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学者姓名：许卫锋
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Abstract ：
Heat stress significantly impacts global rice production, highlighting the critical need to understand the genetic basis of heat resistance in rice. U2AF (U2 snRNP auxiliary factor) is an essential splicing complex with critical roles in recognizing the 3 '-splice site of precursor messenger RNAs (pre-mRNAs). The U2AF small subunit (U2AF35) can bind to the 3 '-AG intron border and promote U2 snRNP binding to the branch-point sequences of introns through interaction with the U2AF large subunit (U2AF65). However, the functions of U2AF35 in plants are poorly understood. In this study, we discovered that the OsU2AF35a gene was vigorously induced by heat stress and could positively regulate rice thermotolerance during both the seedling and reproductive growth stages. OsU2AF35a interacts with OsU2AF65a within the nucleus, and both of them can form condensates through liquid-liquid phase separation (LLPS) following heat stress. The intrinsically disordered regions (IDR) are accountable for their LLPS. OsU2AF35a condensation is indispensable for thermotolerance. RNA-seq analysis disclosed that, subsequent to heat treatment, the expression levels of several genes associated with water deficiency and oxidative stress in osu2af35a-1 were markedly lower than those in ZH11. In accordance with this, OsU2AF35a is capable of positively regulating the oxidative stress resistance of rice. The pre-mRNAs of a considerable number of genes in the osu2af35a-1 mutant exhibited defective splicing, among which was the OsHSA32 gene. Knocking out OsHSA32 significantly reduced the thermotolerance of rice, while overexpressing OsHSA32 could partially rescue the heat sensitivity of osu2af35a-1. Together, our findings uncovered the essential role of OsU2AF35a in rice heat stress response through protein separation and regulating alternative pre-mRNA splicing.
Keyword ：
condensation condensation liquid-liquid phase separation liquid-liquid phase separation OsHSA32 OsHSA32 OsU2AF35a OsU2AF35a pre-mRNA splicing pre-mRNA splicing rice thermotolerance rice thermotolerance
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| GB/T 7714 | Liu, Jianping , Li, Xin , Wang, Ke et al. The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre-mRNA in rice [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (4) : 1308-1328 . |
| MLA | Liu, Jianping et al. "The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre-mRNA in rice" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 4 (2025) : 1308-1328 . |
| APA | Liu, Jianping , Li, Xin , Wang, Ke , Wang, Tao , Meng, Yang , Peng, Zhi et al. The splicing auxiliary factor OsU2AF35a enhances thermotolerance via protein separation and promoting proper splicing of OsHSA32 pre-mRNA in rice . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (4) , 1308-1328 . |
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Understanding the regulatory mechanisms underlying the plant heat stress response is important for developing climate-resilient crops, including rice (Oryza sativa). Here, we report that OsEIL5, one member of the ETHYLENE INSENSITIVE3-LIKE family, positively regulates rice heat tolerance at the seedling and reproductive stages. OsEIL5 directly binds to the promoter of OsPP91 (encoding one type 2C protein phosphatase) and activates its expression. OsPP91 is required for rice thermotolerance, and overexpressing OsPP91 in oseil5-1 partially rescues its heat sensitivity. The F box protein OsEBF1 interacts with OsEIL5 and degrades it through the ubiquitination pathway, resulting in the reduction of OsPP91 expression and ultimately weakening rice heat tolerance. Knocking out OsEIL5 in the EBF1R13 line partially reduces its extremely high heat tolerance. Taken together, our work uncovers a mechanism that finely regulates rice thermotolerance through the OsEBF1-OsEIL5-OsPP91 module at the posttranslational and transcriptional levels.
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| GB/T 7714 | Liu, Jianping , Wang, Ke , Wang, Guining et al. The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation [J]. | CELL REPORTS , 2025 , 44 (2) . |
| MLA | Liu, Jianping et al. "The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation" . | CELL REPORTS 44 . 2 (2025) . |
| APA | Liu, Jianping , Wang, Ke , Wang, Guining , Peng, Zhi , Wang, Tao , Meng, Yang et al. The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation . | CELL REPORTS , 2025 , 44 (2) . |
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White lupin (Lupinus albus L.) produces cluster roots to acquire more phosphorus under phosphorus deficiency. Bacillus amyloliquefaciens SQR9 contributes to plant growth, but whether and how it promotes cluster root formation in white lupin remain unclear. Here, we investigated the roles of SQR9 in cluster root formation under low phosphorus conditions using a microbial mutant and virus-induced gene silencing (VIGS) in white lupin. SQR9 substantially enhanced cluster root formation under low phosphorus conditions. The ysnE gene encodes an auxin biosynthesis enzyme in SQR9 and was associated with cluster root formation, as ysnE-defective SQR9 did not trigger cluster root formation. SQR9 inoculation induced the expression of PIN-formed2 (LaPIN2, encoding an auxin transporter) and YUCCA4 (LaYUC4, encoding an auxin biosynthesis enzyme) in white lupin roots. VIGS-mediated knockdown of LaPIN2 and LaYUC4 prevented wild-type SQR9-induced cluster root formation in white lupin. Finally, white lupin LaYUC4-derived auxin and SQR9-derived auxin pools were both transported by LaPIN2, promoting cluster root formation under low phosphorus conditions. Taken together, we propose that B. amyloliquefaciens promotes cluster root formation in white lupin under low phosphorus conditions by stimulating auxin biosynthesis and transport. Our results provide insights into the interplay between bacteria and root auxin in crop phosphorus use efficiency. Bacillus amyloliquefaciens produces auxin and promotes auxin biosynthesis in white lupin under low phosphorus conditions, triggering cluster root formation.
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| GB/T 7714 | Yang, Jinyong , Li, Shenglan , Zhou, Xiangxue et al. Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels [J]. | PLANT PHYSIOLOGY , 2025 , 197 (2) . |
| MLA | Yang, Jinyong et al. "Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels" . | PLANT PHYSIOLOGY 197 . 2 (2025) . |
| APA | Yang, Jinyong , Li, Shenglan , Zhou, Xiangxue , Du, Chongxuan , Fang, Ju , Li, Xing et al. Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels . | PLANT PHYSIOLOGY , 2025 , 197 (2) . |
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本发明公开一个白羽扇豆排根生长关键基因LaLBD29及其应用。所述LaLBD29基因的核苷酸序列如SEQ ID NO.1所示，其编码的蛋白的氨基酸序列如SEQ ID NO.2所示。本发明以野生型白羽扇豆为背景材料，利用转基因技术证实了沉默LaLBD29基因会抑制缺磷条件下白羽扇豆排根形成和耐缺磷胁迫能力。本发明为耐缺磷作物品种的选育和遗传改良提供了重要理论和实践依据。
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| GB/T 7714 | 许卫锋 , 王正瑞 , 刘建平 et al. 一个白羽扇豆排根生长关键基因LaLBD29及其应用 : CN202510101500.2[P]. | 2025-01-22 . |
| MLA | 许卫锋 et al. "一个白羽扇豆排根生长关键基因LaLBD29及其应用" : CN202510101500.2. | 2025-01-22 . |
| APA | 许卫锋 , 王正瑞 , 刘建平 , 许飞云 , 杨金勇 . 一个白羽扇豆排根生长关键基因LaLBD29及其应用 : CN202510101500.2. | 2025-01-22 . |
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White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.
Keyword ：
cluster root formation cluster root formation DNA methylation DNA methylation melatonin synthesis melatonin synthesis phosphorus deficiency phosphorus deficiency white lupin white lupin
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| GB/T 7714 | Zhang, Qian , Li, Xing , Geng-Li, Jiahong et al. DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) : 8548-8560 . |
| MLA | Zhang, Qian et al. "DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis" . | PLANT CELL AND ENVIRONMENT 48 . 12 (2025) : 8548-8560 . |
| APA | Zhang, Qian , Li, Xing , Geng-Li, Jiahong , Yang, Jinyong , Liu, Jingyi , Wang, Ke et al. DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) , 8548-8560 . |
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本发明公开了超表达OsCHS1基因在促进水稻生长、提高水稻磷吸收和提高水稻耐低磷性中的应用。通过将OsCHS1基因转入水稻野生型（WT）植株，获得水稻超表达OsCHS1植株。在对照和减低磷件下，水稻超表达OsCHS1植株与WT相比，OsCHS1基因表达量、株高、地上部鲜重、根系鲜重、地上部干重、根系干重、总根长和地上部磷浓度都显著增加。因此可以通过超表达OsCHS1基因的方法提高促进水稻生长和水稻磷吸收，为磷高效利用和水稻高产打下基础。
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| GB/T 7714 | 许飞云 , 李亮 , 许卫锋 et al. 超表达OsCHS1基因在促进水稻生长、提高水稻磷吸收和提高水稻耐低磷性中的应用 : CN202510368429.4[P]. | 2025-03-27 . |
| MLA | 许飞云 et al. "超表达OsCHS1基因在促进水稻生长、提高水稻磷吸收和提高水稻耐低磷性中的应用" : CN202510368429.4. | 2025-03-27 . |
| APA | 许飞云 , 李亮 , 许卫锋 , 丁凡 , 刘建平 . 超表达OsCHS1基因在促进水稻生长、提高水稻磷吸收和提高水稻耐低磷性中的应用 : CN202510368429.4. | 2025-03-27 . |
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本发明提供了一种水稻溶磷菌及其应用，属于生物菌剂技术领域。本发明所述菌株：水稻溶磷菌Acinetobactersp.X2，可使水稻发芽率提高31.8％‑38.1％；处理间水稻株高、根干重、总根长、地上部干重和地上部鲜重均存在显著差异。在提高水稻的产量等方面有广阔的应用前景，且有助于稳定或提高水稻产量，保障粮食安全。
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| GB/T 7714 | 许飞云 , 许卫锋 , 李亮 et al. 一种水稻溶磷菌及其应用 : CN202410703333.4[P]. | 2024-06-03 . |
| MLA | 许飞云 et al. "一种水稻溶磷菌及其应用" : CN202410703333.4. | 2024-06-03 . |
| APA | 许飞云 , 许卫锋 , 李亮 , 刘建平 , 徐梦强 . 一种水稻溶磷菌及其应用 : CN202410703333.4. | 2024-06-03 . |
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本发明属于微生物技术领域，具体涉及一株过表达iaam基因的硝基还原假单胞菌工程菌及其构建方法与应用。所述构建方法为：以硝基还原假单胞菌LSF‑6基因组DNA为模板，对硝基还原假单胞菌LSF‑6基因组DNA中的iaam基因进行PCR扩增；将PCR扩增产物连接到pBBR1MCS载体，构建iaam基因过表达载体；将iaam基因过表达载体转化到硝基还原假单胞菌LSF‑6中，获得过表达iaam基因的硝基还原假单胞菌工程菌。所述硝基还原假单胞菌工程菌较出发菌株的植物生长素分泌能力明显提高。将所述硝基还原假单胞菌工程菌制备成菌悬液施用于水稻，可以使水稻的株高、根长、地上部鲜重、地下部鲜重和叶绿素SPAD值明显增加。本发明为微生物法促进水稻生长奠定了基础。
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| GB/T 7714 | 许卫锋 , 王泳森 , 杨金勇 et al. 一种过表达iaam基因的硝基还原假单胞菌工程菌及其构建方法与应用 : CN202411704950.2[P]. | 2024-11-26 . |
| MLA | 许卫锋 et al. "一种过表达iaam基因的硝基还原假单胞菌工程菌及其构建方法与应用" : CN202411704950.2. | 2024-11-26 . |
| APA | 许卫锋 , 王泳森 , 杨金勇 , 许飞云 , 刘建平 , 徐梦强 et al. 一种过表达iaam基因的硝基还原假单胞菌工程菌及其构建方法与应用 : CN202411704950.2. | 2024-11-26 . |
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本发明提供了一种耐酸促生菌高地芽孢杆菌及在作物种子包衣中的应用，属于微生物技术领域。本发明提供的耐酸促生菌高地芽孢杆菌FAFU1及其微生物包衣制剂，能够有效提升水稻在酸性胁迫环境中的生理适应性。在酸性红壤条件下，经菌株接种或微生物包衣处理的水稻植株，其株高、地上部干重、根系生物量及总根长等关键生长指标均呈现显著提升。该菌株及制备得到的包衣产品为酸性土壤农作系统的微生物修复提供了创新性技术支撑，对提高边际土地作物生产力具有重要应用价值。
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| GB/T 7714 | 许飞云 , 许卫锋 , 丁凡 et al. 一种耐酸促生菌高地芽孢杆菌及在作物种子包衣中的应用 : CN202510430124.1[P]. | 2025-06-06 . |
| MLA | 许飞云 et al. "一种耐酸促生菌高地芽孢杆菌及在作物种子包衣中的应用" : CN202510430124.1. | 2025-06-06 . |
| APA | 许飞云 , 许卫锋 , 丁凡 , 李亮 , 刘建平 . 一种耐酸促生菌高地芽孢杆菌及在作物种子包衣中的应用 : CN202510430124.1. | 2025-06-06 . |
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Intracellular Ca2+ controls various cellular functions and local Ca2+ dynamics are tightly regulated upon environmental cues. Maintaining cellular Ca2+ balance is essential for plant survival. Here we report a calcium-dependent protein kinases (CPKs)-mediated signaling pathway, in conjunction with the ER membrane-resident Ca2+-ATPase ECA1, acts influentially for cytosolic Ca2+ homeostasis and osmotic stress tolerance. We show that targeting cytosolic Ca2+ efflux via specific inhibitors or eca1 mutation results in augmented [Ca2+]cyt spikes, elevated cytoplasmic ABA ([ABA]cyt) level and ultimately hypersensitive to osmotic stress. Screening of Arabidopsis CPKs revealed direct binding of CPK2/6/11 to ECA1. Moreover, CPK2/6/11 phosphorylates the N-terminal of ECA1 at Ser5, thereby enhancing its activity for cytosolic Ca2+ efflux into ER and subsequently lowering [ABA]cyt. The cumulative effect of ECA1 and CPKs mutation on Arabidopsis plant sensitivity to osmotic stress further illustrates that CPKs/ECA1 acts as an intracellular module for tuning stress response via regulating [Ca2+]cyt and [ABA]cyt homeostasis. © 2025, The Authors. All rights reserved.
Keyword ：
Cell signaling Cell signaling Plant diseases Plant diseases
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| GB/T 7714 | Liang, Xiaoju , Zhou, Yeling , Xu, Weifeng et al. An Intracellular Calcium-Associated Module CPKs/ECA1 Fine-Tunes Cytosolic Calcium and ABA Homeostasis for Plant Osmosensitivity [J]. | SSRN , 2025 . |
| MLA | Liang, Xiaoju et al. "An Intracellular Calcium-Associated Module CPKs/ECA1 Fine-Tunes Cytosolic Calcium and ABA Homeostasis for Plant Osmosensitivity" . | SSRN (2025) . |
| APA | Liang, Xiaoju , Zhou, Yeling , Xu, Weifeng , Liang, Jiansheng . An Intracellular Calcium-Associated Module CPKs/ECA1 Fine-Tunes Cytosolic Calcium and ABA Homeostasis for Plant Osmosensitivity . | SSRN , 2025 . |
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