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学者姓名:刘泓
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Cadmium (Cd) inputs in soils have been significantly increased over the last 20 years. Various approaches have been used to remove Cd from contaminated soils, with bioremediation through plants and soil microbes being the most effective. This study investigated interactions of a new plant growth promoting fungus (PGPF), Cladosporium 'BF-F' with Sesuvium portulacastrum plants for bioremediation of Cd in a laboratory setting. Results showed that 'BF-F' was able to colonize S. portulacastrum which substantially increased plant growth and Cd accumulation in S. portulacastrum. To gain potential mechanisms underlying the 'BF-F'-mediated Cd accumulation, colonized plants along with control plants were further investigated via transcriptomic analysis. Data indicated that the expression of genes related to zinc and copper uptake and transport were significantly higher in Cdstressed plants inoculated with 'BF-F'. The upregulation of these genes promoted plant uptake of Cd, but reduced uptake of zinc and copper. Therefore, 'BF-F' can increase the biomass and the expression of metal ion transport genes to enhance the absorption of Cd by S. portulacastrum, and promote Cd phytoremediation. This study provides a novel insight for understanding of Cladosporium 'BF-F' to improve phytoremediation of Cd, which shows potential applications in bioremediation of Cd from contaminated soils.
Keyword :
Cd absorption and transport Cd absorption and transport Cladosporium sp.'BF-F' Cladosporium sp.'BF-F' Metal ion transport Metal ion transport S. portulacastrum S. portulacastrum Transcriptome Transcriptome
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| GB/T 7714 | Zhang, Wenbin , Cao, Yanyu , Ma, Mengnan et al. Plant growth promoting fungus Cladosporium 'BF-F' promotes cadmium accumulation in Sesuvium portulacastrum by regulating plant metal ion transport [J]. | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 2025 , 300 . |
| MLA | Zhang, Wenbin et al. "Plant growth promoting fungus Cladosporium 'BF-F' promotes cadmium accumulation in Sesuvium portulacastrum by regulating plant metal ion transport" . | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 300 (2025) . |
| APA | Zhang, Wenbin , Cao, Yanyu , Ma, Mengnan , Li, Ziling , Jiang, Xiuli , Wang, Dan et al. Plant growth promoting fungus Cladosporium 'BF-F' promotes cadmium accumulation in Sesuvium portulacastrum by regulating plant metal ion transport . | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 2025 , 300 . |
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为探究有机物料与功能菌联合施用对植烟土壤微生物群落的影响,采用大田试验,在常规有机肥中添加不同有机物料及功能菌,研究了它们施用后植烟土壤微生物群落的状况。试验结果表明:有机物料及固氮、溶磷、解钾菌的施用会影响土壤微生物群落的结构及组成,猪粪配方与功能菌联合施用对促进土壤氮循环尤为显著,可加快土壤的氮循环速率,对土壤磷、钾有效性提升也有促进作用,烟草旺长期烟叶氮、磷、钾含量分别提升了13%、40%、25%。牛粪及碳化谷壳配方在增加土壤腐生真菌的数量方面有明显优势,有助于促进有机物分解。
Keyword :
功能菌 功能菌 微生物群落 微生物群落 有机物料 有机物料 植烟土壤 植烟土壤
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| GB/T 7714 | 赵安丽 , 蒋桂花 , 卢思宇 et al. 有机物料与功能菌联合施用对植烟土壤微生物群落的影响 [J]. | 中国烟草科学 , 2025 , 46 (03) : 45-54 . |
| MLA | 赵安丽 et al. "有机物料与功能菌联合施用对植烟土壤微生物群落的影响" . | 中国烟草科学 46 . 03 (2025) : 45-54 . |
| APA | 赵安丽 , 蒋桂花 , 卢思宇 , 卢敏娇 , 郭吴晔 , 魏欣琪 et al. 有机物料与功能菌联合施用对植烟土壤微生物群落的影响 . | 中国烟草科学 , 2025 , 46 (03) , 45-54 . |
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Excess antimony (Sb) has been shown to damage plant growth. Rice plants readily absorb a large amount of Sb after a long period of flooding, yet the mechanisms underlying Sb toxicity in plants have not been solved. This study was conducted to explore the effects of Sb on the uptake of N and S, and monitor the concentrations of reduced glutathione (GSH) and enzymes associated with these processes. In addition, we analyzed differentially expressed metabolites (DEMs) correlated with amino acids (AAs) and oligopeptides, specifically DEMs containing sulfur (S), GSH and indole-3-acetic acid (IAA). The results showed that antimonite [Sb(III)] inhibited shoot growth whereas antimonate [Sb(V)] stimulated shoot growth. Interestingly, Sb(III)5/10 enhanced shoot concentrations of total nitrogen (N), NH4+-N [only at Sb(III)10] and S; but reduced the shoot concentrations of NO3-N and soluble protein. Sb(III)5/10 addition significantly increased oxidized glutathione (GSSG) concentration and activities of glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST) but non-significantly affected concentration of reduced glutathione (GSH) and activities of gamma-glutamylcysteine synthetase (GCL) and glutathione reductase (GR), suggesting Sb(III) restricted GSH recycling. Addition of Sb (1) increased the abundance of DEMs associated with lignins, Ca uptake, toxicity/detoxification, and branched chain AAs; (2) decreased the abundance of AAs inclcuding isoleucine (Ile), leucine (Leu), tryptophan (Trp), tyrosine (Tyr) and histidine (His); (3) increased the abundance of arginine (Arg), putrescine (Put) and spermidine (Spd); and (4) affected methylation and acetylation of many AAs, especially acetylation.
Keyword :
Amino acids Amino acids Antimonate Antimonate Antimonite Antimonite Glutathione derivates Glutathione derivates Metabolomics Metabolomics
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| GB/T 7714 | Chen, Qiaoyuan , Zhu, Yanming , Zhang, Jiajia et al. Toxicity of antimony to plants: Effects on metabolism of N and S in a rice plant [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2024 , 216 . |
| MLA | Chen, Qiaoyuan et al. "Toxicity of antimony to plants: Effects on metabolism of N and S in a rice plant" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 216 (2024) . |
| APA | Chen, Qiaoyuan , Zhu, Yanming , Zhang, Jiajia , Tong, Yiran , Liu, Hong , Rensing, Christopher et al. Toxicity of antimony to plants: Effects on metabolism of N and S in a rice plant . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2024 , 216 . |
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Different forms of antimony (Sb) show different toxicities to plants, which are hypothesized to be partially due to the disorders of lipid and saccharide synthesis. Hydroponic experiments were conducted using a rice plant (Yangdao 6) exposed to antimonite (Sb(III)) and antimonate (Sb(V)). We monitored the following (1) saccharide concentration and enzymatic activities associated with synthesis/degradation of sucrose and starch; (2) changes in cell ultrastructure of rice leaves; and (3) differentially expressed metabolites (DEMs) associated with lipids. The results showed that when compared to the control, Sb(III/V) (1) increased the concentrations of starch, soluble sugars, sucrose and fructose as well as the activities of cell-wall binding acid invertase (B-AI) in rice leaves; (2) mainly affected the abundance of unsaturated lipids of fatty acids (FAs), prenol lipids, glycerolipids, and glycerophospholipids, especially for Sb(III); and (3) negatively affected the abundance of DEMs associated with alpha-linolenic acid metabolism and xanthophyll formation. Relative to Sb(V), Sb(III) (1) showed great negative effects on the activities of fructose-1, 6-diphosphatase (FBP), triose-phosphate isomerase (TPI), alpha-glucosidase, and sucrose-phosphate synthase (SPS); (2) significantly narrowed the shape of starch granules and increased the thickness of cell walls; (3) increased numbers and abundance of DEMs associated with toxins (belonging to sphingolipids), flavonoids (polyketides), and biomarkers (corticosteroid hormones); and (4) increased the numbers of FAs whose abundance was upregulated. This study showed a complex regulatory network associated with saccharide synthesis/degradation and lipid constitution in response to Sb toxicity.
Keyword :
Antimony Antimony Cell membranes Cell membranes Lipids Lipids Soluble sugars Soluble sugars Starch Starch
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| GB/T 7714 | Zheng, Yating , Zhu, Yanming , Tong, Yiran et al. Effects of antimony on synthesis of saccharides and lipids, and enzyme activity associated with synthesis/degradation of saccharides in leaves of a rice plant [J]. | PLANT STRESS , 2024 , 15 . |
| MLA | Zheng, Yating et al. "Effects of antimony on synthesis of saccharides and lipids, and enzyme activity associated with synthesis/degradation of saccharides in leaves of a rice plant" . | PLANT STRESS 15 (2024) . |
| APA | Zheng, Yating , Zhu, Yanming , Tong, Yiran , Zhang, Jiajia , Liu, Hong , Rensing, Christopher et al. Effects of antimony on synthesis of saccharides and lipids, and enzyme activity associated with synthesis/degradation of saccharides in leaves of a rice plant . | PLANT STRESS , 2024 , 15 . |
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Selenium (Se) is a microelement that can counteract (a)biotic stresses in plants. Excess antimony (Sb) will inhibit plant photosynthesis, which can be alleviated by appropriate doses of Se but the associated mechanisms at the molecular levels have not been fully explored. Here, a rice variety (Yongyou 9) was exposed to selenite [Se(IV), 0.2 and 0.8 mg L-1] alone or combined with antimonite [Sb(III), 5 and 10 mg L-1]. When compared to the 10 mg L-1 Sb treatment alone, addition of Se in a dose-dependent manner 1) reduced the heat dissipation efficiency resulting from the inhibited donors, Sb concentrations in shoots and roots, leaf concentrations of fructose, H2O2 and O2 center dot-; 2) enhanced heat dissipation efficiency resulting from the inhibited accepters value, concentrations of Chl a, sucrose and starch, and the enzyme activity of adenosine diphosphate glucose pyrophosphorylase, sucrose phosphate synthase, and sucrose synthase; but 3) did not alter gas exchange parameters, concentrations of Chl b and total Chl, enzyme activity of soluble acid invertase, and values of maximum P700 signal, photochemical efficiency of PSI and electron transport rate of PSI. Se alleviated the damage caused by Sb to the oxygen-evolving complex and promoted the transfer of electrons from QA to QB. When compared to the 10 mg L-1 Sb treatment alone, addition of Se 1) up-regulated genes correlated to synthesis pathways of Chl, carotenoid, sucrose and glucose; 2) disturbed signal transduction pathway of abscisic acid; and 3) upregulated gene expression correlated to photosynthetic complexes (OsFd1, OsFER1 and OsFER2).
Keyword :
Carbon assimilation and metabolism Carbon assimilation and metabolism Chlorophyll fluorescence Chlorophyll fluorescence Gas exchange parameters Gas exchange parameters Photosynthetic system Photosynthetic system Transcriptome Transcriptome
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| GB/T 7714 | Zhao, Pingping , Wu, ZiHan , Zheng, YaTing et al. Selenite affected photosynthesis of Oryza sativa L. exposed to antimonite: Electron transfer, carbon fixation, pigment synthesis via a combined analysis of physiology and transcriptome [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2023 , 201 . |
| MLA | Zhao, Pingping et al. "Selenite affected photosynthesis of Oryza sativa L. exposed to antimonite: Electron transfer, carbon fixation, pigment synthesis via a combined analysis of physiology and transcriptome" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 201 (2023) . |
| APA | Zhao, Pingping , Wu, ZiHan , Zheng, YaTing , Shen, Jun , Zhu, YanMing , Chen, QiaoYuan et al. Selenite affected photosynthesis of Oryza sativa L. exposed to antimonite: Electron transfer, carbon fixation, pigment synthesis via a combined analysis of physiology and transcriptome . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2023 , 201 . |
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Ecological restoration of heavily contaminated soils by multiple metal(loid)s in mining areas is very difficult. In this study, we provided an attractive measure of using silkworm excrement (SE) and its modified materials to restore the soil heavily contaminated by arsenic (As), antimony (Sb), cadmium (Cd), lead (Pb) and chromium (Cr). We investigated the adsorption capacities and the associated remediation mechanisms for antimonite [Sb (III)] and antimonate [Sb(V)] by raw SE, biochar-modified SE (BC700), iron-modified BC700 (MBC) and sulfhydryl-modified BC700 (SH). Then, we selected SE and SH to compare their outcomes to restore the vegetations and the soil bacterial communities in the investigated soil mentioned above. The results showed that SE displayed the best characteristics for metal(loid) physical adsorption. But SH conferred the strongest capacity to adsorb Sb (max 23.92 mg g-1), suggesting the process of chemical adsorption played a key role in adsorbing Sb via functional groups (-SH). SE and SH both significantly (1) promoted the growth of pakchoi (Brassica campestris L., New Zealand No.2), community abundance of soil bacteria (283-936 OTUs), and the quantity of bacterial genera correlated with resistance, plant growth promotion and specified carbon metabolism; (2) but reduced
Keyword :
Antimony Antimony Biochar Biochar Ecological restoration Ecological restoration Silkworm excrement Silkworm excrement Sulfhydryl modification Sulfhydryl modification
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| GB/T 7714 | Shi, Shengjie , Yang, Jigang , Lin, Mengting et al. Using silkworm excrement to restore vegetation and soil ecology in heavily contaminated mining soils by multiple metal(loid)s: A recyclable sericulture measure [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 459 . |
| MLA | Shi, Shengjie et al. "Using silkworm excrement to restore vegetation and soil ecology in heavily contaminated mining soils by multiple metal(loid)s: A recyclable sericulture measure" . | JOURNAL OF HAZARDOUS MATERIALS 459 (2023) . |
| APA | Shi, Shengjie , Yang, Jigang , Lin, Mengting , Chen, Qiaoyuan , Wang, Bo , Zhao, Jiayi et al. Using silkworm excrement to restore vegetation and soil ecology in heavily contaminated mining soils by multiple metal(loid)s: A recyclable sericulture measure . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 459 . |
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香蕉枯萎病菌热带4号生理小种(Fusarium oxysporum f. sp.cubense tropical race 4,Foc TR4)严重威胁世界香蕉产业的可持续发展,一些特殊功能的蛋白在其侵染过程中起重要作用。我们分析Foc TR4接种巴西蕉后的转录组数据发现M35金属蛋白酶(metalloprotease 35)同源基因FocM35_2在病原菌侵染初期受诱导显著上调表达,经同源重组法敲除该基因,突变体致病力显著下降,菌丝生长速率降低,产孢量略微减少,回补突变体则可以恢复其致病力和生长发育;与野生型菌株相比较,突变体对外源氧胁迫、渗透压和细胞膜压力更敏感;在本氏烟草(Nicotiana benthamiana)中的瞬时表达结果显示该蛋白定位于质外体,并引起叶片产生超敏反应(HR,hypersensitive response);该蛋白的缺失还能够诱导寄主更高水平几丁质酶的活性。因此,FocM35_2是促进Foc TR4侵染寄主的的重要致病因子。
Keyword :
基因敲除 基因敲除 细胞坏死 细胞坏死 致病性 致病性 金属蛋白酶 金属蛋白酶 香蕉枯萎病菌 香蕉枯萎病菌
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| GB/T 7714 | 吴邦庭 , 刘思文 , 张晓霞 et al. 金属蛋白酶FocM35_2在香蕉枯萎病菌热带4号生理小种侵染过程中的功能 [J]. | 植物病理学报 , 2023 , 53 (01) : 41-51 . |
| MLA | 吴邦庭 et al. "金属蛋白酶FocM35_2在香蕉枯萎病菌热带4号生理小种侵染过程中的功能" . | 植物病理学报 53 . 01 (2023) : 41-51 . |
| APA | 吴邦庭 , 刘思文 , 张晓霞 , 李春雨 , 刘泓 . 金属蛋白酶FocM35_2在香蕉枯萎病菌热带4号生理小种侵染过程中的功能 . | 植物病理学报 , 2023 , 53 (01) , 41-51 . |
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Previously studies have demonstrated that selenium (Se) can reduce cadmium (Cd) concentration in plants. However, its underlying mechanisms on relationships among Cd uptake/translocation, plant root growth, essential elemental uptake, cell wall components (CWCs), and cell wall enzymes were not fully elucidated. In this study, a hydroponic system was set up to explore the above relationships in a pakchoi variety (Brassica rapa L., LvYou 102) exposed to solo- or co- exposure of Cd (0.5 mg L-1) and Se (0.5 mg L-1) using a combined analysis of physiology and transcriptome. The results showed that Se reduced shoot Cd concentration and its translocation from roots to shoots. Se significantly stimulated root growth and root concentrations of potassium and magne-sium, but reduced shoot concentrations of all tested essential elements as compared to the Cd treatment alone. Se significantly enhanced galacturonic acid (GalA) and total sugar concentrations in pectins but reduced that in hemicellulose I (HCI); while Se significantly increased GalA concentration but reduced total sugar concentration in hemicellulose II (HCII) when compared to the Cd treatment alone. Se enhanced activities of pectinesterase (PME), beta-galactosidase (beta-Gal), and xyloglucan endoglycosidase/hydrolase (XTH), but did not alter that of polygalacturonase (PG) and cellulase (CE) when compared to the Cd treatment alone. Under Cd exposure, Se in most cases down-regulated genes correlated with elemental uptake, degradation of pectins and hemicelluloses, and synthesis of cellulose and lignins, except for some up-regulated genes including XTH27, XTH22 and BraA10g 021100.3 C.
Keyword :
Cell wall enzymes Cell wall enzymes Gene regulation Gene regulation Hemicelluloses Hemicelluloses Pectin demethylation Pectin demethylation Potassium regulation Potassium regulation
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| GB/T 7714 | Liu, ZiQing , Wu, ZiHan , Chen, QiaoYuan et al. Selenite inhibited cadmium translocation and stimulated root growth of Brassica rapa L.: Regulation of element uptake, polysaccharide synthesis and crosslink, and cell wall enzymes [J]. | ENVIRONMENTAL AND EXPERIMENTAL BOTANY , 2023 , 211 . |
| MLA | Liu, ZiQing et al. "Selenite inhibited cadmium translocation and stimulated root growth of Brassica rapa L.: Regulation of element uptake, polysaccharide synthesis and crosslink, and cell wall enzymes" . | ENVIRONMENTAL AND EXPERIMENTAL BOTANY 211 (2023) . |
| APA | Liu, ZiQing , Wu, ZiHan , Chen, QiaoYuan , Lin, MengTing , Zheng, YaTing , Liu, Yang et al. Selenite inhibited cadmium translocation and stimulated root growth of Brassica rapa L.: Regulation of element uptake, polysaccharide synthesis and crosslink, and cell wall enzymes . | ENVIRONMENTAL AND EXPERIMENTAL BOTANY , 2023 , 211 . |
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Selenium (Se) can reduce cadmium (Cd) uptake/translocation via regulating pectins, hemicelluloses and lignins of plant root cell walls, but the detailed molecular mechanisms are not clear. In this study, six hydroponic ex-periments were set up to explore the relationships of uptake/translocation inhibition of Cd by selenite (Se(IV)) with cell wall component (CWC) synthesis and/or interactions. Cd and Se was supplied (alone or combinedly) at 1.0 mg L-1 and 0.5 mg L-1, respectively, with the treatment without Cd and Se as the control. When compared to the Cd1 treatment, the Se0.5Cd1 treatment 1) significantly increased total sugar concentrations in pectins, hemicelluloses and callose, suggesting an enhanced capacity of binding Cd or blocking Cd translocation; 2) stimulated the deposition of Casparian strips (CS) in root endodermis and exodermis to block Cd translocation; 3) stimulated the release of C-O-C (-OH- or -O-) and C--O (carboxyl, carbonyl, or amide) to combine Cd; 4) regulated differential expression genes (DEGs) and metabolites (DMs) correlated with synthesis and/or in-teractions of CWSs to affect cell wall net structure to affect root cell division, subsequent root morphology and finally elemental uptake; and 5) stimulated de-methylesterification of pectins via reducing expression abun-dances of many DMs and DEGs in the Yang Cycle to reduce supply of methyls to homogalacturonan, and regulated gene expressions of pectin methylesterase to release carboxyls to combine Cd; and 6) down-regulated gene expressions associated with Cd uptake/translocation.
Keyword :
Arabinoxylan-pectin-arabinogalactan-proteins Arabinoxylan-pectin-arabinogalactan-proteins Lignin Lignin Root cell wall Root cell wall structure structure Suberin Suberin translocation inhibition translocation inhibition Uptake Uptake
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| GB/T 7714 | Wang, LiZhen , Wu, KongYuan , Liu, ZiQing et al. Selenite reduced uptake/translocation of cadmium via regulation of assembles and interactions of pectins, hemicelluloses, lignins, callose and Casparian strips in rice roots [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 448 . |
| MLA | Wang, LiZhen et al. "Selenite reduced uptake/translocation of cadmium via regulation of assembles and interactions of pectins, hemicelluloses, lignins, callose and Casparian strips in rice roots" . | JOURNAL OF HAZARDOUS MATERIALS 448 (2023) . |
| APA | Wang, LiZhen , Wu, KongYuan , Liu, ZiQing , Li, ZengFei , Shen, Jun , Wu, ZiHan et al. Selenite reduced uptake/translocation of cadmium via regulation of assembles and interactions of pectins, hemicelluloses, lignins, callose and Casparian strips in rice roots . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 448 . |
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Potassium (K+) is one of the most important macronutrients for plant growth and development. It is generally accepted that the KUP/HAK/KT transporters play essential roles in K+ uptake at low concentrations. However, their physiological functions in bananas remain unknown. Here, we cloned MaHAK5 and analyzed its functions in banana (Musa acuminata). Gene expression analysis showed that MaHAK5 was upregulated in the roots and leaves in the early stage of low K+ (LK) stress. MaHAK5 was localized in the cytomembrane. The expression of MaHAK5 improved the growth of the low K+-sensitive yeast mutant R5421 at different K+ supply levels. Overexpression of MaHAK5 in Arabidopsis thaliana significantly enhanced the ability for K+ uptake and increased the chlorophyll content under LK stress. These results indicate that MaHAK5 plays a crucial role in maintaining K+ uptake in bananas.
Keyword :
banana banana K+ uptake K+ uptake low K+ stress low K+ stress MaHAK5 MaHAK5 Potassium transporter Potassium transporter
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| GB/T 7714 | Wu, Bangting , Xie, Yanling , Xiang, Dandan et al. MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic Arabidopsis under Low Potassium Conditions [J]. | HORTICULTURAE , 2023 , 9 (1) . |
| MLA | Wu, Bangting et al. "MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic Arabidopsis under Low Potassium Conditions" . | HORTICULTURAE 9 . 1 (2023) . |
| APA | Wu, Bangting , Xie, Yanling , Xiang, Dandan , Yi, Ganjun , Liu, Hong , Li, Chunyu et al. MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic Arabidopsis under Low Potassium Conditions . | HORTICULTURAE , 2023 , 9 (1) . |
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