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学者姓名：陈阿海
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Abstract ：
center dot Eukaryotes have evolved sophisticated post-translational modifications to regulate protein function and numerous biological processes, including ubiquitination controlled by the coordinated action of ubiquitin-conjugating enzymes and deubiquitinating enzymes (Dubs). However, the function of deubiquitination in pathogenic fungi is largely unknown. center dot Here, the distribution of Dubs in the fungal kingdom was surveyed and their functions were systematically characterized using the phytopathogen Fusarium graminearum as the model species, which causes devastating diseases of all cereal species world-wide. center dot Our findings demonstrate that Dubs are critical for fungal development and virulence, especially the ubiquitin-specific protease 15 (Ubp15). Global ubiquitome analysis and subsequent experiments identified three important substrates of Ubp15, including the autophagy-related protein Atg8, the mitogen-activated protein kinase Gpmk1, and the mycotoxin deoxynivalenol (DON) biosynthetic protein Tri4. Ubp15 regulates the deubiquitination of the Atg8, thereby impacting its subcellular localization and the autophagy process. Moreover, Ubp15 also modulates the deubiquitination of Gpmk1 and Tri4. This modulation subsequently influences their protein stabilities and further affects the formation of penetration structures and the biosynthetic process of DON, respectively. center dot Collectively, our findings reveal a previously unknown regulatory pathway of a deubiquitinating enzyme for fungal virulence and highlight the potential of Ubp15 as a target for combating fungal diseases.
Keyword ：
deubiquitinating enzyme deubiquitinating enzyme post-translational modification post-translational modification protein stability protein stability ubiquitination ubiquitination virulence virulence wheat-Fusarium graminearum interaction wheat-Fusarium graminearum interaction
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| GB/T 7714 | Chen, Ahai , Han, Xingmin , Liu, Chao et al. Profiling of deubiquitinases that control virulence in the pathogenic plant fungus Fusarium graminearum [J]. | NEW PHYTOLOGIST , 2024 , 242 (1) : 192-210 . |
| MLA | Chen, Ahai et al. "Profiling of deubiquitinases that control virulence in the pathogenic plant fungus Fusarium graminearum" . | NEW PHYTOLOGIST 242 . 1 (2024) : 192-210 . |
| APA | Chen, Ahai , Han, Xingmin , Liu, Chao , Zhou, Yifan , Ren, Yiyi , Shen, Xingxing et al. Profiling of deubiquitinases that control virulence in the pathogenic plant fungus Fusarium graminearum . | NEW PHYTOLOGIST , 2024 , 242 (1) , 192-210 . |
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Ubiquitinated biosynthetic and surface proteins destined for degradation are sorted into the lysosome/vacuole via the multivesicular body sorting pathway, which depends on the function of ESCRT machinery. Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most devastating diseases for wheat and barley worldwide. To better understand the role of ESCRT machinery in F. graminearum, we investigated the function of ESCRT-III accessory proteins FgVps60, FgDid2 and FgIst1 in this study. FgVps60-GFP, FgDid2-GFP and FgIst1-GFP are localized to punctate structures adjacent to the vacuolar membrane except for FgIst1-GFP that is also found in the nucleus. Then, the gene deletion mutants Delta Fgvps60, Delta Fgdid2 and Delta Fgist1 displayed defective growth to a different extent. Delta Fgvps60 and Delta Fgdid2 but not Delta Fgist1 also showed significant reduction in hydrophobicity on cell surface, conidiation, perithecia production and virulence. Interestingly, Delta Fgist1 mutant produced a significantly higher level of DON while showing a minor reduction in pathogenicity. Microscopic analyses revealed that FgVps60 but not FgIst1 and FgDid2 is necessary for endocytosis. Moreover, spontaneous mutations were identified in the Delta Fgvps60 mutant that partially rescued its defects in growth and conidiation. Taken together, we conclude that ESCRT-III accessory proteins play critical roles in growth, reproduction and plant infection in F. graminearum.
Keyword ：
DON production DON production Endocytosis Endocytosis ESCRT-III accessory proteins ESCRT-III accessory proteins Fusarium graminearum Fusarium graminearum Pathogenicity Pathogenicity
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| GB/T 7714 | Xie, Qiurong , Chen, Ahai , Zhang, Yunzhi et al. ESCRT-III accessory proteins regulate fungal development and plant infection in Fusarium graminearum [J]. | CURRENT GENETICS , 2019 , 65 (4) : 1041-1055 . |
| MLA | Xie, Qiurong et al. "ESCRT-III accessory proteins regulate fungal development and plant infection in Fusarium graminearum" . | CURRENT GENETICS 65 . 4 (2019) : 1041-1055 . |
| APA | Xie, Qiurong , Chen, Ahai , Zhang, Yunzhi , Zhang, Chengkang , Hu, Yanpei , Luo, Zenghong et al. ESCRT-III accessory proteins regulate fungal development and plant infection in Fusarium graminearum . | CURRENT GENETICS , 2019 , 65 (4) , 1041-1055 . |
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Multivesicular bodies (MVBs) are critical intermediates in the trafficking of ubiquitinated endocytosed surface proteins to the lysosome/vacuole for destruction. Recognizing and packaging ubiquitin modified cargoes to the MVB pathway require ESCRT (Endosomal sorting complexes required for transport) machinery, which consists of four core subcomplexes, ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III. Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. Our previous results showed that ESCRT-0 is essential for fungal development and pathogenicity in Fusarium graminearum. We then, in this study, systemically studied the protein-protein interactions within F graminearum ESCRT-I, -II or -III complex, as well as between ESCRT-0 and ESCRT-I, ESCRT-I and ESCRT-II, and ESCRT-II and ESCRT-III complexes and found that loss of any ESCRT component resulted in abnormal function in endocytosis. In addition, ESCRT deletion mutants displayed severe defects in growth, deoxynivalenol (DON) production, virulence, sexual, and asexual reproduction. Importantly genetic complementation with corresponding ESCRT genes fully rescued all these defective phenotypes, indicating the essential role of ESCRT machinery in fungal development and plant infection in F graminearum. Taken together, the protein-protein interactome and biological functions of the ESCRT machinery is first profoundly characterized in F. graminearum, providing a foundation for further exploration of ESCRT machinery in filamentous fungi.
Keyword ：
endocytosis endocytosis ESCRT complexes ESCRT complexes Fusarium graminearum Fusarium graminearum interactome interactome pathogenicity pathogenicity
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| GB/T 7714 | Xie, Qiurong , Chen, Ahai , Zhang, Yunzhi et al. Component Interaction of ESCRT Complexes Is Essential for Endocytosis-Dependent Growth, Reproduction, DON Production and Full Virulence in Fusarium graminearum [J]. | FRONTIERS IN MICROBIOLOGY , 2019 , 10 . |
| MLA | Xie, Qiurong et al. "Component Interaction of ESCRT Complexes Is Essential for Endocytosis-Dependent Growth, Reproduction, DON Production and Full Virulence in Fusarium graminearum" . | FRONTIERS IN MICROBIOLOGY 10 (2019) . |
| APA | Xie, Qiurong , Chen, Ahai , Zhang, Yunzhi , Yuan, Mingyue , Xie, Wei , Zhang, Chengkang et al. Component Interaction of ESCRT Complexes Is Essential for Endocytosis-Dependent Growth, Reproduction, DON Production and Full Virulence in Fusarium graminearum . | FRONTIERS IN MICROBIOLOGY , 2019 , 10 . |
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Circadian clock mechanisms have been extensively investigated but the main rate-limiting step that determines circadian period remains unclear. Formation of a stable complex between clock proteins and CK1 is a conserved feature in eukaryotic circadian mechanisms. Here we show that the FRQ-CK1 interaction, but not FRQ stability, correlates with circadian period in Neurospora circadian clock mutants. Mutations that specifically affect the FRQ-CK1 interaction lead to severe alterations in circadian period. The FRQ-CK1 interaction has two roles in the circadian negative feedback loop. First, it determines the FRQ phosphorylation profile, which regulates FRQ stability and also feeds back to either promote or reduce the interaction itself. Second, it determines the efficiency of circadian negative feedback process by mediating FRQ-dependent WC phosphorylation. Our conclusions are further supported by mathematical modeling and in silico experiments. Together, these results suggest that the FRQ-CK1 interaction is a major rate-limiting step in circadian period determination.
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| GB/T 7714 | Liu, Xiao , Chen, Ahai , Caicedo-Casso, Angelica et al. FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora [J]. | NATURE COMMUNICATIONS , 2019 , 10 . |
| MLA | Liu, Xiao et al. "FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora" . | NATURE COMMUNICATIONS 10 (2019) . |
| APA | Liu, Xiao , Chen, Ahai , Caicedo-Casso, Angelica , Cui, Guofei , Du, Mingjian , He, Qun et al. FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora . | NATURE COMMUNICATIONS , 2019 , 10 . |
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In eukaryotes, the retromer is an endosome-localized complex involved in protein retrograde transport. However, the role of such intracellular trafficking events in pathogenic fungal development and pathogenicity remains unclear. The role of the retromer complex in Fusarium graminearum was investigated using cell biological and genetic methods. We observed the retromer core component FgVps35 (Vacuolar Protein Sorting 35) in the cytoplasm as fast-moving puncta. FgVps35-GFP co-localized with both early and late endosomes, and associated with the trans-Golgi network (TGN), suggesting that FgVps35 functions at the donor endosome membrane to mediate TGN trafficking. Disruption of microtubules with nocodazole significantly restricted the transportation of FgVps35-GFP and resulted in severe germination and growth defects. Mutation of FgVPS35 not only mimicked growth defects induced by pharmacological treatment, but also affected conidiation, ascospore formation and pathogenicity. Using yeast two-hybrid assays, we determined the interactions among FgVps35, FgVps26, FgVps29, FgVps17 and FgVps5 which are analogous to the yeast retromer complex components. Deletion of any one of these genes resulted in similar phenotypic defects to those of the Delta Fgvps35 mutant and disrupted the stability of the complex. Overall, our results provide the first clear evidence of linkage between the retrograde transport mediated by the retromer complex and virulence in F. graminearum.
Keyword ：
FgVps35 FgVps35 Fusarium graminearum Fusarium graminearum pathogenicity pathogenicity retrograde trafficking retrograde trafficking retromer retromer
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| GB/T 7714 | Zheng, Wenhui , Zheng, Huawei , Zhao, Xu et al. Retrograde trafficking from the endosome to the trans-Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum [J]. | NEW PHYTOLOGIST , 2016 , 210 (4) : 1327-1343 . |
| MLA | Zheng, Wenhui et al. "Retrograde trafficking from the endosome to the trans-Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum" . | NEW PHYTOLOGIST 210 . 4 (2016) : 1327-1343 . |
| APA | Zheng, Wenhui , Zheng, Huawei , Zhao, Xu , Zhang, Ying , Xie, Qiurong , Lin, Xiaolian et al. Retrograde trafficking from the endosome to the trans-Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum . | NEW PHYTOLOGIST , 2016 , 210 (4) , 1327-1343 . |
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Septins are GTP-binding proteins that regulate cell polarity, cytokinesis and cell morphogenesis. Fusarium head blight (FHB), caused by Fusarium graminearum, is one of the most devastating diseases worldwide. In this study, we have functionally characterized the core septins, Cdc3, Cdc10, Cdc11 and Cdc12 in F. graminearum. The loss of FgCdc3, FgCdc11, FgCdc12, but not FgCdc10, mutants showed significant reduction in growth, conidiation and virulence. Microscopic analyses revealed that all of them were involved in septum formation and nuclear division. Moreover, disruption of septin genes resulted in morphological defects in ascospores and conidia. Interestingly, conidia produced by Delta Fgcdc3, Delta Fgcdc11 and Delta Fgcdc12 mutants exhibited deformation with interconnecting conidia in contrast to their parent wild-type strain PH-1 and the Delta Fgcdc10 mutant that produced normal conidia. Using yeast two-hybrid assays, we determined the interactions among FgCdc3, FgCdc10, FgCdc11 and FgCdc12. Taken together, our results indicate that septins play important roles in the nuclear division, morphogenesis and pathogenicity in F. graminearum. (C) 2016 Elsevier Inc. All rights reserved.
Keyword ：
Fusarium graminearum Fusarium graminearum Morphogenesis Morphogenesis Nuclear division Nuclear division Pathogenicity Pathogenicity Septin Septin Septum formation Septum formation
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| GB/T 7714 | Chen, Ahai , Xie, Qiurong , Lin, Yahong et al. Septins are involved in nuclear division, morphogenesis and pathogenicity in Fusarium graminearum [J]. | FUNGAL GENETICS AND BIOLOGY , 2016 , 94 : 79-87 . |
| MLA | Chen, Ahai et al. "Septins are involved in nuclear division, morphogenesis and pathogenicity in Fusarium graminearum" . | FUNGAL GENETICS AND BIOLOGY 94 (2016) : 79-87 . |
| APA | Chen, Ahai , Xie, Qiurong , Lin, Yahong , Xu, Huaijian , Shang, Wenjie , Zhang, Jun et al. Septins are involved in nuclear division, morphogenesis and pathogenicity in Fusarium graminearum . | FUNGAL GENETICS AND BIOLOGY , 2016 , 94 , 79-87 . |
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Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The vacuolar protein sorting (Vps) protein Vps27 is a component of ESCRT-0 involved in the multivesicular body (MVB) sorting pathway during endocytosis. In this study, we investigated the function of FgVps27 using a gene replacement strategy. The FgVPS27 deletion mutant (Delta Fgvps27) exhibited a reduction in growth rate, aerial hyphae formation and hydrophobicity. It also showed increased sensitivity to cell wall-damaging agents and to osmotic stresses. In addition, FgHog1, the critical component of high osmolarity glycerol response pathway, was mis-localized in the Delta Fgvps27 mutant upon NaCl treatment. Furthermore, the Delta Fgvps27 mutant was defective in conidial production and was unable to generate perithecium in sexual reproduction. The depletion of FgVPS27 also caused a significant reduction in virulence. Further analysis by domain-specific deletion revealed that the FYVE domain was essential for the FgVps27 function and was necessary for the proper localization of FgVps27-GFP and endocytosis. Another component of ESCRT-0, the FgVps27-interacting partner FgHse1, also played an important role in F. graminearum development and pathogenesis. Overall, our results indicate that ESCRT-0 components play critical roles in a variety of cellular and biological processes.
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| GB/T 7714 | Xie, Qiurong , Chen, Ahai , Zheng, Wenhui et al. Endosomal sorting complexes required for transport-0 is essential for fungal development and pathogenicity in Fusarium graminearum [J]. | ENVIRONMENTAL MICROBIOLOGY , 2016 , 18 (11) : 3742-3757 . |
| MLA | Xie, Qiurong et al. "Endosomal sorting complexes required for transport-0 is essential for fungal development and pathogenicity in Fusarium graminearum" . | ENVIRONMENTAL MICROBIOLOGY 18 . 11 (2016) : 3742-3757 . |
| APA | Xie, Qiurong , Chen, Ahai , Zheng, Wenhui , Xu, Huaijian , Shang, Wenjie , Zheng, Huawei et al. Endosomal sorting complexes required for transport-0 is essential for fungal development and pathogenicity in Fusarium graminearum . | ENVIRONMENTAL MICROBIOLOGY , 2016 , 18 (11) , 3742-3757 . |
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禾谷镰刀菌是引起小麦赤霉病的主要病原真菌。分生孢子在侵染过程中起关键作用。先前研究表明同源异型盒(Homeobox)转录因子FgHtf1特异调控分生孢子的产生。为明确其调控产孢的分子机制,本研究采用高通量RNA测序技术(RNA-Seq),对ΔFghtf1突变体菌株和野生型PH-1菌株进行测序,结果显示其中591个基因转录上调,549个基因下调。对这些基因进行功能预测并聚类分析发现:上下调基因中分别有约30%和22%参与新陈代谢和能量转运。运用荧光定量PCR,检测10个基因的表达,验证了RNA-Seq的结果是可靠的。运用同源重组方法敲除明显下调的基因FGSG_10565和FGSG_07629,表型分析结果显示,相比野生型,ΔFGSG_10565和ΔFGSG_07629的生长速率和产孢量均不变,说明两个基因的表达受FgHtf1调控,但功能上不直接参与分生孢子的形成。
Keyword ：
RNA-Seq RNA-Seq 产孢 产孢 同源异型盒基因 同源异型盒基因 基因差异表达 基因差异表达 禾谷镰刀菌 禾谷镰刀菌
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| GB/T 7714 | 张红红 , 郑文辉 , 骆琼 et al. 禾谷镰刀菌Homeobox转录因子FgHtf1敲除突变体基因差异表达分析 [J]. | 中国科技论文 , 2015 , 10 (12) : 1392-1397 . |
| MLA | 张红红 et al. "禾谷镰刀菌Homeobox转录因子FgHtf1敲除突变体基因差异表达分析" . | 中国科技论文 10 . 12 (2015) : 1392-1397 . |
| APA | 张红红 , 郑文辉 , 骆琼 , 乔向威 , 陈阿海 , 余文英 et al. 禾谷镰刀菌Homeobox转录因子FgHtf1敲除突变体基因差异表达分析 . | 中国科技论文 , 2015 , 10 (12) , 1392-1397 . |
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The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, the role of such trafficking events during pathogen-host interaction remains unclear. Here, we report that the cargo-recognition complex (MoVps35, MoVps26 and MoVps29) of the retromer is essential for appressorium-mediated host penetration by Magnaporthe oryzae, the causal pathogen of the blast disease in rice. Loss of retromer function blocked glycogen distribution and turnover of lipid bodies, delayed nuclear degeneration and reduced turgor during appressorial development. Cytological observation revealed dynamic MoVps35-GFP foci co-localized with autophagy-related protein RFP-MoAtg8 at the periphery of autolysosomes. Furthermore, RFP-MoAtg8 interacted with MoVps35-GFP in vivo, RFP-MoAtg8 was mislocalized to the vacuole and failed to recycle from the autolysosome in the absence of the retromer function, leading to impaired biogenesis of autophagosomes. We therefore conclude that retromer is essential for autophagy-dependent plant infection by the rice blast fungus.
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| GB/T 7714 | Zheng, Wenhui , Zhou, Jie , He, Yunlong et al. Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus [J]. | PLOS GENETICS , 2015 , 11 (12) . |
| MLA | Zheng, Wenhui et al. "Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus" . | PLOS GENETICS 11 . 12 (2015) . |
| APA | Zheng, Wenhui , Zhou, Jie , He, Yunlong , Xie, Qiurong , Chen, Ahai , Zheng, Huawei et al. Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus . | PLOS GENETICS , 2015 , 11 (12) . |
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<正>赤霉病是在世界范围内多种作物上反复发生的病害,小麦受害尤其严重。禾谷镰刀菌是该病最主要的病原。小麦染病后,会造成产量的严重下降,同时还会产生多种真菌毒素,对人和动物的健康造成极大的威胁。
Keyword ：
CDC42 CDC42 基因敲除 基因敲除 禾谷镰刀菌 禾谷镰刀菌 赤霉病 赤霉病
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| GB/T 7714 | 张承康 , 陈阿海 , 黄德建 et al. 禾谷镰刀菌中一个假定的Cdc42基因的功能分析 [C] //中国植物病理学会2010年学术年会论文集 . 2010 . |
| MLA | 张承康 et al. "禾谷镰刀菌中一个假定的Cdc42基因的功能分析" 中国植物病理学会2010年学术年会论文集 . (2010) . |
| APA | 张承康 , 陈阿海 , 黄德建 , 王宗华 . 禾谷镰刀菌中一个假定的Cdc42基因的功能分析 中国植物病理学会2010年学术年会论文集 . (2010) . |
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