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学者姓名：吴刚
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Abstract ：
Organophosphorus insecticides (OPs), acting as serine phosphorylating agents in acetylcholinesterase (AChE), are highly effective neurotoxic insecticides. In our previous research, we found that six herbivorous pests and four ladybirds howed significantly higher AChE LC50 values than seven parasitoids and a predator (Epistrophe balteate), and that there was a significant correlation with the corresponding bimolecular rate constant (Ki) value. The Ki value of pests was much smaller than that of natural enemies and had a higher LC50 value.Then, we speculated that the low sensitivity of the pest AChE to OPs may be associated with its higher recovery and lower aging ability. In this work, the I50 and I90 were calculated, to determine the sensibility of AChE in ten representative species, including Plutella xylostella, Prodenia litura, Musca domestica, and Cavia porcellus, to paraoxon and malaoxon. The enzyme activities were measured at various time points, and kinetic calculations were used to obtain their spontaneous reactivation (Ks) and aging (Ka) constants, which were comprehensively compared. We conclude that the Ka and Ks of the AChE inhibited by OPs showed primarily species-specific correlations, and little correlation with the sensitivity to OPs. The differences in the AChE sensitivity to paraoxon among the ten species were much greater than in the sensitivity to malaoxon. Compared to paraoxon, malaoxon was more selective for Cavia porcellus. Coleoptera insects showed a stronger dephosphorylation ability than other insect groups. The recovery ability of phospho-AChE was stronger in mammals than in insects, which could be related to the low sensitivity of the AChE site of action to OPs. The Ka of the AChE inhibited by malaoxon was larger than that inhibited by paraoxon with the corresponding biomaterials, indicating that the OP type had a substantial relationship with the Ka of the AChE. We further discovered that, when insects were inhibited by OP, the tendency of AChE to undergo aging was greater than that of dephosphorylation. Overall, the study provides valuable information on the action mechanism of various OPs on AChE in several species, which could be used to further research into AChE and the potential dangers that organophosphates pose to animals.
Keyword ：
acetylcholinesterase acetylcholinesterase aging aging organophosphate organophosphate spontaneous reactivation spontaneous reactivation
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| GB/T 7714 | Gao, Mingwei , Ni, Zhongwen , Li, Guo et al. Study on Spontaneous Reactivation and Aging of Acetylcholinesterase Inhibited by Paraoxon and Malaoxon in Ten Species [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (18) . |
| MLA | Gao, Mingwei et al. "Study on Spontaneous Reactivation and Aging of Acetylcholinesterase Inhibited by Paraoxon and Malaoxon in Ten Species" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 18 (2023) . |
| APA | Gao, Mingwei , Ni, Zhongwen , Li, Guo , Wu, Gang , Huang, Binbin . Study on Spontaneous Reactivation and Aging of Acetylcholinesterase Inhibited by Paraoxon and Malaoxon in Ten Species . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (18) . |
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p53, DNA damage regulated gene (PDRG) and apoptosis-stimulating p53 protein 2 (ASPP2) are p53-related genes which can promote apoptosis. The full-length cDNA sequence of the Px-pdrg and Px-aspp2 genes were characterized and their mRNA expression dynamics under heat stress were studied in diamondback moth (DBM) Plutella xylostella collected from Fuzhou, China. The full-length cDNA of Px-pdrg and Px-aspp2 spans 721 and 4201 bp, containing 395 and 3216 bp of the open reading frame, which encode a putative protein comprising 130 and 1072 amino acids with a calculated molecular weight of 14.58 and 118.91 kDa, respectively. As compared to 25 degrees C, both Px-pdrg and Px-aspp2 were upregulated in chlorpyrifos-resistant (Rc) and -susceptible (Sm) strains of DBM adults and pupae under heat stress. In addition, Rc DBM showed a significantly higher expression level of Px-pdrg and Px-aspp2 in contrast to Sm DBM. The results indicate that high temperature can significantly promote apoptosis process, especially in Rc-DBM. Significant fitness cost in Rc-DBM might be associated with drastically higher transcript abundance of Px-pdrg and Px-aspp2 under the heat stress.
Keyword ：
Fitness cost Fitness cost heat stress heat stress Plutella xylostella Plutella xylostella Px-aspp2 Px-aspp2 Px-pdrg Px-pdrg
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| GB/T 7714 | Wang, Yu , Wang, Jing Nan , Chen, Xue Zhun et al. Heat stress-induced expression of Px-pdrg and Px-aspp2 in insecticide-resistant and -susceptible Plutella xylostella [J]. | BULLETIN OF ENTOMOLOGICAL RESEARCH , 2020 , 110 (2) : 177-184 . |
| MLA | Wang, Yu et al. "Heat stress-induced expression of Px-pdrg and Px-aspp2 in insecticide-resistant and -susceptible Plutella xylostella" . | BULLETIN OF ENTOMOLOGICAL RESEARCH 110 . 2 (2020) : 177-184 . |
| APA | Wang, Yu , Wang, Jing Nan , Chen, Xue Zhun , Hu, Qi Xing , Liu, Qi Qing , Wu, Gang . Heat stress-induced expression of Px-pdrg and Px-aspp2 in insecticide-resistant and -susceptible Plutella xylostella . | BULLETIN OF ENTOMOLOGICAL RESEARCH , 2020 , 110 (2) , 177-184 . |
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In our previous research, the fitness cost of resistance of the diamondback moth (DBM), Plutella xylostella found in insecticide-resistant DBM (Rc-DBM) under heat stress was based on heavier damage to wing veins when compared to insecticide-susceptible DBM (Sm-DBM). To investigate the molecular mechanism of the damage to the veins between Rc- and Sm-DBM, the full-length sequences of two related genes involved in the development of wing veins, fringe (Px-fng) and engrailed (Px-en) of DBM were cloned, and the mRNA expressions of both Px-fng and Px-en were studied. The Px-fng and Px-en cDNA contained 1038 bp and 1152 bp of open reading frames (ORFs), respectively, which encoded a putative protein comprising 345 and 383 amino acids with a calculated molecular weight of 39.59 kDa and 42.69 kDa. Significantly down regulated expressions of Px-fng and Px-en under heat stress were found in pupae and adults of Rc-DBM compared to Sm-DBM, and a result of higher damage to wing veins in Rc-DBM under heat stress. Based on RNAi experiments, significant inhibitions on expressions of Px-fng and Px-en in both Sm-DBM and Rc-DBM were found when the pupae were infected by dsFng or dsEn. Corresponding to these, infections of dsFng or dsEn resulted in significant decrease of eclosion rate and increase malformation rate of DBM. Our results suggest that the higher damage of wing veins in DBM might be related to the heavier inhibitions of Px-fng and Px-en expression, and the Px-fng and Px-en are involved in the development of wings and veins.
Keyword ：
engrailed engrailed fringe fringe heat stress heat stress resistant and susceptible Plutella xylostella resistant and susceptible Plutella xylostella
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| GB/T 7714 | Wang, Yu , Wang, Jingnan , Xia, Xiaofeng et al. Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae) [J]. | INSECTS , 2020 , 11 (5) . |
| MLA | Wang, Yu et al. "Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae)" . | INSECTS 11 . 5 (2020) . |
| APA | Wang, Yu , Wang, Jingnan , Xia, Xiaofeng , Wu, Gang . Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae) . | INSECTS , 2020 , 11 (5) . |
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The solid dispersion technique, which is widely used in the medical field, was applied to prepare a pesticide dosage form of emamectin benzoate (EM). The preparation, physicochemical characterization, aqueous solubility, release dynamics, photolytic degradation, bioactivity, and sustained-release effects of the prepared EM solid dispersions were studied by a solvent method, using polymer materials as the carriers. Water-soluble polyvinyl pyrrolidone (PVP) K30 and water-insoluble polyacrylic resin (PR)III were used as the carriers. The influence of various parameters, such as different EM:PVP-K30 and EM:PRIII feed ratios, solvent and container choices, rotational speed and mixing time effects on pesticide loading, and the entrapment rate of the solid dispersions were investigated. The optimal conditions for the preparation of EM-PVP-K30 solid dispersions required the use of methanol and a feed ratio between 1:1 and 1:50, along with a rotational speed and mixing time of 600 rpm and 60 min, respectively. For the preparation of EM-PRIII solid dispersions, the use of methanol and a feed ratio between 1:4 and 1:50 were required, in addition to the use of a porcelain mortar for carrying out the process. Under optimized conditions, the prepared EM-PVP-K30 solid dispersions resembled potato-like, round, and irregular structures with a jagged surface. In contrast, the EM-PRIII solid dispersions were irregular solids with a microporous surface structure. The results of X-ray powder diffraction (XRD), differential scanning calorimetry (DSC), ultraviolet (UV) spectrometry, and infrared (IR) spectrometry showed that the solid dispersions were formed by intermolecular hydrogen bonding. The solid dispersion preparation in PVP-K30 significantly improved the solubility and dissolution rate of EM, particularly the aqueous solubility, which reached a maximum of 37.5-times the EM technical solubility, when the feed ratio of 1:10 was employed to prepare the dispersion. Importantly, the wettable powder of EM-PVP-K30 solid dispersion enhanced the insecticidal activity of EM against the Plutella xylostella larvae. Furthermore, the solid dispersion preparation in PRIII afforded a significant advantage by prolonging the EM technical release in water at a pH below 7.0, especially when the PRIII content in solid dispersions was high. While the amplified toxicity of the wettable powder of EM-PRIII solid dispersions against the P. xylostella larvae showed no significant differences from that of the EM technical, the long-term toxicity under the field condition was much better than that of the commercially available EM 1.5% emulsifiable concentrate. Notably, solid dispersions with both the PVP-K30 and PRIII carriers reduced the effect of UV photolysis.
Keyword ：
insecticidal activity insecticidal activity polyacrylic resin polyacrylic resin polyvinyl pyrrolidone polyvinyl pyrrolidone solubilization solubilization stability stability sustained-release sustained-release
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| GB/T 7714 | Huang, Bin Bin , Liu, Dong Xu , Liu, De Kun et al. Application of Solid Dispersion Technique to Improve Solubility and Sustain Release of Emamectin Benzoate [J]. | MOLECULES , 2019 , 24 (23) . |
| MLA | Huang, Bin Bin et al. "Application of Solid Dispersion Technique to Improve Solubility and Sustain Release of Emamectin Benzoate" . | MOLECULES 24 . 23 (2019) . |
| APA | Huang, Bin Bin , Liu, Dong Xu , Liu, De Kun , Wu, Gang . Application of Solid Dispersion Technique to Improve Solubility and Sustain Release of Emamectin Benzoate . | MOLECULES , 2019 , 24 (23) . |
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BACKGROUND Long-term monitoring data is helpful to understand the fluctuation of susceptibility and pattern of cross resistance in insecticide resistance management. After the occurrence of imidacloprid resistance, the brown planthopper (BPH) has gradually developed resistance to thiamethoxam and clothianidin since 2010, but not to dinotefuran and nitenpyram. Here, we analyzed susceptibilities data of five neonicotinoids during 2005-2017 in East Asia and Vietnam to conduct cross-resistance patterns among neonicotinoids. To determine the factors of development of cross resistance in laboratory bioassays, we used the imidacloprid resistant and control strains that were selected from filed populations in the Philippines and Vietnam. RESULTS The Linear Mixed Models (LMM) analyses of insecticide susceptibility data showed that the slope values of imidacloprid resistance effects were 0.68 and 1.09 for resistance to thiamethoxam and clothianidin, respectively. Laboratory bioassay results showed that the LD50 values for thiamethoxam and clothianidin in resistant strains (1.4-5.5 mu g g(-1)) were 3.2-16.4 times higher than those in the control strains (0.28-1.5 mu g g(-1)). However, the increase in the LD50 values for imidacloprid was not related to that for dinotefuran and nitenpyram based on the results of the LMM analysis and laboratory bioassay. CONCLUSION Our results demonstrate that the development of imidacloprid resistance result in strong-cross resistance to some neonicotinoids, thiamethoxam and clothianidin, but not to others, dinotefuran and nitenpyram. We anticipate that our findings will be a starting point for understanding mechanism of the different trend of cross resistance by analyzing long-term susceptibility data and laboratory bioassays in insect pests. (c) 2019 Society of Chemical Industry
Keyword ：
cross resistance cross resistance imidacloprid imidacloprid insecticide resistance management insecticide resistance management laboratory selection laboratory selection Nilaparvata lugens Nilaparvata lugens
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| GB/T 7714 | Fujii, Tomohisa , Sanada-Morimura, Sachiyo , Oe, Takaho et al. Long-term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid-resistant brown planthopper Nilaparvata lugens [J]. | PEST MANAGEMENT SCIENCE , 2019 , 76 (2) : 480-486 . |
| MLA | Fujii, Tomohisa et al. "Long-term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid-resistant brown planthopper Nilaparvata lugens" . | PEST MANAGEMENT SCIENCE 76 . 2 (2019) : 480-486 . |
| APA | Fujii, Tomohisa , Sanada-Morimura, Sachiyo , Oe, Takaho , Ide, Manami , Dinh Van Thanh , Ho Van Chien et al. Long-term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid-resistant brown planthopper Nilaparvata lugens . | PEST MANAGEMENT SCIENCE , 2019 , 76 (2) , 480-486 . |
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Propylaea japonica (Thunberg) was a dominant species among the predacious ladybirds in the fields and active from March to November during a year in Fuzhou, China. Stability of insecticide resistance and vitality in adult P. japonica were investigated. The field ladybird P. japonica in Fuzhou, China, showed 9- to 16-fold resistance ratios to chlorpyrifos, 13- to 2,083-fold to methamidophos, 32- to 230-fold to fenvalerate, and 4- to 49-fold to avermectins, respectively, based on the field monitoring during 2004, 2009, and 2012, as compared with insecticide-susceptible F-39 progenies. The resistance levels in the field P. japonica were high during May and November but low during summer. The population growth tendency index in field P. japonica was 0.8-fold as high as that in insecticide-susceptible P. japonica. The field P. japonica also showed high tolerance to the insecticide as compared with pest Lipaphis erysimi Kaltenbach and two parasitoids Diaeretiella rapae and Pachyneuron aphidis. Stable insecticide resistance levels and high vitality were found first in adult P. japonica with 1-, 30-, or 60-d-old adults, or among the adults developed form the eggs produced by newly emerged adults or by 60-d-old adults. Increased activity of glutathione S-transferases, carboxylesterases, and cytochrome P460 monooxygenases might be involved in the resistance of P. japonica. The results indicated that, in certain areas, inclusion of P. japonica for pest control in the integrated pest management would be highly recommended.
Keyword ：
insecticide resistance insecticide resistance Propylaea japonica Propylaea japonica stability stability vitality vitality
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| GB/T 7714 | Wu, Gang , Wang, Yu , Wang, Jing Nan et al. Vitality and Stability of Insecticide Resistance in Adult Propylaea japonica (Coleoptera: Coccinellidae) [J]. | JOURNAL OF INSECT SCIENCE , 2018 , 18 (6) . |
| MLA | Wu, Gang et al. "Vitality and Stability of Insecticide Resistance in Adult Propylaea japonica (Coleoptera: Coccinellidae)" . | JOURNAL OF INSECT SCIENCE 18 . 6 (2018) . |
| APA | Wu, Gang , Wang, Yu , Wang, Jing Nan , Chen, Xue Zhun , Hu, Qi Xing , Yang, Yan Fan et al. Vitality and Stability of Insecticide Resistance in Adult Propylaea japonica (Coleoptera: Coccinellidae) . | JOURNAL OF INSECT SCIENCE , 2018 , 18 (6) . |
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Chlorpyrifos-resistant (Rc) Plutella xylostella (DBM) shows higher wing-vein injury than chlorpyrifos-susceptible (Sm) DBM under heat stress in our previous study. To investigate the toxicological mechanisms of the differences in injury of wing vein between Rc- and Sm-DBM collected from Fuzhou, China, total ten cDNA sequences of wing-development- related genes were isolated and characterized in DBM, including seven open reading frame (ORF) (ash1, ah2, ash3, ase, dpp, srf and dll encoded 187 amino acids, 231 aa, 223aa, 397aa, 423aa, 229aa and 299aa, respectively), and three partly sequences (salm, ser and wnt-1 encoded 614aa, 369aa and 388aa, respectively). The mRNA expression of the genes was inhibited in Rc- and Sm-DBM under heat stress, as compared with that an average temperature (25 degrees C). And, in general, significantly higher down-regulated expressions of the mRNA expression of the wing development-related genes were found in Rc-DBM as compared to those in Sm-DBM under heat stress. The results indicated that Sm-DBM displayed higher adaptability at high temperature because of significantly lower inhibition the mRNA expressions of wing-development-related genes. We suggest that significantly higher injury of wing vein showed in Rc-DBM under heat stress might be associated with the strong down-regulation of wing-development-related genes.
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| GB/T 7714 | Chen, Xue Zhun , Hu, Qi Xing , Liu, Qi Qing et al. Cloning of Wing-Development-Related Genes and mRNA Expression Under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutella xylostella [J]. | SCIENTIFIC REPORTS , 2018 , 8 . |
| MLA | Chen, Xue Zhun et al. "Cloning of Wing-Development-Related Genes and mRNA Expression Under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutella xylostella" . | SCIENTIFIC REPORTS 8 (2018) . |
| APA | Chen, Xue Zhun , Hu, Qi Xing , Liu, Qi Qing , Wu, Gang . Cloning of Wing-Development-Related Genes and mRNA Expression Under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutella xylostella . | SCIENTIFIC REPORTS , 2018 , 8 . |
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Characterization and gene cloning of acetylecholinesterase (AChE) in the insecticide-resistant (R) and -susceptible (S) insects have been reported in the past. However, the studies focused mostly on herbivorous pests, rather than predacious species, such as ladybird beetles. Using R and S Propylaea japonica (thunberg), a full-length cDNA sequence (2928 bp) of the ace1-type AChE gene was determined for the first time. The ace1 encoding a protein of 645 amino acids contained typical conserved motifs, such as FGESAG domains, catalytic triad, acyl pocket, oxyanino hole, choline binding site, peripheral anionic site, omega loop and conserved aromatic residues. R P. japonica displayed 50-times greater resistance to chlorpyrifos or mathamidophos with a significantly lower AChE sensitivity to paraoxon, malaoxon, chlorpyrifos or methamidophos than its S counterpart. Five amino acids in the ace1 of R P. japonica differed from those found in S P. japonica. One of them, F358S, located in the acyl-binding pocket, might play a crucial role in the resistance of the insect to organophosphates (OPs). Whereas, K493E and I538V, which were close to some of the conserved aromatic amino acids (i.e., H509, Y511, and W499) in the gorge, and G571R and T576A near C593 that formed the disulfide bonds with C471, might also involve in the change of insecticide resistance in P. japonica. AChE insensitivity and amino acid replacements, particularly F358S, might be the determining factors in the alteration of OPs-resistance in P. japonica.
Keyword ：
ace1 gene mutation ace1 gene mutation AChE sensitivity AChE sensitivity organophosphate resistance organophosphate resistance Propylaea japonica Propylaea japonica
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| GB/T 7714 | Wang, M. M. , Xing, L. Y. , Ni, Z. W. et al. Identification and characterization of ace1-type acetylcholinesterase in insecticide-resistant and -susceptible Propylaea japonica (Thunberg) [J]. | BULLETIN OF ENTOMOLOGICAL RESEARCH , 2018 , 108 (2) : 253-262 . |
| MLA | Wang, M. M. et al. "Identification and characterization of ace1-type acetylcholinesterase in insecticide-resistant and -susceptible Propylaea japonica (Thunberg)" . | BULLETIN OF ENTOMOLOGICAL RESEARCH 108 . 2 (2018) : 253-262 . |
| APA | Wang, M. M. , Xing, L. Y. , Ni, Z. W. , Wu, G. . Identification and characterization of ace1-type acetylcholinesterase in insecticide-resistant and -susceptible Propylaea japonica (Thunberg) . | BULLETIN OF ENTOMOLOGICAL RESEARCH , 2018 , 108 (2) , 253-262 . |
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Polycyclic aromatic hydrocarbons (PAHs) are a group of persistent organic pollutants primarily formed from the incomplete combustion of carbonaceous materials, and have adverse effects on human health. In this study, we investigated whether pyrene, a PAH consisting of 4 fused benzene rings, has adverse effects on rat. Adult male Sprague-Dawly rats were treated daily by oral gavage with vehicle (corn oil) or pyrene at doses of 375, 750, 1500, or 2200 mg/kg/day for 4 days. The results showed that pyrene caused hepatotoxicity in rats. When compared with the control group, relative liver weights, plasma alanine aminotransferase, and direct bilirubin levels significantly increased after pyrene exposure. Hepatocyte swelling and degeneration and decreased hepatic total glutathione (GSH) levels were also found in pyrene-exposed rats. We further observed that mRNA levels of several hepatic metabolizing enzymes regulated by constitutive androstane receptor (CAR) such as CYP2B1 and CYP2B2 significantly increased in pyrene-exposed rats. These results suggest that decreased GSH levels, elevated hepatic metabolizing enzyme gene expression, and CAR activation are important contributors for pyrene-induced hepatotoxicity in rats. Additionally, we found pyrene significantly induced plasma inflammatory indices including white blood cell and lymphocyte counts. We also observed that pyrene exposure increased relative weight of kidneys and disrupted kidney function with elevated urea and creatinine levels in rats.
Keyword ：
constitutive androstane receptor constitutive androstane receptor cytochromes P450 cytochromes P450 glutathione glutathione hepatotoxicity hepatotoxicity pyrene pyrene
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| GB/T 7714 | Zhu, Jiayin , Zhao, Li-Yang , Wang, Xiao-Xiao et al. Identification of hepatotoxicity and renal dysfunction of pyrene in adult male rats [J]. | ENVIRONMENTAL TOXICOLOGY , 2018 , 33 (12) : 1304-1311 . |
| MLA | Zhu, Jiayin et al. "Identification of hepatotoxicity and renal dysfunction of pyrene in adult male rats" . | ENVIRONMENTAL TOXICOLOGY 33 . 12 (2018) : 1304-1311 . |
| APA | Zhu, Jiayin , Zhao, Li-Yang , Wang, Xiao-Xiao , Shi, Zhe , Zhang, Yang , Wu, Gang et al. Identification of hepatotoxicity and renal dysfunction of pyrene in adult male rats . | ENVIRONMENTAL TOXICOLOGY , 2018 , 33 (12) , 1304-1311 . |
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Glyphosate, one of the most popular herbicides world-wide, is used as an active ingredient in many commercial formulations. So far, many studies have focused on reproductive toxicity on glyphosate which may trigger epigenetic changes through endocrine-mediated mechanisms. Generally, it is critical to identify the glyphosate poisoning status in order to minimize health risks. This paper proposed a machine learning approach using 110 rats samples to identify the glyphosate poisoning status. All rats were randomly divided into 2 groups (n = 55 each) in the study. The rats in the blank control group were given 0.9% sodium chloride solution orally for 15 days, while the other groups were administered orally at the dosage of 0.5g/kg of glyphosate once per day for 15 days consecutively. Consequently, the potential of an enhanced fuzzy k nearest neighbor approach was explored through blood routine test to recognize the glyphosate poisoning condition based on the blood indices. A real-life data set was used to evaluate the effectiveness of the proposed method in terms of classification accuracy (ACC), sensitivity, specificity, and Matthews Correlation Coefficients (MCC). The results demonstrated that there were significant differences in blood routine test indices between the control and the glyphosate groups (p-value < 0.01). It was observed in the feature selection that the most important correlated indices were white blood cell, lymphocyte, intermediate cell, and neutrophil granulocyte. A comparative study with support vector machine and artificial neural networks had shown that the proposed approach could achieve much more promising results with 95.45% ACC, 0.90 MCC, 98.89% sensitivity, and 88.89% specificity. The empirical analysis verifies that the proposed method is promising to act as a new, accurate method for identification of the glyphosate poisoning status in subjects.
Keyword ：
feature selection feature selection fuzzy k nearest neighbor fuzzy k nearest neighbor glyphosate poisoning status identification glyphosate poisoning status identification Machine learning Machine learning
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| GB/T 7714 | Zhu, Jiayin , Zhao, Xuehua , Li, Huaizhong et al. An Effective Machine Learning Approach for Identifying the Glyphosate Poisoning Status in Rats Using Blood Routine Test [J]. | IEEE ACCESS , 2018 , 6 : 15653-15662 . |
| MLA | Zhu, Jiayin et al. "An Effective Machine Learning Approach for Identifying the Glyphosate Poisoning Status in Rats Using Blood Routine Test" . | IEEE ACCESS 6 (2018) : 15653-15662 . |
| APA | Zhu, Jiayin , Zhao, Xuehua , Li, Huaizhong , Chen, Huiling , Wu, Gang . An Effective Machine Learning Approach for Identifying the Glyphosate Poisoning Status in Rats Using Blood Routine Test . | IEEE ACCESS , 2018 , 6 , 15653-15662 . |
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