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学者姓名:CHRISTOPHER GUENTHER THEODOR RENSING

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Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation SCIE
期刊论文 | 2026 , 288 | WATER RESEARCH
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Microbial biofilm-based hydrovoltaic electricity generators (BioHEGs) have recently been developed as promising and readily available platforms for green energy harvesting, despite their unsatisfactory output performances and unspecified mechanisms regarding electric current production. Herein, carbon quantum dots (CQDs) were used to construct a nano-biohybrid system with Shewanella oneidensis MR-1 (S. oneidensis), through which the CQDs/S. oneidensis BioHEG achieved a maximum open-circuit voltage of ca. 0.65 V and short-circuit current density of ca. 5.23 mu A & sdot;cm-2. In addition, both the hydrovoltaic effect and electrical conductivity of CQDs/S. oneidensis nano-biohybrids were noticeably improved due to enhanced secretion of extracellular polymeric substances (EPS) and accelerated electron transfer upon CQDs implantation, thereby leading to a nearly 14-fold increase in output power density compared to the bare S. oneidensis cells. Studies aimed to elucidate the underlying mechanism indicated that the hybridization of CQDs and S. oneidensis greatly promoted the metabolic synthesis of outer membrane c-type cytochromes (OM c-Cyts) and the extracellular secretion of riboflavin (RF), which was demonstrated to be decisive in the current producing process of the CQDs/ S. oneidensis BioHEG. This work thus proposes a viable strategy to boost the hydrovoltaic electricity generation capacity of microbial biofilms and provides a new perspective on the mechanism of accelerated electron transfer pathways inside BioHEGs.

Keyword :

Carbon quantum dots Carbon quantum dots Hydrovoltaic electricity generation Hydrovoltaic electricity generation Metabolic synthesis Metabolic synthesis Shewanella oneidensis Shewanella oneidensis

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GB/T 7714 Chen, Ting-Ting , Yan, Zhi-Wu , Cai, Feng-Ying et al. Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation [J]. | WATER RESEARCH , 2026 , 288 .
MLA Chen, Ting-Ting et al. "Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation" . | WATER RESEARCH 288 (2026) .
APA Chen, Ting-Ting , Yan, Zhi-Wu , Cai, Feng-Ying , He, Qiu-Xiang , You, Han-Hui , Rensing, Christopher et al. Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation . | WATER RESEARCH , 2026 , 288 .
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Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential SCIE
期刊论文 | 2025 , 118 (1) | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
WoS CC Cited Count: 2
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Abstract :

Two facultatively aerobic strains, designated SGZ-02(T) and SGZ-792(T), were isolated from plant Pennisetum sp., exhibiting the highest 16S rRNA gene sequence similarities with the type strains of Sphingomonas zeae LMG 28739(T) (98.6%) and Massilia forsythiae NBRC 114511(T) (98.4%), respectively. SGZ-02(T) grew between 5 and 45 degrees C, pH 5.0-11.0 and tolerated NaCl concentrations of 0-4% (w/v), whereas SGZ-792(T) thrived at 5-40 degrees C, pH 5.0-11.0 and NaCl tolerance to 0-3.5% (w/v). The major quinone of SGZ-02(T) was ubiquinone-10, with the dominant fatty acids being C-16:0 (13.5%), Summed Feature 3 (6.3%), C-14:02-OH (5.3%) and Summed Feature 8 (66.3%). SGZ-792(T) predominantly contained ubiquinone-8, with major fatty acids being C-16:0 (20.3%), Summed Feature 3 (5.0%) and Summed Feature 8 (54.7%). Average nucleotide identity and digital DNA-DNA hybridization values between two strains and their closest references strains were below the bacterial species threshold. Based on genotypic and phenotypic characteristics, strains SGZ-02(T) and SGZ-792(T) are proposed as novel species within the genera Sphingomonas and Massilia, respectively. The suggested names for the new species are Sphingomonas fuzhouensis sp. nov. (SGZ-02(T) = GDMCC 1.4033(T) = JCM 36769(T)) and Massilia phyllosphaerae sp. nov. (SGZ-792(T) = GDMCC 1.4211(T) = JCM 36643(T)), respectively.

Keyword :

Massilia phyllosphaerae sp. nov. Massilia phyllosphaerae sp. nov. Plant growth-promoting Plant growth-promoting Polyphasic taxonomy Polyphasic taxonomy Sphingomonas fuzhouensis sp. nov. Sphingomonas fuzhouensis sp. nov.

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GB/T 7714 Yao, Ling , Liu, Guo-Hong , Zhang, Shu-Yi et al. Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential [J]. | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY , 2025 , 118 (1) .
MLA Yao, Ling et al. "Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential" . | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 118 . 1 (2025) .
APA Yao, Ling , Liu, Guo-Hong , Zhang, Shu-Yi , Gao, Peng , Rensing, Christopher , Yang, Qiu-E et al. Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential . | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY , 2025 , 118 (1) .
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Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties SCIE
期刊论文 | 2025 , 512 (1-2) , 819-836 | PLANT AND SOIL
WoS CC Cited Count: 2
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Background and aimsSilicon (Si) has been shown to alleviate cadmium (Cd) toxicity in plants, but the effect of Si on hyperaccumulators such as S. alfredii has not been fully elucidated. This study evaluated the impact of exogenous Si on growth, Cd accumulation, and rhizosphere properties of S. alfredii.MethodsSi was applied to S. alfredii and various parameters were assessed, including biomass, shoot Cd content, chlorophyll, carotenoid levels, malondialdehyde (MDA) content, antioxidant enzyme activity, cell wall composition, soil nutrient availability, and rhizosphere enzyme activities. A thorough transcriptomic analysis was also conducted.ResultsSi significantly increased shoot biomass (21.47-104.35%) and Cd accumulation (144.60-747.11%) in S. alfredii. Si also enhanced chlorophyll levels, reduced the malondialdehyde (MDA) content, increased antioxidant enzyme activities, and mitigated Cd-induced oxidative stress. Si boosted cell wall components, thereby facilitating Cd transport in roots. In rhizosphere soil, Si improved alkaline hydrolysis of nitrogen (AHN), available phosphorus (AP), available potassium (AK), dissolved organic carbon (DOC), and increased available Cd content. Si enhanced rhizospheric enzyme activities. Positive correlations were observed between rhizosphere nutrient availability and plant biomass, enzyme activities and Cd accumulation. Transcriptome analysis showed a higher expression of stress responsive transcription factors (WRKY, basic leucine zipper (bZIP), MYB, NAC) and genes encoding functions involved in metal transport (Heavy Metal ATPases (HMA), Copper Transporter (COPT), chelation (Phytochelatins (PCs), Metallothioneins (MTs), Glutathione (GSH)), and cell wall biosynthesis (Cellulose synthases (Ces), Pectin Methylesterases (PME)).ConclusionsSi enhanced the tolerance and accumulation of Cd in S. alfredii, underscoring its potential in phytoextraction applications.

Keyword :

Antioxidant defense Antioxidant defense Biomass Biomass Phytoextraction Phytoextraction Rhizospheric nutrient Rhizospheric nutrient Transcriptomic analysis Transcriptomic analysis

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GB/T 7714 Xu, Junlong , Shao, Yudie , Rao, Xinhao et al. Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties [J]. | PLANT AND SOIL , 2025 , 512 (1-2) : 819-836 .
MLA Xu, Junlong et al. "Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties" . | PLANT AND SOIL 512 . 1-2 (2025) : 819-836 .
APA Xu, Junlong , Shao, Yudie , Rao, Xinhao , Tang, Jinliang , Rensing, Christopher , Alwathnani, Hend et al. Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties . | PLANT AND SOIL , 2025 , 512 (1-2) , 819-836 .
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Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus SCIE
期刊论文 | 2025 , 513 (2) , 2807-2825 | PLANT AND SOIL
WoS CC Cited Count: 4
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Background and aimsThe long-term monoculture of tea plants leads to the accumulation of soil pathogens, disruption of the ecological balance of soil microbial communities. Intercropping systems, have been shown to enhance soil ecological stability. However, there is still a lack of research on the effects of the tea plant/Ophiopogon japonicus intercropping system on soil nutrient cycling and key microbial communities. MethodsA three-year field experiment was conducted to compare two treatments, monoculture and intercropping. Growth parameters such as tea plant height, canopy width, and bud number were investigated. High-throughput sequencing was used to evaluate the impact of the intercropping system on soil microbial community structure and function. ResultsThe intercropping system significantly promotes tea plant growth, with plant height, bud number, and fresh weight increasing by an average of 10.87%, 24.26%, and 23.53%, respectively. Soil organic matter content increased by an average of 23.83%. Simultaneously, the diversity and symbiotic relationships of soil microbial communities continue to strengthen, with significant enrichment observed in Proteobacteria, and Basidiomycota. Random forest modeling further indicated that intercropping enhanced the abundance of microbial taxa involved in organic matter decomposition, such as Pseudomonas, Sphingomonas, and Archaeorhizomyces, consistent with significant increases in cellulase and polyphenol oxidase activities (P < 0.05). Additionally, the intercropping system significantly reduced the relative abundance of pathogenic fungi such as Cladosporium and Curvularia. ConclusionIn tea cultivation practices, intercropping tea plants with O. japonicus can improve the soil micro-ecology of mountain tea plantations, restore soil vitality, and promote tea plant growth.

Keyword :

High-throughput sequencing High-throughput sequencing Intercropping Intercropping Soil microbiota Soil microbiota Tea plant/Ophiopogon japonicas Tea plant/Ophiopogon japonicas

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GB/T 7714 Shao, Shuaibo , Li, Zhongwei , Ma, Xiaoxiao et al. Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus [J]. | PLANT AND SOIL , 2025 , 513 (2) : 2807-2825 .
MLA Shao, Shuaibo et al. "Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus" . | PLANT AND SOIL 513 . 2 (2025) : 2807-2825 .
APA Shao, Shuaibo , Li, Zhongwei , Ma, Xiaoxiao , Cui, Jingru , Zhu, Yanqi , Li, Yuanping et al. Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus . | PLANT AND SOIL , 2025 , 513 (2) , 2807-2825 .
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Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation SCIE
期刊论文 | 2025 , 14 (1) | PLANTS-BASEL
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As sustainable forest management gains increasing attention, comprehending the impact of stand density on soil properties and microbial communities is crucial for optimizing forest ecosystem functions. This study employed high-throughput sequencing in conjunction with soil physicochemical analysis to assess the effects of stand density on soil physicochemical properties and microbial community characteristics in Chinese fir plantations, aiming to elucidate the influence of density regulation on ecosystem services. Our results suggested that changes in soil physicochemical properties and microenvironmental conditions were key drivers of soil microbial diversity. Total carbon (TC), soluble nitrogen (SN), and light fraction organic matter decreased with increasing stand density, while total potassium (TK) and available phosphorus (AP) concentrations increased. The plot with a density of 900 trees ha-1 exhibited the highest bacterial diversity, in contrast to the plot with 1500 trees ha-1, which showed the lowest. The dominant microbial taxa were similar across different stand retention densities, with Acidobacteria, Proteobacteria, and Chloroflexi being the predominant bacterial phyla and Ascomycota and Basidiomycota being the main fungal groups. Significant positive correlations were observed between soil microbial community structures and environmental factors, particularly with respect to soil phosphorus and nitrogen content. The present study demonstrated that reduced stand densities modulated soil nutrient content and enhanced bacterial diversity, thereby contributing to a more complex and stable soil ecosystem structure. These insights provide a scientific foundation for optimizing the management of Chinese fir plantations, thereby supporting the sustainable development of forest ecosystems.

Keyword :

Chinese fir Chinese fir high-throughput sequencing high-throughput sequencing microbial diversity microbial diversity plantation forest plantation forest soil microorganisms soil microorganisms

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GB/T 7714 Xu, Zuyuan , Fan, Fei , Lin, Qinmin et al. Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation [J]. | PLANTS-BASEL , 2025 , 14 (1) .
MLA Xu, Zuyuan et al. "Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation" . | PLANTS-BASEL 14 . 1 (2025) .
APA Xu, Zuyuan , Fan, Fei , Lin, Qinmin , Guo, Shengzhou , Li, Shumao , Zhang, Yunpeng et al. Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation . | PLANTS-BASEL , 2025 , 14 (1) .
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Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms SCIE
期刊论文 | 2025 , 47 , 194-203 | ENGINEERING
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Phototrophy and chemotrophy are two dominant types of microbial metabolism. However, to date, the potential of the ubiquitous and versatile mechanical energy as a renewable energy source to drive the growth of microorganisms has remained unknown and not utilized. Here, we present evidence in favor of a previously unidentified metabolic pathway, in which the electronic energy produced from mechanical energy by the piezoelectric materials is used to support the growth of microorganisms. When electroactive microorganism Rhodopseudomonas palustris (R. palustris; with barium titanate nanoparticles) was mechanically stirred, a powerful biohybrid piezoelectric effect (BPE) enabled sustainable carbon fixation coupled with nitrate reduction. Transcriptomic analyses demonstrated that mechanical stirring of the bacteria-barium titanate biohybrid led to upregulation of genes encoding functions involved in electron and energy transfer in R. palustris. Studies with other electroactive microorganisms suggested that the ability of microbes to utilize BPE may be a common phenomenon in the microbial world. Taken together, these findings imply a long-neglected and potentially important microbial metabolic pathway, with potential importance to microbial survival in the energy-limited environments. (c) 2024 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Keyword :

Biohybrid piezoelectric effect Biohybrid piezoelectric effect Carbon fixation Carbon fixation Mechanical energy Mechanical energy Microbial metabolism Microbial metabolism Nitrate reduction Nitrate reduction

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GB/T 7714 Ren, Guoping , Ye, Jie , Liu, Lu et al. Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms [J]. | ENGINEERING , 2025 , 47 : 194-203 .
MLA Ren, Guoping et al. "Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms" . | ENGINEERING 47 (2025) : 194-203 .
APA Ren, Guoping , Ye, Jie , Liu, Lu , Hu, Andong , Nealson, Kenneth H. , Rensing, Christopher et al. Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms . | ENGINEERING , 2025 , 47 , 194-203 .
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Early inoculation and bacterial community assembly in plants: A review SCIE
期刊论文 | 2025 , 296 | MICROBIOLOGICAL RESEARCH
WoS CC Cited Count: 1
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The relationship between plants and early colonizing microbes is crucial for regulating agricultural ecosystems. Recent evidence strongly suggests that by introducing beneficial microbes during the seed or seedling stages, the diversity and assembly structure of the plant-related microbial community during later plant development can be altered, recruiting beneficial bacteria to enhance plant protection. However, the mechanisms of community assembly and their effects on plant growth are still not fully understood. To deepen our understanding of the importance of early inoculation for improving plant performance, this review comprehensively summarizes recent research advancements on the effects of early introduction on plant growth and adaptability. The mechanisms and ecological significance of early inoculation in the assembly of plant-related bacterial communities are discussed, with particular emphasis on the importance of seed endophytes, plant growth-promoting rhizobacteria (PGPR), and synthetic microbial consortia as microbial inoculants in enhancing plant health and productivity. Additionally, this review proposes a new strategy: sequential inoculation during the seed and seedling stages, aiming to maximize the effects of microbes.

Keyword :

Agricultural resources Agricultural resources Assembly mechanism Assembly mechanism Early inoculation Early inoculation Priority effect Priority effect

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GB/T 7714 Wang, Xing , Li, Yuyi , Rensing, Christopher et al. Early inoculation and bacterial community assembly in plants: A review [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 296 .
MLA Wang, Xing et al. "Early inoculation and bacterial community assembly in plants: A review" . | MICROBIOLOGICAL RESEARCH 296 (2025) .
APA Wang, Xing , Li, Yuyi , Rensing, Christopher , Zhang, Xiaoxia . Early inoculation and bacterial community assembly in plants: A review . | MICROBIOLOGICAL RESEARCH , 2025 , 296 .
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Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau SCIE
期刊论文 | 2025 , 293 | MICROBIOLOGICAL RESEARCH
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Soil microbes, as intermediaries in plant-soil interactions, are closely linked to plant health in grassland ecosystems. In recent years, varying degrees of degradation have been observed in the alpine grasslands of the Qinghai-Tibet Plateau (QTP). Addressing grassland degradation, particularly under the influence of climate change, poses a global challenge. Understanding the factors driving grassland degradation on the QTP and developing appropriate mitigation measures is essential for the future sustainability of this fragile ecosystem. In this review, we discuss the environmental and anthropogenic factors affecting grassland degradation and the corresponding impacts on soil microbe community structure. We summarize the current research on the microbiome of the QTP, in particular the effect of vegetation, climate change, grazing, and land use, respectively on the alpine grassland microbiome. The results of these studies indicate that microbially mediated soil bioprocesses are important drivers of grassland ecosystem functional recovery. Therefore, a thorough understanding of the spatial distribution characteristics of the soil microbiome in alpine grasslands is required, and this necessitates an integrated approach in which the interactions among climatic factors, vegetation characteristics, and human activities are evaluated. Additionally, we assess and summarise current technological developments and prospects for applying soil microbiome technologies in sustainable agriculture, including: (i) single-strain inoculation, and (ii) inoculation of synthetic microbial communities, (iii) microbial community transplantation. Grassland restoration projects should be carried out with the understanding that each restoration measure has a unique effect on the soil microbial activity. We propose that the sustainable development of alpine grassland ecosystems can be achieved by adopting advanced microbiome technologies and integrating microbebased sustainable agricultural practices to maximise grassland biomass, increase soil carbon, and optimise soil nutrient cycling.

Keyword :

Alpine grassland Alpine grassland Ecosystem functions Ecosystem functions Influencing factors Influencing factors Microbiome techniques Microbiome techniques Soil microbes Soil microbes

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GB/T 7714 Zhang, Mingxu , Hu, Jinpeng , Zhang, Yuewei et al. Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 293 .
MLA Zhang, Mingxu et al. "Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau" . | MICROBIOLOGICAL RESEARCH 293 (2025) .
APA Zhang, Mingxu , Hu, Jinpeng , Zhang, Yuewei , Cao, Yanhua , Rensing, Christopher , Dong, Quanmin et al. Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau . | MICROBIOLOGICAL RESEARCH , 2025 , 293 .
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Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine SSCI
期刊论文 | 2025 , 14 (2) | LAND
WoS CC Cited Count: 1
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Soil metal(loid) pollution is a threat to ecological and environmental safety. The vegetation recovery in mining areas is of great significance for protecting soil resources. In this study, (1) we first gathered four types of soils to analyse their contamination degree, including tailings mud (TM), wasteland soil (TS) very near TM, as well as non-rhizosphere soils of pepper (PF) and maize (MF) in a farmland downstream from the TM (about 5 km). Geo-accumulation and potential ecological risk indices indicated that the soil samples were mainly polluted by antimony (Sb), arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb), and copper (Cu) to different degrees. Leachates of TM resulted in increased Sb, As, and Cd accumulation in TS. (2) Then, we sampled six local plants growing in the TS to assess the possibilities of using these plants as recovery vegetation in TS, of which Persicaria maackiana (Regel) Nakai ex T. Mori absorbed relatively high Sb concentrations in the leaves and roots. (3) After that, we collected rhizosphere soil and tissue samples from eight crops on the above farmland to assess their capacities as recovering vegetation of contaminated farmland soil, of which the fruits of maize accumulated the lowest concentrations of most monitored metal(loid)s (except for Pb). Further, we compared the differences in the bacterial community structure of MF, PF, TM, and TS to assess capacities of cultivating pepper and maize to improve soil microbial community structure. The MF displayed the best characteristics regarding the following attributes: (1) the highest concentrations of OMs and total P; (2) the highest OTU numbers and diversity of bacteria; and (3) the lowest abundance of bacteria with potentially pathogenic and stress-tolerant phenotypes.

Keyword :

mining area mining area multiple-metal(loid) contamination multiple-metal(loid) contamination soil ecological recovery soil ecological recovery soil microorganism soil microorganism

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GB/T 7714 Zhu, Yanming , Yang, Jigang , Zhang, Jiajia et al. Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine [J]. | LAND , 2025 , 14 (2) .
MLA Zhu, Yanming et al. "Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine" . | LAND 14 . 2 (2025) .
APA Zhu, Yanming , Yang, Jigang , Zhang, Jiajia , Tong, Yiran , Su, Hailan , Rensing, Christopher et al. Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine . | LAND , 2025 , 14 (2) .
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Bacterial rrn copy numbers linked to soil function and crop yield in long-term manure-fertilized soils SCIE
期刊论文 | 2025 , 209 | APPLIED SOIL ECOLOGY
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The 16S rRNA operon (rrn) copy number in bacteria has been proposed as a genomic trait linked to microbial lifehistory strategies and resource availability. Yet, its role in agroecosystems under different management histories is unclear. We investigated how soil microbial communities and metabolites respond to 36 years of varying manure fertilization strategies (chemical fertilizers with or without manure) using 16S rRNA gene amplicon sequencing and nontargeted metabolomics. Manure treatments favored copiotrophic bacteria, increasing the average rrn copy numbers while reducing oligotrophs. Microbial life-history strategies were primarily driven by C: N, SOC, and available phosphorus (AP). The rrn copy number was positively correlated with the abundance of genes encoding functions for C, N, and P cycling, as well as correlated enzyme activities, indicating that copiotroph-dominated communities in manure-fertilized soils exhibit a competitor strategy to utilize a broader range of resources. Significant correlations between soil metabolite profiles and rrn copy numbers, and strong interactions between copiotrophic strategists and key metabolites suggest that microbial communities with distinct life-history strategies harbor unique metabolic profiles. PLS-PM modeling and random forest analysis identified rrn copy number as a predictive trait for soil functions (metabolic profiles and enzyme activities) and maize yield. These findings highlight bacterial rrn copy number as a key trait underpinning the life-history strategies and functional potential of microbial communities in response to long-term manure fertilization.

Keyword :

Life-history strategies Life-history strategies Manure Manure Metabolomics Metabolomics rRNA operon copy number rRNA operon copy number Soil function Soil function

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GB/T 7714 Bei, Shuikuan , Wu, Xingjie , Hou, Yarong et al. Bacterial rrn copy numbers linked to soil function and crop yield in long-term manure-fertilized soils [J]. | APPLIED SOIL ECOLOGY , 2025 , 209 .
MLA Bei, Shuikuan et al. "Bacterial rrn copy numbers linked to soil function and crop yield in long-term manure-fertilized soils" . | APPLIED SOIL ECOLOGY 209 (2025) .
APA Bei, Shuikuan , Wu, Xingjie , Hou, Yarong , Yuan, Huimin , Rensing, Christopher , Cui, Zhenling et al. Bacterial rrn copy numbers linked to soil function and crop yield in long-term manure-fertilized soils . | APPLIED SOIL ECOLOGY , 2025 , 209 .
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