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学者姓名：何碧珠
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Abstract ：
Bougainvillea is valued for its ornamental and medicinal properties, but its current propagation primarily relies on cuttings, which suffer from low efficiency. Although tissue culture offers faster propagation and is not limited by seasonal properties, the technical system remains underdeveloped. This study applies the Response Surface Methodology (RSM) to optimize the axillary bud induction and rooting stages of Bougainvillea tissue culture. By fitting a multivariate quadratic regression model to determine the relationship between factors and response values, the study aimed to refine the tissue culture system for Bougainvillea. The main findings of this study are as follows: (1) In the axillary bud induction stage, the optimal induction medium was MS (Murashige and Skoog) + 6-BA (6-Benzylaminopurine) 2.534 mg L- 1 + NAA (1-Naphthaleneacetic acid) 0.1 mg L-1, achieving a bud induction rate of 93.3 %. (2) The ideal rooting medium consists of 0.66 MS + IBA (Indole-3-butyric acid) 2.0 mg L- 1 + NAA 0.09 mg L-1. Upon optimizing the culture system, the bud induction rate during the primary culture stage rose to 93.3 %, and the rooting rate during the rooting stage reached 66.7 %. In conclusion, the optimization of culture media using a multifactorial approach proved more effective than the traditional twofactor method, and more accurately optimizes the media of different stages, which provides valuable insights for improving the Bougainvillea tissue culture system.
Keyword ：
Bougainvillea Bougainvillea Response surface analysis Response surface analysis Tissue culture Tissue culture
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| GB/T 7714 | Li, Yu , Lin, Wei , Huang, Jing et al. Optimization of Bougainvillea tissue culture system by response surface methodology [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 226 . |
| MLA | Li, Yu et al. "Optimization of Bougainvillea tissue culture system by response surface methodology" . | INDUSTRIAL CROPS AND PRODUCTS 226 (2025) . |
| APA | Li, Yu , Lin, Wei , Huang, Jing , Ding, Jintao , Li, Jing , He, Bizhu et al. Optimization of Bougainvillea tissue culture system by response surface methodology . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 226 . |
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本发明涉及三角梅嫁接装置技术领域，公开了一种辅助三角梅高位嫁接造型的装置及其方法，包括：基座；导向机构，基座的内部设有导向机构；花盆，基座的内部通过导向机构设有花盆；嫁接机构，花盆的内部滑动连接有嫁接杆，嫁接杆的外侧壁拆卸连接有引导杆，引导杆的外侧壁设有嫁接机构，所述嫁接机构包含有嫁接环、第一齿轮环、第一锥齿轮、螺纹杆和嫁接块，所述引导杆的外侧壁设有嫁接环；通过导向机构和锁定机构可以根据不同角度的枝干进行角度调节，从而控制后期的生长方向，为后期的造型进行初步的把控，且通过嫁接机构可以快速的调节需要的高度然后对枝干和三角梅进行固定，便于后期的嫁接牢固，提高嫁接成功率。
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| GB/T 7714 | 郭梨锦 , 薛昕宇 , 林蔚 et al. 一种辅助三角梅高位嫁接造型的装置及其方法 : CN202510999349.9[P]. | 2025-07-21 . |
| MLA | 郭梨锦 et al. "一种辅助三角梅高位嫁接造型的装置及其方法" : CN202510999349.9. | 2025-07-21 . |
| APA | 郭梨锦 , 薛昕宇 , 林蔚 , 何碧珠 , 王宗博 , 杨宏宇 . 一种辅助三角梅高位嫁接造型的装置及其方法 : CN202510999349.9. | 2025-07-21 . |
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Abstract ：
Cutting propagation is a crucial technique for woody ornamental plant reproduction. While the plant growth regulator naphthaleneacetic acid (NAA) and the beneficial fungus Piriformospora indica (Pi) are vital for adventitious root formation and cutting survival, the underlying mechanisms of their synergistic effects remain unclear. This study investigated the impact of NAA and Pi on rooting in cuttings of three distinct woody ornamentals: Rhododendron simsii, Jasminum sambac, and Rosa chinensis. Three treatments were applied: control group (CK, direct cutting), Pi inoculation (Pi), and NAA soaking followed by Pi inoculation (NAA+Pi). The results demonstrated that compared to CK, NAA+Pi significantly increased rooting rate (1.9 %-85.0 %) and survival rate (10.5 %-36.6 %), improved chlorophyll and free amino acid content, and enhanced plant height and fresh weight. Notably, NAA+Pi dramatically upregulated the expression of the key root development gene WOX4 by 2.6-4.0 fold, indicating a synergistic activation of WOX4 gene expression. The increased free amino acid content in the NAA+Pi treatment suggests enhanced secondary metabolism, providing essential resources for WOX expression and root development. All species demonstrated notable improvement, despite the fact that R. chinensis saw the most noticeable impacts. This underscores the considerable potential of using both NAA and Pi together to maximize the proliferation of woody ornamental cuttings. This research offers new insights for improving cutting propagation techniques.
Keyword ：
Naphthalene acetic acid Naphthalene acetic acid Piriformospora indica Piriformospora indica Rooting survival Rooting survival Woody ornamentals Woody ornamentals WOX gene WOX gene
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| GB/T 7714 | Tang, Xuexiao , Wang, Zongbo , Zong, Fanqi et al. Impact of naphthalene acetic acid and Piriformospora indica on WOX gene expression and rooting in woody ornamentals [J]. | SCIENTIA HORTICULTURAE , 2025 , 350 . |
| MLA | Tang, Xuexiao et al. "Impact of naphthalene acetic acid and Piriformospora indica on WOX gene expression and rooting in woody ornamentals" . | SCIENTIA HORTICULTURAE 350 (2025) . |
| APA | Tang, Xuexiao , Wang, Zongbo , Zong, Fanqi , Xue, Xinyu , Gao, Jiayuan , Li, Shanghuan et al. Impact of naphthalene acetic acid and Piriformospora indica on WOX gene expression and rooting in woody ornamentals . | SCIENTIA HORTICULTURAE , 2025 , 350 . |
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本发明公开了一种促进茶园土壤固碳减排的镁肥施用装置，涉及施肥领域，包括小推车、设置于小推车顶部的储料桶、固定连接在储料桶底部的排料机构，以及固定连接在储料桶顶部的混合箱，还包括与储料桶相连接的打散机构、与混合箱相连接的搅拌机构，以及设置于储料桶与混合箱内部的分隔机构；打散机构包括贯穿储料桶侧壁并与侧壁转动连接的驱动轴，以及固定连接在驱动轴外侧壁用于带动所述驱动轴旋转的传动杆；一种促进茶园土壤固碳减排的镁肥施用装置的施用方法，包括配料、混合、下料与再混合以及施肥。本发明为一种促进茶园土壤固碳减排的镁肥施用装置及施用方法，通过将振动力转为驱动轴的驱动力，来提高行驶的稳定性。
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| GB/T 7714 | 郭梨锦 , 林蔚 , 廖鹏辉 et al. 一种促进茶园土壤固碳减排的镁肥施用装置及施用方法 : CN202411844517.9[P]. | 2024-12-16 . |
| MLA | 郭梨锦 et al. "一种促进茶园土壤固碳减排的镁肥施用装置及施用方法" : CN202411844517.9. | 2024-12-16 . |
| APA | 郭梨锦 , 林蔚 , 廖鹏辉 , 吴良泉 , 王培杰 , 何正嘉 et al. 一种促进茶园土壤固碳减排的镁肥施用装置及施用方法 : CN202411844517.9. | 2024-12-16 . |
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本发明涉及种植花盆技术领域，公开了一种三角梅提根造型装置及使用方法，其结构包括花盆、无底盆、支架，本发明通过在三角梅的种植花盆上增设有无底盆，借助无底盆通过若干可拼接的扩展环组装而成，能够依据所需对三角梅根系的提根高度，进行扩展环数量的叠加，从而能够对不同高度的根系进行提根，增加了提根造型时的多变性和可适应性，且在花盆上增设有约束框，将约束框放置在花盆中心需要限制根系向下生长的区域，借助其上的孔洞能允许三角梅根系的毛细根穿过吸收花盆内部土壤的养分水分，但通过约束框能够物理阻隔主根向外深扎，迫使根系向预设的提拉区域发展，能从根源上减少无效根系生长，集中能量于造型部位，提高提根造型的效率。
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| GB/T 7714 | 郭梨锦 , 余菁慧 , 曾歆然 et al. 一种三角梅提根造型装置及使用方法 : CN202510990104.X[P]. | 2025-07-18 . |
| MLA | 郭梨锦 et al. "一种三角梅提根造型装置及使用方法" : CN202510990104.X. | 2025-07-18 . |
| APA | 郭梨锦 , 余菁慧 , 曾歆然 , 林蔚 , 何碧珠 . 一种三角梅提根造型装置及使用方法 : CN202510990104.X. | 2025-07-18 . |
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由于掠夺式采挖、生境破碎化等人为因素对生态环境的严重破坏，使多数野生流苏贝母兰(Coelogyne fimbriata Lindl.)植物处于濒危状态，现有贝母兰的繁殖方式主要为分株繁殖，一般每隔2～3年分株1次，繁殖周期过长，速度缓慢，因此依靠自然恢复很难保护野生资源。而现有研究对于贝母兰以及流苏贝母兰的无菌播种繁殖探究较少，实际操作中仍存在不少难题，因此急需探究流苏贝母兰种子无菌萌发的最佳条件，以提高种苗繁育的成功率。本研究以流苏贝母兰蒴果为外植体，对流苏贝母兰种子萌发的消毒时间、消毒剂种类、基本培养基以及光照条件等因素进行测定和分析。结果表明，流苏贝母兰种子的最适消毒方法为用0.1%氯化汞消毒处理10 min或2%NaClO处理14 min后用无菌水冲洗；诱导种子萌发的最适培养基为1/2MS+1.5 mg/L 6-BA+0.5 mg/L NAA，最适pH值为5.6～5.7，最适温度为(23±2)℃。本研究有利于流苏贝母兰的种苗繁育和种质资源保护，并可为兰科植物种子的无菌萌发及离体快繁技术的应用提供理论及技术支持。
Keyword ：
培养基 培养基 外源植物激素 外源植物激素 无菌播种 无菌播种 流苏贝母兰 流苏贝母兰 消毒 消毒
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| GB/T 7714 | 李茂金 , 何荣晓 , 王鸿荏 et al. 诱导流苏贝母兰种子无菌萌发的最佳条件 [J]. | 分子植物育种 , 2025 , 23 (22) : 7571-7579 . |
| MLA | 李茂金 et al. "诱导流苏贝母兰种子无菌萌发的最佳条件" . | 分子植物育种 23 . 22 (2025) : 7571-7579 . |
| APA | 李茂金 , 何荣晓 , 王鸿荏 , 何碧珠 , 郭梨锦 . 诱导流苏贝母兰种子无菌萌发的最佳条件 . | 分子植物育种 , 2025 , 23 (22) , 7571-7579 . |
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Tissue culture processes have been established for Dendrobium wilsonii Rolfe, a valued ornamental and medicinal orchid. Pods aged 180 days served as seed sources. These seeds underwent surface sterilization and were then aseptically germinated on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 Benzyl adenine (BA), 0.5 mg L-1 Naphthalene acetic acid (NAA), 20 g L-1 Sucrose (Su), 7 g L-1 Agar, 1 g L-1 Activated charcoal (AC), 1 g L-1 Peptone and 1 g L-1 Hyponex No.1 incubated in a growth chamber at 25.2 degrees C with a 12-hour photoperiod and a light intensity of 2200 lx. Multiple shoot induction and proliferation was on MS medium in vitro produced shoots were rooted on MS medium with Hyponex No.1, 25 g L-1 Su, 1 g L-1 AC and 1.5 g L-1 Peptone. Peptone enhanced seedling growth and promoted clumped shoot development. The establishment of Dendrobium wilsonii Rolfe regeneration system provides a methodology for conserving germplasm resources and for commercial propagules production.
Keyword ：
Dendrobium wilsonii Rolfe Dendrobium wilsonii Rolfe Regeneration system Regeneration system Tissue culture Tissue culture
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| GB/T 7714 | Lin, Wei , Li, Yu , Liang, Jincheng et al. Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system [J]. | SCIENTIA HORTICULTURAE , 2024 , 324 . |
| MLA | Lin, Wei et al. "Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system" . | SCIENTIA HORTICULTURAE 324 (2024) . |
| APA | Lin, Wei , Li, Yu , Liang, Jincheng , Liu, Yufang , Chen, Peiqiang , He, Bizhu et al. Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system . | SCIENTIA HORTICULTURAE , 2024 , 324 . |
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为了建立草珊瑚再生体系，加快其繁殖速度，缩短繁育周期，为草珊瑚的组培工厂化育苗和产业化发展利用提供一定的参考，本研究以草珊瑚茎段为外植体，通过控制外植体取材时间，消毒试剂类型及消毒时长，培养基类型以及外源激素浓度配比的不同，从初代培养到继代培养再到生芽、壮苗与生根和试管苗移栽，以此获得最佳培养条件。结果表明：草珊瑚外植体采摘最佳时间为3～5月；腋芽茎段消毒以2%NaClO处理14 min或0.1%HgCl
Keyword ：
丛生芽 丛生芽 初代诱导 初代诱导 生根诱导 生根诱导 继代增殖 继代增殖 草珊瑚 草珊瑚
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| GB/T 7714 | 戴凌 , 何荣晓 , 王友雄 et al. 草珊瑚再生体系建立及繁殖分析 [J]. | 分子植物育种 , 2024 , 22 (21) : 7166-7173 . |
| MLA | 戴凌 et al. "草珊瑚再生体系建立及繁殖分析" . | 分子植物育种 22 . 21 (2024) : 7166-7173 . |
| APA | 戴凌 , 何荣晓 , 王友雄 , 何碧珠 , 郭梨锦 . 草珊瑚再生体系建立及繁殖分析 . | 分子植物育种 , 2024 , 22 (21) , 7166-7173 . |
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Qinting Lake Park has effectively imported Rhododendron varieties from Zhejiang Province. The analytic hierarchy process was employed to devise an evaluation framework to evaluate the ornamental and adaptive features of these species. Subsequently, we conducted a standardized evaluation of 24 species for their ornamental and adaptive traits under controlled cultivation conditions. The findings indicated that the percentage of ornamental flowers in the first-level index was significantly greater than the other two factors, indicating that the ornamental value of flowers was the most important in the evaluation of Rhododendron ornamental value. Among the secondary indicators, the proportion of flower color and flower weight was significantly higher than that of other factors, which had the greatest impact on the evaluation results. The 24 Rhododendron species were classified into two grades based on their ornamental value, as determined by index weights and scoring standards. Rhododendron 'Xueqing', Rhododendron 'Big Qinglian', and Rhododendron 'Jinyang No. 9' exhibited superior ornamental value and demonstrated more favorable suitability for garden applications.
Keyword ：
adaptability adaptability analytic hierarchy process analytic hierarchy process comprehensive evaluation comprehensive evaluation ornamentality ornamentality Rhododendron Rhododendron
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| GB/T 7714 | Liang, Jincheng , Chen, Yaoli , Tang, Xuexiao et al. Comprehensive Evaluation of Appreciation of Rhododendron Based on Analytic Hierarchy Process [J]. | PLANTS-BASEL , 2024 , 13 (4) . |
| MLA | Liang, Jincheng et al. "Comprehensive Evaluation of Appreciation of Rhododendron Based on Analytic Hierarchy Process" . | PLANTS-BASEL 13 . 4 (2024) . |
| APA | Liang, Jincheng , Chen, Yaoli , Tang, Xuexiao , Lu, Yin , Yu, Jinghui , Wang, Zongbo et al. Comprehensive Evaluation of Appreciation of Rhododendron Based on Analytic Hierarchy Process . | PLANTS-BASEL , 2024 , 13 (4) . |
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本发明公开了一种利用印度梨形孢与镁协同作用提高杜鹃耐热性的方法，其包括：将印度梨形孢固体培养物施于杜鹃根系周围的土壤中，然后以10天浇灌50ml的参数在杜鹃根系土壤施用两次印度梨形孢液体菌液，再在杜鹃根系周围的土壤施用MgSO4·7H2O两次，每次间隔5天；再令杜鹃在初始高温胁迫温度下培育，再按预设条件逐步提高胁迫温度，直至达到目标高温胁迫温度，高温胁迫过程中按预设条件施用MgSO4·7H2O和印度梨形孢液体菌液，最后按预设条件筛选获得耐热性提高的杜鹃，本方案可提高杜鹃耐热性，改善杜鹃在热胁迫环境下的生长情况，促进杜鹃在园林绿化中更广泛的应用，为杜鹃的栽培和管理提供科学依据，提高其在高温环境下的生长表现，促进园林植物的可持续发展。
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| GB/T 7714 | 郭梨锦 , 郭云欣 , 林蔚 et al. 一种利用印度梨形孢与镁协同作用提高杜鹃耐热性的方法 : CN202411179812.7[P]. | 2024-08-27 . |
| MLA | 郭梨锦 et al. "一种利用印度梨形孢与镁协同作用提高杜鹃耐热性的方法" : CN202411179812.7. | 2024-08-27 . |
| APA | 郭梨锦 , 郭云欣 , 林蔚 , 施杰 , 何碧珠 . 一种利用印度梨形孢与镁协同作用提高杜鹃耐热性的方法 : CN202411179812.7. | 2024-08-27 . |
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