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学者姓名：罗海凌
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本发明涉及一种云芝新菌种及其栽培方法。所述云芝新菌种为云芝（Trametes versicolor）TvJC209，其是经组织分离纯化、紫外诱变及定向筛选获得，已于2023年6月19日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20231064，保藏地址为中国.武汉.武汉大学该菌种。该菌种的ITS序列与其在NCBI数据库中相似度最高的Trametes versicolor voucher ACAD21091F（OL741530.1）存在1个碱基的差异，A→G；该菌种可以多种干或鲜巨菌草、芦竹等菌草作为培养基主要原料进行栽培；该菌种子实体多糖含量达6％、水溶浸出物含量达22％、三萜含量达0.42％、生物学转化率达20％。该菌种的栽培方法包括母种制备、原种制备、栽培及出芝管理。本发明的有益效果是：可以使用菌草这种绿色有机可持续性的原材料栽培云芝，提供高品质云芝。
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| GB/T 7714 | 林冬梅 , 朱敏 , 张煜隆 et al. 一种云芝新菌种及其菌草栽培方法 : CN202510513082.8[P]. | 2025-04-23 . |
| MLA | 林冬梅 et al. "一种云芝新菌种及其菌草栽培方法" : CN202510513082.8. | 2025-04-23 . |
| APA | 林冬梅 , 朱敏 , 张煜隆 , 李治君 , 谢明君 , 林占熺 et al. 一种云芝新菌种及其菌草栽培方法 : CN202510513082.8. | 2025-04-23 . |
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为探究复方菌草灵芝颗粒的水提工艺及其质量标准，以浸膏得率和浸膏多糖含量为指标，采用单因素和响应面实验考察提取温度、提取时间、料液比、提取次数和浸泡时间对浸膏得率和浸膏多糖含量的影响；参考2015版《中国药典》和国标检测复方菌草灵芝颗粒的粒度、水分含量、干燥失重、装量差异、可溶性、微生物限量和重金属含量；通过薄层色谱法对复方菌草灵芝颗粒中的菌草灵芝、紫苏和富硒茶原材料进行鉴定；通过高效液相色谱法测定复方菌草灵芝颗粒中单糖和茶多酚含量。结果表明：复方菌草灵芝颗粒的最佳水提工艺为提取温度80℃、超声时间2.5 h、料液比(g/mL)1∶25、提取次数2次、浸泡时间6 h;其颗粒粒度、水分含量、干燥失重、装量差异和可溶性均符合2015版《中国药典》要求，微生物限量和重金属含量均符合国标要求，其中硒平均质量分数为0.058 3 mg/kg;得到的菌草灵芝、紫苏和富硒茶的薄层色谱条带清晰，分离效果好，阴性对照对其测定无干扰；复方菌草灵芝颗粒中甘露糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖、表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、儿茶素、没食子酸的质量分数分别为1.310、1.166、8.708、1.265、0.824、5.79、2.58、1.72和1.67 mg/g。以上结果表明优化得到的复方菌草灵芝颗粒水提工艺稳定可靠，颗粒的各项指标均符合相关要求，建立的复方菌草灵芝颗粒水提工艺质量标准科学可行。
Keyword ：
水提工艺 水提工艺 菌草灵芝 菌草灵芝 薄层色谱 薄层色谱 质量标准 质量标准 高效液相色谱 高效液相色谱
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| GB/T 7714 | 张桂清 , 梁志豪 , 刘青 et al. 复方菌草灵芝颗粒的水提工艺及其质量标准研究 [J]. | 陕西师范大学学报(自然科学版) , 2025 , 53 (03) : 66-82 . |
| MLA | 张桂清 et al. "复方菌草灵芝颗粒的水提工艺及其质量标准研究" . | 陕西师范大学学报(自然科学版) 53 . 03 (2025) : 66-82 . |
| APA | 张桂清 , 梁志豪 , 刘青 , 吴小平 , 罗海凌 , 姚俊新 et al. 复方菌草灵芝颗粒的水提工艺及其质量标准研究 . | 陕西师范大学学报(自然科学版) , 2025 , 53 (03) , 66-82 . |
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本发明涉及一种低温型血红栓菌新菌种TsJC206及其菌草栽培方法。TsJC206其可用一种或多种菌草作为培养基主要原料，如巨菌草、芦竹等，甚至可用巨菌草鲜草、芦竹鲜草等栽培，子实体三萜含量达1.15％、多糖含量达10.82％、生物学转化率达25％；二是为低温型菌种，出芝温度15~20℃，显著低于现有技术报道的出芝温度，补充了血红栓菌的出芝温型；三是其NCBI数据库中ITS序列第182位点的碱基为G，与其在NCBI数据库中相似度最高的OR062414.1在第223位点的碱基A存在差异。所述栽培方法包括母种制备、原种制备、栽培及出芝管理。本发明有益效果：可在15~20℃较低温条件下出芝；可用菌草这种绿色有机可持续性的原材料栽培血红栓菌；可用鲜菌草代替部分培养料，减少干燥培养料的步骤，降低成本。
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| GB/T 7714 | 林冬梅 , 张煜隆 , 谢明君 et al. 一种低温型血红栓菌新菌种及其菌草栽培方法 : CN202510514332.X[P]. | 2025-04-23 . |
| MLA | 林冬梅 et al. "一种低温型血红栓菌新菌种及其菌草栽培方法" : CN202510514332.X. | 2025-04-23 . |
| APA | 林冬梅 , 张煜隆 , 谢明君 , 林占熺 , 李治君 , 罗海凌 et al. 一种低温型血红栓菌新菌种及其菌草栽培方法 : CN202510514332.X. | 2025-04-23 . |
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本发明涉及一种热带灵芝新菌种及其栽培方法。所述热带灵芝新菌种为热带灵芝（Ganoderma tropicum）GtJC215，在中国典型培养物保藏中心的保藏编号为CCTCC NO：M 20221411。其特点在于：在NCBI数据库中ITS序列第69和155位点的碱基为C和A，与其在NCBI数据库中相似度最高的Ganoderma tropicum voucher Yuan3490（JQ781880.1）菌种在第109和195位点的碱基A和G存在差异；可用多种草本植物作为培养基主要原料；可在夏季高温高湿时正常出芝，转化率可达25％，三萜和多糖含量分别为1.48％和1.12％，达国家标准。所述热带灵芝新菌种的栽培方法包括母种制备、原种制备、栽培及出芝管理。本发明的有益效果是：可以使用菌草这种绿色有机可持续性的原材料栽培热带灵芝；可在夏季高温赤芝不宜出芝的时期出产灵芝。
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| GB/T 7714 | 林冬梅 , 张煜隆 , 谢明君 et al. 一种热带灵芝新菌种GtJC215及其栽培方法 : CN202510513786.5[P]. | 2025-04-23 . |
| MLA | 林冬梅 et al. "一种热带灵芝新菌种GtJC215及其栽培方法" : CN202510513786.5. | 2025-04-23 . |
| APA | 林冬梅 , 张煜隆 , 谢明君 , 李治君 , 林占熺 , 林兴生 et al. 一种热带灵芝新菌种GtJC215及其栽培方法 : CN202510513786.5. | 2025-04-23 . |
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Soil salinization and alkalization are serious global challenges that adversely affect crop growth and yield. In this study, six genotypes of giant reed (Arundo donax) seedlings (LvZhou_No.1, LvZhou_No.3, LvZhou_No.6, LvZhou_No.11, LvZhou_No.12 and LvZhou_Var.) originating from different regions of China and Rwanda were utilized as experimental materials. This study aimed to investigate the physiological and biochemical responses of various genotypes to saline-alkali stress and to identify stress-tolerant resources. A mixture saline-alkali solution with a molar ratio of NaCl: Na2SO4: NaHCO3: Na2CO3 = 1:1:1:1 was prepared at three concentrations (75, 150 and 225 millimolar (mM)) for a 7-day pot experiment. Growth and physiological indices were measured at the seedling stage, and salt tolerance was evaluated accordingly. The results indicated the following: the growth indices were significantly reduced across seedlings of all genotypes when the concentration of stress exceeded 150 mM (p < 0.05). There was no significant difference in chlorophyll content (SPAD value) and maximum photochemical efficiency of PS II (F-v/F-m) with increasing saline-alkali stress. However, the photosynthetic rate (P-n), stomatal conductance (G(s)) and transpiration rate (T-r) exhibited decreasing trends, reaching their lowest levels at 225 mM. In contrast, the intercellular CO2 concentration (C-i) value decreased to its lowest at 150 mM but increased at 225 mM. Relative electrical conductivity (REC) and the contents of malondialdehyde (MDA), proline (Pro) and soluble sugar (SS) increased progressively with higher stress concentrations. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly enhanced at stress concentrations above 150 mM. The saline-alkali tolerance of A. donax seedlings was comprehensively evaluated using principal component analysis and membership function analysis based on 15 parameters. The results indicate that P-n, T-r and G(s) are effective physiological indicators for assessing saline-alkali tolerance of A. donax seedlings. The six genotypes were ranked for saline-alkali tolerance as follows: LZ_No.1 > LZ_No.11 > LZ_No.12 > LZ_Var. > LZ_No.3 > LZ_No.6. This indicates that LZ_No.1 shows the highest resistance to saline-alkali stress, whereas LZ_No.6 is the most severely affected, classifying it as a salinity-sensitive genotype. In conclusion, LZ_No.1 exhibits robust saline-alkali tolerance and represents a valuable germplasm resource for improving saline-alkali tolerance in A. donax propagation. The results not only support the development of resilient plants for saline-alkali environments but also offer insights into the mechanisms of salinity tolerance.
Keyword ：
Arundo donax Arundo donax correlation analysis correlation analysis physiological and biochemical responses physiological and biochemical responses saline-alkali stress saline-alkali stress saline-alkali tolerance evaluation saline-alkali tolerance evaluation
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| GB/T 7714 | Cai, Yangxing , Cao, Xiuming , Liu, Bin et al. Saline-Alkali Tolerance Evaluation of Giant Reed (Arundo donax) Genotypes Under Saline-Alkali Stress at Seedling Stage [J]. | AGRONOMY-BASEL , 2025 , 15 (2) . |
| MLA | Cai, Yangxing et al. "Saline-Alkali Tolerance Evaluation of Giant Reed (Arundo donax) Genotypes Under Saline-Alkali Stress at Seedling Stage" . | AGRONOMY-BASEL 15 . 2 (2025) . |
| APA | Cai, Yangxing , Cao, Xiuming , Liu, Bin , Lin, Hui , Luo, Hailing , Liu, Fengshan et al. Saline-Alkali Tolerance Evaluation of Giant Reed (Arundo donax) Genotypes Under Saline-Alkali Stress at Seedling Stage . | AGRONOMY-BASEL , 2025 , 15 (2) . |
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为提高菌草栽培中食用菌菌糠的循环利用效率，以莱牧1号幼苗为试验材料，设在土壤中添加不同质量的平菇菌糠基质，分别为CK(0 g·kg
Keyword ：
生长性状 生长性状 莱牧1号 莱牧1号 菌糠基质 菌糠基质 菌草平菇 菌草平菇
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| GB/T 7714 | 罗海凌 . 土壤中添加菌草平菇菌糠基质对莱牧1号幼苗生长的影响 [J]. | 福建农业科技 , 2025 , 56 (01) : 44-47 . |
| MLA | 罗海凌 . "土壤中添加菌草平菇菌糠基质对莱牧1号幼苗生长的影响" . | 福建农业科技 56 . 01 (2025) : 44-47 . |
| APA | 罗海凌 . 土壤中添加菌草平菇菌糠基质对莱牧1号幼苗生长的影响 . | 福建农业科技 , 2025 , 56 (01) , 44-47 . |
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Long-term plant residue retention can effectively replenish soil quality and fertility. In this study, we collected rhizosphere soil from the residual roots of annual Cenchrus fungigraminus in the Ulan Buh Desert over the past 10 years. The area, depth, and length of these roots decreased over time. The cellulose content of the residual roots was significantly higher in the later 5 years (2018-2022) than the former 5 years (2013-2017), reaching its highest value in 2021. The lignin content of the residual roots did not differ across samples except in 2015 and reached its highest level in 2021. The total sugar of the residual roots in 2022 was 227.88 +/- 30.69 mg & sdot;g(-1), which was significantly higher than that in other years. Compared to the original sandy soil, the soil organic matter and soil microbial biomass carbon (SMBC) contents were 2.17-2.41 times and 31.52-35.58% higher in the later 3 years (2020-2022) and reached the highest values in 2020. The residual roots also significantly enhanced the soil carbon stocks from 2018-2022. Soil dehydrogenase, nitrogenase, and N-acetyl-beta-D-glucosidase (S-NAG) were significantly affected from 2019-2022. The rhizosphere soil community richness and diversity of the bacterial and fungal communities significantly decreased with the duration of the residual roots in the sandy soil, and there was a significant difference for 10 years. Streptomyces, Bacillus, and Sphigomonas were the representative bacteria in the residual root rhizosphere soil, while Agaricales and Panaeolus were the enriched fungal genera. The distance-based redundancy analysis and partial least square path model results showed that the duration of residual roots in the sandy soil, S-NAG, and SMBC were the primary environmental characteristics that shaped the microbial community. These insights provide new ideas on how to foster the exploration of the use of annual herbaceous plants for sandy soil improvement in the future.
Keyword ：
Cenchrus fungigraminus Cenchrus fungigraminus long-term long-term residual root residual root soil microbial community soil microbial community soil quality soil quality
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| GB/T 7714 | Li, Jing , Zhang, Lili , Yu, Shikui et al. Long-Term Benefits of Cenchrus fungigraminus Residual Roots Improved the Quality and Microbial Diversity of Rhizosphere Sandy Soil through Cellulose Degradation in the Ulan Buh Desert, Northwest China [J]. | PLANTS-BASEL , 2024 , 13 (5) . |
| MLA | Li, Jing et al. "Long-Term Benefits of Cenchrus fungigraminus Residual Roots Improved the Quality and Microbial Diversity of Rhizosphere Sandy Soil through Cellulose Degradation in the Ulan Buh Desert, Northwest China" . | PLANTS-BASEL 13 . 5 (2024) . |
| APA | Li, Jing , Zhang, Lili , Yu, Shikui , Luo, Zongzhi , Su, Dewei , Zheng, Dan et al. Long-Term Benefits of Cenchrus fungigraminus Residual Roots Improved the Quality and Microbial Diversity of Rhizosphere Sandy Soil through Cellulose Degradation in the Ulan Buh Desert, Northwest China . | PLANTS-BASEL , 2024 , 13 (5) . |
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【目的】筛选腋芽萌发期耐盐性较强的芦竹属菌草品种，为该类菌草耐盐品种培育提供参考。【方法】以国内外不同地区的12个芦竹属菌草品种为试材，对150 mmol·L~(-1) NaCl溶液处理后腋芽萌发期的各个指标(芽长、根长、芽鲜质量、根鲜质量、发芽势、发芽率、发芽指数和活力指数)进行测定。利用模糊隶属函数法和聚类分析法对供试品种的耐盐性进行综合评价。【结果】与对照组相比，在150 mmol·L~(-1) NaCl胁迫下，所有供试品种的发芽势均降低，芽长、根长、芽鲜质量和根鲜质量都减小；除LZ＿NO.1、LZ＿NO.3和LZ＿NO.7外，其他品种的发芽率均下降；活力指数受盐胁迫的影响最大。基于各指标耐盐系数隶属函数值的临近度对12个品种进行聚类分析，结果表明：LZ＿NO.1、LZ＿NO.3和LZ＿NO.7为一类，属高耐盐型；LZ＿NO.2、LZ＿NO.4和LZ＿NO.6为一类，属盐敏感型。【结论】腋芽萌发期耐盐性较强的芦竹属菌草品种为LZ＿NO.1、LZ＿NO.3和LZ＿NO.7;根据活力指数可判定芦竹属菌草品种的质量优劣。
Keyword ：
耐盐性 耐盐性 聚类分析 聚类分析 腋芽萌发 腋芽萌发 芦竹属菌草 芦竹属菌草 隶属函数分析法 隶属函数分析法
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| GB/T 7714 | 蔡杨星 , 林冬梅 , 曹秀明 et al. 芦竹属菌草腋芽萌发期耐盐性评价 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (04) : 541-548 . |
| MLA | 蔡杨星 et al. "芦竹属菌草腋芽萌发期耐盐性评价" . | 福建农林大学学报(自然科学版) 53 . 04 (2024) : 541-548 . |
| APA | 蔡杨星 , 林冬梅 , 曹秀明 , 刘斌 , 林辉 , 罗海凌 et al. 芦竹属菌草腋芽萌发期耐盐性评价 . | 福建农林大学学报(自然科学版) , 2024 , 53 (04) , 541-548 . |
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本发明属于生物技术领域，具体涉及芦竹属菌草抗寒关键基因Adona0G1018300及其应用。所述芦竹属菌草抗寒关键基因Adona0G1018300的核苷酸序列如SEQ ID NO.1所示，其编码的氨基酸序列如SEQ ID NO.2所示。本发明通过构建过表达载体将芦竹属菌草抗寒关键基因Adona0G1018300转入模式植物拟南芥中，分别对野生型（WT）和转Adona0G1018300基因拟南芥进行低温胁迫试验，然后测定其超氧化物歧化酶活性、过氧化物酶活性、丙二醛含量、脯氨酸含量、叶绿素含量，综合这5个指标进行主成分、相关性、线性组合分析。结果显示，转Adona0G1018300基因拟南芥的抗寒性大于WT。表明芦竹属菌草Adona0G1018300基因可以提高拟南芥的抗寒性，是抗寒关键基因。
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| GB/T 7714 | 林辉 , 林冬梅 , 罗海凌 et al. 芦竹属菌草抗寒关键基因Adona0G1018300及其应用 : CN202410816935.0[P]. | 2024-06-24 . |
| MLA | 林辉 et al. "芦竹属菌草抗寒关键基因Adona0G1018300及其应用" : CN202410816935.0. | 2024-06-24 . |
| APA | 林辉 , 林冬梅 , 罗海凌 , 邵恩斯 , 李玖荣 . 芦竹属菌草抗寒关键基因Adona0G1018300及其应用 : CN202410816935.0. | 2024-06-24 . |
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为评价鲜菌草工厂化周年瓶栽银耳的质量和安全性，以木屑、棉籽壳原料为对照，利用‘绿洲1号’鲜菌草为主要原料工厂化周年瓶栽银耳，测定不同配方栽培组银耳子实体蛋白质、氨基酸、总糖、粗多糖、多糖肽等营养成分的含量及重金属含量、农药残留、棉酚含量，分析其质量和安全性.结果显示：‘绿洲1号’鲜菌草配方栽培组(F1)工厂化栽培的银耳，其蛋白质含量分别比木屑配方栽培组(CK1)、棉籽壳配方栽培组(CK2)高8.7%、53.2%,且差异显著；F1的总糖含量与CK1相同；F1的氨基酸总量分别比CK1、CK2高6.7%、46.1%,且差异显著；在棉籽壳栽培料中加入鲜菌草可提高银耳子实体蛋白质和氨基酸的含量；F1的粗多糖含量分别比CK1、CK2高5.0%、6.8%;F1的重金属含量、农药残留符合绿色食品标准，且不含棉酚.综上，用‘绿洲1号’鲜菌草工厂化瓶栽可提高银耳的质量和安全性.
Keyword ：
工厂化周年栽培 工厂化周年栽培 质量和安全 质量和安全 银耳 银耳 鲜菌草 鲜菌草
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| GB/T 7714 | 林兴生 , 林占熺 , 林冬梅 et al. 鲜菌草工厂化周年瓶栽银耳质量和安全性评价 [J]. | 福建农林大学学报(自然科学版) , 2023 , 52 (01) : 65-70 . |
| MLA | 林兴生 et al. "鲜菌草工厂化周年瓶栽银耳质量和安全性评价" . | 福建农林大学学报(自然科学版) 52 . 01 (2023) : 65-70 . |
| APA | 林兴生 , 林占熺 , 林冬梅 , 林辉 , 罗海凌 , 王泽辉 et al. 鲜菌草工厂化周年瓶栽银耳质量和安全性评价 . | 福建农林大学学报(自然科学版) , 2023 , 52 (01) , 65-70 . |
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