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学者姓名：张永强
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Abstract ：
Phosphorus (P) is a crucial element for plant growth and development, with inorganic phosphate (Pi) being the primary form absorbed by plant roots. However, soil often experiences Pi deficiency, leading to early leaf senescence and hastening the P re-mobilization from senescent leaves to young tissues. The PHOSPHATE STARVATION RESPONSE (PHR) proteins are widely acknowledged as pivotal regulatory factors in response to low Pi, and they may be involved in the regulation of leaf senescence and P remobilization triggered by Pi deprivation. Nevertheless, the mechanisms underlying the regulation of these processes require further elucidation. In this study, it was observed that overexpression of AtPHR1 accelerates leaf senescence, facilitates P transfer, and upregulates the expression of S-like RNase-encoding genes such as RNS1, RNS2, and RNS5. Subsequent investigations utilizing yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and transient transcription expression assay (TTEA) revealed direct binding of AtPHR1 to the P1BS motifs within the promoters of these genes, thereby activating their transcription. Similarly, the overexpression of tomato PHR protein SlPHL1 also increased the transcript levels of the S-like RNase-encoding genes including LE and LX. Moreover, experiments using Y1H, EMSA, and TTEA confirmed that SlPHL1 directly binds to the promoters of these two genes through P1BS motifs and subsequently activates their transcription. Additionally, introducing SlPHL1 into Arabidopsis led to an enhanced P turnover and RNA degradation, along with an increase in the expression of RNS1 and RNS2. Further investigations revealed that similar to AtPHR1, SlPHL1 is capable of directly activating the transcription of RNS1 and RNS2. Taken together, these findings suggest that AtPHR1 and SlPHL1 play a pivotal role in facilitating P remobilization by directly stimulating the transcription of S-like RNase-encoding genes. This mechanism appears to be conserved across Arabidopsis and tomato, highlighting the importance of PHRs in optimizing plant P utilization.
Keyword ：
Arabidopsis Arabidopsis AtPHR1 AtPHR1 Phosphate starvation Phosphate starvation Phosphorus remobilization Phosphorus remobilization S-like RNase S-like RNase SlPHL1 SlPHL1 Solanum lycopersicum Solanum lycopersicum
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| GB/T 7714 | Li, Chengquan , Huang, Shaoxuan , Zhang, Duanmei et al. AtPHR1 and SlPHL1 positively regulate phosphorus remobilization in response to phosphate starvation by directly facilitating the transcription of genes encoding S-like ribonucleases in Arabidopsis and tomato, respectively [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 221 . |
| MLA | Li, Chengquan et al. "AtPHR1 and SlPHL1 positively regulate phosphorus remobilization in response to phosphate starvation by directly facilitating the transcription of genes encoding S-like ribonucleases in Arabidopsis and tomato, respectively" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 221 (2025) . |
| APA | Li, Chengquan , Huang, Shaoxuan , Zhang, Duanmei , Zhu, Lin , Chen, Mingxue , Liu, Yanan et al. AtPHR1 and SlPHL1 positively regulate phosphorus remobilization in response to phosphate starvation by directly facilitating the transcription of genes encoding S-like ribonucleases in Arabidopsis and tomato, respectively . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 221 . |
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Increased acid phosphatase (APase) activity is a prominent feature of tomato (Solanum lycopersicum) responses to inorganic phosphate (Pi) restriction. SlPHL1, a phosphate starvation response (PHR) transcription factor, has been identified as a positive regulator of low Pi (LP)-induced APase activity in tomato. However, the molecular mechanism underlying this regulation remains to be elucidated. Here, SlPHL1 was found to positively regulate the LP-induced expression of five potential purple acid phosphatase (PAP) genes, namely SlPAP7, SlPAP10b, SlPAP12, SlPAP15, and SlPAP17b. Furthermore, we provide evidence that SlPHL1 can stimulate transcription of these five genes by binding directly to the PHR1 binding sequence (P1BS) located on their promoters. The P1BS mutation notably weakened SlPHL1 binding to the promoters of SlPAP7, SlPAP12, and SlPAP17b but almost completely abolished SlPHL1 binding to the promoters of SlPAP10b and SlPAP15. As a result, the transcriptional activation of SlPHL1 on SlPAP10b and SlPAP15 was substantially diminished. In addition, not only did transient overexpression of either SlPAP10b or SlPAP15 in tobacco leaves increase APase activity, but overexpression of SlPAP15 in Arabidopsis and tomato also increased APase activity and promoted plant growth. Subsequently, two SPX proteins, SlSPX1 and SlSPX4, were shown to physically interact with SlPHL1. Moreover, SlSPX1 inhibited the transcriptional activation of SlPHL1 on SlPAP10b and SlPAP15 and negatively regulated the activity of APase. Taken together, these results demonstrate that SlPHL1-mediated LP signaling promotes APase activity by activating the transcription of SlPAP10b and SlPAP15, which may provide valuable insights into the mechanisms of tomato response to Pi-limited stress.
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| GB/T 7714 | Liu, Yanan , Li, Chengquan , Zhang, Duanmei et al. SlPHL1 positively modulates acid phosphatase in response to phosphate starvation by directly activating the genes SlPAP10b and SlPAP15 in tomato [J]. | PHYSIOLOGIA PLANTARUM , 2024 , 176 (1) . |
| MLA | Liu, Yanan et al. "SlPHL1 positively modulates acid phosphatase in response to phosphate starvation by directly activating the genes SlPAP10b and SlPAP15 in tomato" . | PHYSIOLOGIA PLANTARUM 176 . 1 (2024) . |
| APA | Liu, Yanan , Li, Chengquan , Zhang, Duanmei , Huang, Shaoxuan , Wang, Yi , Wang, Enhui et al. SlPHL1 positively modulates acid phosphatase in response to phosphate starvation by directly activating the genes SlPAP10b and SlPAP15 in tomato . | PHYSIOLOGIA PLANTARUM , 2024 , 176 (1) . |
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Nitrogen (N) and phosphorus (P), as indispensable mineral elements, both play pivotal roles in plant growth and development. Despite the intimate association between nitrate signaling and inorganic phosphate (Pi) signaling, the regulatory function of Pi in N metabolism remains poorly understood. In this study, we observed that Pi deficiency leads to a reduction in the activity of nitrate reductase (NR), an essential enzyme involved in N metabolism. Furthermore, PHOSPHATE STARVATION RESPONSE 1 (PHR1), a key regulator of Pi signaling, exerts a negative impact on both NR activity and the expression of its coding gene NIA1. Importantly, our analysis utilizing yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) techniques reveals the direct binding of PHR1 to the NIA1 promoter via the P1BS motifs. Subsequent transient transcription expression assay (TTEA) demonstrates PHR1 as a transcriptional suppressor of NIA1. In addition, it was also observed that the SPX (SYG1/Pho81/XPR1) proteins SPX1 and SPX4 can attenuate the transcriptional inhibition of NIA1 by PHR1. Collectively, these findings reveal a mechanism through which PHR1-mediated Pi signal governs N metabolism, thus offering evidence for the precise modulation of plant growth and development via N-P interaction.
Keyword ：
Arabidopsis Arabidopsis NIA1 NIA1 Nitrate reductase Nitrate reductase P1BS motif P1BS motif PHR1 PHR1 Transcriptional regulation Transcriptional regulation
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| GB/T 7714 | Liu, Zhongjuan , Huang, Shaoxuan , Zhu, Lin et al. PHR1 negatively regulates nitrate reductase activity by directly inhibiting the transcription of NIA1 in Arabidopsis [J]. | JOURNAL OF PLANT PHYSIOLOGY , 2024 , 303 . |
| MLA | Liu, Zhongjuan et al. "PHR1 negatively regulates nitrate reductase activity by directly inhibiting the transcription of NIA1 in Arabidopsis" . | JOURNAL OF PLANT PHYSIOLOGY 303 (2024) . |
| APA | Liu, Zhongjuan , Huang, Shaoxuan , Zhu, Lin , Li, Chengquan , Zhang, Duanmei , Chen, Mingxue et al. PHR1 negatively regulates nitrate reductase activity by directly inhibiting the transcription of NIA1 in Arabidopsis . | JOURNAL OF PLANT PHYSIOLOGY , 2024 , 303 . |
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Monovalent cation proton antiporters (CPAs) play crucial roles in ion and pH homeostasis, which is essential for plant development and environmental adaptation, including salt tolerance. Here, 68 CPA genes were identified in soybean, phylogenetically dividing into 11 Na+/H+ exchangers (NHXs), 12 K+ efflux antiporters (KEAs), and 45 cation/H+ exchangers (CHXs). The GmCPA genes are unevenly distributed across the 20 chromosomes and might expand largely due to segmental duplication in soybean. The GmCPA family underwent purifying selection rather than neutral or positive selections. The cis-element analysis and the publicly available transcriptome data indicated that GmCPAs are involved in development and various environmental adaptations, especially for salt tolerance. Based on the RNA-seq data, twelve of the chosen GmCPA genes were confirmed for their differentially expression under salt or osmotic stresses using qRT-PCR. Among them, GmCHX20a was selected due to its high induction under salt stress for the exploration of its biological function on salt responses by ectopic expressing in Arabidopsis. The results suggest that the overexpression of GmCHX20a increases the sensitivity to salt stress by altering the redox system. Overall, this study provides comprehensive insights into the CPA family in soybean and has the potential to supply new candidate genes to develop salt-tolerant soybean varieties.
Keyword ：
cation/proton antiporter cation/proton antiporter GmCHX20a GmCHX20a salt response salt response soybean soybean
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| GB/T 7714 | Jia, Qi , Song, Junliang , Zheng, Chengwen et al. Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (23) . |
| MLA | Jia, Qi et al. "Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 23 (2023) . |
| APA | Jia, Qi , Song, Junliang , Zheng, Chengwen , Fu, Jiahui , Qin, Bin , Zhang, Yongqiang et al. Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (23) . |
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Phosphate (Pi) deficiency is a common stress that limits plant growth and development. Plants exhibit a variety of Pi starvation responses (PSRs), including anthocyanin accumulation. The transcription factors of the PHOSPHATE STARVATION RESPONSE (PHR) family, such as AtPHR1 in Arabidopsis, play central roles in the regulation of Pi starvation signaling. Solanum lycopersicum PHR1-like 1 (SlPHL1) is a recently identified PHR involved in PSR regulation in tomato, but the detailed mechanism of its participation in Pi starvation-inducing anthocyanin accumulation remains unclear. Here we found that overexpression of SlPHL1 in tomato increases the expression of genes associated with anthocyanin biosynthesis, thereby promoting anthocyanin biosynthesis, but silencing SlPHL1 with Virus Induced Gene Silencing (VIGS) attenuated low phosphate (LP) stress-induced anthocyanin accumulation and expression of the biosynthesis-related genes. Notably, SlPHL1 is able to bind the promoters of genes Flavanone 3-Hydroxylase (SlF3H), Flavanone 3 & PRIME;-Hydroxylase (SlF3 & PRIME;H), and Leucoanthocyanidin Dioxygenase (SlLDOX) by yeast one-hybrid (Y1H) analysis. Furthermore, Electrophoretic Mobility Shift Assay (EMSA) and transient transcript expression assay showed that PHR1 binding t (sequence (P1BS) motifs located on the promoters of these three genes are critical for SlPHL1 binding and enhancing the gene transcription. Additionally, allogenic overexpression of SlPHL1 could promote anthocyanin biosynthesis in Arabidopsis under LP conditions through the similar mechanism to AtPHR1, suggesting that SlPHL1 might be functionally conserved with AtPHR1 in this process. Taken together, SlPHL1 positively regulates LP-induced anthocyanin accumulation by directly promoting the transcription of SlF3H, SlF3 & PRIME;H and SlLDOX. These findings will contribute to understanding the molecular mechanism of PSR in tomato.
Keyword ：
Anthocyanin Anthocyanin Low phosphate stress Low phosphate stress PHR1 binding sequence PHR1 binding sequence SlPHL1 SlPHL1 Solanum lycopersicum Solanum lycopersicum
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| GB/T 7714 | Wu, Xueqian , Liu, Zhongjuan , Liu, Yanan et al. SlPHL1 is involved in low phosphate stress promoting anthocyanin biosynthesis by directly upregulation of genes SlF3H, SlF3′H, and SlLDOX in tomato [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2023 , 200 . |
| MLA | Wu, Xueqian et al. "SlPHL1 is involved in low phosphate stress promoting anthocyanin biosynthesis by directly upregulation of genes SlF3H, SlF3′H, and SlLDOX in tomato" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 200 (2023) . |
| APA | Wu, Xueqian , Liu, Zhongjuan , Liu, Yanan , Wang, Enhui , Zhang, Duanmei , Huang, Shaoxuan et al. SlPHL1 is involved in low phosphate stress promoting anthocyanin biosynthesis by directly upregulation of genes SlF3H, SlF3′H, and SlLDOX in tomato . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2023 , 200 . |
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Abstract ：
Phosphate (Pi) deficiency is a common stress that limits plant growth and development. Plants exhibit a variety of Pi starvation responses (PSRs), including anthocyanin accumulation. The transcription factors of the PHOSPHATE RESPONSE (PHR) family, such as AtPHR1 in Arabidopsis, play central roles in the regulation of Pi starvation signaling. Solanum lycopersicum PHR1-like 1 (SlPHL1) is a recently identified PHR involved in PSR regulation in tomato, but the detailed mechanism of its participation in Pi starvation-inducing anthocyanin accumulation remains unclear. Here we found that overexpression of SlPHL1 in tomato increases the expression of genes associated with anthocyanin biosynthesis, thereby promoting anthocyanin biosynthesis, but silencing SlPHL1 with Virus Induced Gene Silencing (VIGS) attenuated low phosphate (LP) stress-induced anthocyanin accumulation and expression of the biosynthesis-related genes. Notably, SlPHL1 can bind the promoters of genes Flavanone 3-Hydroxylase (SlF3H), Flavanone 3'-Hydroxylase (SlF3'H), and Leucoanthocyanidin Dioxygenase (SlLDOX) by yeast one-hybrid (Y1H) analysis. Furthermore, Electrophoretic Mobility Shift Assay (EMSA) and transient transcript expression assay showed that PHR1 binding site (P1BS) motifs located on the promoters of these three genes are critical for SlPHL1 binding and enhancing gene transcription. In addition, overexpression of SlPHL1 in Arabidopsis compensates for impaired LP-induced anthocyanin accumulation caused by AtPHR1 mutation. Moreover, SlPHL1 interacts with the promoters of AtF3'H and AtLDOX via the P1BS motifs and activates their transcription. Taken together, SlPHL1 positively regulates LP-induced anthocyanin accumulation by directly promoting the transcription of SlF3H, SlF3'H and SlLDOX. These findings will contribute to understanding the molecular mechanism of PSR in tomato. © 2023, The Authors. All rights reserved.
Keyword ：
Anthocyanins Anthocyanins Biochemistry Biochemistry Biosynthesis Biosynthesis Electrophoretic mobility Electrophoretic mobility Fruits Fruits Plants (botany) Plants (botany) Transcription Transcription Viruses Viruses
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| GB/T 7714 | Wu, Xueqian , Liu, Zhongjuan , Liu, Yanan et al. SlPHL1 is Involved in Low Phosphate Stress Promoting Anthocyanin Biosynthesis by Directly Upregulation of Genes SLF3h, SLF3'H, and SlLDOX in Tomato [J]. | SSRN , 2023 . |
| MLA | Wu, Xueqian et al. "SlPHL1 is Involved in Low Phosphate Stress Promoting Anthocyanin Biosynthesis by Directly Upregulation of Genes SLF3h, SLF3'H, and SlLDOX in Tomato" . | SSRN (2023) . |
| APA | Wu, Xueqian , Liu, Zhongjuan , Liu, Yanan , Wang, Enhui , Zhang, Duanmei , Huang, Shaoxuan et al. SlPHL1 is Involved in Low Phosphate Stress Promoting Anthocyanin Biosynthesis by Directly Upregulation of Genes SLF3h, SLF3'H, and SlLDOX in Tomato . | SSRN , 2023 . |
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Main conclusion Phosphate deficiency promotes anthocyanin accumulation inArabidopsisthrough direct binding of PHR1 to the P1BS motifs on the promoters ofF3'HandLDOXand thereby upregulating their expression. Phosphorus is one of the essential elements for plants, and plants mainly absorb inorganic phosphate (Pi) from soil. But Pi deficiency is a common factor limiting plant growth and development. Anthocyanin accumulation in green tissues (such as leaves) is one of the characteristics of many plants in response to Pi starvation. However, little is known about the mechanism by which Pi starvation induces anthocyanin accumulation. Here, we found that the mutation of the gene PHOSPHATE STARVATION RESPONSE1 (PHR1), which encodes a key factor involved in Pi starvation signaling in Arabidopsis, significantly attenuates anthocyanin accumulation under Pi-limiting conditions. Moreover, the expression of several Pi deficiency-upregulated genes that are involved in anthocyanin biosyntheses, such as flavanone 3'-hydroxylase (F3'H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX), and production of anthocyanin pigment 1 (PAP1), was significantly lower in the phr1-1 mutant than in the wild type (WT). Both yeast one-hybrid (Y1H) analysis and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) showed that PHR1 can interact with the promoters of F3'H and LDOX, but not DFR and PAP1. By electrophoretic mobility shift assay (EMSA), it was further confirmed that the PHR1-binding sequence (P1BS) motifs located on the F3'H and LDOX promoters are required for the PHR1 bindings. Also, in Arabidopsis protoplasts, PHR1 enhanced the transcriptional activity of the F3'H and LDOX promoters, but these effects were markedly impaired when the P1BS motifs were mutated. Taken together, these results indicate that PHR1 positively regulates Pi starvation-induced anthocyanin accumulation in Arabidopsis, at least in part, by directly binding the P1BS motifs located on the promoters to upregulate the transcription of anthocyanin biosynthetic genes F3'H and LDOX.
Keyword ：
Anthocyanin Anthocyanin Arabidopsis Arabidopsis PHOSPHATE STARVATION RESPONSE1 (PHR1) PHOSPHATE STARVATION RESPONSE1 (PHR1) PHR1-binding sequence PHR1-binding sequence Pi starvation Pi starvation
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| GB/T 7714 | Liu, Zhongjuan , Wu, Xueqian , Wang, Enhui et al. PHR1 positively regulates phosphate starvation-induced anthocyanin accumulation through direct upregulation of genes F3'H and LDOX in Arabidopsis [J]. | PLANTA , 2022 , 256 (2) . |
| MLA | Liu, Zhongjuan et al. "PHR1 positively regulates phosphate starvation-induced anthocyanin accumulation through direct upregulation of genes F3'H and LDOX in Arabidopsis" . | PLANTA 256 . 2 (2022) . |
| APA | Liu, Zhongjuan , Wu, Xueqian , Wang, Enhui , Liu, Yanan , Wang, Yi , Zheng, Qinghua et al. PHR1 positively regulates phosphate starvation-induced anthocyanin accumulation through direct upregulation of genes F3'H and LDOX in Arabidopsis . | PLANTA , 2022 , 256 (2) . |
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Inorganic phosphate (Pi) deficiency is a major limiting factor for plant growth and development. Previous reports have demonstrated that PHOSPHATE STARVATION RESPONSE 1 (PHR1) and OsPHR2 play central roles in Pi-starvation signaling in Arabidopsis and rice, respectively. However, the Pi-starvation signaling network in tomato (Solanum lycopersicum) is still not fully understood. In this work, SlPHL1, a homolog of AtPHR1 and OsPHR2, was identified from tomato. It was found that SlPHL1 contains the MYB and coiled-coil (CC) domains, localizes in the nucleus, and has transcriptional activity, indicating that it is a typical MYB-CC transcription factor (TF). Overexpression of SlPHL1 enhanced Pi-starvation responses both in Arabidopsis Col-0 and in tomato Micro-Tom, including elevated root hair growth, promoted APase activity, favored Pi uptake, and increased transcription of Pi starvation-inducing (PSI) genes. Besides, overexpressing SlPHL1 was able to compensate for the Pi-starvation response weakened by the AtPHR1 mutation. Notably, electrophoretic mobility shift assay (EMSA) showed that SlPHL1 could bind to the PHR1-binding sequence (P1BS, GNATATNC)-containing DNA fragments. Furthermore, SlPHL1 specifically interacted with the promoters of the tomato PSI genes SlPht1;2 and SlPht1;8 through the P1BS cis-elements. Taken these results together, SlPHL1 is a newly identified MYB-CC TF from tomato, which participates in Pi-starvation signaling by directly upregulating the PSI genes. These findings might contribute to the understanding of the Pi-starvation signaling in tomato.
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| GB/T 7714 | Zhang, Yongqiang , Wang, Yi , Wang, Enhui et al. SlPHL1, a MYB-CC transcription factor identified from tomato, positively regulates the phosphate starvation response [J]. | PHYSIOLOGIA PLANTARUM , 2021 , 173 (3) : 1063-1077 . |
| MLA | Zhang, Yongqiang et al. "SlPHL1, a MYB-CC transcription factor identified from tomato, positively regulates the phosphate starvation response" . | PHYSIOLOGIA PLANTARUM 173 . 3 (2021) : 1063-1077 . |
| APA | Zhang, Yongqiang , Wang, Yi , Wang, Enhui , Wu, Xueqian , Zheng, Qinghua , Han, Yizhen et al. SlPHL1, a MYB-CC transcription factor identified from tomato, positively regulates the phosphate starvation response . | PHYSIOLOGIA PLANTARUM , 2021 , 173 (3) , 1063-1077 . |
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Anthocyanin accumulation is a striking symptom of plant environmental response and plays an important role in plant adaptation to adverse stimuli. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) is a member of the PIFs family that directly interacts with light-activated phytochromes, and it can not only regulate various light responses but also optimize growth as a key integrator of multiple signaling pathways. However, the mechanism by which PIF4 participates in the regulation of anthocyanin accumulation remains to be elucidated. In this study, we found that anthocyanin accumulation was effectively induced by white light in Arabidopsis Col-0, but such an effect was impaired in the overexpression line PIF4OX. Consistently, the transcript level of PAP1 that encodes a key transcript factor involved in regulating anthocyanin biosynthesis was significantly decreased in PIF4OX compared with Col-0. Moreover, the expression of PAP1 was markedly lower in pap1-D/PIF4OX than pap1-D, as a result, the phenotype that highly accumulates anthocyanins in leaves of pap1-D caused by PAP1 overexpressing was almost eliminated in pap1-D/PIF4OX. Analyses through chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) and electrophoretic mobility shift assay (EMSA) revealed that PIF4 could directly bind to the G-box motif present in the promoter of PAP1. Furthermore, transient transcriptional expression analysis showed that PIF4 could weaken the transcriptional activity of the PAP1 promoter, and the G-box motif is necessary for the effect of PIF4. Subsequently, when the seedlings shifted from darkness to light and grew under constant red light and short-day photoperiod, it was found that the PAP1 transcription level and anthocyanin content in pif4-2/pap1-D were significantly higher than pap1-D, implying that PIF4 mutation can strengthen PAP1's effect on anthocyanin biosynthesis under these conditions. Taken together, the results indicate that PIF4 negatively regulates anthocyanin accumulation in Arabidopsis through transcriptional suppression of PAP1 by directly binding to the G-box motif of the promoter.
Keyword ：
Anthocyanin Anthocyanin Arabidopsis Arabidopsis PAP1 PAP1 PIF4 PIF4 Transcription Transcription
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| GB/T 7714 | Liu, Zhongjuan , Wang, Yi , Fan, Kai et al. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) negatively regulates anthocyanin accumulation by inhibiting PAP1 transcription in Arabidopsis seedlings [J]. | PLANT SCIENCE , 2021 , 303 . |
| MLA | Liu, Zhongjuan et al. "PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) negatively regulates anthocyanin accumulation by inhibiting PAP1 transcription in Arabidopsis seedlings" . | PLANT SCIENCE 303 (2021) . |
| APA | Liu, Zhongjuan , Wang, Yi , Fan, Kai , Li, Zhaowei , Jia, Qi , Lin, Weiwei et al. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) negatively regulates anthocyanin accumulation by inhibiting PAP1 transcription in Arabidopsis seedlings . | PLANT SCIENCE , 2021 , 303 . |
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Heat shock transcription factors (HSFs) can regulate plant development and stress response. The comprehensive evolutionary history of the HSF family remains elusive in cotton. In this study, each cotton species had 78 members in Gossypium barbadense and Gossypium hirsutum. The diploid species had 39 GaHSFs in Gossypium arboreum, 31 GrHSFs in Gossypium raimondii, 34 GtHSFs in Gossypium turneri, and 34 GlHSFs in Gossypium longicalyx. The HSF family in cotton can be classified into three subfamilies, with seven groups in subfamily A and five groups in subfamily B. Different groups exhibited distinct gene proportions, conserved motifs, gene structures, expansion rates, gene loss rates, and cis-regulatory elements. The paleohexaploidization event led to the expansion of the HSF family in cotton, and the gene duplication events in six Gossypium species were inherited from their common ancestor. The HSF family in diploid species had a divergent evolutionary history, whereas two cultivated tetraploids presented a highly conserved evolution of the HSF family. The HSF members in At and Dt subgenomes of the cultivated tetraploids showed a different evolution from their corresponding diploid donors. Some HSF members were regarded as key candidates for regulating cotton development and stress response. This study provided the comprehensive information on the evolutionary history of the HSF family in cotton.
Keyword ：
Cotton Cotton Expansion Expansion Functional analysis Functional analysis HSF family HSF family Molecular evolution Molecular evolution
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| GB/T 7714 | Fan, Kai , Mao, Zhijun , Ye, Fangting et al. Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species [J]. | GENOMICS , 2021 , 113 (5) : 3112-3127 . |
| MLA | Fan, Kai et al. "Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species" . | GENOMICS 113 . 5 (2021) : 3112-3127 . |
| APA | Fan, Kai , Mao, Zhijun , Ye, Fangting , Pan, Xinfeng , Li, Zhaowei , Lin, Weiwei et al. Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species . | GENOMICS , 2021 , 113 (5) , 3112-3127 . |
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