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学者姓名：叶文雨
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Abstract ：
Chinese pine, Pinus tabuliformis, is one of the most important garden plants in northern China, and the planting of this species is of great significance for the improvement of the ecological environment. In this study, different fungi were isolated and purified from diseased Pinus tabuliformis samples collected in Xi'an city, Shaanxi Province. Of these fungal isolates, only one (isolate AP-3) was pathogenic to the healthy host plant. The pathogenic isolate was identified as Fusarium acuminatum by morphological characteristics and ITS and TEF-1 alpha sequence analyses. The optimal growth conditions for this isolate were further analyzed as follows: Optimal temperature of 25 degrees C, pH of 11, soluble starch and sodium nitrate as the most preferred carbon and nitrogen sources, respectively. By combining Oxford Nanopore Technologies (ONT) long-read sequencing with Illumina short-read sequencing technologies, we obtained a 41.50 Mb genome assembly for AP-3, with 47.97% GC content and 3.04% repeats. This consisted of 14 contigs with an N50 of 4.64 Mb and a maximum length of 6.45 Mb. The BUSCO completeness of the genome assembly was 98.94% at the fungal level and 97.83% at the Ascomycota level. The genome assembly contained 13,408 protein-coding genes, including 421 carbohydrate-active enzymes (CAZys), 120 cytochrome P450 enzymes (CYPs), 3185 pathogen-host interaction (PHI) genes, and 694 candidate secreted proteins. To our knowledge, this is the first report of F. acuminatum causing needle blight of P. tabuliformis. This study not only uncovered the pathogen responsible for needle blight of P. tabuliformis, but also provided a systematic analysis of its biological characteristics. These findings provide an important theoretical basis for disease control in P. tabuliformis and pave the way for further research into the fungal pathogenicity mechanisms and management strategies.
Keyword ：
biological characteristics biological characteristics Chinese pine Chinese pine fungal isolation fungal isolation genome genome pathogen identification pathogen identification phytopathogenic fungi phytopathogenic fungi
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| GB/T 7714 | Song, Linin , Xu, Yuying , Liu, Tianjin et al. Biological and Genomic Insights into Fusarium acuminatum Causing Needle Blight in Pinus tabuliformis [J]. | JOURNAL OF FUNGI , 2025 , 11 (9) . |
| MLA | Song, Linin et al. "Biological and Genomic Insights into Fusarium acuminatum Causing Needle Blight in Pinus tabuliformis" . | JOURNAL OF FUNGI 11 . 9 (2025) . |
| APA | Song, Linin , Xu, Yuying , Liu, Tianjin , Wang, He , Wang, Xinyue , Fu, Changxiao et al. Biological and Genomic Insights into Fusarium acuminatum Causing Needle Blight in Pinus tabuliformis . | JOURNAL OF FUNGI , 2025 , 11 (9) . |
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本试验从山茶中筛选出一株具有解硅酸盐和有机磷能力的内生细菌，命名为XJX-3,并对其进行了形态学、16S rRNA分子鉴定、菌株生理生化性质研究，还探究了菌株对生菜营养品质及叶绿素含量的影响。结果显示，菌株XJX-3为解淀粉芽孢杆菌，具有淀粉水解、产接触酶、固氮等多种性能。生菜营养品质及叶绿素含量检测结果表明，菌液XJX-3灌根对生菜的维生素C、蛋白质、可溶性糖含量、叶绿素总量较对照均有所提高。本试验可为山茶的合理种植以及解淀粉芽孢杆菌的农业应用提供参考。
Keyword ：
内生细菌 内生细菌 山茶 山茶 生理生化性质 生理生化性质 解淀粉芽孢杆菌 解淀粉芽孢杆菌
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| GB/T 7714 | 肖扬 , 王贺 , 王欣玥 et al. 一株山茶叶片内生细菌的分离及生物学性质研究 [J]. | 青海农林科技 , 2025 , 6 (02) : 71-76 . |
| MLA | 肖扬 et al. "一株山茶叶片内生细菌的分离及生物学性质研究" . | 青海农林科技 6 . 02 (2025) : 71-76 . |
| APA | 肖扬 , 王贺 , 王欣玥 , 陈帅 , 李维波 , 段如意 et al. 一株山茶叶片内生细菌的分离及生物学性质研究 . | 青海农林科技 , 2025 , 6 (02) , 71-76 . |
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Plant lectins have a significant impact on the defense against pathogens and insect attacks. The jacalin-related lectin OsMbl1 from rice (Oryza sativa L.) has been reported to play a crucial role in pattern-triggered immunity (PTI). However, the underlying mechanism remains unclear. In this study, we identified a GDSL-like lipase, OsGdsl1, that interacts with OsMbl1 both in vitro and in vivo. The OsGdsl1 protein, which has lipase activity, is localized in the lipid bodies and apoplast. The expression of OsGDSL1 is modulated upon exposure to Magnaporthe oryzae (M. oryzae) or plant hormones. Deletion of the OsGDSL1 gene not only improved the resistance of rice to M. oryzae, but also led to an increased ROS burst after chitin treatments. The expression of some pathogenesis-related (PR) genes was upregulated in the mutants. We also found that OsMbl1 inhibited the lipase activity of OsGdsl1 during infection with M. oryzae. Overall, our results suggest that OsGdsl1 negatively regulates rice immunity to M. oryzae infection by downregulating ROS bursts and PR gene expressions, while its lipase activity, which is inhibited by OsMbl1, contributes to the enhancement of rice innate immunity during M. oryzae infection.
Keyword ：
lipase lipase Magnaporthe oryzae Magnaporthe oryzae OsMbl1 OsMbl1 plant immunity plant immunity rice rice
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| GB/T 7714 | Song, Linlin , Yang, Tao , Abubakar, Yakubu Saddeeq et al. OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) : 5650-5663 . |
| MLA | Song, Linlin et al. "OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity" . | PLANT CELL AND ENVIRONMENT 48 . 8 (2025) : 5650-5663 . |
| APA | Song, Linlin , Yang, Tao , Abubakar, Yakubu Saddeeq , Han, Yijuan , Zhang, Ruina , Li, Ya et al. OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) , 5650-5663 . |
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Strawberry root rot caused by Neopestalotiopsis clavispora is one of the main diseases of strawberries and significantly impacts the yield and quality of strawberry fruit. Currently, the only accessible control methods are fungicide sprays, which could have an adverse effect on the consumers of the strawberries. Biological control is becoming an alternative method for the control of plant diseases to replace or decrease the application of traditional synthetic chemical fungicides. Trichoderma spp. are frequently used as biological agents to prevent root rot in strawberries. In order to provide highly effective biocontrol resources for controlling strawberry root rot caused by Neopestalotiopsis clavispora, the biocontrol mechanism, the control effects of T. asperellum CMT10 against strawberry root rot, and the growth-promoting effects on strawberry seedlings were investigated using plate culture, microscopy observation, and root drenching methods. The results showed that CMT10 had obvious competitive, antimycotic, and hyperparasitic effects on N. clavispora CMGF3. The CMT10 could quickly occupy nutritional space, and the inhibition rate of CMT10 against CMGF3 was 65.49% 7 d after co-culture. The inhibition rates of volatile metabolites and fermentation metabolites produced by CMT10 were 79.67% and 69.84% against CMGF3, respectively. The mycelium of CMT10 can act as a hyperparasite by contacting, winding, and penetrating the hyphae of CMGF3. Pot experiment showed that the biocontrol efficiency of CMT10 on strawberry root rot caused by Neopestalotiopsis clavispora was 63.09%. CMT10 promoted strawberry growth, plant height, root length, total fresh weight, root fresh weight, stem fresh weight, and root dry weight by 20.09%, 22.39%, 87.11%, 101.58%, 79.82%, and 72.33%, respectively. Overall, this study showed the ability of T. asperellum CMT10 to control strawberry root rot and its potential to be developed as a novel biocontrol agent to replace chemical fungicides for eco-friendly and sustainable agriculture.
Keyword ：
biocontrol efficacy biocontrol efficacy biocontrol mechanism biocontrol mechanism growth-promoting effect growth-promoting effect strawberry root rot strawberry root rot Trichoderma asperellum Trichoderma asperellum
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| GB/T 7714 | Liu, Ping , Yang, Ruixian , Wang, Zuhua et al. Biocontrol Potential of Trichoderma asperellum CMT10 against Strawberry Root Rot Disease [J]. | HORTICULTURAE , 2024 , 10 (3) . |
| MLA | Liu, Ping et al. "Biocontrol Potential of Trichoderma asperellum CMT10 against Strawberry Root Rot Disease" . | HORTICULTURAE 10 . 3 (2024) . |
| APA | Liu, Ping , Yang, Ruixian , Wang, Zuhua , Ma, Yinhao , Ren, Weiguang , Wei, Daowei et al. Biocontrol Potential of Trichoderma asperellum CMT10 against Strawberry Root Rot Disease . | HORTICULTURAE , 2024 , 10 (3) . |
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Rice blast, caused by the fungal pathogen Magnaporthe oryzae (M. oryzae), is a highly destructive disease that significantly impacts rice yield and quality. During the infection, M. oryzae secretes effector proteins to subvert the host immune response. However, the interaction between the effector protein AvrPik-D and its target proteins in rice, and the mechanism by which AvrPik-D exacerbates disease severity to facilitate infection, remains poorly understood. In this study, we found that the M. oryzae effector AvrPik-D interacts with the Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) small subunit OsRBCS4. The overexpression of the OsRBCS4 gene in transgenic rice not only enhances resistance to M. oryzae but also induces more reactive oxygen species following chitin treatment. OsRBCS4 localizes to chloroplasts and co-localizes with AvrPik-D within these organelles. AvrPik-D suppresses the transcriptional expression of OsRBCS4 and inhibits Rubisco activity in rice. In conclusion, our results demonstrate that the M. oryzae effector AvrPik-D targets the Rubisco small subunit OsRBCS4 and inhibits its carboxylase and oxygenase activity, thereby suppressing rice innate immunity to facilitate infection. This provides a novel mechanism for the M. oryzae effector to subvert the host immunity to promote infection.
Keyword ：
AvrPik-D AvrPik-D Magnaporthe oryzae Magnaporthe oryzae OsRBCS4 OsRBCS4 resistance resistance rice rice Rubisco Rubisco
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| GB/T 7714 | Song, Linlin , Yang, Tao , Wang, Xinxiao et al. Magnaporthe oryzae Effector AvrPik-D Targets Rice Rubisco Small Subunit OsRBCS4 to Suppress Immunity [J]. | PLANTS-BASEL , 2024 , 13 (9) . |
| MLA | Song, Linlin et al. "Magnaporthe oryzae Effector AvrPik-D Targets Rice Rubisco Small Subunit OsRBCS4 to Suppress Immunity" . | PLANTS-BASEL 13 . 9 (2024) . |
| APA | Song, Linlin , Yang, Tao , Wang, Xinxiao , Ye, Wenyu , Lu, Guodong . Magnaporthe oryzae Effector AvrPik-D Targets Rice Rubisco Small Subunit OsRBCS4 to Suppress Immunity . | PLANTS-BASEL , 2024 , 13 (9) . |
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Botrytis cinerea is the causal agent of gray mold disease affecting Paeoniaceae plants. The present study focuses on the potential of Bacillus amyloliquefaciens Mdgb15 as a promising biological control agent toward B. cinerea. The strain Mdgb15 showed the highest inhibition rate (55.56%) among all the strains tested against B. cinerea growth and was effective in controlling tree peony gray mold in detached leaves. A conventional PCR with three specific primer pairs detected lipopetides substance synthesis genes of Mdgb15. The results revealed that Mdgb15 had srfAA, fenA and ituD genes, which resulted in the production of surfactin, fengycin and iturin. An accurate qRT-PCR protocol with specifically designed primers was developed to characterize the expression of lipopeptide synthesis gene of Mdgb15. The qRT-PCR analysis revealed that the expression of the ituD and fenA genes in Mdgb15 was significantly up-regulated during the coculture with B. cinerea. Subsequently, the lipopeptides were extracted by acid precipitation of culture filtrate of Mdgb15 and were separated by Sephadex Gel chromatography. Three separated fractions exhibited strong antifungal activity against B. cinerea, and were identified as bacillomycin D by MALDI-TOF-MS analysis. This study has demonstrated that B. amyloliquefaciens Mdgb15 is a future potential new biocontrol agent for controlling tree peony gray mold disease. It suggests that the antifungal mechanisms of Mdgb15 may be at least partially associated with its ability to secret bacillomycin D.
Keyword ：
Antifungal activity Antifungal activity Bacillomycin D Bacillomycin D Bacillus amyloliquefaciens Bacillus amyloliquefaciens Botrytis cinerea Botrytis cinerea Lipopeptides Lipopeptides Paeonia suffruticosa Paeonia suffruticosa
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| GB/T 7714 | Yang, Ruixian , Ye, Wenyu , Liu, Ping et al. Endophytic Bacillus amyloliquefaciens Mdgb15 is a potential biocontrol agent against tree peony gray mold caused by Botrytis cinerea [J]. | EUROPEAN JOURNAL OF PLANT PATHOLOGY , 2024 , 169 (2) : 431-445 . |
| MLA | Yang, Ruixian et al. "Endophytic Bacillus amyloliquefaciens Mdgb15 is a potential biocontrol agent against tree peony gray mold caused by Botrytis cinerea" . | EUROPEAN JOURNAL OF PLANT PATHOLOGY 169 . 2 (2024) : 431-445 . |
| APA | Yang, Ruixian , Ye, Wenyu , Liu, Ping , Li, Juan , Lu, Mingmei , Wang, Zuhua et al. Endophytic Bacillus amyloliquefaciens Mdgb15 is a potential biocontrol agent against tree peony gray mold caused by Botrytis cinerea . | EUROPEAN JOURNAL OF PLANT PATHOLOGY , 2024 , 169 (2) , 431-445 . |
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分离鉴定柚子炭疽病的病原菌，对其致病性和生物学特性进行研究。利用组织分离法对柚子炭疽病的病原菌进行分离纯化；利用离体叶片接种方法检测其致病性；通过形态学观察和ITS基因序列同源性比对及其系统进化分析确定其分类地位；利用单因素试验研究其生物学特性。结果表明，分离得到的致病真菌YZ4被鉴定为胶孢炭疽菌(Colletotrichum gloeosporioides);最适合YZ4生长的碳源为淀粉，氮源为蛋白胨，最适生长pH为5.0。研究结果为柚子炭疽病的防治提供基础数据。
Keyword ：
叶片病害 叶片病害 柚子 柚子 炭疽病 炭疽病 生物学特性 生物学特性
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| GB/T 7714 | 陈帅 , 李维波 , 陈诗瑜 et al. 一株柚子叶部病原真菌的鉴定及生物学特性研究 [J]. | 青海农林科技 , 2024 , 6 (01) : 7-11,41 . |
| MLA | 陈帅 et al. "一株柚子叶部病原真菌的鉴定及生物学特性研究" . | 青海农林科技 6 . 01 (2024) : 7-11,41 . |
| APA | 陈帅 , 李维波 , 陈诗瑜 , 肖扬 , 刘天金 , 张春源 et al. 一株柚子叶部病原真菌的鉴定及生物学特性研究 . | 青海农林科技 , 2024 , 6 (01) , 7-11,41 . |
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本研究采用组织分离法从鸡蛋花(Plumeria rubra Linn.cv.Acutifolia)叶片中分离出一株病原真菌菌株，命名为JD3,并对菌落形态特征和ITS序列分析。结果显示，鉴定JD3菌株为胶孢炭疽菌(Colletotrichum gloeosporioides),该菌株能够利用多种碳源和氮源，其中D-甘露糖醇为最佳碳源，酸水解酪蛋白为最佳氮源。本研究结果可为诊断和防治鸡蛋花炭疽病提供参考。
Keyword ：
炭疽菌 炭疽菌 生物学特性 生物学特性 真菌鉴定 真菌鉴定 鸡蛋花 鸡蛋花
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| GB/T 7714 | 李维波 , 陈帅 , 张春源 et al. 一株鸡蛋花叶部病原真菌的鉴定及生物学特性研究 [J]. | 青海农林科技 , 2024 , 7 (02) : 8-14 . |
| MLA | 李维波 et al. "一株鸡蛋花叶部病原真菌的鉴定及生物学特性研究" . | 青海农林科技 7 . 02 (2024) : 8-14 . |
| APA | 李维波 , 陈帅 , 张春源 , 赖泽成 , 叶文雨 . 一株鸡蛋花叶部病原真菌的鉴定及生物学特性研究 . | 青海农林科技 , 2024 , 7 (02) , 8-14 . |
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Strawberry root rot caused by Fusarium solani is one of the main diseases of strawberries and significantly impacts the yield and quality of strawberry fruit. Biological control is becoming an alternative method for the control of plant diseases to replace or decrease the application of traditional chemical fungicides. To obtain antagonistic bacteria with a high biocontrol effect on strawberry root rot, over 72 rhizosphere bacteria were isolated from the strawberry rhizosphere soil and screened for their antifungal activity against F. solani by dual culture assay. Among them, strains CMS5 and CMR12 showed the strongest inhibitory activity against F. solani (inhibition rate 57.78% and 65.93%, respectively) and exhibited broad-spectrum antifungal activity. According to the phylogenetic tree based on 16S rDNA and gyrB genes, CMS5 and CMR12 were identified as Bacillus amyloliquefaciens. Lipopeptide genes involved in surfactin, iturin, and fengycin biosynthesis were detected in the DNA genomes of CMS5 and CMR12 by PCR amplification. The genes related to the three major lipopeptide metabolites existed in the DNA genome of strains CMS5 and CMR12, and the lipopeptides could inhibit the mycelial growth of F. solani and resulted in distorted hyphae. The inhibitory rates of lipopeptides of CMS5 and CMR12 on the spore germination of F. solani were 61.00% and 42.67%, respectively. The plant-growth-promoting (PGP) traits in vitro screening showed that CMS5 and CMR12 have the ability to fix nitrogen and secreted indoleacetic acid (IAA). In the potting test, the control efficiency of CMS5, CMR12 and CMS5+CMR12 against strawberry root rot were 65.3%, 67.94% and 88.00%, respectively. Furthermore, CMS5 and CMR12 enhanced the resistance of strawberry to F. solani by increasing the activities of defense enzymes MDA, CAT and SOD. Moreover, CMS5 and CMR12 significantly promoted the growth of strawberry seedlings such as root length, seedling length and seedling fresh weight. This study revealed that B. amyloliquefaciens CMS5 and CMR12 have high potential to be used as biocontrol agents to control strawberry root rot.
Keyword ：
antifungal lipopeptides antifungal lipopeptides Bacillus amyloliquefaciens Bacillus amyloliquefaciens biological control biological control Fragariaxananassa Fragariaxananassa Fusarium solani Fusarium solani growth-promoting effect growth-promoting effect
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| GB/T 7714 | Yang, Ruixian , Liu, Ping , Ye, Wenyu et al. Biological Control of Root Rot of Strawberry by Bacillus amyloliquefaciens Strains CMS5 and CMR12 [J]. | JOURNAL OF FUNGI , 2024 , 10 (6) . |
| MLA | Yang, Ruixian et al. "Biological Control of Root Rot of Strawberry by Bacillus amyloliquefaciens Strains CMS5 and CMR12" . | JOURNAL OF FUNGI 10 . 6 (2024) . |
| APA | Yang, Ruixian , Liu, Ping , Ye, Wenyu , Chen, Yuquan , Wei, Daowei , Qiao, Cuicui et al. Biological Control of Root Rot of Strawberry by Bacillus amyloliquefaciens Strains CMS5 and CMR12 . | JOURNAL OF FUNGI , 2024 , 10 (6) . |
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The Chinese flowering cherry (Cerasus serrulata), an ornamental tree with established medicinal values, is observed to suffer from leaf blight within Xi'an's greenbelts. This disease threatens both the plant's growth and its ornamental appeal. In this study, 26 isolates were obtained from plants with typical leaf blight, and only 3 isolates (XA-10, XA-15, and XA-18) were found to be pathogenic, causing similar symptoms on the leaves of the host plant. Based on sequence alignment, the ITS and LSU sequences of the three selected isolates were consistent, respectively. Following morphological and molecular analyses, the three selected isolates were further identified as Mortierella alpina. The three selected isolates exhibited similar morphological characteristics, including wavy colonies with dense, milky-white aerial mycelia on PDA medium. Therefore, isolate XA-10 was used as a representative strain for subsequent experiments. The representative strain XA-10 was found to exhibit optimal growth at a temperature of 30(degrees)C and a pH of 7.0. Host range infection tests further revealed that the representative strain XA-10 could also inflict comparable disease symptoms on both the leaves and fruits of three different Rosaceae species (Prunus persica, Pyrus bretschneideri, and Prunus salicina). This study reveals, for the first time, the causative agent of leaf blight disease affecting the Chinese flowering cherry. This provides a deeper understanding of the biology and etiology of M. alpina. This study lays a solid foundation for the sustainable control and management of leaf blight disease in the Chinese flowering cherry.
Keyword ：
biological characteristics biological characteristics Cerasus serrulata Cerasus serrulata Mortierella alpina Mortierella alpina pathogenic fungi pathogenic fungi pathogenicity pathogenicity
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| GB/T 7714 | Shao, Dengke , Xu, Yuying , Zhang, Chunyuan et al. Identification and Biological Characteristics of Mortierella alpina Associated with Chinese Flowering Cherry (Cerasus serrulata) Leaf Blight in China [J]. | JOURNAL OF FUNGI , 2024 , 10 (1) . |
| MLA | Shao, Dengke et al. "Identification and Biological Characteristics of Mortierella alpina Associated with Chinese Flowering Cherry (Cerasus serrulata) Leaf Blight in China" . | JOURNAL OF FUNGI 10 . 1 (2024) . |
| APA | Shao, Dengke , Xu, Yuying , Zhang, Chunyuan , Lai, Zecheng , Song, Linlin , Su, Jiyu et al. Identification and Biological Characteristics of Mortierella alpina Associated with Chinese Flowering Cherry (Cerasus serrulata) Leaf Blight in China . | JOURNAL OF FUNGI , 2024 , 10 (1) . |
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