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Role of KOH-activated biochar on promoting anaerobic digestion of biomass from Pennisetum gianteum SCIE
期刊论文 | 2024 , 353 | JOURNAL OF ENVIRONMENTAL MANAGEMENT
WoS CC Cited Count: 10
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Abstract :

Pennisetum giganteum is a promising non-food crop feedstock for biogas production due to its high productivity and bio-methane potential. However, the accumulation of volatile fatty acids (VFA) usually restricts the conversion efficiency of P. giganteum biomass (PGB) during anaerobic digestion (AD). Here, the role of KOHactivated biochar (KB) in improving the AD efficiency of PGB and the related mechanisms were investigated in detail. The results revealed that KB exhibited excellent electrical conductivity, electron transfer capacity and specific capacitance, which might be related to the decrease in the electron transfer resistance after adding KB to the AD process. In addition, the KB addition not only reinforced metabolisms of energy and VFAs but also promoted the conversion of VFAs to methane, leading to a 52% increase in the methane production rate. Bioinformatics analysis showed that Smithella and Methanosaeta were key players in the KB-mediated AD process of PGB. The stimulatory effect of methanogenesis probably resulted from the establishment of direct interspecies electron transfer (DIET) between VFA-oxidizing acetogens (e.g., Smithella) and Methanosaeta. These findings provided a key step to improve the PGB-based AD process.

Keyword :

Anaerobic digestion Anaerobic digestion Biochar Biochar Methane Methane Pennisetum gianteum Pennisetum gianteum

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GB/T 7714 Liu, Qing-Hua , Sun, Hong-Ying , Yang, Zhi-Man . Role of KOH-activated biochar on promoting anaerobic digestion of biomass from Pennisetum gianteum [J]. | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2024 , 353 .
MLA Liu, Qing-Hua 等. "Role of KOH-activated biochar on promoting anaerobic digestion of biomass from Pennisetum gianteum" . | JOURNAL OF ENVIRONMENTAL MANAGEMENT 353 (2024) .
APA Liu, Qing-Hua , Sun, Hong-Ying , Yang, Zhi-Man . Role of KOH-activated biochar on promoting anaerobic digestion of biomass from Pennisetum gianteum . | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2024 , 353 .
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盐碱胁迫对巨菌草根际土壤微生物多样性及酶活性的影响
期刊论文 | 2022 , 42 (03) , 491-500 | 广西植物
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Abstract :

为探明盐碱胁迫对巨菌草根际土壤微生物多样性及酶活性的影响,该文设置了7个盐碱梯度,利用SPSS和Illumina高通量测序分析巨菌草根际土壤生理指标和微生物多样性。结果表明:(1)各处理间,巨菌草根际土壤真菌和细菌结构均存在差异;12‰盐碱浓度下,真菌优势菌纲为粪壳菌纲(68.5%)、散囊菌纲(16.3%),细菌优势菌纲为绿弯菌纲(8.5%)、拟杆菌纲(13.5%)、芽单胞菌纲(5.6%)和δ-变形菌纲(3.7%);6‰盐碱浓度时,真菌优势菌纲为座囊菌纲(27.7%),细菌优势菌纲为厌氧绳菌纲(2.4%),当盐碱浓度提高至12‰时,该优势菌群的相对丰度分别降至2.5%和0.007%;真菌和细菌群落Chao指数和Shannon指数随着盐碱浓度的升高而降低,且与土壤盐碱浓度显著相关(P<0.01)。(2)低浓度盐碱胁迫对巨菌草根际土壤脲酶、多酚氧化酶、碱性磷酸酶活性均有促进作用,当盐碱浓度超过8‰时,土壤多酚氧化酶、碱性磷酸酶、蔗糖酶活性受到抑制;种植巨菌草后,土壤有机质含量显著提高(P<0.05)且盐碱浓度显著降低(P<0.05);种植巨菌草对土壤盐碱度的中和能力在中高浓度下较弱;盐碱浓度为6‰时,巨菌草产量最高,且土壤中的速效养分和有机质含量均高于其他盐碱浓度。该研究表明,巨菌草能通过调节酶的浓度来适应中低浓度盐碱环境,且在不同浓度的盐碱土壤中,产生相应的优势菌群来适应该环境,种植巨菌草对中低浓度盐碱土壤有一定改良作用。

Keyword :

土壤微生物 土壤微生物 土壤肥力 土壤肥力 理化性质 理化性质 盐碱胁迫 盐碱胁迫 耐盐植物 耐盐植物

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GB/T 7714 严少娟 , 刘怡萌 , 孙艳丽 et al. 盐碱胁迫对巨菌草根际土壤微生物多样性及酶活性的影响 [J]. | 广西植物 , 2022 , 42 (03) : 491-500 .
MLA 严少娟 et al. "盐碱胁迫对巨菌草根际土壤微生物多样性及酶活性的影响" . | 广西植物 42 . 03 (2022) : 491-500 .
APA 严少娟 , 刘怡萌 , 孙艳丽 , 林兴生 , 罗海凌 , 林辉 et al. 盐碱胁迫对巨菌草根际土壤微生物多样性及酶活性的影响 . | 广西植物 , 2022 , 42 (03) , 491-500 .
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Effects of emerged plant on soil methane emission and nitrogen content in constructed wetland CPCI-S
期刊论文 | 2019 , 218 | 2018 INTERNATIONAL CONFERENCE ON CIVIL, ARCHITECTURE AND DISASTER PREVENTION
WoS CC Cited Count: 2
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Abstract :

To find the effects of emerged plant on soil methane (CH4) emission and nitrogen content in constructed wetland, we constructed the constructed wetland microcosms with 3 treatments through 2 emerged plants (Typha orientalis and Phragmites ausfralis). Results showed that soil CH4 flux have no different (2.0, 2.4, 2.5 mg m(2) d(-1), P > 0.05), but soil nitrogen contents and microbial biomass nitrogen were significantly different (P < 0. 01) among the 3 treatments. The soil ammonium nitrogen (P < 0.001) and nitrate nitrogen (P < 0.01) contents in the planted constructed wetland were lower than in those unplanted constructed wetland, but microbial biomass nitrogen (MBN) were higher (P < 0.01). The soil CH4 flux, microbial biomass, organic matter and nitrogen content had no different between those constructed wetlands planted with Typha orientalis and Phragmites australis (P > 0.05). As total plant biomass in the constructed wetlands planted with Typha orientalis (P < 0.001) were higher than that in the constructed wetlands planted with Phragmites ausfralis (P < 0.05). Hence, emerged plant regulate soil nitrogen removal but not affect soil CH4 emission in these systems.

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GB/T 7714 Sun, Hongying , Xin, Quanwei , Luo, Hailing et al. Effects of emerged plant on soil methane emission and nitrogen content in constructed wetland [J]. | 2018 INTERNATIONAL CONFERENCE ON CIVIL, ARCHITECTURE AND DISASTER PREVENTION , 2019 , 218 .
MLA Sun, Hongying et al. "Effects of emerged plant on soil methane emission and nitrogen content in constructed wetland" . | 2018 INTERNATIONAL CONFERENCE ON CIVIL, ARCHITECTURE AND DISASTER PREVENTION 218 (2019) .
APA Sun, Hongying , Xin, Quanwei , Luo, Hailing , Yan, Shaojuan , Lan, Siren , Wang, Yan . Effects of emerged plant on soil methane emission and nitrogen content in constructed wetland . | 2018 INTERNATIONAL CONFERENCE ON CIVIL, ARCHITECTURE AND DISASTER PREVENTION , 2019 , 218 .
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Effects of plant diversity on carbon dioxide emissions and carbon removal in laboratory-scale constructed wetland SCIE
期刊论文 | 2019 , 26 (5) , 5076-5082 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
WoS CC Cited Count: 27
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Abstract :

Previous studies have shown that plant diversity can enhance methane (CH4) emission and nitrogen purification efficiency in constructed wetlands (CWs), but effect of plant diversity on carbon dioxide (CO2) flux and carbon removal efficiency in CWs is unknown. Therefore, we established four plant diversity levels (each level containing 4, 3, 2, and 1 species, respectively) in laboratory-scale wetland microcosms fed with simulated wastewater. Results showed that plant species richness enhanced CO2 emissions (84.7-124.7 mg CO2 m(-2) h(-1), P < 0.01), carbon fixation rate (P < 0.05), and microbial biomass carbon (P < 0.001), but did not improve carbon removal (P > 0.05). The presence of Pontederia cordata increased CO2 emissions, carbon fixation rate of belowground, and microbial biomass carbon (P < 0.05), whereas the presence of Phragmites australis only enhanced CO2 emission (P < 0.05). However, the presence of Typha orientalis or Lythrum salicaria did not show an influence on CO2 emissions and carbon removal (P > 0.05). Hence, our study highlights the importance of plant diversity in mediating CO2 emission intensity and carbon processes but not carbon removal in CWs.

Keyword :

Carbon fixation rate Carbon fixation rate Microbial biomass Microbial biomass Microcosms Microcosms Plant diversity Plant diversity Plant species Plant species Species richness Species richness

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GB/T 7714 Sun, Hongying , Xin, Quanwei , Ma, Zhihui et al. Effects of plant diversity on carbon dioxide emissions and carbon removal in laboratory-scale constructed wetland [J]. | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 2019 , 26 (5) : 5076-5082 .
MLA Sun, Hongying et al. "Effects of plant diversity on carbon dioxide emissions and carbon removal in laboratory-scale constructed wetland" . | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 26 . 5 (2019) : 5076-5082 .
APA Sun, Hongying , Xin, Quanwei , Ma, Zhihui , Lan, Siren . Effects of plant diversity on carbon dioxide emissions and carbon removal in laboratory-scale constructed wetland . | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 2019 , 26 (5) , 5076-5082 .
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人工湿地植物种类及多样性对甲烷释放及功能基因丰度的影响 CSCD PKU
期刊论文 | 2019 , 39 (22) , 8565-8574 | 生态学报
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为了研究人工湿地处理中碳/氮水平的废水时植物种类及多样性对系统甲烷释放及功能基因丰度的影响,我们构建了实验尺度的人工湿地微宇宙实验系统。选取千屈菜(Lythrum salicaria L.)和海寿花(Pontederia cordata L.)2种人工湿地常用、景观效果好的植物,在系统中配置了单种处理和两物种混种处理。结果表明:千屈菜与海寿花混种系统的甲烷释放强度(8.78 mg CH_4 m~(-2) d~(-1))高于两物种单种系统的平均值(6.97 mg CH_4 m~(-2) d~(-1))(P<0.001),同甲烷释放一样,混种系统的mcrA基因绝对丰度(977541.6 copies/g dw soil)也高于两物种单种系统的平均值(585146.8 copies/g dw soil),但混种系统的pmoA基因绝对丰度(326956.6 copies/g dw soil)低于两物种单种系统的平均值(1043616.0 copies/g dw soil)(P<0.001)。此外,混种系统的微生物量、植物生物量高于两物种单种系统的平均值(P<0.01),但出水铵态氮浓度低于两物种单种系统的平均值(P<0.05),出水总有机碳浓度和硝态氮浓度在单混种系统间无显著差异(P>0.05)。千屈菜单种系统和海寿花单种系统间的甲烷释放强度、pmoA基因绝对丰度、微生物量、植物生物量和出水铵态氮浓度存在显著差异(P<0.05),但mcrA基因绝对丰度、出水总有机碳和硝态氮浓度无显著差异(P>0.05)。为了达到人工湿地的高净化效率,需要将千屈菜与海寿花混合种植,但混合种植强化甲烷释放。通过植物种类和丰富度对各指标变异的解释度(ω~2)分析发现,植物种类对甲烷释放、pmoA基因绝对丰度、出水铵态氮的影响大于植物丰富度,但对mcrA基因绝对丰度的影响小于植物丰富度。

Keyword :

mcrA基因 mcrA基因 pmoA基因 pmoA基因 温室气体 温室气体 生态系统功能 生态系统功能 生物多样性 生物多样性

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GB/T 7714 孙红英 , 辛全伟 , 林兴生 et al. 人工湿地植物种类及多样性对甲烷释放及功能基因丰度的影响 [J]. | 生态学报 , 2019 , 39 (22) : 8565-8574 .
MLA 孙红英 et al. "人工湿地植物种类及多样性对甲烷释放及功能基因丰度的影响" . | 生态学报 39 . 22 (2019) : 8565-8574 .
APA 孙红英 , 辛全伟 , 林兴生 , 罗海凌 , 林辉 , 严少娟 et al. 人工湿地植物种类及多样性对甲烷释放及功能基因丰度的影响 . | 生态学报 , 2019 , 39 (22) , 8565-8574 .
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珍珠彩桂的快速繁殖技术 CSCD PKU
期刊论文 | 2019 , 39 (03) , 287-291 | 森林与环境学报
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以珍珠彩桂半木质化枝条为外植体,采用丛生芽诱导途径,建立组培快繁体系。结果表明:最佳启动培养基为木本植物培养基(WPM)+2.0 mg·L~(-1)6-苄氨基腺嘌呤(6-BA)+0.5 mg·L~(-1)赤霉素(GA3)+6.5 g·L~(-1)琼脂+30 g·L~(-1)蔗糖,启动率高达86.4%;最佳增殖培养基为WPM+4.0 mg·L~(-1)6-BA+2.0 mg·L~(-1)6-糖基氨基嘌呤(KT)+0.5 mg·L~(-1)GA3+6.5 g·L~(-1)琼脂+30 g·L~(-1)蔗糖,继代周期25 d,增殖系数高达5.8;最佳生根培养基为1/2WPM+3.0 mg·L~(-1)3-吲哚丁酸(IBA)+6.5 g·L~(-1)琼脂+30 g·L~(-1)蔗糖,培养30 d后生根,生根率达89.5%。炼苗后,移栽到珍珠岩∶蛭石∶泥炭土体积比为1∶1∶1的混合基质中,成活率达到90%以上。

Keyword :

半木质化茎 半木质化茎 珍珠彩桂 珍珠彩桂 组织培养 组织培养

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GB/T 7714 孙红英 , 辛全伟 , 兰思仁 . 珍珠彩桂的快速繁殖技术 [J]. | 森林与环境学报 , 2019 , 39 (03) : 287-291 .
MLA 孙红英 et al. "珍珠彩桂的快速繁殖技术" . | 森林与环境学报 39 . 03 (2019) : 287-291 .
APA 孙红英 , 辛全伟 , 兰思仁 . 珍珠彩桂的快速繁殖技术 . | 森林与环境学报 , 2019 , 39 (03) , 287-291 .
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速生白榆的组织培养与快速繁殖 CSCD PKU
期刊论文 | 2019 , 39 (12) , 1593-1598 | 广西植物
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该文以速生白榆半木质化枝条为外植体,使用75%的酒精和0.1%HgCl_2消毒处理,外植体经过启动培养后,在增殖培养基中进行丛生芽诱导,将丛生芽切成单株进行生根诱导,最终建立起成熟的速生白榆组培快繁体系。结果表明:外植体最佳消毒处理组合为75%的酒精处理50 s+0.1%HgCl_2处理8 min,外植体污染率为17.3%,成活率为78%;将消毒处理过的外植体接种到启动培养基中,培养25 d,最终筛选出最适白榆外植体启动的培养基为MS+1.0 mg·L~(-1)6-BA+0.1 mg·L~(-1)IBA+30 g·L~(-1)蔗糖+6.5 g·L~(-1)琼脂,启动率高达87.5%;将经过启动培养后的外植体腋芽切下,接种到增殖培养基中进行丛生芽诱导,最终筛选出最佳增殖培养基为MS+0.5 mg·L~(-1)6-BA+0.1 mg·L~(-1)KT+0.1 mg·L~(-1)IBA+30 g·L~(-1)蔗糖+6.5 g·L~(-1)琼脂,继代周期25 d,增殖系数达6.2;将丛生芽切成单株,接种到生根诱导培养基中,筛选出最佳生根培养基为1/2 MS+0.1 mg·L~(-1)IBA+0.1 mg·L~(-1)IAA+30 g·L~(-1)蔗糖+6.5 g·L~(-1)琼脂,生根诱导30 d,生根率达97%。将生根苗在室外炼苗后,移栽到珍珠岩∶蛭石∶泥炭土体积比为1∶1∶1的混合基质中,成活率在90%以上。较高的增殖系数、生根率和移栽成活率可以降低生产成本,进而实现工厂化育苗。

Keyword :

快速繁殖 快速繁殖 最佳培养基 最佳培养基 组织培养 组织培养 速生白榆 速生白榆

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GB/T 7714 孙红英 , 辛全伟 , 罗海凌 et al. 速生白榆的组织培养与快速繁殖 [J]. | 广西植物 , 2019 , 39 (12) : 1593-1598 .
MLA 孙红英 et al. "速生白榆的组织培养与快速繁殖" . | 广西植物 39 . 12 (2019) : 1593-1598 .
APA 孙红英 , 辛全伟 , 罗海凌 , 马志慧 , 严少娟 . 速生白榆的组织培养与快速繁殖 . | 广西植物 , 2019 , 39 (12) , 1593-1598 .
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中红杨离体叶片植株再生体系建立 CSCD PKU
期刊论文 | 2019 , 39 (04) , 28-31 | 中南林业科技大学学报
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Abstract :

以中红杨组培苗的叶片为试验材料,研究了基本培养基、植物生长调节物质浓度、外源添加物和光照条件对离体叶片不定芽再生的影响,并获得了完整的再生植株。结果表明:1)最适基本培养基为MS,最佳植物生长调节剂和外源添加物的组合为0.5 mg·L~(-1) 6-BA+0.2 mg·L~(-1) NAA+30 g·L~(-1)蔗糖;2)暗培养有利于愈伤组织诱导,光照16 h·d~(-1)有利于不定芽的诱导,不定芽形成率可达83.3%;3)将叶片再生植株转接到MS+0.2 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA培养基中壮苗培养,在1/2 MS+0.2 mg·L~(-1) IBA+20 g·L~(-1)蔗糖+6.5 g·L~(-1)琼脂培养基诱导生根,生根率96.4%,生根苗大田移栽成活率86.8%。

Keyword :

中红杨 中红杨 植株再生 植株再生 生根成活 生根成活 离体叶片培养 离体叶片培养

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GB/T 7714 孙红英 , 辛全伟 , 马志慧 et al. 中红杨离体叶片植株再生体系建立 [J]. | 中南林业科技大学学报 , 2019 , 39 (04) : 28-31 .
MLA 孙红英 et al. "中红杨离体叶片植株再生体系建立" . | 中南林业科技大学学报 39 . 04 (2019) : 28-31 .
APA 孙红英 , 辛全伟 , 马志慧 , 兰思仁 . 中红杨离体叶片植株再生体系建立 . | 中南林业科技大学学报 , 2019 , 39 (04) , 28-31 .
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日本红枫‘青龙’组织培养与快速繁殖 CSCD PKU
期刊论文 | 2019 , 39 (07) , 44-47 | 中南林业科技大学学报
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以青龙半木质化枝条为外植体,采用丛生芽诱导途径,建立起组培快繁体系。结果表明:最佳启动培养基为WPM+0.05 mg/LTDZ+30 g/L蔗糖+6.5 g/L琼脂,启动率为96.1%;最佳增殖培养基为WPM+0.06 mg/L TDZ+0.3 mg/L IBA+6.5 g/L琼脂+30 g/L蔗糖,继代周期30 d,增殖系数高达4.8;最佳生根培养基为1/2WPM+0.3 mg/L NAA+6.5 g/L琼脂+30 g/L蔗糖,培养30 d,其生根率达96.7%。炼苗后,移栽到珍珠岩∶蛭石∶泥炭土体积比为1∶1∶1的混合基质中,成活率达到90%以上。

Keyword :

快速繁殖 快速繁殖 日本红枫‘青龙’ 日本红枫‘青龙’ 最佳培养基 最佳培养基 组织培养 组织培养

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GB/T 7714 孙红英 , 辛全伟 , 林兴生 et al. 日本红枫‘青龙’组织培养与快速繁殖 [J]. | 中南林业科技大学学报 , 2019 , 39 (07) : 44-47 .
MLA 孙红英 et al. "日本红枫‘青龙’组织培养与快速繁殖" . | 中南林业科技大学学报 39 . 07 (2019) : 44-47 .
APA 孙红英 , 辛全伟 , 林兴生 , 罗海凌 , 林辉 , 马志慧 et al. 日本红枫‘青龙’组织培养与快速繁殖 . | 中南林业科技大学学报 , 2019 , 39 (07) , 44-47 .
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人工湿地植物种类和多样性对氧化亚氮释放及功能基因丰度的影响 CSCD PKU
期刊论文 | 2019 , 38 (08) , 2310-2317 | 生态学杂志
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Abstract :

为了解人工湿地处理中碳/氮水平的废水时植物种类及多样性对系统氧化亚氮释放及功能基因丰度的影响,本研究构建了实验尺度的垂直流人工湿地微宇宙实验系统。选取芦苇(Phragmites australis)、千屈菜(Lythrum salicaria)和海寿花(Pontederia cordata) 3种人工湿地常用、景观效果好的植物,在系统中配置了3个单种处理和1个三物种混种处理。结果表明:芦苇、千屈菜与海寿花混种系统的氧化亚氮释放强度(24597.0μg N_2O·m~(-2)·d~(-1))高于三物种单种系统的平均值(11744.8μg N_2O·m~(-2)·d~(-1))(P<0.001),同氧化亚氮释放一样,混种系统的amoA基因绝对丰度(6.33×10~7copies·g~(-1)soil)和nirS基因绝对丰度(1.92×10~6copies·g~(-1)soil)也高于三物种单种系统的平均值(5.70×10~7和1.58×10~6copies·g~(-1)soil)。此外,混种系统的出水硝态氮浓度低于三物种单种系统的平均值(P<0.05),但出水硝态氮浓度、微生物量和植物生物量在单混种系统间无显著差异(P>0.05)。3个单种系统间的氧化亚氮释放强度、amoA基因绝对丰度、nirS基因绝对丰度、出水铵态氮浓度、微生物量和植物生物量存在显著差异(P<0.01),但出水硝态氮无显著差异(P>0.05)。通过植物种类和丰富度对各指标变异的解释度发现,植物种类和丰富度分别解释变异的比率存在一定差异,总体上,植物丰富度对氧化亚氮释放、amoA基因绝对丰度和nirS基因绝对丰度的影响大于植物种类,植物种类对出水硝态氮浓度的影响大于植物丰富度。

Keyword :

amoA基因 amoA基因 nirS基因 nirS基因 温室气体 温室气体 生态系统功能 生态系统功能 生物多样性 生物多样性

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GB/T 7714 孙红英 , 辛全伟 , 林兴生 et al. 人工湿地植物种类和多样性对氧化亚氮释放及功能基因丰度的影响 [J]. | 生态学杂志 , 2019 , 38 (08) : 2310-2317 .
MLA 孙红英 et al. "人工湿地植物种类和多样性对氧化亚氮释放及功能基因丰度的影响" . | 生态学杂志 38 . 08 (2019) : 2310-2317 .
APA 孙红英 , 辛全伟 , 林兴生 , 罗海凌 , 林辉 , 马志慧 et al. 人工湿地植物种类和多样性对氧化亚氮释放及功能基因丰度的影响 . | 生态学杂志 , 2019 , 38 (08) , 2310-2317 .
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