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学者姓名：曾建雄
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Abstract ：
The waste activated sludge (WAS) exhibits typical viscoelasticity due to the presence of viscous and gelling organics in extracellular polymeric substances (EPS). However, the positive role of reducing viscosity in WAS fermentation by degrading viscous polysaccharides has been historically overlooked. This work demonstrates the occurrence of viscous hyaluronan-like polysaccharides in the WAS for the first time. Approximately 6.8 % of bacteria, such as Zoogloea (1.0 %), were identified as the potential producers. The viscosity of hyaluronan could be significantly reduced by 99 % within 1 hour by the oriented hyaluronan-degrading consortium (HDC), and a reduction of 20 % was also observed for WAS after 24 h. This resulted in a 18 % improvement in methane production and a 35 % improvement in the maximum production rate in WAS fermentation. The conversion of viscous hyaluronan was mainly through the hyaluronan lyase (EC 4.2.2.1) dependent pathway. An unfamiliar genus of Paludibacter (9.6 %) was identified as a key bacterium, responsible for excreting five extracellular enzymes of EC 4.2.2.1, EC 3.2.1.35, EC 3.2.1.31, EC 3.2.1.52, and EC 3.2.1.180. Consequently, this study has elucidated reducing viscosity as a substantial factor in WAS fermentation by the oriented HDC, thus providing a novel paradigm to enhance methane production.
Keyword ：
Hyaluronan lyase Hyaluronan lyase Methane production Methane production Viscosity reduction Viscosity reduction Viscous hyaluronan-like polysaccharides Viscous hyaluronan-like polysaccharides Waste activated sludge Waste activated sludge
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| GB/T 7714 | Hu, Yan-Lin , Dai, Kun , Wang, Qing-Ting et al. Viscosity is a nonnegligible factor in the waste activated sludge fermentation: Taking hyaluronan as an example [J]. | WATER RESEARCH , 2025 , 279 . |
| MLA | Hu, Yan-Lin et al. "Viscosity is a nonnegligible factor in the waste activated sludge fermentation: Taking hyaluronan as an example" . | WATER RESEARCH 279 (2025) . |
| APA | Hu, Yan-Lin , Dai, Kun , Wang, Qing-Ting , Zhou, Chen-Yuan , Huang, Xing-Chen , Yang, Xiao-Fei et al. Viscosity is a nonnegligible factor in the waste activated sludge fermentation: Taking hyaluronan as an example . | WATER RESEARCH , 2025 , 279 . |
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The hydrolysis of structural extracellular polymeric substances (St-EPS) is considered a major limiting step in the anaerobic fermentation of waste activated sludge (WAS). However, the degradation of heteropolysaccharides, characterized by complex monomers of uronic acids and neutral saccharides in St-EPS, has rarely been reported. In this study, microbial-produced xanthan-like heteropolysaccharides, characterized by a blue filamentary film, were identified. The xanthan-producing bacteria comprised similar to 7.2% of total genera present in WAS. An xanthandegrading consortium (XDC) was enriched in an anaerobic batch reactor. This consortium could degrade Xanthan for over 90% and disrupt the gel structure of xanthan while promoting methane production from WAS by 29%. The xanthan degradation network consisting of extracellular enzymes and bacteria was elucidated by combining high-throughput sequencing, metagenomic, and metaproteomic analyses. Five enzymes were identified as responsible for hydrolyzing xanthan to monomers, including xanthan lyase, (beta-D-glucosidase, (beta-D-glucanase, alpha-D- mannosidase, and unsaturated glucuronyl hydrolase. Seven genera, including Paenibacillus (0.2%) and Clostridium (3.1%), were identified as key bacteria excreting one to five of the aforementioned enzymes. This study thus provides insights into the complex conversions in anaerobic digestion of WAS and gives a foundation for future optimization of this process.
Keyword ：
Degradation network Degradation network Heteropolysaccharides Heteropolysaccharides Methane production Methane production Xanthan-degrading consortium Xanthan-degrading consortium Xanthan lyase Xanthan lyase
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| GB/T 7714 | Zhou, Chen-Yuan , Dai, Kun , Lin, Yi-Peng et al. Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation [J]. | WATER RESEARCH X , 2025 , 27 . |
| MLA | Zhou, Chen-Yuan et al. "Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation" . | WATER RESEARCH X 27 (2025) . |
| APA | Zhou, Chen-Yuan , Dai, Kun , Lin, Yi-Peng , Huang, Xing-Chen , Hu, Yan-Lin , Chen, Xuan-Xin et al. Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation . | WATER RESEARCH X , 2025 , 27 . |
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Incineration is a common method for municipal sludge disposal; however, its effectiveness is hindered by high moisture content, substantial ash production, and low calorific value. This study proposes a systematic approach that integrates sludge dewatering and incineration processes by incorporating coal gangue to assist with sludge dewatering and blending it with pomelo peel waste for densification, ultimately producing high-quality fuel pellets. The addition of coal gangue significantly improved sludge permeability, reducing moisture content to 57.17 %, capillary suction time by 30.4 % and increasing calorific value by approximately 56 %. However, coal gangue content exceeding 50 % hindered drainage. Pomelo peel, used as a binder and combustion enhancer, improved the mechanical properties and combustion performance of the pellets, achieving a maximum calorific value of 14,289 kJ kg(-1) and compressive strength of 113.25 N min(-1) . Excessive pomelo peel (over 30 %) reduced wear resistance and adversely affected elasticity and density. Under optimized conditions (51.28 % sludge, 25.64 % coal gangue, 23.07 % pomelo peel, 100 MPa pressure), the pellets exhibited a denser structure, smoother surface, and combustion duration 5.09 times longer than powdered fuel. Pearson correlation and clustering analyses indicated that enhancing ductility and abrasion resistance is crucial for improving combustion performance, emphasizing the binder's significant impact on molding pressure. This research provides valuable insights into optimizing fuel pellet production from sludge.
Keyword ：
Coal gangue Coal gangue Co-pelletization Co-pelletization Fuel pellets Fuel pellets Municipal sludge disposal Municipal sludge disposal Pomelo peel Pomelo peel Sludge dewatering and incineration Sludge dewatering and incineration
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| GB/T 7714 | Zhang, Hao , Zhang, Ya-Li , He, Zhi-Yi et al. Optimizing sludge-to-fuel conversion: The synergistic effects of coal gangue and pomelo peel on pellet formation and combustion performance [J]. | ENERGY , 2025 , 325 . |
| MLA | Zhang, Hao et al. "Optimizing sludge-to-fuel conversion: The synergistic effects of coal gangue and pomelo peel on pellet formation and combustion performance" . | ENERGY 325 (2025) . |
| APA | Zhang, Hao , Zhang, Ya-Li , He, Zhi-Yi , Liu, Wen-Hui , Sun, Ping , Zeng, Yuan-Ping et al. Optimizing sludge-to-fuel conversion: The synergistic effects of coal gangue and pomelo peel on pellet formation and combustion performance . | ENERGY , 2025 , 325 . |
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The exchange of water between soil and air is a ubiquitous natural phenomenon. Evaporation and condensation are the two main processes that extract or release energy from the environment. However, due to the low energy density and complex interface of soil, the associated energy has long been overlooked and under-exploited. Here we demonstrate that the energy can be converted into electricity in situ through the hydrovoltaic effect within the soil itself. The soil-based hydrovoltaic electricity generators exhibit excellent reproducibility and stability, demonstrating the potential for scalable integration. A series voltage of over 13.4 V was achieved, placing it in the top five of integrated devices reported to date. By designing an integrated module suitable for amplifying natural electricity from both soil evaporation and soil condensation process, a parallel current of up to 18.62 mu A (0.06 m2) was obtained. The availability of soil demonstrates the great potential of the soil-based electricity generators. This finding demonstrates a green and sustainable technology for electricity generation, and points to possible biogeochemical processes due to the hydrovoltaic effect of soil.
Keyword ：
Evaporation-induced electricity generation Evaporation-induced electricity generation Hydrovoltaic effect Hydrovoltaic effect Moisture-induced electricity generation Moisture-induced electricity generation Soil-air water exchange Soil-air water exchange Soil electricity generation Soil electricity generation
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| GB/T 7714 | Diao, Youming , Hu, Qichang , Liu, Yingying et al. Natural electricity production from soil-air water exchange: A wide and untapped energy [J]. | NANO ENERGY , 2025 , 135 . |
| MLA | Diao, Youming et al. "Natural electricity production from soil-air water exchange: A wide and untapped energy" . | NANO ENERGY 135 (2025) . |
| APA | Diao, Youming , Hu, Qichang , Liu, Yingying , Zeng, Raymond Jianxiong , Zhou, Shungui , Chen, Man . Natural electricity production from soil-air water exchange: A wide and untapped energy . | NANO ENERGY , 2025 , 135 . |
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This study evaluates the utility of contact angle (CA) as an indicator of sludge dewatering performance, addressing inconsistencies in previous findings and the lack of a clear standard. We systematically compared four pretreatment methods-agar coating, in-situ filtration, lyophilization, and hot air drying-to assess contact angle efficacy. Measurement reliability was determined using standard deviation and coefficient of variation (CV), revealing that hot air drying (74.85 degrees f 4.24 degrees, CV 5.67 %) and lyophilization (71.48 degrees f 4.74 degrees, CV 6.6 %) exhibited significantly lower variability than agar coating (42.54 degrees f 12.24 degrees, CV 28.7 %) and in-situ filtration (53.35 degrees f 9.66 degrees, CV 18.1 %). Correlation matrix and Variable Importance in Projection (VIP) analyses indicated that contact angle only partially correlates with dewatering performance indicators, including water content, capillary suction time (CST), and specific resistance to filtration (SRF). Importantly, the predictive power of CA measurements varies across different pretreatment methods, underscoring its limitations as a standalone metric. Over-reliance on CA measurements may lead to biased conclusions. This study offers key perspectives on refining CA measurement methods and highlights the importance of comprehensive evaluation of sludge dewatering performance, guiding the development of more efficient treatment technologies.
Keyword ：
Contact angle Contact angle Dewatering performance Dewatering performance Hot air drying Hot air drying Sludge dewatering Sludge dewatering Surface wettability Surface wettability
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| GB/T 7714 | Chen, Xuan-Xin , He, Zhi-Yi , Gao, Yun-Yan et al. Evaluating the contact angle as an indicator of sludge dewatering performance: Limitations and methodological insights [J]. | ENVIRONMENTAL RESEARCH , 2025 , 277 . |
| MLA | Chen, Xuan-Xin et al. "Evaluating the contact angle as an indicator of sludge dewatering performance: Limitations and methodological insights" . | ENVIRONMENTAL RESEARCH 277 (2025) . |
| APA | Chen, Xuan-Xin , He, Zhi-Yi , Gao, Yun-Yan , Yuan, Yi-Wen , Zeng, Raymond Jianxiong , Wang, Hou-Feng . Evaluating the contact angle as an indicator of sludge dewatering performance: Limitations and methodological insights . | ENVIRONMENTAL RESEARCH , 2025 , 277 . |
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The effective treatment of food waste digestate is critical for reducing environmental pollution and mitigating carbon emissions, with deep dewatering playing a pivotal role. Conventional dewatering agents such as polyaluminum chloride (PAC) and polyacrylamide (PAM), commonly employed in municipal sludge treatment, exhibit limited efficacy when applied to food waste digestate due to the latter's high salinity and advanced fermentation stages. This study introduces polyethylene oxide (PEO) as a novel conditioning agent and investigates its dewatering performance in comparison to PAC and PAM, elucidating the underlying mechanism. PEO conditioning markedly improves deep-dewatering, reducing digestate moisture content from 93.11 % to 56.71 % and lowering specific resistance to filtration (SRF) by 90.3 %. In contrast, PAM, PAC, and their combination achieve moisture reductions to 81.18 %, 84.49 %, and 87.07 %, respectively, with significantly lower SRF improvements. PEO promotes the release of bound water by weakening solid-liquid binding energy, facilitating the transition of bound water to free water and enhancing overall water mobility. Moreover, compressibility coefficient analyses and X-ray computed tomography (X-CT) reveal that PEO treatment significantly increases filter cake porosity, with an effective porosity rate of 56.65 %, resulting in superior drainage performance. The enhanced dewatering efficiency of PEO stems from its ability to improve water permeability within the filter cake during compression, distinguishing its mechanism from traditional flocculation (PAM) and coagulation (PAC) approaches. This work highlights the potential of PEO as a highly effective solution for food waste digestate treatment in solid waste management, with its salt-resistant properties further extending its applicability to high-salinity waste streams.
Keyword ：
Conditioning Agent Conditioning Agent Deep-dewatering Deep-dewatering Food waste digestate Food waste digestate Permeability Permeability Polyethylene Oxide (PEO) Polyethylene Oxide (PEO) Solid waste management Solid waste management
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| GB/T 7714 | Wang, Hou-Feng , Gao, Yun-Yan , Zeng, Yuan-Ping et al. Overcoming deep-dewatering challenges in food waste digestate with polyethylene oxide as an innovative conditioning agent [J]. | WATER RESEARCH , 2025 , 269 . |
| MLA | Wang, Hou-Feng et al. "Overcoming deep-dewatering challenges in food waste digestate with polyethylene oxide as an innovative conditioning agent" . | WATER RESEARCH 269 (2025) . |
| APA | Wang, Hou-Feng , Gao, Yun-Yan , Zeng, Yuan-Ping , Chen, Xuan-Xin , He, Zhi-Yi , Zeng, Raymond Jianxiong . Overcoming deep-dewatering challenges in food waste digestate with polyethylene oxide as an innovative conditioning agent . | WATER RESEARCH , 2025 , 269 . |
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Wastewater treatment significantly contributes to greenhouse gas emissions, which are further exacerbated by the environmental impact of external chemical additions. In response, microbial electrochemical wastewater refining has gained prominence at the interdisciplinary frontier of wastewater resource recovery and green bio-manufacturing. Significant progress has been made in utilizing active electrodes to stimulate CO2 fixation rates, applying "binary electron donors" to produce high-value-added chemicals, and developing novel processes and equipment. This review explores various aspects of microbial electrochemical wastewater refining, including microbial electrochemical monitoring of water quality, chemical synthesis from diverse carbon sources, and the deployment of pilot-scale systems for generating electricity, hydrogen, and methane, as well as for in-situ remediation. Additionally, it discusses the challenges and future directions, highlighting the importance of understanding mechanisms, advancing electrocatalyst and microbial engineering, and innovating hybrid processes. In conclusion, the widespread adoption of microbial electrochemical wastewater refining is emphasized for resource recovery and sustainable chemical production, ultimately reducing environmental impact. (c) 2024 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keyword ：
CO 2 reduction CO 2 reduction Extracellular electron transfer Extracellular electron transfer Microbial electrosynthesis Microbial electrosynthesis Resources recovery Resources recovery Wastewater treatment Wastewater treatment
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| GB/T 7714 | Chu, Na , Li, Daping , Zeng, Raymond Jianxiong et al. Microbial Electrochemical Wastewater Refining [J]. | ENGINEERING , 2025 , 46 : 245-256 . |
| MLA | Chu, Na et al. "Microbial Electrochemical Wastewater Refining" . | ENGINEERING 46 (2025) : 245-256 . |
| APA | Chu, Na , Li, Daping , Zeng, Raymond Jianxiong , Jiang, Yong , Liang, Peng . Microbial Electrochemical Wastewater Refining . | ENGINEERING , 2025 , 46 , 245-256 . |
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本发明公开了一种电化学生物耦合生产酿酒酵母蛋白的方法，其是先将二氧化碳通过电化学模块还原生成甲酸，再利用生成的甲酸作为碳源和底物，通过生物模块生产酿酒酵母蛋白；其中，所述电化学模块包括气体室、填充有固态电解质的提取室、阳极室及设置于气体室和提取室之间的阴极，设置于阳极室和提取室之间的阳极；所述生物模块由隔膜分隔为两室结构，一是用于使甲酸转化为乙酸的产乙酸菌室，另一个是利用乙酸生产为酵母蛋白的酵母室。本发明通过电催化二氧化碳还原产甲酸，再利用甲酸为底物产乙酸，并进一步利用乙酸合成菌体的级联反应，实现酿酒酵母蛋白的生产，有望应用于绿色生物制造。
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| GB/T 7714 | 蒋永 , 吴晓冰 , 褚娜 et al. 一种电化学生物耦合生产酿酒酵母蛋白的方法 : CN202510114087.3[P]. | 2025-01-24 . |
| MLA | 蒋永 et al. "一种电化学生物耦合生产酿酒酵母蛋白的方法" : CN202510114087.3. | 2025-01-24 . |
| APA | 蒋永 , 吴晓冰 , 褚娜 , 曾建雄 . 一种电化学生物耦合生产酿酒酵母蛋白的方法 : CN202510114087.3. | 2025-01-24 . |
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本发明公开了一种二氧化碳还原制甲酸用固态电解质反应器性能的快速表征方法，其是利用系列甲酸溶液中的甲酸浓度与电导率建立数学关系，并用于快速拟合固态电解质反应器中提取液出水的甲酸浓度，再计算法拉第效率，从而对固态电解质反应器的性能进行判定。该方法可实现二氧化碳还原制甲酸用固态电解质反应器性能的快速评定，并同样适用于产乙酸、产过氧化氢、产氨等的固态电解质反应器性能快速评定。
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| GB/T 7714 | 蒋永 , 褚娜 , 吴晓冰 et al. 一种固态电解质反应器性能的快速评定方法 : CN202510114067.6[P]. | 2025-01-24 . |
| MLA | 蒋永 et al. "一种固态电解质反应器性能的快速评定方法" : CN202510114067.6. | 2025-01-24 . |
| APA | 蒋永 , 褚娜 , 吴晓冰 , 曾建雄 . 一种固态电解质反应器性能的快速评定方法 : CN202510114067.6. | 2025-01-24 . |
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本发明公开了一种厌氧降解生物质生产甲烷和有机酸的微生物菌群及其培养方法与应用，其中微生物菌群包括：5％‑30％Clostridium属、2％‑10％Paludibacter属、1％‑25％Thermovirga属、2％‑20％DMER64属、2％‑20％Mesotoga属、1％‑10％Methanosaeta以及1％‑10％Methanobacterium；该菌群的培养方法，包括：S1：取果胶、秸秆、落叶，烘干后用研钵将秸秆研磨至粉末状；S2：将果胶降解菌和剩余污泥混合接种于培养瓶中，加入培养基，并在培养基中加入步骤S1得到的粉末；S3：通入氮气和二氧化碳的混合气体，密封培养瓶，在温度为30～38℃的条件下连续培养30～100天，进行果胶、纤维素水解菌群富集；该微生物菌群能够快速水解转化秸秆和落叶等生物质为甲烷、乙酸、丙酸、丁酸或己酸产品；相对于碱处理、高温处理等常规预处理方式具有产量高、运行条件温和运行成本低等优势。
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| GB/T 7714 | 张放 , 曹玉尔 , 戴昆 et al. 一种厌氧降解生物质生产甲烷和有机酸的微生物菌群及其培养方法与应用 : CN202510115408.1[P]. | 2025-01-24 . |
| MLA | 张放 et al. "一种厌氧降解生物质生产甲烷和有机酸的微生物菌群及其培养方法与应用" : CN202510115408.1. | 2025-01-24 . |
| APA | 张放 , 曹玉尔 , 戴昆 , 宋梁 , 曾建雄 . 一种厌氧降解生物质生产甲烷和有机酸的微生物菌群及其培养方法与应用 : CN202510115408.1. | 2025-01-24 . |
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