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学者姓名：崔海涛
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Abstract ：
Mitogen-activated protein kinases (MAPKs/MPKs) are pivotal regulators in many stress-signaling pathways in plants. The dual phosphorylation of the TXY motif by MAP kinase kinases (MKKs) is essential for activating MAPKs. Here, we reveal a mechanism for MAPK activation that bypasses the need for MKKs. We identified rice (Oryza sativa) calcium-dependent protein kinase 5 (OsCPK5) and OsCPK13 as positive regulators in salt stress tolerance. These kinases are essential for the full activation of OsMPK3 and OsMPK6 in response to elevated sodium levels, with both OsMPK3 and OsMPK6 also acting as positive regulators in rice salt tolerance. Biochemical analysis demonstrated that OsCPK5/13 directly interact with and activate OsMPK3/6 by phosphorylating the TXY motif in vitro and in vivo. Additionally, we have discovered that OsCPK5/13 relocate from the cell membrane to the nucleus in response to salt stress. This process relies on their N-terminal myristoylation and a calcium-dependent phosphorylation event within the N-terminus. Our results elucidate a MAPK activation pathway in rice that is independent of traditional MKK-mediated phosphorylation, highlighting the crucial roles of OsCPK5 and OsCPK13 in directly phosphorylating and activating OsMPK3/6, which are important for rice tolerance to salt stress. Two calcium-dependent protein kinases directly phosphorylate and activate 2 mitogen-activated protein kinases that play a crucial role in rice salt stress tolerance.
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| GB/T 7714 | Su, Shiqi , Jiang, Yimin , Zhu, Xiang et al. Calcium-dependent protein kinases 5 and 13 enhance salt tolerance in rice by directly activating OsMPK3/6 kinases [J]. | PLANT PHYSIOLOGY , 2024 , 196 (4) : 3033-3047 . |
| MLA | Su, Shiqi et al. "Calcium-dependent protein kinases 5 and 13 enhance salt tolerance in rice by directly activating OsMPK3/6 kinases" . | PLANT PHYSIOLOGY 196 . 4 (2024) : 3033-3047 . |
| APA | Su, Shiqi , Jiang, Yimin , Zhu, Xiang , Yu, Shibo , Wang, Fuxiang , Xue, Li et al. Calcium-dependent protein kinases 5 and 13 enhance salt tolerance in rice by directly activating OsMPK3/6 kinases . | PLANT PHYSIOLOGY , 2024 , 196 (4) , 3033-3047 . |
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Transcription factors (TFs) play essential roles in transcriptional reprogramming during activation of plant immune responses to pathogens. OsSPL10 (SQUAMOSA promoter binding protein-like10) is an important TF regulating trichome development and salt tolerance in rice. Here we report that knockout of OsSPL10 reduces whereas its overexpression enhances rice resistance to blast disease. OsSPL10 positively regulates chitin-induced immune responses including reactive oxygen species (ROS) burst and callose deposition. We show that OsSPL10 physically associates with OsJAmyb, an important TF involved in jasmonic acid (JA) signaling, and positively regulates its protein stability. We then prove that OsJAmyb positively regulates resistance to blast. Our results reveal a molecular module consisting of OsSPL10 and OsJAmyb that positively regulates blast resistance. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC
Keyword ：
Immunity Immunity Jasmonate Jasmonate Oryza sativa Oryza sativa OsSPL10 OsSPL10 Transcription factor Transcription factor
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| GB/T 7714 | Zhong, Zaofa , Zhong, Lijing , Zhu, Xiang et al. Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice [J]. | CROP JOURNAL , 2024 , 12 (1) : 301-307 . |
| MLA | Zhong, Zaofa et al. "Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice" . | CROP JOURNAL 12 . 1 (2024) : 301-307 . |
| APA | Zhong, Zaofa , Zhong, Lijing , Zhu, Xiang , Jiang, Yimin , Zheng, Yihong , Lan, Tao et al. Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice . | CROP JOURNAL , 2024 , 12 (1) , 301-307 . |
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【目的】深入挖掘香蕉枯萎病菌致病相关基因，并解析其基因特性，为香蕉抗枯萎病品种选育及新药物靶标的开发打下基础。【方法】基于遗传背景将供试299株香蕉枯萎病菌的致病力分别依据数量性状和质量性状进行分组，使用混合线性模型的Gemma软件，运用全基因组关联分析（GWAS）技术对香蕉枯萎病菌致病力表型与全基因组重测序数据进行关联分析；进一步采用ProtParam、SingleIP和SAMRT等生物信息学软件解析候选基因的理化性质及初级结构。【结果】运用GWAS共关联到151个与致病力相关的单核苷酸多态性（SNP）位点。初步确定3个不同类型基因FocScp、FocChp和FocGhf12与枯萎病菌致病力密切相关，进一步对FocScp、FocChp和FocGhf12基因的编码蛋白进行生物信息学分析并预测其功能，结果显示，FocScp基因的cDNA编码区全长948 bp，编码314个氨基酸残基，大小约33.27 kD，含有N-端信号肽，预测为胞外分泌蛋白；FocChp基因的cDNA编码区全长1302 bp，编码433个氨基酸残基，大小约49.17 k D，亚细胞定位在细胞核，预测为含有3个锌指结构域的转录因子；FocGf12基因的cDNA编码区全长1533 bp，编码480个氨基酸残基，大小约53.54 kD，该蛋白存在跨膜结构域，具有GPI修饰位点，亚细胞定位在胞外，预测为糖苷水解酶家族12蛋白。【结论】通过GWAS获得香蕉枯萎病菌3个致病相关基因FocScp、FocChp和FocGhf12，其编码蛋白分别为分泌蛋白、转录因子和结构蛋白。
Keyword ：
全基因组关联分析 全基因组关联分析 生物信息学分析 生物信息学分析 致病相关基因 致病相关基因 香蕉枯萎病菌 香蕉枯萎病菌
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| GB/T 7714 | 高辉 , 蒋尚伯 , 杨迪 et al. 基于全基因组关联分析的香蕉枯萎病菌致病基因挖掘与功能研究 [J]. | 南方农业学报 , 2024 , 55 (08) : 2442-2453 . |
| MLA | 高辉 et al. "基于全基因组关联分析的香蕉枯萎病菌致病基因挖掘与功能研究" . | 南方农业学报 55 . 08 (2024) : 2442-2453 . |
| APA | 高辉 , 蒋尚伯 , 杨迪 , 杜婵娟 , 张晋 , 潘连富 et al. 基于全基因组关联分析的香蕉枯萎病菌致病基因挖掘与功能研究 . | 南方农业学报 , 2024 , 55 (08) , 2442-2453 . |
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Arabidopsis PHYTOALEXIN DEFICIENT 4 (PAD4) has an essential role in pathogen resistance as a heterodimer with ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1). Here we investigated an additional PAD4 role in which it associates with and promotes the maturation of the immune-related cysteine protease RESPONSIVE TO DEHYDRATION 19 (RD19). We found that RD19 and its paralog RD19c promoted EDS1- and PAD4-mediated effector-triggered immunity to an avirulent Pseudomonas syringae strain, DC3000, expressing the effector AvrRps4 and basal immunity against the fungal pathogen Golovinomyces cichoracearum. Overexpression of RD19, but not RD19 protease-inactive catalytic mutants, in Arabidopsis transgenic lines caused EDS1- and PAD4-dependent autoimmunity and enhanced pathogen resistance. In these lines, RD19 maturation to a pro-form required its catalytic residues, suggesting that RD19 undergoes auto-processing. In transient assays, PAD4 interacted preferentially with the RD19 pro-protease and promoted its nuclear accumulation in leaf cells. Our results lead us to propose a model for PAD4-stimulated defense potentiation. PAD4 promotes maturation and nuclear accumulation of processed RD19, and RD19 then stimulates EDS1-PAD4 dimer activity to confer pathogen resistance. This study highlights potentially important additional PAD4 functions that eventually converge on canonical EDS1-PAD4 dimer signaling in plant immunity. Lipase-like immune signaling protein PAD4 promotes the maturation and nuclear accumulation of a cysteine protease and enhances disease resistance to biotrophic pathogens in Arabidopsis.
Keyword ：
Cysteine protease Cysteine protease EDS1 EDS1 effector triggered immunity effector triggered immunity nucleus nucleus PAD4 PAD4 resistance resistance
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| GB/T 7714 | Zeng, Yanhong , Zheng, Zichao , Hessler, Giuliana et al. Arabidopsis PHYTOALEXIN DEFICIENT 4 promotes the maturation and nuclear accumulation of immune-related cysteine protease RD19 [J]. | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 75 (5) : 1530-1546 . |
| MLA | Zeng, Yanhong et al. "Arabidopsis PHYTOALEXIN DEFICIENT 4 promotes the maturation and nuclear accumulation of immune-related cysteine protease RD19" . | JOURNAL OF EXPERIMENTAL BOTANY 75 . 5 (2023) : 1530-1546 . |
| APA | Zeng, Yanhong , Zheng, Zichao , Hessler, Giuliana , Zou, Ke , Leng, Junchen , Bautor, Jaqueline et al. Arabidopsis PHYTOALEXIN DEFICIENT 4 promotes the maturation and nuclear accumulation of immune-related cysteine protease RD19 . | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 75 (5) , 1530-1546 . |
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Plant trichomes are a specialized cellular tissue that functions in resistance to biotic , abiotic stresses. In rice, three transcription-factor genes: OsWOX3B, HL6, OsSPL10, have been found to control tri-chome development. Although studies have shown interactions between the three genes, their full rela-tionship in trichome development is unclear. We found that the expression levels of OsWOX3B and HL6 were both reduced in OsSPL10-knockout plants but increased in OsSPL10-overexpression plants, suggest-ing that OsSPL10 positively regulates their expression. Physical interaction between OsSPL10 and OsWOX3B was found both in vivo and in vitro and attenuated their abilities to bind to the promoter of HL6 to activate its transcription. This mechanism may regulate trichome length by adjusting the expres-sion of HL6. A rice gene network regulating trichome development is proposed. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keyword ：
HL6 HL6 Interaction Interaction OsSPL10 OsSPL10 OsWOX3B OsWOX3B Rice Rice Trichome Trichome
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| GB/T 7714 | Liao, Qiaoling , Cheng, Xinle , Lan, Tong et al. OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.) [J]. | CROP JOURNAL , 2023 , 11 (6) : 1711-1718 . |
| MLA | Liao, Qiaoling et al. "OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.)" . | CROP JOURNAL 11 . 6 (2023) : 1711-1718 . |
| APA | Liao, Qiaoling , Cheng, Xinle , Lan, Tong , Guo, Xiaokuan , Su, Zilong , An, Xiaoxiao et al. OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.) . | CROP JOURNAL , 2023 , 11 (6) , 1711-1718 . |
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A signaling pathway to accommodate arbuscular mycorrhizal fungi consists of receptor-like kinase-associated components at the periarbuscular membrane and is evolutionarily conserved in legumes and cereals. Arbuscular mycorrhizal (AM) symbiosis is a widespread, ancient mutualistic association between plants and fungi, and facilitates nutrient uptake into plants. Cell surface receptor-like kinases (RLKs) and receptor-like cytoplasmic kinases (RLCKs) play pivotal roles in transmembrane signaling, while few RLCKs are known to function in AM symbiosis. Here, we show that 27 out of 40 AM-induced kinases (AMKs) are transcriptionally upregulated by key AM transcription factors in Lotus japonicus. Nine AMKs are only conserved in AM-host lineages, among which the SPARK-RLK-encoding gene KINASE3 (KIN3) and the RLCK paralogues AMK8 and AMK24 are required for AM symbiosis. KIN3 expression is directly regulated by the AP2 transcription factor CTTC MOTIF-BINDING TRANSCRIPTION FACTOR1 (CBX1), which regulates the reciprocal exchange of nutrients in AM symbiosis, via the AW-box motif in the KIN3 promoter. Loss of function mutations in KIN3, AMK8, or AMK24 result in reduced mycorrhizal colonization in L. japonicus. AMK8 and AMK24 physically interact with KIN3. KIN3 and AMK24 are active kinases and AMK24 directly phosphorylates KIN3 in vitro. Moreover, CRISPR-Cas9-mediated mutagenesis of OsRLCK171, the sole homolog of AMK8 and AMK24 in rice (Oryza sativa), leads to diminished mycorrhization with stunted arbuscules. Overall, our results reveal a crucial role of the CBX1-driven RLK/RLCK complex in the evolutionarily conserved signaling pathway enabling arbuscule formation.
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| GB/T 7714 | Leng, Junchen , Wei, Xiaotong , Jin, Xinyi et al. ARBUSCULAR MYCORRHIZA-INDUCED KINASES AMK8 and AMK24 associate with the receptor-like kinase KINASE3 to regulate arbuscular mycorrhizal symbiosis in Lotus japonicus [J]. | PLANT CELL , 2023 , 35 (6) : 2006-2026 . |
| MLA | Leng, Junchen et al. "ARBUSCULAR MYCORRHIZA-INDUCED KINASES AMK8 and AMK24 associate with the receptor-like kinase KINASE3 to regulate arbuscular mycorrhizal symbiosis in Lotus japonicus" . | PLANT CELL 35 . 6 (2023) : 2006-2026 . |
| APA | Leng, Junchen , Wei, Xiaotong , Jin, Xinyi , Wang, Longxiang , Fan, Kai , Zou, Ke et al. ARBUSCULAR MYCORRHIZA-INDUCED KINASES AMK8 and AMK24 associate with the receptor-like kinase KINASE3 to regulate arbuscular mycorrhizal symbiosis in Lotus japonicus . | PLANT CELL , 2023 , 35 (6) , 2006-2026 . |
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本发明公开了一种香蕉原生质体的制备方法和应用，属于植物原生质体技术领域。本发明采用香蕉吸芽的内层组织，改进酶解溶液配方，通过多次低速离心与重悬的方法，获得高纯度香蕉原生质体，达到每克组织1.22×107个原生质体。本发明具有较高重现性，可稳定获得高质量香蕉原生质体，实现转化效率达到75%以上的效果。本发明为香蕉蛋白质亚细胞定位、启动子功能验证等分子生物学研究提供了有效的研究技术，具有广泛应用价值。
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| GB/T 7714 | 崔海涛 , 赵春慧 , 付岗 et al. 一种香蕉原生质体的制备方法和应用 : CN202211050174.X[P]. | 2022-08-31 . |
| MLA | 崔海涛 et al. "一种香蕉原生质体的制备方法和应用" : CN202211050174.X. | 2022-08-31 . |
| APA | 崔海涛 , 赵春慧 , 付岗 , 王衍坤 , 李舒宇 , 杨迪 et al. 一种香蕉原生质体的制备方法和应用 : CN202211050174.X. | 2022-08-31 . |
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本发明涉及植物分子生物学领域，具体而言本发明涉及植物COP1基因突变体及其促进植物早花的用途。在拟南芥COP1蛋白质中以S648为中心5埃范围内立体结构上靠近的16个氨基酸中，一个或2个或更多个氨基酸缺失、替换或插入影响COP1与CO蛋白质的相互作用，导致CO蛋白质不被降解，拟南芥植物发生早花。并且该16个氨基酸在诸多植物物种中高度保守。因此本发明的COP1基因变体具有广泛的应用价值，可以使用诸多植物物种的COP1基因变体培育具有早花特性的诸多植物品种。
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| GB/T 7714 | 胡金勇 , 于冬梅 , 董雪 et al. 促进植物开花的COP1基因突变及其用途 : CN202211226069.7[P]. | 2022-10-09 . |
| MLA | 胡金勇 et al. "促进植物开花的COP1基因突变及其用途" : CN202211226069.7. | 2022-10-09 . |
| APA | 胡金勇 , 于冬梅 , 董雪 , 崔海涛 , 蒋晓东 , 邹可 et al. 促进植物开花的COP1基因突变及其用途 : CN202211226069.7. | 2022-10-09 . |
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Plant cell surface and intracellular immune receptors recognizing pathogen attack utilize the same defense machineries to mobilize resistance. New genetic, protein structural and biochemical information on receptor activation and signaling is transforming understanding of how their shared defense network operates. We discuss the biochemical activities of two classes of intracellular nucleotide-binding/leucine-rich repeat (NLR) receptor - one forming a Ca2+ channel, the other an NADase enzyme - which define engagement of enhanced disease susceptibility 1 (EDS1)-family heterodimers and cofunctioning helper NLRs (RNLs) to connect receptor systems and amplify defenses. Toll-interleukin-1 receptor (TIR) domain NLR receptors and TIR-domain proteins, with a capacity to produce NAD+-derived small molecules, require EDS1 dimers and RNLs for defense induction. The TIR-driven EDS1/RNL modules emerge as central elements in Ca2+-based immunity signaling initiated by receptors outside and inside host cells.
Keyword ：
disease resistance disease resistance EDS1 EDS1 effector-triggered immunity effector-triggered immunity immune receptor immune receptor NADase NADase pattern-triggered immunity pattern-triggered immunity RNL RNL TIR TIR
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| GB/T 7714 | Parker, Jane E. , Hessler, Giuliana , Cui, Haitao . A new biochemistry connecting pathogen detection to induced defense in plants [J]. | NEW PHYTOLOGIST , 2022 , 234 (3) : 819-826 . |
| MLA | Parker, Jane E. et al. "A new biochemistry connecting pathogen detection to induced defense in plants" . | NEW PHYTOLOGIST 234 . 3 (2022) : 819-826 . |
| APA | Parker, Jane E. , Hessler, Giuliana , Cui, Haitao . A new biochemistry connecting pathogen detection to induced defense in plants . | NEW PHYTOLOGIST , 2022 , 234 (3) , 819-826 . |
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The breeding of triploid banana cultivars with improved traits, such as yield and disease resistance, remains a major challenge for breeders. One reason is that the molecular study and functional gene analysis in bananas fall behind due to the difficulties of its genetic manipulation. The plant protoplast-based transient transformation has been documented and widely used as a versatile and convenient system for functional gene analysis in many plant species. However, an efficient high-quality protoplast isolation and transformation system is still lacking for bananas. Here, we established an efficient protoplast isolation and transformation method for bananas by selecting proper source materials, optimizing conditions for enzymatic hydrolysis and PEG-mediated transfection. We found the best source materials for banana protoplasts' isolation are young suckers, which give a yield of protoplasts ranging from 2.5 x 10(6) to 10.1 x 10(7) g(-1) fresh weight after 5 to 6 h of enzymolysis. The yield is sufficient for most assays that have been established in protoplasts-based systems, such as protein subcellular localization and protein interaction assays. Moreover, using the established transient gene expression system in banana protoplasts, we validated the subcellular localization of Arabidopsis VESICLE SORTING RECEPTOR 1 (VSR1) and the protein self-interaction of Arabidopsis CNGC20 on the cell membrane. The results indicated this system works well and could be routinely used for the functional characterization of banana genes.
Keyword ：
banana banana Musa spp. Musa spp. protoplast isolation protoplast isolation transformation transformation transient expression transient expression
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| GB/T 7714 | Zhao, Chunhui , Li, Shuyu , Du, Chanjuan et al. Establishment of a Protoplasts-Based Transient Expression System in Banana (Musa spp.) [J]. | AGRONOMY-BASEL , 2022 , 12 (11) . |
| MLA | Zhao, Chunhui et al. "Establishment of a Protoplasts-Based Transient Expression System in Banana (Musa spp.)" . | AGRONOMY-BASEL 12 . 11 (2022) . |
| APA | Zhao, Chunhui , Li, Shuyu , Du, Chanjuan , Gao, Hui , Yang, Di , Fu, Gang et al. Establishment of a Protoplasts-Based Transient Expression System in Banana (Musa spp.) . | AGRONOMY-BASEL , 2022 , 12 (11) . |
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