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学者姓名：曾建雄
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Abstract ：
The waste activated sludge (WAS) exhibits typical viscoelasticity due to the presence of viscous and gelling organics in extracellular polymeric substances (EPS). However, the positive role of reducing viscosity in WAS fermentation by degrading viscous polysaccharides has been historically overlooked. This work demonstrates the occurrence of viscous hyaluronan-like polysaccharides in the WAS for the first time. Approximately 6.8 % of bacteria, such as Zoogloea (1.0 %), were identified as the potential producers. The viscosity of hyaluronan could be significantly reduced by 99 % within 1 hour by the oriented hyaluronan-degrading consortium (HDC), and a reduction of 20 % was also observed for WAS after 24 h. This resulted in a 18 % improvement in methane production and a 35 % improvement in the maximum production rate in WAS fermentation. The conversion of viscous hyaluronan was mainly through the hyaluronan lyase (EC 4.2.2.1) dependent pathway. An unfamiliar genus of Paludibacter (9.6 %) was identified as a key bacterium, responsible for excreting five extracellular enzymes of EC 4.2.2.1, EC 3.2.1.35, EC 3.2.1.31, EC 3.2.1.52, and EC 3.2.1.180. Consequently, this study has elucidated reducing viscosity as a substantial factor in WAS fermentation by the oriented HDC, thus providing a novel paradigm to enhance methane production.
Keyword ：
Hyaluronan lyase Hyaluronan lyase Methane production Methane production Viscosity reduction Viscosity reduction Viscous hyaluronan-like polysaccharides Viscous hyaluronan-like polysaccharides Waste activated sludge Waste activated sludge
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| GB/T 7714 | Hu, Yan-Lin , Dai, Kun , Wang, Qing-Ting et al. Viscosity is a nonnegligible factor in the waste activated sludge fermentation: Taking hyaluronan as an example [J]. | WATER RESEARCH , 2025 , 279 . |
| MLA | Hu, Yan-Lin et al. "Viscosity is a nonnegligible factor in the waste activated sludge fermentation: Taking hyaluronan as an example" . | WATER RESEARCH 279 (2025) . |
| APA | Hu, Yan-Lin , Dai, Kun , Wang, Qing-Ting , Zhou, Chen-Yuan , Huang, Xing-Chen , Yang, Xiao-Fei et al. Viscosity is a nonnegligible factor in the waste activated sludge fermentation: Taking hyaluronan as an example . | WATER RESEARCH , 2025 , 279 . |
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The hydrolysis of structural extracellular polymeric substances (St-EPS) is considered a major limiting step in the anaerobic fermentation of waste activated sludge (WAS). However, the degradation of heteropolysaccharides, characterized by complex monomers of uronic acids and neutral saccharides in St-EPS, has rarely been reported. In this study, microbial-produced xanthan-like heteropolysaccharides, characterized by a blue filamentary film, were identified. The xanthan-producing bacteria comprised similar to 7.2% of total genera present in WAS. An xanthandegrading consortium (XDC) was enriched in an anaerobic batch reactor. This consortium could degrade Xanthan for over 90% and disrupt the gel structure of xanthan while promoting methane production from WAS by 29%. The xanthan degradation network consisting of extracellular enzymes and bacteria was elucidated by combining high-throughput sequencing, metagenomic, and metaproteomic analyses. Five enzymes were identified as responsible for hydrolyzing xanthan to monomers, including xanthan lyase, (beta-D-glucosidase, (beta-D-glucanase, alpha-D- mannosidase, and unsaturated glucuronyl hydrolase. Seven genera, including Paenibacillus (0.2%) and Clostridium (3.1%), were identified as key bacteria excreting one to five of the aforementioned enzymes. This study thus provides insights into the complex conversions in anaerobic digestion of WAS and gives a foundation for future optimization of this process.
Keyword ：
Degradation network Degradation network Heteropolysaccharides Heteropolysaccharides Methane production Methane production Xanthan-degrading consortium Xanthan-degrading consortium Xanthan lyase Xanthan lyase
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| GB/T 7714 | Zhou, Chen-Yuan , Dai, Kun , Lin, Yi-Peng et al. Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation [J]. | WATER RESEARCH X , 2025 , 27 . |
| MLA | Zhou, Chen-Yuan et al. "Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation" . | WATER RESEARCH X 27 (2025) . |
| APA | Zhou, Chen-Yuan , Dai, Kun , Lin, Yi-Peng , Huang, Xing-Chen , Hu, Yan-Lin , Chen, Xuan-Xin et al. Elucidating the complex hydrolysis and conversion network of xanthan-like extracellular heteropolysaccharides in waste activated sludge fermentation . | WATER RESEARCH X , 2025 , 27 . |
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The increasing complexity of experimental designs and the volume of data in the microbiome field, along with the diversification of omics data types, pose substantial challenges to statistical analysis and visualization. Here we present a step-by-step protocol based on the R microeco package (https://github.com/ChiLiubio/microeco) that details the statistical analysis and visualization of microbiome data. The omics data types shown consist of amplicon sequencing data, metagenomic sequencing data and nontargeted metabolomics data. The analysis of amplicon sequencing data specifically involves data preprocessing and normalization, core taxa, alpha diversity, beta diversity, differential abundance testing and machine learning. We consider various data analysis scenarios in each section to exhibit the comprehensiveness of the protocol. We emphasize that different normalized data produced by various methods are selected for subsequent analysis of each part based on the best analytical practices. Additionally, in the differential abundance test analysis, we adopt parametric community simulation to enable the performance evaluation of various testing approaches. For the analysis of metagenomic data, the focus is on how bioinformatic analysis data are read and preprocessed, which refers to the major usage differences from amplicon sequencing data. For metabolomics data, we mainly demonstrate the differential test, machine learning and association analysis with microbial abundances. To address some complex analyses, this protocol extensively combines different types of methods to build an analysis pipeline. This protocol is more comprehensive and scalable compared with alternative methods. The provided R codes can run in about 6 h on a laptop computer.
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| GB/T 7714 | Liu, Chi , Mansoldo, Felipe R. P. , Li, Hankang et al. A workflow for statistical analysis and visualization of microbiome omics data using the R microeco package [J]. | NATURE PROTOCOLS , 2025 . |
| MLA | Liu, Chi et al. "A workflow for statistical analysis and visualization of microbiome omics data using the R microeco package" . | NATURE PROTOCOLS (2025) . |
| APA | Liu, Chi , Mansoldo, Felipe R. P. , Li, Hankang , Vermelho, Alane Beatriz , Zeng, Raymond Jianxiong , Li, Xiangzhen et al. A workflow for statistical analysis and visualization of microbiome omics data using the R microeco package . | NATURE PROTOCOLS , 2025 . |
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本发明公开了一种电化学生物耦合生产酿酒酵母蛋白的方法，其是先将二氧化碳通过电化学模块还原生成甲酸，再利用生成的甲酸作为碳源和底物，通过生物模块生产酿酒酵母蛋白；其中，所述电化学模块包括气体室、填充有固态电解质的提取室、阳极室及设置于气体室和提取室之间的阴极，设置于阳极室和提取室之间的阳极；所述生物模块由隔膜分隔为两室结构，一是用于使甲酸转化为乙酸的产乙酸菌室，另一个是利用乙酸生产为酵母蛋白的酵母室。本发明通过电催化二氧化碳还原产甲酸，再利用甲酸为底物产乙酸，并进一步利用乙酸合成菌体的级联反应，实现酿酒酵母蛋白的生产，有望应用于绿色生物制造。
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| GB/T 7714 | 蒋永 , 吴晓冰 , 褚娜 et al. 一种电化学生物耦合生产酿酒酵母蛋白的方法 : CN202510114087.3[P]. | 2025-01-24 . |
| MLA | 蒋永 et al. "一种电化学生物耦合生产酿酒酵母蛋白的方法" : CN202510114087.3. | 2025-01-24 . |
| APA | 蒋永 , 吴晓冰 , 褚娜 , 曾建雄 . 一种电化学生物耦合生产酿酒酵母蛋白的方法 : CN202510114087.3. | 2025-01-24 . |
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本发明公开了一种二氧化碳还原制甲酸用固态电解质反应器性能的快速表征方法，其是利用系列甲酸溶液中的甲酸浓度与电导率建立数学关系，并用于快速拟合固态电解质反应器中提取液出水的甲酸浓度，再计算法拉第效率，从而对固态电解质反应器的性能进行判定。该方法可实现二氧化碳还原制甲酸用固态电解质反应器性能的快速评定，并同样适用于产乙酸、产过氧化氢、产氨等的固态电解质反应器性能快速评定。
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| GB/T 7714 | 蒋永 , 褚娜 , 吴晓冰 et al. 一种固态电解质反应器性能的快速评定方法 : CN202510114067.6[P]. | 2025-01-24 . |
| MLA | 蒋永 et al. "一种固态电解质反应器性能的快速评定方法" : CN202510114067.6. | 2025-01-24 . |
| APA | 蒋永 , 褚娜 , 吴晓冰 , 曾建雄 . 一种固态电解质反应器性能的快速评定方法 : CN202510114067.6. | 2025-01-24 . |
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Solid-state electrolyte (SSE) reactors represent a promising platform for carbon capture and utilization (CCU), particularly due to their ability to directly convert CO2 to high-purity liquid products. However, conventional nuclear magnetic resonance (NMR) and chromatography techniques for performance characterization are often time-consuming, instrument-intensive, and procedurally complex. This study presents a rapid characterization method that determines Faradaic efficiency (FE) by measuring the conductivity of the extraction solution from the SSE reactors and applying an empirical correlation to estimate formic acid concentration. The method exhibits strong robustness across a wide range of reactor configurations and components, including variations in separator materials, gas diffusion electrode (GDE) properties, anode types, SSE compositions, and electrocatalysts. Comparative analysis with high-performance liquid chromatography (HPLC) confirms the reliability of the conductivity-based method, yielding an average relative error of 6.1% in concentration and FE. By enabling rapid and accurate performance assessment, this method supports the efficient screening of SSE reactor designs and operating conditions, thereby accelerating the development of sustainable CO2 valorization technologies.
Keyword ：
CO2 electrolysis CO2 electrolysis conductivity conductivity Faraday efficiency Faraday efficiency formic acid formic acid gas diffusion electrode gas diffusion electrode
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| GB/T 7714 | Chu, Na , Zeng, Raymond Jianxiong , Jiang, Yong et al. Conductivity-Based Rapid Characterization of Porous Solid-State Electrolyte Reactors [J]. | ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS , 2025 , 12 (8) : 963-969 . |
| MLA | Chu, Na et al. "Conductivity-Based Rapid Characterization of Porous Solid-State Electrolyte Reactors" . | ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS 12 . 8 (2025) : 963-969 . |
| APA | Chu, Na , Zeng, Raymond Jianxiong , Jiang, Yong , Liang, Peng . Conductivity-Based Rapid Characterization of Porous Solid-State Electrolyte Reactors . | ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS , 2025 , 12 (8) , 963-969 . |
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本发明涉及一种生物耦合电池及其制备方法和应用。生物耦合电池包括电化学二氧化碳还原模块和微生物电化学模块；使用电化学二氧化碳还原模块进行快速充电，使用微生物电化学模块进行持久放电，因此具有快速充电、持久放电的特征；所述电化学二氧化碳还原模块中提取室中使用提取剂以提取二氧化碳还原的产物；所述二氧化碳还原的产物可被所述微生物电化学模块中的生物阳极代谢以产生生物电流；生物电流可作为能源回收的方式，也可以进一步作为水质预警的电信号。基于本发明，可发展新型多功能的电化学微生物耦合技术平台。
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| GB/T 7714 | 蒋永 , 褚娜 , 曾建雄 . 一种生物耦合电池及其制备方法和应用 : CN202311340850.1[P]. | 2023-10-17 . |
| MLA | 蒋永 et al. "一种生物耦合电池及其制备方法和应用" : CN202311340850.1. | 2023-10-17 . |
| APA | 蒋永 , 褚娜 , 曾建雄 . 一种生物耦合电池及其制备方法和应用 : CN202311340850.1. | 2023-10-17 . |
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The distribution and transformation of water content critically influence sludge dewatering performance and remain central to sludge treatment research. Low-field nuclear magnetic resonance (LF-NMR), known for its nondestructive and rapid analysis, has emerged as a vital tool for characterizing water distribution in sludge, though its limitations require further investigation. This study employed LF-NMR to analyze T2relaxation spectra of sludge under various pretreatments and with differing water contents (97 %-62 %). Results indicated that, despite similar overall water content, sludge subjected to different pretreatments exhibited significant variations in T2peak counts and times. Notably, T2,, T22, T2s, and T2a showed significant exponential relationships with water content (p < 0.0001). The transformation and migration of adsorbed, interstitial, and free water demonstrated complex bidirectional continuity. Removal of adsorbed and interstitial water significantly altered sludge structure, diminishing the reversibility of water reabsorption, whereas removal of free water had minimal impact on microstructure. Overlapping T2relaxation peaks suggested continuous transitions among water states, challenging the traditional discrete categorization. Neglecting changes in T2 peak times may lead to misinterpretation of water states and affect assessments of dewatering performance. While LF-NMR shows great potential, its limitations in resolving overlapping peaks and quantitatively assessing water states must be acknowledged. Consequently, LF-NMR should be regarded as a qualitative or semi-quantitative method rather than a purely quantitative tool as often assumed. Future research should integrate higher-resolution T,-T2two-dimensional NMR techniques with advanced data processing to more accurately elucidate the mechanisms of water migration and transformation within sludge.
Keyword ：
Low-field NMR (LF-NMR) Low-field NMR (LF-NMR) Sludge dewatering Sludge dewatering T 2 relaxation time T 2 relaxation time Water distribution Water distribution Water migration and transformation Water migration and transformation Water state characterization Water state characterization
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| GB/T 7714 | Sun, Ping , Chen, Xuan-Xin , Zeng, Yuan-Ping et al. Reassessing LF-NMR applications: Unraveling water state transformations in sludge and their implications for dewatering performance [J]. | WATER RESEARCH , 2025 , 284 . |
| MLA | Sun, Ping et al. "Reassessing LF-NMR applications: Unraveling water state transformations in sludge and their implications for dewatering performance" . | WATER RESEARCH 284 (2025) . |
| APA | Sun, Ping , Chen, Xuan-Xin , Zeng, Yuan-Ping , He, Zhi-Yi , Gao, Yun-Yan , Zeng, Raymond Jianxiong et al. Reassessing LF-NMR applications: Unraveling water state transformations in sludge and their implications for dewatering performance . | WATER RESEARCH , 2025 , 284 . |
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本发明涉及固废资源化利用技术领域，具体公开了一种基于气化细渣的固体替代燃料，按重量份计，包括气化细渣10~40份、城市污泥10~40份、柑橘类水果的副产品30~70份，所述固体替代燃料的成型密度为1.28g/cm3、抗压强度为1.22N/cm2以及比能耗为10.65J/g。具体制备过程包括以下步骤：1）对原料分别进行预处理；2）将预处理好的原料进行混合；3）将混合后的原料加入造粒设备挤压成表面光滑的圆柱形固体替代燃料。本发明以气化细渣为基础，添加城市污泥与生物质制得固体替代燃料，改善气化细渣燃烧热值不高的缺陷，并且能够降低灰分含量，使其满足资源化利用的条件，具有较高密度与抗压强度，便于该固体替代燃料作为燃料产品运输和保存，为气化细渣的利用提供新的技术思路和方向。
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| GB/T 7714 | 王厚锋 , 何志毅 , 曾远苹 et al. 一种基于气化细渣的固体替代燃料及其制备方法 : CN202411627246.1[P]. | 2024-11-14 . |
| MLA | 王厚锋 et al. "一种基于气化细渣的固体替代燃料及其制备方法" : CN202411627246.1. | 2024-11-14 . |
| APA | 王厚锋 , 何志毅 , 曾远苹 , 曾建雄 , 王涛 , 钟晓强 et al. 一种基于气化细渣的固体替代燃料及其制备方法 : CN202411627246.1. | 2024-11-14 . |
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The solid-liquid separation of food waste anaerobic digestate residue (FD) is a crucial step in maximizing the efficiency and sustainability of anaerobic digestion processes. However, the high salinity and organic content of FD significantly hinder conventional dewatering methods, making deep-dewatering particularly challenging. This study introduces a composite conditioning strategy using basic aluminum chloride (BAC) and a complex quaternary ammonium salt surfactant (G agent) to enhance the digestate's drainage performance and dewatering efficiency by constructing a skeletal structure within it. Experimental results showed that BAC+G composite conditioning significantly reduced the water content of the digestate from 90.69 +/- 0.36 to 54.19 +/- 0.16%, achieving deep dewatering that was unattainable with BAC or G agent alone. On a macroscopic scale, the BAC+G treatment enhanced floc strength and increased flocculated particle size to 469.07 +/- 0.73 mu m, approximately 18 times larger than untreated digestate, which significantly mitigated clogging and improved the permeability coefficient from 2.40 x 10-6 to 9.79 x 10-6 cm/s, ensuring smooth water discharge. Microscopically, the treatment increased effective porosity by 34.90%, reduced tortuosity to 1.45, and improved overall permeability (4.41), accelerating water discharge and further enhancing the dewatering performance. Additionally, BAC+G composite conditioning transformed floc particles to hydrophobic, lowered the interfacial free energy, and formed stable structures, further enhancing dewatering performance. These findings demonstrate that combining flocculation with skeletal structure formation is critical for achieving deep-dewatering of a high-salinity food waste digestate. This research provides a promising approach for improving digestate management and could have broader implications for the sustainable treatment of high-moisture organic waste streams.
Keyword ：
composite conditioning composite conditioning deep-dewatering deep-dewatering food waste digestate food waste digestate high-salinitydigestate high-salinitydigestate skeletalstructure skeletalstructure
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| GB/T 7714 | Gao, Yun-Yan , Zeng, Yuan-Ping , Chen, Xuan-Xin et al. Skeletal Structure-Based Conditioning for Improved Deep-Dewatering Efficiency of High-Salinity Food Waste Digestate [J]. | ACS ES&T ENGINEERING , 2025 , 5 (3) : 620-630 . |
| MLA | Gao, Yun-Yan et al. "Skeletal Structure-Based Conditioning for Improved Deep-Dewatering Efficiency of High-Salinity Food Waste Digestate" . | ACS ES&T ENGINEERING 5 . 3 (2025) : 620-630 . |
| APA | Gao, Yun-Yan , Zeng, Yuan-Ping , Chen, Xuan-Xin , He, Zhi-Yi , Zeng, Raymond Jianxiong , Wang, Hou-Feng . Skeletal Structure-Based Conditioning for Improved Deep-Dewatering Efficiency of High-Salinity Food Waste Digestate . | ACS ES&T ENGINEERING , 2025 , 5 (3) , 620-630 . |
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