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学者姓名：苏金为
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Abstract ：
Poly(procaterol hydrochloride) (p-ProH) polymeric film was successfully deposited onto the carboxyl multi-walled carbon nanotube (CMWCNT) modified glass carbon electrode (GCE) to construct a p-ProH/CMWCNT composite modified GCE. Due to the synergistic effect of p-ProH and CMWCNT in the composite, the developed sensor can enormously enhance the oxidation peak current of bromhexine hydrochloride (BrH) at ca. + 0.90 V. Based on this appearance, an electrochemical method was established for the sensitive and selective determination of BrH with differential pulse voltammetry (DPV). Various conditions affecting the peak current response of BrH were studied and optimized. Under the best conditions, the oxidation peak current of BrH is linear to its concentration in two linear dynamic ranges of 0.2-1.0 mol L-1 (R = 0.9948) and 1.0-8.0 mol L-1 (R = 0.9956), with a detection limit of 0.1 mol L-1 (S/N = 3). Interference experiment indicated that the as-prepared electrochemical sensor showed wonderful selectivity to the recognition of BrH and was free from disturbance of many other electro-active substances such as dopamine, ascorbic and uric acid. Finally, the practicability of the BrH sensor was verified by the satisfactory results acquired from the BrH determination in pharmaceutical preparation and human serum.
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| GB/T 7714 | Kong, Dexian , Han, Libin , Wang, Zeming et al. An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride [J]. | RSC ADVANCES , 2019 , 9 (21) : 11901-11911 . |
| MLA | Kong, Dexian et al. "An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride" . | RSC ADVANCES 9 . 21 (2019) : 11901-11911 . |
| APA | Kong, Dexian , Han, Libin , Wang, Zeming , Jiang, Lili , Zhang, Qian , Wu, Qiong et al. An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride . | RSC ADVANCES , 2019 , 9 (21) , 11901-11911 . |
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Clean fuels with extremely low sulfur content are highly desirable due to environmental concerns. Herein, three water-stable and eco-friendly metal organic frameworks with tunable window diameters, denoted as MOF-808X, have been employed as PTA solid supports. An array of PTA@MOF-808X composites were facilely synthesized via the encapsulation strategy. With tunable window sizes and adjustable PTA loading amounts, the obtained PTA@MOF-808X composites were screened for catalytic oxidative desulfurization (ODS) with H2O2 serving as oxidant. The experiments found that 42% PTA@MOF-808A had the highest catalytic ODS activity and could completely remove dibenzothiophene (DBT) in a model fuel with an initial sulfur content of 1000 ppm within 30 min, which falls far below the acceptable limits for fuel standards (10 ppm). Further investigations revealed that this high catalytic activity could be attributed to the cooperative catalysis of metal clusters in the host framework and the guest PTA molecules. Moreover, 42%PTA@MOF-808A could be facilely recovered and reused for at least five runs without loss of catalytic activity. Having a combination of eco-sustainability, high stability, high catalytic activity, and good recyclability, 42%PTA@MOF-808A therefore represents a new benchmark material for catalytic ODS and provides a new perspective for ultradeep desulfurization.
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| GB/T 7714 | Lin, Zu-Jin , Zheng, He-Qi , Chen, Jin et al. Encapsulation of Phosphotungstic Acid into Metal-Organic Frameworks with Tunable Window Sizes: Screening of PTA@MOF Catalysts for Efficient Oxidative Desulfurization [J]. | INORGANIC CHEMISTRY , 2018 , 57 (20) : 13009-13019 . |
| MLA | Lin, Zu-Jin et al. "Encapsulation of Phosphotungstic Acid into Metal-Organic Frameworks with Tunable Window Sizes: Screening of PTA@MOF Catalysts for Efficient Oxidative Desulfurization" . | INORGANIC CHEMISTRY 57 . 20 (2018) : 13009-13019 . |
| APA | Lin, Zu-Jin , Zheng, He-Qi , Chen, Jin , Zhuang, Wan-E , Lin, Yue-Xu , Su, Jin-Wei et al. Encapsulation of Phosphotungstic Acid into Metal-Organic Frameworks with Tunable Window Sizes: Screening of PTA@MOF Catalysts for Efficient Oxidative Desulfurization . | INORGANIC CHEMISTRY , 2018 , 57 (20) , 13009-13019 . |
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In the present study, procaterol hydrochloride (ProH) was successfully electropolymerized onto a glass carbon electrode (GCE) with simply cyclic voltammetry scans to construct a poly(procaterol hydrochloride) (p-ProH) membrane modified electrode. Compared with the bare GCE, much higher oxidation peak current responses and better peak potentials separation could be obtained for the simultaneous oxidation of dopamine (DA) and uric acid (UA), owning to the excellent electrocatalytic ability of the p-ProH membrane. And it's based on that a square wave voltammetry (SWV) method was developed to selective and simultaneous measurement of DA and UA. Under the optimum conditions, the linear dependence of oxidation peak current on analyte concentrations were found to be 1.0-100 mu mol/L and 2-100 mu mol/L, giving detection limits of 0.3 mu mol/L and 0.5 mu mol/L for DA and UA, separately. The as prepared modified electrode shows simplicity in construction with the merits of good reproducibility, high stability, passable selectivity and nice sensitivity. Finally, the proposed p-ProH membrane modified electrode was successfully devoted to the detection of DA and UA in biological fluids such as human serum and urine with acceptable results.
Keyword ：
Biological samples Biological samples Dopamine Dopamine Electrochemical Electrochemical Poly(procaterol hydrochloride) Poly(procaterol hydrochloride) Uric acid Uric acid
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| GB/T 7714 | Kong, Dexian , Zhuang, Qizhao , Han, Yejian et al. Simultaneous voltammetry detection of dopamine and uric acid in human serum and urine with a poly(procaterol hydrochloride) modified glassy carbon electrode [J]. | TALANTA , 2018 , 185 : 203-212 . |
| MLA | Kong, Dexian et al. "Simultaneous voltammetry detection of dopamine and uric acid in human serum and urine with a poly(procaterol hydrochloride) modified glassy carbon electrode" . | TALANTA 185 (2018) : 203-212 . |
| APA | Kong, Dexian , Zhuang, Qizhao , Han, Yejian , Xu, Lanping , Wang, Zeming , Jiang, Lili et al. Simultaneous voltammetry detection of dopamine and uric acid in human serum and urine with a poly(procaterol hydrochloride) modified glassy carbon electrode . | TALANTA , 2018 , 185 , 203-212 . |
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Natural enzyme mimetics with high catalytic activity at nearly neutral pH values are highly desired for their applications in biological systems. Herein for the first time a stable MOF, namely MOF-808, has been shown to possess high intrinsic peroxidase-like catalytic activity under acidic, neutral, and alkaline conditions. As a novel peroxidase mimetic, MOF-808 can effectively catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine when H2O2 serves as oxidant, accompanied by a significant color variation in the solution. The catalytic activity and the color variation were greatly dependent on H2O2 concentration, and thus MOF-808 can be applied to the colorimetric sensing of H2O2. The H2O2 detection limit is 4.5 mu M, and the linear range is 10 mu M to 15 mM. In view of the significant inhibition effect produced by ascorbic acid, a facile and sensitive approach for colorimetric sensing of ascorbic acid was successfully established. The AA detection limit is 15 mu M, and the linear range is 30-1030 mu M. Further investigation found that the catalytic activity of MOF-808 could be mainly ascribed to the Zr-OH(OH2) groups. Such active Zr-OH(OH2) groups can be effectively shielded by gluconic acid, and subsequently the catalytic activity of MOF-808 was significantly suppressed. With these findings, a facile and selective colorimetric assay for glucose sensing has been successfully explored via combination of the glucose oxidation with the TMB oxidation. The glucose detection limit is 5.7 mu M, and the linear range is 5.7-1700 mu M. MOF-808 is one of the best colorimetric biosensors among the peroxidase mimics reported for H2O2, AA, and glucose detection.
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| GB/T 7714 | Zheng, He-Qi , Liu, Chun-Yan , Zeng, Xue-Yu et al. MOF-808: A Metal-Organic Framework with Intrinsic Peroxidase-Like Catalytic Activity at Neutral pH for Colorimetric Biosensing [J]. | INORGANIC CHEMISTRY , 2018 , 57 (15) : 9096-9104 . |
| MLA | Zheng, He-Qi et al. "MOF-808: A Metal-Organic Framework with Intrinsic Peroxidase-Like Catalytic Activity at Neutral pH for Colorimetric Biosensing" . | INORGANIC CHEMISTRY 57 . 15 (2018) : 9096-9104 . |
| APA | Zheng, He-Qi , Liu, Chun-Yan , Zeng, Xue-Yu , Chen, Jin , Lu, Jian , Lin, Rong-Guang et al. MOF-808: A Metal-Organic Framework with Intrinsic Peroxidase-Like Catalytic Activity at Neutral pH for Colorimetric Biosensing . | INORGANIC CHEMISTRY , 2018 , 57 (15) , 9096-9104 . |
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采用水热法制备了Ru-Mg Zr固体碱催化剂,通过XRD、XRF和CO2-TPD等方法对催化剂进行表征。结果显示,镁离子进入Zr O2晶格中形成固溶体,生成了新的碱性位点。试验结果表明,Ru-Mg Zr固体碱作为多相催化剂在亚油酸异构化反应中表现出良好的催化性能。在镁锆摩尔比为4∶1,催化剂用量为0.2 g,反应温度为180℃,反应时间为1.5 h的条件下,亚油酸的转化率达到65.09%。此外,催化剂重复使用5次后,没有明显的活性损失。通过气相色谱分析表明,产物主要为共轭亚油酸,其中以具有生物活性的9c,11t-CLA、10t,12c-CLA和9t,11t-CLA 3种异构体为主。
Keyword ：
亚油酸 亚油酸 共轭亚油酸 共轭亚油酸 固体碱催化剂 固体碱催化剂 多相催化剂 多相催化剂 异构化反应 异构化反应
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| GB/T 7714 | 陈新香 , 陈杰博 , 苏金为 et al. 固体碱催化剂Ru-MgZr催化制备共轭亚油酸的研究 [J]. | 中国粮油学报 , 2017 , 32 (09) : 99-103 . |
| MLA | 陈新香 et al. "固体碱催化剂Ru-MgZr催化制备共轭亚油酸的研究" . | 中国粮油学报 32 . 09 (2017) : 99-103 . |
| APA | 陈新香 , 陈杰博 , 苏金为 , 祁建民 . 固体碱催化剂Ru-MgZr催化制备共轭亚油酸的研究 . | 中国粮油学报 , 2017 , 32 (09) , 99-103 . |
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目的:构建特异抑制雌激素受体α(ERα)基因的短发夹RNA(sh RNA)慢病毒表达载体,检测其对ERα的干扰效果,并探讨其对MCF7细胞中乳腺癌干细胞含量的影响。方法:以人的ERα核酸序列为靶标,根据sh RNA设计原则设计并化学合成特异的sh RNA序列,经退火处理后与p SIH-H1质粒连接,转化大肠杆菌DH5α后挑取单克隆并测序;将该克隆进行病毒包装并感染乳腺癌MCF7细胞,用嘌呤霉素进行筛选,最终得到稳定克隆;收取细胞,用Western印迹和q RT-PCR检测ERα在m RNA及蛋白水平上的变化,流式细胞术检测敲低ERα能否影响MCF7乳腺癌干细胞的含量。结果:经测序分析表明目标sh RNA序列成功插入载体,q RT-PCR检测稳定克隆,发现该sh RNA能有效抑制目的基因的m RNA转录水平,同时Western印迹检测发现内源性ERα量明显下降;流式细胞术检测发现ERα敲低的MCF7稳定克隆中雌激素诱导的乳腺癌干细胞含量上升的现象被明显抑制。结论:设计并构建的抑制ERα的sh RNA能够有效抑制ERα的表达,并下调MCF7细胞中乳腺癌干细胞的含量,为研究ERα在乳腺癌干细胞中的功能及机制奠定了实验基础。
Keyword ：
流式细胞术 流式细胞术 短发夹RNA 短发夹RNA 肿瘤干细胞 肿瘤干细胞 雌激素受体α 雌激素受体α
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| GB/T 7714 | 杜佩云 , 程龙 , 姜丽娜 et al. 雌激素受体α短发夹RNA慢病毒载体的构建及其对乳腺癌干细胞的影响 [J]. | 生物技术通讯 , 2015 , 26 (03) : 325-328 . |
| MLA | 杜佩云 et al. "雌激素受体α短发夹RNA慢病毒载体的构建及其对乳腺癌干细胞的影响" . | 生物技术通讯 26 . 03 (2015) : 325-328 . |
| APA | 杜佩云 , 程龙 , 姜丽娜 , 陈立涵 , 林佳佳 , 叶棋浓 et al. 雌激素受体α短发夹RNA慢病毒载体的构建及其对乳腺癌干细胞的影响 . | 生物技术通讯 , 2015 , 26 (03) , 325-328 . |
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Sugarcane mosaic disease is caused by the Sugarcane streak mosaic virus (SCSMV; genus Poacevirus, family Potyviridae) which is common in some Asian countries. Here, we established a protocol of a one-step real-time quantitative reverse transcription PCR (real-time qRT-PCR) using the TaqMan probe for the detection of SCSMV in sugarcane. Primers and probes were designed within the conserved region of the SCSMV coat protein (CP) gene sequences. Standard single-stranded RNA (ssRNA) generated by PCR-based gene transcripts of recombinant pGEM-CP plasmid in vitro and total RNA extracted from SCSMV-infected sugarcane were used as templates of qRT-PCR. We further performed a sensitivity assay to show that the detection limit of the assay was 100 copies of ssRNA and 2 pg of total RNA with good reproducibility. The values obtained were approximately 100-fold more sensitive than those of the conventional RT-PCR. A higher incidence (68.6%) of SCSMV infection was detected by qRT-PCR than that (48.6%) with conventional RT-PCR in samples showing mosaic symptoms. SCSMV-free samples were verified by infection with Sugarcane mosaic virus (SCMV) or Sorghum mosaic virus (SrMV) or a combination of both. The developed qRT-PCR assay may become an alternative molecular tool for an economical, rapid, and efficient detection and quantification of SCSMV.
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| GB/T 7714 | Fu, Wei-Lin , Sun, Sheng-Ren , Fu, Hua-Ying et al. A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus [J]. | BIOMED RESEARCH INTERNATIONAL , 2015 , 2015 . |
| MLA | Fu, Wei-Lin et al. "A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus" . | BIOMED RESEARCH INTERNATIONAL 2015 (2015) . |
| APA | Fu, Wei-Lin , Sun, Sheng-Ren , Fu, Hua-Ying , Chen, Ru-Kai , Su, Jin-Wei , Gao, San-Ji . A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus . | BIOMED RESEARCH INTERNATIONAL , 2015 , 2015 . |
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目的:构建抑制TPP1基因的短发夹RNA(sh RNA)干扰载体。方法:以人的TPP1基因为靶序列,设计并合成sh RNA序列TPP1-si1和TPP1-si2,分别与RNA干扰慢病毒载体pll3.7连接,双酶切鉴定质粒得到阳性克隆pll-TPP1-si1和pll-TPP1-si2,经测序正确后将其与慢病毒包装载体(RRE、REV、VSVG)共转染293T细胞,进行病毒的包装,将得到的病毒感染稳定高表达外源TPP1蛋白的HT1080细胞,通过Western印迹检测其对TPP1蛋白表达的抑制效果,并进行比较;将抑制效果好的病毒感染高表达外源Pot1蛋白的Hep G2细胞,检测Pot1蛋白在内源TPP1被敲低的情况下能否在端粒定位。结果:经双酶切验证,外源片段成功插入载体pll-TPP1-si1和pll-TPP1-si2中;pll-TPP1-si1和pll-TPP1-si2均能明显抑制TPP1的表达,其中pll-TPP1-si1的抑制效果最好;pll-TPP1-si1病毒感染高表达外源Pot1蛋白的Hep G2细胞,经免疫荧光鉴定能够有效抑制内源TPP1的表达,使Pot1不再端粒定位。结论:构建的抑制TPP1基因的sh RNA干扰载体能有效抑制TPP1的表达,为端粒蛋白TPP1功能的研究奠定了实验基础。
Keyword ：
shRNA干扰 shRNA干扰 TPP1基因 TPP1基因 Western印迹 Western印迹 免疫荧光 免疫荧光
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| GB/T 7714 | 张金丁 , 金蕊 , 杨丹丹 et al. TPP1基因慢病毒干扰载体的构建及鉴定 [J]. | 生物技术通讯 , 2014 , 25 (06) : 760-764 . |
| MLA | 张金丁 et al. "TPP1基因慢病毒干扰载体的构建及鉴定" . | 生物技术通讯 25 . 06 (2014) : 760-764 . |
| APA | 张金丁 , 金蕊 , 杨丹丹 , 王亚楠 , 黄君健 , 苏金为 . TPP1基因慢病毒干扰载体的构建及鉴定 . | 生物技术通讯 , 2014 , 25 (06) , 760-764 . |
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本发明公开了一种促进荔枝树雌花分化的施药方法，在荔枝花两性期，采用吊液法对荔枝树滴注乙烯利溶液。乙烯是植物的内源激素，具有促进雌花分化，抑制雄花发育和控制树梢生长减少养分转移浪费，增加产量的作用。乙烯利为人工合成的化学调节剂，可被植物吸收在体内释放乙烯而发生功用。因此，本发明采用吊液法对荔枝树滴注乙烯利溶液，有效地调节了荔枝树的雌花分化，为荔枝的生产发展提供了重要的参考模式。
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| GB/T 7714 | 王湘平 , 苏金为 . 一种促进荔枝树雌花分化的施药方法 : CN201410058558.5[P]. | 2014-02-21 . |
| MLA | 王湘平 et al. "一种促进荔枝树雌花分化的施药方法" : CN201410058558.5. | 2014-02-21 . |
| APA | 王湘平 , 苏金为 . 一种促进荔枝树雌花分化的施药方法 : CN201410058558.5. | 2014-02-21 . |
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本发明公开了一种锡酸盐催化制备共轭亚油酸的方法，选用亚油酸，直接利用锡酸盐催化异构化、过滤催化剂得到共轭亚油酸。本发明的催化剂具有催化效率高，催化剂价格便宜，稳定性能良好，后处理简单，生产成本低，生产过程不产生污染等特点。
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| GB/T 7714 | 陈杰博 , 陈新香 , 蔡珠玉 et al. 一种锡酸盐催化制备共轭亚油酸的方法 : CN201310207552.5[P]. | 2013-05-30 . |
| MLA | 陈杰博 et al. "一种锡酸盐催化制备共轭亚油酸的方法" : CN201310207552.5. | 2013-05-30 . |
| APA | 陈杰博 , 陈新香 , 蔡珠玉 , 苏金为 . 一种锡酸盐催化制备共轭亚油酸的方法 : CN201310207552.5. | 2013-05-30 . |
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