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Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits SCIE
期刊论文 | 2025 , 47 (2) | CURRENT ISSUES IN MOLECULAR BIOLOGY
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Tea oil is an important high-quality edible oil derived from woody plants. Camellia oleifera is the largest and most widely planted oil-producing plant in the Camellia genus in China, and its seeds are the most important source for obtaining tea oil. In current research, improving the yield and quality of tea oil is the main goal of oil tea genetic breeding. The aim of this study was to investigate the degree of genetic variation in an early crossing population of C. oleifera and identify single nucleotide polymorphisms (SNPs) and genes significantly associated with fruit traits, which can provide a basis for marker-assisted selection and gene editing for achieving trait improvement in the future. In this study, we selected a crossing population of approximately 40-year-old C. oleifera with a total of 330 samples. Then, ddRAD sequencing was used for SNP calling and population genetic analysis, and association analysis was performed on fruit traits measured repeatedly for two consecutive years. The research results indicate that over 8 million high-quality SNPs have been identified, but the vast majority of SNPs occur in intergenic regions. The nucleotide polymorphism of this population is at a low level, and Tajima's D values are mostly greater than 0, indicating that the change in this population was not suitable for the model of central evolution. The population structure analysis shows that the population has seven theoretical sources of genetic material and can be divided into seven groups, and the clustering analysis results support the population structure analysis results. Association analysis identified significant SNPs associated with genes related to the seed number of a single fruit and seed kernel oil content. Our findings provide a basis for molecular breeding and future genetic improvement of cultivated oil tea.

Keyword :

association analysis association analysis Camellia oleifera Camellia oleifera crossing population crossing population genetic variation genetic variation SNP SNP

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GB/T 7714 Zhou, Lexin , Li, Yu , Ye, Ling et al. Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (2) .
MLA Zhou, Lexin et al. "Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 47 . 2 (2025) .
APA Zhou, Lexin , Li, Yu , Ye, Ling , Li, Jiani , Liang, Tian , Liu, Yanxuan et al. Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (2) .
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锥栗农家品种‘油榛’栽培群体的SSR变异分析
期刊论文 | 2024 , 22 (05) , 1508-1513 | 分子植物育种
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‘油榛’是福建省建瓯市广泛种植的优良农家品种之一。本研究利用SSR分子标记对‘油榛’栽培群体进行遗传多样性分析,探索其变异情况,结果表明:由10个SSR引物组合对锥栗农家品种‘油榛’栽培群体的30个植株进行PCR扩增,共计扩增出26个条带,多态性条带26个,多态性条带占比100%,30个植株的遗传相似性系数在0.115 4~1.000 0之间,平均为0.877 2,表明锥栗农家品种‘油榛’具有较高的遗传多样性。为了解‘油榛’栽培群体30个植株的亲缘关系,对30个植株进行聚类分析,其中YZ5、YZ1单独各聚为一类,其余28个植株聚为一类。研究结果为在锥栗农家品种栽培群体中进行优树选择提供了理论依据。

Keyword :

SSR SSR 农家品种 农家品种 油榛 油榛 遗传多样性 遗传多样性 锥栗 锥栗

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GB/T 7714 叶树涛 , 方周 , 李颖林 et al. 锥栗农家品种‘油榛’栽培群体的SSR变异分析 [J]. | 分子植物育种 , 2024 , 22 (05) : 1508-1513 .
MLA 叶树涛 et al. "锥栗农家品种‘油榛’栽培群体的SSR变异分析" . | 分子植物育种 22 . 05 (2024) : 1508-1513 .
APA 叶树涛 , 方周 , 李颖林 , 吴钧剑 , 郑国华 , 陈辉 et al. 锥栗农家品种‘油榛’栽培群体的SSR变异分析 . | 分子植物育种 , 2024 , 22 (05) , 1508-1513 .
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福建省主栽普通油茶优良无性系遗传差异的SRAP分析
期刊论文 | 2024 , 22 (17) , 5663-5669 | 分子植物育种
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油茶是中国重要的木本油料树种,具有较高的利用价值。本研究利用SRAP分子标记对10个福建省主栽普通油茶优良无性系进行分析,探讨其遗传差异,结果表明:从256对SRAP引物组合中筛选出12对引物对10个优良无性系进行PCR扩增,共获得186个条带,其中多态性条带166个,多态性条带的比率为89.2%。EM-2/ME-4引物组合扩增的条带多态性最高,为100%;多态性信息量最小值和最大值分别为0.280和0.394,多态性信息量平均值为0.330。10个优良无性系遗传相似系数最小值和最大值分别为0.591 4和0.774 2,遗传相似系数平均值为0.670 8。聚类分析表明,遗传相似系数为0.677 5处,10个普通油茶优良无性系可以划为4大类:闽43和闽46聚为第Ⅰ类,闽48、闽49、闽53聚为第Ⅱ类,闽50聚为第Ⅲ类,闽54、闽56、闽60、闽62聚为第Ⅳ类。这10个优良无性系之间存在着较高的遗传多样性水平,研究结果为普通油茶杂交育种和分子育种工作的开展提供参考。

Keyword :

SRAP SRAP 无性系 无性系 普通油茶 普通油茶 遗传差异 遗传差异

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GB/T 7714 王庭雄 , 李祥胜 , 梁诗强 et al. 福建省主栽普通油茶优良无性系遗传差异的SRAP分析 [J]. | 分子植物育种 , 2024 , 22 (17) : 5663-5669 .
MLA 王庭雄 et al. "福建省主栽普通油茶优良无性系遗传差异的SRAP分析" . | 分子植物育种 22 . 17 (2024) : 5663-5669 .
APA 王庭雄 , 李祥胜 , 梁诗强 , 叶树涛 , 吕欢欢 , 陈辉 et al. 福建省主栽普通油茶优良无性系遗传差异的SRAP分析 . | 分子植物育种 , 2024 , 22 (17) , 5663-5669 .
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锥栗半同胞家系栗疫病抗性与生长性状的遗传分析
期刊论文 | 2024 , 44 (02) , 175-182 | 森林与环境学报
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为筛选出生长良好且栗疫病抗性强的优良家系和优良单株,对12个锥栗半同胞家系的栗疫病抗性相关性状及生长性状进行变异分析、方差分析、相关性分析和遗传参数估算。结果表明,12个锥栗半同胞家系各性状的平均变异系数为20.19%,病斑面积的变异系数最大,为39.76%,叶形指数的变异系数最小,为11.14%。病斑宽、叶面积、叶周长、叶长、叶脉数在家系间具有显著差异(P<0.05),感病率、感病指数、叶宽在家系间具有极显著差异(P<0.01)。苗高与感病率呈显著负相关(P<0.05),与感病指数呈极显著负相关(P<0.01);叶长与感病率、感病指数呈极显著负相关(P<0.01)。筛选出JO14(‘白露仔’)、JO8(‘油桐仔’)、JO2(‘大苞榛’) 3个优良家系;筛选出9株(JO2-25、JO13-29、JO14-32、JO2-12、JO8-17、JO3-4、JO5-26、JO2-19、JO12-20)栗疫病抗性强的优良单株,4株(JO2-5、JO7-16、JO8-17、JO14-3)生长良好的优良单株,其中JO8-17抗性强且生长良好。

Keyword :

优良单株 优良单株 优良家系 优良家系 半同胞家系 半同胞家系 栗疫病抗性 栗疫病抗性 锥栗 锥栗

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GB/T 7714 吴美珍 , 李佳妮 , 李煜 et al. 锥栗半同胞家系栗疫病抗性与生长性状的遗传分析 [J]. | 森林与环境学报 , 2024 , 44 (02) : 175-182 .
MLA 吴美珍 et al. "锥栗半同胞家系栗疫病抗性与生长性状的遗传分析" . | 森林与环境学报 44 . 02 (2024) : 175-182 .
APA 吴美珍 , 李佳妮 , 李煜 , 吴钧剑 , 杨金辉 , 陈辉 . 锥栗半同胞家系栗疫病抗性与生长性状的遗传分析 . | 森林与环境学报 , 2024 , 44 (02) , 175-182 .
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浙江红花油茶实生群体性状变异及综合评价
期刊论文 | 2024 , 44 (03) , 274-282 | 森林与环境学报
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为研究浙江红花油茶实生群体的表型变异规律及优良单株筛选方法,加强对浙江红花油茶基因资源的保护和育种利用,以浙江红花油茶(Camellia chekiangoleosa)实生群体为研究对象,对其表型性状进行变异分析、相关性分析、回归分析和通径分析,结合主成分分析、模糊隶属函数法和多维空间坐标综合评定值累加法进行优良单株的选择。结果表明:单株之间变异丰富,25个表型性状的变异系数为5.74%~57.16%,多样性指数为1.934 6~2.095 9,产量经济性状的变异幅度最大,多样性指数最小;回归分析与通径分析确定了果实数、单果鲜籽重、单果干仁重和种仁含油率是影响单株产油量的关键表型性状,其中,单果干仁重是主要决策变量,果实数是主要限制变量;主成分分析将25个性状转化为6个主成分因子,累计贡献率为86.557%。根据以上研究结果筛选出9株优良单株,可作为浙江红花油茶丰产优质新品种的选育材料。

Keyword :

主成分分析 主成分分析 优良单株 优良单株 实生群体 实生群体 浙江红花油茶 浙江红花油茶 表型性状 表型性状 通径分析 通径分析

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GB/T 7714 李佳妮 , 吴美珍 , 李煜 et al. 浙江红花油茶实生群体性状变异及综合评价 [J]. | 森林与环境学报 , 2024 , 44 (03) : 274-282 .
MLA 李佳妮 et al. "浙江红花油茶实生群体性状变异及综合评价" . | 森林与环境学报 44 . 03 (2024) : 274-282 .
APA 李佳妮 , 吴美珍 , 李煜 , 叶玲 , 陈世品 , 陈辉 . 浙江红花油茶实生群体性状变异及综合评价 . | 森林与环境学报 , 2024 , 44 (03) , 274-282 .
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锥栗农家品种人工授粉杂交果实性状的变异分析
期刊论文 | 2024 , 53 (01) , 1-6 | 福建农林大学学报(自然科学版)
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【目的】筛选锥栗优良授粉组合与杂交组合。【方法】以5个锥栗主栽优良农家品种为试验材料进行杂交授粉,对所得果实性状进行变异分析。【结果】16个杂交组合果实性状间变异幅度最大的为栗苞质量、坚果质量,变异幅度最小为果型指数,不同组合间性状差异显著,具有较好的选择潜力;总苞质量、坚果质量、坚果长、坚果宽、出籽率等性状间具有不同程度的正相关性,可进行联合选择;总苞质量、坚果质量、栗苞长、栗苞宽、栗苞高、出籽率等性状存在不同程度的花粉直感效应;以亲本ZL1为母本、亲本ZL12为父本的各授粉组合果实性状表现优良,并选择出5个优良授粉组合;ZL1的GCA效应较高,而杂交组合ZL1×ZL9、ZL6×ZL12、ZL3×ZL6的SCA效应最高。【结论】研究结果可为锥栗杂交育种和丰产栽培提供参考。

Keyword :

人工授粉 人工授粉 农家品种 农家品种 变异分析 变异分析 杂交育种 杂交育种 锥栗 锥栗

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GB/T 7714 方周 , 顾光仕 , 李煜 et al. 锥栗农家品种人工授粉杂交果实性状的变异分析 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (01) : 1-6 .
MLA 方周 et al. "锥栗农家品种人工授粉杂交果实性状的变异分析" . | 福建农林大学学报(自然科学版) 53 . 01 (2024) : 1-6 .
APA 方周 , 顾光仕 , 李煜 , 杨金辉 , 江锡兵 , 陈辉 . 锥栗农家品种人工授粉杂交果实性状的变异分析 . | 福建农林大学学报(自然科学版) , 2024 , 53 (01) , 1-6 .
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锥栗优良无性系遗传多样性的EST-SSR分析
期刊论文 | 2024 , 44 (05) , 484-491 | 森林与环境学报
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为促进锥栗新品种的选育、种质资源的鉴定和锥栗产业的健康发展,以福建省建瓯市栽培的22个锥栗优良无性系为研究对象,利用开发的6对多态性表达序列标签-简单重复序列(EST-SSR)引物对其遗传多样性进行研究,并构建指纹图谱。结果表明,22个锥栗无性系平均每对引物的等位基因数为2.667,平均有效等位基因数为2.038,平均观测杂合度为0.349,平均期望杂合度为0.447,平均Shannon信息指数为0.721,平均多态性位点数为11.500,平均多态性信息含量为0.434,表明锥栗无性系遗传多样性较高。遗传相似系数变化范围为0.754~1.000,平均值为0.856;在遗传相似性系数为0.860处,可将其划分为5个大类:第Ⅰ类仅包括HL19;第Ⅱ类包括HL5、 HL6、 HL8、 HL21;第Ⅲ类包括HL17、 HL18;第Ⅳ类包括HL12、 HL13;第Ⅴ类包括HL1、 HL2、 HL3、 HL4、 HL7、 HL9、 HL10、 HL11、 HL14、 HL15、 HL16、 HL20、 HL22。利用3对多态性引物,共构建了22个锥栗无性系的指纹图谱。

Keyword :

指纹图谱 指纹图谱 无性系 无性系 表达序列标签-简单重复序列 表达序列标签-简单重复序列 遗传多样性 遗传多样性 锥栗 锥栗

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GB/T 7714 杨金辉 , 王一帆 , 李颖林 et al. 锥栗优良无性系遗传多样性的EST-SSR分析 [J]. | 森林与环境学报 , 2024 , 44 (05) : 484-491 .
MLA 杨金辉 et al. "锥栗优良无性系遗传多样性的EST-SSR分析" . | 森林与环境学报 44 . 05 (2024) : 484-491 .
APA 杨金辉 , 王一帆 , 李颖林 , 江锡兵 , 陈辉 , 李煜 . 锥栗优良无性系遗传多样性的EST-SSR分析 . | 森林与环境学报 , 2024 , 44 (05) , 484-491 .
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Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera SCIE
期刊论文 | 2024 , 14 (12) | AGRONOMY-BASEL
WoS CC Cited Count: 1
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Advancement of the oil tea industry requires the development of high-yielding and superior-quality varieties of Camellia oleifera, a major oilseed crop. However, traditional breeding methods, hampered by lengthy cycles and low selection accuracy, significantly constrain the breeding process. Identifying single nucleotide polymorphisms (SNPs) associated with target traits, and applying molecular marker-assisted selection (MAS) for these traits, can thereby shorten the breeding cycles and amplify the breeding efficiency. In this study, we utilized the hexaploid C. oleifera as the reference genome to identify high-quality SNPs and constructed a high-density genetic linkage map of C. oleifera that spanned 1566.733 cM, included 3097 SNPs, and was anchored to 15 linkage groups. Using interval mapping, we localized quantitative trait loci (QTLs) for 11 juvenile traits in C. oleifera, identifying 15 QTLs for growth traits and 24 QTLs for leaf traits, including 4 stable QTLs. The logarithm of odds (LOD) scores for individual QTLs ranged from 3.48 to 14.62, explaining 9.86-48.61% of the phenotypic variance. We further identified 2 SNPs associated with growth traits (marker11-951 and marker12-68) and 10 SNPs associated with leaf traits (marker11-276, marker11-410, marker11-560, marker13-16, marker13-39, marker13-110, marker13-731, marker14-701, marker14-910, and marker14-1331). These results provide valuable insights into the genetic mapping of key traits in C. oleifera and will contribute to the development of new varieties with high yield and superior quality in the future.

Keyword :

Camellia oleifera Camellia oleifera genetic linkage map genetic linkage map juvenile traits juvenile traits QTL QTL SNP SNP

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GB/T 7714 Ye, Ling , Li, Yu , Liu, Yanxuan et al. Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera [J]. | AGRONOMY-BASEL , 2024 , 14 (12) .
MLA Ye, Ling et al. "Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera" . | AGRONOMY-BASEL 14 . 12 (2024) .
APA Ye, Ling , Li, Yu , Liu, Yanxuan , Zhou, Lexin , Li, Jia'ni , Liang, Tian et al. Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera . | AGRONOMY-BASEL , 2024 , 14 (12) .
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Landscape Fragmentation and Spatial Autocorrelation of a Typical Watershed in the Wenchuan Earthquake-Affected Area-A Case Study in the Longxi River Basin SCIE
期刊论文 | 2023 , 14 (12) | FORESTS
WoS CC Cited Count: 3
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The 2008 8.0 Ms Wenchuan earthquake caused serious ecological degradation in the northwest of China's Sichuan Province. Elucidating landscape fragmentation and spatial pattern of post-earthquake landscape is of great interest in order to improve ecological restoration and predict the spatial distribution of damaged ecosystems in earthquake-affected areas. We used four real-time remote sensing images to analyze the landscape pattern characteristics and spatial autocorrelation of the Longxi River Basin before and after the Wenchuan earthquake during the period from 2005 to 2015. In the study period, the degree of landscape fragmentation increased 1 year after the earthquake in 2009 but decreased in 2011 and 2015. The spatial distribution of forest, farmland, and shrub-grassland had significantly positive spatial correlation and the characteristics of spatial aggregation from 2005 to 2015. Construction land had no significant spatial correlation before the earthquake in 2005, but significantly positive spatial correlation after the earthquake, while traffic land had no significant spatial correlation across the study period. Unlike the other landscape types, geological disasters changed from a significantly negative spatial correlation before the earthquake to a significantly positive spatial correlation after the earthquake. However, the positive autocorrelation of all landscape types decreased with the increase of space distance, but in different distance-decay rates. The High-high spatial aggregation areas of geological disasters and construction land clustered gradually upstream of the basin and distributed in Longchi town, respectively; those of farmland distributed in the southeast of the basin increased in 2009 but then decreased, while those of forests had the opposite pattern as the dominant landscape type after the earthquake. Our results elucidated the spatial structure and distribution features of the Longxi River Basin to give a theoretical foundation for assessing the effects of ecological restoration and reconstruction management in earthquake-affected areas.

Keyword :

landscape fragmentation landscape fragmentation landscape pattern landscape pattern spatial autocorrelation spatial autocorrelation typical watershed typical watershed Wenchuan earthquake Wenchuan earthquake

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GB/T 7714 Tian, Xue , Yang, Liusheng , Wu, Xuan et al. Landscape Fragmentation and Spatial Autocorrelation of a Typical Watershed in the Wenchuan Earthquake-Affected Area-A Case Study in the Longxi River Basin [J]. | FORESTS , 2023 , 14 (12) .
MLA Tian, Xue et al. "Landscape Fragmentation and Spatial Autocorrelation of a Typical Watershed in the Wenchuan Earthquake-Affected Area-A Case Study in the Longxi River Basin" . | FORESTS 14 . 12 (2023) .
APA Tian, Xue , Yang, Liusheng , Wu, Xuan , Wu, Jianzhao , Guo, Yiting , Guo, Yuhao et al. Landscape Fragmentation and Spatial Autocorrelation of a Typical Watershed in the Wenchuan Earthquake-Affected Area-A Case Study in the Longxi River Basin . | FORESTS , 2023 , 14 (12) .
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汶川地震灾区典型小流域景观格局的演变特征——以龙溪河流域为例
期刊论文 | 2023 , 29 (02) , 449-459 | 应用与环境生物学报
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为探讨地震前后典型小流域的景观格局变化特征,以龙溪河流域为研究区,选取2005年、2009年、2011年、2015年四期高分辨率遥感影像为数据源.从斑块类型和景观两个水平,运用13种景观格局指数,通过Fragstats景观指数计算软件,对龙溪河小流域震前(2005)、震后初期(2009)、震后3年(2011)和震后7年(2015)等4个不同时期景观格局进行测度,并利用ArcGIS软件建立缓冲区,将4个时段的遥感影像划分为人类活动区域和自然区域,研究该流域在地震前后的景观生态格局演变特征.结果表明:(1)2005年-2015年受地震灾害影响各斑块面积均发生不同程度的改变,其中,林地景观斑块面积占比均最大,震前91.35%降至78.41%,震后3年增加至86.06%,震后7年继续增加至90.46%,构成龙溪河流域景观基质.林地斑块面积地震前后差异较小;耕地、建设用地景观斑块面积呈持续减小趋势;灌草景观斑块面积呈先减后增再减的波动减小趋势;交通用地呈持续增加趋势;沟道、裸地、地质灾害斑块面积呈先增后减的趋势.(2)2005-2015年,都江堰龙溪河流域的景观类型变化以灌草、地质灾害和林地的空间转换为主.2005-2009年景观面积转移特征为:灌草转变为林地,林地转变为地质灾害;2009年-2011年转移特征为:灌草转变为林地,林地转变为地质灾害;2011年-2015年转移特征为:灌草、地质灾害转变为林地,林地转变为灌草、地质灾害;2005年-2015年整体转移特征为:灌草转变为林地,林地转变为地质灾害、灌草.(3)震后林地、灌草、交通用地、裸地、地质灾害的景观破碎程度均较高,受到较强的人为干扰.林地与地质灾害受地震影响最为显著,边界被割裂的程度较深,景观破碎度高,边界效应较明显,但林地作为景观基质聚合度较其余景观斑块高.自然区域中,人为干扰相对较小,震后地质灾害的斑块密度与边缘密度均大幅减少,且低于人类活动区,这表明地震及次生灾害对自然区域的影响较小,且无人类干扰时可以较好地恢复.(4)震后SHEI与SHDI呈增长趋势,CONTAG呈减小趋势,表明龙溪河流域各个景观类型面积差异性减小,斑块类型增加,景观类型的优势度降低,景观水平异质性增强,景观连通性降低.自然区域景观分布较人类活动区域分布聚集且邻接性高,人类活动区域景观的分布受制于地形因子的作用.研究发现“5·12”大地震破坏了龙溪河流域的原有景观格局,泥石流、滑坡等次生灾害破坏其中原有斑块,灾后人类活动与景观格局的变化联系密切.本研究可为龙溪河流域的生境恢复与灾后重建工作提供依据,并为其生态保护和可持续发展提供理论基础和科学建议.(图5表8参43)

Keyword :

地质灾害 地质灾害 景观指数 景观指数 景观破碎化 景观破碎化 景观空间格局 景观空间格局 龙溪河 龙溪河

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GB/T 7714 田雪 , 吴建召 , 杨鸿飞 et al. 汶川地震灾区典型小流域景观格局的演变特征——以龙溪河流域为例 [J]. | 应用与环境生物学报 , 2023 , 29 (02) : 449-459 .
MLA 田雪 et al. "汶川地震灾区典型小流域景观格局的演变特征——以龙溪河流域为例" . | 应用与环境生物学报 29 . 02 (2023) : 449-459 .
APA 田雪 , 吴建召 , 杨鸿飞 , 俞陈辉 , 陈辉 , 林勇明 et al. 汶川地震灾区典型小流域景观格局的演变特征——以龙溪河流域为例 . | 应用与环境生物学报 , 2023 , 29 (02) , 449-459 .
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