A novel NAC transcription factor ZmNAC55 negatively regulates drought stress in Zea mays [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2024 , 214 . |
| MLA | Fan, Kai et al. "A novel NAC transcription factor ZmNAC55 negatively regulates drought stress in Zea mays" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 214 (2024) . |
| APA | Fan, Kai , Wu, Yuchen , Mao, Zhijun , Yin, Kan , He, Yuxi , Pan, Xinfeng et al. A novel NAC transcription factor ZmNAC55 negatively regulates drought stress in Zea mays . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2024 , 214 . |
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Key message Rice OsSPL11 activates the expression ofGW5Lthrough binding to its promoter and positively regulates grain size. Grain size (GS) is an important determinant of grain weight and yield potential in cereal. Here, we report the functional analysis of OsSPL11 in grain length (GL), grain width (GW), and 1000-grain weight (TGW). OsSPL11 mutant plants, osspl11 lines, exhibited a decrease in GL, GW, and TGW, and OsSPL11-OE lines showed an increase in GL and TGW. Expression analysis revealed that OsSPL11 was located in the nucleus and highly expressed in spikelet hull and young development grains, consistent with its function in determining GS. Further analysis confirmed that OsSPL11 directly activates the expression of GW5L to regulate GS, meanwhile OsSPL11 expression is negatively regulated by OsGBP3. Taken together, our findings demonstrate that OsSPL11 could be a key regulator of affecting GS during the spikelet hull development and facilitate the process of improving grain yield by GS modification in rice.
Keyword ：
Grain size Grain size OsGW5L OsGW5L OsSPL11 OsSPL11 Rice Rice
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| GB/T 7714 | Zeng, Xinhai , Fan, Kai , Shi, Yu et al. OsSPL11 positively regulates grain size by activating the expression of GW5L in rice [J]. | PLANT CELL REPORTS , 2024 , 43 (9) . |
| MLA | Zeng, Xinhai et al. "OsSPL11 positively regulates grain size by activating the expression of GW5L in rice" . | PLANT CELL REPORTS 43 . 9 (2024) . |
| APA | Zeng, Xinhai , Fan, Kai , Shi, Yu , Chen, Rui , Liu, Wanyu , Wang, Xin et al. OsSPL11 positively regulates grain size by activating the expression of GW5L in rice . | PLANT CELL REPORTS , 2024 , 43 (9) . |
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The small heat shock proteins (sHSPs) are important components in plant growth and development, and stress response. However, a systematical understanding of the sHSP family is yet to be reported in five diploid Gossypium species. In this study, 34 GlsHSPs, 36 GrsHSPs, 37 GtsHSPs, 37 GasHSPs, and 38 GhesHSPs were identified in Gossypium longicalyx, Gossypium raimondii, Gossypium turneri, Gossypium arboreum, and Gossypium herbaceum, respectively. These sHSP members can be clustered into 10 subfamilies. Different subfamilies had different member numbers, motif distributions, gene structures, gene duplication events, gene loss numbers, and cis-regulatory elements. Besides, the paleohexaploidization event in cotton ancestor led to expanding the sHSP members and it was also inherited by five diploid Gossypium species. After the cotton ancestor divergence, the sHSP members had the relatively conserved evolution in five diploid Gossypium species. The comprehensive evolutionary history of the sHSP family was revealed in five diploid Gossypium species. Furthermore, several GasHSPs and GhesHSPs were important candidates in plant growth and development, and stress response. These current findings can provide valuable information for the molecular evolution and further functional research of the sHSP family in cotton.
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| GB/T 7714 | Fan, Kai , Qian, Zhengyi , He, Yuxi et al. Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species [J]. | PLANT GENOME , 2024 , 17 (3) . |
| MLA | Fan, Kai et al. "Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species" . | PLANT GENOME 17 . 3 (2024) . |
| APA | Fan, Kai , Qian, Zhengyi , He, Yuxi , Chen, Jiayuan , Ye, Fangting , Zhu, Xiaogang et al. Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species . | PLANT GENOME , 2024 , 17 (3) . |
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