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学者姓名:CHRISTOPHER GUENTHER THEODOR RENSING

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Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation SCIE
期刊论文 | 2026 , 288 | WATER RESEARCH
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Microbial biofilm-based hydrovoltaic electricity generators (BioHEGs) have recently been developed as promising and readily available platforms for green energy harvesting, despite their unsatisfactory output performances and unspecified mechanisms regarding electric current production. Herein, carbon quantum dots (CQDs) were used to construct a nano-biohybrid system with Shewanella oneidensis MR-1 (S. oneidensis), through which the CQDs/S. oneidensis BioHEG achieved a maximum open-circuit voltage of ca. 0.65 V and short-circuit current density of ca. 5.23 mu A & sdot;cm-2. In addition, both the hydrovoltaic effect and electrical conductivity of CQDs/S. oneidensis nano-biohybrids were noticeably improved due to enhanced secretion of extracellular polymeric substances (EPS) and accelerated electron transfer upon CQDs implantation, thereby leading to a nearly 14-fold increase in output power density compared to the bare S. oneidensis cells. Studies aimed to elucidate the underlying mechanism indicated that the hybridization of CQDs and S. oneidensis greatly promoted the metabolic synthesis of outer membrane c-type cytochromes (OM c-Cyts) and the extracellular secretion of riboflavin (RF), which was demonstrated to be decisive in the current producing process of the CQDs/ S. oneidensis BioHEG. This work thus proposes a viable strategy to boost the hydrovoltaic electricity generation capacity of microbial biofilms and provides a new perspective on the mechanism of accelerated electron transfer pathways inside BioHEGs.

Keyword :

Carbon quantum dots Carbon quantum dots Hydrovoltaic electricity generation Hydrovoltaic electricity generation Metabolic synthesis Metabolic synthesis Shewanella oneidensis Shewanella oneidensis

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GB/T 7714 Chen, Ting-Ting , Yan, Zhi-Wu , Cai, Feng-Ying et al. Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation [J]. | WATER RESEARCH , 2026 , 288 .
MLA Chen, Ting-Ting et al. "Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation" . | WATER RESEARCH 288 (2026) .
APA Chen, Ting-Ting , Yan, Zhi-Wu , Cai, Feng-Ying , He, Qiu-Xiang , You, Han-Hui , Rensing, Christopher et al. Carbon quantum dots boost microbial biofilm-based hydrovoltaic electricity generation . | WATER RESEARCH , 2026 , 288 .
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Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus SCIE
期刊论文 | 2025 , 513 (2) , 2807-2825 | PLANT AND SOIL
WoS CC Cited Count: 4
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Background and aimsThe long-term monoculture of tea plants leads to the accumulation of soil pathogens, disruption of the ecological balance of soil microbial communities. Intercropping systems, have been shown to enhance soil ecological stability. However, there is still a lack of research on the effects of the tea plant/Ophiopogon japonicus intercropping system on soil nutrient cycling and key microbial communities. MethodsA three-year field experiment was conducted to compare two treatments, monoculture and intercropping. Growth parameters such as tea plant height, canopy width, and bud number were investigated. High-throughput sequencing was used to evaluate the impact of the intercropping system on soil microbial community structure and function. ResultsThe intercropping system significantly promotes tea plant growth, with plant height, bud number, and fresh weight increasing by an average of 10.87%, 24.26%, and 23.53%, respectively. Soil organic matter content increased by an average of 23.83%. Simultaneously, the diversity and symbiotic relationships of soil microbial communities continue to strengthen, with significant enrichment observed in Proteobacteria, and Basidiomycota. Random forest modeling further indicated that intercropping enhanced the abundance of microbial taxa involved in organic matter decomposition, such as Pseudomonas, Sphingomonas, and Archaeorhizomyces, consistent with significant increases in cellulase and polyphenol oxidase activities (P < 0.05). Additionally, the intercropping system significantly reduced the relative abundance of pathogenic fungi such as Cladosporium and Curvularia. ConclusionIn tea cultivation practices, intercropping tea plants with O. japonicus can improve the soil micro-ecology of mountain tea plantations, restore soil vitality, and promote tea plant growth.

Keyword :

High-throughput sequencing High-throughput sequencing Intercropping Intercropping Soil microbiota Soil microbiota Tea plant/Ophiopogon japonicas Tea plant/Ophiopogon japonicas

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GB/T 7714 Shao, Shuaibo , Li, Zhongwei , Ma, Xiaoxiao et al. Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus [J]. | PLANT AND SOIL , 2025 , 513 (2) : 2807-2825 .
MLA Shao, Shuaibo et al. "Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus" . | PLANT AND SOIL 513 . 2 (2025) : 2807-2825 .
APA Shao, Shuaibo , Li, Zhongwei , Ma, Xiaoxiao , Cui, Jingru , Zhu, Yanqi , Li, Yuanping et al. Enhancing tea plant growth and soil microbial ecology through intercropping tea plants with Ophiopogon japonicus . | PLANT AND SOIL , 2025 , 513 (2) , 2807-2825 .
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Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau SCIE
期刊论文 | 2025 , 293 | MICROBIOLOGICAL RESEARCH
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Soil microbes, as intermediaries in plant-soil interactions, are closely linked to plant health in grassland ecosystems. In recent years, varying degrees of degradation have been observed in the alpine grasslands of the Qinghai-Tibet Plateau (QTP). Addressing grassland degradation, particularly under the influence of climate change, poses a global challenge. Understanding the factors driving grassland degradation on the QTP and developing appropriate mitigation measures is essential for the future sustainability of this fragile ecosystem. In this review, we discuss the environmental and anthropogenic factors affecting grassland degradation and the corresponding impacts on soil microbe community structure. We summarize the current research on the microbiome of the QTP, in particular the effect of vegetation, climate change, grazing, and land use, respectively on the alpine grassland microbiome. The results of these studies indicate that microbially mediated soil bioprocesses are important drivers of grassland ecosystem functional recovery. Therefore, a thorough understanding of the spatial distribution characteristics of the soil microbiome in alpine grasslands is required, and this necessitates an integrated approach in which the interactions among climatic factors, vegetation characteristics, and human activities are evaluated. Additionally, we assess and summarise current technological developments and prospects for applying soil microbiome technologies in sustainable agriculture, including: (i) single-strain inoculation, and (ii) inoculation of synthetic microbial communities, (iii) microbial community transplantation. Grassland restoration projects should be carried out with the understanding that each restoration measure has a unique effect on the soil microbial activity. We propose that the sustainable development of alpine grassland ecosystems can be achieved by adopting advanced microbiome technologies and integrating microbebased sustainable agricultural practices to maximise grassland biomass, increase soil carbon, and optimise soil nutrient cycling.

Keyword :

Alpine grassland Alpine grassland Ecosystem functions Ecosystem functions Influencing factors Influencing factors Microbiome techniques Microbiome techniques Soil microbes Soil microbes

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GB/T 7714 Zhang, Mingxu , Hu, Jinpeng , Zhang, Yuewei et al. Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 293 .
MLA Zhang, Mingxu et al. "Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau" . | MICROBIOLOGICAL RESEARCH 293 (2025) .
APA Zhang, Mingxu , Hu, Jinpeng , Zhang, Yuewei , Cao, Yanhua , Rensing, Christopher , Dong, Quanmin et al. Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau . | MICROBIOLOGICAL RESEARCH , 2025 , 293 .
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Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine SSCI
期刊论文 | 2025 , 14 (2) | LAND
WoS CC Cited Count: 1
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Soil metal(loid) pollution is a threat to ecological and environmental safety. The vegetation recovery in mining areas is of great significance for protecting soil resources. In this study, (1) we first gathered four types of soils to analyse their contamination degree, including tailings mud (TM), wasteland soil (TS) very near TM, as well as non-rhizosphere soils of pepper (PF) and maize (MF) in a farmland downstream from the TM (about 5 km). Geo-accumulation and potential ecological risk indices indicated that the soil samples were mainly polluted by antimony (Sb), arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb), and copper (Cu) to different degrees. Leachates of TM resulted in increased Sb, As, and Cd accumulation in TS. (2) Then, we sampled six local plants growing in the TS to assess the possibilities of using these plants as recovery vegetation in TS, of which Persicaria maackiana (Regel) Nakai ex T. Mori absorbed relatively high Sb concentrations in the leaves and roots. (3) After that, we collected rhizosphere soil and tissue samples from eight crops on the above farmland to assess their capacities as recovering vegetation of contaminated farmland soil, of which the fruits of maize accumulated the lowest concentrations of most monitored metal(loid)s (except for Pb). Further, we compared the differences in the bacterial community structure of MF, PF, TM, and TS to assess capacities of cultivating pepper and maize to improve soil microbial community structure. The MF displayed the best characteristics regarding the following attributes: (1) the highest concentrations of OMs and total P; (2) the highest OTU numbers and diversity of bacteria; and (3) the lowest abundance of bacteria with potentially pathogenic and stress-tolerant phenotypes.

Keyword :

mining area mining area multiple-metal(loid) contamination multiple-metal(loid) contamination soil ecological recovery soil ecological recovery soil microorganism soil microorganism

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GB/T 7714 Zhu, Yanming , Yang, Jigang , Zhang, Jiajia et al. Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine [J]. | LAND , 2025 , 14 (2) .
MLA Zhu, Yanming et al. "Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine" . | LAND 14 . 2 (2025) .
APA Zhu, Yanming , Yang, Jigang , Zhang, Jiajia , Tong, Yiran , Su, Hailan , Rensing, Christopher et al. Assessment of Ecological Recovery Potential of Various Plants in Soil Contaminated by Multiple Metal(loid)s at Various Sites near XiKuangShan Mine . | LAND , 2025 , 14 (2) .
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Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential SCIE
期刊论文 | 2025 , 118 (1) | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
WoS CC Cited Count: 2
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Two facultatively aerobic strains, designated SGZ-02(T) and SGZ-792(T), were isolated from plant Pennisetum sp., exhibiting the highest 16S rRNA gene sequence similarities with the type strains of Sphingomonas zeae LMG 28739(T) (98.6%) and Massilia forsythiae NBRC 114511(T) (98.4%), respectively. SGZ-02(T) grew between 5 and 45 degrees C, pH 5.0-11.0 and tolerated NaCl concentrations of 0-4% (w/v), whereas SGZ-792(T) thrived at 5-40 degrees C, pH 5.0-11.0 and NaCl tolerance to 0-3.5% (w/v). The major quinone of SGZ-02(T) was ubiquinone-10, with the dominant fatty acids being C-16:0 (13.5%), Summed Feature 3 (6.3%), C-14:02-OH (5.3%) and Summed Feature 8 (66.3%). SGZ-792(T) predominantly contained ubiquinone-8, with major fatty acids being C-16:0 (20.3%), Summed Feature 3 (5.0%) and Summed Feature 8 (54.7%). Average nucleotide identity and digital DNA-DNA hybridization values between two strains and their closest references strains were below the bacterial species threshold. Based on genotypic and phenotypic characteristics, strains SGZ-02(T) and SGZ-792(T) are proposed as novel species within the genera Sphingomonas and Massilia, respectively. The suggested names for the new species are Sphingomonas fuzhouensis sp. nov. (SGZ-02(T) = GDMCC 1.4033(T) = JCM 36769(T)) and Massilia phyllosphaerae sp. nov. (SGZ-792(T) = GDMCC 1.4211(T) = JCM 36643(T)), respectively.

Keyword :

Massilia phyllosphaerae sp. nov. Massilia phyllosphaerae sp. nov. Plant growth-promoting Plant growth-promoting Polyphasic taxonomy Polyphasic taxonomy Sphingomonas fuzhouensis sp. nov. Sphingomonas fuzhouensis sp. nov.

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GB/T 7714 Yao, Ling , Liu, Guo-Hong , Zhang, Shu-Yi et al. Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential [J]. | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY , 2025 , 118 (1) .
MLA Yao, Ling et al. "Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential" . | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 118 . 1 (2025) .
APA Yao, Ling , Liu, Guo-Hong , Zhang, Shu-Yi , Gao, Peng , Rensing, Christopher , Yang, Qiu-E et al. Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential . | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY , 2025 , 118 (1) .
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Isolation and pan-genome analysis of Acinetobacter junii SC22, a heavy metal(loid)s resistant and plant growth promoting bacterium, from the Zijin Gold and Copper mine SCIE
期刊论文 | 2025 , 38 (5) , 1615-1630 | BIOMETALS
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Excess heavy metals can be toxic to plants and microbes. The application of plant growth promoting bacteria (PGPB) in agriculture has gained global attention for promising sustainable development, including in heavy metals polluted soil. However, the metal resistance and plant growth promoting determinants of strains belonging to the genus Acinetobacter remain poorly understood. Here, we characterize strain SC22, and analyzed the complete genome of strain SC22. The influence of inoculating with this strain on soybean growth by pot experiment was also analyzed. Our results showed that strain SC22 displayed high As(III) and Cu(II) resistance and high IAA production. The presence of operons such as, merRACDT, zntA and 4 znuABC, czcABCD and arsRBCH, and trpRABCDFS encode functions enabling strain SC22 to survive under extremely highly heavy metal contaminated environments and also produce plant hormones. The inoculation of strain SC22 stimulated soybean growth. Strains belonging to the genus Acinetobacter have an open pan-genome, and ArsH encoded on the core genome displayed differences to ArsH encoded on the genome of other Acinetobacter that were isolated from different habitats. These findings suggest the potential use of strain Acinetobacter junii SC22 in bioremediation and subsequent plant growth promotion in heavy metal polluted environments.

Keyword :

Acinetobacter junii Acinetobacter junii Heavy metals Heavy metals PGPB PGPB Soybean Soybean

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GB/T 7714 Li, Yuanping , Yang, Xiaojun , Su, Junming et al. Isolation and pan-genome analysis of Acinetobacter junii SC22, a heavy metal(loid)s resistant and plant growth promoting bacterium, from the Zijin Gold and Copper mine [J]. | BIOMETALS , 2025 , 38 (5) : 1615-1630 .
MLA Li, Yuanping et al. "Isolation and pan-genome analysis of Acinetobacter junii SC22, a heavy metal(loid)s resistant and plant growth promoting bacterium, from the Zijin Gold and Copper mine" . | BIOMETALS 38 . 5 (2025) : 1615-1630 .
APA Li, Yuanping , Yang, Xiaojun , Su, Junming , Alwathnani, Hend , Ye, Jianghua , Rensing, Christopher . Isolation and pan-genome analysis of Acinetobacter junii SC22, a heavy metal(loid)s resistant and plant growth promoting bacterium, from the Zijin Gold and Copper mine . | BIOMETALS , 2025 , 38 (5) , 1615-1630 .
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Characterization and application of highly effective rhizobia isolated from Medicago ruthenica in alpine grassland ESCI
期刊论文 | 2025 , 4 (3) , 235-248 | GRASSLAND RESEARCH
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Background The symbiotic relationship between legume forages and their rhizobia is highly specific, and the effectiveness of rhizobial inoculants is often limited by local soil and climatic conditions. Therefore, identifying rhizobial strains that are well-adapted to specific environments is crucial for improving nitrogen fixation efficiency.Methods Four rhizobial strains were isolated from Medicago ruthenica (L.) Trautv and evaluated for their symbiotic performance with the same host plant. The most effective strain was identified based on key physiological parameters following inoculation. Response surface methodology was then applied to optimize the growth medium for the selected strain, GBXD30.Results Inoculation with strain GBXD30 increased plant biomass by 12%, enhanced the number of effective nodules by 3.5-fold, and boosted nitrogenase activity by 0.8-fold, compared to the reference strain USDA1844. Optimization of the fermentation medium via response surface analysis further demonstrated the potential of GBXD30 as a highly effective rhizobial inoculant suitable for alpine grassland conditions.Conclusions The targeted selection and application of effective rhizobial strains, such as GBXD30, are critical for maximizing nitrogen fixation in alpine legume forages. These findings offer valuable insights for developing rhizobial inoculants tailored to alpine ecosystems.

Keyword :

alpine grassland alpine grassland culture conditions culture conditions Medicago ruthenica Medicago ruthenica nitrogen fixation nitrogen fixation rhizobia rhizobia

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GB/T 7714 Zhang, Mingxu , Hu, Jinpeng , Boamah, Solomon et al. Characterization and application of highly effective rhizobia isolated from Medicago ruthenica in alpine grassland [J]. | GRASSLAND RESEARCH , 2025 , 4 (3) : 235-248 .
MLA Zhang, Mingxu et al. "Characterization and application of highly effective rhizobia isolated from Medicago ruthenica in alpine grassland" . | GRASSLAND RESEARCH 4 . 3 (2025) : 235-248 .
APA Zhang, Mingxu , Hu, Jinpeng , Boamah, Solomon , Lu, Zhaolong , Cao, Yanhua , Chu, Mengjiao et al. Characterization and application of highly effective rhizobia isolated from Medicago ruthenica in alpine grassland . | GRASSLAND RESEARCH , 2025 , 4 (3) , 235-248 .
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Fe(III)-dependent Nrf activity determines nitrate reduction partitioning in nitrate-reducing communities SCIE
期刊论文 | 2025 , 16 (11) | MBIO
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Identifying the factors that affect the nitrate reduction partitioning between dissimilatory nitrate reduction to ammonium (DNRA) and denitrification is crucial for mitigating nitrogen loss in ecosystems. Conventionally, the nutrient status of the environment (e.g., the carbon-to-nitrogen ratio) is recognized as the key determinant of nitrogen conversion pathways. Here, we report that the availability of Fe(III) regulates the nitrate reduction partitioning in Geobacter metallireducens and Alcaligenes faecalis co-culture. We controlled the availability of Fe(III) in the coculture medium and tracked nitrogen conversion dynamics and community composition. The results demonstrated that the coculture performed DNRA, contributed mainly by G. metallireducens under Fe(III)-replete conditions, while performing interspecies synergistic denitrification between both species under Fe(III)-depleted conditions. Nitrate/nitrite reductase activity calculations and mutation analyses indicated that nitrate reduction partitioning in the coculture was governed by the nitrite reductase (Nrf) activity of G. metallireducens, which was Fe(III)-dependent. Further validation in urban river water confirmed that Fe(III) supplementation significantly enhances DNRA activity. Our findings establish Fe(III) as a previously unrecognized regulator of microbial nitrogen retention, showing insights into strategies for managing nitrogen fluxes in agricultural and aquatic systems.IMPORTANCENitrogen is essential for life, but its loss from ecosystems through microbial processes like denitrification harms agricultural productivity and contributes to greenhouse gas emissions. Retaining nitrogen as ammonium via microbial dissimilatory nitrate reduction to ammonium (DNRA) could mitigate these issues, but the factors governing microbial prioritization of DNRA over denitrification remain unclear. Our study reveals that Fe(III) plays a critical, previously unrecognized role in steering this process. We show that Fe(III) availability determines whether the nitrate-reducing community conserves nitrogen as ammonium or releases it as gas, with implications for managing nitrogen in soils and waterways. By demonstrating Fe(III)'s ability to enhance nitrogen retention in environmental systems like urban rivers, our findings offer a new lever for sustainable agriculture and pollution control. This work bridges microbial ecology and environmental management, highlighting how trace metals shape nutrient cycles in ways that can be harnessed to protect ecosystem health.

Keyword :

denitrification denitrification dissimilatory nitrate reduction to ammonium dissimilatory nitrate reduction to ammonium Fe(III) cofactor Fe(III) cofactor interspecies synergistic denitrification interspecies synergistic denitrification nitrite reductase nitrite reductase nitrogen conversion nitrogen conversion

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GB/T 7714 Zhan, Ji , Zhang, Lu , Lai, Shuyao et al. Fe(III)-dependent Nrf activity determines nitrate reduction partitioning in nitrate-reducing communities [J]. | MBIO , 2025 , 16 (11) .
MLA Zhan, Ji et al. "Fe(III)-dependent Nrf activity determines nitrate reduction partitioning in nitrate-reducing communities" . | MBIO 16 . 11 (2025) .
APA Zhan, Ji , Zhang, Lu , Lai, Shuyao , Guo, Junhui , Lin, Tianqi , Liu, Guohong et al. Fe(III)-dependent Nrf activity determines nitrate reduction partitioning in nitrate-reducing communities . | MBIO , 2025 , 16 (11) .
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Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms
期刊论文 | 2025 , 47 (4) , 194-203 | 工程(英文)
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Phototrophy and chemotrophy are two dominant types of microbial metabolism.However,to date,the potential of the ubiquitous and versatile mechanical energy as a renewable energy source to drive the growth of microorganisms has remained unknown and not utilized.Here,we present evidence in favor of a previously unidentified metabolic pathway,in which the electronic energy produced from mechanical energy by the piezoelectric materials is used to support the growth of microorganisms.When electroactive microorganism Rhodopseudomonas palustris(R.palustris;with barium titanate nanoparticles)was mechanically stirred,a powerful biohybrid piezoelectric effect(BPE)enabled sustain-able carbon fixation coupled with nitrate reduction.Transcriptomic analyses demonstrated that mechanical stirring of the bacteria-barium titanate biohybrid led to upregulation of genes encoding functions involved in electron and energy transfer in R.palustris.Studies with other electroactive microorganisms suggested that the ability of microbes to utilize BPE may be a common phenomenon in the microbial world.Taken together,these findings imply a long-neglected and potentially important microbial metabolic pathway,with potential importance to microbial survival in the energy-limited environments.

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GB/T 7714 Guoping Ren , Jie Ye , Lu Liu et al. Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms [J]. | 工程(英文) , 2025 , 47 (4) : 194-203 .
MLA Guoping Ren et al. "Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms" . | 工程(英文) 47 . 4 (2025) : 194-203 .
APA Guoping Ren , Jie Ye , Lu Liu , Andong Hu , Kenneth H.Nealson , Christopher Rensing et al. Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms . | 工程(英文) , 2025 , 47 (4) , 194-203 .
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Rice Root Iron Plaque as a Mediator to Stimulate Methanotrophic Nitrogen Fixation SCIE
期刊论文 | 2025 , 59 (38) , 20411-20420 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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Iron plaque (IP) on rice root surfaces has been extensively documented as a natural barrier that effectively reduces contaminant bioavailability and accumulation. However, its regulatory mechanisms in rhizospheric methane oxidation and biological nitrogen fixation (BNF) remain elusive. This study reveals a previously unrecognized function of IP: mediating methanotrophic nitrogen fixation through coupled aerobic methane oxidation and IP reduction (Fe-MOX). Using a hydroponic coculture system integrating methane-oxidizing bacteria and rice seedlings, we demonstrated that IP enhanced microbial methane oxidation by 46.8% and significantly stimulated BNF rate by 33.6%, with methane-derived carbon accounting for 89.1% of the BNF energy source. Notably, dissolved iron removal did not diminish the BNF enhancement, excluding mediation by soluble iron species. Intriguingly, ferrihydrite supplementation at equivalent iron concentrations failed to replicate the BNF stimulation observed with IP, suggesting the indispensability of root-associated iron redox cycling. Mechanistic analyses identified that Methylosinus/Methylocystis species mediated Fe(III) reduction, synergistically collaborating with specific rhizobial strains to execute Fe-MOX-dependent BNF. These findings uncover a previously overlooked yet pronounced contribution of IP to BNF, providing novel insights for developing dual-strategy approaches to mitigate methane emissions and reduce nitrogen fertilizer dependency in paddy ecosystems.

Keyword :

iron oxyhydroxide plaque iron oxyhydroxide plaque metagenomic sequencing metagenomic sequencing methane-oxidizing bacteria methane-oxidizing bacteria nitrogen fixation nitrogen fixation

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GB/T 7714 Yu, Linpeng , Jia, Rong , Liu, Shiqi et al. Rice Root Iron Plaque as a Mediator to Stimulate Methanotrophic Nitrogen Fixation [J]. | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2025 , 59 (38) : 20411-20420 .
MLA Yu, Linpeng et al. "Rice Root Iron Plaque as a Mediator to Stimulate Methanotrophic Nitrogen Fixation" . | ENVIRONMENTAL SCIENCE & TECHNOLOGY 59 . 38 (2025) : 20411-20420 .
APA Yu, Linpeng , Jia, Rong , Liu, Shiqi , Li, Shuan , Shen, Yanxi , Rensing, Christopher et al. Rice Root Iron Plaque as a Mediator to Stimulate Methanotrophic Nitrogen Fixation . | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2025 , 59 (38) , 20411-20420 .
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