Query：
学者姓名：林文雄
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
Rice straw return is a crucial practice for recycling organic matter in agricultural systems, but long-term incorporation can lead to recalcitrant carbon (C) accumulation, suppress nutrient release, and inhibit microbial metabolism, thereby reducing soil nutrient availability and crop productivity. Lime and decomposing agents are common supplementary measures, yet their rhizosphere microbiome-mediated mechanisms remain poorly understood. Here, we conducted a field study on a long-term tobacco-rice rotation straw return platform in Southern China (established 2003) during 2020-2021, including straw removal (RM), conventional straw return (SR), and ecological enhancement treatments (SR + L: lime; SR + A: decomposing agent). We evaluated crop productivity, soil nutrient availability, and microbial nutrient acquisition, and profiled rhizosphere microbial responses during the tobacco season using high-throughput sequencing. RM and SR showed no significant difference in yields, whereas SR suppressed soil C and nitrogen (N) availability and reduced tobacco dry matter and N accumulation. In contrast, SR + L and SR + A enhanced rhizosphere nutrient acquisition: SR + L increased Nacquiring enzyme activities by 29.6-35.7 %, and SR + A increased C-acquiring activities by 35.3-49.4 %. Both treatments improved soil carbon and nitrogen availability in the tobacco season by 5.6-8.4 % and 1.5-1.7 %, and in the rice season by 4.5-5.8 % and 1.6-1.9 %, while also enhancing phosphorus (P) availability, alleviating microbial carbon and nitrogen limitation, and increasing tobacco and rice yields by 16.5 % and 4.2 %, respectively. Under SR, key rhizosphere microbial communities exhibited inhibited tricarboxylic acid cycle, glycolysis, and N and P transformation pathways, explaining reduced nutrient availability and crop productivity. Ecological enhancement activated metabolic pathways, optimized fungal communities, promoted saprotrophic fungi, and suppressed pathogens, achieving multi-pathway, multi-element functional integration that improved soil ecological function and system stability, while identifying the core species Acidothermus, which was significantly positively correlated with nutrient availability. These findings reveal the coupled mechanisms of nutrient limitation and microbial functional impairment under long-term straw return and highlight the pivotal roles of keystone microbial communities and core species in ecological restoration and yield improvement, providing a theoretical basis and practical guidance for optimized straw return management and precise ecological interventions.
Keyword ：
Crop rotation pattern Crop rotation pattern Microbial function Microbial function Nutrient availability Nutrient availability Straw return Straw return
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Bianhong , Li, Rikun , Chen, Zhicheng et al. Optimizing soil microbial functions under long-term straw return enhances nutrient acquisition, thereby improving nutrient availability and crop productivity in tobacco-rice rotation systems of southern China [J]. | AGRICULTURE ECOSYSTEMS & ENVIRONMENT , 2026 , 397 . |
| MLA | Zhang, Bianhong et al. "Optimizing soil microbial functions under long-term straw return enhances nutrient acquisition, thereby improving nutrient availability and crop productivity in tobacco-rice rotation systems of southern China" . | AGRICULTURE ECOSYSTEMS & ENVIRONMENT 397 (2026) . |
| APA | Zhang, Bianhong , Li, Rikun , Chen, Zhicheng , Zeng, Chunli , Liao, Songquan , Liu, Yazhou et al. Optimizing soil microbial functions under long-term straw return enhances nutrient acquisition, thereby improving nutrient availability and crop productivity in tobacco-rice rotation systems of southern China . | AGRICULTURE ECOSYSTEMS & ENVIRONMENT , 2026 , 397 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Context: Ratoon rice relies on stubble axillary buds for secondary harvesting, but mechanical harvesting damages stubbles, while improper water and nitrogen management exacerbates nitrogen loss and yield instability. Objective: To alleviate rolling damage and improve the efficiency of bud-promoting fertilizer (BPF) in machineharvested low stubble ratoon rice (MHLR), crop management strategies were explored through yield formation and nitrogen loss assessments. Methods: A two-year, three-factor split-split plot experiment was conducted. Main plots were soil drying degree (Heavy drying, HD; Light drying, LD), subplots were main crop nitrogen management (Postponed nitrogen application; Traditional nitrogen), and sub-subplots were BPF rates (0, 45, 90 kg N ha-1). Results: Compared to traditional nitrogen application, postponed nitrogen application boosted root surface area (RSA) by 6.43 % and post - anthesis dry matter by 9.1 %, and increased main crop yield by 3.59 %. Postponed nitrogen application lessened dependence on BPF. Under PNN0 (HD, postponed nitrogen, 0 kg N ha-1 BPF), stubble quality and ratoon ability in the non - rolling zone did not decline significantly compared to NN90 (HD, traditional nitrogen, 90 kg N ha-1 BPF). Postponed nitrogen application enhanced the non - rolling zone' s ratoon yield by 6.18 %. In wet grain-filling years, HD improved the rolling zone' s ratoon crop yield by 36.31 %. BPF increased ratoon crop yield, but higher rates provided no further significant gains and even significantly increased total nitrogen losses. Notably, PNN0 achieved an annual yield comparable to that of NN90. Compared with NN90, PNN0 significantly reduced annual cumulative ammonia volatilization (31.11 %), N2O emissions (35.01 %), and nitrogen leaching loss (21.46 %). Results from the 1 5N fertilizer tracing indicated that, in comparison to NN90, PNN0 significantly enhanced the proportion of 1 5N fertilizer accumulated in plants by 21.20% and reduced the proportion of 1 5N fertilizer loss by 25.92%. Conclusion: Within MHLR systems, postponed nitrogen application combined with heavy soil drying and no BPF stabilized annual yield while minimizing nitrogen losses.
Keyword ：
Mechanized harvesting Mechanized harvesting Nitrogen losses Nitrogen losses Ratoon rice Ratoon rice Yield Yield
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lan, Chaojie , Xu, Hailong , Qin, Bin et al. Optimizing water and nitrogen management to balance yield and nitrogen loss in mechanically harvested low-stubble ratoon rice [J]. | FIELD CROPS RESEARCH , 2026 , 336 . |
| MLA | Lan, Chaojie et al. "Optimizing water and nitrogen management to balance yield and nitrogen loss in mechanically harvested low-stubble ratoon rice" . | FIELD CROPS RESEARCH 336 (2026) . |
| APA | Lan, Chaojie , Xu, Hailong , Qin, Bin , Li, Jinying , Zhang, Bianhong , Zou, Jingnan et al. Optimizing water and nitrogen management to balance yield and nitrogen loss in mechanically harvested low-stubble ratoon rice . | FIELD CROPS RESEARCH , 2026 , 336 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The root is one of the most important organs that determines the final grain yield in rice. Auxin is essential for root development in plants. Rice auxin response factor7 (OsARF7), belonging to the ARF family, is a key regulator of root development. Here, we show that OsARF7 positively regulates root development via auxin signaling. The osarf7 mutants display a significant decrease in the root number, adventitious root (AR) number and length, and primary root (PR) length, compared with the wild-type. Exogenous NAA treatment significantly suppresses PR length in osarf7 mutants, OsARF7-OE lines, and its wild-type, does not affect the root number of osarf7 mutants, but suppresses the biomass of osarf7 mutants. At the molecular level, OsARF7 is preferentially expressed in the culm, root, and leaf, especially highly expressed in the tips of the PR, AR, root pericycle, and lateral root (LR) primordia; meanwhile, OsARF7 expression is significantly enhanced by exogenous NAA treatment, suggesting that the positive regulatory role of OsARF7 on root development is based on auxin signaling. A series of biochemical and genetic analyses demonstrate that OsARF7 functions upstream of OsCRL1 and acts downstream of OsMADS25 to regulate root development via auxin signaling. To conclude, OsARF7 is a key positive regulatory factor that regulates root development by activating the expression of OsCRL1 via auxin signaling, by which, OsMADS25 positively mediates OsARF7 expression in rice. This work provides valuable insight into the regulatory mechanism controlling root development and a genetic resource for the molecular improvement of root architecture.
Keyword ：
Auxin signaling Auxin signaling OsARF7 OsARF7 OsCRL1 OsCRL1 Rice Rice Root development Root development
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Sun, Congying , Fan, Kai , Wang, Xin et al. The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.) [J]. | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
| MLA | Sun, Congying et al. "The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.)" . | PLANT MOLECULAR BIOLOGY 115 . 2 (2025) . |
| APA | Sun, Congying , Fan, Kai , Wang, Xin , Liu, Honghai , Guo, Nuoping , Liu, Wanyu et al. The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.) . | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Introduction Lime and biochar are widely utilized to enhance nitrogen utilization in crops grown on acidic soils, though each has its own set of limitations. Understanding their combined effects is crucial for optimizing soil remediation strategies.Methods This study investigates the impact of lime and biochar on nitrogen utilization efficiency (NUE) in a tobacco monoculture system, which has been practiced for 20 years on acidified soils in Fuzhou, southeastern China, over the period from 2021 to 2022. Four treatments were applied: control (CK), lime alone (L), biochar alone (B), and a lime-biochar combination (L+B).Results The results indicated that all treatments significantly improved NUE, with increases ranging from 20.07% to 27.17% compared to CK. Biochar (B) was more effective than lime (L), and the combined treatment (L+B) showed comparable effects to biochar alone. Correlation analysis revealed that increases in soil pH and exchangeable base cations facilitated nitrogen transformation, thereby enhancing NUE. Lime treatments (L, L+B) promoted nitrification potential in rhizosphere soil, whereas biochar application (B, L+B) resulted in elevated nitrate nitrogen content. Microbial functional analysis indicated that lime (L, L+B) enhanced nitrification, while biochar (B, L+B) fostered dissimilatory nitrate reduction, thereby improving nitrogen retention. Pearson correlation analysis demonstrated a strong positive relationship between dissimilatory nitrate reduction and both soil alkali-hydrolyzable nitrogen and nitrate nitrogen contents.Conclusion These findings suggest that lime enhances nitrification, while biochar promotes nitrate retention, together increasing soil nitrogen availability. The combined application of lime and biochar integrates these benefits, yielding results comparable to biochar alone. This study offers valuable insights into the synergistic use of lime and biochar for mitigating soil acidification and optimizing nitrogen management in agricultural systems.
Keyword ：
acidic soil acidic soil biochar biochar lime lime nitrogen utilization efficiency nitrogen utilization efficiency tobacco tobacco
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Bianhong , Tang, Lina , Chen, Zhicheng et al. Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping [J]. | FRONTIERS IN PLANT SCIENCE , 2025 , 16 . |
| MLA | Zhang, Bianhong et al. "Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping" . | FRONTIERS IN PLANT SCIENCE 16 (2025) . |
| APA | Zhang, Bianhong , Tang, Lina , Chen, Zhicheng , Chen, Xiaoyan , You, Lindong , Pan, Ruixin et al. Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping . | FRONTIERS IN PLANT SCIENCE , 2025 , 16 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Exploring and predicting the spatiotemporal evolution characteristics and driving forces of carbon storage in typical mountain forest ecosystems under land-use changes is crucial for curbing the effects of climate change and fostering sustainable, eco-friendly growth. The existing literature provides important references for our related studies but further expansion and improvements are needed in some aspects. This study first proposed an integrated framework comprising gray multi-objective optimization (GMOP), Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST), the Patch-level Land Use Simulation Model (PLUS), and optimal parameter-based geographical detector (OPGD) models to further expand and improve on existing research. Then, the integrated model was used to analyze the spatial-temporal variation in land-use pattern and carbon storage at the county scale in China's Daiyun Mountain's Rim under four scenarios in 2032, and analyze the driving force of spatial differentiation of carbon storage. The results indicated that (1) land-use change primarily involves the mutual transfer among forest, cultivated, and construction land, with approximately 7.2% of the land-use type area undergoing a transition; (2) in 2032, the natural development scenario projects a significant reduction in forest land and an expansion of cultivated, shrub, and construction lands. Conversely, the economic priority, ecological priority, and economic-ecological coordinated scenarios all anticipate a decline in cultivated land area; (3) in 2032, the natural development scenario will see a 2.8 Tg drop in carbon stock compared to 2022. In contrast, the economic priority, ecological priority, and economic-ecological coordinated scenarios are expected to increase carbon storage by 0.29 Tg, 2.62 Tg, and 1.65 Tg, respectively; (4) the spatial differentiation of carbon storage is jointly influenced by various factors, with the annual mean temperature, night light index, elevation, slope, and population density being the key influencing factors. In addition, the influence of natural factors on carbon storage is diminishing, whereas the impact of socioeconomic factors is on the rise. This study deepened, to a certain extent, the research on spatiotemporal dynamics simulation of carbon storage and its driving mechanisms under land-use changes in mountainous forest ecosystems. The results can serve to provide scientific support for carbon balance management and climate adaptation strategies at the county scale while also offering case studies that can inform similar regions around the world. However, several limitations remain, as follows: the singularity of carbon density data, and the research scope being confined to small-scale mountainous forest ecosystems. Future studies could consider collecting continuous annual soil carbon density data and employing land-use simulation models (such as PLUS or CLUMondo) appropriate to the study area's dimensions.
Keyword ：
carbon storage carbon storage GeoDetector GeoDetector GMOP-PLUS-OPGD GMOP-PLUS-OPGD land-use change land-use change multi-scenario simulation multi-scenario simulation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Gui , Peng, Qingxia , Fan, Qiaohong et al. Spatial-Temporal Variation and Driving Forces of Carbon Storage at the County Scale in China Based on a Gray Multi-Objective Optimization-Patch-Level Land Use Simulation-Integrated Valuation of Ecosystem Services and Tradeoffs-Optimal Parameter-Based Geographical Detector Model: Taking the Daiyun Mountain's Rim as an Example [J]. | LAND , 2025 , 14 (1) . |
| MLA | Chen, Gui et al. "Spatial-Temporal Variation and Driving Forces of Carbon Storage at the County Scale in China Based on a Gray Multi-Objective Optimization-Patch-Level Land Use Simulation-Integrated Valuation of Ecosystem Services and Tradeoffs-Optimal Parameter-Based Geographical Detector Model: Taking the Daiyun Mountain's Rim as an Example" . | LAND 14 . 1 (2025) . |
| APA | Chen, Gui , Peng, Qingxia , Fan, Qiaohong , Lin, Wenxiong , Su, Kai . Spatial-Temporal Variation and Driving Forces of Carbon Storage at the County Scale in China Based on a Gray Multi-Objective Optimization-Patch-Level Land Use Simulation-Integrated Valuation of Ecosystem Services and Tradeoffs-Optimal Parameter-Based Geographical Detector Model: Taking the Daiyun Mountain's Rim as an Example . | LAND , 2025 , 14 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Acyl homoserine lactones (AHL), which are extensively studied quorum sensing (QS) signaling molecules in Gram-negative bacteria, play a significant role in regulating plant growth and shaping the rhizosphere ecosystem. In this study, we investigated the effects of continuous planting across multiple generations of the Australian pine Casuarina equisetifolia (Casuarinaceae) on AHL accumulation in its rhizosphere soil. The study aims to identify key AHL and further employs an exogenous supplementation approach to assess their influence on the growth of C. equisetifolia and the associated rhizosphere soil ecosystem. Our findings reveal a progressive increase in total AHL content, rising from 1.76 to 3.65 ng/g with continuous planting generations. The key AHL that significantly alter rhizosphere soil properties under continuous planting conditions are identified as C4-HSL, 3-oxo-C10-HSL, 3-oxo-C12-HSL, and 3-oxo-C14-HSL. Following exogenous treatment with C4-HSL, an increase in its concentration was correlated with a significant enhancement in both the root length and plant height of C. equisetifolia. Conversely, treatments with 3-oxo-C10-HSL, 3-oxo-C12-HSL, and 3-oxo-C14-HSL resulted in a significant reduction in these growth parameters. While all four key AHL contributed positively to the proliferation of soil fungi and actinobacteria, their effects on bacterial populations exhibited variability. Following the exogenous application of the four key AHL, a significant reduction in the activities of urease and protease in the soil was observed. In contrast, the activities of acid phosphatase and cellulase were enhanced, leading to a decrease in the soil's available nitrogen and potassium content, while the available phosphorus content increased. Interaction effect analysis reveals that these key AHL collectively exert a strong positive regulatory effect on soil microbial abundance (0.979**). Furthermore, soil microorganisms show a significant positive correlation with soil enzyme activity (0.997**), whereas soil enzyme activity exhibits a strong negative correlation with the soil's available nutrient content (-0.995**). Additionally, the soil's available nutrient content positively regulates the growth of C. equisetifolia (0.970**). The inhibitory effect of continuous planting on C. equisetifolia growth primarily stems from reduced 3-oxo-C10-HSL levels coupled with elevated 3-oxo-C12-HSL and 3-oxo-C14-HSL concentrations in the rhizosphere. This alteration leads to a decrease in the bacterial population within the soil, which significantly reduces the activities of soil urease and protease, as well as the availability of nitrogen and potassium in the rhizosphere of C. equisetifolia. Consequently, these changes result in markedly diminished root length and dry weight of C. equisetifolia. This study provides a critical theoretical framework for the exogenous application of AHL to modulate C. equisetifolia growth in forest ecological system.
Keyword ：
Acyl homoserine lactones (AHL) Acyl homoserine lactones (AHL) Availability nutrient Availability nutrient Casuarina equisetifolia Casuarina equisetifolia Microbes Microbes Soil enzyme Soil enzyme
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Qingxu , Lin, Yi , Hong, Lei et al. The AHL-driven cascade of rhizosphere microbes, enzymes, and nutrients contributes to the growth decline of Casuarina equisetifolia in continuous planting systems [J]. | RHIZOSPHERE , 2025 , 34 . |
| MLA | Zhang, Qingxu et al. "The AHL-driven cascade of rhizosphere microbes, enzymes, and nutrients contributes to the growth decline of Casuarina equisetifolia in continuous planting systems" . | RHIZOSPHERE 34 (2025) . |
| APA | Zhang, Qingxu , Lin, Yi , Hong, Lei , Wang, Yuhua , Qiu, Miaoen , Li, Jianjuan et al. The AHL-driven cascade of rhizosphere microbes, enzymes, and nutrients contributes to the growth decline of Casuarina equisetifolia in continuous planting systems . | RHIZOSPHERE , 2025 , 34 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The ratoon rice cultivation system exhibits high-yield and low-emission characteristics, with carbon (C) sequestration and emissions in paddy fields being influenced by rhizosphere microorganisms. However, the relationship between these effects and microbial nutritional strategies remains unclear. This study utilized four rice varieties with distinct growth durations as materials to analyze the effects and mechanisms of yield formation, rhizosphere microbial dynamics, and net ecosystem carbon budget (NECB) in the main crop (MC), ratoon season rice (RSR), and single mid-late rice (LR) during 2023-2024. Results showed that the daily average yield of ratoon rice (MC+RSR) increased by 77.65 %-86.82 % compared to LR. Analysis of photosynthate allocation revealed that RSR exhibited 72.09 % and 77.35 % reductions in rhizodeposition compared to MC and LR, respectively. Analysis of microbial trophic strategies revealed a 55.54 % and 32.36 % increase in autotroph abundance in RSR relative to MC and LR. Investigation of greenhouse gas intensity (GHGI) and NECB confirmed that the ratoon rice system (MC+RSR) reduced its daily carbon emission index by 52.74 %-56.08 % compared to LR. The MC+RSR system acted as a carbon sink, sequestering 3.12-10.47 t CO2-eq ha-1 , whereas LR functioned as a carbon source, emitting 3.08-4.48 t CO2-eq ha-1 . Therefore, ratoon rice system extends the utilization period of light and thermal resources, enhances yield, and achieves carbon budget surplus. The increase in autotrophic bacteria and corresponding decrease in heterotrophic bacteria represent a critical rhizosphere microecological resilience response to soil carbon sequestration and emission reduction.
Keyword ：
Carbon balance surplus Carbon balance surplus Emission reduction Emission reduction Rhizosphere microecology Rhizosphere microecology Rice ratooning Rice ratooning
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Jinying , Xu, Hailong , Qin, Bin et al. Mechanisms of high yield formation and carbon budget surplus in ratoon rice and its rhizosphere microecological responses [J]. | FIELD CROPS RESEARCH , 2025 , 333 . |
| MLA | Li, Jinying et al. "Mechanisms of high yield formation and carbon budget surplus in ratoon rice and its rhizosphere microecological responses" . | FIELD CROPS RESEARCH 333 (2025) . |
| APA | Li, Jinying , Xu, Hailong , Qin, Bin , Zhang, Bianhong , Chen, Mengying , Weng, Peiying et al. Mechanisms of high yield formation and carbon budget surplus in ratoon rice and its rhizosphere microecological responses . | FIELD CROPS RESEARCH , 2025 , 333 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Chemotherapy-induced intestinal mucositis is a severe adverse effect affecting cancer patients, and there are currently no effective strategies for its prevention. Fufang Taizishen granules (FFTZS), a traditional Chinese medicine (TCM) known for its anti-inflammatory, anti-fatigue, and immunostimulatory properties, have shown therapeutic potential. However, the effects of FFTZS on chemotherapy-induced intestinal mucositis and its underlying mechanisms remain unclear. A mouse model of intestinal mucositis was induced using 5-FU and irinotecan to assess the protective effects of FFTZS. The study found that FFTZS enhanced food intake, reduced diarrhea index, and improved histopathological damage in colonic tissues. FFTZS also reduced inflammatory cell infiltration and inhibited apoptosis. Moreover, FFTZS treatment increased the expression of Claudin-1 and ZO-1 proteins. Microbial analysis revealed an enrichment of beneficial bacteria and a reduction of opportunistic pathogens, whereas metabolomic analysis showed that FFTZS corrected amino acid metabolic disturbances induced by chemotherapy. These findings suggest that FFTZS mitigates chemotherapy-induced intestinal mucositis by preserving mucosal integrity, modulating gut microbiota, and restoring metabolic homeostasis.
Keyword ：
amino acid metabolism amino acid metabolism Fufang Taizishen granules Fufang Taizishen granules gut microbiota gut microbiota intestinal mucositis intestinal mucositis
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Kan, Yongjun , Liu, Yingying , Zhao, Li et al. Fufang Taizishen Granules Attenuate Chemotherapy-Induced Intestinal Mucositis by Modulating Gut Microbiota and Amino Acid Metabolism [J]. | FOOD SCIENCE & NUTRITION , 2025 , 13 (8) . |
| MLA | Kan, Yongjun et al. "Fufang Taizishen Granules Attenuate Chemotherapy-Induced Intestinal Mucositis by Modulating Gut Microbiota and Amino Acid Metabolism" . | FOOD SCIENCE & NUTRITION 13 . 8 (2025) . |
| APA | Kan, Yongjun , Liu, Yingying , Zhao, Li , Jiang, Chang , Pang, Wensheng , Zhang, Bianhong et al. Fufang Taizishen Granules Attenuate Chemotherapy-Induced Intestinal Mucositis by Modulating Gut Microbiota and Amino Acid Metabolism . | FOOD SCIENCE & NUTRITION , 2025 , 13 (8) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Polyphenol oxidase (PPO) oxidizes polyphenols, causing browning in tobacco (Nicotiana tabacum L.) leaves during roasting, which reduces their quality and commercial value. In this study, 13 NtPPO genes were identified through genome mining, and their bioinformatic analyses were performed, including gene structure, protein structure, and physicochemical properties. Tissue-specific expression analysis found that the NtPPO genes were substantially expressed in leaves, whereas NtPPO9 and NtPPO10 were also highly expressed in flowers. During leaf growth, NtPPO1, 3, 5, 6, and 7 expression levels changed dramatically, whereas NtPPO2, 9, 10, and 13 expression patterns remained steady. Notably, NtPPO1 displayed the highest expression levels during the maturity stage of tobacco leaves. Subcellular localization revealed that NtPPO1, 6, 9, and 12 were primarily distributed in chloroplasts. KO-87 was generated through CRISPR/Cas9 technology, including mutations in the NtPPO1, 2, 4, 6, 8, 9, 11, 12, and 13 genes. KO-87 exhibits similar growth to the wild-type, but with decreased PPO activity in leaves and browning levels after roasting. Metabolomics analysis indicated that polyphenol metabolites were significantly upregulated, while quinone metabolites were significantly downregulated in the leaves of mutant plants. This study systematically explored the functions of NtPPO genes, providing preliminary insights into their key roles in the browning process of tobacco leaves. These findings offer new research directions and potential solutions for addressing the browning problem during tobacco leaf roasting.
Keyword ：
Browning of tobacco leaves Browning of tobacco leaves CRISPR/Cas9 CRISPR/Cas9 NtPPOs NtPPOs Tobacco Tobacco
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Shixu , Lin, Yingchao , Zeng, Xinhai et al. Genome-wide characterization of polyphenol oxidase (PPO) gene family in tobacco and the molecular improvement of browning reaction for K326 tobacco leaves [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 235 . |
| MLA | Zhang, Shixu et al. "Genome-wide characterization of polyphenol oxidase (PPO) gene family in tobacco and the molecular improvement of browning reaction for K326 tobacco leaves" . | INDUSTRIAL CROPS AND PRODUCTS 235 (2025) . |
| APA | Zhang, Shixu , Lin, Yingchao , Zeng, Xinhai , Liu, Guangju , Ye, Guixiang , Du, Changyang et al. Genome-wide characterization of polyphenol oxidase (PPO) gene family in tobacco and the molecular improvement of browning reaction for K326 tobacco leaves . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 235 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Studying the physioecological mechanisms behind the high daily productivity of ratoon rice is essential for improving farming strategies and maintaining stable yields in intensive cropping systems. This study compared the physiological traits of main crop rice (MR), ratooning season rice (RSR), and single-cropping mid-late rice (LR) with the same genotype and flowering time as RSR, from 2021 to 2022. The results demonstrated that RSR maintained higher leaf SPAD values than both MR and LR at the heading stage. Moreover, the translocation efficiency of stem-stored non-structural carbohydrates (NSC) to grains was significantly greater in RSR compared to MR (24.18-58.1%) and LR (24.59-63.5%). C-13 isotope labeling confirmed that RSR exhibited superior assimilate allocation to grain pools, with values 13.7% and 14.74% higher than those of MR and LR, respectively. Anatomical analysis of vascular bundles at panicle-stem nodes showed an increased phloem area proportion in RSR. Furthermore, RSR exhibited elevated expression levels of genes associated with nitrogen uptake and metabolism regulation (AMTs, NRTs, and NRFs), sucrose transport (SUTs, SWEET11, and INVs), and stress tolerance (TPSs and TPPs), particularly within its grain parts, relative to MR and LR. Enzymatic activities of sucrose synthase (SS), sucrose phosphate synthase (SPS), and invertase in RSR grains were also significantly higher than in MR and LR. Further analysis indicated that these advantageous physiological traits in RSR arise from the elevated gene expression and enzyme activities, which are positively modulated by endogenous hormones including zeatin riboside (ZR), abscisic acid (ABA), and indole-3-acetic acid (IAA). These hormones serve as key factors for the efficient coordination of matter production and translocation in rice ratooning. This study provides important theoretical and practical insights into the formation and regulation mechanisms of high and stable yield in ratoon rice.
Keyword ：
Dry matter accumulation Dry matter accumulation Enzymatic activity Enzymatic activity Material transport Material transport Phytohormone Phytohormone Ratoon rice Ratoon rice Translocation Translocation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xie, Yuebin , Zou, Jingnan , Cao, Shuying et al. The Higher Average Daily Grain Yield and Its Formation Mechanisms Underlying the Efficient Photo-Assimilate Transport in Rice Ratooning [J]. | RICE , 2025 , 18 (1) . |
| MLA | Xie, Yuebin et al. "The Higher Average Daily Grain Yield and Its Formation Mechanisms Underlying the Efficient Photo-Assimilate Transport in Rice Ratooning" . | RICE 18 . 1 (2025) . |
| APA | Xie, Yuebin , Zou, Jingnan , Cao, Shuying , Lan, Chaojie , Qin, Bin , Xu, Hailong et al. The Higher Average Daily Grain Yield and Its Formation Mechanisms Underlying the Efficient Photo-Assimilate Transport in Rice Ratooning . | RICE , 2025 , 18 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
