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学者姓名：郑华坤
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Abstract ：
The vacuole degrades and recycles endocytic and autophagic cargos, while the retromer complex sorts cargos from the endosomes to the trans-Golgi network or the plasma membrane, thus preventing unnecessary vacuolar degradation. However, whether the retromer complex regulates vacuolar proteolytic system during autophagic substrate degradation remains unclear. This study demonstrates that the retromer complex regulates both general and selective autophagy by ensuring the delivery of vacuolar protease(s) into the vacuole lumen in the rice blast fungus Magnaporthe oryzae. The central retromer subunit, MoVps35, transports the serine protease MoPrb1 from the endosomes to the vacuole lumen. Deletion of MoVPS35 or any other retromer component prevents the transport of MoPrb1-GFP into the vacuole lumen. Consistently, Delta Moprb1 mutant shows similar defects as the retromer mutants, including failure of autophagy-dependent conidiation and plant infection. Additionally, mutation of the catalytic residues of MoPrb1 (Asp 192, His 224 and Ser 390) reduces autophagy flux. Furthermore, MoVps35 also interacts with another aspartyl protease MoPep4 via MoPrb1. Loss of MoPEP4 leads to abnormal micro-autophagy (pexophagy) but not to fungal development and pathogenicity. Overall, this study demonstrates a crucial role of the retromer complex in the regulation of macro- and micro-autophagy by different vacuolar proteases in M. oryzae.
Keyword ：
macro-autophagy macro-autophagy Magnaporthe oryzae Magnaporthe oryzae pexophagy pexophagy retromer complex retromer complex vacuolar proteases vacuolar proteases
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| GB/T 7714 | Zhang, Dingyang , Hu, Jiexiong , Hong, Yonghe et al. Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus [J]. | ADVANCED SCIENCE , 2025 , 12 (41) . |
| MLA | Zhang, Dingyang et al. "Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus" . | ADVANCED SCIENCE 12 . 41 (2025) . |
| APA | Zhang, Dingyang , Hu, Jiexiong , Hong, Yonghe , Fan, Yuping , Peng, Minghui , Abubakar, Yakubu Saddeeq et al. Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus . | ADVANCED SCIENCE , 2025 , 12 (41) . |
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Magnaporthe oryzae (syn. Pyricularia oryzae), the causative agent of devastating crop diseases, exhibits remarkable genomic plasticity that contributes to its adaptability and pathogenicity. Individual M. oryzae strains may contain supernumerary mini-chromosomes, which are dispensable and highly repetitive. Here, we explored the stability of two mini-chromosomes of a Lolium strain isolated in the US, TF05-1, in which one mini-chromosome contains sequences nearly identical to the mini-chromosome of the wheat isolate B71 from Bolivia. The discordance of their phylogenetic relationships based on genomic polymorphisms in core chromosomes and polymorphisms in mini-chromosomes indicated horizontal transfer of the mini-chromosome. Contour-clamped homogeneous electric field (CHEF) karyotyping and genome sequencing analysis found variation in numbers and sizes of mini-chromosomes among asexual monoconidial progeny of TF05-1. Optimization of a fluorescence in situ hybridization (FISH) protocol enabled single-cell karyotyping and revelation of drastic cellular variation in numbers of mini-chromosomes. In addition, rearrangement within mini-chromosomes occurred frequently in the TF05-1 progeny. We characterized an intrachromosomal rearrangement presumably mediated by a palindrome repeat. The rearrangement resulted in a 300-kb deletion and a 900-kb duplication. We found that, in contrast to Long Terminal Repeat (LTR) retrotransposons in core chromosomes, LTR retrotransposons in mini-chromosomes were more recently inserted, less methylated, and with higher G + C content. The data indicated that most LTR retrotransposons in mini-chromosomes retain high activity and have yet to be silenced by fungal genome defense mechanisms such as repeat-induced point mutation, which may contribute to highly dynamic mini-chromosome content in fungi.
Keyword ：
Blast disease Blast disease Genome assembly Genome assembly Genomic dynamic Genomic dynamic Magnaporthe oryzae Magnaporthe oryzae mini-chromosome mini-chromosome Supernumerary chromosome Supernumerary chromosome
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| GB/T 7714 | Lin, Guifang , Zheng, Huakun , Koo, Dal-Hoe et al. Highly dynamic supernumerary mini-chromosomes in a Magnaporthe oryzae strain contributes to cellular variance of genomic content [J]. | FUNGAL GENETICS AND BIOLOGY , 2025 , 180 . |
| MLA | Lin, Guifang et al. "Highly dynamic supernumerary mini-chromosomes in a Magnaporthe oryzae strain contributes to cellular variance of genomic content" . | FUNGAL GENETICS AND BIOLOGY 180 (2025) . |
| APA | Lin, Guifang , Zheng, Huakun , Koo, Dal-Hoe , Wang, Zonghua , Cook, David , Valent, Barbara et al. Highly dynamic supernumerary mini-chromosomes in a Magnaporthe oryzae strain contributes to cellular variance of genomic content . | FUNGAL GENETICS AND BIOLOGY , 2025 , 180 . |
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Many pathogenic fungi display 'two-speed genome', with the fast-evolving genomic compartments enriched with repetitive sequences, particularly the transposons, which have been shown to drive the variation of pathogenicity-associated genes. Supernumerary chromosomes (SCs) are known to facilitate genomic variation in fungal pathogens, but their specific role in such processes remains understudied. In this study, we assessed the transferability of SCs between asexual Magnaporthe oryzae strains during co-culture and co-infection, and investigated their role in genome reconstruction through experimental evolution assays. We found that SCs could be horizontally transferred between M. oryzae strains and revealed frequent structural variations facilitated by SCs, including deletions, duplications, translocations, and SC-core chromosome recombinations during and after horizontal transfer. Remarkably, all observed intra- and inter-chromosome rearrangements were confined to core chromosome ends and SCs, indicating a robust role of SCs in facilitating genetic exchange within fast-evolving genomic compartments. Additionally, SC carrying the avirulence gene AvrPikE modulates M. oryzae virulence against Pikh rice through horizontal transfer, loss of whole SC, and segmental deletions. Our findings establish SCs as critical players in shaping the diversity and dynamics of the pathogenic fungal genomes, highlighting them as a cradle for the variation of pathogenicity-associated genes. (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)"(sic)(sic)(sic)(sic)(sic)"(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)((sic)(sic)(sic)(sic)(sic)(sic))(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic)(sic)(Supernumerary chromosomes, SCs)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic). (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(Magnaporthe oryzae)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)SCs(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)SCs(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic). (sic)(sic)(sic)(sic)SCs(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)SCs(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic),(sic)(sic)(sic)(sic)SC(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)SCs(sic), (sic)(sic)SCs(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)AvrPikE(sic)SC(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)Pikh(sic)(sic)(sic)(sic)(sic)(sic). (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), SCs(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)"(sic)(sic)"(sic)(sic)(sic)(sic)(sic).
Keyword ：
experimental evolution experimental evolution horizontal chromosome transfer horizontal chromosome transfer Magnaporthe oryzae Magnaporthe oryzae pathogenicity pathogenicity supernumerary chromosome supernumerary chromosome
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| GB/T 7714 | Fang, Zhenyu , Li, Yuyong , Huang, Jianqiang et al. Experimental insights into genome reconstruction driven by horizontal transfer of supernumerary chromosomes in Magnaporthe oryzae [J]. | NEW PHYTOLOGIST , 2025 , 248 (1) : 140-156 . |
| MLA | Fang, Zhenyu et al. "Experimental insights into genome reconstruction driven by horizontal transfer of supernumerary chromosomes in Magnaporthe oryzae" . | NEW PHYTOLOGIST 248 . 1 (2025) : 140-156 . |
| APA | Fang, Zhenyu , Li, Yuyong , Huang, Jianqiang , Chen, Meilian , Chen, Xiali , Mo, Xueting et al. Experimental insights into genome reconstruction driven by horizontal transfer of supernumerary chromosomes in Magnaporthe oryzae . | NEW PHYTOLOGIST , 2025 , 248 (1) , 140-156 . |
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The fungal pathogens exhibit diverse genome architecture, which facilitates the host adaptation. Although increasing high-quality genomic data enable insights into the genome architecture of many fungal pathogens during the last decades, genomic features of many fungal species are still not fully characterized. Here, we identified a Pyriculariaceae family fungal strain Xenopyricularia zizaniicola JB-1 causing the leaf spot disease on Zizania latifolia and revealed its distinct genome compartment features. The fungal strain JB-1 was identified as X. zizaniicola based on the Koch's postulate, conidial morphology, and phylogenetic analysis. Using 2.51 Gb PacBio HiFi sequencing data, the JB-1 genome was assembled into nine contigs, five of which contain telomeric repeats at both ends. The genome size is 40,888,459 bp with an N50 of 6,431,016 bp, and a total of 9,894 protein-coding genes were predicted. BUSCO assessment demonstrated high completeness, with 754 (99.47%) of the 758 BUSCO orthologs identified as complete. The absence of both repeat-rich regions at chromosome ends and preferential residing of pathogenicity-associated genes (PAGs) in the repeat-rich regions indicated a genome compartment dissimilar to the "two-speed genome" commonly observed in Pyricularia oryzae, indicating a distinct evolution drive of the PAGs in X. zizaniicola strain JB-1. Additionally, the JB-1 genome encodes fewer PAGs compared to other members of family Pyriculariaceae. These findings provide valuable genomic resources of family Pyriculariaceae and will facilitate future studies on host-pathogen interactions and the development of effective disease management strategies for X. zizaniicola.IMPORTANCEThe family Pyriculariaceae includes notorious pathogens that annually result in significant agricultural losses. The genome architecture of plant fungal pathogens reflects their evolutionary adaptation to host-pathogen interactions. However, limited knowledge exists regarding the genomic features of other species within family Pyriculariaceae, particularly those associated with the economically important crop Zizania latifolia. In this study, we assembled the first high-quality genome of Xenopyricularia zizaniicola strain JB-1, which infects Z. latifolia, and revealed its distinct genome architecture. We provide evidence that the distribution pattern of pathogenicity-associated genes in X. zizaniicola strain JB-1 closely resembles the "one-speed genome" structure, which contrasts with Pyricularia oryzae. Our findings provide valuable resources for genomic studies within family Pyriculariaceae and contribute to our understanding of the adaptive evolution of pathogens to their hosts.
Keyword ：
genome assembly genome assembly one-speed genome one-speed genome pathogenicity-associated genes pathogenicity-associated genes Xenopyricularia zizaniicola Xenopyricularia zizaniicola Zizania latifolia Zizania latifolia
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| GB/T 7714 | Fang, Zhenyu , Li, Yuyong , Huang, Jianqiang et al. The Xenopyricularia zizaniicola exhibits a genome architecture distinct to the two-speed genome [J]. | MICROBIOLOGY SPECTRUM , 2025 , 13 (7) . |
| MLA | Fang, Zhenyu et al. "The Xenopyricularia zizaniicola exhibits a genome architecture distinct to the two-speed genome" . | MICROBIOLOGY SPECTRUM 13 . 7 (2025) . |
| APA | Fang, Zhenyu , Li, Yuyong , Huang, Jianqiang , Wang, Jianhong , Lian, Xiwen , Lin, Shuhui et al. The Xenopyricularia zizaniicola exhibits a genome architecture distinct to the two-speed genome . | MICROBIOLOGY SPECTRUM , 2025 , 13 (7) . |
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RNA demethylation plays an important role in diverse biological processes. Intriguingly, RNA demethylation has not been reported in bamboo, which is known for its rapid growth. PheALKBH9, an m6A demethylase in bamboo, was stably transformed into rice and increased its susceptibility to rice blast disease. Heterologous expression of PheALKBH9 reduced the overall m6A modification levels in rice. Using HyperTRIBE (Targets of RNA-binding proteins Identified By Editing), we identified evolutionarily conserved PheALKBH9 target RNAs in both rice and Moso bamboo. Overexpression of PheALKBH9 led to higher protein expression and shorter poly(A) tails. Notably, PheALKBH9 directly bound to CCR4-associated factor1 (CAF1G) and poly(A)-binding genes (PABPC1 and PABPC2), potentially modulating poly(A) tail lengths. In addition, PheALKBH9 also bound to and removed m6A modifications from Perox4, JAZ7, and METS2, key players in plant immunity, suggesting that PheALKBH9 plays a role in plant disease resistance. In summary, our study unveils a previously unknown role of PheALKBH9-mediated m6A demethylation in response to blast disease and provides insights into its mechanisms in monocotyledonous plants. PheALKBH9, an m6A demethylase from Moso bamboo, regulates plant responses to biotic and abiotic stresses by modulating m6A on target RNAs and shortening poly(A) tails.
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| GB/T 7714 | Wang, Huihui , Wang, Huiyuan , Jia, Yue et al. HyperTRIBE mapping of the RNA m6A demethylase ALKBH9 binding sites in bamboo reveals its role in plant defense [J]. | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| MLA | Wang, Huihui et al. "HyperTRIBE mapping of the RNA m6A demethylase ALKBH9 binding sites in bamboo reveals its role in plant defense" . | PLANT PHYSIOLOGY 199 . 2 (2025) . |
| APA | Wang, Huihui , Wang, Huiyuan , Jia, Yue , Li, Tuhe , Yang, Siyu , Jin, Yandong et al. HyperTRIBE mapping of the RNA m6A demethylase ALKBH9 binding sites in bamboo reveals its role in plant defense . | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
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The genomes of the fungus Magnaporthe oryzae that causes blast diseases on diverse grass species, including major crops, have indispensable core-chromosomes and may contain supernumerary chromosomes, also known as mini-chromosomes. These mini-chromosomes are speculated to provide effector gene mobility, and may transfer between strains. To understand the biology of mini-chromosomes, it is valuable to be able to detect whether a M. oryzae strain possesses a mini-chromosome. Here, we applied recurrent neural network models for classifying DNA sequences as arising from core- or mini-chromosomes. The models were trained with sequences from available core- and mini-chromosome assemblies, and then used to predict the presence of mini-chromosomes in a global collection of M. oryzae isolates using short-read DNA sequences. The model predicted that mini-chromosomes were prevalent in M. oryzae isolates. Interestingly, at least one mini-chromosome was present in all recent wheat isolates, but no mini-chromosomes were found in early isolates collected before 1991, indicating a preferential selection for strains carrying mini-chromosomes in recent years. The model was also used to identify assembled contigs derived from mini-chromosomes. In summary, our study has developed a reliable method for categorizing DNA sequences and showcases an application of recurrent neural networks in predictive genomics.
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| GB/T 7714 | Gyawali, Nikesh , Hao, Yangfan , Lin, Guifang et al. Using recurrent neural networks to detect supernumerary chromosomes in fungal strains causing blast diseases [J]. | NAR GENOMICS AND BIOINFORMATICS , 2024 , 6 (3) . |
| MLA | Gyawali, Nikesh et al. "Using recurrent neural networks to detect supernumerary chromosomes in fungal strains causing blast diseases" . | NAR GENOMICS AND BIOINFORMATICS 6 . 3 (2024) . |
| APA | Gyawali, Nikesh , Hao, Yangfan , Lin, Guifang , Huang, Jun , Bika, Ravi , Daza, Lidia Calderon et al. Using recurrent neural networks to detect supernumerary chromosomes in fungal strains causing blast diseases . | NAR GENOMICS AND BIOINFORMATICS , 2024 , 6 (3) . |
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Endoplasmic reticulum (ER) is an enclosed three-dimensional eukaryotic membrane network composed of flattened sacs. Fusion of homologous membranes to the ER membrane is essential for the maintenance of this network structure. In yeast, ER membrane fusion is mediated by Sey1p, whose paralogues function distinctly in different species. In this study, we investigated the biological functions of MoSEY1 in the devastating rice blast fungus Magnaporthe oryzae by functional genomic approach. Compared to wild type, deletion of MoSEY1 considerably decreased the growth and conidia production of M. oryzae. Additionally, the absence of MoSEY1 delayed appressorium formation and invasive hyphae growth. The appressorium function was also impaired in Delta Mosey1 mutant. Subcellular localization analysis revealed that MoSey1 is localized at the ER. The Delta Mosey1 mutant showed augmented sensitivity to ER stress. Additionally, we found that MoSey1 regulated the unfolded protein response, autophagy, and protein secretion in M. oryzae. In conclusion, our study unveiled the involvement of MoSey1 in the development, pathogenesis, and ER functions in M. oryzae.
Keyword ：
Endoplasmic reticulum Endoplasmic reticulum Magnaporthe oryzae Magnaporthe oryzae MoSey1 MoSey1 Pathogenicity Pathogenicity Protein secretion Protein secretion
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| GB/T 7714 | Yang, Zifeng , Li, Meiqin , Huang, Linwan et al. MoSey1 regulates the unfolded protein response, appressorium development, and pathogenicity of Magnaporthe oryzae [J]. | PHYTOPATHOLOGY RESEARCH , 2024 , 6 (1) . |
| MLA | Yang, Zifeng et al. "MoSey1 regulates the unfolded protein response, appressorium development, and pathogenicity of Magnaporthe oryzae" . | PHYTOPATHOLOGY RESEARCH 6 . 1 (2024) . |
| APA | Yang, Zifeng , Li, Meiqin , Huang, Linwan , Chen, Xinru , Weng, Shuning , Jules, Biregeya et al. MoSey1 regulates the unfolded protein response, appressorium development, and pathogenicity of Magnaporthe oryzae . | PHYTOPATHOLOGY RESEARCH , 2024 , 6 (1) . |
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Fungi from the Pyricularia genus cause blast disease in many economically important crops and grasses, such as wheat, rice, and Cenchrus grass JUJUNCAO. Structure variation associated with the gain and loss of effectors contributes largely to the adaptive evolution of this fungus towards diverse host plants. A telomere-to-telomere genome assembly would facilitate the identification of genome-wide structural variations through comparative genomics. Here, we report a telomere-to-telomere, near-complete genome assembly of a Pyricularia penniseti isolate JC-1 infecting JUJUNCAO. The assembly consists of eight core chromosomes and two supernumerary chromosomes, named mini1 and mini2, spanning 42.1 Mb. We annotated 12,156 protein-coding genes and identified 4.54% of the genome as repetitive sequences. The two supernumerary chromosomes contained fewer genes and more repetitive sequences than the core chromosomes. Our genome and results provide valuable resources for the future study in genome evolution, structure variation and host adaptation of the Pyricularia fungus.
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| GB/T 7714 | Li, Yuyong , Wang, Xianjun , Huang, Jianqiang et al. Near complete assembly of Pyricularia penniseti infecting Cenchrus grass identified its eight core chromosomes [J]. | SCIENTIFIC DATA , 2024 , 11 (1) . |
| MLA | Li, Yuyong et al. "Near complete assembly of Pyricularia penniseti infecting Cenchrus grass identified its eight core chromosomes" . | SCIENTIFIC DATA 11 . 1 (2024) . |
| APA | Li, Yuyong , Wang, Xianjun , Huang, Jianqiang , Fang, Zhenyu , Lian, Xiwen , Lu, Guodong et al. Near complete assembly of Pyricularia penniseti infecting Cenchrus grass identified its eight core chromosomes . | SCIENTIFIC DATA , 2024 , 11 (1) . |
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Dynamic transposition of transposable elements (TEs) in fungal pathogens has significant impact on genome stability, gene expression, and virulence to the host. In Magnaporthe oryzae, genome plasticity resulting from TE insertion is a major driving force leading to the rapid evolution and diversification of this fungus. Despite their importance in M. oryzae population evolution and divergence, our understanding of TEs in this context remains limited. Here, we conducted a genome-wide analysis of TE transposition dynamics in the 11 most abundant TE families in M. oryzae populations. Our results show that these TEs have specifically expanded in recently isolated M. oryzae rice populations, with the presence/absence polymorphism of TE insertions highly concordant with population divergence on Geng/Japonica and Xian/Indica rice cultivars. Notably, the genes targeted by clade-specific TEs showed clade-specific expression patterns and are involved in the pathogenic process, suggesting a transcriptional regulation of TEs on targeted genes. Our study provides a comprehensive analysis of TEs in M. oryzae populations and demonstrates a crucial role of recent TE bursts in adaptive evolution and diversification of the M. oryzae rice-infecting lineage. IMPORTANCE Magnaporthe oryzae is the causal agent of the destructive blast disease, which caused massive loss of yield annually worldwide. The fungus diverged into distinct clades during adaptation toward the two rice subspecies, Xian/Indica and Geng/Japonica. Although the role of TEs in the adaptive evolution was well established, mechanisms underlying how TEs promote the population divergence of M. oryzae remain largely unknown. In this study, we reported that TEs shape the population divergence of M. oryzae by differentially regulating gene expression between Xian/Indica-infecting and Geng/Japonica-infecting populations. Our results revealed a TE insertion-mediated gene expression adaption that led to the divergence of M. oryzae population infecting different rice subspecies.
Keyword ：
population divergence population divergence rice blast disease rice blast disease rice subspecies adaptation rice subspecies adaptation transposable element transposable element
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| GB/T 7714 | Lin, Lianyu , Sun, Ting , Guo, Jiayuan et al. Transposable elements impact the population divergence of rice blast fungus Magnaporthe oryzae [J]. | MBIO , 2024 , 15 (5) . |
| MLA | Lin, Lianyu et al. "Transposable elements impact the population divergence of rice blast fungus Magnaporthe oryzae" . | MBIO 15 . 5 (2024) . |
| APA | Lin, Lianyu , Sun, Ting , Guo, Jiayuan , Lin, Lili , Chen, Meilian , Wang, Zhe et al. Transposable elements impact the population divergence of rice blast fungus Magnaporthe oryzae . | MBIO , 2024 , 15 (5) . |
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Magnaporthe oryzae, a filamentous heterothallic ascomycete fungus that serves as the causative agent of rice blast disease, is globally distributed in rice-growing regions. Populations shaped by environmental factors and human intervention play important roles in the formation of genetic structure. In this study, population structures and spatiotemporal dynamics were investigated based on large-scale whole genomic sequences of rice-infecting M. oryzae around the world. By analyzing these genetic structures, we identified divergent clades that crossed geographic boundaries. While we observed associations between the isolates and their geographic origins, we also found that there were frequent migration events occurring across Asia in main rice cultivation regions. Within Asia, China was the migration origin, facilitating gene flows to Japan and South Korea. Since the 1970s, the genetic diversity of M. oryzae populations in China has also shown a steadily increasing trend, continuing through to the 2020s. Additionally, our analysis of the evolutionary history of Asian M. oryzae populations provided insights into the population expansion that has taken place in recent decades. Overall, our findings indicate that human-mediated gene flows played a pivotal role in shaping the genetic structure of M. oryzae.
Keyword ：
gene flow gene flow genetic diversity genetic diversity Magnaporthe oryzae Magnaporthe oryzae population structure population structure
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| GB/T 7714 | Duan, Guohua , Liu, Yuchan , Zheng, Cheng et al. Chinese Populations of Magnaporthe oryzae Serving as a Source of Human-Mediated Gene Flow to Asian Countries: A Population Genomic Analysis [J]. | JOURNAL OF FUNGI , 2024 , 10 (11) . |
| MLA | Duan, Guohua et al. "Chinese Populations of Magnaporthe oryzae Serving as a Source of Human-Mediated Gene Flow to Asian Countries: A Population Genomic Analysis" . | JOURNAL OF FUNGI 10 . 11 (2024) . |
| APA | Duan, Guohua , Liu, Yuchan , Zheng, Cheng , Yu, Kaihui , Xie, Jiahui , Wang, Baohua et al. Chinese Populations of Magnaporthe oryzae Serving as a Source of Human-Mediated Gene Flow to Asian Countries: A Population Genomic Analysis . | JOURNAL OF FUNGI , 2024 , 10 (11) . |
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