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学者姓名：潘东明
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Abstract ：
果实大小是果实的重要经济性状之一，直接影响作物产量、品质以及商品性。果实的生长发育过程既受到作物种类、品种固有的遗传背景的影响，也受到环境因素和栽培管理因素等方面的影响，而前者的影响是更为直接和深刻。综述了近年来与园艺植物果实大小调控相关的功能基因和转录因子的研究概况，着重介绍了番茄、黄瓜、梨、葡萄、苹果和柑橘等园艺作物中POS1、FW、YABBY、WOX、OFP、NCED、YUC、GAox20、AREB等基因的研究进展，为今后研究果实大小调控提供理论参考。
Keyword ：
大小 大小 果实 果实 相关基因 相关基因 调控 调控
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| GB/T 7714 | 李杰 , 罗奕 , 张琪悦 et al. 调控果实大小相关基因的研究进展 [J]. | 福建农业科技 , 2023 , 54 (05) : 28-36 . |
| MLA | 李杰 et al. "调控果实大小相关基因的研究进展" . | 福建农业科技 54 . 05 (2023) : 28-36 . |
| APA | 李杰 , 罗奕 , 张琪悦 , 潘腾飞 , 于远 , 佘文琴 et al. 调控果实大小相关基因的研究进展 . | 福建农业科技 , 2023 , 54 (05) , 28-36 . |
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Overaccumulation of lignin (a physiological disorder known as granulation) often occurs during fruit ripening and postharvest storage in pomelo (Citrus grandis). It causes an unpleasant fruit texture and taste. Previous studies have shown that lignin metabolism is closely associated with the process of juice sacs granulation. At present, the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate NAC transcription factor, CgNAC043, that is involved in the regulation of lignin biosynthesis in Citrus grandis, which has homologs in Arabidopsis and other plants. We used the fruit juice sacs of 'San hong' as the material, the staining for lignin with HCl-phloroglucinol of fruit juice sacs became dark red from the various developmental stages at 172 to 212 days post anthesis (DPA). The RT-qPCR was used to analyze the gene expression of CgNAC043 and its target gene CgMYB46 in fruit sacs, it was found that the expression trend of CgNAC043 was basically same as CgMYB46, which increased gradually and peaked at 212 DPA. The expression level of CgNAC043 in juice sacs obtained away from the core was the lowest, while those near the core and granulated area were highly expressed. The transcriptional activation activity of CgNAC043 and CgMYB46 was analyzed by a yeast two-hybrid system, with only CgNAC043 showing transcriptional activation activity in Y2H Gold yeast. A transformation vector, p1301- CgNAC043, was transformed into the mesocarp of 'San hong' by Agrobacterium-mediated transformation. Results showed that the expression of transcription factors CgMYB58 and CgMYB46 are all upregulated. Further experiments proved that CgNAC043 not only can directly trans-activate the promoter of CgMYB46 but also trans-activate the promoters for the lignin biosynthesis-related genes CgCCoAOMT and CgC3H by dual luciferase assay. We isolated the CgNAC043 gene in pomelo and found CgNAC043 regulates target genes conferring the regulation of juice sacs granulation.
Keyword ：
dual luciferase dual luciferase gene expression gene expression MYB MYB NAC NAC pomelo pomelo transcriptional activity transcriptional activity
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| GB/T 7714 | Li, Xiaoting , Wang, Naiyu , She, Wenqin et al. Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis "San Hong" [J]. | PLANTS-BASEL , 2022 , 11 (3) . |
| MLA | Li, Xiaoting et al. "Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis "San Hong"" . | PLANTS-BASEL 11 . 3 (2022) . |
| APA | Li, Xiaoting , Wang, Naiyu , She, Wenqin , Guo, Zhixiong , Pan, Heli , Yu, Yuan et al. Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis "San Hong" . | PLANTS-BASEL , 2022 , 11 (3) . |
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Juice sac granulation (a physiological disorder) leads to large postharvest losses of pomelo (Citrus maxima). Previous studies have shown that juice sac granulation is closely related to lignin accumulation, while the molecular mechanisms underlying this disorder remain elusive in pomelo. Our results showed that the lignin content in NC (near the core) and FC (far away from the core) juice sacs overall increased from 157 DPA (days post anthesis) to 212 DPA and reached a maximum at 212 DPA. Additionally, the lignin content of NC juice sacs was higher than that of FC juice sacs. In this study, we used transcriptome-based weighted gene co-expression network analysis (WGCNA) to address how lignin formation in NC and FC juice sacs is generated during the development of pomelo. After data assembly and bioinformatic analysis, we found a most correlated module (black module) to the lignin content, then we used the 11 DEGs in this module as hub genes for lignin biosynthesis. Among these DEGs, PAL (phenylalanine ammonia lyase), HCT (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase), 4CL2 (4-coumarate: CoA ligase), C4H (cinnamate 4-hydroxylase), C3 ' H (p-coumarate 3-hydroxylase), and CCoAOMT1 (caffeoyl CoA 3-Omethyltransferase) were the most distinct DEGs in granulated juice sacs. Co-expression analysis revealed that the expression patterns of several transcription factors such as MYB, NAC, OFP6, and bHLH130 are highly correlated with lignin formation. In addition, the expression patterns of the DEGs related to lignin biosynthesis and transcription factors were validated by qRT-PCR, and the results were highly concordant with the RNA-seq results. These results would be beneficial for further studies on the molecular mechanism of lignin accumulation in pomelo juice sacs and would help with citrus breeding.
Keyword ：
granulation granulation lignin lignin pomelo pomelo transcriptome transcriptome WGCNA WGCNA
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| GB/T 7714 | Li, Xiaoting , Huang, Hantang , Rizwan, Hafiz Muhammad et al. Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima) [J]. | GENES , 2022 , 13 (5) . |
| MLA | Li, Xiaoting et al. "Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima)" . | GENES 13 . 5 (2022) . |
| APA | Li, Xiaoting , Huang, Hantang , Rizwan, Hafiz Muhammad , Wang, Naiyu , Jiang, Jingyi , She, Wenqin et al. Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima) . | GENES , 2022 , 13 (5) . |
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为了探讨荔枝果皮和假种皮(果肉)可溶性糖、有机酸组分在果实不同发育阶段的积累和代谢转化,本研究以‘乌叶’和‘兰竹’荔枝为试材,通过UPLC建立果实糖酸组分的定量方法,并对其变化进行分析比较。结果表明,成熟果实果皮及假种皮的可溶性糖含量较发育期显著上升,果皮以果糖积累为主,而假种皮则以蔗糖积累为主。发育期果实果皮富含莽草酸和奎尼酸,两者合计占比高达90%,至果实成熟仍占比75%;果皮中其余酸组分较发育期均有所增加。假种皮有机酸组分以苹果酸为主,但伴随果实成熟,其含量呈明显的下降趋势。研究首次利用UPLC分析荔枝果实糖酸组分的变化,表明在不同发育阶段果皮与假种皮中的糖酸组分存在不同的消长。
Keyword ：
UPLC UPLC 假种皮 假种皮 可溶性糖 可溶性糖 有机酸 有机酸 果皮 果皮 荔枝 荔枝
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| GB/T 7714 | 蔡灿军 , 郭志雄 , 潘腾飞 et al. 应用UPLC分析荔枝果皮、假种皮可溶性糖和有机酸组分的变化 [J]. | 中国农学通报 , 2021 , 37 (31) : 139-144 . |
| MLA | 蔡灿军 et al. "应用UPLC分析荔枝果皮、假种皮可溶性糖和有机酸组分的变化" . | 中国农学通报 37 . 31 (2021) : 139-144 . |
| APA | 蔡灿军 , 郭志雄 , 潘腾飞 , 尤有利 , 郭雅倩 , 李瑞 et al. 应用UPLC分析荔枝果皮、假种皮可溶性糖和有机酸组分的变化 . | 中国农学通报 , 2021 , 37 (31) , 139-144 . |
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Abstract ：
【目的】研究探明琯溪蜜柚及其芽变新品种果汁的香气组分种类、相对含量与差异,以及引起香气组分变化的可能分子机理。【方法】以琯溪蜜柚及其芽变品种红肉蜜柚和三红蜜柚果实为试材,采用气相色谱质谱法(GC-MS)测定果汁香气组分;通过转录组分析,筛选差异表达显著的基因,并采用FPKM值的趋势分析这些基因的表达模式。【结果】供试3个果汁样品中共检出150种香气组分,占组分数量较多的香气化合物为芳香烃类、倍半萜烯类、醛类、醇类、酮类等;各品种的香气组分数量与含量相差较大,琯溪蜜柚、红肉蜜柚和三红蜜柚分别检出59种、129种和67种香气化合物,其总相对含量分别为4.56、8.02和9.90,说明芽变显著引起了香气组分和总相对含量的变化。香气组分中醛类相对含量最高,己醛为3个品种的共同主要香气组分;醇类、酮类相对含量次之,呋喃类、芳香烃类、倍半萜烯类、酯类、单萜类化合物相对含量较少;此外还检出相对含量很低或微量的其他类的香气组分32种,且均为未见报道的未知化合物。利用转录组分析筛选获得新基因(Citrus_maxima_newGene_12651)1个,表达差异显著的脂肪氧化酶(Lipoxidase)LOX2.1基因7个(cg2g001970、cg2g001980、cg2g002000、cg2g002010、 cg2g002030、 cg2g002040、 cg2g002080)、 LOX3.1基因1个(cg1g010660)和乙醇脱氢酶(Alcohol dehydrogenase)ADH1基因2个(cg3g017900、cg3g017890),采用FPKM值的趋势分析,初步推测3个品种的果汁香气组分差异与脂肪酸途径、异戊二烯途径中的基因差异表达有关。【结论】醛类、醇类、酮类是琯溪蜜柚及其芽变品种红肉蜜柚和三红蜜柚的主要香气组分,己醛为3个品种的共同主要香气化合物;供试3个品种果汁香气组分差异显著,初步推测与脂肪酸途径、异戊二烯途径等途径中的基因差异表达有关。
Keyword ：
差异表达基因 差异表达基因 柚子 柚子 琯溪蜜柚 琯溪蜜柚 芽变品种 芽变品种 香气组分 香气组分
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| GB/T 7714 | 潘鹤立 , 张鑫鑫 , 潘腾飞 et al. 琯溪蜜柚及其芽变品种红肉蜜柚、三红蜜柚果汁香气组分研究 [J]. | 福建农业学报 , 2021 , 36 (03) : 279-289 . |
| MLA | 潘鹤立 et al. "琯溪蜜柚及其芽变品种红肉蜜柚、三红蜜柚果汁香气组分研究" . | 福建农业学报 36 . 03 (2021) : 279-289 . |
| APA | 潘鹤立 , 张鑫鑫 , 潘腾飞 , 潘东明 , 于远 . 琯溪蜜柚及其芽变品种红肉蜜柚、三红蜜柚果汁香气组分研究 . | 福建农业学报 , 2021 , 36 (03) , 279-289 . |
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‘六月早’蜜柚(Citrus maxima ‘Liuyuezao’)为国内重要的柚品种琯溪蜜柚早熟芽变品种。以‘六月早’蜜柚为材料,利用二代测序进行全基因组重测序,从中组装获取叶绿体基因组并对其进行注释。结果表明:组装获得的‘六月早’蜜柚的叶绿体基因组全长160 186 bp,四分体结构由大单拷贝区(large single copy, LSC)、小单拷贝区(small single copy, SSC)和反向重复区(inverted repeat, IR)组成,3个分区的长度分别为87 939、18 395、26 926 bp。注释得到133个基因,其中包含89个编码基因,37个tRNA和8个rRNA。共识别到31个短串联重复序列,101个SSR位点。将已公开发表的29个芸香科叶绿体基因组使用最大似然法进行系统发育关系的构建,结果表明,‘六月早’蜜柚与甜橙(C. sinensis)、柠檬(C. limon)和C. platymamma的亲缘关系较近。
Keyword ：
SSR SSR 叶绿体基因组 叶绿体基因组 琯溪蜜柚 琯溪蜜柚 系统发育 系统发育
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| GB/T 7714 | 徐世荣 , 陈燕琼 , 潘东明 et al. ‘六月早’蜜柚叶绿体基因组及其特征分析 [J]. | 热带作物学报 , 2021 , 42 (05) : 1223-1230 . |
| MLA | 徐世荣 et al. "‘六月早’蜜柚叶绿体基因组及其特征分析" . | 热带作物学报 42 . 05 (2021) : 1223-1230 . |
| APA | 徐世荣 , 陈燕琼 , 潘东明 , 潘鹤立 . ‘六月早’蜜柚叶绿体基因组及其特征分析 . | 热带作物学报 , 2021 , 42 (05) , 1223-1230 . |
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为探讨乙烯在水仙鳞茎膨大过程中的作用以及种球对乙烯信号的响应,本试验以多花水仙种球膨大期的鳞片和内芽为供试材料,研究种球的生长与乙烯释放量和NtERFs基因表达之间的关系。结果表明,多花水仙种球在3—5月间种球重量持续增加,为种球的膨大期。鳞片的乙烯释放量在5月中旬显著升高,内芽的乙烯释放量显著高于鳞片,在4月下旬出现释放高峰;内芽释放的乙烯可能与种球的膨大有关,且能够诱导鳞片中乙烯的释放,鳞片乙烯的释放可能会引起地上部叶片的黄化。实时荧光定量PCR结果表明,NtERF1、NtERF4和NtERF118的表达趋势与鳞片乙烯的释放量一致,可能参与了鳞片乙烯信号的响应,NtERF1可能是关键的响应因子。NtERF2、NtERF3、NtERF4和NtERF26基因则可能参与内芽乙烯信号的响应,NtERF2基因可能是内芽响应乙烯信号的主要因子,同时也参与了鳞片对内芽释放的乙烯信号的响应。本研究表明水仙种球在生长期能够响应乙烯信号,而乙烯的释放受到NtERFs的反馈调节。本研究结果为进一步研究水仙种球营养生长期乙烯的作用及其信号传导提供了研究基础。
Keyword ：
乙烯 乙烯 乙烯响应因子 乙烯响应因子 多花水仙 多花水仙 种球膨大期 种球膨大期
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| GB/T 7714 | 姜琳 , 孙兴 , 罗可欣 et al. 多花水仙种球膨大期对乙烯的响应研究 [J]. | 核农学报 , 2021 , 35 (06) : 1300-1306 . |
| MLA | 姜琳 et al. "多花水仙种球膨大期对乙烯的响应研究" . | 核农学报 35 . 06 (2021) : 1300-1306 . |
| APA | 姜琳 , 孙兴 , 罗可欣 , 郭志雄 , 陈桂信 , 佘文琴 et al. 多花水仙种球膨大期对乙烯的响应研究 . | 核农学报 , 2021 , 35 (06) , 1300-1306 . |
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Keyword ：
Citrus maxima Citrus maxima early season early season fruit breeding fruit breeding
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| GB/T 7714 | Pan, Tengfei , Huang, Peibin , Ye, Jianwen et al. 'Liuyuezao': A New Very Early-ripening Pummelo Cultivar [J]. | HORTSCIENCE , 2021 , 56 (12) : 1608-1609 . |
| MLA | Pan, Tengfei et al. "'Liuyuezao': A New Very Early-ripening Pummelo Cultivar" . | HORTSCIENCE 56 . 12 (2021) : 1608-1609 . |
| APA | Pan, Tengfei , Huang, Peibin , Ye, Jianwen , Pan, Dongming , Fu, Zhijun , Pan, Heli et al. 'Liuyuezao': A New Very Early-ripening Pummelo Cultivar . | HORTSCIENCE , 2021 , 56 (12) , 1608-1609 . |
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The first complete chloroplast (cp) genome of Narcissus 'Pink Charm' was sequenced and characterized using Illumina paired-end data. The assembled cp genome was 159,988 in length with a GC content of 37.82%. A total of 137 genes were annotated, consisting of 91 protein-coding genes, eight ribosomal RNA genes, and 38 transfer RNA genes. The phylogenetic position based on the cp genome data revealed that Narcissus 'Pink Charm' is more closely related to Narcissus poeticus than other relative species.
Keyword ：
complete chloroplast genome complete chloroplast genome Narcissus 'Pink Charm' Narcissus 'Pink Charm' phylogenetic analysis phylogenetic analysis
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| GB/T 7714 | Bian, Ana , Lu, Luanmei , Pan, Dongming . The complete chloroplast genome of Narcissus 'Pink Charm' [J]. | MITOCHONDRIAL DNA PART B-RESOURCES , 2021 , 6 (10) : 3089-3090 . |
| MLA | Bian, Ana et al. "The complete chloroplast genome of Narcissus 'Pink Charm'" . | MITOCHONDRIAL DNA PART B-RESOURCES 6 . 10 (2021) : 3089-3090 . |
| APA | Bian, Ana , Lu, Luanmei , Pan, Dongming . The complete chloroplast genome of Narcissus 'Pink Charm' . | MITOCHONDRIAL DNA PART B-RESOURCES , 2021 , 6 (10) , 3089-3090 . |
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In this study, the complete chloroplast (cp) genome of Narcissus tazetta var. chinensis cv 'Yulinglong' was sequenced and assembled by next-generation sequencing. The complete cp genome is 159,376 in length and contained 137 genes, consisting of 91 protein-coding genes, eight ribosomal RNA genes, and 38 transfer RNA genes. Phylogenetic analyses based on chloroplast genomes highly supported that 'Yulinglong' was evolutionarily close to Narcissus tazetta subsp.chinensis, which may provide more desirable information for the phylogenetic relationship between Narcissus tazetta var. chinensis and its relative species.
Keyword ：
chinensis chinensis complete chloroplast genome complete chloroplast genome double-petaled double-petaled Narcissus tazetta var Narcissus tazetta var phylogenetic analysis phylogenetic analysis Yulinglong Yulinglong
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| GB/T 7714 | Pu, Xiao-Long , Bian, A-Na , Pan, Dong-Ming . The complete chloroplast genome of 'Yulinglong' (Narcissus tazetta var. chinensis) with double-petaled in southeast of China [J]. | MITOCHONDRIAL DNA PART B-RESOURCES , 2021 , 6 (6) : 1765-1767 . |
| MLA | Pu, Xiao-Long et al. "The complete chloroplast genome of 'Yulinglong' (Narcissus tazetta var. chinensis) with double-petaled in southeast of China" . | MITOCHONDRIAL DNA PART B-RESOURCES 6 . 6 (2021) : 1765-1767 . |
| APA | Pu, Xiao-Long , Bian, A-Na , Pan, Dong-Ming . The complete chloroplast genome of 'Yulinglong' (Narcissus tazetta var. chinensis) with double-petaled in southeast of China . | MITOCHONDRIAL DNA PART B-RESOURCES , 2021 , 6 (6) , 1765-1767 . |
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