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学者姓名:刘先
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为探究低强度计划火烧后亚热带森林土壤细菌群落的恢复特征及其对火烧黑炭输入的响应,本研究以亚热带马尾松人工林火烧迹地为对象,设置不同火后黑炭输入量处理(移除C0、单倍C1和双倍C2)和未火烧对照(UB),分别于火后1年和5年采集0~10 cm土壤样品,利用高通量测序技术分析土壤细菌群落多样性和结构特征,并结合土壤因子探讨其调控机制。结果发现,计划火烧和黑炭处理对土壤细菌群落多样性无显著影响(P>0.05)。与火后1年相比,火后5年土壤细菌群落扩增子序列变体(ASV)数目和Chao1指数显著降低(P<0.01),Shannon指数显著增加(P<0.05)。基于Bray-Curtis距离的NMDS分析表明,火后1年土壤移除黑炭土壤细菌群落结构与对照土壤差异显著(P<0.05),而火烧5年后不同处理之间土壤细菌群落结构相似(P>0.05)。土壤pH是土壤细菌群落结构的关键调控因子,分别能解释火后1年和5年土壤细菌群落结构变化的46.3%和50.5%。酸杆菌门、变形菌门、放线菌门和绿弯菌门是土壤中的优势细菌类群。与火后1年土壤相比,火后5年土壤变形菌门和放线菌门相对多度显著降低,C0和C1土壤厚壁菌门细菌相对多度显著增加(P<0.05)。C1土壤芽单胞菌门相对多度显著高于UB土壤;火后5年,C0和C1土壤厚壁菌门相对多度显著高于对照UB土壤,C0土壤厚壁菌门相对多度显著高于C2土壤(P<0.05)。综上,亚热带森林土壤细菌群落多样性和结构能在低强度火后短期内恢复到未火烧水平,火烧黑炭对土壤细菌类群相对多度具有长期调控效应,未来应结合土壤养分循环等生态功能持续关注亚热带森林火后土壤关键细菌类群的动态变化。
Keyword :
土壤细菌 土壤细菌 多样性 多样性 炼山 炼山 群落结构 群落结构 黑炭 黑炭
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| GB/T 7714 | 满家瑞 , 王款 , 焦梓轩 et al. 马尾松采伐迹地火后土壤细菌群落恢复特征 [J]. | 生态学杂志 , 2025 , 44 (04) : 1125-1134 . |
| MLA | 满家瑞 et al. "马尾松采伐迹地火后土壤细菌群落恢复特征" . | 生态学杂志 44 . 04 (2025) : 1125-1134 . |
| APA | 满家瑞 , 王款 , 焦梓轩 , 焦鹏宇 , 严强 , 刘先 et al. 马尾松采伐迹地火后土壤细菌群落恢复特征 . | 生态学杂志 , 2025 , 44 (04) , 1125-1134 . |
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Background and AimsAge-related alterations in nitrogen (N) acquisition by canopy trees has been extensively explored; however, little is known regarding N uptake strategy of coexisting plant species during forest plantation development. MethodsA field experiment was conducted to evaluate plant N uptake in subtropical Cunninghamia lanceolata plantations of varying ages (7, 15, 24, and 34 years). Using a hydroponic method, we assessed the root capacity to uptake NH4+, NO3-, and glycine by the coexisting tree (C. lanceolata), a shrub (Ficus hirta), and a herb (Pteris semipinnata). ResultsBoth the canopy and understory vegetation preferred NH4+ as a N source in hydroponic culture, followed by glycine and then NO3- . Root N uptake rates with increasing plantation age varied by plant species and N source. For F. hirta, the NO3- uptake rate increased from 0.38 to 0.78 mu g N.g(-1) root.h(-1), while it decreased from 1.64 to 0.30 mu g N.g(-1) root.h(-1) for P. semipinnata as the C. lanceolata plantation developed. No clear pattern was observed for C. lanceolata. A positive relationship between root AM colonization rate and NO3- uptake rate was observed for F. hirta, but not for C. lanceolata and P. semipinnata. The contribution of glycine to P. semipinnata total N uptake increased progressively with plantation age. ConclusionOur results indicate that N acquisition among coexisting tree and understory vegetation varied with plant species and N form in hydroponic culture. Notably, understory plants exhibited greater flexibility in N acquisition than overstory trees during the development of subtropical C. lanceolata plantations.
Keyword :
Arbuscular mycorrhizal colonization Arbuscular mycorrhizal colonization Cunninghamia lanceolate Cunninghamia lanceolate N-15 isotope labeling N-15 isotope labeling Nitrogen uptake Nitrogen uptake Stand age Stand age
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| GB/T 7714 | Ren, Mengfan , Zhang, Qiuxia , Deluca, Thomas H. et al. Coexisting understory vegetation exhibits greater plasticity than canopy trees in nitrogen acquisition in Chinese fir plantations [J]. | PLANT AND SOIL , 2025 . |
| MLA | Ren, Mengfan et al. "Coexisting understory vegetation exhibits greater plasticity than canopy trees in nitrogen acquisition in Chinese fir plantations" . | PLANT AND SOIL (2025) . |
| APA | Ren, Mengfan , Zhang, Qiuxia , Deluca, Thomas H. , Wei, Lili , Liu, Xian , Hu, Yalin et al. Coexisting understory vegetation exhibits greater plasticity than canopy trees in nitrogen acquisition in Chinese fir plantations . | PLANT AND SOIL , 2025 . |
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Biochar is produced by burning biomass under oxygen- limited conditions, and it has been widely used as a soil amendment to improve soil functions such as nutri-ent retention. However, whether the impact of biochar application on soil nitro-gen (N) transformation and N2O emission varies with the pyrolysis temperature remains unclear, especially in different forest types in subtropical regions. In this study, a 60- day laboratory incubation experiment was conducted to evaluate the impact of biochar with different pyrolysis temperatures (300 degrees C [BC300], 500 degrees C [BC500], and 800 degrees C [BC800]) on net N transformation rates and N2O emission in soils collected from Castanopsis kawakamii dominated natural forest (NF) and Chinese fir (Cunninghamia lanceolate, CF) plantation in subtropical China. The results showed that the application of biochar significantly increased soil ammo-nium (NH4+) content (p< 0.001) but reduced nitrate (NO3-) content (p< 0.001) compared with the control. The soil NH4+ content of the BC800 treatment was significantly higher than that of other treatments (p< 0.001). Biochar application significantly reduced soil net N mineralization (NRmin) and nitrification (NRnit) rate (p< 0.001), but increased net ammonification (NRamm) rate (p< 0.001). The application of biochar led to a remarkable decrease in cumulative N2O emission com-pared to the control (p< 0.001). In particular, soils treated with high- temperature biochar emitted significantly lower N2O compared to other treatments (p< 0.001). The partial least squares path model demonstrated that biochar influenced N2O emission through a direct effect in NF soil and an indirect effect in CF soil. This study highlights the distinct role of biochar, particularly that produced under high pyrolysis temperatures as a soil amendment to mitigate N2O emission and promote N retention in both subtropical natural and planted forests.
Keyword :
biochar biochar Cunninghamia lanceolate Cunninghamia lanceolate N2O emission N2O emission nitrogen retention nitrogen retention nitrogen transformation nitrogen transformation pyrolysis temperature pyrolysis temperature
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| GB/T 7714 | Cheng, Liutao , Wang, Bingtao , Ren, Mengfan et al. Biochar produced at high temperature mitigates N2O emission and promotes nitrogen retention in subtropical forest soils [J]. | GLOBAL CHANGE BIOLOGY BIOENERGY , 2024 , 16 (3) . |
| MLA | Cheng, Liutao et al. "Biochar produced at high temperature mitigates N2O emission and promotes nitrogen retention in subtropical forest soils" . | GLOBAL CHANGE BIOLOGY BIOENERGY 16 . 3 (2024) . |
| APA | Cheng, Liutao , Wang, Bingtao , Ren, Mengfan , Wang, Yuzhe , Hu, Yalin , Liu, Xian . Biochar produced at high temperature mitigates N2O emission and promotes nitrogen retention in subtropical forest soils . | GLOBAL CHANGE BIOLOGY BIOENERGY , 2024 , 16 (3) . |
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Charcoal, a byproduct of biomass burning, is widely and heterogeneously distributed in fire-affected ecosystems. However, few field studies have been conducted to evaluate the effects of fire-deposited charcoal on the post-fire soil quality. In this study, we aimed to investigate whether charcoal generated during post timber harvest broadcast burning influenced the recovery of individual soil properties and overall soil quality in subtropical forest plantations. Broadcast burning was conducted on experimental Pinus massoniana plantation timber harvest sites in southern China. Surface soils (0-10 cm) were collected in plots established on the burnt sites five years after the disturbance event. Plots were established immediately after burning with three different levels of charcoal input (C0, removal of all visible charcoal; C1, charcoal retained in-situ; C2, charcoal removed from C0 added to C2) and an unburnt control (UB). Thirty-two soil indicators representing soil physical, chemical, and biological properties were determined. The results showed that 11 indicators exhibited significant differences among the treatments, with significantly lower SOM, microbial biomass carbon (MBC), peroxidase (POD) and leucine aminopeptidase (LAP) activities for the C0 and C1 plots than the UB plots (P < 0.05), while no significant difference was observed between the UB and C2 plot (P > 0.05). The soil available N, P and exchangeable nutrient contents were not significantly different among the treatments (P > 0.05). Our results indicate that SOM and microbial attributes did not recover five years after the disturbance event and that charcoal appeared to play a positive role in the post-fire soil restoration. Whether the accelerated recovery of soil restoration induced by charcoal might contribute to higher forest productivity needs to be testified in combination with plant growth and performance analysis in the future.
Keyword :
Broadcast burning Broadcast burning Charcoal manipulation Charcoal manipulation Minimum data set Minimum data set Soil quality indicators Soil quality indicators Soil recovery Soil recovery
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| GB/T 7714 | Jiao, Pengyu , DeLuca, Thomas H. , Wang, Kuan et al. Fire-deposited charcoal enhances soil microbial biomass in a recently harvested subtropical plantation forest [J]. | GEODERMA , 2024 , 448 . |
| MLA | Jiao, Pengyu et al. "Fire-deposited charcoal enhances soil microbial biomass in a recently harvested subtropical plantation forest" . | GEODERMA 448 (2024) . |
| APA | Jiao, Pengyu , DeLuca, Thomas H. , Wang, Kuan , Liu, Xian , Hu, Yalin , Wang, Yuzhe . Fire-deposited charcoal enhances soil microbial biomass in a recently harvested subtropical plantation forest . | GEODERMA , 2024 , 448 . |
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Charcoal, a byproduct resulting from incomplete combustion of biomass in fire events, can modify the physical properties of soil due to its high porosity and large surface area. To evaluate the impact of fire-deposited charcoal on soil hydraulic characteristics, soil-charcoal mixtures were analyzed to investigate the effects of different application doses (wt%: 0, 1%, 3%, 5%, 10% and 20%) of charcoal on soil bulk density (BD), porosity (total, capillary, and non-capillary), residual moisture after free drainage (RM), saturated water content (SC), and saturated hydraulic conductivity (Ks) of loamy and sandy soils collected from subtropical forests in south China. The results showed that the impact of charcoal on soil's physical and hydraulic properties depends on the soil type and the application dose. The incorporation of charcoal significantly decreased the BD of sandy soil (p < 0.001), while a significant decrease in BD in loamy soil was only observed as a result of the higher application doses (10% and 20%) (p < 0.001). Charcoal application doses of 5% or higher led to a significant increase in the total porosity (TP) of sandy soil (p < 0.001) and doses of 3% and 20% resulted in a significant increase in the TP of loamy soil (p < 0.001). The capillary porosity (CP) of both sand and loamy soils significantly increased when charcoal was applied at doses of 3% or higher (p < 0.001). The minimum charcoal application dose that significantly increased the RM in sandy soil was 5%, while for loamy soil, the minimum effective dose was 10%. Charcoal applied at a dose of 3% significantly increased the Ks of sandy soil (p < 0.001), while no significant effect on Ks was observed for loamy soil (p > 0.05). Collectively, our findings suggest that fire-derived charcoal enhances the soil water-retention capacity in subtropical forests, with the effects becoming more pronounced at higher application doses and being particularly notable in sandy soil compared to loamy soil.
Keyword :
charcoal charcoal loamy soil loamy soil sandy soil sandy soil saturated hydraulic conductivity saturated hydraulic conductivity water retention water retention
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| GB/T 7714 | Cheng, Liutao , Wang, Kuan , Yao, Zhi et al. Naturally Deposited Charcoal Enhances Water Retention Capacity of Subtropical Forest Soils [J]. | FORESTS , 2024 , 15 (11) . |
| MLA | Cheng, Liutao et al. "Naturally Deposited Charcoal Enhances Water Retention Capacity of Subtropical Forest Soils" . | FORESTS 15 . 11 (2024) . |
| APA | Cheng, Liutao , Wang, Kuan , Yao, Zhi , Liu, Xian , Zhao, Dan , Wang, Yuzhe . Naturally Deposited Charcoal Enhances Water Retention Capacity of Subtropical Forest Soils . | FORESTS , 2024 , 15 (11) . |
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随着社会对于专业复合型人才要求的日益提升和研究生招生规模的进一步扩大,近年来硕士研究生的报考率和录取率呈逐年增加态势。同时,对硕士研究生的创新和科学思维的能力要求越来越高,在这种背景下,培养具有科研能力、创新能力和终身学习能力的人才成为当代研究生教育的首要目标。通过对福建农林大学研究生“英文论文写作与学术报告”课程的教学改革实践,探索了如何在课堂学习过程中培养研究生的学术意识、科学思维与思辨能力。
Keyword :
农林类高校 农林类高校 学术报告 学术报告 研究生 研究生 科学思维能力 科学思维能力 英文学术论文写作 英文学术论文写作
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| GB/T 7714 | 刘先 . 研究生英文论文写作与科学思维能力培养——福建农林大学林学院硕士研究生的教改实践 [J]. | 教育教学论坛 , 2023 , 4 (31) : 33-36 . |
| MLA | 刘先 . "研究生英文论文写作与科学思维能力培养——福建农林大学林学院硕士研究生的教改实践" . | 教育教学论坛 4 . 31 (2023) : 33-36 . |
| APA | 刘先 . 研究生英文论文写作与科学思维能力培养——福建农林大学林学院硕士研究生的教改实践 . | 教育教学论坛 , 2023 , 4 (31) , 33-36 . |
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随着社会对于专业复合型人才要求的日益提升和研究生招生规模的进一步扩大,近年来硕士研究生的报考率和录取率呈逐年增加态势.同时,对硕士研究生的创新和科学思维的能力要求越来越高,在这种背景下,培养具有科研能力、创新能力和终身学习能力的人才成为当代研究生教育的首要目标.通过对福建农林大学研究生"英文论文写作与学术报告"课程的教学改革实践,探索了如何在课堂学习过程中培养研究生的学术意识、科学思维与思辨能力.
Keyword :
农林类高校 农林类高校 学术报告 学术报告 研究生 研究生 科学思维能力 科学思维能力 英文学术论文写作 英文学术论文写作
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| GB/T 7714 | 刘先 . 研究生英文论文写作与科学思维能力培养 [J]. | 教育教学论坛 , 2023 , (31) : 33-36 . |
| MLA | 刘先 . "研究生英文论文写作与科学思维能力培养" . | 教育教学论坛 31 (2023) : 33-36 . |
| APA | 刘先 . 研究生英文论文写作与科学思维能力培养 . | 教育教学论坛 , 2023 , (31) , 33-36 . |
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PurposeTannin is the fourth most abundant biochemical compound in vascular plants. Due to its protein-binding capacity, tannin can interfere with soil nitrogen (N) biogeochemical cycling processes and potentially contribute to soil N conservation. Yet little is known about the effect and mechanism of tannin on regulating soil N transformation process.Materials and methodsWe devised microcosm study to evaluate the effects of different concentration and types of tannin on N-cycling processes. Soils were collected from subtropical Chinese fir plantation and incubated for 28 days. Two incubation experiments were carried out simultaneously; one was treated with different concentrations of condensed tannin (CT), including low (1 mg center dot g-1), middle (5 mg center dot g-1), and high (10 mg center dot g-1) concentrations of tannin, while the other was treated with different types of tannin including tannin acid (TA), catechin (CA), and tannin acid + catechin (TC). Soil enzyme activities, MBC, MBN, DOC, DON, soil N transformation rate and respiration were measured in both experiments.Results and discussionIn both incubation experiments, tannin amendment significantly lowered soil nitrate content compared with control soil, as well as soil net nitrification and mineralization rates. In contrast, we observed consistent increase of soil enzyme activities involved in N cycling (amidase, N-acetyl-beta-D-glocosaminidase, polyphenol oxidase, peroxidase, urease and laccase) under tannin treatments. This stimulating effect on soil enzyme activities is likely to due to the fact that tannin may serve as a carbon substrate for soil microorganisms rather than a toxic compound, as indicated by enhancing soil respiration with tannin addition. However, the stimulating effect on soil respiration eclipsed as the incubation proceeded. Furthermore, soil net mineralization rate, on average, decreased by 7.8%, 38.2%, and 69.1%, respectively, under low, middle, and high concentrations of tannin-added soils compared with control.ConclusionsThis study highlights the distinct role of tannin with different concentrations and types in regulating soil N cycling in a subtropical forest. Addition of tannin generally increased soil enzyme activities and soil respiration rate, whereas, the effects of different tannin type on soil N mineralization and nitrification remain largely unpredictable.
Keyword :
Condensed tannin Condensed tannin Soil enzyme activities Soil enzyme activities Soil net mineralization Soil net mineralization Subtropical forest Subtropical forest
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| GB/T 7714 | Liu, Xian , Yao, Zhi , Cheng, Liutao et al. Condensed tannin addition decreased soil nitrate but increased soil enzyme activities in subtropical forest soil [J]. | JOURNAL OF SOILS AND SEDIMENTS , 2023 , 24 (2) : 537-551 . |
| MLA | Liu, Xian et al. "Condensed tannin addition decreased soil nitrate but increased soil enzyme activities in subtropical forest soil" . | JOURNAL OF SOILS AND SEDIMENTS 24 . 2 (2023) : 537-551 . |
| APA | Liu, Xian , Yao, Zhi , Cheng, Liutao , Gao, Ziyue , Ren, Lihong , Wang, Yuzhe et al. Condensed tannin addition decreased soil nitrate but increased soil enzyme activities in subtropical forest soil . | JOURNAL OF SOILS AND SEDIMENTS , 2023 , 24 (2) , 537-551 . |
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黑炭是不完全燃烧生成的具有高度芳香化结构的含碳颗粒物,能对火烧迹地土壤生态功能产生长期影响.以中亚热带马尾松人工林采伐火烧迹地为研究对象,分析火后黑炭处理[移除(B_0)、单倍(B_1)、双倍(B_2)]、未火烧对照(UB)5 a后土壤有机碳组分[可溶性有机碳(DOC)、微生物量碳(MBC)、易氧化有机碳(ROC)、惰性有机碳(RC)、粗颗粒有机碳(CPOC)、细颗粒有机碳(FPOC)]含量和碳转化酶[葡萄糖苷酶(BG)、过氧化物酶(POD)、多酚氧化酶(POX)、纤维素酶(CBH)、蔗糖酶(SC)]活性特征.结果表明,B_1处理土壤DOC、 MBC、 CPOC、 FPOC和RC含量均显著低于UB(P<0.05).B_2处理土壤MBC和FPOC含量与UB无显著差异,但显著高于B_0处理(P<0.001).B_1和B_2处理土壤MBC/TC与UB无显著差异,而B_0处理土壤MBC/TC显著低于UB(P<0.05).B_0、 B_1和B_2处理土壤BG和POD活性显著低于UB(P<0.01).B_0和B_2处理土壤POX活性显著低于对照(P<0.01).不同黑炭处理土壤TC、 DOC、 ROC、 CPOC和RC含量以及碳转化酶活性无显著差异(P>0.05).冗余分析(RDA)结果显示,SC和POX活性为土壤有机碳组分含量的主要影响因子,其解释率分别为16.3%和12.7%.结果表明,火烧产生的黑炭能加快火后MBC恢复、促进土壤有机碳累积和提高土壤有机碳稳定性,今后在中亚热带人工林经营管理中应重视对黑炭的利用.
Keyword :
土壤微生物量碳 土壤微生物量碳 有机碳组分 有机碳组分 炼山 炼山 酶活性 酶活性 黑炭 黑炭
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| GB/T 7714 | 姚智 , 焦鹏宇 , 吴晓生 et al. 马尾松采伐迹地火烧黑炭对土壤有机碳组分和碳转化酶活性的影响 [J]. | 环境科学 , 2023 , 44 (07) : 4201-4210 . |
| MLA | 姚智 et al. "马尾松采伐迹地火烧黑炭对土壤有机碳组分和碳转化酶活性的影响" . | 环境科学 44 . 07 (2023) : 4201-4210 . |
| APA | 姚智 , 焦鹏宇 , 吴晓生 , 严强 , 刘先 , 胡亚林 et al. 马尾松采伐迹地火烧黑炭对土壤有机碳组分和碳转化酶活性的影响 . | 环境科学 , 2023 , 44 (07) , 4201-4210 . |
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研究不同连栽代数杉木人工林土壤氮循环功能基因丰度的变化特征及其调控因子,可为亚热带人工林定向调控土壤氮素转化过程,提高氮素利用效率并减少其负面效应提供科学依据.以中亚热带不同连栽代数(一代FRP、二代SRP、三代TRP)杉木人工林和格氏栲天然林(NF)为研究对象,采用荧光定量PCR技术测定冬季和夏季土壤细菌16S rRNA、真菌18S rRNA和参与氮循环功能基因AOA、AOB、nifH、nirK、nirS、nosZ丰度,并分析其与土壤理化因子的相关关系.结果显示,天然林转变为杉木人工林后,整体上各氮循环功能基因丰度在天然林与一代林之间无显著差异.不同连栽代数杉木林土壤AOA和AOB基因丰度在两季变化规律一致,AOA基因丰度随连栽代数增加呈显著递减趋势(P<0.05),AOB基因丰度无显著差异.连栽杉木林土壤16S rRNA、18S rRNA、nifH、nirK、nirS和nosZ基因丰度变化特征因季节而异,冬季土壤nifH(7.05-8.15 copies/g)、nirS(6.78-8.08 copies/g)和nos Z(7.39-8.08 copies/g)基因丰度随连栽代数增加而下降,夏季土壤nirK(8.26-9.26 copies/g)基因丰度随连栽代数增加则为先增加后下降;杉木林土壤细菌16S(10.36-11.03 copies/g)基因丰度高于真菌18S(7.34-8.20 copies/g)基因丰度,且二者均为夏季略高于冬季.土壤含水量、可溶性有机氮和硝态氮可以较大程度地解释土壤功能基因丰度的变化.本研究表明亚热带杉木人工林中土壤AOA相比于AOB在数量上更占据优势地位,且其丰度随着连栽代数增加呈下降趋势,说明硝化作用可能随连栽代数增加而减弱,这也许是土壤系统在氮素匮乏条件下所采取的一种保氮策略.(图2表4参44)
Keyword :
功能基因丰度 功能基因丰度 杉木人工林 杉木人工林 氮循环 氮循环 连栽 连栽
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| GB/T 7714 | 唐楚珺 , 高李文 , 彭紫薇 et al. 连栽杉木人工林土壤氮循环功能基因丰度变化 [J]. | 应用与环境生物学报 , 2023 , 29 (01) : 154-162 . |
| MLA | 唐楚珺 et al. "连栽杉木人工林土壤氮循环功能基因丰度变化" . | 应用与环境生物学报 29 . 01 (2023) : 154-162 . |
| APA | 唐楚珺 , 高李文 , 彭紫薇 , 刘雨晖 , 陈辉 , 王玉哲 et al. 连栽杉木人工林土壤氮循环功能基因丰度变化 . | 应用与环境生物学报 , 2023 , 29 (01) , 154-162 . |
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