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学者姓名：吴林坤
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Background and aimsThe long-term monoculture of tea plants leads to the accumulation of soil pathogens, disruption of the ecological balance of soil microbial communities. Intercropping systems, have been shown to enhance soil ecological stability. However, there is still a lack of research on the effects of the tea plant/Ophiopogon japonicus intercropping system on soil nutrient cycling and key microbial communities. MethodsA three-year field experiment was conducted to compare two treatments, monoculture and intercropping. Growth parameters such as tea plant height, canopy width, and bud number were investigated. High-throughput sequencing was used to evaluate the impact of the intercropping system on soil microbial community structure and function. ResultsThe intercropping system significantly promotes tea plant growth, with plant height, bud number, and fresh weight increasing by an average of 10.87%, 24.26%, and 23.53%, respectively. Soil organic matter content increased by an average of 23.83%. Simultaneously, the diversity and symbiotic relationships of soil microbial communities continue to strengthen, with significant enrichment observed in Proteobacteria, and Basidiomycota. Random forest modeling further indicated that intercropping enhanced the abundance of microbial taxa involved in organic matter decomposition, such as Pseudomonas, Sphingomonas, and Archaeorhizomyces, consistent with significant increases in cellulase and polyphenol oxidase activities (P < 0.05). Additionally, the intercropping system significantly reduced the relative abundance of pathogenic fungi such as Cladosporium and Curvularia. ConclusionIn tea cultivation practices, intercropping tea plants with O. japonicus can improve the soil micro-ecology of mountain tea plantations, restore soil vitality, and promote tea plant growth.
Keyword ：
High-throughput sequencing High-throughput sequencing Intercropping Intercropping Soil microbiota Soil microbiota Tea plant/Ophiopogon japonicas Tea plant/Ophiopogon japonicas
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| GB/T 7714 | Shao, Shuaibo , Li, Zhongwei , Ma, Xiaoxiao et al. Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus [J]. | PLANT AND SOIL , 2025 , 513 (2) : 2807-2825 . |
| MLA | Shao, Shuaibo et al. "Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus" . | PLANT AND SOIL 513 . 2 (2025) : 2807-2825 . |
| APA | Shao, Shuaibo , Li, Zhongwei , Ma, Xiaoxiao , Cui, Jingru , Zhu, Yanqi , Li, Yuanping et al. Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus . | PLANT AND SOIL , 2025 , 513 (2) , 2807-2825 . |
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Under long-term continuous cropping, Achyranthes bidentata sustained higher yield and quality by maintaining a stable microbial community that fosters positive plant-soil feedback and demonstrates ecological resilience. Our study elucidates how long-term monoculture alters microbial communities across the soil-root continuum. Bacterial diversity increased in both the rhizosphere and endosphere, while fungal diversity decreased in the rhizoplane and endosphere, illustrating a pronounced ecological divergence between bacterial and fungal communities. Bacteria exhibited niche expansion, shifting from K-strategists to r-strategists, adopting more competitive resource-acquisition strategies. In contrast, fungi became increasingly resource-specialized: symbiotic taxa dominated root compartments, while pathogenic taxa accumulated in the rhizoplane and endosphere. Community assembly shifted from stochastic processes to environmental filtering, particularly in the endosphere, reflecting intensified selection pressures over time. Network analysis pinpointed keystone taxa, most notably Pseudomonas spp., that may stabilize microbial networks and sustain ecosystem functions under monoculture stress. Soil sterilization decreased A. bidentata biomass accumulation by 43 % and reduced bioactive compounds by 25.71 % (beta-ecdysterone), 28.57 % (25R-inokosterone), and 25 % (25S-inokosterone) (P < 0.05). Exogenous inoculation with the plant growth-promoting rhizobacteria strain Pseudomonas aeruginosa enhanced root fresh weight by 33.88 % compared to the non-inoculated control without significantly altering ecdysteroid profiles. These findings provide valuable insights into microbial adaptations to monoculture, offering strategies for managing soil microbiomes to improve crop resilience and sustainable agroecosystem management.
Keyword ：
Co-occurrence networks Co-occurrence networks Ecological resilience Ecological resilience Life-history strategies Life-history strategies Microbial communities Microbial communities Root-soil continuum Root-soil continuum
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| GB/T 7714 | Liu, Yazhou , Zeng, Chunli , Zhang, Bianhong et al. Microbially mediated ecological resilience in the root-soil continuum underlies Achyranthes bidentata adaptation to continuous cropping [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 235 . |
| MLA | Liu, Yazhou et al. "Microbially mediated ecological resilience in the root-soil continuum underlies Achyranthes bidentata adaptation to continuous cropping" . | INDUSTRIAL CROPS AND PRODUCTS 235 (2025) . |
| APA | Liu, Yazhou , Zeng, Chunli , Zhang, Bianhong , Zhang, Chenjing , Jiao, Yanyang , Yang, Kaiwen et al. Microbially mediated ecological resilience in the root-soil continuum underlies Achyranthes bidentata adaptation to continuous cropping . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 235 . |
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As sustainable forest management gains increasing attention, comprehending the impact of stand density on soil properties and microbial communities is crucial for optimizing forest ecosystem functions. This study employed high-throughput sequencing in conjunction with soil physicochemical analysis to assess the effects of stand density on soil physicochemical properties and microbial community characteristics in Chinese fir plantations, aiming to elucidate the influence of density regulation on ecosystem services. Our results suggested that changes in soil physicochemical properties and microenvironmental conditions were key drivers of soil microbial diversity. Total carbon (TC), soluble nitrogen (SN), and light fraction organic matter decreased with increasing stand density, while total potassium (TK) and available phosphorus (AP) concentrations increased. The plot with a density of 900 trees ha-1 exhibited the highest bacterial diversity, in contrast to the plot with 1500 trees ha-1, which showed the lowest. The dominant microbial taxa were similar across different stand retention densities, with Acidobacteria, Proteobacteria, and Chloroflexi being the predominant bacterial phyla and Ascomycota and Basidiomycota being the main fungal groups. Significant positive correlations were observed between soil microbial community structures and environmental factors, particularly with respect to soil phosphorus and nitrogen content. The present study demonstrated that reduced stand densities modulated soil nutrient content and enhanced bacterial diversity, thereby contributing to a more complex and stable soil ecosystem structure. These insights provide a scientific foundation for optimizing the management of Chinese fir plantations, thereby supporting the sustainable development of forest ecosystems.
Keyword ：
Chinese fir Chinese fir high-throughput sequencing high-throughput sequencing microbial diversity microbial diversity plantation forest plantation forest soil microorganisms soil microorganisms
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| GB/T 7714 | Xu, Zuyuan , Fan, Fei , Lin, Qinmin et al. Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation [J]. | PLANTS-BASEL , 2025 , 14 (1) . |
| MLA | Xu, Zuyuan et al. "Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation" . | PLANTS-BASEL 14 . 1 (2025) . |
| APA | Xu, Zuyuan , Fan, Fei , Lin, Qinmin , Guo, Shengzhou , Li, Shumao , Zhang, Yunpeng et al. Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation . | PLANTS-BASEL , 2025 , 14 (1) . |
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The monoculture planting in terraced tea plantations has led to severe soil degradation, which poses a significant threat to the growth of tea plants. However, the mechanisms by which intercropping systems improve soil health through the regulation of soil microbial communities at the micro-topographical scale of terraced tea plantations (i.e., terrace surface, inter-row, and terrace wall) remain unclear. This study investigates the effects of intercropping Ophiopogon japonicus in a five-year tea plantation on the soil physicochemical properties, enzyme activities, and microbial community structure and functions across different micro-topographical features of terraced tea plantations in Wuyi Mountain. The results indicate that intercropping significantly improved the soil organic matter, available nutrients, and redox enzyme activities in the inter-row, terrace surface, and terrace wall, with the effects gradually decreasing with increasing distance from the tea plant rhizosphere. In the intercropping group, tea leaf yield increased by 13.17% (fresh weight) and 19.29% (dry weight) compared to monoculture, and the disease indices of new and old leaves decreased by 40.63% and 38.7%, respectively. Intercropping strengthened the modularity of bacterial networks and the role of stochasticity in shaping bacterial communities in different micro-topographic environments, in contrast to the patterns observed in fungal communities. The importance of microbial phyla such as Proteobacteria and Ascomycota in different micro-topographical features was significantly regulated by intercropping. In different micro-topographical zones of the terraced tea plantation, beneficial bacterial genera such as Sinomonas, Arthrobacter, and Ferruginibacter were significantly enriched, whereas potential fungal pathogens like Nigrospora, Microdochium, and Periconia were markedly suppressed. Functional annotations revealed that nitrogen cycling functions were particularly enhanced in inter-row soils, while carbon cycling functions were more prominent on the terrace surface and wall. This study sheds light on the synergistic regulatory mechanisms between micro-topographical heterogeneity and intercropping systems, offering theoretical support for mitigating soil degradation and optimizing management strategies in terraced tea agroecosystems.
Keyword ：
high-throughput sequencing high-throughput sequencing microbial community microbial community micro-topography micro-topography tea plantation tea plantation
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| GB/T 7714 | Li, Yangxin , Sun, Le , Zhang, Jialin et al. Tea Plant/Ophiopogon japonicus Intercropping Drives the Reshaping of Soil Microbial Communities in Terraced Tea Plantation's Micro-Topographical Units [J]. | AGRICULTURE-BASEL , 2025 , 15 (11) . |
| MLA | Li, Yangxin et al. "Tea Plant/Ophiopogon japonicus Intercropping Drives the Reshaping of Soil Microbial Communities in Terraced Tea Plantation's Micro-Topographical Units" . | AGRICULTURE-BASEL 15 . 11 (2025) . |
| APA | Li, Yangxin , Sun, Le , Zhang, Jialin , Zhao, Hongxue , Su, Tejia , Li, Wenhui et al. Tea Plant/Ophiopogon japonicus Intercropping Drives the Reshaping of Soil Microbial Communities in Terraced Tea Plantation's Micro-Topographical Units . | AGRICULTURE-BASEL , 2025 , 15 (11) . |
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The Microbiome Protocols eBook (MPB) serves as a crucial bridge, filling gaps in microbiome protocols for both wet experiments and data analysis. The first edition, launched in 2020, featured 152 meticulously curated protocols, garnering widespread acclaim. We now extend a sincere invitation to researchers to participate in the upcoming 2nd version of MPB, contributing their valuable protocols to advance microbiome research. image
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| GB/T 7714 | Gao, Yunyun , Peng, Kai , Bai, Defeng et al. The Microbiome Protocols eBook initiative: Building a bridge to microbiome research [J]. | IMETA , 2024 , 3 (2) . |
| MLA | Gao, Yunyun et al. "The Microbiome Protocols eBook initiative: Building a bridge to microbiome research" . | IMETA 3 . 2 (2024) . |
| APA | Gao, Yunyun , Peng, Kai , Bai, Defeng , Bai, Xiao-Ye , Bi, Yujing , Chen, Anqi et al. The Microbiome Protocols eBook initiative: Building a bridge to microbiome research . | IMETA , 2024 , 3 (2) . |
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Introduction: In mountainous tea plantations, which are the primary mode of tea cultivation in China, issues such as soil erosion and declining soil fertility are particularly severe. Although green manure cover is an effective agricultural measure for restoring soil fertility, its application in mountainous tea plantations has been relatively understudied. Methods: This study investigated the effects of continuous green manure cover using the slope-protecting plant Ophiopogon japonicus on tea plant growth and soil microbial community structure. We implemented three treatments: 1 year of green manure coverage, 2 years of coverage, and a control, to study their effects on tea plant growth, soil physicochemical properties, and soil bacterial and fungal communities. Results: Results demonstrate that green manure coverage significantly promote the growth of tea plants, enhanced organic matter and pH levels in soil, and various enzyme activities, including peroxidases and cellulases. Further functional prediction results indicate that green manure coverage markedly promoted several carbon cycling functions in soil microbes, including xylanolysis, cellulolysis, degradation of aromatic compounds, and saprotrophic processes. LEfSe analysis indicated that under green manure cover, the soil tends to enrich more beneficial microbial communities with degradation functions, such as Sphingomonas, Sinomonas, and Haliangium (bacteria), and Penicillium, Apiotrichum, and Talaromyce (fungi). In addition. Random forest and structural equation models indicated that carbon cycling, as a significant differentiating factor, has a significant promoting effect on tea plant growth. Discussion: In the management practices of mountainous tea plantations, further utilizing slope-protecting plants as green manure can significantly influence the soil microbial community structure and function, enriching microbes involved in the degradation of organic matter and aromatic compounds, thereby positively impacting tea tree growth and soil nutrient levels.
Keyword ：
green manure green manure high-throughput sequencing high-throughput sequencing Ophiopogon japonicus Ophiopogon japonicus soil microbiota soil microbiota tea plant tea plant
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| GB/T 7714 | Shao, Shuaibo , Li, Zhongwei , Zhu, Yanqi et al. Green manure (Ophiopogon japonicus) cover promotes tea plant growth by regulating soil carbon cycling [J]. | FRONTIERS IN MICROBIOLOGY , 2024 , 15 . |
| MLA | Shao, Shuaibo et al. "Green manure (Ophiopogon japonicus) cover promotes tea plant growth by regulating soil carbon cycling" . | FRONTIERS IN MICROBIOLOGY 15 (2024) . |
| APA | Shao, Shuaibo , Li, Zhongwei , Zhu, Yanqi , Li, Yi , Li, Yuanping , Wu, Linkun et al. Green manure (Ophiopogon japonicus) cover promotes tea plant growth by regulating soil carbon cycling . | FRONTIERS IN MICROBIOLOGY , 2024 , 15 . |
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本实用新型公开了一种粉碎搅拌一体机，包括设备主体，所述设备主体的内部上方转动配合有破碎辊，所述破碎辊的外部固定连接有若干破碎刀，所述设备主体的顶部两侧均固定连接有第一电机，所述第一电机的驱动端和破碎辊固定连接；所述设备主体的内部左右两端均固定连接有弧形块，所述弧形块之间转动配合有流速控制辊，所述弧形块的底端和设备主体之间转动连接有转轴。通过上述结构，通过第一电机进行驱动，驱动后使其破碎刀进行高速转动，转动后将其对物料实现破碎操作，而流速控制辊的转速可通过第三电机驱动速度控制，将粉碎后的物料匀速流通至设备主体的底端，实现搅拌操作，降低传统直接倾倒，造成搅拌不均匀问题。
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| GB/T 7714 | 蒋政 , 田舒杰 , 黄旭敏 et al. 一种粉碎搅拌一体机 : CN202321684935.7[P]. | 2023-06-30 . |
| MLA | 蒋政 et al. "一种粉碎搅拌一体机" : CN202321684935.7. | 2023-06-30 . |
| APA | 蒋政 , 田舒杰 , 黄旭敏 , 曹世江 , 吴林坤 . 一种粉碎搅拌一体机 : CN202321684935.7. | 2023-06-30 . |
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| GB/T 7714 | Wang, Yao , Hou, Huiyu , Luo, Hao et al. iMeta Conference 2024: Building an innovative scientific research ecosystem for microbiome and One Health [J]. | IMETA , 2024 , 3 (6) . |
| MLA | Wang, Yao et al. "iMeta Conference 2024: Building an innovative scientific research ecosystem for microbiome and One Health" . | IMETA 3 . 6 (2024) . |
| APA | Wang, Yao , Hou, Huiyu , Luo, Hao , Xun, Jiani , Ma, Chuang , Yang, Haifei et al. iMeta Conference 2024: Building an innovative scientific research ecosystem for microbiome and One Health . | IMETA , 2024 , 3 (6) . |
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本实用新型公开了一种胶囊包装结构，包括第一防护壳，所述第一防护壳的下端固定连接有安装座，所述安装座的内部贯穿开设有开口，所述第一防护壳的一端对应设置有第二防护壳，所述第二防护壳的内部设置有安装槽，所述安装槽与安装座相互对应，所述安装座的两端设置有凸块，通过设置安装槽、安装座能够使得两个防护壳进行快速安装连接，从而能够对内部的物件进行防护作用，同时设置凸块、弹簧以及凹槽能够实现两个防护壳之间的卡接作用，确保整个胶囊组件在使用时的稳定性，解决了现行物流产业所产生的大量废弃物、污染物的问题，并进一步提高物流机械化水平、自动化水平，大大提高物流速度。
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| GB/T 7714 | 蒋政 , 严智慧 , 王凌欣 et al. 一种胶囊包装结构 : CN202321684940.8[P]. | 2023-06-30 . |
| MLA | 蒋政 et al. "一种胶囊包装结构" : CN202321684940.8. | 2023-06-30 . |
| APA | 蒋政 , 严智慧 , 王凌欣 , 邱芊 , 曹世江 , 吴林坤 . 一种胶囊包装结构 : CN202321684940.8. | 2023-06-30 . |
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Consecutive monoculture of Rehmannia glutinosa results in severe replant disease and remarkably reduces the yield and quality. Rhizosphere microbiome plays a crucial role in soil health and plant performance. In this study, high-throughput pyrosequencing combined with a culture-dependent approach were applied to analyze the changes in rhizosphere microbiome structure and bacteria-fungi inter-kingdom associations under R. glutinosa consecutive monoculture. The results revealed a distinct separation between the newly planted (NP) and the two-year monocultured (SM). R. glutinosa consecutive monoculture resulted in a significant decline in the Shannon and Simpson diversity indices for the fungal community, and a significant decrease in relative abundances of the phyla Actinobacteria, Chloroflexi and Basidiomycota. The relative abundances of the genera Streptomyces, Arthrobacter, Bacillus, Nocardioides and Lysobacter were significantly higher in NP than in SM, while the opposite was true for Fusarium. Quantitative PCR confirmed a significant reduction in the phylum Actinobacteria and the genus Bacillus, and a significant increase in Fusarium oxysporum. Furthermore, the abundances of Actinobacteria and Bacillus strains with antagonistic activities against Fusarium oxysporum were lower in extended monoculture soils. Most of the isolated antagonistic bacteria were found to be positive for different plant growth promotion activities such as solubilization of phosphate and potassium, production of siderophore and indole-3-acetic acid. In addition, it was found that phenolic acid mixture and catalpol, identified in the root exudates of R. glutinosa, could selectively inhibit or stimulate different microbes. Moreover, the culture filtrate and volatile organic compounds produced by F. oxysporum could in turn suppress the growth of certain antagonistic bacteria. In conclusion, these findings highlight the close association between R. glutinosa replant disease and rhizosphere microbiome assembly, which was mediated by root exudates and microbe-microbe interactions. © 2023, The Authors. All rights reserved.
Keyword ：
Bacteria Bacteria Bacteriology Bacteriology Fungi Fungi Organic acids Organic acids Polymerase chain reaction Polymerase chain reaction Soils Soils Volatile organic compounds Volatile organic compounds
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| GB/T 7714 | Li, Qian , Cai, Sisi , Fang, Yuan et al. Rhizosphere Microbiome Assembly Mediated by Consecutive Monoculture Triggers the Replant Disease of Rehmannia Glutinosa [J]. | SSRN , 2023 . |
| MLA | Li, Qian et al. "Rhizosphere Microbiome Assembly Mediated by Consecutive Monoculture Triggers the Replant Disease of Rehmannia Glutinosa" . | SSRN (2023) . |
| APA | Li, Qian , Cai, Sisi , Fang, Yuan , Khan, Muhammad Umar , Duan, Xuzhong , Fan, Shutong et al. Rhizosphere Microbiome Assembly Mediated by Consecutive Monoculture Triggers the Replant Disease of Rehmannia Glutinosa . | SSRN , 2023 . |
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